BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121245.12 + phase: 0 /pseudo
(136 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RNHL_BACSU (P54162) 14.7 kDa ribonuclease H-like protein 42 4e-04
RNH_STRCO (Q9X7R6) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 37 0.016
YM53_MYCBO (P64956) Hypothetical protein Mb2253c 36 0.021
YM28_MYCTU (P64955) Hypothetical protein Rv2228c/MT2287 36 0.021
RNH_ECOLI (P00647) Ribonuclease HI (EC 3.1.26.4) (RNase HI) (Rib... 34 0.11
RNH_THETH (P29253) Ribonuclease H (EC 3.1.26.4) (RNase H) 33 0.23
RNH_HAEDU (Q7VM15) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 32 0.40
RNH_XANCP (Q8PBX8) Ribonuclease H (EC 3.1.26.4) (RNase H) 31 0.89
RNH_XANAC (Q8PNH8) Ribonuclease H (EC 3.1.26.4) (RNase H) 30 1.5
RNH_ZYMMO (O69014) Ribonuclease H (EC 3.1.26.4) (RNase H) 30 2.0
RNH_PHOLU (Q8GF77) Ribonuclease H (EC 3.1.26.4) (RNase H) 30 2.0
RNH_SALTY (P23329) Ribonuclease HI (EC 3.1.26.4) (RNase HI) (Rib... 29 2.6
RNH_HELPJ (Q9ZLH3) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 29 2.6
RNH_PASMU (P57813) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 29 3.4
METH_MYCTU (O33259) Methionine synthase (EC 2.1.1.13) (5-methylt... 29 3.4
Z276_HUMAN (Q8N554) Zinc finger protein 276 homolog (Zfp-276) 28 4.4
RNH_YERPE (Q8ZH30) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 28 4.4
RNH_NEIMB (Q9JYE5) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 28 4.4
RNH_NEIMA (Q9JTD9) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 28 4.4
RNH_HELPY (P56120) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 28 4.4
>RNHL_BACSU (P54162) 14.7 kDa ribonuclease H-like protein
Length = 132
Score = 42.0 bits (97), Expect = 4e-04
Identities = 25/72 (34%), Positives = 39/72 (53%), Gaps = 2/72 (2%)
Query: 28 KINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDLG 87
+I VDG+S GNP +G G +++ FS G TN AE A+ +G++L G
Sbjct: 4 EIYVDGASAGNPGPSGIGIFIKHE--GKAESFSIPIGVHTNQEAEFLALIEGMKLCATRG 61
Query: 88 YRSIIMESDSQV 99
Y+S+ +DS +
Sbjct: 62 YQSVSFRTDSDI 73
>RNH_STRCO (Q9X7R6) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 231
Score = 36.6 bits (83), Expect = 0.016
Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 12/77 (15%)
Query: 32 DGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDLGYRSI 91
DG+S GNP AG+ ++ + N + +G G+ATN AEL+A+ +
Sbjct: 10 DGASKGNPGPAGWAWVVADASENPVRWEAGPLGKATNNIAELTAL------------ERL 57
Query: 92 IMESDSQVALDLILDTK 108
+ +D V L++ +D++
Sbjct: 58 LASTDPDVPLEVRMDSQ 74
>YM53_MYCBO (P64956) Hypothetical protein Mb2253c
Length = 364
Score = 36.2 bits (82), Expect = 0.021
Identities = 24/77 (31%), Positives = 37/77 (47%), Gaps = 1/77 (1%)
Query: 27 IKINVDGSSFGNPDNAGFGGLL-RNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWD 85
+ I DG S GNP AG+G ++ + + + GRATN AE + GL A
Sbjct: 3 VVIEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVK 62
Query: 86 LGYRSIIMESDSQVALD 102
LG + DS++ ++
Sbjct: 63 LGATEAAVLMDSKLVVE 79
>YM28_MYCTU (P64955) Hypothetical protein Rv2228c/MT2287
Length = 364
Score = 36.2 bits (82), Expect = 0.021
Identities = 24/77 (31%), Positives = 37/77 (47%), Gaps = 1/77 (1%)
Query: 27 IKINVDGSSFGNPDNAGFGGLL-RNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWD 85
+ I DG S GNP AG+G ++ + + + GRATN AE + GL A
Sbjct: 3 VVIEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVK 62
Query: 86 LGYRSIIMESDSQVALD 102
LG + DS++ ++
Sbjct: 63 LGATEAAVLMDSKLVVE 79
>RNH_ECOLI (P00647) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
(Ribonuclease H) (RNase H)
Length = 155
Score = 33.9 bits (76), Expect = 0.11
Identities = 26/72 (36%), Positives = 36/72 (49%), Gaps = 4/72 (5%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDL 86
++I DGS GNP G+G +LR RG FS R TN EL A L+ +
Sbjct: 5 VEIFTDGSCLGNPGPGGYGAILR-YRGR-EKTFSAGYTRTTNNRMELMAAIVALEALKE- 61
Query: 87 GYRSIIMESDSQ 98
+ +I+ +DSQ
Sbjct: 62 -HCEVILSTDSQ 72
>RNH_THETH (P29253) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 166
Score = 32.7 bits (73), Expect = 0.23
Identities = 21/62 (33%), Positives = 29/62 (45%), Gaps = 2/62 (3%)
Query: 20 NPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKG 79
NP P + + DG+ GNP G+ LLR + + SG TN EL A +G
Sbjct: 2 NPSPRKRVALFTDGACLGNPGPGGWAALLRFHAHEKL--LSGGEACTTNNRMELKAAIEG 59
Query: 80 LQ 81
L+
Sbjct: 60 LK 61
>RNH_HAEDU (Q7VM15) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 153
Score = 32.0 bits (71), Expect = 0.40
Identities = 26/74 (35%), Positives = 37/74 (49%), Gaps = 8/74 (10%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCG--RATNLFAELSAIWKGLQLAW 84
+ I DGS GNP G G +LR N+ H S G + TN EL A+ +GL +
Sbjct: 4 VNIFTDGSCLGNPGPGGIGVVLRYNQ----HQKKVSQGYFQTTNNRMELRAVIEGLSMLK 59
Query: 85 DLGYRSIIMESDSQ 98
+ ++ + SDSQ
Sbjct: 60 EA--CNVTLYSDSQ 71
>RNH_XANCP (Q8PBX8) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 150
Score = 30.8 bits (68), Expect = 0.89
Identities = 23/72 (31%), Positives = 34/72 (46%), Gaps = 4/72 (5%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDL 86
I+++ DGS GNP G+ LLR N +G +TN EL A L+ +
Sbjct: 4 IEVHTDGSCLGNPGPGGWAALLRYNGRE--KELAGGEANSTNNRMELMAAIMALETLTE- 60
Query: 87 GYRSIIMESDSQ 98
I++ +DSQ
Sbjct: 61 -PCQILLHTDSQ 71
>RNH_XANAC (Q8PNH8) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 150
Score = 30.0 bits (66), Expect = 1.5
Identities = 23/72 (31%), Positives = 34/72 (46%), Gaps = 4/72 (5%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDL 86
I+++ DGS GNP G+ LLR N +G +TN EL A L+ +
Sbjct: 4 IEVHTDGSCLGNPGPGGWAALLRYNGRE--KELAGGEAVSTNNRMELMAAIMALETLTE- 60
Query: 87 GYRSIIMESDSQ 98
I++ +DSQ
Sbjct: 61 -PCQIVLHTDSQ 71
>RNH_ZYMMO (O69014) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 156
Score = 29.6 bits (65), Expect = 2.0
Identities = 20/57 (35%), Positives = 26/57 (45%), Gaps = 2/57 (3%)
Query: 24 EDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGL 80
+ + I DG+ GNP G+G LLR SGS TN EL A+ + L
Sbjct: 8 DKIVMIATDGACKGNPGFGGWGALLRYQGHE--KAISGSENPTTNNRMELQAVIEAL 62
>RNH_PHOLU (Q8GF77) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 156
Score = 29.6 bits (65), Expect = 2.0
Identities = 21/55 (38%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQ 81
++I DGS GNP G+G LLR + S R TN EL A GL+
Sbjct: 5 VEIFTDGSCLGNPGPGGYGVLLRYQQHE--KTLSEGFYRTTNNRMELMAAIIGLE 57
>RNH_SALTY (P23329) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
(Ribonuclease H) (RNase H)
Length = 155
Score = 29.3 bits (64), Expect = 2.6
Identities = 15/52 (28%), Positives = 26/52 (49%), Gaps = 2/52 (3%)
Query: 27 IKINVDGSSFGNPDNAGFGGLL--RNNRGNWIHGFSGSCGRATNLFAELSAI 76
++I DGS GNP G+G +L R + + G++ + L A + A+
Sbjct: 5 VEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSEGYTLTTNNRMELMAAIVAL 56
>RNH_HELPJ (Q9ZLH3) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 143
Score = 29.3 bits (64), Expect = 2.6
Identities = 20/56 (35%), Positives = 27/56 (47%), Gaps = 2/56 (3%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQL 82
I+I DGSS GNP G+ +LR SG TN EL A+ + L++
Sbjct: 4 IEIFCDGSSLGNPGPGGYAAILRYKDKE--KTISGGENFTTNNRMELRALNEALKI 57
>RNH_PASMU (P57813) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 154
Score = 28.9 bits (63), Expect = 3.4
Identities = 25/72 (34%), Positives = 32/72 (43%), Gaps = 4/72 (5%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDL 86
I+I DGS GNP G G LLR + S TN EL A+ + L +
Sbjct: 5 IEIFTDGSCLGNPGPGGIGVLLRYKQHE--KQISAGYFLTTNNRMELRAVIEALNTLKE- 61
Query: 87 GYRSIIMESDSQ 98
S+ + SDSQ
Sbjct: 62 -PCSVTLHSDSQ 72
>METH_MYCTU (O33259) Methionine synthase (EC 2.1.1.13)
(5-methyltetrahydrofolate--homocysteine
methyltransferase) (Methionine synthase, vitamin-B12
dependent isozyme) (MS)
Length = 1192
Score = 28.9 bits (63), Expect = 3.4
Identities = 13/36 (36%), Positives = 21/36 (58%)
Query: 92 IMESDSQVALDLILDTKQKDFHPHASLLSLIRKLSS 127
I E VALDL+ D +++D+ P L+ L +S+
Sbjct: 593 IPEEQRNVALDLVYDRRREDYDPLQELMRLFEGVSA 628
>Z276_HUMAN (Q8N554) Zinc finger protein 276 homolog (Zfp-276)
Length = 539
Score = 28.5 bits (62), Expect = 4.4
Identities = 12/31 (38%), Positives = 19/31 (60%), Gaps = 1/31 (3%)
Query: 55 WIHGFSGSCGRATNLFAELSAIWKG-LQLAW 84
W+HG + SCG +L LS+ + G +Q+ W
Sbjct: 127 WVHGHAASCGALPHLQRTLSSEYCGVIQVVW 157
>RNH_YERPE (Q8ZH30) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 154
Score = 28.5 bits (62), Expect = 4.4
Identities = 11/23 (47%), Positives = 15/23 (64%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLR 49
++I DGS GNP G+G +LR
Sbjct: 5 VEIFTDGSCLGNPGPGGYGAILR 27
>RNH_NEIMB (Q9JYE5) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 145
Score = 28.5 bits (62), Expect = 4.4
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQ 81
+ + DG+ GNP G+G L+R G+ G + TN EL+A+ +GL+
Sbjct: 5 VYLYTDGACKGNPGAGGWGVLMR--YGSHEKELFGGEAQTTNNRMELTAVIEGLK 57
>RNH_NEIMA (Q9JTD9) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 145
Score = 28.5 bits (62), Expect = 4.4
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQ 81
+ + DG+ GNP G+G L+R G+ G + TN EL+A+ +GL+
Sbjct: 5 VYLYTDGACKGNPGAGGWGVLMR--YGSHEKELFGGEAQTTNNRMELTAVIEGLK 57
>RNH_HELPY (P56120) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 143
Score = 28.5 bits (62), Expect = 4.4
Identities = 20/56 (35%), Positives = 27/56 (47%), Gaps = 2/56 (3%)
Query: 27 IKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQL 82
I+I DGSS GNP G+ +LR SG TN EL A+ + L++
Sbjct: 4 IEIFCDGSSLGNPGPGGYAAILRYKDKE--KTISGGEEFTTNNRMELRALNEALKI 57
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.140 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,676,310
Number of Sequences: 164201
Number of extensions: 693341
Number of successful extensions: 1441
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 1419
Number of HSP's gapped (non-prelim): 34
length of query: 136
length of database: 59,974,054
effective HSP length: 98
effective length of query: 38
effective length of database: 43,882,356
effective search space: 1667529528
effective search space used: 1667529528
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC121245.12