BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144728.4 + phase: 0
(566 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultiva... 467 e-130
pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilg... 430 e-119
emb|CAA73733.1| pectin methylesterase-like protein [Zea mays] gi... 429 e-119
gb|AAF26136.1| putative pectinesterase [Arabidopsis thaliana] gi... 416 e-114
dbj|BAC42986.1| putative pectinesterase [Arabidopsis thaliana] g... 412 e-113
gb|AAC34241.1| putative pectinesterase [Arabidopsis thaliana] gi... 408 e-112
gb|AAC27719.1| flower-specific pectin methylesterase precursor [... 405 e-111
ref|NP_198139.1| pectinesterase family protein [Arabidopsis thal... 405 e-111
gb|AAV91509.1| VGD1-like protein 1 [Arabidopsis thaliana] 403 e-111
emb|CAA39658.1| Bp19 [Brassica napus] gi|99823|pir||S14952 pecti... 400 e-110
emb|CAB71877.1| PECTINESTERASE-like protein [Arabidopsis thalian... 399 e-109
dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica ... 399 e-109
gb|AAV91510.1| VGD1-like protein 2 [Arabidopsis thaliana] 394 e-108
ref|XP_479611.1| putative pectin methylesterase [Oryza sativa (j... 390 e-107
gb|AAM65978.1| pectin methylesterase [Arabidopsis thaliana] gi|1... 389 e-106
gb|AAF23892.1| pectin methyl esterase [Solanum tuberosum] 385 e-105
pir||T52325 pectinesterase (EC 3.1.1.11) [imported] - turnip (fr... 383 e-104
gb|AAP40488.1| putative pectin methylesterase [Arabidopsis thali... 382 e-104
gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis thaliana] gi|92795... 382 e-104
emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum] 380 e-104
>emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultivar-group)]
gi|50924778|ref|XP_472740.1| OSJNBa0036B21.20 [Oryza
sativa (japonica cultivar-group)]
Length = 568
Score = 467 bits (1202), Expect = e-130
Identities = 246/574 (42%), Positives = 354/574 (60%), Gaps = 19/574 (3%)
Query: 1 MERKIFVSGVSLILVVGVALGVVALVRTNNGPADAN----NGGELTSHTKAVTAVCQNSD 56
M +K + GV ILVV V +GVVA V + A N L + K+V ++C +
Sbjct: 1 MAQKFLLGGVGAILVVAVVVGVVATVTRSGNKAGDNFTVPGEANLATSGKSVESLCAPTL 60
Query: 57 DHKFCADTLGSVN--TSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALD 114
+ C TL + T +P + V K+++ES+ A + + E + ++ T+ A +
Sbjct: 61 YKESCEKTLTTATSGTENPKEVFSTVAKSALESIKSAVEKSKAIG-EAKTSDSMTESARE 119
Query: 115 DCKDLLEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEK 174
DCK LLE ++D+L+ +A + V + R+ DL++WL V + +C DGF ++
Sbjct: 120 DCKALLEDSVDDLRGMVEMAGGDVKV--LFSRSDDLEHWLTGVMTFMDTCADGF---ADE 174
Query: 175 QVQSQLQTGSLDHVGKLTALALDVVTAITKVLAALDLDL--NVKPSSRRLFEVDED--GN 230
++++ + + L + +L++ AL + + + LDLD+ P R L E G
Sbjct: 175 KLKADMHS-VLRNASELSSNALAITNTLGAIFKKLDLDMFKGENPIHRSLIAEQETVGGF 233
Query: 231 PEWMSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAG 290
P WM DRKLLA PNAVVA+DGSG+FKT+ +A+NS PK HQGRYVIYVKAG
Sbjct: 234 PSWMKAPDRKLLASGDRNRP-QPNAVVAQDGSGQFKTIQEAVNSMPKGHQGRYVIYVKAG 292
Query: 291 VYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFE 350
+YDE + + K K NI +YGDGP ++ +TG+K+F DG+ T++TATFS A GFI K M F
Sbjct: 293 LYDEIVMVPKDKVNIFMYGDGPKRSRVTGRKSFADGITTMKTATFSVEAAGFICKNMGFH 352
Query: 351 NTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYAS 410
NTAGA +HQAVALR+ GD AF++C +QDTLY HA RQF+RNC ISGT+DFIFG ++
Sbjct: 353 NTAGAERHQAVALRINGDLGAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSA 412
Query: 411 TVIQNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLA 470
V QN I+ R+P NQQN + A G NM +G+V+QNC ++P+ L PDR K+ S+L
Sbjct: 413 AVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLG 472
Query: 471 RPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKW-GK 529
RPWK YSR + ME+TI D I+P+G++PW G L+T ++AE+ N GPG+ RV W G
Sbjct: 473 RPWKEYSRLVIMESTIADFIKPEGYMPWNGEFALNTLYYAEFNNRGPGAGTSKRVNWKGF 532
Query: 530 GVLSKADATKYTAAQWIEGGVWLPATGIPFDLGF 563
V+ + +A ++TA +++GG WL TG P LGF
Sbjct: 533 RVIGQKEAEQFTAGPFVDGGTWLKFTGTPHFLGF 566
>pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilgiana
gi|6714532|dbj|BAA89480.1| pectin methylesterase [Salix
gilgiana]
Length = 596
Score = 430 bits (1105), Expect = e-119
Identities = 237/587 (40%), Positives = 342/587 (57%), Gaps = 37/587 (6%)
Query: 1 MERKIFVSGVSLI-LVVGVALGVVALVRTNNGPADANNGGELTSHTK------------- 46
+ +KI ++ VS I V V GV +LV ++ + NNGG ++ T+
Sbjct: 21 LRKKIIIASVSSIAFFVIVGAGVFSLVSNHDISSPGNNGGSPSAATRPAEKAKPISHVAR 80
Query: 47 AVTAVCQNSDDHKFCADTLGS------VNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAV 100
+ VC + C +TL ++ P D +K +K + E + K
Sbjct: 81 VIKTVCNATTYQDTCQNTLEKGVLGKDPSSVQPKDLLKIAIKAADEEIDKVIKKASSFKF 140
Query: 101 ENEKNNQSTKMALDDCKDLLEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAY 160
+ + K A DDC +L+E A +EL+ S + + + + A DL NWL AV +Y
Sbjct: 141 DKPRE----KAAFDDCLELIEDAKEELKNS--VDCIGNDIGKLASNAPDLSNWLSAVMSY 194
Query: 161 QQSCLDGFDTDGEKQVQSQLQTGSLDHVGKLTALALDVVTAITKVLAALD----LDLNVK 216
QQ+C+DGF E +++S ++ + +LT+ +L +V+++ L L+ +
Sbjct: 195 QQTCIDGFP---EGKLKSDMEK-TFKATRELTSNSLAMVSSLVSFLKNFSFSGTLNRRLL 250
Query: 217 PSSRRLFEVDEDGNPEWMSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYP 276
+ +D+DG P WMS DR++L PN VAKDGSG FKT+ +A+ + P
Sbjct: 251 AEEQNSPSLDKDGVPGWMSHEDRRILKGADKDKP-KPNVSVAKDGSGDFKTISEALAAMP 309
Query: 277 KNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFS 336
++GRYVI+VK GVYDE + + K NI +YGDG KTI+TG KNF DGV+T +TATF+
Sbjct: 310 AKYEGRYVIFVKQGVYDETVTVTKKMANITMYGDGSQKTIVTGNKNFADGVQTFRTATFA 369
Query: 337 TVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNC 396
+ +GF+ K M F NTAG KHQAVA+RVQ D++ F +C GYQDTLYA HRQFYR+C
Sbjct: 370 VLGDGFLCKFMGFRNTAGPEKHQAVAIRVQADRAIFLNCRFEGYQDTLYAQTHRQFYRSC 429
Query: 397 EISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEP 456
I+GTVDFIFG A++V QN I VRKP NQQNI+ A G + + TG+VLQ+C I P+
Sbjct: 430 VITGTVDFIFGDATSVFQNCLITVRKPLENQQNIVTAQGRIDGHETTGIVLQSCRIEPDK 489
Query: 457 ALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTG 516
L P + K+RS+L RPWK +SR + M++TIGD I P G+LPW G L T ++AEY+N G
Sbjct: 490 DLVPVKNKIRSYLGRPWKEFSRTVIMDSTIGDFIHPGGWLPWQGDFGLKTLYYAEYSNKG 549
Query: 517 PGSNVQARVKW-GKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
G+ AR+KW G ++ K +A K+T + +G W+ A+G P LG
Sbjct: 550 GGAQTNARIKWPGYHIIKKEEAMKFTIENFYQGD-WISASGSPVHLG 595
>emb|CAA73733.1| pectin methylesterase-like protein [Zea mays]
gi|7447384|pir||T04359 pectin methylesterase-like
protein - maize
Length = 563
Score = 429 bits (1104), Expect = e-119
Identities = 239/583 (40%), Positives = 338/583 (56%), Gaps = 47/583 (8%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADAN-------------NGGELTSHTKAVTA 50
K + G+S ILVV V +GVVA V + A N G++ V
Sbjct: 3 KALLGGLSAILVVAVVVGVVATVTRSGKKAGDNFTVPGEASLANVRQVGQVPVRAHPVQG 62
Query: 51 VCQNS--DDHKFCADTLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQS 108
V + H++ + G V AV+ ++ Q
Sbjct: 63 VVREDVVPGHQWHREPQGGVPQRGQGG--------------AGVGPDGGRAVQVDRRGQG 108
Query: 109 ----TKMALDDCKDLLEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSC 164
T+ A +DCK LLE A D+L+ +A + V + R+ DL+ WL V + +C
Sbjct: 109 SDSMTESAREDCKKLLEDAADDLRGMLEMAGGDIKV--LFSRSDDLETWLTGVMTFMDTC 166
Query: 165 LDGF-DTDGEKQVQSQLQTGSLDHVGKLTALALDVVTAITKVLAALDLDLNVKPSSRRLF 223
+DGF D + + S ++ + +L++ AL + ++ +L +DL + K S RRL
Sbjct: 167 VDGFVDEKLKADMHSVVRNAT-----ELSSNALAITNSLGGILKKMDLGMFSKDSRRRLL 221
Query: 224 --EVDEDGNPEWMSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQG 281
E DE G P WM +RKLLA +G PNA+VAKDGSG+FK++ A+++ PK HQG
Sbjct: 222 SSEQDEKGWPVWMRSPERKLLA---SGNQPKPNAIVAKDGSGQFKSIQQAVDAVPKGHQG 278
Query: 282 RYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEG 341
RYVIYVKAG+YDE + + K K NI +YGDGP ++ +TG+K+F DG+ T++TATFS A G
Sbjct: 279 RYVIYVKAGLYDEIVMVPKDKVNIFMYGDGPKQSRVTGRKSFADGITTMKTATFSVEASG 338
Query: 342 FIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGT 401
FI K M F NTAGA +HQAVALRVQGD +AF++C +QDTLY RQF+RNC +SGT
Sbjct: 339 FICKNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVQPRRQFFRNCVVSGT 398
Query: 402 VDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPD 461
+DFIFG ++ V QN I+ R+P NQQN + A G NM +G+V+QNC ++P+ L PD
Sbjct: 399 IDFIFGNSAAVFQNCLIITRRPMDNQQNSVTAHGPTDPNMKSGLVIQNCRLVPDQKLFPD 458
Query: 462 RLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNV 521
R K+ S+L RPWK +SR + ME+TI D ++P+G++PW G L T ++AEY N GPG+
Sbjct: 459 RFKIPSYLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGT 518
Query: 522 QARVKW-GKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLGF 563
RV W G V+ + +A +TA +I+G +WL TG P LGF
Sbjct: 519 SKRVNWPGFHVIGRKEAEPFTAGPFIDGAMWLKYTGAPHILGF 561
>gb|AAF26136.1| putative pectinesterase [Arabidopsis thaliana]
gi|15230019|ref|NP_187212.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 669
Score = 416 bits (1069), Expect = e-114
Identities = 225/569 (39%), Positives = 336/569 (58%), Gaps = 16/569 (2%)
Query: 2 ERKIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFC 61
+R+ V +S +L++ + + V V N D+ E+ + KAV VC +D K C
Sbjct: 12 KRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTC 71
Query: 62 ADTL--GSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALDDCKDL 119
DTL NT+DP + +K +++ + A + + +E +K+++ T+MALD CK+L
Sbjct: 72 EDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTI-MELQKDSR-TRMALDQCKEL 129
Query: 120 LEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQ 179
+++A+DEL ++S H +++ +L+ WL A +++++CL+GF G + +
Sbjct: 130 MDYALDEL-SNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ--GTQGNAGE 186
Query: 180 LQTGSLDHVGKLTALALDVVTAITKVLAALDLD-LNVKPSSRRLFEVDEDGNPEWMSGAD 238
+L +LT L +++ ++ + + + LN SRRL +G P W+
Sbjct: 187 TMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLN----SRRLLA---EGFPSWVDQRG 239
Query: 239 RKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYDEYIQI 298
RKLL + V P+ VVA+DGSG++KT+ +A+ PK +V+++KAG+Y EY+Q+
Sbjct: 240 RKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQV 299
Query: 299 DKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTAGANKH 358
+KT +++ GDGP KTII+G KN+ DG+ T +TAT + V FIAK + FENTAGA KH
Sbjct: 300 NKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKH 359
Query: 359 QAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVIQNSKI 418
QAVA+RVQ D+S FF+C GYQDTLY H+HRQF+R+C ISGT+DF+FG A+ V QN +
Sbjct: 360 QAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTL 419
Query: 419 VVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPWKAYSR 478
+VRKP NQ I A G TG V Q C I EP + +++L RPWK YSR
Sbjct: 420 LVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSR 479
Query: 479 AIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKW-GKGVLSKADA 537
I M I D +QP G+ PW G L T F++E NTGPGS + RV W G LS+ D
Sbjct: 480 TIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDI 539
Query: 538 TKYTAAQWIEGGVWLPATGIPFDLGFTKG 566
K+T AQ+I+G W+P G+P+ G G
Sbjct: 540 LKFTPAQYIQGDDWIPGKGVPYTTGLLAG 568
>dbj|BAC42986.1| putative pectinesterase [Arabidopsis thaliana]
gi|29029008|gb|AAO64883.1| At3g05610 [Arabidopsis
thaliana]
Length = 669
Score = 412 bits (1059), Expect = e-113
Identities = 223/569 (39%), Positives = 335/569 (58%), Gaps = 16/569 (2%)
Query: 2 ERKIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFC 61
+R+ V +S +L++ + + V V N D+ E+ + KAV VC +D K C
Sbjct: 12 KRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTC 71
Query: 62 ADTL--GSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALDDCKDL 119
DTL NT+DP + +K +++ + A + + +E +K+++ T+MALD CK+L
Sbjct: 72 EDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTI-MELQKDSR-TRMALDQCKEL 129
Query: 120 LEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQ 179
+++A+DEL ++S H +++ +L+ WL A +++++CL+GF G + +
Sbjct: 130 MDYALDEL-SNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ--GTQGNAGE 186
Query: 180 LQTGSLDHVGKLTALALDVVTAITKVLAALDLD-LNVKPSSRRLFEVDEDGNPEWMSGAD 238
+L +LT L +++ ++ + + + LN SRRL +G P W+
Sbjct: 187 TMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLN----SRRLLA---EGFPSWVDQRG 239
Query: 239 RKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYDEYIQI 298
RKLL + V P+ VVA+DGSG++KT+ +A+ PK +V+++KAG+Y EY+Q+
Sbjct: 240 RKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQV 299
Query: 299 DKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTAGANKH 358
+KT +++ GDGP KTII+G KN+ DG+ +TAT + V FIAK + FENTAGA KH
Sbjct: 300 NKTMSHLVFIGDGPDKTIISGNKNYKDGITAYRTATVAIVGNYFIAKNIGFENTAGAIKH 359
Query: 359 QAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVIQNSKI 418
QAVA+RVQ D+S FF+C GYQ+TLY H+HRQF+R+C ISGT+DF+FG A+ V QN +
Sbjct: 360 QAVAVRVQSDESIFFNCRFDGYQNTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTL 419
Query: 419 VVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPWKAYSR 478
+VRKP NQ I A G TG V Q C I EP + +++L RPWK YSR
Sbjct: 420 LVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSR 479
Query: 479 AIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKW-GKGVLSKADA 537
I M I D +QP G+ PW G L T F++E NTGPGS + RV W G LS+ D
Sbjct: 480 TIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDI 539
Query: 538 TKYTAAQWIEGGVWLPATGIPFDLGFTKG 566
K+T AQ+I+G W+P G+P+ G G
Sbjct: 540 LKFTPAQYIQGDDWIPGKGVPYTTGLLAG 568
>gb|AAC34241.1| putative pectinesterase [Arabidopsis thaliana]
gi|15450765|gb|AAK96654.1| putative pectinesterase
[Arabidopsis thaliana] gi|61213847|sp|O80722|PME4_ARATH
Pectinesterase-4 precursor (Pectin methylesterase 4) (PE
4) (VANGUARD1-like protein 1) (VGD1-like protein 1)
(AtPME4) gi|15226482|ref|NP_182226.1| pectinesterase
family protein [Arabidopsis thaliana]
Length = 588
Score = 408 bits (1048), Expect = e-112
Identities = 234/597 (39%), Positives = 336/597 (56%), Gaps = 51/597 (8%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCAD 63
K+ VS S++L+VGVA+GVVA + N DAN L+ KAV +CQ++ D C
Sbjct: 4 KVVVSVASILLIVGVAIGVVAFINKNG---DAN----LSPQMKAVQGICQSTSDKASCVK 56
Query: 64 TLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEF 122
TL V + DPN IKA + + + + K+ N T + V + + + K LD CK + +
Sbjct: 57 TLEPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 123 AIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQT 182
A+++L ++I+ + + + LK WL V+ YQ CLD + D ++ +
Sbjct: 117 ALEDL--ATIIEEMGEDLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDDLRKAIGE--- 171
Query: 183 GSLDHVGKLTALALD----VVTAITKVLAALDLDLNVK---------------------- 216
+ + LT A+D VV+A+ K+ +D N+
Sbjct: 172 -GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPDG 230
Query: 217 PSSRRLFEVDEDGNPEWMSGADRKLLADMSTGMS--------VTPNAVVAKDGSGKFKTV 268
P+ R L ++DE G P W+SGADRKL+A G V N VVAKDGSG+FKTV
Sbjct: 231 PARRLLEDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQFKTV 290
Query: 269 LDAINSYPKNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFV--DG 326
A+++ P+N++GR +IY+KAG+Y E + I K K NI ++GDG KT+I+ ++ G
Sbjct: 291 QQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVALSRG 350
Query: 327 VKTIQTATFSTVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYA 386
T +AT +EGF+AK M F+NTAG HQA A+RV GD++ F+C GYQDTLY
Sbjct: 351 TTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTLYV 410
Query: 387 HAHRQFYRNCEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGT-VQKNMPTGV 445
+ RQFYRNC +SGTVDFIFG ++TVIQN+ IVVRK Q N + ADG + M G+
Sbjct: 411 NNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKIGI 470
Query: 446 VLQNCEIMPEPALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLD 505
VLQNC I+P+ L P+RL V ++L RPWK +S + M +GDLI+P+G+ W G F
Sbjct: 471 VLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESFHK 530
Query: 506 TCFFAEYANTGPGSNVQARVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
+C + EY N GPG+ RV W K S A+ +TAA W+ W+ +P +G
Sbjct: 531 SCRYVEYNNRGPGAFANRRVNWAKVARSAAEVNGFTAANWLGPINWIQEANVPVTIG 587
>gb|AAC27719.1| flower-specific pectin methylesterase precursor [Arabidopsis
thaliana]
Length = 586
Score = 405 bits (1042), Expect = e-111
Identities = 231/595 (38%), Positives = 335/595 (55%), Gaps = 49/595 (8%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCAD 63
K+ VS S++L+VGVA+GVVA + N DAN L+ KAV +CQ++ D C
Sbjct: 4 KVVVSVASILLIVGVAIGVVAFINKNG---DAN----LSPQMKAVQGICQSTSDKASCVK 56
Query: 64 TLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEF 122
TL V + DPN IKA + + + + K+ N T + V + + + K LD CK + +
Sbjct: 57 TLEPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 123 AIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQT 182
A+++L ++I+ + + + LK WL V+ YQ CLD + D ++ +
Sbjct: 117 ALEDL--ATIIEEMGEDLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDDLRKAIGE--- 171
Query: 183 GSLDHVGKLTALALD----VVTAITKVLAALDLDLNVK---------------------- 216
+ + LT A+D VV+A+ K+ +D N+
Sbjct: 172 -GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPDG 230
Query: 217 PSSRRLFEVDEDGNPEWMSGADRKLLADMSTGMS--------VTPNAVVAKDGSGKFKTV 268
P+ R L ++DE G P W+SGADRKL+A G V N VVAKDGSG+FKTV
Sbjct: 231 PARRLLEDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQFKTV 290
Query: 269 LDAINSYPKNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVK 328
A+++ P+N++GR +IY+KAG+Y E + I K K NI ++GDG KT+I+ ++
Sbjct: 291 QQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVALSRG 350
Query: 329 TIQTATFSTVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHA 388
T + + + +EGF+AK M F+NTAG HQA A+RV GD++ F+C GYQDTLY +
Sbjct: 351 TTTSLSATVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTLYVNN 410
Query: 389 HRQFYRNCEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGT-VQKNMPTGVVL 447
RQFYRNC +SGTVDFIFG ++TVIQN+ IVVRK Q N + ADG + M G+VL
Sbjct: 411 GRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKIGIVL 470
Query: 448 QNCEIMPEPALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTC 507
QNC I+P+ L P+RL V ++L RPWK +S + M +GDLI+P+G+ W G F +C
Sbjct: 471 QNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESFHKSC 530
Query: 508 FFAEYANTGPGSNVQARVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
+ EY N GPG+ RV W K S A+ +TAA W+ W+ +P +G
Sbjct: 531 RYVEYNNRGPGAFANRRVNWAKVARSAAEVNGFTAANWLGPINWIQEANVPVTIG 585
>ref|NP_198139.1| pectinesterase family protein [Arabidopsis thaliana]
Length = 732
Score = 405 bits (1040), Expect = e-111
Identities = 223/569 (39%), Positives = 340/569 (59%), Gaps = 20/569 (3%)
Query: 2 ERKIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFC 61
+++ + +S +L++ + + V V N +D E+T+ KA+ VC +D + C
Sbjct: 13 KKRYVIISISSVLLISMVVAVTIGVSVNK--SDNAGDEEITTSVKAIKDVCAPTDYKETC 70
Query: 62 ADTL--GSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALDDCKDL 119
DTL + +TSDP + +K +++ + + + +E +K+ ++ KMALD CK+L
Sbjct: 71 EDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTM-IELQKDPRA-KMALDQCKEL 128
Query: 120 LEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQ 179
+++AI EL + S H V++ L+ WL A +++Q+CLDGF G + +
Sbjct: 129 MDYAIGEL-SKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ--GTQGNAGE 185
Query: 180 LQTGSLDHVGKLTALALDVVTAITKVLAALDLDLNVKPSSRRLFEVDEDGNPEWMSGADR 239
+L +LT L +VT ++ L + + + +SRRL + P WM R
Sbjct: 186 TIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLSQEF---PSWMDARAR 239
Query: 240 KLL-ADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYDEYIQI 298
+LL A MS V P+ VVA+DGSG++KT+ +A+N PK +V+++K G+Y EY+Q+
Sbjct: 240 RLLNAPMS---EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQV 296
Query: 299 DKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTAGANKH 358
+++ +++ GDGP KT+I+G K++ DG+ T +TAT + V + FIAK +AFENTAGA KH
Sbjct: 297 NRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKH 356
Query: 359 QAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVIQNSKI 418
QAVA+RV D+S F++C GYQDTLYAH+HRQFYR+C ISGT+DF+FG A+ V QN +
Sbjct: 357 QAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTL 416
Query: 419 VVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPWKAYSR 478
+VRKP NQ I A G TG VLQ C I+ EP + + +++L RPWK YSR
Sbjct: 417 LVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSR 476
Query: 479 AIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKW-GKGVLSKADA 537
I M I D + P+G+ PW G L+T F++E NTGPG+ + RV W G LS +
Sbjct: 477 TIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEI 536
Query: 538 TKYTAAQWIEGGVWLPATGIPFDLGFTKG 566
K+T AQ+I+G W+P G+P+ LG G
Sbjct: 537 LKFTPAQYIQGDAWIPGKGVPYILGLFSG 565
>gb|AAV91509.1| VGD1-like protein 1 [Arabidopsis thaliana]
Length = 588
Score = 403 bits (1036), Expect = e-111
Identities = 231/597 (38%), Positives = 335/597 (55%), Gaps = 51/597 (8%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCAD 63
K+ VS S++L+VGVA+GVVA + N DAN L+ KAV +C+++ D C
Sbjct: 4 KVVVSVASILLIVGVAIGVVAFINKNG---DAN----LSPQMKAVQGICRSTSDKASCVK 56
Query: 64 TLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEF 122
TL V + DPN IKA + + + + K+ N T + V + + + K LD CK + +
Sbjct: 57 TLEPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 123 AIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQT 182
A+++L ++I+ + + + LK WL V+ YQ CLD + D ++ +
Sbjct: 117 ALEDL--ATIIEEMGEDLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDDLRKAIGE--- 171
Query: 183 GSLDHVGKLTALALD----VVTAITKVLAALDLDLNVK---------------------- 216
+ + LT A+D VV+A+ K+ +D N+
Sbjct: 172 -GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPDG 230
Query: 217 PSSRRLFEVDEDGNPEWMSGADRKLLADMSTGMS--------VTPNAVVAKDGSGKFKTV 268
P+ R L ++DE G P W+SGADRKL+A G V N VVAKDGSG+FKTV
Sbjct: 231 PARRLLEDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRANFVVAKDGSGQFKTV 290
Query: 269 LDAINSYPKNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFV--DG 326
A+++ P+N++GR +IY+KAG+Y E + I K NI ++GDG KT+I+ ++ G
Sbjct: 291 QQAVDACPENNRGRCIIYIKAGLYREQVIIPKKNNNIFMFGDGARKTVISYNRSVALSRG 350
Query: 327 VKTIQTATFSTVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYA 386
T +AT +EGF+AK M F+NTAG HQA A+RV GD++ F+C GYQDTLY
Sbjct: 351 TTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTLYV 410
Query: 387 HAHRQFYRNCEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGT-VQKNMPTGV 445
+ RQFYRNC +SGTVDFIFG ++TVIQN+ IVVRK Q N + ADG + M G+
Sbjct: 411 NNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKIGI 470
Query: 446 VLQNCEIMPEPALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLD 505
VLQNC I+P+ L P+RL V ++L RPWK +S + M +GDLI+P+G+ W G F
Sbjct: 471 VLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESFHK 530
Query: 506 TCFFAEYANTGPGSNVQARVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
+C + EY N GPG+ R W K S A+ + +TAA W+ W+ +P +G
Sbjct: 531 SCRYVEYNNRGPGAFANRRANWAKVARSAAEVSGFTAANWLGPINWIQEANVPVTIG 587
>emb|CAA39658.1| Bp19 [Brassica napus] gi|99823|pir||S14952 pectinesterase homolog -
rape gi|1172538|sp|P41510|PME_BRANA Probable
pectinesterase precursor (Pectin methylesterase) (PE)
Length = 584
Score = 400 bits (1028), Expect = e-110
Identities = 228/588 (38%), Positives = 330/588 (55%), Gaps = 38/588 (6%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCAD 63
KI +S S++LVVGVA+GVV V G A L SH KAV ++C ++ D CA
Sbjct: 5 KIVISVASMLLVVGVAIGVVTFVNKGGG---AGGDKTLNSHQKAVESLCASATDKGSCAK 61
Query: 64 TLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEF 122
TL V + DP+ IKA + + ++V K+ N T KN N ++K LD CK +L +
Sbjct: 62 TLDPVKSDDPSKLIKAFMLATKDAVTKSTNFTASTEEGMGKNINATSKAVLDYCKRVLMY 121
Query: 123 AIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQT 182
A+++L+ +I+ + + LK WL VF YQ C+D + ++V +
Sbjct: 122 ALEDLE--TIVEEMGEDLQQSGSKMDQLKQWLTGVFNYQTDCIDDIEESELRKVMGE--- 176
Query: 183 GSLDHVGKLTALALDVVTAITKVLAALDL--------DLNVKPSSRR--LFEVDEDGNPE 232
+ H L++ A+D+ A+T ++ +++ +L P+ R L ++D+ G P+
Sbjct: 177 -GIAHSKILSSNAIDIFHALTTAMSQMNVKVDDMKKGNLGETPAPDRDLLEDLDQKGLPK 235
Query: 233 WMSGADRKLLADMST---------------GMSVTPNAVVAKDGSGKFKTVLDAINSYPK 277
W S DRKL+A G + P VVAKDGSG+FKT+ +A+ + P+
Sbjct: 236 WHSDKDRKLMAQAGRPGAPADEGIGEGGGGGGKIKPTHVVAKDGSGQFKTISEAVKACPE 295
Query: 278 NHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKN--FVDGVKTIQTATF 335
+ GR +IY+KAGVY E + I K N+ ++GDG T+TIIT ++ G T + T
Sbjct: 296 KNPGRCIIYIKAGVYKEQVTIPKKVNNVFMFGDGATQTIITFDRSVGLSPGTTTSLSGTV 355
Query: 336 STVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRN 395
+EGF+AK + F+NTAG HQAVA RV GD++ F+C GYQDTLY + RQFYRN
Sbjct: 356 QVESEGFMAKWIGFQNTAGPLGHQAVAFRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRN 415
Query: 396 CEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQ-KNMPTGVVLQNCEIMP 454
+SGTVDFIFG ++TVIQNS I+ RK Q N + ADG + K + G+VL NC IM
Sbjct: 416 IVVSGTVDFIFGKSATVIQNSLILCRKGSPGQTNHVTADGNEKGKAVKIGIVLHNCRIMA 475
Query: 455 EPALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYAN 514
+ L+ DRL V+S+L RPWK ++ + IGDLIQP G+ W G +F T + E+ N
Sbjct: 476 DKELEADRLTVKSYLGRPWKPFATTAVIGTEIGDLIQPTGWNEWQGEKFHLTATYVEFNN 535
Query: 515 TGPGSNVQARVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
GPG+N ARV W K S A+ ++T A W+ W+ +P LG
Sbjct: 536 RGPGANTAARVPWAKMAKSAAEVERFTVANWLTPANWIQEANVPVQLG 583
>emb|CAB71877.1| PECTINESTERASE-like protein [Arabidopsis thaliana]
gi|21536813|gb|AAM61145.1| PECTINESTERASE-like protein
[Arabidopsis thaliana] gi|20260676|gb|AAM13236.1|
pectinesterase-like protein [Arabidopsis thaliana]
gi|61213924|sp|Q5MFV6|PMEL_ARATH Probable pectinesterase
VGDH2 precursor (Pectin methylesterase) (PE)
(VANGUARD1-like protein 2) (VGD1-like protein 2)
gi|15228697|ref|NP_191776.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 588
Score = 399 bits (1025), Expect = e-109
Identities = 228/590 (38%), Positives = 333/590 (55%), Gaps = 38/590 (6%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCAD 63
K+ VS SL+LVVGVA+GV+ V G A+ ++ G + SH KAV +CQ++ D CA
Sbjct: 5 KVVVSVASLLLVVGVAIGVITFVNKGGG-ANGDSNGPINSHQKAVQTICQSTTDQGSCAK 63
Query: 64 TLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEF 122
TL V + DP+ +KA + + +++ K+ N T N N ++K LD CK +L +
Sbjct: 64 TLDPVKSDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRVLMY 123
Query: 123 AIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQT 182
A+++L+ +I+ + + LK WL VF YQ CLD + K++ +
Sbjct: 124 ALEDLE--TIVEEMGEDLQQSGTKLDQLKQWLTGVFNYQTDCLDDIEEVELKKIMGE--- 178
Query: 183 GSLDHVGKLTALALD----VVTAITKVLAALDLDLNVKP------SSRRLFEV-DEDGNP 231
+ + LT+ A+D VVTA+ ++ +D N+ ++RRL E D G P
Sbjct: 179 -GISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDNDSKGLP 237
Query: 232 EWMSGADRKLLADMSTGM----------------SVTPNAVVAKDGSGKFKTVLDAINSY 275
+W SG DRKL+A G + VVAKDGSG+FKT+ +A+ +
Sbjct: 238 KWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEAVMAC 297
Query: 276 PKNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKN--FVDGVKTIQTA 333
P + GR +I++KAG+Y+E ++I K K NI ++GDG T+TIIT ++ G T +
Sbjct: 298 PDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPGTTTSLSG 357
Query: 334 TFSTVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFY 393
T +EGF+AK + F+NTAG HQAVALRV GD++ F+C GYQDTLY + RQFY
Sbjct: 358 TVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYVNNGRQFY 417
Query: 394 RNCEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQ-KNMPTGVVLQNCEI 452
RN +SGTVDFIFG ++TVIQNS I+VRK Q N + ADG + M G+VL NC I
Sbjct: 418 RNIVVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNYVTADGNEKGAAMKIGIVLHNCRI 477
Query: 453 MPEPALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEY 512
+P+ L+ D+L ++S+L RPWK ++ + + IGDLI+P+G+ W G Q T + E+
Sbjct: 478 IPDKELEADKLTIKSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQNHKTAKYIEF 537
Query: 513 ANTGPGSNVQARVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
N GPG+ R W K S A+ YT A W+ W+ +P LG
Sbjct: 538 NNRGPGAATTQRPPWVKVAKSAAEVETYTVANWVGPANWIQEANVPVQLG 587
>dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
Length = 617
Score = 399 bits (1024), Expect = e-109
Identities = 228/556 (41%), Positives = 319/556 (57%), Gaps = 46/556 (8%)
Query: 34 DANNGG---ELTSHTKAVTAVCQNSDDHKFCADTLG---SVNTSDPNDYIKAVVKTSIES 87
D GG +L + +K++ +C +D CA ++G + + S P D I+ V +
Sbjct: 80 DGGGGGGKADLRAVSKSIKMMCAQTDFADSCATSIGKAANASVSSPKDIIRTAVDVIGGA 139
Query: 88 VIKAFNMTDKLAVENEKNNQSTKMALDDCKDLLEFAIDELQASSILAADNSSVHNVNDRA 147
V +AF+ D + N+ K A+ DCK+L + A D+L + + D +
Sbjct: 140 VDQAFDRADLIM----SNDPRVKAAVADCKELFDDAKDDLNCT-LKGIDGKDGLK---QG 191
Query: 148 ADLKNWLGAVFAYQQSCLDGFDTDGE--KQVQSQLQTGSLDHVGKLTALALDVVTAITKV 205
L+ WL AV A ++C+DGF DGE +V+ G + T+ AL ++ +
Sbjct: 192 FQLRVWLSAVIANMETCIDGFP-DGEFRDKVKESFNNGR-----EFTSNALALIEKASSF 245
Query: 206 LAALDLDLNVKPSSRRLFEVDED---------------GNPEWMSGADRKLLADMSTGMS 250
L+AL K S RRL +ED G PEW+ DR++L +
Sbjct: 246 LSAL------KGSQRRLLAGEEDNGGGAADPHLALAEDGIPEWVPDGDRRVLKGGGFKNN 299
Query: 251 VTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGD 310
+TPN +VAKDGSGKFKT+ +A+ + PK + GRYVIYVK GVY EY+ I K ++ +YGD
Sbjct: 300 LTPNVIVAKDGSGKFKTINEALAAMPKTYSGRYVIYVKEGVYAEYVTITKKMASVTMYGD 359
Query: 311 GPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTAGANKHQAVALRVQGDKS 370
G K+I+TG KNF DG+ T +TATF+ +GF+A M F+NTAGA KHQAVAL VQ DKS
Sbjct: 360 GSRKSIVTGSKNFADGLTTFKTATFAAQGDGFMAIGMGFQNTAGAAKHQAVALLVQSDKS 419
Query: 371 AFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNI 430
F +C + G+QDTLYAH+ QFYRNC I+GT+DF+FG A+ V QN + +R+P NQQNI
Sbjct: 420 VFLNCWMDGFQDTLYAHSKAQFYRNCVITGTIDFVFGDAAAVFQNCVLTLRRPMDNQQNI 479
Query: 431 IVADGTVQKNMPTGVVLQNCEIMPEPALQPDRL-KVRSFLARPWKAYSRAIFMENTIGDL 489
A G TG VLQ CE EPAL +L +R++L RPW+ +SR + ME+ I +
Sbjct: 480 ATAQGRADGREATGFVLQKCEFNAEPALTDAKLPPIRNYLGRPWREFSRTVIMESDIPAI 539
Query: 490 IQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKWG--KGVLSKADATKYTAAQWIE 547
I G++PW G L T ++AEYAN GPG++ RV W K V+SKADATK+T ++
Sbjct: 540 IDKAGYMPWNGEFALKTLYYAEYANKGPGADTAGRVAWPGYKKVISKADATKFTVDNFLH 599
Query: 548 GGVWLPATGIPFDLGF 563
W+ TG P F
Sbjct: 600 AKPWIDPTGTPVKYDF 615
>gb|AAV91510.1| VGD1-like protein 2 [Arabidopsis thaliana]
Length = 588
Score = 394 bits (1011), Expect = e-108
Identities = 226/590 (38%), Positives = 331/590 (55%), Gaps = 38/590 (6%)
Query: 4 KIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCAD 63
K+ VS SL+LVVGVA+GV+ V G A+ ++ G + SH KAV +C ++ D CA
Sbjct: 5 KVVVSVASLLLVVGVAIGVITFVNKGGG-ANGDSNGPINSHQKAVQTICLSTTDQGSCAK 63
Query: 64 TLGSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEF 122
TL V + DP+ +KA + + +++ K+ N T N N ++K LD CK +L +
Sbjct: 64 TLDPVKSDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRVLMY 123
Query: 123 AIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQT 182
A+++L+ +I+ + + LK WL VF YQ CL + K++ +
Sbjct: 124 ALEDLE--TIVEEMGEDLQQSGTKLDQLKQWLTGVFNYQTDCLGDIEEVELKKIMGE--- 178
Query: 183 GSLDHVGKLTALALD----VVTAITKVLAALDLDLNVKP------SSRRLFEV-DEDGNP 231
+ + LT+ A+D VVTA+ ++ +D N+ ++RRL E D G P
Sbjct: 179 -GISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDNDSKGLP 237
Query: 232 EWMSGADRKLLADMSTGM----------------SVTPNAVVAKDGSGKFKTVLDAINSY 275
+W SG DRKL+A G + VVAKDGSG+FKT+ +A+ +
Sbjct: 238 KWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEAVMAC 297
Query: 276 PKNHQGRYVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKN--FVDGVKTIQTA 333
P + GR +I++KAG+Y+E ++I K K NI ++GDG T+TIIT ++ G T +
Sbjct: 298 PDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPGTTTSLSG 357
Query: 334 TFSTVAEGFIAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFY 393
T +EGF+AK + F+NTAG HQAVALRV GD++ F+C GYQDTLY + RQFY
Sbjct: 358 TVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYVNNGRQFY 417
Query: 394 RNCEISGTVDFIFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQ-KNMPTGVVLQNCEI 452
RN +SGTVDFIFG ++TVIQNS I+VRK Q N + ADG + M G+VL NC I
Sbjct: 418 RNIVVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNYVTADGNEKGAAMKIGIVLHNCRI 477
Query: 453 MPEPALQPDRLKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEY 512
+P+ L+ D+L ++S+L RPWK ++ + + IGDLI+P+G+ W G Q T + E+
Sbjct: 478 IPDKELEADKLTIKSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQNHKTAKYIEF 537
Query: 513 ANTGPGSNVQARVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
N GPG+ R W K S A+ YT A W+ W+ +P LG
Sbjct: 538 NNRGPGAATTQRPPWVKVAKSAAEVETYTVANWVGPANWIQEANVPVQLG 587
>ref|XP_479611.1| putative pectin methylesterase [Oryza sativa (japonica
cultivar-group)] gi|34394412|dbj|BAC83510.1| putative
pectin methylesterase [Oryza sativa (japonica
cultivar-group)]
Length = 566
Score = 390 bits (1001), Expect = e-107
Identities = 225/566 (39%), Positives = 316/566 (55%), Gaps = 32/566 (5%)
Query: 7 VSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCADTLG 66
+ G S +LVV V V + N G GEL++ K+V A CQ +D + C + LG
Sbjct: 6 IIGASTVLVVAVVAAVCVVSFKNGG--GGKEDGELSTSVKSVKAFCQPTDYQQTCEEELG 63
Query: 67 SV---NTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALDDCKDLLEFA 123
S P D KA+ + E + KA + + L E KN++ T AL +CK+LLE+A
Sbjct: 64 KAAGNGASSPTDLAKAMFAVTSEKISKAISESSTL--EELKNDKRTSGALQNCKELLEYA 121
Query: 124 IDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGF---DTDGEKQVQSQL 180
+D+L+ +S + N + DL+ WL A YQ +CLDGF TD +++S L
Sbjct: 122 VDDLK-TSFEKLGGFEMTNFHKAVDDLRTWLSAALTYQGTCLDGFLNTTTDAADKMKSAL 180
Query: 181 QTGSLDHVGKLTALALDVVTAITKVLAALDLDLNVKPSSRRLFEVDEDGNPEWMSGADRK 240
+ +LT L VV + L +L++ RRL +DG P WMS R+
Sbjct: 181 NSSQ-----ELTEDILAVVDQFSATLGSLNI------GRRRLLA--DDGMPVWMSEGGRR 227
Query: 241 LLADMSTG----MSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYDEYI 296
L + + + P+ VA DGSG KT+ +A+ P ++ RY IYVKAG Y+EY+
Sbjct: 228 QLLEAAGPEAGPVEFKPDVTVAADGSGDVKTIGEAVAKVPPKNKERYTIYVKAGTYNEYV 287
Query: 297 QIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTAGAN 356
+ + N+ + GDG KTIITG KNF + T TAT + GF + + ENTAG
Sbjct: 288 SVGRPATNVNMIGDGIGKTIITGNKNFKMNLTTKDTATMEAIGNGFFMRGITVENTAGPE 347
Query: 357 KHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVIQNS 416
HQAVALR Q D + F+ C GYQDTLY HA RQF+R+C +SGT+DFIFG + V+QN
Sbjct: 348 NHQAVALRAQSDMAVFYQCEFDGYQDTLYPHAQRQFFRDCTVSGTIDFIFGNSQVVLQNC 407
Query: 417 KIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPWKAY 476
+ RKP NQ NII A G +K G V+ NC + P P L+ KV+++LARPWK Y
Sbjct: 408 LLQPRKPMDNQVNIITAQGRREKRSAGGTVIHNCTVAPHPDLEKFTDKVKTYLARPWKEY 467
Query: 477 SRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKWGKGV--LSK 534
SR IF++N IG ++ P G+L W G LDT ++AE N GPG+++ R KW KGV L+
Sbjct: 468 SRTIFVQNEIGAVVDPVGWLEWNGNFALDTLYYAEVDNHGPGADMSKRAKW-KGVQSLTY 526
Query: 535 ADATK-YTAAQWIEGGVWLPATGIPF 559
D K +T +I+G ++P G+P+
Sbjct: 527 QDVQKEFTVEAFIQGQEFIPKFGVPY 552
>gb|AAM65978.1| pectin methylesterase [Arabidopsis thaliana]
gi|10177147|dbj|BAB10336.1| pectin methylesterase
[Arabidopsis thaliana] gi|18700151|gb|AAL77687.1|
AT5g49180/K21P3_5 [Arabidopsis thaliana]
gi|15239814|ref|NP_199729.1| pectinesterase family
protein [Arabidopsis thaliana]
gi|25090082|gb|AAN72223.1| At5g49180/K21P3_5
[Arabidopsis thaliana]
Length = 571
Score = 389 bits (998), Expect = e-106
Identities = 223/565 (39%), Positives = 323/565 (56%), Gaps = 14/565 (2%)
Query: 2 ERKIFVSGVSLILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFC 61
++K ++GV L+V + + V N ++ ++ + T AV AVC +D + C
Sbjct: 9 KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETC 68
Query: 62 ADTL--GSVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALDDCKDL 119
++L S +++ P D IK +I S+ + N++ TK AL+ C+ L
Sbjct: 69 VNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKL 128
Query: 120 LEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQ 179
+ A D+L+ + D S+ + D DL+ WL AYQQ+C+D F+ K Q
Sbjct: 129 MNDATDDLK-KCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDM 187
Query: 180 LQTGSLDHVGKLTALALDVVTAITKVLAALDLDL---NVKPSSRRLFEVDEDGNPEWMSG 236
+ +LT+ L ++T I+ +L ++ ++ +R+L EDG P W+
Sbjct: 188 QKIFKTSR--ELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA-EDGIPSWVGP 244
Query: 237 ADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYDEYI 296
R+L+A T V N VVA DGSG++KT+ +A+N+ PK +Q +VIY+K GVY+E +
Sbjct: 245 NTRRLMA---TKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKV 301
Query: 297 QIDKTKKNILIYGDGPTKTIITGKKNFVDG-VKTIQTATFSTVAEGFIAKAMAFENTAGA 355
+ K ++ GDGPTKT ITG N+ G VKT TAT + + F AK + FENTAG
Sbjct: 302 DVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGP 361
Query: 356 NKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVIQN 415
HQAVALRV D + F++C I GYQDTLY H+HRQF+R+C +SGTVDFIFG V+QN
Sbjct: 362 EGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQN 421
Query: 416 SKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPWKA 475
IVVRKP +Q +I A G K TG+VLQNC I EPA P + +++L RPWK
Sbjct: 422 CNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKE 481
Query: 476 YSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKW-GKGVLSK 534
+SR I M TI D+I P G+LPW G L+T ++AEY N GPGSN RVKW G LS
Sbjct: 482 FSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSP 541
Query: 535 ADATKYTAAQWIEGGVWLPATGIPF 559
A ++T A+++ G +W+P +P+
Sbjct: 542 KQALRFTPARFLRGNLWIPPNRVPY 566
>gb|AAF23892.1| pectin methyl esterase [Solanum tuberosum]
Length = 576
Score = 385 bits (990), Expect = e-105
Identities = 220/571 (38%), Positives = 328/571 (56%), Gaps = 19/571 (3%)
Query: 2 ERKIFVSGVSLILVVGVALGVVALV--RTNNGPADANNGGELTSHTKAVTAVCQNSDDHK 59
++KI+++ V+ +L+V +GVVA V R+NN DA+ +S V + C N+ +
Sbjct: 14 KKKIYLAIVASVLLVAAVIGVVAGVKSRSNNSDDDADIMAISSSAHAIVKSACSNTLHPE 73
Query: 60 FCADTLGSVN-----TSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALD 114
C + +V + D I+ + ++++V + + +L + K+AL
Sbjct: 74 LCYSAIVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALH 133
Query: 115 DCKDLLEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTD-GE 173
DC + ++ +DEL + + ++ + A DLK + + Q++CLDGF D +
Sbjct: 134 DCLETMDETLDELHTAVADLELYPNKKSLKEHAEDLKTLISSAITNQETCLDGFSHDEAD 193
Query: 174 KQVQSQLQTGSLDHVGKLTALALDVVTAITKVLAALDLDLNVKPSSRRLFEVDEDGNPEW 233
K+V+ L G HV K+ + AL ++ +T A ++ L+ SR+L E D PEW
Sbjct: 194 KKVRKVLLKGQ-KHVEKMCSNALAMICNMTNTDIANEMKLS---GSRKLVE-DNGEWPEW 248
Query: 234 MSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYD 293
+S DR+LL + VTP+ VVA DGSG +KTV +A+ P+ RYVI +KAGVY
Sbjct: 249 LSAGDRRLLQSST----VTPDVVVAADGSGDYKTVSEAVAKAPEKSSKRYVIRIKAGVYR 304
Query: 294 EYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTA 353
E + + K K NI+ GDG + TIIT +N DG T +AT + V E F+A+ + F+NTA
Sbjct: 305 ENVDVPKKKTNIMFMGDGRSNTIITASRNVQDGSTTFHSATVAAVGEKFLARDITFQNTA 364
Query: 354 GANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVI 413
GA+KHQAVALRV D SAF+ C I YQDTLY H++RQF+ C ++GTVDFIFG + V+
Sbjct: 365 GASKHQAVALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVL 424
Query: 414 QNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPW 473
Q+ I R+P + Q+N++ A G N TG+V+Q C I L+P + ++L RPW
Sbjct: 425 QDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPW 484
Query: 474 KAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKW--GKGV 531
K YSR + M+++I D+IQP G+ W G L+T F+ EYANTG G+ RVKW K +
Sbjct: 485 KEYSRTVIMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGAATSGRVKWKGHKVI 544
Query: 532 LSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
S +A YT +I GG WL +TG PF LG
Sbjct: 545 TSSTEAQAYTPGSFIAGGSWLSSTGFPFSLG 575
>pir||T52325 pectinesterase (EC 3.1.1.11) [imported] - turnip (fragment)
gi|1448939|gb|AAB04617.1| pectinesterase
gi|6093741|sp|Q42608|PME_BRACM Pectinesterase (Pectin
methylesterase) (PE)
Length = 571
Score = 383 bits (983), Expect = e-104
Identities = 221/579 (38%), Positives = 322/579 (55%), Gaps = 38/579 (6%)
Query: 13 ILVVGVALGVVALVRTNNGPADANNGGELTSHTKAVTAVCQNSDDHKFCADTLGSVNTSD 72
+LVVGVA+GVV V G A L SH KAV ++C ++ D CA TL V + D
Sbjct: 1 LLVVGVAIGVVTFVNKGGG---AGGDKTLNSHQKAVESLCASATDKGSCAKTLDPVKSDD 57
Query: 73 PNDYIKAVVKTSIESVIKAFNMTDKLAVENEKN-NQSTKMALDDCKDLLEFAIDELQASS 131
P+ IKA + + ++V K+ N T KN N ++K LD CK +L +A+++L+ +
Sbjct: 58 PSKLIKAFMLATKDAVTKSTNFTASTEEGMGKNMNATSKAVLDYCKRVLMYALEDLE--T 115
Query: 132 ILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFDTDGEKQVQSQLQTGSLDHVGKL 191
I+ + + LK WL VF YQ C+D + ++V + + H L
Sbjct: 116 IVEEMGEDLQQSGSKMDQLKQWLTGVFNYQTDCIDDIEESELRKVMGE----GIRHSKIL 171
Query: 192 TALALDVVTAITKVLAALDL--------DLNVKPSSRR--LFEVDEDGNPEWMSGADRKL 241
++ A+D+ A+T ++ +++ +L P+ R L ++D+ G P+W S DRKL
Sbjct: 172 SSNAIDIFHALTTAMSQMNVKVDDMKKGNLGETPAPDRDLLEDLDQKGLPKWHSDKDRKL 231
Query: 242 LADMST---------------GMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIY 286
+A G + P VVAKDGSG+FKT+ +A+ + P+ + GR +IY
Sbjct: 232 MAQAGRPGAPADEGIGEGGGGGGKIKPTHVVAKDGSGQFKTISEAVKACPEKNPGRCIIY 291
Query: 287 VKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKN--FVDGVKTIQTATFSTVAEGFIA 344
+KAGVY E + I K N+ ++GDG T+TIIT ++ G T + T +EGF+A
Sbjct: 292 IKAGVYKEQVTIPKKVNNVFMFGDGATQTIITFDRSVGLSPGTTTSLSGTVQVESEGFMA 351
Query: 345 KAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDF 404
K + F+NTAG +QAVA RV GD++ F+C GYQDTLY + RQFYRN +SGTVDF
Sbjct: 352 KWIGFQNTAGPLGNQAVAFRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNIVVSGTVDF 411
Query: 405 IFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQ-KNMPTGVVLQNCEIMPEPALQPDRL 463
I G ++TVIQNS I+ RK Q N + ADG + K + G+VL NC IM + L+ DRL
Sbjct: 412 INGKSATVIQNSLILCRKGSPGQTNHVTADGKQKGKAVKIGIVLHNCRIMADKELEADRL 471
Query: 464 KVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQA 523
V+S+L RPWK ++ + IGDLIQP G+ W G +F T + E+ N GPG+N A
Sbjct: 472 TVKSYLGRPWKPFATTAVIGTEIGDLIQPTGWNEWQGEKFHLTATYVEFNNRGPGANPAA 531
Query: 524 RVKWGKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
RV W K S A+ ++T A W+ W+ + LG
Sbjct: 532 RVPWAKMAKSAAEVERFTVANWLTPANWIQEANVTVQLG 570
>gb|AAP40488.1| putative pectin methylesterase [Arabidopsis thaliana]
Length = 614
Score = 382 bits (980), Expect = e-104
Identities = 211/522 (40%), Positives = 319/522 (60%), Gaps = 23/522 (4%)
Query: 46 KAVTAVCQNSDDHKFCADTLGSVNTSDPN---DYIKAVVKTSIESVIKAFNMTDKLAVEN 102
K + +C ++ + C TL N +D D +K++IE+V + ++ + +
Sbjct: 107 KIIQTLCSSTLYMQICEKTLK--NRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSL 164
Query: 103 EKNNQSTKMALDDCKDLLEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQ 162
+ NQ K A++ CK L+E A +E AS + + + V++ DL++WL AV +YQ+
Sbjct: 165 KTENQDDKDAIEQCKLLVEDAKEETVAS-LNKINVTEVNSFEKVVPDLESWLSAVMSYQE 223
Query: 163 SCLDGFDTDGEKQVQSQLQTGSLDHVGKLTALALDVVTAITKVLAALDLDLNVKPSSRRL 222
+CLDGF+ E ++S+++T S++ LT+ +L ++ T+ L+ + +K R L
Sbjct: 224 TCLDGFE---EGNLKSEVKT-SVNSSQVLTSNSLALIKTFTENLSPV-----MKVVERHL 274
Query: 223 FEVDEDGNPEWMSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGR 282
DG P W+S DR++L + ++ PNA VAKDGSG F T+ DA+ + P+ ++GR
Sbjct: 275 L----DGIPSWVSNDDRRMLRAVDV-KALKPNATVAKDGSGDFTTINDALRAMPEKYEGR 329
Query: 283 YVIYVKAGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGF 342
Y+IYVK G+YDEY+ +DK K N+ + GDG KTI+TG K+ ++T TATF EGF
Sbjct: 330 YIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGF 389
Query: 343 IAKAMAFENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTV 402
+A++M F NTAG+ HQAVA+RVQ D+S F +C GYQDTLYA+ HRQ+YR+C I GT+
Sbjct: 390 MAQSMGFRNTAGSEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTI 449
Query: 403 DFIFGYASTVIQNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDR 462
DFIFG A+ + QN I +RK Q+N + A G V K TG V+ NC+I L+P +
Sbjct: 450 DFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVK 509
Query: 463 LKVRSFLARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQF-LDTCFFAEYANTGPGSNV 521
+ +S+L RPWK YSR I ME+ I ++I P G+L W T F +DT ++AEY N G +
Sbjct: 510 EEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDT 569
Query: 522 QARVKW-GKGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
+RVKW G V++K +A YT +++G W+ A+G P LG
Sbjct: 570 TSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLG 610
>gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis thaliana] gi|9279579|dbj|BAB01037.1|
pectinesterase [Arabidopsis thaliana]
gi|15529256|gb|AAK97722.1| AT3g14310/MLN21_9
[Arabidopsis thaliana] gi|14335010|gb|AAK59769.1|
AT3g14310/MLN21_9 [Arabidopsis thaliana]
gi|15231828|ref|NP_188048.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 592
Score = 382 bits (980), Expect = e-104
Identities = 224/576 (38%), Positives = 327/576 (55%), Gaps = 30/576 (5%)
Query: 12 LILVVGVALGVVALVR-TNNGPADANNGGELTSHTKAVT-AVCQNSDDHKFCAD---TLG 66
++L VAL VA V + G + AN L+ + AV + C ++ + C T G
Sbjct: 21 VLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAG 80
Query: 67 SVNTSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALDDCKDLLEFAIDE 126
V + D I+A V +I +V + KL + + K AL DC + ++ +DE
Sbjct: 81 GVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDE 140
Query: 127 LQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFD-TDGEKQVQSQLQTGSL 185
L + + + + A DLK + + Q++CLDGF D +KQV+ L G +
Sbjct: 141 LHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQI 200
Query: 186 DHVGKLTALALDVVTAITKV-LAALDLDLNVKPSSRRLFE----------------VDED 228
HV + + AL ++ +T +A + + ++R+L E +D +
Sbjct: 201 -HVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGELDSE 259
Query: 229 GNPEWMSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVK 288
G P W+S DR+LL G V +A VA DGSG FKTV A+ + P+N RYVI++K
Sbjct: 260 GWPTWLSAGDRRLLQ----GSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIHIK 315
Query: 289 AGVYDEYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMA 348
AGVY E +++ K KKNI+ GDG T+TIITG +N VDG T +AT + V E F+A+ +
Sbjct: 316 AGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDIT 375
Query: 349 FENTAGANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGY 408
F+NTAG +KHQAVALRV D SAF++C + YQDTLY H++RQF+ C I+GTVDFIFG
Sbjct: 376 FQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGN 435
Query: 409 ASTVIQNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSF 468
A+ V+Q+ I R+P + Q+N++ A G N TG+V+Q C I LQ + ++
Sbjct: 436 AAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTY 495
Query: 469 LARPWKAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKWG 528
L RPWK YS+ + M++ I D+I+P+G+ W GT L+T + EY+NTG G+ RVKW
Sbjct: 496 LGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWR 555
Query: 529 --KGVLSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
K + + A+A KYTA Q+I GG WL +TG PF LG
Sbjct: 556 GFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLG 591
>emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]
Length = 579
Score = 380 bits (975), Expect = e-104
Identities = 219/571 (38%), Positives = 323/571 (56%), Gaps = 16/571 (2%)
Query: 2 ERKIFVSGVSLILVVGVALGVVALV--RTNNGPADANNGGELTSHTKAVTAVCQNSDDHK 59
++K+F++ V+ +L+V +GVV V R+NN A+ ++ V + C+N+ +
Sbjct: 14 KKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSAAHAIVKSACENTLHPE 73
Query: 60 FCADTLGSVN-----TSDPNDYIKAVVKTSIESVIKAFNMTDKLAVENEKNNQSTKMALD 114
C T+ SV+ + D I+ + + +V F +KL + K+AL
Sbjct: 74 LCYSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVEKLIKTRKGLKPREKVALH 133
Query: 115 DCKDLLEFAIDELQASSILAADNSSVHNVNDRAADLKNWLGAVFAYQQSCLDGFD-TDGE 173
DC + ++ +DEL + + ++ A DLK + + Q++CLDGF D +
Sbjct: 134 DCLETIDETLDELHTAIKDLELYPNKKSLKAHADDLKTLISSAITNQETCLDGFSHDDAD 193
Query: 174 KQVQSQLQTGSLDHVGKLTALALDVVTAITKVLAALDLDLNVKPSSRRLFEVDEDGNPEW 233
K+V+ L G HV K+ + AL ++ +T A + L ++R+L E D PEW
Sbjct: 194 KKVRKALLKGQ-KHVEKMCSNALAMICNMTDTDIANEQKLKGTTTNRKLRE-DNSEWPEW 251
Query: 234 MSGADRKLLADMSTGMSVTPNAVVAKDGSGKFKTVLDAINSYPKNHQGRYVIYVKAGVYD 293
+S DR+LL + V P+ VVA DGSG FKTV +A+ P+ RYVI +KAGVY
Sbjct: 252 LSAGDRRLLQSST----VRPDVVVAADGSGNFKTVSEAVAKAPEKSSKRYVIRIKAGVYR 307
Query: 294 EYIQIDKTKKNILIYGDGPTKTIITGKKNFVDGVKTIQTATFSTVAEGFIAKAMAFENTA 353
E + + K K NI+ GDG + TIITG +N DG T +AT + V E F+A+ + F+NTA
Sbjct: 308 ENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVGEKFLARDITFQNTA 367
Query: 354 GANKHQAVALRVQGDKSAFFDCAIRGYQDTLYAHAHRQFYRNCEISGTVDFIFGYASTVI 413
GA KHQAVALRV D SAF+ C I YQD+LY H++RQ++ C I+GTVDFIFG A+ V+
Sbjct: 368 GAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGTVDFIFGNAAAVL 427
Query: 414 QNSKIVVRKPEANQQNIIVADGTVQKNMPTGVVLQNCEIMPEPALQPDRLKVRSFLARPW 473
QN I R+P + Q+N++ A G N TG+V+Q C I L+P + ++L RPW
Sbjct: 428 QNCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPW 487
Query: 474 KAYSRAIFMENTIGDLIQPDGFLPWAGTQFLDTCFFAEYANTGPGSNVQARVKWG--KGV 531
K YSR + M+++I D+I G+ W G L+T F+ EY NTG G+ RVKW K +
Sbjct: 488 KEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGTSGRVKWKGFKVI 547
Query: 532 LSKADATKYTAAQWIEGGVWLPATGIPFDLG 562
S +A YT ++I GG WL +TG PF LG
Sbjct: 548 TSATEAQAYTPGRFIAGGSWLSSTGFPFSLG 578
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.317 0.133 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 935,900,195
Number of Sequences: 2540612
Number of extensions: 38814971
Number of successful extensions: 97460
Number of sequences better than 10.0: 392
Number of HSP's better than 10.0 without gapping: 307
Number of HSP's successfully gapped in prelim test: 85
Number of HSP's that attempted gapping in prelim test: 96200
Number of HSP's gapped (non-prelim): 448
length of query: 566
length of database: 863,360,394
effective HSP length: 133
effective length of query: 433
effective length of database: 525,458,998
effective search space: 227523746134
effective search space used: 227523746134
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 78 (34.7 bits)
Medicago: description of AC144728.4