BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122165.8 - phase: 1 /pseudo
(144 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NEB1_HUMAN (Q9ULJ8) Neurabin-I (Neural tissue-specific F-actin b... 35 0.056
MV10_HUMAN (Q9HCE1) Potential helicase MOV-10 (EC 3.6.1.-) (Molo... 35 0.073
SNPH_HUMAN (O15079) Syntaphilin 34 0.095
LI49_CAEEL (Q20318) Protein lin-49 (Abnormal cell lineage protei... 34 0.095
SERI_BOMMO (P07856) Sericin precursor (Silk gum protein) 33 0.16
TRDN_HUMAN (Q13061) Triadin 33 0.21
MV10_MOUSE (P23249) Potential helicase MOV-10 (EC 3.6.1.-) (Molo... 33 0.28
KE72_HUMAN (Q9NX95) Protein KIAA1472 32 0.47
DNAK_METTH (O27351) Chaperone protein dnaK (Heat shock protein 7... 32 0.47
YRY5_CAEEL (Q09355) Hypothetical protein T15H9.5 in chromosome II 32 0.62
NOP5_RAT (Q9QZ86) Nucleolar protein NOP5 (Nucleolar protein 5) (... 32 0.62
SIB2_RAT (Q9QX72) SECIS-binding protein 2 (Selenocysteine insert... 31 0.80
SEN1_YEAST (Q00416) Helicase SEN1 (EC 3.6.1.-) (tRNA-splicing en... 31 0.80
LMD1_HUMAN (P29536) Leiomodin 1 (Leiomodin, muscle form) (64 kDa... 31 1.1
YOT2_CAEEL (P34648) Hypothetical protein ZK632.2 in chromosome III 30 1.4
YKY1_SCHPO (O13796) Hypothetical protein C1142.01 in chromosome I 30 1.8
YBS7_SCHPO (O94497) Hypothetical serine-rich protein C18E5.07 in... 30 1.8
T150_HUMAN (Q9Y2W1) Thyroid hormone receptor-associated protein ... 30 1.8
SSF2_HUMAN (P28290) Sperm-specific antigen 2 (Cleavage signal-1 ... 30 1.8
YE05_HAEIN (P44180) Hypothetical protein HI1405 30 2.3
>NEB1_HUMAN (Q9ULJ8) Neurabin-I (Neural tissue-specific F-actin
binding protein I) (Protein phosphatase 1 regulatory
subunit 9A)
Length = 1098
Score = 35.0 bits (79), Expect = 0.056
Identities = 30/104 (28%), Positives = 50/104 (47%), Gaps = 13/104 (12%)
Query: 28 IPSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRF 87
IP IQ S + +++ ++ ++P+S K EE P + PK + IPS +
Sbjct: 292 IPGEEIQQSKEPEDSTSNQQTPDSIDKDGPEE-----PCAESKAMPKSE--IPSPQ---- 340
Query: 88 KQKLKEADKRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPKD 131
Q L++A+ + GRE +EL GG+ TS + S K+
Sbjct: 341 SQLLEDAEANL--VGREAAKQQRKELAGGDFTSPDASASSCGKE 382
>MV10_HUMAN (Q9HCE1) Potential helicase MOV-10 (EC 3.6.1.-) (Moloney
leukemia virus 10 protein)
Length = 1003
Score = 34.7 bits (78), Expect = 0.073
Identities = 27/104 (25%), Positives = 47/104 (44%), Gaps = 7/104 (6%)
Query: 25 GFVIPSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGP---HGAPPSQPKQQEVIPS 81
GF I G+ D+ + N+ S +P AA K+ L P G P+ VI
Sbjct: 773 GFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKLLLAPSSKKGKARLSPRSVGVISP 832
Query: 82 NRKQ--RFKQKLKEADKRISGTG--RENKVDNLRELVGGEKTSV 121
RKQ + + + + D+ + G ++ KV ++ E G E++ +
Sbjct: 833 YRKQVEKIRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVI 876
>SNPH_HUMAN (O15079) Syntaphilin
Length = 538
Score = 34.3 bits (77), Expect = 0.095
Identities = 30/124 (24%), Positives = 56/124 (44%), Gaps = 25/124 (20%)
Query: 29 PSLGIQD-----SDQSKNNAASVESPNSAAKTKKEEKIYL--GPHGAPPSQPKQQEVIPS 81
P + ++D S S +N+ S + +S+ ++ K L HG P P+Q + P
Sbjct: 65 PPVSVRDAYGTSSLSSSSNSGSYKGSDSSPTPRRSMKYTLCSDNHGIKPPTPEQY-LTPL 123
Query: 82 NRKQ----RFKQKLKEADKRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPKDWLDPHC 137
+K+ K +LK+ R+ R+ ++D+L KT + + +DW++ C
Sbjct: 124 QQKEVCIRHLKARLKDTQDRLQD--RDTEIDDL-------KTQLSRMQ----EDWIEEEC 170
Query: 138 HEAE 141
H E
Sbjct: 171 HRVE 174
>LI49_CAEEL (Q20318) Protein lin-49 (Abnormal cell lineage protein
49)
Length = 1042
Score = 34.3 bits (77), Expect = 0.095
Identities = 30/102 (29%), Positives = 47/102 (45%), Gaps = 14/102 (13%)
Query: 29 PSLGIQ--DSDQSKNNAASVESPNSAAK---------TKKEEKIYLGPHGAPPSQPKQQE 77
P L +Q D+D SK+++A E P S+ K TK EE + P + +
Sbjct: 670 PFLDVQELDTDDSKDSSALSEIPGSSKKSSRKRGIQDTKMEEDEEIKPSTSGTNAVASVP 729
Query: 78 VIPSNRKQRFKQKLKEAD---KRISGTGRENKVDNLRELVGG 116
++ S+R+ + K K +AD + S G + NLR V G
Sbjct: 730 LLSSSRESKNKSKSSDADVSSPKSSWLGSPSTSQNLRRRVQG 771
>SERI_BOMMO (P07856) Sericin precursor (Silk gum protein)
Length = 389
Score = 33.5 bits (75), Expect = 0.16
Identities = 30/115 (26%), Positives = 45/115 (39%), Gaps = 16/115 (13%)
Query: 8 FVLKCMFFGLLLVVDTDGFVIPSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHG 67
FVLKC L L V G G +D+ + KN + S S+ K + I G H
Sbjct: 3 FVLKCTLIALALSVKAFGH---HPGNRDTVEVKNRKYNAASSESSYLNKDNDSISAGAHR 59
Query: 68 APPSQPKQQEVIPSNRKQRFKQKLKEADKRISGTGRENKVDNLRELV--GGEKTS 120
A + Q K K + +S +GR + ++ + GG K+S
Sbjct: 60 AKSVEQSQD-----------KSKYTSGPEGVSTSGRSQNYKDSKQAIISGGTKSS 103
>TRDN_HUMAN (Q13061) Triadin
Length = 728
Score = 33.1 bits (74), Expect = 0.21
Identities = 25/87 (28%), Positives = 41/87 (46%), Gaps = 13/87 (14%)
Query: 34 QDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKE 93
+DS ++K A VE P + KKE+ H P PK++ +PS++ + K K +
Sbjct: 377 EDSKKTKK-PAEVEQPKGKKQEKKEK------HVEPAKSPKKEHSVPSDK--QVKAKTER 427
Query: 94 ADKRISGTGRENKVDNLRELVGGEKTS 120
A + I + V +E EKT+
Sbjct: 428 AKEEIGAVSSKKAVPGKKE----EKTT 450
>MV10_MOUSE (P23249) Potential helicase MOV-10 (EC 3.6.1.-) (Moloney
leukemia virus 10 protein)
Length = 1004
Score = 32.7 bits (73), Expect = 0.28
Identities = 27/104 (25%), Positives = 46/104 (43%), Gaps = 7/104 (6%)
Query: 25 GFVIPSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGP---HGAPPSQPKQQEVIPS 81
GF I G+ D+ + N+ S +P AA K L P G P+ VI
Sbjct: 774 GFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKQLLAPSSKKGKARLSPRNVGVISP 833
Query: 82 NRKQ--RFKQKLKEADKRISGTG--RENKVDNLRELVGGEKTSV 121
RKQ + + + + D+ + G ++ KV ++ E G E++ +
Sbjct: 834 YRKQVEKIRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVI 877
>KE72_HUMAN (Q9NX95) Protein KIAA1472
Length = 595
Score = 32.0 bits (71), Expect = 0.47
Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 18/80 (22%)
Query: 66 HGAPPSQPKQQEVIPSNRKQ----RFKQKLKEADKRISGTGRENKVDNLRELVGGEKTSV 121
HG P P+Q + P +K+ K KLKE+++R+ RE+++ L+ + +
Sbjct: 189 HGVRPPNPEQY-LTPLQQKEVTVRHLKTKLKESERRLHE--RESEIVELKSQLARMR--- 242
Query: 122 GMAKGSSPKDWLDPHCHEAE 141
+DW++ CH E
Sbjct: 243 --------EDWIEEECHRVE 254
>DNAK_METTH (O27351) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 596
Score = 32.0 bits (71), Expect = 0.47
Identities = 24/103 (23%), Positives = 46/103 (44%), Gaps = 7/103 (6%)
Query: 22 DTDGFVIPSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPS 81
D D I ++ +D K A ++ +PN ++ + ++KI A + KQ+E+
Sbjct: 456 DIDANGILNVSAKDLGTGKEQAITITAPNKLSEEEIKQKIEEAKKHAEEDRRKQEEIEIR 515
Query: 82 NRKQRF----KQKLKEADKRISGTGR---ENKVDNLRELVGGE 117
N ++ L E ++ + E +V LREL+ G+
Sbjct: 516 NNADSMIYTAEKTLDELGDKVPAEKKEEVEKQVRELRELIAGD 558
>YRY5_CAEEL (Q09355) Hypothetical protein T15H9.5 in chromosome II
Length = 173
Score = 31.6 bits (70), Expect = 0.62
Identities = 35/121 (28%), Positives = 49/121 (39%), Gaps = 17/121 (14%)
Query: 29 PSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFK 88
PS D SKN SA K+ +E K P S K++ I +++ K
Sbjct: 36 PSQMSIDESHSKNQKLP-----SAEKSVEECK-------KPVSPEKKKSPIKVLSEKKLK 83
Query: 89 QKLKEADKRIS-----GTGRENKVDNLRELVGGEKTSVGMAKGSSPKDWLDPHCHEAEFE 143
K KE DK +E K DN LV K + + +PK L+ H EAE +
Sbjct: 84 SKKKEEDKEPDEKVEKDVKKEVKADNNEPLVKNLKIAKKEQEEENPKTDLESHKDEAEAK 143
Query: 144 R 144
+
Sbjct: 144 K 144
>NOP5_RAT (Q9QZ86) Nucleolar protein NOP5 (Nucleolar protein 5)
(Nopp140 associated protein)
Length = 534
Score = 31.6 bits (70), Expect = 0.62
Identities = 14/62 (22%), Positives = 31/62 (49%)
Query: 34 QDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKE 93
++ ++++ E P K KK++K ++ +P +PS K++ K+K K+
Sbjct: 472 EEMEEAEEEQVVEEEPTVKKKKKKDKKKHIKEEPLSEEEPCTSTAVPSPEKKKKKKKKKD 531
Query: 94 AD 95
A+
Sbjct: 532 AE 533
>SIB2_RAT (Q9QX72) SECIS-binding protein 2 (Selenocysteine insertion
sequence binding protein 2)
Length = 846
Score = 31.2 bits (69), Expect = 0.80
Identities = 21/95 (22%), Positives = 45/95 (47%), Gaps = 1/95 (1%)
Query: 36 SDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKEAD 95
S +KN + + E+ +S +E++++ GP A SQ + E S++K + K++ ++
Sbjct: 324 SADAKNVSVTSEALSSDPSFSREKRVHPGPK-AKASQGSELEQNESSKKNKKKKEKSKSS 382
Query: 96 KRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPK 130
+ ++++ E S +G SPK
Sbjct: 383 YEVLPVQEPPRIEDAEEFPNLSVASERRHRGESPK 417
>SEN1_YEAST (Q00416) Helicase SEN1 (EC 3.6.1.-) (tRNA-splicing
endonuclease positive effector)
Length = 2231
Score = 31.2 bits (69), Expect = 0.80
Identities = 18/63 (28%), Positives = 30/63 (47%)
Query: 36 SDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKEAD 95
+D K N ESP++++ TKK+ I+ G PK + SN+K K+ +
Sbjct: 1939 ADDKKKNNKKAESPSTSSGTKKKSSIFGGMSVPSAVVPKTFPDVDSNKKAAAVVGKKKNN 1998
Query: 96 KRI 98
K +
Sbjct: 1999 KHV 2001
>LMD1_HUMAN (P29536) Leiomodin 1 (Leiomodin, muscle form) (64 kDa
autoantigen D1) (64 kDa autoantigen 1D) (64 kDa
autoantigen 1D3) (Thyroid-associated ophthalmopathy
autoantigen) (Smooth muscle leiomodin) (SM-Lmod)
Length = 572
Score = 30.8 bits (68), Expect = 1.1
Identities = 26/116 (22%), Positives = 52/116 (44%), Gaps = 6/116 (5%)
Query: 20 VVDTDGFVIPSLGIQDSDQSKNNAASVESPNSAAK--TKKEEKIYLGPHGAPPSQPKQQE 77
VVD DG V +G++ +Q++ + V + + K+ +K+ S+ + +
Sbjct: 10 VVDPDGSV--PVGLRQRNQTEKQSTGVYNREAMLNFCEKETKKLMQREMSMDESKQVETK 67
Query: 78 VIPSNRKQRFKQKLKEA--DKRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPKD 131
N ++R + K+A +R S G+E K L++ ++ G G PK+
Sbjct: 68 TDAKNGQERGRDASKKALGPRRNSDLGKEPKRGGLKKSFSRDRDEAGGKSGEKPKE 123
>YOT2_CAEEL (P34648) Hypothetical protein ZK632.2 in chromosome III
Length = 710
Score = 30.4 bits (67), Expect = 1.4
Identities = 24/111 (21%), Positives = 46/111 (40%), Gaps = 4/111 (3%)
Query: 35 DSDQSKNNAASVESPNSAAKTKKEE---KIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKL 91
DS + KN+ +V+ P+S EE K G ++E+ + KQKL
Sbjct: 586 DSSEIKNSLPAVDEPSSVKDEVSEETPQKEAFGSKVQKRVAQWEEELEAEKEELAKKQKL 645
Query: 92 KEADKRISGTGRENKVDNLRELVGGEKTSVGMA-KGSSPKDWLDPHCHEAE 141
+ ++ R + D +++ G E G+ + W+ P+ ++E
Sbjct: 646 EAEEEAKKKVQRVRRRDIEKKIAGSEDYGAGVEDRDEKYSTWMPPNADQSE 696
>YKY1_SCHPO (O13796) Hypothetical protein C1142.01 in chromosome I
Length = 667
Score = 30.0 bits (66), Expect = 1.8
Identities = 17/72 (23%), Positives = 39/72 (53%), Gaps = 11/72 (15%)
Query: 38 QSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKEADKR 97
+ +NNA + E+ S ++ +++E + G + ++ +N+K + K+K K+ K+
Sbjct: 59 EKENNAINSEAEKSVSEEEQDEPLVEG----------ESPIVSTNKKAKNKKKKKKQQKK 108
Query: 98 ISGTGRENKVDN 109
TG+ + +DN
Sbjct: 109 KKVTGKRD-LDN 119
>YBS7_SCHPO (O94497) Hypothetical serine-rich protein C18E5.07 in
chromosome II
Length = 615
Score = 30.0 bits (66), Expect = 1.8
Identities = 23/94 (24%), Positives = 43/94 (45%), Gaps = 7/94 (7%)
Query: 44 ASVESPN-SAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKEADKRISGTG 102
++++ PN +A ++ + +K + GP + SQP S+ K+ + K I +
Sbjct: 334 SNLDIPNENAHESSRSKKKHTGPSLSSASQPSAASSSSSSEPSNL-DKINDVKKNIEVSA 392
Query: 103 REN-----KVDNLRELVGGEKTSVGMAKGSSPKD 131
E K D + VGGE + + S+PK+
Sbjct: 393 NEPQPRPVKEDVPKSQVGGETDTTDVINNSTPKE 426
>T150_HUMAN (Q9Y2W1) Thyroid hormone receptor-associated protein
complex 150 kDa component (Trap150) (Thyroid hormone
receptor associated protein 3)
Length = 955
Score = 30.0 bits (66), Expect = 1.8
Identities = 25/106 (23%), Positives = 36/106 (33%)
Query: 29 PSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFK 88
P G S K + S S + + + K + S V S RK +
Sbjct: 120 PRRGRSRSRSPKRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKE 179
Query: 89 QKLKEADKRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPKDWLD 134
+K D R S +N+ D ++E TS S K W D
Sbjct: 180 KKSSSKDSRPSQAAGDNQGDEVKEQTFSGGTSQDTKASESSKPWPD 225
>SSF2_HUMAN (P28290) Sperm-specific antigen 2 (Cleavage signal-1
protein) (CS-1)
Length = 267
Score = 30.0 bits (66), Expect = 1.8
Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 7/65 (10%)
Query: 66 HGAPPSQPKQQEVIPSNRKQRFKQKLKEADKRISGTGRENKVDNLRELVGGEKTSVGMAK 125
HG PS P +E+ + + +Q ++E + I G R RE+V G +V +K
Sbjct: 206 HGKLPSMPAAEEMHKNVEQDELQQVIREIKESIVGEIR-------REIVSGLLAAVSSSK 258
Query: 126 GSSPK 130
S+ K
Sbjct: 259 ASNSK 263
>YE05_HAEIN (P44180) Hypothetical protein HI1405
Length = 366
Score = 29.6 bits (65), Expect = 2.3
Identities = 21/75 (28%), Positives = 37/75 (49%), Gaps = 9/75 (12%)
Query: 30 SLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQ 89
+ GI+ SD+ K E S ++ KE P G P++PK+++ P N+K+ +
Sbjct: 190 AFGIKLSDEQKKQIEDEEK--SGSEEGKE------PKGEQPTKPKEKKSEPENKKEDDVE 241
Query: 90 KLKEADKRISGTGRE 104
K +E +K + E
Sbjct: 242 K-EELEKSLKAKDEE 255
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,824,913
Number of Sequences: 164201
Number of extensions: 742406
Number of successful extensions: 2806
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 45
Number of HSP's that attempted gapping in prelim test: 2771
Number of HSP's gapped (non-prelim): 77
length of query: 144
length of database: 59,974,054
effective HSP length: 99
effective length of query: 45
effective length of database: 43,718,155
effective search space: 1967316975
effective search space used: 1967316975
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122165.8