BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122169.4 - phase: 0
(165 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLG_HRV16 (Q82122) Genome polyprotein [Contains: Coat protein V... 34 0.17
POLG_HRV89 (P07210) Genome polyprotein [Contains: Coat protein V... 33 0.38
UBC6_HUMAN (P51965) Ubiquitin-conjugating enzyme E2 E1 (EC 6.3.2... 32 0.50
GCN2_YEAST (P15442) Serine/threonine-protein kinase GCN2 (EC 2.7... 32 0.65
UBC6_MOUSE (P52482) Ubiquitin-conjugating enzyme E2 E1 (EC 6.3.2... 32 0.85
SNE1_HUMAN (Q8NF91) Nesprin 1 (Nuclear envelope spectrin repeat ... 32 0.85
YSR2_CAEEL (Q09950) Hypothetical protein F59B10.2 in chromosome II 31 1.1
UVRB_MYCPN (P75558) UvrABC system protein B (UvrB protein) (Exci... 30 1.9
YCAK_CAEEL (Q18563) Hypothetical protein C41G11.3 in chromosome X 30 2.5
RGP1_HUMAN (P46060) Ran GTPase-activating protein 1 30 2.5
NO60_DROME (O44081) Nucleolar protein AT band 60B (Minifly protein) 30 2.5
Z410_MOUSE (Q8BKX7) Zinc finger protein 410 (Zinc finger protein... 30 3.2
POLG_HRV1B (P12916) Genome polyprotein [Contains: Coat protein V... 30 3.2
UVRB_MYCGE (P47319) UvrABC system protein B (UvrB protein) (Exci... 29 4.2
RGP1_MOUSE (P46061) Ran GTPase-activating protein 1 29 4.2
PAC2_XENLA (Q9DDA9) Protein kinase C and casein kinase substrate... 29 4.2
ARRI_ARATH (Q9FGT7) Two-component response regulator ARR18 29 4.2
SYM_CLOAB (Q97EW5) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 29 5.5
RGYR_SULSH (P74759) Reverse gyrase [Includes: Helicase (EC 3.6.1... 29 5.5
DMP1_RAT (P98193) Dentin matrix acidic phosphoprotein 1 precurso... 29 5.5
>POLG_HRV16 (Q82122) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core
protein P2A); Core protein P2B; Core protein P2C; Core
protein P3A; Genome
Length = 2153
Score = 33.9 bits (76), Expect = 0.17
Identities = 27/111 (24%), Positives = 48/111 (42%), Gaps = 14/111 (12%)
Query: 23 TKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQELTGQYAESPPNPSRFQ 82
T + K KK+ ++P+ V+ P K+ + F A + PP+P F
Sbjct: 1196 TNIIKQKKR-------SEPVAVMIHGPPGTGKSITTSFLARMITNESDIYSLPPDPKYFD 1248
Query: 83 EFD------VNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDDKGKPSEA 127
+D ++D + GG + + C +V +P + D DKGKP ++
Sbjct: 1249 GYDNQSVVIMDDIMQNPGGEDMTLFCQMVS-SVTFIPPMADLPDKGKPFDS 1298
>POLG_HRV89 (P07210) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core
protein P2A); Core protein P2B; Core protein P2C; Core
protein P3A; Genome
Length = 2164
Score = 32.7 bits (73), Expect = 0.38
Identities = 32/131 (24%), Positives = 52/131 (39%), Gaps = 10/131 (7%)
Query: 39 NKPIKVVYISNPMKVKTSASEFRALVQELTGQYAESPPNPSRFQEFD-----VNDSGTDQ 93
N+P+ V+ +P K+ A+ A + + PP+P F +D + D
Sbjct: 1218 NEPVAVLIHGSPGTGKSLATSVLARMLTVETDIYSLPPDPKYFDGYDQQSVVIMDDIMQN 1277
Query: 94 GGCENMMDCDKSDQTVVGVPSLVDPDDKGKPSEAGSSNESFDEDVLLMPEMMDNIWDLLP 153
E+M + +V +P + D DKGKP + S + +L P + LP
Sbjct: 1278 PSGEDMTLFCQMVSSVPFIPPMADLPDKGKPFTSKFVLASTNHTLLTPPTVSS-----LP 1332
Query: 154 TSAFYESFQLD 164
A F LD
Sbjct: 1333 AMARRFYFDLD 1343
>UBC6_HUMAN (P51965) Ubiquitin-conjugating enzyme E2 E1 (EC
6.3.2.19) (Ubiquitin-protein ligase E1) (Ubiquitin
carrier protein E1) (UbcH6)
Length = 193
Score = 32.3 bits (72), Expect = 0.50
Identities = 25/78 (32%), Positives = 37/78 (47%), Gaps = 12/78 (15%)
Query: 2 DSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFR 61
D S +STSSS S+ Q+T + PKKK++ KV N + TSA +
Sbjct: 3 DDDSRASTSSSSSSSSNQQTEKETNTPKKKES---------KVSMSKNSKLLSTSAKRIQ 53
Query: 62 ALVQELTGQYAESPPNPS 79
+ ++T + PPN S
Sbjct: 54 KELADIT---LDPPPNCS 68
>GCN2_YEAST (P15442) Serine/threonine-protein kinase GCN2 (EC
2.7.1.37)
Length = 1590
Score = 32.0 bits (71), Expect = 0.65
Identities = 31/138 (22%), Positives = 59/138 (42%), Gaps = 15/138 (10%)
Query: 2 DSISTSSTSSSVISTVQQKTPTKLTKPKKKK---NNNNTYNKPIKVVYISNPMKVKTSAS 58
D + S+ V++ QQ + K++ N+ KP+K+ +S + +
Sbjct: 1354 DMLRDCSSVDDVVTGAQQDGIDWILLIKQQAYPLTNHKRKYKPLKIKKLSTNVDIDLDLD 1413
Query: 59 EFRALVQELTGQYAESPPNPS--------RFQEFDVNDS--GTDQGGCENMMDCDKSDQT 108
EF L Q+ TG +S N S F+ +D N S + +G ++++ ++Q
Sbjct: 1414 EFLTLYQQETGN--KSLINDSLTLGDKADEFKRWDENSSAGSSQEGDIDDVVAGSTNNQK 1471
Query: 109 VVGVPSLVDPDDKGKPSE 126
V+ VP++ K E
Sbjct: 1472 VIYVPNMATRSKKANKRE 1489
>UBC6_MOUSE (P52482) Ubiquitin-conjugating enzyme E2 E1 (EC
6.3.2.19) (Ubiquitin-protein ligase E1) (Ubiquitin
carrier protein E1) (UbcM3)
Length = 193
Score = 31.6 bits (70), Expect = 0.85
Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 12/78 (15%)
Query: 2 DSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFR 61
D S +STSSS S+ Q+T + + PKKK++ KV N + TSA +
Sbjct: 3 DDDSRASTSSSSSSSSNQQTEKEGSTPKKKES---------KVSMSKNSKLLSTSAKRIQ 53
Query: 62 ALVQELTGQYAESPPNPS 79
+ ++T + PPN S
Sbjct: 54 KELADIT---LDPPPNCS 68
>SNE1_HUMAN (Q8NF91) Nesprin 1 (Nuclear envelope spectrin repeat
protein 1) (Synaptic nuclear envelope protein 1)
(Syne-1) (Myocyte nuclear envelope protein 1) (Myne-1)
(Enaptin)
Length = 8797
Score = 31.6 bits (70), Expect = 0.85
Identities = 38/131 (29%), Positives = 60/131 (45%), Gaps = 18/131 (13%)
Query: 5 STSSTSSSVISTVQQKTPTKLTKPK-KKKNNNNTYNKPIKVVYIS----NPMKVKTSASE 59
S SS++SSV S V +TP+ +K K K N +K V + ++VK
Sbjct: 147 SLSSSASSVDSIVSSETPSPPSKRKVTTKIQGNAKKALLKWVQYTAGKQTGIEVKDFGKS 206
Query: 60 FRALVQELTGQYAESPPNPSRFQEFDVNDSGTDQGGC--ENMMDCDKSDQTVVGVPSLVD 117
+R+ V + +A P ++ D T +G EN+ D +T +G+P L+D
Sbjct: 207 WRSGVAFHSVIHAIRP---------ELVDLETVKGRSNRENLEDAFTIAETELGIPRLLD 257
Query: 118 PD--DKGKPSE 126
P+ D KP E
Sbjct: 258 PEDVDVDKPDE 268
>YSR2_CAEEL (Q09950) Hypothetical protein F59B10.2 in chromosome II
Length = 482
Score = 31.2 bits (69), Expect = 1.1
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 6 TSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKP 41
+ +SSS ST+ + TP KL KP+K K + KP
Sbjct: 273 SEKSSSSSASTISKVTPKKLDKPQKTKKPDKKRAKP 308
>UVRB_MYCPN (P75558) UvrABC system protein B (UvrB protein)
(Excinuclease ABC subunit B)
Length = 657
Score = 30.4 bits (67), Expect = 1.9
Identities = 20/85 (23%), Positives = 41/85 (47%), Gaps = 5/85 (5%)
Query: 7 SSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFR--ALV 64
S I Q++ ++ ++ T KPI ++ P+K+KT +SE + AL+
Sbjct: 562 SEQMDKAIKETQRRRTIQMAYNEQHHKTPMTVQKPIT---LNQPIKLKTKSSEQQKAALI 618
Query: 65 QELTGQYAESPPNPSRFQEFDVNDS 89
++LT + ++ N + ++ DS
Sbjct: 619 KQLTKEMKQAAANQNYELAIEIRDS 643
>YCAK_CAEEL (Q18563) Hypothetical protein C41G11.3 in chromosome X
Length = 737
Score = 30.0 bits (66), Expect = 2.5
Identities = 18/65 (27%), Positives = 29/65 (43%)
Query: 3 SISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRA 62
S + S S + +S + KT K+ KP+ +K + I S PM V+T + R
Sbjct: 550 SNNVSEPSKNRLSIFKSKTEKKVVKPESEKEKLKPRSDDIPTTSTSTPMAVETKPNGSRT 609
Query: 63 LVQEL 67
+ L
Sbjct: 610 TEEPL 614
>RGP1_HUMAN (P46060) Ran GTPase-activating protein 1
Length = 587
Score = 30.0 bits (66), Expect = 2.5
Identities = 17/86 (19%), Positives = 38/86 (43%)
Query: 52 KVKTSASEFRALVQELTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVG 111
K+K F + ++ AE+ + + ++ D+N + + GCE + + +
Sbjct: 294 KLKELNLSFCEIKRDAALAVAEAMADKAELEKLDLNGNTLGEEGCEQLQEVLEGFNMAKV 353
Query: 112 VPSLVDPDDKGKPSEAGSSNESFDED 137
+ SL D +D+ + E E +E+
Sbjct: 354 LASLSDDEDEEEEEEGEEEEEEAEEE 379
>NO60_DROME (O44081) Nucleolar protein AT band 60B (Minifly protein)
Length = 508
Score = 30.0 bits (66), Expect = 2.5
Identities = 13/33 (39%), Positives = 21/33 (63%)
Query: 4 ISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNN 36
+STSS + + V ++TP+K K KKKK+ +
Sbjct: 441 LSTSSVEETAAAAVSEETPSKDKKKKKKKHKGD 473
>Z410_MOUSE (Q8BKX7) Zinc finger protein 410 (Zinc finger protein
APA-1) (Another partner for ARF 1)
Length = 478
Score = 29.6 bits (65), Expect = 3.2
Identities = 20/90 (22%), Positives = 41/90 (45%), Gaps = 1/90 (1%)
Query: 1 MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNK-PIKVVYISNPMKVKTSASE 59
++S + T S++ + ++L P + N+ N+ + P V S + V E
Sbjct: 29 VESEAKDITCLSLLPVTEASECSRLMLPDETPNHANSSKEVPSSAVLRSLQVNVGPDGEE 88
Query: 60 FRALVQELTGQYAESPPNPSRFQEFDVNDS 89
RA + + ++ +P +PS Q+ +DS
Sbjct: 89 TRAQTVQKSPEFLTTPESPSLLQDLQPSDS 118
>POLG_HRV1B (P12916) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core
protein P2A); Core protein P2B; Core protein P2C; Core
protein P3A; Genome
Length = 2157
Score = 29.6 bits (65), Expect = 3.2
Identities = 22/94 (23%), Positives = 39/94 (41%), Gaps = 5/94 (5%)
Query: 39 NKPIKVVYISNPMKVKTSASEFRALVQELTGQYAESPPNPSRFQEFD-----VNDSGTDQ 93
++P+ V+ P K+ + F A + PP+P F +D + D
Sbjct: 1209 SEPVAVMIHGPPGTGKSITTNFLARMITNESDVYSLPPDPKYFDGYDNQSVVIMDDIMQN 1268
Query: 94 GGCENMMDCDKSDQTVVGVPSLVDPDDKGKPSEA 127
E+M + +V +P + D DKGKP ++
Sbjct: 1269 PDGEDMTLFCQMVSSVTFIPPMADLPDKGKPFDS 1302
>UVRB_MYCGE (P47319) UvrABC system protein B (UvrB protein)
(Excinuclease ABC subunit B)
Length = 656
Score = 29.3 bits (64), Expect = 4.2
Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 14/89 (15%)
Query: 1 MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEF 60
+D T + + KTP + KP T+ +PIK +K K++A +
Sbjct: 568 IDETKRRRTVQMEYNKLHNKTPKTVVKPL-------TFVQPIK-------LKAKSNAEKN 613
Query: 61 RALVQELTGQYAESPPNPSRFQEFDVNDS 89
AL+++LT + ++ N + ++ DS
Sbjct: 614 AALIKQLTKEMKKAAANQNYELAIEIRDS 642
>RGP1_MOUSE (P46061) Ran GTPase-activating protein 1
Length = 589
Score = 29.3 bits (64), Expect = 4.2
Identities = 19/86 (22%), Positives = 38/86 (44%), Gaps = 2/86 (2%)
Query: 52 KVKTSASEFRALVQELTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVG 111
K+K F + ++ AE+ + + ++ D+N + + GCE + + S
Sbjct: 294 KLKELNLSFCEIKRDAALVVAEAVADKAELEKLDLNGNALGEEGCEQLQEVMDSFNMAKV 353
Query: 112 VPSLVDPDDKGKPSEAGSSNESFDED 137
+ SL DD+G+ + E DE+
Sbjct: 354 LASL--SDDEGEDEDEEEEGEEDDEE 377
>PAC2_XENLA (Q9DDA9) Protein kinase C and casein kinase substrate in
neurons protein 2 (x-PACSIN2)
Length = 477
Score = 29.3 bits (64), Expect = 4.2
Identities = 23/81 (28%), Positives = 36/81 (44%), Gaps = 9/81 (11%)
Query: 67 LTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDDKGKP-- 124
L+ Q A+S NP +E V+ + T+ EN+ +K+ P+ D+ P
Sbjct: 346 LSVQSAQSTNNPFEDEEETVSINETENKKIENVGSYEKTH------PAEWSDDESNNPFN 399
Query: 125 -SEAGSSNESFDEDVLLMPEM 144
S+ N FDED L E+
Sbjct: 400 PSDTNGDNNPFDEDALTTLEV 420
>ARRI_ARATH (Q9FGT7) Two-component response regulator ARR18
Length = 635
Score = 29.3 bits (64), Expect = 4.2
Identities = 21/71 (29%), Positives = 37/71 (51%), Gaps = 4/71 (5%)
Query: 76 PNPSRFQEFDVNDSGTDQGGCENMMD-CDKSDQTVVGVPSLVDPDDKGKPSEAGSSNESF 134
P P+ F N+S +G E++M+ D S + V +++DP+ K E + ++ F
Sbjct: 545 PLPADNMVFRDNNSTRSKGLDESLMNPIDNSQEYVGKATTMLDPEMKSGKPENDNQHDVF 604
Query: 135 DEDVLLMPEMM 145
D+ +M EMM
Sbjct: 605 DD---IMNEMM 612
>SYM_CLOAB (Q97EW5) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 644
Score = 28.9 bits (63), Expect = 5.5
Identities = 13/29 (44%), Positives = 19/29 (64%), Gaps = 2/29 (6%)
Query: 132 ESFDEDV--LLMPEMMDNIWDLLPTSAFY 158
E DED+ L +PE +D+IWDL+ + Y
Sbjct: 396 EKLDEDIKKLKIPEALDHIWDLIKRANKY 424
>RGYR_SULSH (P74759) Reverse gyrase [Includes: Helicase (EC
3.6.1.-); Topoisomerase (EC 5.99.1.3)]
Length = 1166
Score = 28.9 bits (63), Expect = 5.5
Identities = 18/56 (32%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
Query: 5 STSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEF 60
+T+ TS S I+ + KT KL + +K++ N +N ++ + +P K KT A F
Sbjct: 545 NTNFTSLSNINLSEIKT--KLEESRKEEGNRVHFNISTGLLIVESPTKAKTIAKMF 598
>DMP1_RAT (P98193) Dentin matrix acidic phosphoprotein 1 precursor
(Dentin matrix protein-1) (DMP-1) (AG1)
Length = 489
Score = 28.9 bits (63), Expect = 5.5
Identities = 27/90 (30%), Positives = 37/90 (41%), Gaps = 17/90 (18%)
Query: 65 QELTGQYAESPPNP-SRFQEFDVNDSGTDQGGCENM------------MDCDK-SDQTVV 110
+E TG A+SPP P + D ++SG + G + MD DK D+
Sbjct: 31 EERTGNLAQSPPPPMANSDHTDSSESGEELGSDRSQYRPAGGLSKSAGMDADKEEDEDDS 90
Query: 111 GVPSLVDPDDKGKPSE---AGSSNESFDED 137
G + D D+ P E G S DED
Sbjct: 91 GDDTFGDEDNGPGPEERQWGGPSRLDSDED 120
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.306 0.125 0.346
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,338,093
Number of Sequences: 164201
Number of extensions: 873285
Number of successful extensions: 2276
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 2251
Number of HSP's gapped (non-prelim): 50
length of query: 165
length of database: 59,974,054
effective HSP length: 102
effective length of query: 63
effective length of database: 43,225,552
effective search space: 2723209776
effective search space used: 2723209776
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC122169.4