BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147429.11 - phase: 0
(149 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM64843.1| unknown [Arabidopsis thaliana] gi|20198305|gb|AAB... 130 5e-30
pir||A84823 hypothetical protein At2g39920 [imported] - Arabidop... 130 5e-30
emb|CAB79685.1| acid phosphatase-like protein [Arabidopsis thali... 49 2e-05
emb|CAB79684.1| acid phosphatase-like protein [Arabidopsis thali... 41 0.005
gb|AAC67358.1| putative acid phosphatase [Arabidopsis thaliana] ... 40 0.012
emb|CAA39370.1| acid phosphatase [Lycopersicon esculentum] gi|74... 40 0.015
prf||1908418A acid phosphatase 1 40 0.015
ref|XP_479551.1| putative syringolide-induced protein [Oryza sat... 38 0.044
dbj|BAD95053.1| acid phosphatase [Arabidopsis thaliana] gi|88438... 38 0.044
dbj|BAB86895.1| syringolide-induced protein B15-3-5 [Glycine max] 37 0.075
ref|NP_765042.1| replication-associated protein [Staphylococcus ... 36 0.22
ref|ZP_00323306.1| COG0657: Esterase/lipase [Pediococcus pentosa... 35 0.28
gb|AAC60539.2| acid phosphatase-1(1); Apase-1(1) [Lycopersicon e... 35 0.37
ref|NP_376216.1| hypothetical spermidine synthase [Sulfolobus to... 34 0.83
gb|EAA20715.1| hypothetical protein [Plasmodium yoelii yoelii] 34 0.83
ref|NP_473276.1| hypothetical protein [Plasmodium falciparum 3D7... 33 1.1
emb|CAD62799.1| amino acid transport protein [Lactobacillus plan... 33 1.1
ref|YP_176402.1| D-alanine--D-alanine ligase A [Bacillus clausii... 33 1.4
dbj|BAC82457.1| pod storage protein [Phaseolus vulgaris] gi|7442... 33 1.4
gb|AAL17638.1| putative defense associated acid phosphatase [Pha... 33 1.4
>gb|AAM64843.1| unknown [Arabidopsis thaliana] gi|20198305|gb|AAB95277.2| expressed
protein [Arabidopsis thaliana]
gi|18405204|ref|NP_565918.1| acid phosphatase class B
family protein [Arabidopsis thaliana]
Length = 283
Score = 130 bits (328), Expect = 5e-30
Identities = 63/134 (47%), Positives = 94/134 (70%), Gaps = 1/134 (0%)
Query: 1 MGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNGGIIELRNIN 60
+GS + +ESG Y+TS +A+IFIA G+L++TLL++++ MLQSC+N N I+E + ++
Sbjct: 23 LGSRYSIESGCYMTSLAASIFIASLVTFGVLMITLLIALSTMLQSCENRNIAIVEAQRLD 82
Query: 61 NDYSYCKIHSLHAKLNNL-EEHNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVK 119
+ YCKI SLH++LN+L EE +P +C+D+AL IK G Y R+L+ T + YF +K
Sbjct: 83 ESFGYCKILSLHSQLNSLDEESELPLLCRDVALHRIKQGIYLRELNFTIQMALTYFQTIK 142
Query: 120 PSEDGFDVVLIDID 133
P D DVV+IDID
Sbjct: 143 PMNDNCDVVVIDID 156
>pir||A84823 hypothetical protein At2g39920 [imported] - Arabidopsis thaliana
Length = 236
Score = 130 bits (328), Expect = 5e-30
Identities = 63/134 (47%), Positives = 94/134 (70%), Gaps = 1/134 (0%)
Query: 1 MGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNGGIIELRNIN 60
+GS + +ESG Y+TS +A+IFIA G+L++TLL++++ MLQSC+N N I+E + ++
Sbjct: 23 LGSRYSIESGCYMTSLAASIFIASLVTFGVLMITLLIALSTMLQSCENRNIAIVEAQRLD 82
Query: 61 NDYSYCKIHSLHAKLNNL-EEHNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVK 119
+ YCKI SLH++LN+L EE +P +C+D+AL IK G Y R+L+ T + YF +K
Sbjct: 83 ESFGYCKILSLHSQLNSLDEESELPLLCRDVALHRIKQGIYLRELNFTIQMALTYFQTIK 142
Query: 120 PSEDGFDVVLIDID 133
P D DVV+IDID
Sbjct: 143 PMNDNCDVVVIDID 156
>emb|CAB79685.1| acid phosphatase-like protein [Arabidopsis thaliana]
gi|21536678|gb|AAM61010.1| acid phosphatase-like protein
[Arabidopsis thaliana] gi|28466939|gb|AAO44078.1|
At4g29270 [Arabidopsis thaliana]
gi|15233526|ref|NP_194656.1| acid phosphatase class B
family protein [Arabidopsis thaliana]
gi|7435100|pir||T13440 acid phosphatase homolog
T17A13.90 - Arabidopsis thaliana
Length = 256
Score = 48.9 bits (115), Expect = 2e-05
Identities = 28/71 (39%), Positives = 37/71 (51%), Gaps = 1/71 (1%)
Query: 64 SYCKIHSLHAKLNNLEEHNV-PNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSE 122
SYC+ L A+ NN+ V P+ C++ YI GGQ+ +D D S DY VK
Sbjct: 42 SYCESWRLAAETNNVGPWKVIPSQCENYIKNYINGGQFDKDYDVVASYAIDYAKTVKVGG 101
Query: 123 DGFDVVLIDID 133
DG D + DID
Sbjct: 102 DGKDAWVFDID 112
>emb|CAB79684.1| acid phosphatase-like protein [Arabidopsis thaliana]
gi|20258885|gb|AAM14114.1| putative acid phosphatase
[Arabidopsis thaliana] gi|15293023|gb|AAK93622.1|
putative acid phosphatase [Arabidopsis thaliana]
gi|15233504|ref|NP_194655.1| acid phosphatase class B
family protein [Arabidopsis thaliana]
gi|7435101|pir||T13437 acid phosphatase homolog
T17A13.80 - Arabidopsis thaliana
Length = 255
Score = 41.2 bits (95), Expect = 0.005
Identities = 29/80 (36%), Positives = 39/80 (48%), Gaps = 9/80 (11%)
Query: 65 YCKIHSLHAKLNNLEEHN-VPNICKDLALQYIKGGQYARDLDSTKSVIEDYF----NGVK 119
YC L A+ NN+ + +P+IC D +Y+ G Q+ D SVI DY V+
Sbjct: 42 YCDSWRLAAETNNVGTWDLIPSICVDSVAEYLNGDQFLSDY----SVIVDYALAFAKSVE 97
Query: 120 PSEDGFDVVLIDIDSLFQWN 139
S DG DV + DID N
Sbjct: 98 ISGDGKDVWIFDIDETLLTN 117
>gb|AAC67358.1| putative acid phosphatase [Arabidopsis thaliana]
gi|25288972|pir||A84807 probable acid phosphatase
[imported] - Arabidopsis thaliana
gi|15224907|ref|NP_181394.1| acid phosphatase class B
family protein [Arabidopsis thaliana]
Length = 251
Score = 40.0 bits (92), Expect = 0.012
Identities = 25/75 (33%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Query: 60 NNDYSYCKIHSLHAKLNNLEEHN-VPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGV 118
N SYC L + NN+ VP C Y+ GQY RD+ T I+ Y N +
Sbjct: 32 NYGASYCLSWRLAVETNNVRAWRIVPLQCLRYVEVYMLAGQYDRDVQLTVDQIKVYLNEI 91
Query: 119 KPSEDGFDVVLIDID 133
DG D ++D+D
Sbjct: 92 ILPGDGMDAWILDVD 106
>emb|CAA39370.1| acid phosphatase [Lycopersicon esculentum] gi|7435103|pir||T06587
acid phosphatase (EC 3.1.3.2) 1 - tomato
gi|130718|sp|P27061|PPA1_LYCES Acid phosphatase 1
precursor (Apase-1(1)) gi|170372|gb|AAA34135.1| acid
phosphatase type 5 gi|170370|gb|AAA34134.1| acid
phosphatase type 1 gi|445121|prf||1908427A acid
phosphatase 1
Length = 255
Score = 39.7 bits (91), Expect = 0.015
Identities = 22/81 (27%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Query: 66 CKIHSLHAKLNNLEE-HNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDG 124
C + NNL +P C D +Y+ G Y ++D +Y V +DG
Sbjct: 43 CTTWRFVVETNNLSPWKTIPEECADYVKEYMVGPGYKMEIDRVSDEAGEYAKSVDLGDDG 102
Query: 125 FDVVLIDIDSLFQWNPPHSSN 145
DV + D+D N P+ S+
Sbjct: 103 RDVWIFDVDETLLSNLPYYSD 123
>prf||1908418A acid phosphatase 1
Length = 255
Score = 39.7 bits (91), Expect = 0.015
Identities = 22/81 (27%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Query: 66 CKIHSLHAKLNNLEE-HNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDG 124
C + NNL +P C D +Y+ G Y ++D +Y V +DG
Sbjct: 43 CTTWRFVVETNNLSPWKTIPEECADYVKEYMVGPGYKMEIDRVSDEAGEYAKSVDLGDDG 102
Query: 125 FDVVLIDIDSLFQWNPPHSSN 145
DV + D+D N P+ S+
Sbjct: 103 RDVWIFDVDETLLSNLPYYSD 123
>ref|XP_479551.1| putative syringolide-induced protein [Oryza sativa (japonica
cultivar-group)] gi|33146665|dbj|BAC80011.1| putative
syringolide-induced protein [Oryza sativa (japonica
cultivar-group)]
Length = 244
Score = 38.1 bits (87), Expect = 0.044
Identities = 22/73 (30%), Positives = 32/73 (43%), Gaps = 1/73 (1%)
Query: 62 DYSYCKIHSLHAKLNNLEEH-NVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKP 120
D YC + + NN + VP C +Y+ GQYARD+ I Y +
Sbjct: 29 DEPYCLSWRVMVEANNAKNWPTVPPPCVGYVWRYMAWGQYARDVAGVADQIAAYAAQLAA 88
Query: 121 SEDGFDVVLIDID 133
+DG D + D+D
Sbjct: 89 GDDGLDAWVFDVD 101
>dbj|BAD95053.1| acid phosphatase [Arabidopsis thaliana] gi|8843863|dbj|BAA97389.1|
acid phosphatase [Arabidopsis thaliana]
gi|15241412|ref|NP_199939.1| acid phosphatase, putative
[Arabidopsis thaliana]
Length = 257
Score = 38.1 bits (87), Expect = 0.044
Identities = 22/79 (27%), Positives = 34/79 (42%), Gaps = 1/79 (1%)
Query: 65 YCKIHSLHAKLNNLEE-HNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSED 123
+C A++NNL +P C D Y+ G Y DL+ + ++ S D
Sbjct: 44 HCTTWRFAAEMNNLAPWKTIPVECADYVKDYVMGKGYLTDLERVSEEALIFARSIEFSGD 103
Query: 124 GFDVVLIDIDSLFQWNPPH 142
G D+ + DID N P+
Sbjct: 104 GKDIWIFDIDETLLSNLPY 122
>dbj|BAB86895.1| syringolide-induced protein B15-3-5 [Glycine max]
Length = 234
Score = 37.4 bits (85), Expect = 0.075
Identities = 22/71 (30%), Positives = 34/71 (46%), Gaps = 1/71 (1%)
Query: 64 SYCKIHSLHAKLNNLEEHN-VPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSE 122
SY + L + NN VP+ C + Y+ GGQY DL+ I Y + + +
Sbjct: 19 SYGRSWRLTVEANNARPWRIVPDNCYNHLQNYMSGGQYQLDLNLVVQHILSYAHEIPLAA 78
Query: 123 DGFDVVLIDID 133
DG D ++D+D
Sbjct: 79 DGMDAWILDVD 89
>ref|NP_765042.1| replication-associated protein [Staphylococcus epidermidis ATCC
12228] gi|27315952|gb|AAO05086.1| replication-associated
protein [Staphylococcus epidermidis ATCC 12228]
Length = 285
Score = 35.8 bits (81), Expect = 0.22
Identities = 33/137 (24%), Positives = 61/137 (44%), Gaps = 17/137 (12%)
Query: 8 ESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQN-------SNGGIIELRNIN 60
++G Y+ + AT + L+ + V M L + SN I+E++N+
Sbjct: 76 DNGLYVLKYKATSTANKTVVVNSELLNVNVFMEKGLTKLNDVKVFYPLSNYFIVEIKNLP 135
Query: 61 NDYSYCKIHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKP 120
DY KI + KL + E+ NV + K+++ QY + RD + +Y+
Sbjct: 136 KDYKALKI---NFKLLDKEDENVSSNSKEMS-QYTSAEKNNRDYNLQPKTKSEYY----- 186
Query: 121 SEDGFDVVLIDIDSLFQ 137
D F + L ++DS ++
Sbjct: 187 -VDAFQLRLKELDSKYK 202
>ref|ZP_00323306.1| COG0657: Esterase/lipase [Pediococcus pentosaceus ATCC 25745]
Length = 259
Score = 35.4 bits (80), Expect = 0.28
Identities = 25/89 (28%), Positives = 44/89 (49%), Gaps = 14/89 (15%)
Query: 59 INNDYSYCKIHSLHAKLNNLEEHNVPNICKDLALQYIKGGQY----ARDLDSTKSVIEDY 114
I ND Y +IH+L A L + N K L + GG + A+D D +++++D
Sbjct: 3 IKNDLIYDEIHALKADL-----YQPDNASKGLIIDIHGGGWFRGDKAKDQDLAEALVKDG 57
Query: 115 FNGVKPS-----EDGFDVVLIDIDSLFQW 138
F+ + P+ + + L D+D+L+ W
Sbjct: 58 FSVIVPNYRLTPDAYYPAPLDDMDTLYHW 86
>gb|AAC60539.2| acid phosphatase-1(1); Apase-1(1) [Lycopersicon esculentum]
Length = 174
Score = 35.0 bits (79), Expect = 0.37
Identities = 20/71 (28%), Positives = 32/71 (44%), Gaps = 1/71 (1%)
Query: 76 NNLEE-HNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDS 134
NNL +P D +Y+ G Y ++D +Y V +DG DV + D+D
Sbjct: 6 NNLSPGKTIPEEGADYVKEYMVGPGYKMEIDRVSDEAGEYAKSVDLGDDGRDVWIFDVDE 65
Query: 135 LFQWNPPHSSN 145
+ N P+ S+
Sbjct: 66 TWLSNLPYYSD 76
>ref|NP_376216.1| hypothetical spermidine synthase [Sulfolobus tokodaii str. 7]
gi|15621330|dbj|BAB65325.1| 300aa long hypothetical
spermidine synthase [Sulfolobus tokodaii str. 7]
gi|45477329|sp|Q975S5|SPEE_SULTO Probable spermidine
synthase (Putrescine aminopropyltransferase) (SPDSY)
Length = 300
Score = 33.9 bits (76), Expect = 0.83
Identities = 20/70 (28%), Positives = 38/70 (53%), Gaps = 1/70 (1%)
Query: 72 HAKLNNLEEHNVPNICKDLALQYIKGG-QYARDLDSTKSVIEDYFNGVKPSEDGFDVVLI 130
H + N+ ++ + D A +Y++ Q A D +K VIED + +K +++ FD V+I
Sbjct: 98 HKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVI 157
Query: 131 DIDSLFQWNP 140
D+ + +P
Sbjct: 158 DLTDPIKDSP 167
>gb|EAA20715.1| hypothetical protein [Plasmodium yoelii yoelii]
Length = 702
Score = 33.9 bits (76), Expect = 0.83
Identities = 23/84 (27%), Positives = 37/84 (43%), Gaps = 1/84 (1%)
Query: 43 LQSCQNSNGGIIELRNINNDYSYCKIHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYAR 102
L+ C N + I N YCK H K +N+ +H P + K L+ G QY R
Sbjct: 149 LKLCNNLDMSFIYNTYDKNGLLYCKKIKFHVKGDNINDHITPLVVKINTLKNNMGKQYMR 208
Query: 103 DLDSTKSVIEDYFNGVKPSEDGFD 126
L+ + E +F + E+ ++
Sbjct: 209 -LELSDDKNESFFYYLDLFEENYE 231
>ref|NP_473276.1| hypothetical protein [Plasmodium falciparum 3D7]
gi|3758862|emb|CAB11147.1| hypothetical protein
[Plasmodium falciparum 3D7] gi|7494241|pir||T18508
hypothetical protein C0715c - malaria parasite
(Plasmodium falciparum)
Length = 1008
Score = 33.5 bits (75), Expect = 1.1
Identities = 32/108 (29%), Positives = 49/108 (44%), Gaps = 27/108 (25%)
Query: 48 NSNGGIIELRN----INND--YSYCKIHSLHAKLNNLEEHN-----VPNICKDLAL---- 92
N GII+ INND Y++ IH+ NN++E+N +PN D L
Sbjct: 164 NKTEGIIDKNKSSNVINNDSEYNHPNIHNNEGTYNNIDENNPMYEGMPNHLSDSNLESAM 223
Query: 93 ----QYIKGGQ----YARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDI 132
+YIKG + R+ + K ++E +K ED F L+D+
Sbjct: 224 KKAEEYIKGKEDDIIERREKEKKKKIVEK----MKDDEDVFTDKLLDL 267
>emb|CAD62799.1| amino acid transport protein [Lactobacillus plantarum WCFS1]
gi|28377069|ref|NP_783961.1| amino acid transport
protein [Lactobacillus plantarum WCFS1]
Length = 454
Score = 33.5 bits (75), Expect = 1.1
Identities = 21/86 (24%), Positives = 39/86 (44%), Gaps = 4/86 (4%)
Query: 1 MGSSFVLESGFYITSFS--ATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNGGIIELRN 58
+G+ FV+ S + S + F+ FA +G+ +++ ++ S +N GI
Sbjct: 242 VGAIFVIVSIYPWDKLSQIGSPFVQTFAKIGITAAASIINFVVITASLSGTNSGIYSASR 301
Query: 59 INNDYSYCKIHSLHAKLNNLEEHNVP 84
+ Y+ + H L K+ L H VP
Sbjct: 302 MT--YTLAESHQLPRKMTLLNRHGVP 325
>ref|YP_176402.1| D-alanine--D-alanine ligase A [Bacillus clausii KSM-K16]
gi|56910914|dbj|BAD65441.1| D-alanine--D-alanine ligase
A [Bacillus clausii KSM-K16]
Length = 368
Score = 33.1 bits (74), Expect = 1.4
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 6/53 (11%)
Query: 96 KGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDSLFQWNPPHSSNLLL 148
K ++ L S K+++E +D FDV LI ID QW+ +SN LL
Sbjct: 13 KSAEHEVSLQSAKNIVEAI------DKDRFDVTLIGIDKQGQWHINEASNFLL 59
>dbj|BAC82457.1| pod storage protein [Phaseolus vulgaris] gi|7442055|pir||T11761 pod
storage protein - kidney bean gi|1816410|dbj|BAA19152.1|
pod storage protein [Phaseolus vulgaris]
Length = 255
Score = 33.1 bits (74), Expect = 1.4
Identities = 21/62 (33%), Positives = 27/62 (42%), Gaps = 2/62 (3%)
Query: 83 VPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDSLFQWNPPH 142
+P C D YI+GGQY D + I YF DV+L +ID N P+
Sbjct: 62 IPQQCVDATANYIEGGQYRSDSKTVNQQI--YFFARDRHVHENDVILFNIDGTALSNIPY 119
Query: 143 SS 144
S
Sbjct: 120 YS 121
>gb|AAL17638.1| putative defense associated acid phosphatase [Phaseolus vulgaris]
Length = 264
Score = 33.1 bits (74), Expect = 1.4
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 1/59 (1%)
Query: 76 NNLEE-HNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDID 133
NN+ VP C Y+ GQY RDL I Y + + S DG D ++D+D
Sbjct: 61 NNVRRWRTVPPQCYHHLQNYMCAGQYERDLSLAVEHILLYASQIPLSPDGMDAWILDVD 119
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.321 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 253,689,661
Number of Sequences: 2540612
Number of extensions: 10075528
Number of successful extensions: 27044
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 32
Number of HSP's that attempted gapping in prelim test: 27009
Number of HSP's gapped (non-prelim): 65
length of query: 149
length of database: 863,360,394
effective HSP length: 125
effective length of query: 24
effective length of database: 545,783,894
effective search space: 13098813456
effective search space used: 13098813456
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 67 (30.4 bits)
Medicago: description of AC147429.11