BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121241.8 - phase: 0
(972 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
IKKE_MOUSE (Q9R0T8) Inhibitor of nuclear factor kappa-B kinase e... 37 0.26
SEC3_MOUSE (Q8R3S6) Exocyst complex component Sec3 37 0.33
IKKE_HUMAN (Q14164) Inhibitor of nuclear factor kappa-B kinase e... 36 0.44
YBY8_YEAST (P38277) Hypothetical 60.5 kDa protein in ESR1-IRA1 i... 35 0.97
DGT1_WOLSU (Q7MAE7) Deoxyguanosinetriphosphate triphosphohydrola... 35 0.97
YC72_CLOTE (Q895K1) Hypothetical UPF0090 protein CTC01272 35 1.3
MATK_FRAVE (Q8WJQ6) Maturase K (Intron maturase) 35 1.3
HMZ2_DROME (P09090) Zerknuellt protein 2 (ZEN-2) 33 2.8
SGT1_SCHPO (Q9US49) SGT1 protein homolog C1002.10c 33 4.8
UXAC_BACSU (O34808) Uronate isomerase (EC 5.3.1.12) (Glucuronate... 32 6.3
SYW_SALTY (Q8XGS1) Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Tr... 32 6.3
VI24_YEAST (P38263) Vacuolar import and degradation protein VID24 32 8.2
PARG_HUMAN (Q86W56) Poly(ADP-ribose) glycohydrolase (EC 3.2.1.143) 32 8.2
ILVD_HELHP (Q7VHW3) Dihydroxy-acid dehydratase (EC 4.2.1.9) (DAD) 32 8.2
DYHC_PARTE (Q27171) Dynein heavy chain, cytosolic (DYHC) 32 8.2
>IKKE_MOUSE (Q9R0T8) Inhibitor of nuclear factor kappa-B kinase
epsilon subunit (EC 2.7.1.-) (I kappa-B kinase epsilon)
(IkBKE) (IKK-epsilon) (IKK-E) (Inducible I kappa-B
kinase) (IKK-i)
Length = 717
Score = 37.0 bits (84), Expect = 0.26
Identities = 31/124 (25%), Positives = 53/124 (42%), Gaps = 7/124 (5%)
Query: 420 GGGSCVGILRPFTVSSALLSVLEDPQSASDFGAANMNSFIRTGI-----LKSDRIFHKNR 474
GG ++ + S +LLSVLEDP++ +R + L+ + I H R
Sbjct: 77 GGSRQKVLIMEYCSSGSLLSVLEDPENTFGLSEEEFLVVLRCVVAGMNHLRENGIVH--R 134
Query: 475 DLVDSQVKDVVGIKGEQKKKMTDLSALRNLTWSSFYDLVYDQFEMDLHEVYYAMECNKSK 534
D+ + +VG +G+ K++D A R L + VY E ++Y K +
Sbjct: 135 DIKPGNIMRLVGEEGQSIYKLSDFGAARKLDDDEKFVSVYGTEEYLHPDMYERAVLRKPQ 194
Query: 535 KLKF 538
+ F
Sbjct: 195 QKAF 198
>SEC3_MOUSE (Q8R3S6) Exocyst complex component Sec3
Length = 894
Score = 36.6 bits (83), Expect = 0.33
Identities = 40/175 (22%), Positives = 72/175 (40%), Gaps = 15/175 (8%)
Query: 500 ALRNLTWSSFYDLVYDQFEMDLH--EVYYAMECNKSKKLKFLKCWMK---QVKKSSCHDL 554
ALR+L D + + E DLH +VY + + ++K F+ C K + + +
Sbjct: 71 ALRDLAVVDAKDAIKENPEFDLHFEKVYKWVASSTAEKNAFISCIWKLNQRYLRKKIDFV 130
Query: 555 NLSEYPKPNQIFAEGTDSKLNELPQNGEQPIPQVVMSAGINAEVDTKKDDAVLDCGLETS 614
N+S +Q+ E S N+ G++ E D + +C + +
Sbjct: 131 NVS-----SQLLEESVPSGENQSVAGGDEEAVDEYQELNAREEQDIEIMMEGCECAISNA 185
Query: 615 EAFFRNLSNRIQQGIESDVIDLVALAERLVNSSIYWLCQKVDRETIPLIQVHSPL 669
EAF LS +Q ++ I + +E+ VN+ L Q +D + Q+ L
Sbjct: 186 EAFAEKLSRELQV-LDGANIQSIMASEKQVNT----LMQLLDEALTEVDQIELKL 235
>IKKE_HUMAN (Q14164) Inhibitor of nuclear factor kappa-B kinase
epsilon subunit (EC 2.7.1.-) (I kappa-B kinase epsilon)
(IkBKE) (IKK-epsilon) (IKK-E) (Inducible I kappa-B
kinase) (IKK-i)
Length = 716
Score = 36.2 bits (82), Expect = 0.44
Identities = 32/124 (25%), Positives = 53/124 (41%), Gaps = 7/124 (5%)
Query: 420 GGGSCVGILRPFTVSSALLSVLEDPQSASDFGAANMNSFIRTGI-----LKSDRIFHKNR 474
GG ++ + S +LLSVLE P++A +R + L+ + I H R
Sbjct: 77 GGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVH--R 134
Query: 475 DLVDSQVKDVVGIKGEQKKKMTDLSALRNLTWSSFYDLVYDQFEMDLHEVYYAMECNKSK 534
D+ + +VG +G+ K+TD A R L + VY E ++Y K +
Sbjct: 135 DIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQ 194
Query: 535 KLKF 538
+ F
Sbjct: 195 QKAF 198
>YBY8_YEAST (P38277) Hypothetical 60.5 kDa protein in ESR1-IRA1
intergenic region
Length = 524
Score = 35.0 bits (79), Expect = 0.97
Identities = 22/80 (27%), Positives = 43/80 (53%), Gaps = 3/80 (3%)
Query: 786 EDIVHKIYNKMDLLLFVNEDEAPDCSFNSEDSSKSLDRKFYGDEMGENDVGNGPFS-AEN 844
E +++I +++ + E C+ E+++K + + M E+++ +G FS EN
Sbjct: 359 EKALYEISSRLLNSGYYKEIVCEQCTVRYEEAAKIFENCEFESSMDESNLSSGTFSDLEN 418
Query: 845 --KPFHLQKNVRGKLQRNIE 862
+PFHL +V K+ R+IE
Sbjct: 419 SAEPFHLSTDVPKKINRHIE 438
>DGT1_WOLSU (Q7MAE7) Deoxyguanosinetriphosphate
triphosphohydrolase-like protein
Length = 365
Score = 35.0 bits (79), Expect = 0.97
Identities = 22/67 (32%), Positives = 32/67 (46%), Gaps = 10/67 (14%)
Query: 73 HLSFSKLHPFLPKHSFS--FDHPFSTFNLLSKTLSTFPNFPLTYNPKAANLIDSLTQ--L 128
H +L L KH F FDH F +F ++SK +PNF + NL + + L
Sbjct: 93 HAGGDELDRLLKKHGFEAGFDHNFQSFRVVSKLEKRYPNF------EGLNLTFATLEGIL 146
Query: 129 LHDYPWE 135
H YP++
Sbjct: 147 KHSYPYQ 153
>YC72_CLOTE (Q895K1) Hypothetical UPF0090 protein CTC01272
Length = 153
Score = 34.7 bits (78), Expect = 1.3
Identities = 28/95 (29%), Positives = 45/95 (46%), Gaps = 3/95 (3%)
Query: 764 FGDWNLENYVAKIIKSRYSHTLEDIVHKIYNKMDLLLFVNEDEAPDCSFNSEDSSKSLDR 823
F + N ENY+ I S LED K+ + +L +E + D S+ E SS L+R
Sbjct: 30 FVNENKENYLRIYIDSESGIGLEDC-EKVSRAVSAML--DEKDPIDTSYYLEVSSPGLER 86
Query: 824 KFYGDEMGENDVGNGPFSAENKPFHLQKNVRGKLQ 858
+ Y D+ E+++G F+ + GKL+
Sbjct: 87 QLYDDKHIEDNIGKTACVRLESLFNGGRKFEGKLK 121
>MATK_FRAVE (Q8WJQ6) Maturase K (Intron maturase)
Length = 500
Score = 34.7 bits (78), Expect = 1.3
Identities = 33/143 (23%), Positives = 59/143 (41%), Gaps = 20/143 (13%)
Query: 6 DPMIRY-SHTNRIVLLIDLDPLLLLLHNTTTTTNYIKNILSTSKTLFSFP------PLST 58
DP I+Y SH +++L PLL+ Y+ N ++S P LS
Sbjct: 266 DPFIQYVSHQGKLILASKDTPLLM-----NKWKYYLVNFWQCHFYVWSQPGRIHLNQLSK 320
Query: 59 SLFAFKFFFSSLPPHLSFSKLHPFLPKHSFSFDHPFSTFNLLSKTLSTFPNFPLTYNPKA 118
F F + SS+ P++S + S ++ + +N + K + FP P+ +
Sbjct: 321 HSFDFLGYLSSIRPNISVVR--------SQLLENTYLMYNAMKKLNTLFPIIPMIGSLAK 372
Query: 119 ANLIDSLTQLLHDYPWEPNSDAD 141
++L + W +SD+D
Sbjct: 373 VKFCNTLGHPISKSSWADSSDSD 395
>HMZ2_DROME (P09090) Zerknuellt protein 2 (ZEN-2)
Length = 252
Score = 33.5 bits (75), Expect = 2.8
Identities = 23/75 (30%), Positives = 36/75 (47%), Gaps = 10/75 (13%)
Query: 71 PPHLSFSKLHPFLPKHSFSFDHPFSTF--NLLSKTLSTFPNFPLTYNPKAANLIDSLTQ- 127
PP+ S+ LH F P+ PF+ F N S L P P+ A N+I+ TQ
Sbjct: 163 PPYQSYDYLHEFSPEPMALPQLPFNEFDANWASSWLGLEPTIPI-----AENVIEHNTQD 217
Query: 128 --LLHDYPWEPNSDA 140
++ ++ W+ NS +
Sbjct: 218 QPMIQNFCWDSNSSS 232
>SGT1_SCHPO (Q9US49) SGT1 protein homolog C1002.10c
Length = 590
Score = 32.7 bits (73), Expect = 4.8
Identities = 24/79 (30%), Positives = 40/79 (50%), Gaps = 4/79 (5%)
Query: 803 NEDEAP-DCSFNSEDSSKSLDRKFYGDEMGENDVGNGPFSAENKPFHLQKNVRGKLQRN- 860
NEDE N E+ +++D + YG G D G ++ +L KN+ ++ N
Sbjct: 510 NEDEMDIPGDANMEEYMRAMDEELYGGLRGR-DEGLEGIDDKDIDLNLMKNIIEGIEANP 568
Query: 861 -IEGGHNKTLIEALKIRMP 878
+ GG TL+ +LKI++P
Sbjct: 569 DLYGGPISTLLNSLKIQIP 587
>UXAC_BACSU (O34808) Uronate isomerase (EC 5.3.1.12) (Glucuronate
isomerase) (Uronic isomerase)
Length = 473
Score = 32.3 bits (72), Expect = 6.3
Identities = 40/163 (24%), Positives = 66/163 (39%), Gaps = 31/163 (19%)
Query: 271 SREVKVQLTLEILDVNGSPI---EYNCCDLEVLDFRVF-------GRGEDVNLQGGGRKE 320
+R++ V+ ++++ P+ EY+ E DF V +G ++N +G
Sbjct: 141 ARDLIVKSNVKVVCTTDDPVDSLEYHLLLKEDKDFPVSVLPGFRPDKGLEINREGFPE-- 198
Query: 321 RLWNVCSDGMAKLKVTVVRKCDAFVK--------FRSC---LSDSVLVREVLGECMKGDS 369
W + A + +T D F+K F S +SD + V E K ++
Sbjct: 199 --WVQALEDAAAISITTY---DEFLKALEKRVRFFHSAGGRVSDHAIDTMVFAETTKEEA 253
Query: 370 GGFFADRVLELLATEFGCQGRRKSVPVWEMLLSYLYKEDCWAL 412
G F+DR L TE C+ +K L LY E WA+
Sbjct: 254 GRIFSDR---LQGTEVSCEDEKKFKTYTLQFLCGLYAELDWAM 293
>SYW_SALTY (Q8XGS1) Tryptophanyl-tRNA synthetase (EC 6.1.1.2)
(Tryptophan--tRNA ligase) (TrpRS)
Length = 334
Score = 32.3 bits (72), Expect = 6.3
Identities = 34/139 (24%), Positives = 60/139 (42%), Gaps = 27/139 (19%)
Query: 687 LLKDPKDIAAKHKSRDSSSQAFDAAGPTIRYELQ----ILFRLEILQSEVGRGIEDSSKQ 742
LL+DPK + K K + S P +RY+++ + L+IL + G+ I + KQ
Sbjct: 209 LLEDPKSVVKKIKRAVTDSD----EPPVVRYDVKEKAGVSNLLDILSAVTGQSIPELEKQ 264
Query: 743 KFVKQICLLLENIQCHMEGGFFGDWNLENYVAKIIKSRYSHTLEDIVHKIYNKMDLLLFV 802
EG +G +L+ VA+ + S L++ H+ N L V
Sbjct: 265 ----------------FEGKMYG--HLKGEVAEAVSGMLSE-LQERYHRFRNDEAFLQKV 305
Query: 803 NEDEAPDCSFNSEDSSKSL 821
+D A S + ++ K++
Sbjct: 306 MKDGAEKASARAAETLKAV 324
>VI24_YEAST (P38263) Vacuolar import and degradation protein VID24
Length = 362
Score = 32.0 bits (71), Expect = 8.2
Identities = 18/87 (20%), Positives = 42/87 (47%), Gaps = 3/87 (3%)
Query: 468 RIFHKNRDLVDSQVKDVVGIKGEQKKKMTD---LSALRNLTWSSFYDLVYDQFEMDLHEV 524
RIF + + + + + +K ++K + D + + ++ FY +V+DQ ++
Sbjct: 271 RIFSQEKQFDNYLNERFIFMKWKEKFLVPDALLMEGVDGASYDGFYYIVHDQVTGNIQGF 330
Query: 525 YYAMECNKSKKLKFLKCWMKQVKKSSC 551
YY + K ++L+ + +V+ S C
Sbjct: 331 YYHQDAEKFQQLELVPSLKNKVESSDC 357
>PARG_HUMAN (Q86W56) Poly(ADP-ribose) glycohydrolase (EC 3.2.1.143)
Length = 976
Score = 32.0 bits (71), Expect = 8.2
Identities = 28/114 (24%), Positives = 47/114 (40%), Gaps = 18/114 (15%)
Query: 815 EDSSKSLDRKFYGDEMGENDVGNGP--FSAENKPFHLQKNVRGK-----LQRNIEGGHNK 867
E++S D + ++ + N P F A + +K K L EGG ++
Sbjct: 320 EETSPGFDEQ---EDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESR 376
Query: 868 TLIEALKIRMPA------LRVWAPKQKGMKSKK--DHLSKIPKRKDRTSACYDT 913
T + L ++P + KQ G K K DH ++PK +DR ++T
Sbjct: 377 TGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWET 430
>ILVD_HELHP (Q7VHW3) Dihydroxy-acid dehydratase (EC 4.2.1.9) (DAD)
Length = 572
Score = 32.0 bits (71), Expect = 8.2
Identities = 39/170 (22%), Positives = 72/170 (41%), Gaps = 12/170 (7%)
Query: 278 LTLEILDVNGSPIEYNCCDLEVLDFRVFGRGEDVNLQGGGRKERLWNVCSDGMAKLKVTV 337
L L+ L + G + + +++D V E+ GG K N+ +G A LKV
Sbjct: 350 LYLDALTITGETLGERIANAQIVDSSVIRHNENAYSPVGGLKILYGNLAREG-AVLKVAA 408
Query: 338 VRKCDAFVKFRSCLSDSVLVREVLGECMKGDSGGFFADRVLELLATEF--GCQGRRKSVP 395
V ++ +F S + E +KG +GG + ++ E G G ++ +
Sbjct: 409 V--AESMKEFEG----SAVCFNSQEEAIKGIAGGKVKAGNVVVIRYEGPKGGPGMQEMLT 462
Query: 396 VWEMLLSYLYKEDCWALVSVD--SGKGGGSCVGILRPFTVSSALLSVLED 443
+++ E AL++ SG G C+G + P L++++ED
Sbjct: 463 PTSLIMGMGLGESV-ALITDGRFSGATRGGCIGHISPEAAEGGLIALIED 511
>DYHC_PARTE (Q27171) Dynein heavy chain, cytosolic (DYHC)
Length = 4540
Score = 32.0 bits (71), Expect = 8.2
Identities = 34/127 (26%), Positives = 54/127 (41%), Gaps = 20/127 (15%)
Query: 572 SKLNELPQNGEQPIPQVVMSAGINAEVDTKKDDAV--LDCGLETSEAFFRNLSNRIQQGI 629
+K+ L Q GE+ I Q+ + + D KK D V L LE E F L N+I
Sbjct: 4242 TKVQNLQQEGEEEITQIEVQTEKTQKKDNKKSDQVQWLQDLLEKVEKFKAILPNKISP-- 4299
Query: 630 ESDVIDLVALAERLVNSSIYWLCQKVDRETIPLIQVHSPLKDN------NACGSMVVSEL 683
ER +S L + +DRE ++ ++ N A G ++ + +
Sbjct: 4300 ----------LERTADSINDPLFRFLDREITVASKLLKAVRQNIEELIQLAQGKILATNI 4349
Query: 684 VKQLLKD 690
++QL KD
Sbjct: 4350 LRQLAKD 4356
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.137 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 115,171,117
Number of Sequences: 164201
Number of extensions: 5038765
Number of successful extensions: 10892
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 10885
Number of HSP's gapped (non-prelim): 19
length of query: 972
length of database: 59,974,054
effective HSP length: 120
effective length of query: 852
effective length of database: 40,269,934
effective search space: 34309983768
effective search space used: 34309983768
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 71 (32.0 bits)
Medicago: description of AC121241.8