BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122165.12 + phase: 1 /pseudo
(150 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YCXM_PORPU (P51360) Hypothetical 23.3 kDa protein in RPS6-THIG i... 64 2e-10
DNAB_ECOLI (P03005) Replicative DNA helicase (EC 3.6.1.-) 32 0.68
YSPK_CAEEL (Q19425) Hypothetical protein F13H10.3 in chromosome IV 29 3.4
OLF6_CHICK (P37072) Olfactory receptor-like protein COR6 29 4.4
DNAB_SALTY (P10338) Replicative DNA helicase (EC 3.6.1.-) 29 4.4
TDH_XANAC (Q8PNN2) L-threonine 3-dehydrogenase (EC 1.1.1.103) 28 5.8
OPSD_CHICK (P22328) Rhodopsin 28 5.8
CYB6_PORPU (P51341) Cytochrome b6 28 7.5
CINA_ELEEL (P02719) Sodium channel protein (Na(+) channel) 28 7.5
CHS2_SCHPO (O74756) Chitin synthase-like protein 2 28 7.5
RF3_GLOVI (Q7NGL0) Peptide chain release factor 3 (RF-3) 28 9.8
CYB6_CHLRE (Q00471) Cytochrome b6 28 9.8
>YCXM_PORPU (P51360) Hypothetical 23.3 kDa protein in RPS6-THIG
intergenic region (ORF203)
Length = 203
Score = 63.5 bits (153), Expect = 2e-10
Identities = 44/157 (28%), Positives = 86/157 (54%), Gaps = 11/157 (7%)
Query: 2 SDRLISALCYFYPFFDGIQ-YGKFVITQFYPIQAI---VQPLIPAIRVFKSFPFNGFLVF 57
S RL+S + Y+ P F+G+Q +G++V+ YP+ AI + L+P + + + G + F
Sbjct: 45 SIRLVSIIPYYLPLFEGLQNFGQYVLPD-YPVGAIPLYKKILLPMLIFYMNHAILGLVTF 103
Query: 58 LTLYFFVVRNPN---FSKYVRFNTMQAIVLDVLLIFPDLLERGFNPRGGFGLDLL-MSLD 113
LY+ +VRN + + VRFN+MQ+I+L ++ + R F F + + +++
Sbjct: 104 FALYYVLVRNKSPITVHQLVRFNSMQSILLFLVGSLFGAIFRAFPIE--FRISFIGLTVC 161
Query: 114 STVFLFLLVCLIYGSSSCILGQLPRLPIVADAADRQV 150
+ +F F+L + Y I G+ +P++++A Q+
Sbjct: 162 NMMFWFILSTITYSIVKAIQGKYSNIPVISEAVRIQI 198
>DNAB_ECOLI (P03005) Replicative DNA helicase (EC 3.6.1.-)
Length = 471
Score = 31.6 bits (70), Expect = 0.68
Identities = 16/49 (32%), Positives = 27/49 (54%)
Query: 69 NFSKYVRFNTMQAIVLDVLLIFPDLLERGFNPRGGFGLDLLMSLDSTVF 117
N S Y +A+V +++ + ++ E GF+P+G DLL +S VF
Sbjct: 118 NISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVF 166
>YSPK_CAEEL (Q19425) Hypothetical protein F13H10.3 in chromosome IV
Length = 615
Score = 29.3 bits (64), Expect = 3.4
Identities = 26/87 (29%), Positives = 40/87 (45%), Gaps = 7/87 (8%)
Query: 55 LVFLTLYFFVVRNPNFSKYVRFN-----TMQAIVLDVLLIFPDLLERGFNPRGGFGLDLL 109
+ L L F+VR+ F Y F ++ I+L+VLLI + F P G L +
Sbjct: 506 ITVLPLLMFLVRSQLF--YAIFGQTWPGAIRVIILNVLLIAVAVGFATFYPNVGSILRYV 563
Query: 110 MSLDSTVFLFLLVCLIYGSSSCILGQL 136
S+ V++F L ++Y S G L
Sbjct: 564 GSISGLVYVFALPAMVYIKQSEAAGTL 590
>OLF6_CHICK (P37072) Olfactory receptor-like protein COR6
Length = 312
Score = 28.9 bits (63), Expect = 4.4
Identities = 24/73 (32%), Positives = 36/73 (48%), Gaps = 3/73 (4%)
Query: 56 VFLTLYFFVVRNPNFSKYVRFNTMQAIVLDVLLIFPDLLERGFNPRGGFGL--DLLMSLD 113
VF+ L +R+ KY F+T + ++ V L ++ F P F L D +MSL
Sbjct: 219 VFIILTVVRIRSKE-RKYKAFSTCTSHLMAVSLFHGTIVFMYFQPANNFSLDKDKIMSLF 277
Query: 114 STVFLFLLVCLIY 126
TV + +L LIY
Sbjct: 278 YTVVIPMLNPLIY 290
>DNAB_SALTY (P10338) Replicative DNA helicase (EC 3.6.1.-)
Length = 471
Score = 28.9 bits (63), Expect = 4.4
Identities = 14/49 (28%), Positives = 27/49 (54%)
Query: 69 NFSKYVRFNTMQAIVLDVLLIFPDLLERGFNPRGGFGLDLLMSLDSTVF 117
N S Y +A+V D++ + ++ + G++P+G +LL +S VF
Sbjct: 118 NISAYADIVRERAVVRDMIAVAHEIADAGYDPQGRNSDELLDLAESRVF 166
>TDH_XANAC (Q8PNN2) L-threonine 3-dehydrogenase (EC 1.1.1.103)
Length = 340
Score = 28.5 bits (62), Expect = 5.8
Identities = 14/36 (38%), Positives = 21/36 (57%)
Query: 103 GFGLDLLMSLDSTVFLFLLVCLIYGSSSCILGQLPR 138
GF + L MS +S F +L C+ +G +LG +PR
Sbjct: 231 GFDVGLEMSGNSRAFNDMLDCMYHGGKIAMLGIMPR 266
>OPSD_CHICK (P22328) Rhodopsin
Length = 351
Score = 28.5 bits (62), Expect = 5.8
Identities = 24/131 (18%), Positives = 55/131 (41%), Gaps = 18/131 (13%)
Query: 20 QYGKFVITQFYPIQAIVQPLIPAIRVFKSFPFNGFLVFLTLYFFVVRNPNFSKYVRFNTM 79
+Y ++ + + + A+ + I + FP N +++T+ +R P + + +
Sbjct: 25 EYPQYYLAEPWKFSALAAYMFMLILL--GFPVNFLTLYVTIQHKKLRTP-----LNYILL 77
Query: 80 QAIVLDVLLIFPDLLERGFNPRGGFGLDLLMSLDSTVFLFLLVCLIYGSSSCILGQLPRL 139
+V D+ ++F GGF + S++ + C I G + + G++
Sbjct: 78 NLVVADLFMVF-----------GGFTTTMYTSMNGYFVFGVTGCYIEGFFATLGGEIALW 126
Query: 140 PIVADAADRQV 150
+V A +R V
Sbjct: 127 SLVVLAVERYV 137
>CYB6_PORPU (P51341) Cytochrome b6
Length = 215
Score = 28.1 bits (61), Expect = 7.5
Identities = 14/54 (25%), Positives = 25/54 (45%), Gaps = 13/54 (24%)
Query: 18 GIQYGKFVITQFYPIQAIVQPLIPAIRVFKSFPFNGFLVFLTLYFFVVRNPNFS 71
G+ G+ +T+FY + V PL+ A VF+ ++F ++R S
Sbjct: 172 GVSVGQGTLTRFYSLHTFVLPLLTA-------------VFMLMHFLMIRKQGIS 212
>CINA_ELEEL (P02719) Sodium channel protein (Na(+) channel)
Length = 1820
Score = 28.1 bits (61), Expect = 7.5
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 13/79 (16%)
Query: 24 FVITQFYPIQAIVQPLIPAIRVFKSFPFNGFL---VFLTLYFFVVRN-PNFSKYVRFNTM 79
++ + F PI+ AIRVF + FN F+ +F F + N P +SK V +
Sbjct: 103 YIFSPFNPIRR------GAIRVFVNSAFNFFIMFTIFSNCIFMTISNPPAWSKIVEYTFT 156
Query: 80 QAIVLDVLLIFPDLLERGF 98
+V++ +L RGF
Sbjct: 157 GIYTFEVIV---KVLSRGF 172
>CHS2_SCHPO (O74756) Chitin synthase-like protein 2
Length = 926
Score = 28.1 bits (61), Expect = 7.5
Identities = 18/67 (26%), Positives = 27/67 (39%), Gaps = 5/67 (7%)
Query: 72 KYVRFNTMQAIVLDVLLIFPDLLERGFNPRGGFGLDLLMSLDSTVFLFLLVCLIYGSSSC 131
K V F +M L + NP G + L + VF ++L+CLI+
Sbjct: 605 KLVNFFSMANFFLAFYFVCNATSYSSLNPYGNWARPLFL-----VFEYILICLIFSQFML 659
Query: 132 ILGQLPR 138
+G PR
Sbjct: 660 AMGNRPR 666
>RF3_GLOVI (Q7NGL0) Peptide chain release factor 3 (RF-3)
Length = 531
Score = 27.7 bits (60), Expect = 9.8
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 47 KSFPFNGFLVFLTLYFFVVRNPNFSKYVRF 76
K F G +F +F +RNPN SKY +F
Sbjct: 383 KPLAFEGIPLFPPEHFATLRNPNPSKYKQF 412
>CYB6_CHLRE (Q00471) Cytochrome b6
Length = 215
Score = 27.7 bits (60), Expect = 9.8
Identities = 14/54 (25%), Positives = 25/54 (45%), Gaps = 13/54 (24%)
Query: 18 GIQYGKFVITQFYPIQAIVQPLIPAIRVFKSFPFNGFLVFLTLYFFVVRNPNFS 71
G+ G+ +T+FY + V PL+ A VF+ ++F ++R S
Sbjct: 172 GVGVGQATLTRFYSLHTFVLPLLTA-------------VFMLMHFLMIRKQGIS 212
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.335 0.153 0.470
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,562,164
Number of Sequences: 164201
Number of extensions: 705911
Number of successful extensions: 2391
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2383
Number of HSP's gapped (non-prelim): 12
length of query: 150
length of database: 59,974,054
effective HSP length: 100
effective length of query: 50
effective length of database: 43,553,954
effective search space: 2177697700
effective search space used: 2177697700
T: 11
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122165.12