BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121237.2 - phase: 0
(463 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PGL2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.1... 113 1e-24
PGLS_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67)... 107 6e-23
PGLR_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67)... 105 2e-22
PGL2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.1... 104 5e-22
PGLR_AGRTU (P27644) Polygalacturonase (EC 3.2.1.15) (Pectinase) ... 101 5e-21
PGL2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (P... 99 2e-20
PGLT_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67)... 99 3e-20
PGLR_LYCES (P05117) Polygalacturonase 2A precursor (EC 3.2.1.15)... 98 5e-20
PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (P... 95 4e-19
PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (P... 88 5e-17
PGL1_ARATH (P49062) Exopolygalacturonase clone GBGE184 precursor... 84 8e-16
PGLR_PRUPE (P48979) Polygalacturonase precursor (EC 3.2.1.15) (P... 82 4e-15
PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (P... 81 5e-15
PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (P... 77 9e-14
PGLR_BRANA (P35337) Polygalacturonase precursor (EC 3.2.1.15) (P... 77 1e-13
PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (P... 75 3e-13
PGL1_ERWCA (P18192) Endo-polygalacturonase precursor (EC 3.2.1.15) 74 1e-12
PGLX_COCCA (Q00359) Exopolygalacturonase precursor (EC 3.2.1.67)... 73 1e-12
PGL2_ERWCA (P26509) Endo-polygalacturonase precursor (EC 3.2.1.15) 71 5e-12
PGLR_TOBAC (Q05967) Polygalacturonase precursor (EC 3.2.1.15) (P... 68 4e-11
>PGL2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.15)
(Pectinase) (Major pollen allergen Cha o 2)
Length = 514
Score = 113 bits (282), Expect = 1e-24
Identities = 107/384 (27%), Positives = 166/384 (42%), Gaps = 47/384 (12%)
Query: 45 FGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGS---FNLTSHFTLFLQK 101
+GAVGDGK +T+AF T + A L+VP K + F L +
Sbjct: 64 YGAVGDGKHDSTEAF---ATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLSFKV 120
Query: 102 DAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQGSY 161
D I+ D + W + + + LTD + G G IDGQG
Sbjct: 121 DGTIVAQPDPARWKNSKIWLQFAQ-----------------LTDFNLMG-TGVIDGQGQQ 162
Query: 162 WWDKFHKKQLTLT------RPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGL 215
WW K T RP I+I YS + + LTL+NSP + + + I GL
Sbjct: 163 WWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGL 222
Query: 216 TILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRR 275
I AP DSPNTDGI+ + IE I +GDDCIAI +G S I I+
Sbjct: 223 KIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------SSNITIKD 271
Query: 276 LTCISPDSAMV-ALGSEMS-GGIQDVRIEDVTAINTESAIRIKSAVGRGAFVKDIFVKGM 333
L C + +LG + S + V + I+T++ +RIK+ G I + +
Sbjct: 272 LICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENV 331
Query: 334 DLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAG-KLEGISNDPFT 392
++ + + Y + + +I G+ Y+++ + T A +L + P T
Sbjct: 332 EMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCT 391
Query: 393 GICVSNVTIEMSAHKKKLPWNCTD 416
GI +SNV++++++ K P +C D
Sbjct: 392 GIQLSNVSLKLTSGK---PASCVD 412
>PGLS_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 107 bits (267), Expect = 6e-23
Identities = 107/399 (26%), Positives = 176/399 (43%), Gaps = 60/399 (15%)
Query: 42 LTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHFT--LFL 99
+T GA G+GKT +TKA A S G +++P G +L G N T + +
Sbjct: 43 ITKLGASGNGKTDSTKAVQEAWA--SACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTI 100
Query: 100 QKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQG 159
Q D +L + D S++ + G + ++ NL +ITG G +DGQG
Sbjct: 101 QVDGNLLATTDLSQYKE--------------HGNWIEILRVDNL---VITG-KGNLDGQG 142
Query: 160 SYWWDKFH--KKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTI 217
W K KK P + + + + ++S +TL+NS + ++ N++I +T+
Sbjct: 143 PAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTV 202
Query: 218 LAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRRLT 277
AP DSPNTDGI+ + + I + I GDDCI+I G + ++ I +T
Sbjct: 203 TAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPG-----------TSKVNITGVT 251
Query: 278 CISPDSAMVALGS----EMSGGIQDVRIEDVTAINTESAIRIKSAVGRGAF--VKDIFVK 331
C P +++GS + + D+ ++D T T +RIK+ + V I +
Sbjct: 252 C-GPGHG-ISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYE 309
Query: 332 GMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISG---INYRDVTAKNVT-IAGKLEGIS 387
+ + ++ D + PN L +G + +DVT KN+T + E IS
Sbjct: 310 NIKMEDSANPIFI--------DMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAIS 361
Query: 388 -----NDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVT 421
P TG + +V +E S K CT+ G T
Sbjct: 362 LLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNAKGST 400
>PGLR_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 105 bits (262), Expect = 2e-22
Identities = 105/399 (26%), Positives = 176/399 (43%), Gaps = 60/399 (15%)
Query: 42 LTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHFT--LFL 99
+T GA G+GKT +TKA A S G +++P G +L G N T + +
Sbjct: 43 ITKLGASGNGKTDSTKAVQEAWA--SACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTI 100
Query: 100 QKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQG 159
Q D +L + D S++ G + ++ NL +ITG G +DGQG
Sbjct: 101 QVDGNLLATTDLSQYKD--------------HGNWIEILRVDNL---VITG-KGNLDGQG 142
Query: 160 SYWWDKFH--KKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTI 217
W K KK P + + + + ++S +TL+NS + ++ +++I +T+
Sbjct: 143 PAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTV 202
Query: 218 LAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRRLT 277
AP DSPNTDGI+ + + I + I GDDCI+I G + ++ I +T
Sbjct: 203 TAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPG-----------TSKVNITGVT 251
Query: 278 CISPDSAMVALGS----EMSGGIQDVRIEDVTAINTESAIRIKSAVGRGAF--VKDIFVK 331
C P +++GS + + D+ ++D T T +RIK+ + V I +
Sbjct: 252 C-GPGHG-ISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYE 309
Query: 332 GMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISG---INYRDVTAKNVT-IAGKLEGIS 387
+ + ++ D + PN L +G + +DVT KN+T + E +S
Sbjct: 310 NIKMEDSANPIFI--------DMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVS 361
Query: 388 -----NDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVT 421
P TG+ + +V +E S K CT+ G T
Sbjct: 362 LLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAKGST 400
>PGL2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.15)
(Pectinase) (Major pollen allergen Jun a 2)
Length = 507
Score = 104 bits (259), Expect = 5e-22
Identities = 114/419 (27%), Positives = 180/419 (42%), Gaps = 67/419 (15%)
Query: 45 FGAVGDGKTLNTKAF----NAAITNLSQYANDGGAQLIVPPGKWLTGSFNLT------SH 94
+GAVGDGK +T AF NAA LS A +VP K + NL H
Sbjct: 65 YGAVGDGKHDSTDAFEKTWNAACNKLS-------AVFLVPANKKFVVN-NLVFYGPCQPH 116
Query: 95 FTLFLQKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGT 154
F+ + D I D ++W + + R LTD + G G
Sbjct: 117 FSF--KVDGTIAAYPDPAKWKNSKIWMHFAR-----------------LTDFNLMG-TGV 156
Query: 155 IDGQGSYWWDKFHKKQLTLT------RPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSS 208
IDGQG+ WW K T RP I+I +S + + LTL NSP + +
Sbjct: 157 IDGQGNRWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECD 216
Query: 209 NIIINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPS 268
+ I G+ I AP DSPNTDGI+ + IE I +GDDC+A+ +G S
Sbjct: 217 GVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------S 265
Query: 269 QQIIIRRLTCISPDSAMVALGSEMSGG----IQDVRIEDVTAINTESAIRIKSAVGRGAF 324
I I+ LTC P M ++GS G + V ++ I+T++ +RIK+ G
Sbjct: 266 SNITIKDLTC-GPGHGM-SIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGL 323
Query: 325 VKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAG-KL 383
I + +++ + + Y + + KI + ++++ + T A +L
Sbjct: 324 ASHITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQL 383
Query: 384 EGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPCELLQ----EKEIE 438
+ P + I +SNV +++++ K N + +G +N + C+ L KE+E
Sbjct: 384 MCSDSVPCSNIKLSNVFLKLTSGKVATCVN-KNANGYYTNPLNPSCKSLHPGRTPKELE 441
>PGLR_AGRTU (P27644) Polygalacturonase (EC 3.2.1.15) (Pectinase)
(PGL)
Length = 312
Score = 101 bits (251), Expect = 5e-21
Identities = 69/225 (30%), Positives = 108/225 (47%), Gaps = 13/225 (5%)
Query: 179 IEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTILAPVDSPNTDGINPDSCTNVR 238
+ ++ + Q+ T+ N+ +W +HP ++ TI+AP DSPNTDG NP+SC NV
Sbjct: 25 LHLVSCHKTQLLGFTIRNAASWTIHPQGCEDLTAAASTIIAPHDSPNTDGFNPESCRNVM 84
Query: 239 IEDNFIVSGDDCIAIKSG-WDEYGIKVGMPSQQIIIRRLTCISPDSAMVALGSEMSGGIQ 297
I GDDCIA+K+G G + + I R + P + +GSEMSGG+
Sbjct: 85 ISGVRFSVGDDCIAVKAGKRGPDGEDDHLAETRGITVRHCLMQPGHGGLVIGSEMSGGVH 144
Query: 298 DVRIEDVTAINTESAIRIKS-AVGRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGF 356
DV +ED I T+ +R+K+ A G V +I ++ + L+ ++ Y D
Sbjct: 145 DVTVEDCDMIGTDRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYHCDADGHD 204
Query: 357 D----------PNALPKISGINYRDVTAKNVT-IAGKLEGISNDP 390
D + P + GI DV +N+ AG G+ + P
Sbjct: 205 DWVQSRNPAPVNDGTPFVDGITVEDVEIRNLAHAAGVFLGLPDVP 249
>PGL2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase) (Major pollen allergen Cry j 2) (Cry j II)
Length = 514
Score = 99.4 bits (246), Expect = 2e-20
Identities = 99/389 (25%), Positives = 170/389 (43%), Gaps = 52/389 (13%)
Query: 37 KHSAV----LTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGS---F 89
+H A+ + +GAVGDGK T+AF+ A A L+VP K + F
Sbjct: 52 RHDAINIFNVEKYGAVGDGKHDCTEAFSTA---WQAACKKPSAMLLVPGNKKFVVNNLFF 108
Query: 90 N--LTSHFTLFLQKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVI 147
N HFT + D +I Q+ + W + + + LT
Sbjct: 109 NGPCQPHFTF--KVDGIIAAYQNPASWKNNRIWLQFAK-----------------LTGFT 149
Query: 148 ITGDNGTIDGQGSYWWDKFHK----KQLT--LTRPYMIEIMYSDQIQISNLTLINSPTWF 201
+ G G IDGQG WW K +++ RP I+ +S + I L L+NSP +
Sbjct: 150 LMG-KGVIDGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFH 208
Query: 202 VHPIYSSNIIINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYG 261
+ + I G++I AP DSPNTDGI+ + N ++ N I +GDDC+AI +G
Sbjct: 209 LVFGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTG----- 263
Query: 262 IKVGMPSQQIIIRRLTCISPDSAMV-ALGSEMS-GGIQDVRIEDVTAINTESAIRIKSAV 319
S I+I L C + +LG E S + V + I+T++ +RIK+
Sbjct: 264 ------SSNIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQ 317
Query: 320 GRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTI 379
G I + +++ + + Y + + +I + Y+++ + T
Sbjct: 318 GGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATA 377
Query: 380 AG-KLEGISNDPFTGICVSNVTIEMSAHK 407
A +L+ + P I +S++++++++ K
Sbjct: 378 AAIQLKCSDSMPCKDIKLSDISLKLTSGK 406
>PGLT_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 98.6 bits (244), Expect = 3e-20
Identities = 102/397 (25%), Positives = 173/397 (42%), Gaps = 60/397 (15%)
Query: 42 LTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHFT--LFL 99
+T GA G+GKT +TKA A S G +++P G +L G N T + +
Sbjct: 43 ITKLGASGNGKTDSTKAVQEAWA--SACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTI 100
Query: 100 QKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQG 159
Q + +L + D S++ G + ++ NL +ITG G +DGQG
Sbjct: 101 QVNGNLLATTDLSQYKD--------------HGNWIEILRVDNL---VITG-KGKLDGQG 142
Query: 160 SYWWDKFH--KKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTI 217
W K KK P + + + + ++S +TL+NS + ++ +++I + +
Sbjct: 143 PAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNV 202
Query: 218 LAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRRLT 277
AP DSPNTDGI+ + V I + I GDDCI+I G + ++ I +T
Sbjct: 203 TAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPG-----------TSKVNITGVT 251
Query: 278 CISPDSAMVALGS----EMSGGIQDVRIEDVTAINTESAIRIKSAVGRGAFV--KDIFVK 331
C P +++GS + + D+ ++D T T + +RIK+ + + I +
Sbjct: 252 C-GPGHG-ISIGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYE 309
Query: 332 GMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISG---INYRDVTAKNVT------IAGK 382
+ + Y + D + PN L +G + +DVT KN+T A
Sbjct: 310 NIKMEDSGYPIII--------DMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVN 361
Query: 383 LEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISG 419
L + P TG+ + +V I+ S K C + G
Sbjct: 362 LLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKG 398
>PGLR_LYCES (P05117) Polygalacturonase 2A precursor (EC 3.2.1.15)
(PG-2A) (Pectinase)
Length = 457
Score = 97.8 bits (242), Expect = 5e-20
Identities = 100/421 (23%), Positives = 168/421 (39%), Gaps = 40/421 (9%)
Query: 26 NNFDYPAINCRKHSAVLTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWL 85
NN D N K VL+ FGA GDGKT + AF A Q +VP K
Sbjct: 63 NNIDKVDKNGIKVINVLS-FGAKGDGKTYDNIAFEQAWNEACSSRTP--VQFVVPKNK-- 117
Query: 86 TGSFNLTSHFTLFLQKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTD 145
+L K G S + + S + L +
Sbjct: 118 -----------NYLLKQITFSGPCRSSI--SVKIFGSLEASSKISDYKDRRLWIAFDSVQ 164
Query: 146 VIITGDNGTIDGQGSYWWD---KFHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFV 202
++ G GTI+G G WW K +K P + ++++NL N+ +
Sbjct: 165 NLVVGGGGTINGNGQVWWPSSCKINKSLPCRDAPTALTFWNCKNLKVNNLKSKNAQQIHI 224
Query: 203 HPIYSSNIIINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGI 262
+N++ + L I A SPNTDG++ + ++I D I +GDDCI+I SG
Sbjct: 225 KFESCTNVVASNLMINASAKSPNTDGVHVSNTQYIQISDTIIGTGDDCISIVSG------ 278
Query: 263 KVGMPSQQIIIRRLTCISPDSAMVALGSEMSGG----IQDVRIEDVTAINTESAIRIKSA 318
SQ + +TC P +++GS SG + +V + + I E+ +RIK+
Sbjct: 279 -----SQNVQATNITC-GPGHG-ISIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTW 331
Query: 319 VGRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVT 378
G +I +++ +KY + +Y D + + ++ + Y ++ + T
Sbjct: 332 QGGSGQASNIKFLNVEMQDVKYPIIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKGTSAT 391
Query: 379 -IAGKLEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPCELLQEKEI 437
+A K + +N P GI + N+ + + K C ++ + V C L+ E
Sbjct: 392 KVAIKFDCSTNFPCEGIIMENINL-VGESGKPSEATCKNVHFNNAEHVTPHCTSLEISED 450
Query: 438 E 438
E
Sbjct: 451 E 451
>PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 462
Score = 94.7 bits (234), Expect = 4e-19
Identities = 95/383 (24%), Positives = 173/383 (44%), Gaps = 42/383 (10%)
Query: 44 DFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGK-WLTG--SFNLTSHFTLFLQ 100
DFGA GDG T +TKAF A + A G+ LIVP K +L +F+ L ++
Sbjct: 89 DFGARGDG-TDDTKAFEKAWKD----ACSSGSVLIVPENKNYLLKQITFSGPCKSDLRVK 143
Query: 101 KDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQGS 160
I S D+S+W G R I +++++ + G GT +G G
Sbjct: 144 IRGTIEASSDQSDW--------VGHNR-------KRWIEFEDISNLTLEG-GGTSNGNGE 187
Query: 161 YWWDKFHKKQLTL---TRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTI 217
WWD K++ +L + P + + +S+L++ +S + ++I + L +
Sbjct: 188 TWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMV 247
Query: 218 LAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRRLT 277
AP SPNTDGI+ + + ++ I +GDDCI+I+SG S+ +I +T
Sbjct: 248 TAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESG-----------SKMVIATNIT 296
Query: 278 CISPDSAMV-ALGSEMS-GGIQDVRIEDVTAINTESAIRIKSAVGRGAFVKDIFVKGMDL 335
C + +LG S + V ++ +T + +RIK+ G K+I + + +
Sbjct: 297 CGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVM 356
Query: 336 NTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVT-IAGKLEGISNDPFTGI 394
+ + + Y D D + + K+S + Y ++ + + +A K + + P G
Sbjct: 357 HNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGY 416
Query: 395 CVSNVTIEMSAHKKKLPWNCTDI 417
V N+ + + K+ +C++I
Sbjct: 417 IVGNINL-VGNGGKETTMSCSNI 438
>PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 467
Score = 87.8 bits (216), Expect = 5e-17
Identities = 100/401 (24%), Positives = 174/401 (42%), Gaps = 52/401 (12%)
Query: 44 DFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTG--SFNLTSHFTLFLQK 101
DFGA GDG+ +TKAF A + ++ A L+VP +L SF+ L +Q
Sbjct: 94 DFGAKGDGRD-DTKAFEKAWK--AACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQI 150
Query: 102 DAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQGSY 161
I S D S++ + GR L+F + + ++ + G GTI+G G
Sbjct: 151 YGTIEASDDRSDYRK--------------DGRHW-LVFDS-VQNLRVEG-GGTINGNGKI 193
Query: 162 WWDKFHKKQLTLT---RPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTIL 218
WW K L P + S + + NL + N+ V N+ + L +
Sbjct: 194 WWQNSCKTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVT 253
Query: 219 APVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRRLTC 278
AP +SPNTDGI+ N+ I I +GDDCI+I +G S+++ + +TC
Sbjct: 254 APENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNG-----------SRKVRVNDITC 302
Query: 279 ISPDSAMVALGSEMSGG----IQDVRIEDVTAINTESAIRIKSAVGRGAFVKDIFVKGMD 334
P +++GS G + DV + T + +RIK+ G +I + ++
Sbjct: 303 -GPGHG-ISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVE 360
Query: 335 LNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDV---TAKNVTIAGKLEGISNDPF 391
++ ++ + +Y D + ++ ++ + Y+++ A NV I + P
Sbjct: 361 MHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAIT--FDCSKRFPC 418
Query: 392 TGICVSNVTIEM--SAHKKKLPWNCTDISGVTSNVVPKPCE 430
GI + +V +E+ A K L C ++ + VV C+
Sbjct: 419 QGIVLEDVDLEIEGGAAAKAL---CNNVELSETGVVSPHCQ 456
>PGL1_ARATH (P49062) Exopolygalacturonase clone GBGE184 precursor
(EC 3.2.1.67) (ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 422
Score = 84.0 bits (206), Expect = 8e-16
Identities = 84/342 (24%), Positives = 147/342 (42%), Gaps = 42/342 (12%)
Query: 42 LTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHFTLFLQK 101
+T FGAVGDG T KAF T + + A L+VP G +L G
Sbjct: 54 ITKFGAVGDGSTNTFKAF--LNTWIQVCDSPVPATLLVPKGTFLAGP------------- 98
Query: 102 DAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQGSY 161
VI +S+ + + G +G + F D ++ GT G+G
Sbjct: 99 --VIFAGPCKSK----VTVNVIGTIIATTSGYATPEWFLFERVDNLVLTGTGTFHGKGEA 152
Query: 162 WW--DKFHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLTILA 219
W D KK P ++ ++I+ ++ +N+ + + + + N+ I + + A
Sbjct: 153 VWKADGCGKKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTA 212
Query: 220 PVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRRLTCI 279
P +SPNTDGI+ + NV I D+ I +GDDC++ VG S + + R+ C
Sbjct: 213 PAESPNTDGIHLSNADNVSILDSTIATGDDCVS-----------VGRGSNNVTVERVICG 261
Query: 280 SPDSAMV-ALGS-EMSGGIQDVRIEDVTAINTESAIRIKSAVGRG-AFVKDIFVKGMDLN 336
V +LG + + + + + T I T++ +RIK+ G + DI + + +
Sbjct: 262 PGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQ 321
Query: 337 TMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVT 378
++K + +YG G D IS I ++++ +T
Sbjct: 322 SVKNPIIIDQNYG---SRGGDSQV--AISDILFKNIRGTTIT 358
>PGLR_PRUPE (P48979) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 393
Score = 81.6 bits (200), Expect = 4e-15
Identities = 110/404 (27%), Positives = 175/404 (43%), Gaps = 57/404 (14%)
Query: 42 LTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHFTLFLQK 101
+ GA DGKT +TKAF +A N G + VP G T FL +
Sbjct: 30 VASLGAKADGKTDSTKAFLSAWAKACASMNPG--VIYVPAG-------------TFFL-R 73
Query: 102 DAVILGSQDES----EWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDG 157
D V G + LV PS R G ++ IF ++ V I+G G +DG
Sbjct: 74 DVVFSGPCKNNAITFRIAGTLVAPSDYR----VIGNAANWIFFHHVNGVTISG--GILDG 127
Query: 158 QGSYWWD-KFHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIIINGLT 216
QG+ W K + + + S+ I +S L +NS + + N+ + G+
Sbjct: 128 QGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVR 187
Query: 217 ILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSG----WDEYGIKVGMPSQQII 272
+ +SPNTDGI+ + V I ++ I +GDDC++I G W E G+ G P I
Sbjct: 188 VSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIE-GVACG-PGHGIS 245
Query: 273 IRRLTCISPDSAMVALGSEM-SGGIQDVRIEDVTAINTESAIRIKSAVGRGA--FVKDIF 329
I +LG E G+Q+V ++ VT T++ +RIKS GR + F ++I
Sbjct: 246 IG------------SLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKS-WGRPSTGFARNIL 292
Query: 330 VKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALP--KISGINYRDVTAKNVT-IAGKLEGI 386
+ + ++ + Y PDN P + +IS + Y D+ + T +A K +
Sbjct: 293 FQHATMVNVENPIVIDQHY--CPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCS 350
Query: 387 SNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVV-PKPC 429
P I + +V +++ + +C+ G T VV P C
Sbjct: 351 PKHPCREIKLEDV--KLTYKNQAAESSCSHADGTTEGVVQPTSC 392
>PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 460
Score = 81.3 bits (199), Expect = 5e-15
Identities = 82/320 (25%), Positives = 140/320 (43%), Gaps = 48/320 (15%)
Query: 44 DFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTG--SFNLTSHFTLFLQK 101
DFGA G+G +T+AF A + ++ G L+VP +L F+ L LQ
Sbjct: 103 DFGAKGNGAD-DTQAFVKAWK--AACSSSGAMVLVVPQKNYLVRPIEFSGPCKSQLTLQI 159
Query: 102 DAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSLIFGTNLTDVIITGDNGTIDGQGSY 161
I S+D S + + + N+ ++++ G GTI+G G+
Sbjct: 160 YGTIEASEDRSIYKDI-----------------DHWLIFDNVQNLLVVGP-GTINGNGNI 201
Query: 162 WWDKFHKKQ----LTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSS--NIIINGL 215
WW K + P + + + + NL + ++ +H I+ + N+ + L
Sbjct: 202 WWKNSCKIKPQPPCGTYAPTAVTFNRCNNLVVKNLNIQDAQQ--IHVIFQNCINVQASCL 259
Query: 216 TILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQIIIRR 275
T+ AP DSPNTDGI+ + N+ I + I +GDDCI+I SG SQ++
Sbjct: 260 TVTAPEDSPNTDGIHVTNTQNITISSSVIGTGDDCISIVSG-----------SQRVQATD 308
Query: 276 LTCISPDSAMVALGSEMSGGIQD----VRIEDVTAINTESAIRIKSAVGRGAFVKDIFVK 331
+TC P +++GS G +D V + T + +RIK+ G +I +
Sbjct: 309 ITC-GPGHG-ISIGSLGEDGSEDHVSGVFVNGAKLSGTSNGLRIKTWKGGSGSATNIVFQ 366
Query: 332 GMDLNTMKYVFWMTGSYGDH 351
+ +N + + +Y DH
Sbjct: 367 NVQMNDVTNPIIIDQNYCDH 386
>PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 407
Score = 77.0 bits (188), Expect = 9e-14
Identities = 93/404 (23%), Positives = 170/404 (42%), Gaps = 54/404 (13%)
Query: 41 VLTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTS--HFTLF 98
V+ FGA DGKT +K F A A+ + +++P G +L NL +
Sbjct: 31 VVAKFGAKADGKTDLSKPFLDAWKEAC--ASVTPSTVVIPKGTYLLSKVNLEGPCKAPIE 88
Query: 99 LQKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSL----IFGTNLTDVIITGDNGT 154
+ I D S + +D RF S+ +FG G
Sbjct: 89 INVQGTIQAPADPSAF------------KDPNWVRFYSVENFKMFG-----------GGI 125
Query: 155 IDGQGSYWWDK--FHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIII 212
DGQGS ++K ++ P I + I ++T +S + ++ NI +
Sbjct: 126 FDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITL 185
Query: 213 NGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQII 272
L I AP +SPNTDGI+ V I + I +GDDCI+I G ++ ++
Sbjct: 186 ERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDG-----------TKNMV 234
Query: 273 IRRLTCISPDSAMVALGS----EMSGGIQDVRIEDVTAINTESAIRIKSAVG-RGAFVKD 327
I+ +TC P +++GS + ++ ++I + T NT + RIK+ G G V +
Sbjct: 235 IKEITC-GPGHG-ISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSE 292
Query: 328 IFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKN-VTIAGKLEGI 386
I + + +N + + Y + + K+S I+++++ + + A K
Sbjct: 293 IHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICS 352
Query: 387 SNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVV-PKPC 429
+ P + ++++ I+ + + C ++ +TS + P PC
Sbjct: 353 GSSPCQNVELADIDIKHNGAEPATS-QCLNVKPITSGKLNPIPC 395
>PGLR_BRANA (P35337) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 397
Score = 76.6 bits (187), Expect = 1e-13
Identities = 67/284 (23%), Positives = 129/284 (44%), Gaps = 40/284 (14%)
Query: 153 GTIDGQGSYWW--DKFHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNI 210
GT DG+G+ W + HK P + + D +I ++T +++ + + I N+
Sbjct: 113 GTFDGEGNAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKNFHFNVISGKNM 172
Query: 211 IINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQ 270
+ + I+AP +SPNTDGI+ C V+I + I +GDDCI++ G +
Sbjct: 173 TFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDG-----------MKN 221
Query: 271 IIIRRLTCISPDSAMVALGSEMSGG----IQDVRIEDVTAINTESAIRIK-------SAV 319
++I ++ C P +++GS G + D+ +++ T T + +RIK +
Sbjct: 222 LLIEKVVC-GPGHG-ISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTT 279
Query: 320 GRGAFVKDIFVKGMD---LNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDV--TA 374
G +DI + + L +Y W + N P+ + K+ I +R++ T+
Sbjct: 280 AAGIHFEDIILNKVSNPILIDQEYCPW-------NQCNKNKPSTI-KLVDITFRNIRGTS 331
Query: 375 KNVTIAGKLEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDIS 418
+N A KL P + + ++ IE + + CT+++
Sbjct: 332 ENKD-AVKLLCSKGHPCENVEIGDINIEYTGPDGPPTFECTNVT 374
>PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 407
Score = 75.5 bits (184), Expect = 3e-13
Identities = 92/404 (22%), Positives = 169/404 (41%), Gaps = 54/404 (13%)
Query: 41 VLTDFGAVGDGKTLNTKAFNAAITNLSQYANDGGAQLIVPPGKWLTGSFNLTS--HFTLF 98
V+ FGA DGKT +K F A A+ + +++P G +L NL +
Sbjct: 31 VVAKFGAKADGKTDLSKPFLDAWKEAC--ASVTPSTVVIPKGTYLLSKVNLEGPCKAPIE 88
Query: 99 LQKDAVILGSQDESEWPQLLVLPSYGRGRDAPAGRFSSL----IFGTNLTDVIITGDNGT 154
+ I D S + +D RF S+ +FG G
Sbjct: 89 INVQGTIQAPADPSAF------------KDPNWVRFYSVENFKMFG-----------GGI 125
Query: 155 IDGQGSYWWDK--FHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHPIYSSNIII 212
DGQGS ++K ++ P I + I ++T +S + ++ NI +
Sbjct: 126 FDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITL 185
Query: 213 NGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYGIKVGMPSQQII 272
L I AP +SPNTDGI+ V I + I +GDDCI+I G ++ ++
Sbjct: 186 ERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDG-----------TKNMV 234
Query: 273 IRRLTCISPDSAMVALGS----EMSGGIQDVRIEDVTAINTESAIRIKSAVG-RGAFVKD 327
I+ +TC P +++GS + ++ ++I + T NT + RIK+ G G V +
Sbjct: 235 IKEITC-GPGHG-ISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSE 292
Query: 328 IFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKN-VTIAGKLEGI 386
I + + +N + + Y + + K+S I+++++ + + A K
Sbjct: 293 IHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICS 352
Query: 387 SNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVT-SNVVPKPC 429
+ P + ++++ I+ + + C ++ +T + P PC
Sbjct: 353 GSSPCQNVELADIDIQHNGAEPATS-QCLNVKPITIGKLNPIPC 395
>PGL1_ERWCA (P18192) Endo-polygalacturonase precursor (EC 3.2.1.15)
Length = 402
Score = 73.6 bits (179), Expect = 1e-12
Identities = 80/307 (26%), Positives = 133/307 (43%), Gaps = 37/307 (12%)
Query: 84 WLTGSFNLTSHFTLFLQKDAVILGSQDESEWPQLL----VLPSYGRGRDAPAGRFSSLIF 139
+L+G +L S +L + K + + + V+ + G+G DA I
Sbjct: 78 FLSGPLSLPSGVSLLIDKGVTLRAVNNAKSFENAPSSCGVVDTNGKGCDA-------FIT 130
Query: 140 GTNLTDVIITGDNGTIDGQGSY--------WWD---KFHKKQLTLTRPYMIEIMYSDQIQ 188
T+ T+ I G GTIDGQG WWD K+L P +I+I S
Sbjct: 131 ATSTTNSGIYGP-GTIDGQGGVKLQDKKVSWWDLAADAKVKKLKQNTPRLIQINKSKNFT 189
Query: 189 ISNLTLINSPTWFVHPIYSSNIIINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGD 248
+ N++LINSP + V TI P + NTDGI+P S N+ I + I +GD
Sbjct: 190 LYNVSLINSPNFHVVFSDGDGFTAWKTTIKTPSTARNTDGIDPMSSKNITIAHSNISTGD 249
Query: 249 DCIAIKSGWDEYGIKVGMPSQQIIIRRLTCISPDSAMVALGSEMSGGIQDVRIEDVTAIN 308
D +AIK+ K ++ I I L +++GSE + G+ +V ++D+
Sbjct: 250 DNVAIKA------YKGRSETRNISI--LHNEFGTGHGMSIGSE-TMGVYNVTVDDLIMTG 300
Query: 309 TESAIRIKSAVGRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGIN 368
T + +RIKS V + + + + + Y + + +P S I
Sbjct: 301 TTNGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVIDTVYEKK-----EGSNVPDWSDIT 355
Query: 369 YRDVTAK 375
++D+T++
Sbjct: 356 FKDITSQ 362
>PGLX_COCCA (Q00359) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Galacturan 1,4-alpha-galacturonidase)
(Poly(1,4-alpha-D-galacturonide)galacturonohydrolase)
Length = 446
Score = 73.2 bits (178), Expect = 1e-12
Identities = 71/307 (23%), Positives = 129/307 (41%), Gaps = 44/307 (14%)
Query: 145 DVIITGDNGTIDGQGSYWWDKFHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWFVHP 204
D+ + G GT+DG G W+D + K + + RP + ++ + +IS+L SP W+
Sbjct: 145 DINVYG-GGTLDGNGQAWYD-LYAKDIYILRPILFGLIGAKNAKISDLKFRYSPQWYTLV 202
Query: 205 IYSSNIIINGLTILAPVDS----PNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEY 260
SS ++ + + I S NTDG + N+ I+++ I +GDDC++ K
Sbjct: 203 ANSSQVVFSNIDIFGDSKSKNPAKNTDGWDTYRSDNIIIQNSNINNGDDCVSFKPN---- 258
Query: 261 GIKVGMPSQQIIIRRLTCISPDSAMVALGSEMSGG---IQDVRIEDVTAINTESAIRIK- 316
S I+++ L C V + G ++++ + +++ N RIK
Sbjct: 259 -------STNILVQNLVCNGSHGISVGSLGQYPGEVDIVENILVRNISMSNASDGARIKV 311
Query: 317 -----SAV-------GRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKI 364
SA+ G V+++ GM + + Y +T YG N N P
Sbjct: 312 WPGASSALSGDLQGGGGSGAVRNVTYDGMIVKNVDYAIEITQCYGQ--KNLTLCNQFP-- 367
Query: 365 SGINYRDVTAKNV--TIAGKLEG-----ISNDPFTGICVSNVTIEMSAHKKKLPWNCTDI 417
S + D+T KN T + K + + + P +S I++ + + C +
Sbjct: 368 SNLTISDITIKNFKGTTSKKYDPRVGYVVCSSPKVCSDISIENIDVKSPSGTNLFTCANA 427
Query: 418 SGVTSNV 424
G+ S V
Sbjct: 428 EGIQSQV 434
>PGL2_ERWCA (P26509) Endo-polygalacturonase precursor (EC 3.2.1.15)
Length = 402
Score = 71.2 bits (173), Expect = 5e-12
Identities = 91/365 (24%), Positives = 154/365 (41%), Gaps = 53/365 (14%)
Query: 39 SAVLTDFGAVGDGKTLNT----KAFNAAITNLSQYAN---DGGAQLIVPPGK---WLTGS 88
SA +D V + KT ++ KA ++ T+ Q A D G + + G +L+G
Sbjct: 23 SAWASDSRTVSEPKTPSSCTTLKADSSTATSTIQKALNNCDQGKAVRLSAGSTSVFLSGP 82
Query: 89 FNLTSHFTLFLQKDAVILGSQDESEWPQLL----VLPSYGRGRDAPAGRFSSLIFGTNLT 144
+L S +L + K + + + V+ G+G DA I + T
Sbjct: 83 LSLPSGVSLLIDKGVTLRAVNNAKSFENAPSSCGVVDKNGKGCDA-------FITAVSTT 135
Query: 145 DVIITGDNGTIDGQGSY--------WWD---KFHKKQLTLTRPYMIEIMYSDQIQISNLT 193
+ I G GTIDGQG WW+ K+L P +I+I S + N++
Sbjct: 136 NSGIYGP-GTIDGQGGVKLQDKKVSWWELAADAKVKKLKQNTPRLIQINKSKNFTLYNVS 194
Query: 194 LINSPTWFVHPIYSSNIIINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAI 253
LINSP + V TI P + NTDGI+P S N+ I + I +GDD +AI
Sbjct: 195 LINSPNFHVVFSDGDGFTAWKTTIKTPSTARNTDGIDPMSSKNITIAYSNIATGDDNVAI 254
Query: 254 KSGWDEYGIKVGMPSQQIIIRRLTCISPDSAM---VALGSEMSGGIQDVRIEDVTAINTE 310
K+ + R ++ + D +++GSE + G+ +V ++D+ T
Sbjct: 255 KA-----------YKGRAETRNISILHNDFGTGHGMSIGSE-TMGVYNVTVDDLKMNGTT 302
Query: 311 SAIRIKSAVGRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYR 370
+ +RIKS V + + + + + Y + + +P S I ++
Sbjct: 303 NGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVIDTVYEKK-----EGSNVPDWSDITFK 357
Query: 371 DVTAK 375
DVT++
Sbjct: 358 DVTSE 362
>PGLR_TOBAC (Q05967) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 396
Score = 68.2 bits (165), Expect = 4e-11
Identities = 65/280 (23%), Positives = 117/280 (41%), Gaps = 16/280 (5%)
Query: 142 NLTDVIITGDNGTIDGQGSYWWDKFHKKQLTLTRPYMIEIMYSDQIQISNLTLINSPTWF 201
N D G +DGQ + W+ K+ P + I ++T ++S ++
Sbjct: 108 NKLDQFTMSGGGILDGQAAAAWECKQSKKCNKL-PNNLSFNSLTNSTIKDITTLDSKSFH 166
Query: 202 VHPIYSSNIIINGLTILAPVDSPNTDGINPDSCTNVRIEDNFIVSGDDCIAIKSGWDEYG 261
V+ N+ + AP +SPNTDGI+ ++V I D+ +GDDCI+
Sbjct: 167 VNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCIS--------- 217
Query: 262 IKVGMPSQQIIIRRLTCISPDSAMVAL--GSEMSGGIQDVRIEDVTAINTESAIRIKS-A 318
VG ++Q+ I R+TC V G+ + V + + T NT++ +RIK+
Sbjct: 218 --VGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWP 275
Query: 319 VGRGAFVKDIFVKGMDLNTMKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVT 378
V D+ + + + + + Y D + KIS ++++++ + T
Sbjct: 276 ASHPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRT 335
Query: 379 -IAGKLEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDI 417
A L P GI V ++ I S + +C +I
Sbjct: 336 QDAVSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENI 375
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.137 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,786,569
Number of Sequences: 164201
Number of extensions: 2546977
Number of successful extensions: 5619
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 35
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 5493
Number of HSP's gapped (non-prelim): 84
length of query: 463
length of database: 59,974,054
effective HSP length: 114
effective length of query: 349
effective length of database: 41,255,140
effective search space: 14398043860
effective search space used: 14398043860
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC121237.2