BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146743.14 + phase: 0
(720 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_181337.2| ceramidase family protein [Arabidopsis thaliana] 841 0.0
ref|NP_917762.1| P0501G01.24 [Oryza sativa (japonica cultivar-gr... 832 0.0
ref|NP_172218.1| ceramidase family protein [Arabidopsis thaliana] 813 0.0
gb|AAF79556.1| F22G5.28 [Arabidopsis thaliana] gi|25333800|pir||... 800 0.0
ref|NP_200706.1| ceramidase family protein [Arabidopsis thaliana] 783 0.0
gb|AAD32770.1| hypothetical protein [Arabidopsis thaliana] gi|25... 756 0.0
dbj|BAB09641.1| neutral ceramidase [Arabidopsis thaliana] 701 0.0
ref|XP_647372.1| hypothetical protein DDB0202169 [Dictyostelium ... 552 e-155
ref|NP_973628.1| ceramidase family protein [Arabidopsis thaliana] 541 e-152
dbj|BAD69590.1| neutral ceramidase [Danio rerio] gi|56090224|ref... 527 e-148
gb|AAQ04667.1| neutral/alkaline ceramidase [Homo sapiens] 516 e-144
gb|AAL06061.1| mitochondrial ceramidase [Homo sapiens] gi|984526... 516 e-144
dbj|BAC92751.1| ceramidase [Dictyostelium discoideum] gi|6680020... 516 e-144
gb|AAB69633.1| random slug cDNA25 protein [Dictyostelium discoid... 516 e-144
emb|CAI15870.1| N-acylsphingosine amidohydrolase (non-lysosomal ... 515 e-144
ref|XP_309165.2| ENSANGP00000018598 [Anopheles gambiae str. PEST... 514 e-144
ref|NP_061300.1| N-acylsphingosine amidohydrolase 2 [Mus musculu... 504 e-141
emb|CAF92687.1| unnamed protein product [Tetraodon nigroviridis] 504 e-141
dbj|BAC38089.1| unnamed protein product [Mus musculus] 504 e-141
ref|NP_446098.1| N-acylsphingosine amidohydrolase 2 [Rattus norv... 501 e-140
>ref|NP_181337.2| ceramidase family protein [Arabidopsis thaliana]
Length = 757
Score = 841 bits (2172), Expect = 0.0
Identities = 436/744 (58%), Positives = 531/744 (70%), Gaps = 49/744 (6%)
Query: 19 MQSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFV 78
+ T YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIVAE QG R V
Sbjct: 19 LSRTVYAYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVV 77
Query: 79 FVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLG 138
FVNLDA MASQ++TIK+LERLK+R+G LYTE+NVAISGIHTHAGPGGYLQYV Y VTSLG
Sbjct: 78 FVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLG 137
Query: 139 FVTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYP 198
FV QSFD + N +EQSI+QAH +L+PGS F+N GD+ +A +NRSPS+YL NPA ERS+Y
Sbjct: 138 FVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYK 197
Query: 199 SNVDTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIG----------- 247
+VD +MTL+KFVDS G + GSF+WFATHGTSMS N LISGDNK
Sbjct: 198 YDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENG 256
Query: 248 --------------------------ELVQIAQLIKATGGKDCNEKSSQASKVRKNDGSL 281
L+ IA K++ G ++ ++VR
Sbjct: 257 QKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRK 316
Query: 282 FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEILSTKII 341
FV AFCQSN GDVSPN LG FCID+G PCDFNHS+CNG + LC GRGPGYP+E ST+II
Sbjct: 317 FVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRII 376
Query: 342 GERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDN----KKVVKTCPAALGPGF 397
GE+QF+ AVELF A+E+L GKI Y+H YL+F+N++V + + VKTCPAA+G GF
Sbjct: 377 GEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGF 436
Query: 398 AAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPW 457
AAGTTDGPG F F+QGD + + FW+ VR+ L+ P QV CQ PKP+LL +GEM +PY W
Sbjct: 437 AAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDW 496
Query: 458 APAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHVVIAGLT 517
AP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K LIS+ EF+N HVVIAGLT
Sbjct: 497 APSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLT 556
Query: 518 NTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNGTSPPDL 577
NTYSQYIATFEEY QRYE ASTLYG HTL+AYIQEF KLA A+ G + G PPDL
Sbjct: 557 NTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTL-PRGPQPPDL 615
Query: 578 LSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDKPSATFWSANPRYDLLTEG 637
L Q S L D+TP G+K GD+K D+ P F +G + +ATFWS PR DL+TEG
Sbjct: 616 LDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRGQQVNATFWSGCPRNDLMTEG 673
Query: 638 TYAVVERL-QGERWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAISGVYRLKH 695
++AVVE L +G +W V DDDD SL F+W + S A IEW +P A++GVYR++H
Sbjct: 674 SFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRH 733
Query: 696 FGASKKTIVSPINYFTGASSAFAV 719
+GAS K++ I+ F+G+SSAF V
Sbjct: 734 YGAS-KSLFGSISSFSGSSSAFVV 756
>ref|NP_917762.1| P0501G01.24 [Oryza sativa (japonica cultivar-group)]
Length = 818
Score = 832 bits (2150), Expect = 0.0
Identities = 431/751 (57%), Positives = 533/751 (70%), Gaps = 64/751 (8%)
Query: 26 YLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAG 85
YL+G+GS+D+TGPAADVNMMGYAN EQ +GIHFRL++R FIVAE G R VFVN+DA
Sbjct: 41 YLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRAFIVAEP-NGKRVVFVNIDAC 99
Query: 86 MASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQSFD 145
MASQ++TIK+LERLK+R+G+LY E NVAISGIHTHAGPGGYLQYVVY VTSLGFV QSFD
Sbjct: 100 MASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFD 159
Query: 146 AIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQM 205
I + +EQSI++AHNNL+PG IF+N GD+ +A +NRSPSAYL NPAEERS+Y NVD +M
Sbjct: 160 VIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSPSAYLNNPAEERSKYEYNVDKEM 219
Query: 206 TLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIG------------------ 247
TL+KFVD G GSF+WFATHGTSMS N LISGDNK
Sbjct: 220 TLIKFVDDELGPV-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSA 278
Query: 248 --------------------------------ELVQIAQLIKATGGKDCNEKS-SQASKV 274
+L+Q+A +A+GG+ S ++ +
Sbjct: 279 HANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASSYEASGGRRLAGSSITRRIRS 338
Query: 275 RKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNE 334
+ + FV AFCQSN GDVSPNVLG FCID+ PCDFNHS+CNG + LC GRGPGYP+E
Sbjct: 339 TQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDFNHSTCNGKNELCYGRGPGYPDE 398
Query: 335 ILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDN----KKVVKTCP 390
ST++IG RQF A +LF SASEE+ GKIDYRH YL+F+ +EV++ ++ VKTCP
Sbjct: 399 FESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTVKTCP 458
Query: 391 AALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGE 450
AA+G FAAGTTDGPG F F+QGD + +PFWK VR+ LK P + QV+C +PKP+LL +GE
Sbjct: 459 AAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGE 518
Query: 451 MFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETH 510
M +PY WAPAILP+Q++R+G+L+IL VPGEFTTMAGRRLR+AVK T++++ N EF+ H
Sbjct: 519 MKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVK-TVLTSGNSEFDKNIH 577
Query: 511 VVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGN 570
VV+AGLTN+YSQYI TFEEY QRYE ASTLYGPHTLSAYIQEF KLA AM ++ N
Sbjct: 578 VVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTN 637
Query: 571 GTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDKPSATFWSANPR 630
PPD+L Q L D+TP G+K GD+ D+ PG+ F KG +ATF+SA PR
Sbjct: 638 -FQPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDV--PGNSTFNKGSTVNATFYSACPR 694
Query: 631 YDLLTEGTYAVVERLQG-ERWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAIS 688
DLLT+GT+A+VE+L G W+ V DDDD SL F+W + S FA +EW +P DA +
Sbjct: 695 NDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAA 754
Query: 689 GVYRLKHFGASKKTIVSPINYFTGASSAFAV 719
GVYRL+HFGASK S + +FTG S AFA+
Sbjct: 755 GVYRLRHFGASKPMFGS-VRHFTGTSRAFAL 784
>ref|NP_172218.1| ceramidase family protein [Arabidopsis thaliana]
Length = 779
Score = 813 bits (2101), Expect = 0.0
Identities = 429/752 (57%), Positives = 527/752 (70%), Gaps = 60/752 (7%)
Query: 22 TYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVN 81
++ EYLIG+GSYD+TGPAADVNMMGYAN+EQ +GIHFRLRARTFIV+E QG R VFVN
Sbjct: 33 SHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVN 91
Query: 82 LDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVT 141
LDA MASQ++ +K++ERLK+R+G+LYTE+NV ISGIHTHAGPGGYLQYVVY VTSLGFV
Sbjct: 92 LDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVR 151
Query: 142 QSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNV 201
QSFDA+ + +E SIIQAH NL+PGSIF+N G++ +A +NRSPSAYL NP++ERS++ NV
Sbjct: 152 QSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNV 211
Query: 202 DTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIG-------------- 247
D +MTLLKFVD G GSF+WFATHGTSMS N LISGDNK
Sbjct: 212 DKEMTLLKFVDDQWGPV-GSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAE 270
Query: 248 ----------------------------ELVQIAQLIKATGGKDCNEKSSQASKVR---- 275
EL+++A ++ GK SS A +VR
Sbjct: 271 RSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALR 330
Query: 276 KNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEI 335
K D FV AFCQ+N GDVSPNVLGAFC+D+G PCDFNHS+C G + +C GRGPGYP+E
Sbjct: 331 KADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEF 390
Query: 336 LSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNK----KVVKTCPA 391
ST+IIGERQF+ A+ELF ASE+L GK+DYRHVY++F+ + V L K +VVKTCPA
Sbjct: 391 ESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPA 450
Query: 392 ALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEM 451
A+G FAAGTTDGPG F F QGD + +PFW+ VR+ LK P + Q+DC PKP+LL +GEM
Sbjct: 451 AMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEM 510
Query: 452 FDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHV 511
PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK L ++ N + + E HV
Sbjct: 511 TKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHV 570
Query: 512 VIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNG 571
VIAGL N YSQY+ TFEEY QRYE ASTLYGPHTLS YIQEF KL++++ D G
Sbjct: 571 VIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVL-DMPVQPG 629
Query: 572 TSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGD-KPSATFWSANPR 630
PPDLL Q SFL DTTP G GD+ D+ P + +G+ + + F SA PR
Sbjct: 630 PQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQVTVVFRSACPR 687
Query: 631 YDLLTEGTYAVVERLQ--GERWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAI 687
DLLTEGT+ +VERL+ + W V DDDDL L F+W + S A +EW IP A
Sbjct: 688 NDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESAS 747
Query: 688 SGVYRLKHFGASKKTIVSPINYFTGASSAFAV 719
GVYR+ HFGA+KK + +++FTG+SSAF V
Sbjct: 748 PGVYRITHFGAAKK-LFGSVHHFTGSSSAFVV 778
>gb|AAF79556.1| F22G5.28 [Arabidopsis thaliana] gi|25333800|pir||G86208 protein
F22G5.28 [imported] - Arabidopsis thaliana
Length = 808
Score = 800 bits (2067), Expect = 0.0
Identities = 421/734 (57%), Positives = 514/734 (69%), Gaps = 59/734 (8%)
Query: 22 TYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVN 81
++ EYLIG+GSYD+TGPAADVNMMGYAN+EQ +GIHFRLRARTFIV+E QG R VFVN
Sbjct: 34 SHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVN 92
Query: 82 LDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVT 141
LDA MASQ++ +K++ERLK+R+G+LYTE+NV ISGIHTHAGPGGYLQYVVY VTSLGFV
Sbjct: 93 LDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVR 152
Query: 142 QSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNV 201
QSFDA+ + +E SIIQAH NL+PGSIF+N G++ +A +NRSPSAYL NP++ERS++ NV
Sbjct: 153 QSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNV 212
Query: 202 DTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIG-------------- 247
D +MTLLKFVD G GSF+WFATHGTSMS N LISGDNK
Sbjct: 213 DKEMTLLKFVDDQWGPV-GSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAE 271
Query: 248 ----------------------------ELVQIAQLIKATGGKDCNEKSSQASKVR---- 275
EL+++A ++ GK SS A +VR
Sbjct: 272 RSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALR 331
Query: 276 KNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEI 335
K D FV AFCQ+N GDVSPNVLGAFC+D+G PCDFNHS+C G + +C GRGPGYP+E
Sbjct: 332 KADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEF 391
Query: 336 LSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNK----KVVKTCPA 391
ST+IIGERQF+ A+ELF ASE+L GK+DYRHVY++F+ + V L K +VVKTCPA
Sbjct: 392 ESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPA 451
Query: 392 ALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEM 451
A+G FAAGTTDGPG F F QGD + +PFW+ VR+ LK P + Q+DC PKP+LL +GEM
Sbjct: 452 AMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEM 511
Query: 452 FDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHV 511
PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK L ++ N + + E HV
Sbjct: 512 TKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHV 571
Query: 512 VIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNG 571
VIAGL N YSQY+ TFEEY QRYE ASTLYGPHTLS YIQEF KL++++ D G
Sbjct: 572 VIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVL-DMPVQPG 630
Query: 572 TSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGD-KPSATFWSANPR 630
PPDLL Q SFL DTTP G GD+ D+ P + +G+ + + F SA PR
Sbjct: 631 PQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQVTVVFRSACPR 688
Query: 631 YDLLTEGTYAVVERLQ--GERWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAI 687
DLLTEGT+ +VERL+ + W V DDDDL L F+W + S A +EW IP A
Sbjct: 689 NDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESAS 748
Query: 688 SGVYRLKHFGASKK 701
GVYR+ HFGA+KK
Sbjct: 749 PGVYRITHFGAAKK 762
>ref|NP_200706.1| ceramidase family protein [Arabidopsis thaliana]
Length = 733
Score = 783 bits (2023), Expect = 0.0
Identities = 422/732 (57%), Positives = 518/732 (70%), Gaps = 31/732 (4%)
Query: 6 IITVLGLVCFWSWMQSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRART 65
++ +L L C +S +YL+G+GSYD+TGPAADVNMMGYAN+EQ +G+HFRLRAR
Sbjct: 14 LLFLLRLTCIFS-----DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARA 68
Query: 66 FIVAENLQGPRFVFVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGG 125
FIVAE + R FVNLDAGMASQL+TIK++ERLK R+G LYTEENVAISG HTHAGPGG
Sbjct: 69 FIVAEPYK-KRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGG 127
Query: 126 YLQYVVYSVTSLGFVTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSA 185
YLQY++Y VTSLGFV QSF+A+ + +EQSIIQAH NL+PGSI IN G++ +A +NRSPSA
Sbjct: 128 YLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSA 187
Query: 186 YLLNPAEERSRYPSNVDTQMTLLKFVDSASGK-SKGSFSWFATHGTSMS---NNNKLISG 241
YL NPA ERS+Y +VD +MTL+KFVD G ++ WF S + + +S
Sbjct: 188 YLNNPAHERSKYEYDVDKEMTLVKFVDDQWGPVARIMEDWFERENGCRSVDVESPRRVSS 247
Query: 242 DNKD--IGELVQIAQLIKATGGKDCNEKSSQASKVRKN----DGSLFVGAFCQSNVGDVS 295
D +L+++A + +TGGK SS A +VR D FV AFCQ+N GDVS
Sbjct: 248 IISDPYDQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVS 307
Query: 296 PNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGS 355
PNVLGAFCID+G PC+FN S+C G + C GRGPGYP+E ST+IIGERQF+ A +LF
Sbjct: 308 PNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTK 367
Query: 356 ASEELTGKIDYRHVYLNFTNIEVELDNK----KVVKTCPAALGPGFAAGTTDGPGVFGFQ 411
ASEE+ GK+DYRH Y++F+ +EV ++ + +VVKTCPAA+G GFAAGTTDGPG F F+
Sbjct: 368 ASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFK 427
Query: 412 QGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGK 471
QGD + +PFW+ VR+ LK P++ QV CQ PKP+LL +GEM PY WAP+ILP+QILR+G+
Sbjct: 428 QGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQ 487
Query: 472 LIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYH 531
L+IL VPGEFTTMAGRRLR+AVK L SNG E VVIAGLTN+YSQYIATFEEY
Sbjct: 488 LVILCVPGEFTTMAGRRLRDAVKTVLKEGSNG---REFSVVIAGLTNSYSQYIATFEEYQ 544
Query: 532 QQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGD 591
QRYE ASTLYGPHTLS YIQEF KLA + + G PPDLL Q S L D
Sbjct: 545 VQRYEGASTLYGPHTLSGYIQEFKKLANDLLSA-QTTDPGPQPPDLLHKQISLLTPVVAD 603
Query: 592 TTPDGIKLGDIKEDIAFPGSGYFTKG-DKPSATFWSANPRYDLLTEGTYAVVER-LQG-E 648
TP G GD+ D+ P F KG D F SANPR DL+TEGT+A+VER L+G E
Sbjct: 604 MTPIGTAFGDVTSDV--PRLSKFRKGADIVRVQFRSANPRNDLMTEGTFALVERWLEGRE 661
Query: 649 RWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAISGVYRLKHFGASKKTIVSPI 707
W+ V DDDD L F+W + S A IEW IP A GVYR+ HFG S KT +S I
Sbjct: 662 TWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFG-SAKTPISSI 720
Query: 708 NYFTGASSAFAV 719
++F+G+SSAF V
Sbjct: 721 HHFSGSSSAFVV 732
>gb|AAD32770.1| hypothetical protein [Arabidopsis thaliana] gi|25333797|pir||H84799
hypothetical protein At2g38010 [imported] - Arabidopsis
thaliana
Length = 715
Score = 756 bits (1952), Expect = 0.0
Identities = 403/744 (54%), Positives = 492/744 (65%), Gaps = 91/744 (12%)
Query: 19 MQSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFV 78
+ T YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIVAE
Sbjct: 19 LSRTVYAYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP------- 71
Query: 79 FVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLG 138
E+NVAISGIHTHAGPGGYLQYV Y VTSLG
Sbjct: 72 -----------------------------QEKNVAISGIHTHAGPGGYLQYVTYIVTSLG 102
Query: 139 FVTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYP 198
FV QSFD + N +EQSI+QAH +L+PGS F+N GD+ +A +NRSPS+YL NPA ERS+Y
Sbjct: 103 FVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYK 162
Query: 199 SNVDTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIG----------- 247
+VD +MTL+KFVDS G + GSF+WFATHGTSMS N LISGDNK
Sbjct: 163 YDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENG 221
Query: 248 --------------------------ELVQIAQLIKATGGKDCNEKSSQASKVRKNDGSL 281
L+ IA K++ G ++ ++VR
Sbjct: 222 QKNSVSSRNIPRRVSTIVSDFSRNQSRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRK 281
Query: 282 FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEILSTKII 341
FV AFCQSN GDVSPN LG FCID+G PCDFNHS+CNG + LC GRGPGYP+E ST+II
Sbjct: 282 FVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRII 341
Query: 342 GERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVEL----DNKKVVKTCPAALGPGF 397
GE+QF+ AVELF A+E+L GKI Y+H YL+F+N++V + + VKTCPAA+G GF
Sbjct: 342 GEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGF 401
Query: 398 AAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPW 457
AAGTTDGPG F F+QGD + + FW+ VR+ L+ P QV CQ PKP+LL +GEM +PY W
Sbjct: 402 AAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDW 461
Query: 458 APAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHVVIAGLT 517
A ILR+G+L+ILSVPGEFTTMAGRRLR+A+K LIS+ EF+N HVVIAGLT
Sbjct: 462 A-------ILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLT 514
Query: 518 NTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNGTSPPDL 577
NTYSQYIATFEEY QRYE ASTLYG HTL+AYIQEF KLA A+ G + G PPDL
Sbjct: 515 NTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTL-PRGPQPPDL 573
Query: 578 LSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDKPSATFWSANPRYDLLTEG 637
L Q S L D+TP G+K GD+K D+ P F +G + +ATFWS PR DL+TEG
Sbjct: 574 LDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRGQQVNATFWSGCPRNDLMTEG 631
Query: 638 TYAVVERL-QGERWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAISGVYRLKH 695
++AVVE L +G +W V DDDD SL F+W + S A IEW +P A++GVYR++H
Sbjct: 632 SFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRH 691
Query: 696 FGASKKTIVSPINYFTGASSAFAV 719
+GAS K++ I+ F+G+SSAF V
Sbjct: 692 YGAS-KSLFGSISSFSGSSSAFVV 714
>dbj|BAB09641.1| neutral ceramidase [Arabidopsis thaliana]
Length = 705
Score = 701 bits (1809), Expect = 0.0
Identities = 393/746 (52%), Positives = 485/746 (64%), Gaps = 87/746 (11%)
Query: 6 IITVLGLVCFWSWMQSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRART 65
++ +L L C +S +YL+G+GSYD+TGPAADVNMMGYAN+EQ +G+HFRLRAR
Sbjct: 14 LLFLLRLTCIFS-----DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARA 68
Query: 66 FIVAENLQGPRFVFVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGG 125
FIVAE + ENVAISG HTHAGPGG
Sbjct: 69 FIVAEPYK------------------------------------ENVAISGTHTHAGPGG 92
Query: 126 YLQYVVYSVTSLGFVTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSA 185
YLQY++Y VTSLGFV QSF+A+ + +EQSIIQAH NL+PGSI IN G++ +A +NRSPSA
Sbjct: 93 YLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSA 152
Query: 186 YLLNPAEERSRYPSNVDTQMTLLKFVDSA--------SGKSKGSFS-----WFATHGTSM 232
YL NPA ERS+Y +VD +MTL+KFVD G +KG+ + WF
Sbjct: 153 YLNNPAHERSKYEYDVDKEMTLVKFVDDQWGPVGSFNCGDNKGTAARIMEDWFERENGCR 212
Query: 233 SNN-------NKLISGDNKDIGELVQIAQLIKATGGKDCNEKSSQASKVRKN----DGSL 281
S + + +IS + +L+++A + +TGGK SS A +VR D
Sbjct: 213 SVDVESPRRVSSIISDPYGEHQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPR 272
Query: 282 FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEILSTKII 341
FV AFCQ+N GDVSPNVLGAFCID+G PC+FN S+C G + C GRGPGYP+E ST+II
Sbjct: 273 FVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRII 332
Query: 342 GERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNK----KVVKTCPAALGPGF 397
GERQF+ A +LF ASEE+ GK+DYRH Y++F+ +EV ++ + +VVKTCPAA+G GF
Sbjct: 333 GERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGF 392
Query: 398 AAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPW 457
AAGTTDGPG F F+QGD + +PFW+ VR+ LK P++ QV CQ PKP+LL +GEM PY W
Sbjct: 393 AAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDW 452
Query: 458 APAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHVVIAGLT 517
A ILR+G+L+IL VPGEFTTMAGRRLR+AVK L SNG E VVIAGLT
Sbjct: 453 A-------ILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNG---REFSVVIAGLT 502
Query: 518 NTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNGTSPPDL 577
N+YSQYIATFEEY QRYE ASTLYGPHTLS YIQEF KLA + + G PPDL
Sbjct: 503 NSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSA-QTTDPGPQPPDL 561
Query: 578 LSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKG-DKPSATFWSANPRYDLLTE 636
L Q S L D TP G GD+ D+ P F KG D F SANPR DL+TE
Sbjct: 562 LHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKGADIVRVQFRSANPRNDLMTE 619
Query: 637 GTYAVVER-LQG-ERWISVQDDDDLSLFFRW-KVDNTSFHGFAAIEWEIPTDAISGVYRL 693
GT+A+VER L+G E W+ V DDDD L F+W + S A IEW IP A GVYR+
Sbjct: 620 GTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRI 679
Query: 694 KHFGASKKTIVSPINYFTGASSAFAV 719
HFG S KT +S I++F+G+SSAF V
Sbjct: 680 THFG-SAKTPISSIHHFSGSSSAFVV 704
>ref|XP_647372.1| hypothetical protein DDB0202169 [Dictyostelium discoideum]
gi|60475705|gb|EAL73640.1| hypothetical protein
DDB0202169 [Dictyostelium discoideum]
Length = 718
Score = 552 bits (1423), Expect = e-155
Identities = 311/708 (43%), Positives = 428/708 (59%), Gaps = 48/708 (6%)
Query: 26 YLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAG 85
Y IG G YD+TG +A+VN+MGYAN Q AGIHFR RAR F+ ++ G R V+V+ D+
Sbjct: 46 YQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDS-NGNRAVYVSTDSC 104
Query: 86 MASQLLTIKLLERLKSRFG-NLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQSF 144
M Q + I ++E L+ FG N+YTE NV +SG HTH+GP G+ QY +Y +TSLGF ++F
Sbjct: 105 MIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYKKNF 164
Query: 145 DAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQ 204
D I N + Q+I++AH +++P ++F TG++ +INRSP AY NP EE++ Y SNVD
Sbjct: 165 DTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNVDKN 224
Query: 205 MTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLI-KATGGKD 263
MT+L+ ++ +G + S+FA H TSM+N N LISGDNK + Q+ T GK
Sbjct: 225 MTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWEKQVNGPGTAGK- 282
Query: 264 CNEKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLL 323
FV AF QSN GDVSPN G C D G PCD+ S+CNG +
Sbjct: 283 ----------------GPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEE 325
Query: 324 CVGRGPGYPNEIL-STKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVE--- 379
C GPG ++ ST+IIG QF A+ELF +AS +++G + YRH ++ FTN+ VE
Sbjct: 326 CWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPY 385
Query: 380 ---LDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEIS--PFWKNVRDFLKEPSQY 434
+DN TC A+G FAAGTTDGPG F F Q D S PFW + DF+ +P+
Sbjct: 386 NSGVDN---ATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPD 442
Query: 435 QVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVK 494
Q+ CQ+PKP+LL G M +P PW P ++PIQI+ +G++++++VPGEFTTM+GRRLR +V+
Sbjct: 443 QIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVR 501
Query: 495 ETLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEF 554
E + GE V+IAGL+NTYS YIATFEEY QRYE AST++GPHTL +Y+QEF
Sbjct: 502 EII-----GESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEF 556
Query: 555 NKLAQAMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYF 614
KLAQ++ G + G +P +L FL D PD G++ D+ +
Sbjct: 557 GKLAQSIVDGTTVPA-GPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL----NY 611
Query: 615 TKGDKPSATFWSANPRYDLLTEGTYAVVERLQG-ERWISVQDDDDLSLFFRWKVDNTSFH 673
+ + S F+ NPR D + E ++ V+ L G ++W +V DD D F+WK+ + F
Sbjct: 612 SVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF- 670
Query: 674 GFAAIEWEIPTDAISGVYRLKHFGASKKT-IVSPINYFTGASSAFAVQ 720
IEW I D G YR+ H G +KK S + + G S F VQ
Sbjct: 671 SLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVVQ 718
>ref|NP_973628.1| ceramidase family protein [Arabidopsis thaliana]
Length = 792
Score = 541 bits (1394), Expect = e-152
Identities = 274/477 (57%), Positives = 343/477 (71%), Gaps = 10/477 (2%)
Query: 249 LVQIAQLIKATGGKDCNEKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGK 308
L+ IA K++ G ++ ++VR FV AFCQSN GDVSPN LG FCID+G
Sbjct: 319 LLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGL 378
Query: 309 PCDFNHSSCNGNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRH 368
PCDFNHS+CNG + LC GRGPGYP+E ST+IIGE+QF+ AVELF A+E+L GKI Y+H
Sbjct: 379 PCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQH 438
Query: 369 VYLNFTNIEVELDN----KKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNV 424
YL+F+N++V + + VKTCPAA+G GFAAGTTDGPG F F+QGD + + FW+ V
Sbjct: 439 AYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLV 498
Query: 425 RDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTM 484
R+ L+ P QV CQ PKP+LL +GEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTM
Sbjct: 499 RNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTM 558
Query: 485 AGRRLREAVKETLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGP 544
AGRRLR+A+K LIS+ EF+N HVVIAGLTNTYSQYIATFEEY QRYE ASTLYG
Sbjct: 559 AGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGR 618
Query: 545 HTLSAYIQEFNKLAQAMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKE 604
HTL+AYIQEF KLA A+ G + G PPDLL Q S L D+TP G+K GD+K
Sbjct: 619 HTLTAYIQEFKKLATALVNGLTL-PRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKA 677
Query: 605 DIAFPGSGYFTKGDKPSATFWSANPRYDLLTEGTYAVVERL-QGERWISVQDDDDLSLFF 663
D+ P F +G + +ATFWS PR DL+TEG++AVVE L +G +W V DDDD SL F
Sbjct: 678 DV--PPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKF 735
Query: 664 RW-KVDNTSFHGFAAIEWEIPTDAISGVYRLKHFGASKKTIVSPINYFTGASSAFAV 719
+W + S A IEW +P A++GVYR++H+GAS K++ I+ F+G+SSAF V
Sbjct: 736 KWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGAS-KSLFGSISSFSGSSSAFVV 791
Score = 323 bits (828), Expect = 1e-86
Identities = 162/226 (71%), Positives = 188/226 (82%), Gaps = 2/226 (0%)
Query: 19 MQSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFV 78
+ T YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIVAE QG R V
Sbjct: 19 LSRTVYAYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVV 77
Query: 79 FVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLG 138
FVNLDA MASQ++TIK+LERLK+R+G LYTE+NVAISGIHTHAGPGGYLQYV Y VTSLG
Sbjct: 78 FVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLG 137
Query: 139 FVTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYP 198
FV QSFD + N +EQSI+QAH +L+PGS F+N GD+ +A +NRSPS+YL NPA ERS+Y
Sbjct: 138 FVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYK 197
Query: 199 SNVDTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNK 244
+VD +MTL+KFVDS G + GSF+WFATHGTSMS N LISGDNK
Sbjct: 198 YDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNK 242
>dbj|BAD69590.1| neutral ceramidase [Danio rerio] gi|56090224|ref|NP_001007764.1|
N-acylsphingosine amidohydrolase 2 [Danio rerio]
Length = 743
Score = 527 bits (1358), Expect = e-148
Identities = 301/704 (42%), Positives = 408/704 (57%), Gaps = 38/704 (5%)
Query: 26 YLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAG 85
YLIGVG D TGP AD+ MMGYAN +Q G+H RL +R FIV + R VFV D G
Sbjct: 61 YLIGVGRADCTGPVADLPMMGYANTDQTARGLHTRLFSRAFIVDDG--NKRVVFVTSDIG 118
Query: 86 MASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQSFD 145
M SQ L +++ + LK ++G+LY ++NV +SG HTH+G GGY QY ++ +TS G++ S
Sbjct: 119 MVSQRLRLEVFQALKEKYGDLYRQDNVVLSGTHTHSGVGGYFQYTLFMITSKGYIKPSIQ 178
Query: 146 AIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQM 205
AI + + +SI AH NL+PG IFIN G V +++ NRSP +Y+ NP EER+RY N D Q+
Sbjct: 179 AIVSGIVKSIDIAHRNLRPGRIFINKGQVADSNFNRSPHSYMNNPEEERNRYEFNTDKQI 238
Query: 206 TLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGKDCN 265
+LKF D G G SWFA H SM+ N+++S DN +G I + K G
Sbjct: 239 VVLKFTD-LDGDGIGLLSWFAVHPVSMNYTNRMVSSDN--LGYASYIFEQEKNIG----- 290
Query: 266 EKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCN-GNDLLC 324
+ FV F SN+GD SPN+ G C+++G CD+ +SSC G C
Sbjct: 291 --------FLPGEKGPFVAGFSSSNLGDSSPNIRGPVCVNTGLKCDYINSSCPVGGKKAC 342
Query: 325 VGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNKK 384
+ GPG + ST+IIGE F+ A EL+GSA +EL G + H ++N T+ V+ ++
Sbjct: 343 IAFGPG-EDMFESTRIIGENMFKIAKELYGSAKQELHGPVYGAHQWVNMTDETVQFNSTH 401
Query: 385 VVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQVDCQNPKP 443
+TC ALG FAAGTTDG G F F QGD E PFW +RD L PS C PKP
Sbjct: 402 TGRTCKPALGHSFAAGTTDGGGEFNFLQGDTEGDPFWDGIRDAVLGPPSNETKACHQPKP 461
Query: 444 VLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNG 503
+L S+GEM P PW PAI+ +QI+ +G L +++VPGEFTTM+GRR+REAVK L
Sbjct: 462 ILFSTGEMDSPLPWHPAIVDVQIITIGSLAVVAVPGEFTTMSGRRIREAVKREL--EVKE 519
Query: 504 EFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAK 563
F N VV+AGL N Y+ YI T+EEY QRYE AST++GPHTLSAYIQ + LA+A+A
Sbjct: 520 PFTN-AEVVVAGLCNIYTHYITTYEEYQIQRYEGASTIFGPHTLSAYIQRYRGLAKAIAH 578
Query: 564 GD-KIYGNGTSPPDLLSVQKSF--LLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDKP 620
G G PP K F + DP D P G GD+ +++ + + G+
Sbjct: 579 GTIGELPKGPEPP-FFDEDKLFNQVRDPVADVAPVGTTFGDVLQEV----NPVYKVGEIA 633
Query: 621 SATFWSANPRYD-LLTEGTYAVVERLQGE--RWISVQDDDDLSLFFRWKVDNTSFHGFAA 677
S TF S NPR+ + + T VER + W + +D F W + + A
Sbjct: 634 SVTFVSGNPRHSGDIRDTTLVTVERFHNDTGSWEIIHNDASWETRFHW-IKGLAGRSQAK 692
Query: 678 IEWEIPTDAISGVYRLKHFGASKKTI--VSPINYFTGASSAFAV 719
+EW IP A +G Y++++FG K+T + I + G S+AF V
Sbjct: 693 VEWHIPQTAQAGTYQIQYFGHYKQTTENTTVITPYVGTSAAFKV 736
>gb|AAQ04667.1| neutral/alkaline ceramidase [Homo sapiens]
Length = 779
Score = 516 bits (1329), Expect = e-144
Identities = 306/713 (42%), Positives = 411/713 (56%), Gaps = 50/713 (7%)
Query: 23 YGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNL 82
+ Y IGVG D TG AD+N+MGY QN GI RL +R FI+AE R VFV++
Sbjct: 98 FSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSI 157
Query: 83 DAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQ 142
D GM SQ L +++L RL+S++G+LY +NV +SG HTH+GP GY QY V+ + S GF Q
Sbjct: 158 DIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQ 217
Query: 143 SFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVD 202
+F + + +SI AH N+KPG IFIN G+V INRSP +YL NP ER+RYPSN D
Sbjct: 218 TFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYPSNTD 277
Query: 203 TQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGK 262
+M +LK VD +G G SWFA H SM+N+N L++ DN +G
Sbjct: 278 KEMIVLKMVD-LNGDDLGLISWFAIHPVSMNNSNHLVNSDN--VG--------------- 319
Query: 263 DCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSC 317
+S + KN G L FV AF SN+GDVSPN+LG CI++G+ CD +S+C
Sbjct: 320 ----YASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTC 375
Query: 318 N-GNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNI 376
G +C+ +GPG + ST+IIG ++ A EL+ SAS+E+TG + H +++ T++
Sbjct: 376 PIGGPSMCIAKGPG-QDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDV 434
Query: 377 EVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQ 435
V L++ KTC ALG FAAGT DG G F QG E PFW +RD L +PS+
Sbjct: 435 TVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEI 494
Query: 436 VDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 495
+C PKP+LL +GE+ P+PW P I+ +QI+ LG L I ++PGEFTTM+GRRLREAV+
Sbjct: 495 KECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQA 554
Query: 496 TLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFN 555
S+ N T VVI+GL N Y+ YI T+EEY QRYEAAST+YGPH LSAYIQ F
Sbjct: 555 EFASHG---MQNMT-VVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHALSAYIQLFR 610
Query: 556 KLAQAMAKGDKI--YGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
LA+A+A D + G PP + L+ D P G GD+ + P
Sbjct: 611 NLAKAIAT-DTVANLSRGPEPPFFKQLIVP-LIPSIVDRAPKGRTFGDVLQ----PAKPE 664
Query: 614 FTKGDKPSATFWSANPRYDL--LTEGTYAVVERLQ--GERWISVQDDDDLSLFFRWKVDN 669
+ G+ F ANP+ + T T+ VE+ + W V +D F W
Sbjct: 665 YRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGL 724
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASKK-TIVSP--INYFTGASSAFAV 719
A +EW IP A G+YR+++FG ++K I+ P I F G S AF V
Sbjct: 725 LGLSN-ATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEV 776
>gb|AAL06061.1| mitochondrial ceramidase [Homo sapiens] gi|9845267|ref|NP_063946.1|
N-acylsphingosine amidohydrolase 2 [Homo sapiens]
gi|9246993|gb|AAF86240.1| mitochondrial ceramidase [Homo
sapiens]
Length = 761
Score = 516 bits (1329), Expect = e-144
Identities = 306/713 (42%), Positives = 411/713 (56%), Gaps = 50/713 (7%)
Query: 23 YGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNL 82
+ Y IGVG D TG AD+N+MGY QN GI RL +R FI+AE R VFV++
Sbjct: 80 FSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSI 139
Query: 83 DAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQ 142
D GM SQ L +++L RL+S++G+LY +NV +SG HTH+GP GY QY V+ + S GF Q
Sbjct: 140 DIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQ 199
Query: 143 SFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVD 202
+F + + +SI AH N+KPG IFIN G+V INRSP +YL NP ER+RYPSN D
Sbjct: 200 TFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYPSNTD 259
Query: 203 TQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGK 262
+M +LK VD +G G SWFA H SM+N+N L++ DN +G
Sbjct: 260 KEMIVLKMVD-LNGDDLGLISWFAIHPVSMNNSNHLVNSDN--VG--------------- 301
Query: 263 DCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSC 317
+S + KN G L FV AF SN+GDVSPN+LG CI++G+ CD +S+C
Sbjct: 302 ----YASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTC 357
Query: 318 N-GNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNI 376
G +C+ +GPG + ST+IIG ++ A EL+ SAS+E+TG + H +++ T++
Sbjct: 358 PIGGPSMCIAKGPG-QDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDV 416
Query: 377 EVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQ 435
V L++ KTC ALG FAAGT DG G F QG E PFW +RD L +PS+
Sbjct: 417 TVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEI 476
Query: 436 VDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 495
+C PKP+LL +GE+ P+PW P I+ +QI+ LG L I ++PGEFTTM+GRRLREAV+
Sbjct: 477 KECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQA 536
Query: 496 TLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFN 555
S+ N T VVI+GL N Y+ YI T+EEY QRYEAAST+YGPH LSAYIQ F
Sbjct: 537 EFASHG---MQNMT-VVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHALSAYIQLFR 592
Query: 556 KLAQAMAKGDKI--YGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
LA+A+A D + G PP + L+ D P G GD+ + P
Sbjct: 593 NLAKAIAT-DTVANLSRGPEPPFFKQLIVP-LIPSIVDRAPKGRTFGDVLQ----PAKPE 646
Query: 614 FTKGDKPSATFWSANPRYDL--LTEGTYAVVERLQ--GERWISVQDDDDLSLFFRWKVDN 669
+ G+ F ANP+ + T T+ VE+ + W V +D F W
Sbjct: 647 YRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGL 706
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASKK-TIVSP--INYFTGASSAFAV 719
A +EW IP A G+YR+++FG ++K I+ P I F G S AF V
Sbjct: 707 LGLSN-ATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEV 758
>dbj|BAC92751.1| ceramidase [Dictyostelium discoideum] gi|66800203|ref|XP_629027.1|
hypothetical protein DDB0215370 [Dictyostelium
discoideum] gi|60462360|gb|EAL60581.1| hypothetical
protein DDB0215370 [Dictyostelium discoideum]
Length = 714
Score = 516 bits (1328), Expect = e-144
Identities = 290/703 (41%), Positives = 414/703 (58%), Gaps = 36/703 (5%)
Query: 26 YLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAG 85
Y IG G YD+TGP A+ NMMGYA Q T GIHFR RAR F+ ++ +G R V+V+ D+
Sbjct: 40 YQIGTGIYDITGPGAETNMMGYAMPGQITGGIHFRQRARAFVFIDS-EGNRAVYVSTDSC 98
Query: 86 MASQLLTIKLLERLKSRFG-NLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQSF 144
M Q + I++++ L+ FG LYT +NV +SG HTH+GP G+ +Y +Y +T+LGF ++F
Sbjct: 99 MIFQEVKIQVIQDLQEIFGPTLYTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNF 158
Query: 145 DAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQ 204
D I + + Q+I++AH +++P + G++ ++INRSP AY NP EE++ Y +NVD
Sbjct: 159 DTICDGIVQAIVKAHKSVQPARMLTQQGELWNSNINRSPYAYDNNPEEEKAMYDANVDKN 218
Query: 205 MTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGKDC 264
MT+++ ++ SG + S+F H TSM+N N LISGDNK +
Sbjct: 219 MTVIR-IEDMSGNPFAAISFFGVHCTSMNNTNHLISGDNKGYASYLW------------- 264
Query: 265 NEKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLC 324
EK + G F+ AF QSN GDVSPN G C D GKPCD+ S+CNG C
Sbjct: 265 -EKHANGQSSLPGTGP-FIAAFGQSNEGDVSPNTRGPTCRD-GKPCDYKTSTCNGKVEEC 321
Query: 325 VGRGPGYPNEIL-STKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNK 383
GPG ++ ST+IIG QF A+ELF +A+ +++GKI YRH + FTN+ VE
Sbjct: 322 WALGPGTDGDMFESTQIIGGNQFNKALELFNNATIQVSGKIQYRHTWKPFTNVSVEAPYN 381
Query: 384 KVVK---TCPAALGPGFAAGTTDGPGVFGFQQGD-PEISPFWKNVRDFLKEPSQYQVDCQ 439
V+ TC A+G FA GTTDGPG F F QGD +PFW + + +P+ Q CQ
Sbjct: 382 SGVEGATTCRGAMGYSFAGGTTDGPGAFNFIQGDNSTTNPFWNFIGGIIAKPTPQQTACQ 441
Query: 440 NPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS 499
PKP+L+ G M +P PW P ++P+QI+ LG++++++VPGEFTTM+GRRLR V+E +
Sbjct: 442 APKPILIDVG-MVEPIPWVPDVMPLQIITLGQIVLVAVPGEFTTMSGRRLRNTVREII-- 498
Query: 500 NSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQ 559
G+ V+IAGL NTYS YIATFEE+ QRYE AST++GPHTL AY QEF LAQ
Sbjct: 499 ---GQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQEFANLAQ 555
Query: 560 AMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDK 619
++ G + GT P ++ FL D P GDI D++ + ++
Sbjct: 556 SIVDGSQA-DPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVS--TTTPYSINQT 612
Query: 620 PSATFWSANPRYDLLTEGTYAVVERLQGE-RWISVQDDDDLSLFFRWKVDNTSFHGFAAI 678
+ F+ AN R + +TE ++ V++LQ +W ++ +D D WK+ + F +
Sbjct: 613 VTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGF-SLITV 671
Query: 679 EWEIPTDAISGVYRLKHFGASKKTIVSP-INYFTGASSAFAVQ 720
+W I G YR+ H G +KK S + ++ G SS F VQ
Sbjct: 672 DWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGISSNFNVQ 714
>gb|AAB69633.1| random slug cDNA25 protein [Dictyostelium discoideum]
Length = 702
Score = 516 bits (1328), Expect = e-144
Identities = 290/703 (41%), Positives = 414/703 (58%), Gaps = 36/703 (5%)
Query: 26 YLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAG 85
Y IG G YD+TGP A+ NMMGYA Q T GIHFR RAR F+ ++ +G R V+V+ D+
Sbjct: 28 YQIGTGIYDITGPGAETNMMGYAMPGQITGGIHFRQRARAFVFIDS-EGNRAVYVSTDSC 86
Query: 86 MASQLLTIKLLERLKSRFG-NLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQSF 144
M Q + I++++ L+ FG LYT +NV +SG HTH+GP G+ +Y +Y +T+LGF ++F
Sbjct: 87 MIFQEVKIQVIQDLQEIFGPTLYTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNF 146
Query: 145 DAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQ 204
D I + + Q+I++AH +++P + G++ ++INRSP AY NP EE++ Y +NVD
Sbjct: 147 DTICDGIVQAIVKAHKSVQPARMLTQQGELWNSNINRSPYAYDNNPEEEKAMYDANVDKN 206
Query: 205 MTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGKDC 264
MT+++ ++ SG + S+F H TSM+N N LISGDNK +
Sbjct: 207 MTVIR-IEDMSGNPFAAISFFGVHCTSMNNTNHLISGDNKGYASYLW------------- 252
Query: 265 NEKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLC 324
EK + G F+ AF QSN GDVSPN G C D GKPCD+ S+CNG C
Sbjct: 253 -EKHANGQSSLPGTGP-FIAAFGQSNEGDVSPNTRGPTCRD-GKPCDYKTSTCNGKVEEC 309
Query: 325 VGRGPGYPNEIL-STKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNK 383
GPG ++ ST+IIG QF A+ELF +A+ +++GKI YRH + FTN+ VE
Sbjct: 310 WALGPGTDGDMFESTQIIGGNQFNKALELFNNATIQVSGKIQYRHTWKPFTNVSVEAPYN 369
Query: 384 KVVK---TCPAALGPGFAAGTTDGPGVFGFQQGD-PEISPFWKNVRDFLKEPSQYQVDCQ 439
V+ TC A+G FA GTTDGPG F F QGD +PFW + + +P+ Q CQ
Sbjct: 370 SGVEGATTCRGAMGYSFAGGTTDGPGAFNFIQGDNSTTNPFWNFIGGIIAKPTPQQTACQ 429
Query: 440 NPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS 499
PKP+L+ G M +P PW P ++P+QI+ LG++++++VPGEFTTM+GRRLR V+E +
Sbjct: 430 APKPILIDVG-MVEPIPWVPDVMPLQIITLGQIVLVAVPGEFTTMSGRRLRNTVREII-- 486
Query: 500 NSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQ 559
G+ V+IAGL NTYS YIATFEE+ QRYE AST++GPHTL AY QEF LAQ
Sbjct: 487 ---GQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQEFANLAQ 543
Query: 560 AMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDK 619
++ G + GT P ++ FL D P GDI D++ + ++
Sbjct: 544 SIVDGSQA-DPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVS--TTTPYSINQT 600
Query: 620 PSATFWSANPRYDLLTEGTYAVVERLQGE-RWISVQDDDDLSLFFRWKVDNTSFHGFAAI 678
+ F+ AN R + +TE ++ V++LQ +W ++ +D D WK+ + F +
Sbjct: 601 VTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGF-SLITV 659
Query: 679 EWEIPTDAISGVYRLKHFGASKKTIVSP-INYFTGASSAFAVQ 720
+W I G YR+ H G +KK S + ++ G SS F VQ
Sbjct: 660 DWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGISSNFNVQ 702
>emb|CAI15870.1| N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [Homo
sapiens]
Length = 761
Score = 515 bits (1326), Expect = e-144
Identities = 306/713 (42%), Positives = 411/713 (56%), Gaps = 50/713 (7%)
Query: 23 YGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNL 82
+ Y IGVG D TG AD+N+MGY QN GI RL +R FI+AE R VFV++
Sbjct: 80 FSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSI 139
Query: 83 DAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQ 142
D GM SQ L +++L RL+S++G+LY +NV +SG HTH+GP GY QY V+ + S GF Q
Sbjct: 140 DIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQ 199
Query: 143 SFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVD 202
+F + + +SI AH N+KPG IFIN G+V INRSP +YL NP ER+RY SN D
Sbjct: 200 TFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTD 259
Query: 203 TQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGK 262
+M +LK VD +G G SWFA H SM+N+N L++ DN +G
Sbjct: 260 KEMIVLKMVD-LNGDDLGLISWFAIHPVSMNNSNHLVNSDN--VG--------------- 301
Query: 263 DCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSC 317
+S + KN G L FV AF SN+GDVSPN+LG CI++G+ CD +S+C
Sbjct: 302 ----YASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTC 357
Query: 318 N-GNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNI 376
G +C+ +GPG + ST+IIG ++ A EL+ SAS+E+TG + H +++ T++
Sbjct: 358 PIGGPSMCIAKGPG-QDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDV 416
Query: 377 EVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQ 435
V L++ KTC ALG FAAGT DG G F QG E PFW +RD L +PS+
Sbjct: 417 TVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEI 476
Query: 436 VDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 495
+C PKP+LL +GE+ P+PW P I+ +QI+ LG L I ++PGEFTTM+GRRLREAV+
Sbjct: 477 KECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQA 536
Query: 496 TLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFN 555
S+ N T VVI+GL N Y+ YI T+EEY QRYEAAST+YGPHTLSAYIQ F
Sbjct: 537 EFASHG---MQNMT-VVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFR 592
Query: 556 KLAQAMAKGDKI--YGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
LA+A+A D + G PP + L+ D P G GD+ + P
Sbjct: 593 NLAKAIAT-DTVANLSRGPEPPFFKQLIVP-LIPSIVDRAPKGRTFGDVLQ----PAKPE 646
Query: 614 FTKGDKPSATFWSANPRYDL--LTEGTYAVVERLQ--GERWISVQDDDDLSLFFRWKVDN 669
+ G+ F ANP+ + T T+ VE+ + W V +D F W
Sbjct: 647 YRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGL 706
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASKK-TIVSP--INYFTGASSAFAV 719
A +EW IP A G+YR+++FG ++K I+ P I F G S AF V
Sbjct: 707 LGLSN-ATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEV 758
>ref|XP_309165.2| ENSANGP00000018598 [Anopheles gambiae str. PEST]
gi|55245040|gb|EAA04917.2| ENSANGP00000018598 [Anopheles
gambiae str. PEST]
Length = 696
Score = 514 bits (1324), Expect = e-144
Identities = 294/710 (41%), Positives = 422/710 (59%), Gaps = 40/710 (5%)
Query: 22 TYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVN 81
T G Y +GVG D TGP+ ++ MGYA + Q GIH R AR++++ E+ G R VFV+
Sbjct: 16 TTGAYRVGVGRADCTGPSVEITFMGYAQVTQRGTGIHLRQYARSYVI-EDENGTRVVFVS 74
Query: 82 LDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVT 141
+DAGM + +L L+ ++G LYT NV ISG HTH+ PGG+L Y++Y +TSLGFV
Sbjct: 75 VDAGMMGHAVKRDVLAVLQKKYGELYTHANVVISGTHTHSTPGGFLMYLLYDLTSLGFVP 134
Query: 142 QSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNV 201
++F+A+ + + QS+I+AHNNL +++ +V EA+INRSPSAY NP EER++Y NV
Sbjct: 135 ETFNALVHGIAQSVIRAHNNLVEARLYVAETEVLEANINRSPSAYENNPEEERAQYRHNV 194
Query: 202 DTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGG 261
D + L+FV + +G G+ +WFA H TSM+N N+L+S DN + ++
Sbjct: 195 DKTLVQLQFVHARTGAPFGAINWFAVHPTSMNNTNRLVSSDNVGLAAIL----------- 243
Query: 262 KDCNEKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGND 321
++ + + V K D FVGAF SN+GDVSPN+ G C +G PCD SSC
Sbjct: 244 --LEQERNPDALVGKGD---FVGAFASSNLGDVSPNINGPKCEKTGLPCDVLTSSCPSGA 298
Query: 322 LLCVGRGPGYPNEILSTKIIGERQFRSAVELFG---SASEELTGKIDYRHVYLNFTNIE- 377
C GPG + ST+II +R +R+A L + + E+TG + + H Y++ T E
Sbjct: 299 GACFASGPG-RDMFESTEIIADRLYRAAAGLLSTRTTKAREVTGPVAFVHQYVDMTTAET 357
Query: 378 VELD---NKKV-VKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQ 433
V D K V V+ C A+G FAAGTTDGPG F FQQG + W VRDF+ EP+Q
Sbjct: 358 VHFDRATQKPVRVRGCLPAMGYSFAAGTTDGPGAFDFQQGTITDNVLWNAVRDFIAEPTQ 417
Query: 434 YQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAV 493
DCQ PKP+LL++G PY W P ++P Q+L +G + +VPGEFTTMAGRRLR AV
Sbjct: 418 DDKDCQAPKPILLATGRARFPYDWQPKVVPTQLLVVGDFAMAAVPGEFTTMAGRRLRSAV 477
Query: 494 KETLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQE 553
++ + VV+AGL+N YS YI T EEY QRYE ASTLYGPHTL+ Y+++
Sbjct: 478 ARASVAAG----GRDLQVVVAGLSNMYSSYITTPEEYEIQRYEGASTLYGPHTLTLYLEQ 533
Query: 554 FNKLAQAMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
+ +LA A+ + + + G +PP Q S D+ P G + GD+K P + Y
Sbjct: 534 YKRLAGALVRNETVPA-GPTPPYEDDKQISLTTGVIFDSHPLGKEFGDVK---VHPEAAY 589
Query: 614 FTKGDKPSATFWSANPRYDLLTEGTYAVVERLQGE-RWISVQDDDDLSLFFRWKVDNTSF 672
+G SA F + NPR +L+ + ++ VE+ Q + W V D + FRW+ +T
Sbjct: 590 -ERGATVSAVFIAGNPRNNLMHDKSFFTVEQQQPDGNWSVVATDANWETRFRWERTSTIL 648
Query: 673 HGFAAIE--WEIPTDAISGVYRLKHFGASKKTIVSPINYFTGASSAFAVQ 720
GF+ IE W I + + G+YR++HFG + I+ + + GA++AF+V+
Sbjct: 649 -GFSDIEFSWTIGPNTLDGLYRIRHFG-YYRYILGGVYPYVGATNAFSVK 696
>ref|NP_061300.1| N-acylsphingosine amidohydrolase 2 [Mus musculus]
gi|7576311|dbj|BAA94546.1| nerutal ceramidase [Mus
musculus] gi|7576309|dbj|BAA94545.1| neutral ceramidase
[Mus musculus]
Length = 756
Score = 504 bits (1299), Expect = e-141
Identities = 295/713 (41%), Positives = 409/713 (56%), Gaps = 50/713 (7%)
Query: 23 YGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNL 82
+ Y IGVG D TG +D+N+MGY QN G+ RL +R FI+A+ R FV++
Sbjct: 75 FSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSV 134
Query: 83 DAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQ 142
+ M SQ L +++L+RL+S++G+LY +NV +S IHTH+GP G+ QY +Y + S GF +
Sbjct: 135 ELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNR 194
Query: 143 SFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVD 202
+F I + + +SI AH NLKPG IFIN G+V INRSPS+YLLNP ER+RY SN D
Sbjct: 195 TFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTD 254
Query: 203 TQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGK 262
+M +LK VD +G+ G SWFA H SM+N+N ++ DN
Sbjct: 255 KEMLVLKLVD-LNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGY---------------- 297
Query: 263 DCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSC 317
++ + KN G L FV F SN+GDVSPN+LG C+++G+ CD + S+C
Sbjct: 298 -----AAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTC 352
Query: 318 -NGNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNI 376
NG +C+ GPG + ST IIG ++ A EL+ SAS+E+TG + H ++N T++
Sbjct: 353 PNGGPSMCMASGPG-QDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDV 411
Query: 377 EVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQ 435
V+L+ VKTC ALG FAAGT DG QG E PFW +RD L +PS+
Sbjct: 412 SVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEI 471
Query: 436 VDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 495
V+CQ PKP+LL SGE+ P+PW P I+ +QI+ +G L I ++PGE TTM+GRR REA+K+
Sbjct: 472 VECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKK 531
Query: 496 TLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFN 555
+ VVIAGL+N Y+ YI T+EEY QRYEAAST+YGPHTLSAYIQ F
Sbjct: 532 EFALYGMKDMT----VVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFR 587
Query: 556 KLAQAMAKGDKI--YGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
LA+A+A D + +G PP ++ S L+ D P G GD+ + P
Sbjct: 588 DLAKAIAT-DTVANMSSGPEPPFFKNLIAS-LIPNIADRAPIGKHFGDVLQ----PAKPE 641
Query: 614 FTKGDKPSATFWSANPR--YDLLTEGTYAVVERLQGE--RWISVQDDDDLSLFFRWKVDN 669
+ G+ F ANP+ + T T+ VE+ + W + +D F W
Sbjct: 642 YRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGI 701
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASKK-TIVSP--INYFTGASSAFAV 719
A I W IP A G+YR+++FG ++K ++ P I F G SS F V
Sbjct: 702 LGLSN-ATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEV 753
>emb|CAF92687.1| unnamed protein product [Tetraodon nigroviridis]
Length = 746
Score = 504 bits (1299), Expect = e-141
Identities = 284/712 (39%), Positives = 409/712 (56%), Gaps = 48/712 (6%)
Query: 20 QSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVF 79
++ + YLIGVG D TGP A++ ++ + Q +H RL +R FI+ ++ Q R VF
Sbjct: 70 ETEHKPYLIGVGRADCTGPPAEIPLVSFEPGLQAQVTVHTRLYSRAFIIDDSQQ--RVVF 127
Query: 80 VNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGF 139
V D GM SQ L +++L+ LK ++GNLY ++NV +SG HTH GP GY QY ++ +TS G+
Sbjct: 128 VTADVGMVSQRLRLEVLKALKEKYGNLYRQDNVVLSGTHTHCGPAGYFQYTLFMITSKGY 187
Query: 140 VTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPS 199
+ +S + N + +SI AH+N+KPG I+ + GD+ +SINRSP +Y+ NP EER+RY
Sbjct: 188 IKESIQPLVNGIVKSIDLAHSNMKPGRIYRSRGDLDGSSINRSPHSYMNNPEEERNRYKW 247
Query: 200 NVDTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKAT 259
N D Q+ +LKF D G G SWFA H SM+ N+++S DN
Sbjct: 248 NTDKQVLVLKFTD-LDGDGIGMLSWFAVHAVSMNYTNRMVSSDNMGY------------- 293
Query: 260 GGKDCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNH 314
+S + KN G L FV F SN+GDV+PN G C++SG PCD+ +
Sbjct: 294 --------ASYLMEQDKNPGELPGKGGFVAGFSSSNLGDVTPNTKGPHCVNSGLPCDYLN 345
Query: 315 SSCN-GNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNF 373
SSC G +C GPG + ST+IIG + A EL+ +A EE+TG + H +++
Sbjct: 346 SSCPVGGTKMCQSSGPG-ADMFESTRIIGHNIYMKAKELYANAKEEVTGFLQSAHQWVDM 404
Query: 374 TNIEVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPS 432
+N+ +++++ V+TC ALG FAAGTTDG G F QG PFW +RD + +PS
Sbjct: 405 SNVTIQINSTHTVRTCKPALGHSFAAGTTDGGGDLNFTQGSVVGDPFWDGIRDAIVGKPS 464
Query: 433 QYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREA 492
+C PKP+L S+GEM P PW P I+ +QI+ +G + +++VPGE TTM+GRRLRE+
Sbjct: 465 NETQECHQPKPILFSTGEMSKPLPWHPNIIDVQIITIGSVAVVAVPGEMTTMSGRRLRES 524
Query: 493 VKETLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQ 552
VK+ L S G F + VVIAGL+N Y+ YI TFEEY QRYE AST++GP+TLSAY+Q
Sbjct: 525 VKQEL--QSEGAF-RDVEVVIAGLSNVYTHYITTFEEYQVQRYEGASTIFGPYTLSAYLQ 581
Query: 553 EFNKLAQAMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSG 612
++ LA+A+A+ + G PP + F D P+ GD+ + +P
Sbjct: 582 KYRGLARAIAQAE--LPTGPQPPFFEKLFNLMFAAGF-DRKPENRSFGDVIGQV-YP--- 634
Query: 613 YFTKGDKPSATFWSANPRYD-LLTEGTYAVVERLQG--ERWISVQDDDDLSLFFRWKVDN 669
+ +GD S TF + NPR+ + + T+ VE + W +V D F W +
Sbjct: 635 VYRQGDVVSVTFVAGNPRHSGDMRDKTFVAVEIYDNRTDTWNTVHTDASWETRFHW-LKG 693
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASK--KTIVSPINYFTGASSAFAV 719
++ + +EW IP A SG YR+KHFG K K + I + G S F V
Sbjct: 694 SNRQSNSTVEWYIPPSAASGFYRIKHFGHYKQMKGLKPVITPYEGTSDVFRV 745
>dbj|BAC38089.1| unnamed protein product [Mus musculus]
Length = 756
Score = 504 bits (1298), Expect = e-141
Identities = 294/713 (41%), Positives = 409/713 (57%), Gaps = 50/713 (7%)
Query: 23 YGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNL 82
+ Y IGVG D TG +D+N+MGY QN G+ RL +R FI+A+ R FV++
Sbjct: 75 FSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSV 134
Query: 83 DAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQ 142
+ M SQ L +++L+RL+S++G+LY +NV +S IHTH+GP G+ QY +Y + S GF +
Sbjct: 135 ELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNR 194
Query: 143 SFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVD 202
+F I + + +SI AH NLKPG IFIN G+V INRSPS+YLLNP ER+RY SN D
Sbjct: 195 TFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTD 254
Query: 203 TQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGK 262
+M +LK VD +G+ G SWFA H SM+N+N ++ DN
Sbjct: 255 KEMLVLKLVD-LNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGY---------------- 297
Query: 263 DCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSC 317
++ + KN G L FV F SN+GDVSPN+LG C+++G+ CD + S+C
Sbjct: 298 -----AAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTC 352
Query: 318 -NGNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNI 376
NG +C+ GPG + ST IIG ++ A EL+ SAS+E+TG + H ++N T++
Sbjct: 353 PNGGPSMCMASGPG-QDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDV 411
Query: 377 EVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQ 435
V+L+ VKTC ALG FAAGT DG QG E PFW +RD L +PS+
Sbjct: 412 SVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEI 471
Query: 436 VDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 495
V+CQ PKP+LL SGE+ P+PW P I+ +QI+ +G L I ++PGE TTM+GRR REA+K+
Sbjct: 472 VECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKK 531
Query: 496 TLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFN 555
+ +VIAGL+N Y+ YI T+EEY QRYEAAST+YGPHTLSAYIQ F
Sbjct: 532 EFALYGMKDMT----IVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFR 587
Query: 556 KLAQAMAKGDKI--YGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
LA+A+A D + +G PP ++ S L+ D P G GD+ + P
Sbjct: 588 DLAKAIAT-DTVANMSSGPEPPFFKNLIAS-LIPNIADRAPIGKHFGDVLQ----PAKPE 641
Query: 614 FTKGDKPSATFWSANPR--YDLLTEGTYAVVERLQGE--RWISVQDDDDLSLFFRWKVDN 669
+ G+ F ANP+ + T T+ VE+ + W + +D F W
Sbjct: 642 YRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGI 701
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASKK-TIVSP--INYFTGASSAFAV 719
A I W IP A G+YR+++FG ++K ++ P I F G SS F V
Sbjct: 702 LGLSN-ATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEV 753
>ref|NP_446098.1| N-acylsphingosine amidohydrolase 2 [Rattus norvegicus]
gi|14701598|dbj|BAB62033.1| ceramidase [Rattus
norvegicus]
Length = 761
Score = 501 bits (1289), Expect = e-140
Identities = 292/713 (40%), Positives = 411/713 (56%), Gaps = 50/713 (7%)
Query: 23 YGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNL 82
+ Y IGVG D TG +D+N+MGY QN G+ RL +R FI+A+ R FV++
Sbjct: 80 FSGYYIGVGRADCTGQVSDINLMGYGKNGQNAQGLLTRLFSRAFILADPDGSNRMAFVSV 139
Query: 83 DAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQ 142
+ M SQ L +++L+RL+S++G+LY +NV +S HTH+GP G+ QY +Y + S GF +
Sbjct: 140 ELCMISQRLRLEVLKRLQSKYGSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNR 199
Query: 143 SFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVD 202
+F I + + +SI AH NLKPG + IN G+V INRSPS+YL NP ER+RY S+ D
Sbjct: 200 TFQYIVSGIVKSIDIAHTNLKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTD 259
Query: 203 TQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKDIGELVQIAQLIKATGGK 262
+M +LK VD +G+ G SWFA H SM+N+N L++ DN
Sbjct: 260 KEMVVLKLVD-LNGEDLGLISWFAVHPVSMNNSNHLVNSDNMGY---------------- 302
Query: 263 DCNEKSSQASKVRKNDGSL-----FVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSC 317
++ + KN G L FV F SN+GDVSPN+LG C+++G+ CD + S+C
Sbjct: 303 -----AAYLFEQEKNRGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTC 357
Query: 318 -NGNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNI 376
+G +C+ GPG + ST IIG ++ A EL SAS+E+TG + H ++N T++
Sbjct: 358 PSGGPSMCMASGPG-QDMFESTHIIGRVIYQKAKELHASASQEVTGPVLTAHQWVNMTDV 416
Query: 377 EVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRD-FLKEPSQYQ 435
V+L+ VKTC AALG FAAGT DG QG E + FW +RD L +PS+
Sbjct: 417 SVQLNATHTVKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEI 476
Query: 436 VDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 495
++CQ PKP+L+ +GE+ P+PW P I+ IQI+ LG L I ++PGEFTTM+GRRLREAVK+
Sbjct: 477 IECQKPKPILIHTGELTKPHPWQPDIVDIQIVTLGSLAIAAIPGEFTTMSGRRLREAVKK 536
Query: 496 TLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFN 555
+ VVIAGL+N Y+ YI T+EEY QRYEAAST+YGPHTLSAYIQ F
Sbjct: 537 EFALYGMKDMT----VVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFR 592
Query: 556 KLAQAMAKGDKI--YGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGY 613
LA+A+A D + +G PP ++ S L+ D P G + GD+ + P
Sbjct: 593 ALAKAIAT-DTVANMSSGPEPPFFKNLIGS-LIPNIADRAPIGKQFGDVLQ----PAKPE 646
Query: 614 FTKGDKPSATFWSANPR--YDLLTEGTYAVVERLQGE--RWISVQDDDDLSLFFRWKVDN 669
+ G+ F ANP+ + T T+ VE+ + W + +D F W
Sbjct: 647 YRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYEDSVANWQIMHNDASWETRFYWHKGV 706
Query: 670 TSFHGFAAIEWEIPTDAISGVYRLKHFGASKK-TIVSP--INYFTGASSAFAV 719
A I W IP A+ GVYR+++FG ++K ++ P I F G SS F +
Sbjct: 707 LGLSN-ATIHWHIPDTALPGVYRIRYFGHNRKQELLKPAVILAFEGISSPFEI 758
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.317 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,313,864,485
Number of Sequences: 2540612
Number of extensions: 60153726
Number of successful extensions: 121898
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 55
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 121438
Number of HSP's gapped (non-prelim): 85
length of query: 720
length of database: 863,360,394
effective HSP length: 135
effective length of query: 585
effective length of database: 520,377,774
effective search space: 304420997790
effective search space used: 304420997790
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 79 (35.0 bits)
Medicago: description of AC146743.14