BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121242.1 + phase: 0 /pseudo
(403 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RNT1_DROME (Q9VYS3) Regulator of nonsense transcripts 1 homolog 37 0.090
WNK3_HUMAN (Q9BYP7) Serine/threonine-protein kinase WNK3 (EC 2.7... 34 0.76
SNE2_HUMAN (Q8WXH0) Nesprin 2 (Nuclear envelope spectrin repeat ... 32 2.2
GATB_SULSO (Q980Q3) Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransfe... 32 2.2
MYCD_RAT (Q8R5I7) Myocardin 32 2.9
HPPD_PIG (Q02110) 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.1... 32 3.8
YN53_CAEEL (P34587) Hypothetical protein ZC21.3 in chromosome III 31 4.9
NPBL_COPCI (Q00333) Rad9 protein (SCC2 homolog) 31 4.9
HSCA_RICPR (Q9ZDW5) Chaperone protein hscA homolog 31 4.9
GAG_BIV27 (P19559) Gag polyprotein (p53) [Contains: Matrix prote... 31 6.5
GAG_BIV06 (P19558) Gag polyprotein (p53) [Contains: Matrix prote... 31 6.5
CIW3_HUMAN (O14649) Potassium channel subfamily K member 3 (Acid... 31 6.5
COAT_CERV (P05399) Probable coat protein 30 8.4
>RNT1_DROME (Q9VYS3) Regulator of nonsense transcripts 1 homolog
Length = 1180
Score = 37.0 bits (84), Expect = 0.090
Identities = 31/134 (23%), Positives = 57/134 (42%), Gaps = 14/134 (10%)
Query: 239 VEALTQKIEKLNVKAVPPSSASPPCEVCGITGHIGVDCQLGSAANIEQLNYAQYNQGMRP 298
V+ LT+KI + N+K V VC + +D + A Q+ + N ++
Sbjct: 508 VDQLTEKIHRTNLKVV---------RVCAKSRE-AIDSPVSFLALHNQIRNMETNSELKK 557
Query: 299 NQNFYKNPQGSYGQAAPPGYTNNQRVAQKSSLE---ILLENCIANQNKNLQELKNQTGFL 355
Q K+ G A Y N +R A+ LE ++ C+ + L +K + +
Sbjct: 558 LQQL-KDETGELSSADEKRYRNLKRAAENQLLEAADVICCTCVGAGDGRLSRVKFTSILI 616
Query: 356 NDSLSPHTPKCLRP 369
++S+ P+C+ P
Sbjct: 617 DESMQSTEPECMVP 630
>WNK3_HUMAN (Q9BYP7) Serine/threonine-protein kinase WNK3 (EC
2.7.1.37) (Protein kinase with no lysine 3) (Protein
kinase, lysine-deficient 3)
Length = 1743
Score = 33.9 bits (76), Expect = 0.76
Identities = 22/71 (30%), Positives = 35/71 (48%), Gaps = 2/71 (2%)
Query: 322 QRVAQKSSLEILLENCIANQNKNLQELKNQTGFLNDSLSPHTPKCLRPKSPKWPNKWPPL 381
QR+ +K E++ N QNK LQEL + + DS + T L P SP+ P +
Sbjct: 1491 QRLREKHIQEVV--NLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSK 1548
Query: 382 LKHQESFLVNL 392
L+ + L ++
Sbjct: 1549 LRSRPQSLTHV 1559
>SNE2_HUMAN (Q8WXH0) Nesprin 2 (Nuclear envelope spectrin repeat
protein 2) (Syne-2) (Synaptic nuclear envelope protein 2)
(Nucleus and actin connecting element protein) (NUANCE
protein)
Length = 6885
Score = 32.3 bits (72), Expect = 2.2
Identities = 51/187 (27%), Positives = 76/187 (40%), Gaps = 22/187 (11%)
Query: 221 KKEAGIYE--VSEYNHLAAKVEALTQKIE---KLNVKAVPPSSASPPCEVCGITGHIGVD 275
K+E ++E SE++ A+++ L Q E +L V + + C +TG
Sbjct: 1683 KEELQVHEQKTSEFSRRVAEIQFLLQSSEIPLELQVMESSILNKMEHVQKC-LTGESNCH 1741
Query: 276 CQLGSAANI-EQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPPGYTNNQRVAQKSSLEILL 334
GS A + E L+ A+ GM + +P S Q + QK S+ ILL
Sbjct: 1742 ALSGSTAELREDLDQAKTQIGMTESLLKALSPSDSLEIFTKLEEIQQQILQQKHSM-ILL 1800
Query: 335 ENCIANQNKNLQELKNQTGFLND-------SLSPHTPKCLRPKSPKWPNKWPPLLKHQES 387
EN I L ELK Q ++D L H K L + + N W +K
Sbjct: 1801 ENQIGCLTPELSELKKQYESVSDLFNTKKSVLQDHFSKLLNDQCKNF-NDWFSNIK---- 1855
Query: 388 FLVNLKQ 394
VNLK+
Sbjct: 1856 --VNLKE 1860
>GATB_SULSO (Q980Q3) Aspartyl/glutamyl-tRNA(Asn/Gln)
amidotransferase subunit B (EC 6.3.5.-) (Asp/Glu-ADT
subunit B)
Length = 472
Score = 32.3 bits (72), Expect = 2.2
Identities = 46/196 (23%), Positives = 80/196 (40%), Gaps = 24/196 (12%)
Query: 48 GSPTEDPNLHISSFLRLS----GTIKENQEAVRLHLFPFSLRDRASAWFHSLEVGSITS- 102
G+ D N+ + R+ G+ K+ ++A+ + R RAS +++ T
Sbjct: 191 GAMKADVNISVKGGERVEVKNVGSPKDVEDAINYEI----ARQRASI-LQGIKIERETRH 245
Query: 103 WDDMRRAFLARFFPPSKTAKLRDQITRFNQKDGESLY---EAWERFKEMLRLCPHHGLEK 159
WD+ RR + P +T + + F D E E+FK + P E+
Sbjct: 246 WDNERRVTV-----PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAER 300
Query: 160 WLIVHTFYNGLSYTTKMSVDAAAGGALMNK--NYTEAYALIEDMAQNHY-QWTNERAVTT 216
++ YN Y ++ V+ A L + N + YA + ++ N Y +W NE +T
Sbjct: 301 FV---KQYNVTPYQAQVLVNEKALADLFEEITNNYKNYAKVANLLINDYMRWLNENNITV 357
Query: 217 PTPFKKEAGIYEVSEY 232
K I E+ EY
Sbjct: 358 TQSKAKANHIIELFEY 373
>MYCD_RAT (Q8R5I7) Myocardin
Length = 938
Score = 32.0 bits (71), Expect = 2.9
Identities = 35/148 (23%), Positives = 58/148 (38%), Gaps = 17/148 (11%)
Query: 273 GVDCQLGSAANIEQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPPGYTNNQRVAQKSSLEI 332
G + L AA+ G+ P+Q ++PQGS G T +++
Sbjct: 139 GTEVSLSKAADAFAFEDDSSRDGLSPDQARSEDPQGSGGSTPDIKSTEAPLAGPLDTIQD 198
Query: 333 LLENCIANQNKNLQELKNQT-------GFLNDSLSPHT---PKCL-----RPKSPKWPNK 377
L +++N +L NQ+ G L+ + HT K L R K PK P
Sbjct: 199 LTPGSESDKNDTASQLSNQSDSGKQVLGPLSTPIPVHTAVKSKSLGDSKNRHKKPKDPKP 258
Query: 378 WPPLLKHQESFLVNLK--QTPKPMSTLF 403
LK+ + + K ++P PM + +
Sbjct: 259 KVKKLKYHQYIPPDQKAEKSPPPMDSAY 286
>HPPD_PIG (Q02110) 4-hydroxyphenylpyruvate dioxygenase (EC
1.13.11.27) (4HPPD) (HPD) (HPPDase)
Length = 392
Score = 31.6 bits (70), Expect = 3.8
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 6/66 (9%)
Query: 195 YALIEDMAQNHYQWTNERAVTTPTPFKKEAGIYEVSEYN------HLAAKVEALTQKIEK 248
Y+ + + +Y+ + + + P P KK++ I E +YN H+A K E + I
Sbjct: 220 YSALRSVVMANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRS 279
Query: 249 LNVKAV 254
L + V
Sbjct: 280 LRERGV 285
>YN53_CAEEL (P34587) Hypothetical protein ZC21.3 in chromosome III
Length = 321
Score = 31.2 bits (69), Expect = 4.9
Identities = 25/90 (27%), Positives = 34/90 (37%), Gaps = 14/90 (15%)
Query: 272 IGVDCQLGSAANIEQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPPGYTNNQRVAQKSSLE 331
+GV+ ++N Q+ Q N NQ + N QG Y GY NNQ Q
Sbjct: 157 LGVNAGFPQSSNQNQVLRDQSN-----NQGYMNNNQGMYNTQTTQGYGNNQGSYQ----- 206
Query: 332 ILLENCIANQNKNLQELKNQTGFLNDSLSP 361
N + N N Q + T S+ P
Sbjct: 207 ----NQMMYNNNNPQNTQTTTFLYASSIQP 232
>NPBL_COPCI (Q00333) Rad9 protein (SCC2 homolog)
Length = 2157
Score = 31.2 bits (69), Expect = 4.9
Identities = 23/78 (29%), Positives = 34/78 (43%)
Query: 1 MAERTLKEYATPSTEEPQAIIVYPTVAGNNFEIKPALLNLVQQNQFSGSPTEDPNLHISS 60
+A +TL+E P P + V PT + N + K ALL+ V + + D +
Sbjct: 1343 LAMKTLEELWFPPLPPPSGMKVKPTSSSNPNQDKAALLSKVAIIMGTAANFRDRQSPLED 1402
Query: 61 FLRLSGTIKENQEAVRLH 78
L + KE EA LH
Sbjct: 1403 MLHKIISDKEGDEAASLH 1420
>HSCA_RICPR (Q9ZDW5) Chaperone protein hscA homolog
Length = 593
Score = 31.2 bits (69), Expect = 4.9
Identities = 24/82 (29%), Positives = 35/82 (42%), Gaps = 12/82 (14%)
Query: 111 LARF----FPPSKTAKLRDQITRFNQKDG---ESLYEAWERFKEMLRLCPHHGLEKWLIV 163
LARF PP K +R ++T DG S YE + + P+HG+ K I
Sbjct: 429 LARFELKGLPPMKAGNIRVEVTFAIDADGILSVSAYEKISNISHNIEIKPNHGINKTEIE 488
Query: 164 HTFYNG-----LSYTTKMSVDA 180
N + YTT++ +A
Sbjct: 489 TMLKNAYKNAKIDYTTRLLQEA 510
>GAG_BIV27 (P19559) Gag polyprotein (p53) [Contains: Matrix protein
p17; Capsid protein p26; Nucleocapsid protein p14]
Length = 476
Score = 30.8 bits (68), Expect = 6.5
Identities = 16/54 (29%), Positives = 26/54 (47%), Gaps = 4/54 (7%)
Query: 263 CEVCGITGHIGVDCQLGSAANIEQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPP 316
C CG GH +C+ + + + A Y Q +P NF+++ S +APP
Sbjct: 423 CYHCGKPGHQARNCRSKNG----KCSSAPYGQRSQPQNNFHQSNMSSVTPSAPP 472
>GAG_BIV06 (P19558) Gag polyprotein (p53) [Contains: Matrix protein
p17; Capsid protein p26; Nucleocapsid protein p14]
Length = 476
Score = 30.8 bits (68), Expect = 6.5
Identities = 16/54 (29%), Positives = 26/54 (47%), Gaps = 4/54 (7%)
Query: 263 CEVCGITGHIGVDCQLGSAANIEQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPP 316
C CG GH +C+ + + + A Y Q +P NF+++ S +APP
Sbjct: 423 CYHCGKPGHQARNCRSKNG----KCSSAPYGQRSQPQNNFHQSNMSSVTPSAPP 472
>CIW3_HUMAN (O14649) Potassium channel subfamily K member 3
(Acid-sensitive potassium channel protein TASK-1)
(TWIK-related acid-sensitive K+ channel 1) (Two pore
potassium channel KT3.1)
Length = 394
Score = 30.8 bits (68), Expect = 6.5
Identities = 23/77 (29%), Positives = 33/77 (41%), Gaps = 2/77 (2%)
Query: 122 KLRDQITRFNQKDGESLYEAWERFKEMLRLCPHHGLEKWLIVHTFYNGLSYTTKMSVDAA 181
+LR Q R + YE ER +LRL PH +W +FY ++ T + A
Sbjct: 42 ELRQQELRARYNLSQGGYEELERV--VLRLKPHKAGVQWRFAGSFYFAITVITTIGYGHA 99
Query: 182 AGGALMNKNYTEAYALI 198
A K + YAL+
Sbjct: 100 APSTDGGKVFCMFYALL 116
>COAT_CERV (P05399) Probable coat protein
Length = 494
Score = 30.4 bits (67), Expect = 8.4
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 2/64 (3%)
Query: 244 QKIEKLNVKAVPPSSASPPCEVCGITGHIGVDCQLGSAANIEQLNYAQYNQGMRPNQNFY 303
+K EK K P + C VC I GH +C + +L N G+ P +N Y
Sbjct: 403 KKSEK--AKYCPKGKKTCRCWVCNIEGHYANECPNRQTSEKFKLIQIAENYGLEPIENPY 460
Query: 304 KNPQ 307
++ Q
Sbjct: 461 EDQQ 464
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.131 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,874,863
Number of Sequences: 164201
Number of extensions: 2091744
Number of successful extensions: 5788
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 5780
Number of HSP's gapped (non-prelim): 17
length of query: 403
length of database: 59,974,054
effective HSP length: 112
effective length of query: 291
effective length of database: 41,583,542
effective search space: 12100810722
effective search space used: 12100810722
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 67 (30.4 bits)
Medicago: description of AC121242.1