BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.14 - phase: 0
(420 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310... 68 4e-11
LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog 54 6e-07
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog 49 2e-05
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ... 49 2e-05
YAFA_SHIFL (Q83M81) Hypothetical UPF0255 protein yafA 34 0.80
YAFA_ECOL6 (Q8FKM5) Hypothetical UPF0255 protein yafA 34 0.80
YAFA_ECO57 (Q8X7N7) Hypothetical UPF0255 protein yafA 34 0.80
YAFA_ECOLI (P04335) Hypothetical UPF0255 protein yafA 33 1.0
YKG4_CAEEL (P46554) Hypothetical protein B0285.4 in chromosome III 32 2.3
DPP4_RAT (P14740) Dipeptidyl peptidase IV (EC 3.4.14.5) (DPP IV)... 32 3.0
SYM_BIFLO (P59076) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 30 8.9
DPP4_MOUSE (P28843) Dipeptidyl peptidase IV (EC 3.4.14.5) (DPP I... 30 8.9
CATB_AJECA (Q9Y7C2) Catalase B (EC 1.11.1.6) 30 8.9
CATA_ERYGR (Q8X1P0) Catalase (EC 1.11.1.6) 30 8.9
>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
(ORF154)
Length = 154
Score = 68.2 bits (165), Expect = 4e-11
Identities = 38/151 (25%), Positives = 72/151 (47%), Gaps = 10/151 (6%)
Query: 144 SIPIFYLSFLKMPNKVWRKIVKIQCDFLWGGARGGKKLCWVKWRVVCQPR-SKGGLGVRD 202
++P++ +S ++ + +K+ +F W +K+ WV W+ +C+ + GGLG RD
Sbjct: 2 ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61
Query: 203 IRVVNLSLLAKWRWRVLQGEEGLWKEVLIEKYGTRVCNLLVEDDGSWPSYISRWWKDVAF 262
+ N +LLAK +R++ L +L +Y +++ G+ PSY W+ +
Sbjct: 62 LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPH-SSMMECSVGTRPSYA---WRSIIH 117
Query: 263 LEDEGGERWFNAEVVRKVGCGNSTSFWKDPW 293
G + ++R +G G T W D W
Sbjct: 118 -----GRELLSRGLLRTIGDGIHTKVWLDRW 143
>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
Length = 1259
Score = 54.3 bits (129), Expect = 6e-07
Identities = 63/266 (23%), Positives = 107/266 (39%), Gaps = 33/266 (12%)
Query: 11 KGFKIKNDGTGISHLQYADDTLCIGEASVDNLWTLKALLRGFEMASGLKVNFYKS--CLM 68
KG ++ + +S +ADD + E + + L L+ F SG K+N KS L
Sbjct: 685 KGIQLGKEEVKLS--LFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLY 742
Query: 69 GINVPSEFMTMA-CDFLNCSEGVVPFKYLGLPVGANSFKLV--TWEPLLEQLSRKLHSWG 125
N +E M+ F S+ + KYLG+ + + L ++PLL ++ + W
Sbjct: 743 TNNRQTESQIMSELPFTIASKRI---KYLGIQLTRDVKDLFKENYKPLLNEIKEDTNKWK 799
Query: 126 NKYVSLGGRIVLLNAVI--------NSIPIFYLSFLKMPNKVWRKIVKIQCDFLWGGARG 177
N S GRI ++ I N+IPI K+P + ++ K F+W
Sbjct: 800 NIPCSWVGRINIVKMAILPKVIYRFNAIPI------KLPMTFFTELEKTTLKFIW----- 848
Query: 178 GKKLCWVKWRVVCQPRSKGGLGVRDIRVVNLSLLAKWRWRVLQGEE-GLWKEVLIEKYGT 236
+K + + Q GG+ + D ++ + + K W Q + W +
Sbjct: 849 NQKRAHIAKSTLSQKNKAGGITLPDFKLYYKATVTKTAWYWYQNRDIDQWNRTEPSEIMP 908
Query: 237 RVCNLLVEDDGSWPSYISRWWKDVAF 262
+ N L+ D P +W KD F
Sbjct: 909 HIYNYLIFDK---PEKNKQWGKDSLF 931
>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
Length = 1260
Score = 49.3 bits (116), Expect = 2e-05
Identities = 57/258 (22%), Positives = 107/258 (41%), Gaps = 17/258 (6%)
Query: 11 KGFKIKNDGTGISHLQYADDTLCIGEASVDNLWTLKALLRGFEMASGLKVNFYKSCLMGI 70
KG I ++ +S +ADD + E + D+ L +++ + SG K+N +KS
Sbjct: 685 KGIHIGSEEIKLS--LFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIY 742
Query: 71 NVPSEFMTMACDFLNCSEGVVPFKYLG--LPVGANSFKLVTWEPLLEQLSRKLHSWGNKY 128
++ D + + KYLG L +E L ++++ ++ W N
Sbjct: 743 TNNNQAEKTVKDSIPFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKEIAEDVNKWKNIP 802
Query: 129 VSLGGR--IVLLNAVINSIPIFYLSFLKMPNKVWRKIVKIQCDFLWGGARGGKKLCWVKW 186
S GR IV ++ + +I F +K P ++ + KI F+W +K +
Sbjct: 803 CSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIW-----NQKKPQIAK 857
Query: 187 RVVCQPRSKGGLGVRDIRVVNLSLLAK--WRWRVLQGEEGLWKEVLIEKYGTRVCNLLVE 244
++ GG+ + D+R+ S++ K W W E +W + ++ + L+
Sbjct: 858 TLLSNKNKAGGITLPDLRLYYKSIVIKTAWYWH-KNREVDVWNRIENQEMDPATYHYLIF 916
Query: 245 DDGSWPSYISRWWKDVAF 262
D P +W KD F
Sbjct: 917 DK---PIKNIQWGKDSLF 931
>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein
(ORF 2)
Length = 1308
Score = 48.9 bits (115), Expect = 2e-05
Identities = 52/202 (25%), Positives = 89/202 (43%), Gaps = 13/202 (6%)
Query: 7 RNLFKGFKIKNDGTGISHLQYADDTLCIGEASVDNLWTLKALLRGFEMASGLKVNFYKSC 66
R G +K + YADD + + + VD L + + AS ++N+ KS
Sbjct: 674 RKRLTGLVLKEPDMRVVLSAYADDVILVAQDLVD-LERAQECQEVYAAASSARINWSKSS 732
Query: 67 -LMGINVPSEFMTMACDFLNCSEGVVPFKYLGLPVGANSFKLV-TWEPLLEQLSRKLHSW 124
L+ ++ +F+ A ++ ++ KYLG+ + A + + + L E + +L W
Sbjct: 733 GLLEGSLKVDFLPPAFRDISWESKII--KYLGVYLSAEEYPVSQNFIELEECVLTRLGKW 790
Query: 125 GN--KYVSLGGRIVLLNAVINSIPIFYLSFLKMPNKVWRKIVKIQCDFLWGGARGGKKLC 182
K +S+ GR +++N ++ S + L L + KI + DFLW G
Sbjct: 791 KGFAKVLSMRGRALVINQLVASQIWYRLICLSPTQEFIAKIQRRLLDFLWIGKH------ 844
Query: 183 WVKWRVVCQPRSKGGLGVRDIR 204
WV V P +GG GV IR
Sbjct: 845 WVSAGVSSLPLKEGGQGVVCIR 866
>YAFA_SHIFL (Q83M81) Hypothetical UPF0255 protein yafA
Length = 414
Score = 33.9 bits (76), Expect = 0.80
Identities = 11/34 (32%), Positives = 17/34 (49%)
Query: 327 GGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVN 360
GG WI+EW VW+++ L G W++
Sbjct: 94 GGNWIYEWATQAMVWQQKACTEEDPQLSGRHWLH 127
>YAFA_ECOL6 (Q8FKM5) Hypothetical UPF0255 protein yafA
Length = 414
Score = 33.9 bits (76), Expect = 0.80
Identities = 11/34 (32%), Positives = 17/34 (49%)
Query: 327 GGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVN 360
GG WI+EW VW+++ L G W++
Sbjct: 94 GGNWIYEWATQAMVWQQKACAEEDPQLSGRHWLH 127
>YAFA_ECO57 (Q8X7N7) Hypothetical UPF0255 protein yafA
Length = 414
Score = 33.9 bits (76), Expect = 0.80
Identities = 11/34 (32%), Positives = 17/34 (49%)
Query: 327 GGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVN 360
GG WI+EW VW+++ L G W++
Sbjct: 94 GGNWIYEWATQAMVWQQKACAEEDPQLSGRHWLH 127
>YAFA_ECOLI (P04335) Hypothetical UPF0255 protein yafA
Length = 414
Score = 33.5 bits (75), Expect = 1.0
Identities = 11/34 (32%), Positives = 17/34 (49%)
Query: 327 GGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVN 360
GG WI+EW VW+++ L G W++
Sbjct: 94 GGNWIYEWATQAMVWQQKACAEDDPQLSGRHWLH 127
>YKG4_CAEEL (P46554) Hypothetical protein B0285.4 in chromosome III
Length = 333
Score = 32.3 bits (72), Expect = 2.3
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 7/73 (9%)
Query: 222 EEGLWKEVLIEKYGTRVCNLLVEDDGSWPSYISRWWKDVAFLE----DEGGERWFNAEVV 277
++G WK+ I ++ N V + +P +W A +E D E NA+VV
Sbjct: 47 QKGYWKDEFISRFANSSSN--VSEARRFPEISMGYWARTAAIEKYVRDFLNEFDGNAQVV 104
Query: 278 RKVGCGNSTSFWK 290
+GCG T FW+
Sbjct: 105 -SLGCGFDTLFWR 116
>DPP4_RAT (P14740) Dipeptidyl peptidase IV (EC 3.4.14.5) (DPP IV)
(T-cell activation antigen CD26) (GP110 glycoprotein)
(Bile canaliculus domain-specific membrane glycoprotein)
Length = 767
Score = 32.0 bits (71), Expect = 3.0
Identities = 27/120 (22%), Positives = 47/120 (38%), Gaps = 23/120 (19%)
Query: 311 NNKETLVEECRQMNGVGGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVNDPDRWVKSCY 370
N ++ + EE N W E H+ +VW+ ++ + ++ L HR +
Sbjct: 136 NKRQLITEEKIPNNTQWITWSQEGHKLAYVWKNDIYVKIEPHLPSHRITST--------- 186
Query: 371 GKLERLLGGEVEWSLEE--LRVLESIWNS------------KAPLKVIAFSWKLDESLLY 416
GK + G +W EE ++W S + +I +S+ DESL Y
Sbjct: 187 GKENVIFNGINDWVYEEEIFGAYSALWWSPNGTFLAYAQFNDTGVPLIEYSFYSDESLQY 246
>SYM_BIFLO (P59076) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 621
Score = 30.4 bits (67), Expect = 8.9
Identities = 12/37 (32%), Positives = 21/37 (56%)
Query: 329 GWIFEWHRPLFVWEEELLISLKEDLEGHRWVNDPDRW 365
GWI ++ L+VW + ++ L +E R DP++W
Sbjct: 243 GWIDNPNKKLYVWFDAVIGYLSASIEWARRQGDPEKW 279
>DPP4_MOUSE (P28843) Dipeptidyl peptidase IV (EC 3.4.14.5) (DPP IV)
(T-cell activation antigen CD26) (Thymocyte-activating
molecule) (THAM)
Length = 760
Score = 30.4 bits (67), Expect = 8.9
Identities = 26/120 (21%), Positives = 47/120 (38%), Gaps = 23/120 (19%)
Query: 311 NNKETLVEECRQMNGVGGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVNDPDRWVKSCY 370
N ++ + EE N W E H+ +VW+ ++ + ++ L HR +
Sbjct: 132 NKRQLITEEKIPNNTQWITWSPEGHKLAYVWKNDIYVKVEPHLPSHRITST--------- 182
Query: 371 GKLERLLGGEVEWSLEE--LRVLESIWNS------------KAPLKVIAFSWKLDESLLY 416
G+ + G +W EE ++W S + +I +S+ DESL Y
Sbjct: 183 GEENVIYNGITDWVYEEEVFGAYSALWWSPNNTFLAYAQFNDTGVPLIEYSFYSDESLQY 242
>CATB_AJECA (Q9Y7C2) Catalase B (EC 1.11.1.6)
Length = 728
Score = 30.4 bits (67), Expect = 8.9
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Query: 193 RSKGGLGVRDIRVVN---LSLLAKWRWRVLQGEEGL-WKE 228
R G GV R+V S L K+RW+ LQG GL W+E
Sbjct: 246 RHVDGWGVHTFRLVTDEGNSTLVKFRWKTLQGRAGLVWEE 285
>CATA_ERYGR (Q8X1P0) Catalase (EC 1.11.1.6)
Length = 718
Score = 30.4 bits (67), Expect = 8.9
Identities = 17/43 (39%), Positives = 22/43 (50%), Gaps = 4/43 (9%)
Query: 193 RSKGGLGVRDIRVVN---LSLLAKWRWRVLQGEEGL-WKEVLI 231
R G GV +R+V S L KW W+ QG+ L W+E I
Sbjct: 242 RHMDGFGVHTMRLVTDDGKSKLVKWHWKTKQGKASLVWEEAQI 284
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.141 0.472
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,800,474
Number of Sequences: 164201
Number of extensions: 2521437
Number of successful extensions: 4526
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 4514
Number of HSP's gapped (non-prelim): 18
length of query: 420
length of database: 59,974,054
effective HSP length: 113
effective length of query: 307
effective length of database: 41,419,341
effective search space: 12715737687
effective search space used: 12715737687
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 67 (30.4 bits)
Medicago: description of AC122162.14