BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144728.11 + phase: 0 /pseudo
(438 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC18922.1| putative reverse transcriptase [Arabidopsis thali... 47 9e-04
gb|AAG51783.1| reverse transcriptase, putative; 16838-20266 [Ara... 47 0.001
gb|AAD24601.1| putative non-LTR retroelement reverse transcripta... 47 0.002
gb|AAD03565.2| putative non-LTR retroelement reverse transcripta... 46 0.003
gb|AAD17398.1| putative non-LTR retroelement reverse transcripta... 46 0.003
pir||T00833 RNA-directed DNA polymerase homolog T13L16.7 - Arabi... 46 0.003
ref|NP_909558.1| putative reverse transcriptase [Oryza sativa] g... 45 0.006
gb|AAC33961.1| contains similarity to reverse trancriptase (Pfam... 44 0.008
ref|NP_174008.1| hypothetical protein [Arabidopsis thaliana] gi|... 44 0.013
dbj|BAA77394.1| SAE1-S9-protein [Brassica rapa] 44 0.013
gb|AAB84340.1| putative non-LTR retroelement reverse transcripta... 42 0.029
gb|AAD21778.1| putative non-LTR retroelement reverse transcripta... 42 0.029
emb|CAE03482.2| OSJNBa0065O17.7 [Oryza sativa (japonica cultivar... 42 0.029
ref|NP_908556.1| OSJNBa0025P13.26 [Oryza sativa (japonica cultiv... 42 0.049
gb|AAM94293.1| putative reverse transcriptase [Sorghum bicolor] 42 0.049
emb|CAB75484.1| putative protein [Arabidopsis thaliana] gi|11357... 41 0.064
ref|NP_174632.1| expressed protein [Arabidopsis thaliana] 41 0.064
ref|NP_197389.1| expressed protein [Arabidopsis thaliana] 41 0.064
gb|AAF18538.1| Very similar to retrotransposon reverse transcrip... 41 0.064
ref|XP_483473.1| cyst nematode resistance protein-like [Oryza sa... 41 0.084
>gb|AAC18922.1| putative reverse transcriptase [Arabidopsis thaliana]
gi|7487819|pir||T00599 probable reverse transcriptase
At2g02650 [imported] - Arabidopsis thaliana
gi|15227011|ref|NP_178368.1| reverse
transcriptase-related [Arabidopsis thaliana]
Length = 365
Score = 47.4 bits (111), Expect = 9e-04
Identities = 19/52 (36%), Positives = 28/52 (53%)
Query: 316 CVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSAGM 367
CV G L T L+ + + C C E E ++H+MF CP++ VW SA +
Sbjct: 53 CVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQSVWRSANI 104
>gb|AAG51783.1| reverse transcriptase, putative; 16838-20266 [Arabidopsis thaliana]
gi|25405244|pir||E96519 probable reverse transcriptase,
16838-20266 [imported] - Arabidopsis thaliana
Length = 1142
Score = 47.0 bits (110), Expect = 0.001
Identities = 19/60 (31%), Positives = 31/60 (51%)
Query: 303 LRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
++ P KL + GC+P L+ +G+ C C SC E +NH +F+C + Q+W
Sbjct: 831 VQCPPKLRHFLWQILSGCVPVSENLRKRGILCDKGCVSCGASEESINHTLFQCHPARQIW 890
>gb|AAD24601.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana] gi|25411729|pir||H84542 hypothetical protein
At2g16680 [imported] - Arabidopsis thaliana
Length = 1319
Score = 46.6 bits (109), Expect = 0.002
Identities = 18/44 (40%), Positives = 25/44 (55%)
Query: 319 GCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
G L L+ +G+ C C E E +NHL+F+CPF+ QVW
Sbjct: 1017 GALAVEDRLRSRGIRTADGCLFCKEEIETINHLLFQCPFARQVW 1060
>gb|AAD03565.2| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana] gi|25411819|pir||H84557 hypothetical protein
At2g17910 [imported] - Arabidopsis thaliana
Length = 1344
Score = 45.8 bits (107), Expect = 0.003
Identities = 17/44 (38%), Positives = 25/44 (56%)
Query: 319 GCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
G +P L+ +G+ C C E E +NH++FECP + QVW
Sbjct: 1043 GAIPVEDRLRTRGIRSDDGCLMCDTENETINHILFECPLARQVW 1086
>gb|AAD17398.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana] gi|25411644|pir||C84530 hypothetical protein
At2g15540 [imported] - Arabidopsis thaliana
Length = 1225
Score = 45.8 bits (107), Expect = 0.003
Identities = 21/60 (35%), Positives = 30/60 (50%)
Query: 303 LRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
+++ RK C+ GCL T L + + C C E E +NHL+F CP S Q+W
Sbjct: 929 IKTTRKFKHFEWQCLSGCLATNQRLFSRHIGTEKVCPRCGAEEESINHLLFLCPPSRQIW 988
>pir||T00833 RNA-directed DNA polymerase homolog T13L16.7 - Arabidopsis thaliana
(fragment)
Length = 1365
Score = 45.8 bits (107), Expect = 0.003
Identities = 17/44 (38%), Positives = 25/44 (56%)
Query: 319 GCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
G +P L+ +G+ C C E E +NH++FECP + QVW
Sbjct: 1064 GAIPVEDRLRTRGIRSDDGCLMCDTENETINHILFECPLARQVW 1107
>ref|NP_909558.1| putative reverse transcriptase [Oryza sativa]
gi|14018103|gb|AAK52166.1| putative reverse
transcriptase [Oryza sativa]
Length = 1185
Score = 44.7 bits (104), Expect = 0.006
Identities = 21/79 (26%), Positives = 37/79 (46%)
Query: 290 TSIVRVIGETFGALRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLN 349
+S R + + L P K+ + G +P + +L DK ++ C CS ED+
Sbjct: 853 SSSSRPVWDNLWKLEIPPKIKIFAWRMLHGVIPCKGILADKHIAVQGGCPVCSSGVEDIK 912
Query: 350 HLMFECPFSIQVWMSAGMW 368
H++F C + +W G+W
Sbjct: 913 HMVFTCRRAKLIWKQLGIW 931
>gb|AAC33961.1| contains similarity to reverse trancriptase (Pfam: rvt.hmm, score:
42.57) [Arabidopsis thaliana] gi|7486711|pir||T01893
hypothetical protein F8M12.22 - Arabidopsis thaliana
Length = 1662
Score = 44.3 bits (103), Expect = 0.008
Identities = 16/46 (34%), Positives = 26/46 (55%)
Query: 317 VGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
+ LPTR L +G+ +C C E E +NH++F CP++ +W
Sbjct: 1379 ISKALPTRSRLLTRGMDIDPHCPRCPTEEETINHVLFTCPYAASIW 1424
>ref|NP_174008.1| hypothetical protein [Arabidopsis thaliana]
gi|4262175|gb|AAD14492.1| 12894 gi|25518121|pir||D86395
T2P11.5 protein - Arabidopsis thaliana
Length = 240
Score = 43.5 bits (101), Expect = 0.013
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 4/84 (4%)
Query: 293 VRVIGETFGALRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLM 352
++V+ ++ P KL + GC+P L+ +G++C C+ C E +NH +
Sbjct: 11 IKVLKAFSWKVQCPPKLYHFLWQILAGCVPITDNLRKRGINCEIGCSRCGALEETINHTL 70
Query: 353 FECPFSIQVW----MSAGMWYMCK 372
F C + Q+W + +WY+ K
Sbjct: 71 FLCHPARQIWALSQIPTIIWYIWK 94
>dbj|BAA77394.1| SAE1-S9-protein [Brassica rapa]
Length = 255
Score = 43.5 bits (101), Expect = 0.013
Identities = 25/91 (27%), Positives = 46/91 (50%), Gaps = 3/91 (3%)
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSAGMWYMCKMRP*IRTL 380
LPTR L G++ P +C C+ E +HL FEC F++ VW+ M P ++T+
Sbjct: 153 LPTRDRLISWGLTVPNSCVLCNGGVESHSHLFFECSFAVGVWLHFCGRSMTSPPPDLQTV 212
Query: 381 LSTQFSIYSK---TRLWIFNHLLLLFVGVYG 408
++ ++ + + + LL + V ++G
Sbjct: 213 VAYCQQLHGPLMVSTMKVMKLLLQVIVTIFG 243
>gb|AAB84340.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana] gi|7488322|pir||T00814 RNA-directed DNA
polymerase homolog At2g41580 - Arabidopsis thaliana
Length = 1094
Score = 42.4 bits (98), Expect = 0.029
Identities = 19/60 (31%), Positives = 30/60 (49%)
Query: 303 LRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
L S K+ + G + L+ +GV C+ C + E LNH++F+CP + QVW
Sbjct: 778 LNSAPKIKVFLWKVLKGAVAVEDRLRTRGVLIEDGCSMCPEKNETLNHILFQCPLARQVW 837
>gb|AAD21778.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana] gi|25410938|pir||G84429 hypothetical protein
At2g01840 [imported] - Arabidopsis thaliana
Length = 1715
Score = 42.4 bits (98), Expect = 0.029
Identities = 16/50 (32%), Positives = 27/50 (54%)
Query: 316 CVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSA 365
C+ G L T L+++ + C C + E +NH++F C ++ VW SA
Sbjct: 1404 CLSGALSTTTQLRNRNIPADPTCQRCCNADETINHIIFTCSYAQVVWRSA 1453
>emb|CAE03482.2| OSJNBa0065O17.7 [Oryza sativa (japonica cultivar-group)]
gi|50927885|ref|XP_473470.1| OSJNBa0065O17.7 [Oryza
sativa (japonica cultivar-group)]
Length = 3331
Score = 42.4 bits (98), Expect = 0.029
Identities = 23/68 (33%), Positives = 34/68 (49%), Gaps = 13/68 (19%)
Query: 296 IGETFGALRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFEC 355
+G+ GAL PR C T LLQ +G+ + C C+ E E NH++ +C
Sbjct: 1530 VGDIRGALTVPR------------CW-TADLLQKRGIDSHSACPFCAQELETANHILIDC 1576
Query: 356 PFSIQVWM 363
F+ QVW+
Sbjct: 1577 VFARQVWL 1584
>ref|NP_908556.1| OSJNBa0025P13.26 [Oryza sativa (japonica cultivar-group)]
Length = 297
Score = 41.6 bits (96), Expect = 0.049
Identities = 16/41 (39%), Positives = 25/41 (60%)
Query: 323 TRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWM 363
T LLQ +G+ + C C+ E E NH++ +C F+ QVW+
Sbjct: 230 TADLLQKRGIDSHSACPFCAQELETANHILIDCVFARQVWL 270
>gb|AAM94293.1| putative reverse transcriptase [Sorghum bicolor]
Length = 1323
Score = 41.6 bits (96), Expect = 0.049
Identities = 15/34 (44%), Positives = 18/34 (52%)
Query: 338 CASCSHEYEDLNHLMFECPFSIQVWMSAGMWYMC 371
CA C E +HL FECP+S VW + W C
Sbjct: 1201 CALCERNLETAHHLFFECPYSRLVWQAVSSWSSC 1234
>emb|CAB75484.1| putative protein [Arabidopsis thaliana] gi|11357921|pir||T47495
hypothetical protein F9K21.130 - Arabidopsis thaliana
Length = 851
Score = 41.2 bits (95), Expect = 0.064
Identities = 16/42 (38%), Positives = 21/42 (49%)
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
LPT L +G+ C C E E +NH +F CPF+ W
Sbjct: 688 LPTTDRLTTRGMRIDPGCPRCRRENESINHALFTCPFATMAW 729
>ref|NP_174632.1| expressed protein [Arabidopsis thaliana]
Length = 210
Score = 41.2 bits (95), Expect = 0.064
Identities = 33/135 (24%), Positives = 60/135 (44%), Gaps = 26/135 (19%)
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSAGM------------- 367
LPT+ L G+ T C CS + ED +HL C F+ +W + +
Sbjct: 73 LPTKTRLASWGMQLQTTCGLCSLDIEDRDHLFLTCEFACFLWHTVSVRLELPAFSFVVWN 132
Query: 368 ----WYMCKMR---P*IRTLL--STQFSIYSKTRLWIFNHLLLL----FVGVYGSIEISK 414
W + + R P +R L+ S ++I+ + ++ NH +L F + I+ S
Sbjct: 133 DLMDWTLQRNRRSPPTLRKLIVQSVLYAIWKQRNNFLHNHETILPSVVFKTIDREIKNSI 192
Query: 415 FGKR*RRIARKLLTV 429
+R ++ R+L+T+
Sbjct: 193 TARRLKKRFRRLMTL 207
>ref|NP_197389.1| expressed protein [Arabidopsis thaliana]
Length = 295
Score = 41.2 bits (95), Expect = 0.064
Identities = 21/55 (38%), Positives = 31/55 (56%), Gaps = 4/55 (7%)
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSAGMWYMCKMRP 375
LPTR L+ G++ P++ CS+ E HL FEC FS+ +W ++ K RP
Sbjct: 152 LPTRDRLRGWGMNIPSSWVLCSNGDETHAHLFFECSFSLAIW----EFFASKFRP 202
>gb|AAF18538.1| Very similar to retrotransposon reverse transcriptase [Arabidopsis
thaliana] gi|25518314|pir||A86359 hypothetical protein
F12K8.9 - Arabidopsis thaliana
Length = 1231
Score = 41.2 bits (95), Expect = 0.064
Identities = 18/60 (30%), Positives = 30/60 (50%)
Query: 303 LRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
L++ K+ + G +P LL +G+ + C +C E E + H++F C F QVW
Sbjct: 897 LQTEPKIKVFLWKVLSGAIPVVDLLSYRGMKLDSRCQTCGCEGESIQHVLFSCSFPRQVW 956
>ref|XP_483473.1| cyst nematode resistance protein-like [Oryza sativa (japonica
cultivar-group)] gi|42407982|dbj|BAD09120.1| cyst
nematode resistance protein-like [Oryza sativa (japonica
cultivar-group)] gi|42407880|dbj|BAD09021.1| cyst
nematode resistance protein-like [Oryza sativa (japonica
cultivar-group)]
Length = 158
Score = 40.8 bits (94), Expect = 0.084
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Query: 304 RSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW- 362
R+P ++ + G T LQ +G +C C+ E E NHL+ +CP + +VW
Sbjct: 51 RAPSRIKFFMWLATKGRCLTADNLQKRGWPHDASCGLCNAEMESCNHLLMKCPLTNRVWR 110
Query: 363 MSAGMWYMCKMR 374
M W++ K R
Sbjct: 111 MLMVCWFIWKER 122
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.359 0.163 0.562
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 586,004,763
Number of Sequences: 2540612
Number of extensions: 20061414
Number of successful extensions: 80188
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 79
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 80100
Number of HSP's gapped (non-prelim): 93
length of query: 438
length of database: 863,360,394
effective HSP length: 131
effective length of query: 307
effective length of database: 530,540,222
effective search space: 162875848154
effective search space used: 162875848154
T: 11
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 77 (34.3 bits)
Medicago: description of AC144728.11