BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144656.5 + phase: 0 /pseudo
(2301 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_703525.1| hypothetical protein [Plasmodium falciparum 3D7... 44 0.050
ref|XP_640679.1| hypothetical protein DDB0205584 [Dictyostelium ... 44 0.085
ref|XP_646194.1| hypothetical protein DDB0216684 [Dictyostelium ... 42 0.19
ref|NP_702067.1| hypothetical protein PF14_0179 [Plasmodium falc... 42 0.25
ref|NP_701837.1| hypothetical protein PFL2385c [Plasmodium falci... 42 0.25
ref|XP_646224.1| ABC transporter B family protein [Dictyostelium... 42 0.25
ref|XP_639282.1| putative tyrosine kinase-like protein [Dictyost... 42 0.32
ref|NP_701028.1| hypothetical protein PF11_0168 [Plasmodium falc... 41 0.42
gb|AAO53092.1| similar to Dictyostelium discoideum (Slime mold).... 41 0.42
emb|CAE46396.1| Dd-STATb protein [Dictyostelium discoideum] gi|6... 40 0.72
ref|NP_473166.2| hypothetical protein [Plasmodium falciparum 3D7... 40 0.94
ref|XP_647318.1| hypothetical protein DDB0189556 [Dictyostelium ... 40 0.94
ref|XP_647249.1| hypothetical protein DDB0189487 [Dictyostelium ... 40 0.94
ref|XP_645854.1| RasGEF domain-containing protein [Dictyostelium... 40 0.94
gb|AAK54089.2| histidine kinase DhkH [Dictyostelium discoideum] 40 1.2
ref|XP_647674.1| hypothetical protein DDB0189893 [Dictyostelium ... 40 1.2
ref|XP_641414.1| histidine kinase [Dictyostelium discoideum] gi|... 40 1.2
ref|XP_636830.1| hypothetical protein DDB0219317 [Dictyostelium ... 40 1.2
gb|EAA22581.1| hypothetical protein [Plasmodium yoelii yoelii] 40 1.2
ref|XP_646560.1| hypothetical protein DDB0201959 [Dictyostelium ... 39 1.6
>ref|NP_703525.1| hypothetical protein [Plasmodium falciparum 3D7]
gi|23504667|emb|CAD51545.1| hypothetical protein
[Plasmodium falciparum 3D7]
Length = 1379
Score = 44.3 bits (103), Expect = 0.050
Identities = 22/68 (32%), Positives = 36/68 (52%), Gaps = 6/68 (8%)
Query: 384 NKLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNS 443
N +I T + +H ++ +S NI+N + NN+ N H +N HINNN INNN
Sbjct: 1023 NNIIDDTMYNEKNH------DNNNMSCQDMNIVNYNIKNNLNNNHINNNHINNNDINNNH 1076
Query: 444 LDHKKCKL 451
+++ +
Sbjct: 1077 INNNNLNI 1084
>ref|XP_640679.1| hypothetical protein DDB0205584 [Dictyostelium discoideum]
gi|60468700|gb|EAL66702.1| hypothetical protein
DDB0205584 [Dictyostelium discoideum]
Length = 1655
Score = 43.5 bits (101), Expect = 0.085
Identities = 23/65 (35%), Positives = 36/65 (55%)
Query: 394 LPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSLDHKKCKLTQ 453
LP+HL P+ I SL+ +LN NNN N + +N NN+ NNN+ ++ + +
Sbjct: 1507 LPTHLSPLQQISNITSLNDSTLLNNNNNNNNNNNNNNNNQSNNSNNNNNNKNNNNNRFSS 1566
Query: 454 SLLLT 458
S LL+
Sbjct: 1567 SKLLS 1571
>ref|XP_646194.1| hypothetical protein DDB0216684 [Dictyostelium discoideum]
gi|60474872|gb|EAL72809.1| hypothetical protein
DDB0216684 [Dictyostelium discoideum]
Length = 1342
Score = 42.4 bits (98), Expect = 0.19
Identities = 22/49 (44%), Positives = 25/49 (50%)
Query: 395 PSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNS 443
P H LPM +H+I + N N NNN N SN NNN NNNS
Sbjct: 595 PQHPLPMSVSHEIHNFKFKNSTNSNNNNNNNNNSNSNTTTNNNTNNNNS 643
>ref|NP_702067.1| hypothetical protein PF14_0179 [Plasmodium falciparum 3D7]
gi|23497245|gb|AAN36791.1| hypothetical protein
[Plasmodium falciparum 3D7]
Length = 3597
Score = 42.0 bits (97), Expect = 0.25
Identities = 24/64 (37%), Positives = 37/64 (57%), Gaps = 6/64 (9%)
Query: 399 LPMLFNHQIVSLDSHNILNK------ILNNNIPNQHTSNAHINNNPINNNSLDHKKCKLT 452
L +L N+ I ++ ++N+ N I NNNI N + +N +INNN INNN+ K K
Sbjct: 2391 LNILINNDINNMHNNNVNNNYVNNNNINNNNINNNNINNNNINNNNINNNNFSFGKKKFV 2450
Query: 453 QSLL 456
++L
Sbjct: 2451 ITVL 2454
>ref|NP_701837.1| hypothetical protein PFL2385c [Plasmodium falciparum 3D7]
gi|23497011|gb|AAN36561.1| hypothetical protein PFL2385c
[Plasmodium falciparum 3D7]
Length = 788
Score = 42.0 bits (97), Expect = 0.25
Identities = 26/70 (37%), Positives = 40/70 (57%), Gaps = 13/70 (18%)
Query: 383 PNKLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNN------IPNQHTSNAHINN 436
PN + NI +H+ N+ I LD+++I N IL+NN + N H +N H+NN
Sbjct: 161 PNNNFVNNNILDNNHI-----NNNI--LDNNHINNNILDNNHINNNHLNNNHLNNNHLNN 213
Query: 437 NPINNNSLDH 446
N +NNN L++
Sbjct: 214 NHLNNNHLNN 223
Score = 39.3 bits (90), Expect = 1.6
Identities = 17/39 (43%), Positives = 29/39 (73%), Gaps = 1/39 (2%)
Query: 410 LDSHNILNKILNNN-IPNQHTSNAHINNNPINNNSLDHK 447
L+++++ N LNNN + N H +N H+NNN +NN+ LD++
Sbjct: 206 LNNNHLNNNHLNNNHLNNNHFNNNHLNNNQLNNHLLDNR 244
>ref|XP_646224.1| ABC transporter B family protein [Dictyostelium discoideum]
gi|60474273|gb|EAL72210.1| ABC transporter B family
protein [Dictyostelium discoideum]
Length = 824
Score = 42.0 bits (97), Expect = 0.25
Identities = 33/95 (34%), Positives = 47/95 (48%), Gaps = 6/95 (6%)
Query: 381 EEPNKLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPIN 440
E NK S +L + L P++ N S D++N N NNN N + +N + NNN N
Sbjct: 35 ENKNKKRNSVKFNLDTPL-PIINNKS--SQDNNNKNN---NNNNNNNNNNNNNNNNNNNN 88
Query: 441 NNSLDHKKCKLTQSLLLTQSYSQDYSERI*FKPSL 475
NN+ + K K+ SL Y DY + FK S+
Sbjct: 89 NNNNKNNKNKINNSLNSITPYLVDYKDDFDFKRSV 123
>ref|XP_639282.1| putative tyrosine kinase-like protein [Dictyostelium discoideum]
gi|60467912|gb|EAL65925.1| putative tyrosine kinase-like
(TKL) protein [Dictyostelium discoideum]
Length = 1761
Score = 41.6 bits (96), Expect = 0.32
Identities = 22/62 (35%), Positives = 33/62 (52%)
Query: 397 HLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSLDHKKCKLTQSLL 456
HL P+ + + S S + LN NN PN + SN++ NNN NNN+ ++ K T +
Sbjct: 1388 HLTPLSNSTSLSSASSISFLNSSNGNNSPNSNNSNSNNNNNNNNNNNNNNNNKKTTTTTT 1447
Query: 457 LT 458
T
Sbjct: 1448 TT 1449
>ref|NP_701028.1| hypothetical protein PF11_0168 [Plasmodium falciparum 3D7]
gi|23496091|gb|AAN35752.1| hypothetical protein
[Plasmodium falciparum 3D7]
Length = 2966
Score = 41.2 bits (95), Expect = 0.42
Identities = 32/90 (35%), Positives = 41/90 (45%), Gaps = 9/90 (10%)
Query: 411 DSHNILNKILNNNI-PNQHTSNAHINNNPINNNSLDHKKCKLTQSLLLTQSYSQDYSERI 469
D NI LN+N+ N H + HI N I N S DHKK K S ++ + S+ YSE
Sbjct: 476 DKENINENPLNDNVYKNYHHFDQHILNYNIYNTSNDHKKNKAVPSHIINRFRSERYSENS 535
Query: 470 *FKPSLLLQYQ--------KECQHGTDLIR 491
K YQ K C+ T L+R
Sbjct: 536 TMKYEGKNYYQSFMSQSALKRCRSYTILLR 565
>gb|AAO53092.1| similar to Dictyostelium discoideum (Slime mold). Nucleotide
exchange factor RasGEF N
Length = 1672
Score = 41.2 bits (95), Expect = 0.42
Identities = 25/102 (24%), Positives = 54/102 (52%), Gaps = 4/102 (3%)
Query: 343 KESEKDV**KMELLLVEPRNLGTTSQERKNTMLMW*PMEEPNKLIQSTNISLPSHLLPML 402
K+++ + + + PRN+ ++ +T+ P++ P +I S+ + S + P+
Sbjct: 13 KDAKNKIEEPQQSTIASPRNICIST----STLSPPPPLQLPPTVIPSSTSTTNSMISPLY 68
Query: 403 FNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSL 444
N +++ +++N N NNN N + +N + NNN NNN++
Sbjct: 69 TNSSLINNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNI 110
>emb|CAE46396.1| Dd-STATb protein [Dictyostelium discoideum]
gi|66826959|ref|XP_646834.1| STAT [Dictyostelium
discoideum] gi|60474972|gb|EAL72908.1| signal transducer
and activator of transcription (STAT) family protein
[Dictyostelium discoideum]
Length = 1148
Score = 40.4 bits (93), Expect = 0.72
Identities = 20/64 (31%), Positives = 35/64 (54%)
Query: 380 MEEPNKLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPI 439
+E+ N +++ N+ L L M + + + + N N +NNN N + +N + NNN I
Sbjct: 233 VEDINAKLENENLQLKKELFEMSRKFKEIDIINLNNTNNNINNNNNNNNNNNNNNNNNSI 292
Query: 440 NNNS 443
NNN+
Sbjct: 293 NNNN 296
>ref|NP_473166.2| hypothetical protein [Plasmodium falciparum 3D7]
gi|23477022|emb|CAB38989.3| hypothetical protein,
conserved [Plasmodium falciparum 3D7]
Length = 2226
Score = 40.0 bits (92), Expect = 0.94
Identities = 30/82 (36%), Positives = 44/82 (53%), Gaps = 8/82 (9%)
Query: 385 KLIQSTNISLPSHLLPMLF--NHQIVSLDSH-NILNKILNNNIP--NQHTSNAHINNNPI 439
KLI ++I H+L F N QI ++ +H N+ + +LN N N H N + NNN
Sbjct: 1771 KLISISHI-FTRHILKFNFDKNEQIENITNHGNVKSNVLNPNTTHINNHPHNQYHNNNKY 1829
Query: 440 NNNSLDHKKCKLTQSLLLTQSY 461
NN + HK K+ Q LL ++Y
Sbjct: 1830 NNKNNYHK--KIIQELLRDKAY 1849
>ref|XP_647318.1| hypothetical protein DDB0189556 [Dictyostelium discoideum]
gi|60475417|gb|EAL73352.1| hypothetical protein
DDB0189556 [Dictyostelium discoideum]
Length = 1055
Score = 40.0 bits (92), Expect = 0.94
Identities = 17/40 (42%), Positives = 28/40 (69%)
Query: 404 NHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNS 443
N+ I++++++NI N NNN N + SN++ NNN NNN+
Sbjct: 127 NNNIININNNNINNNNSNNNYSNNNNSNSNNNNNNNNNNN 166
>ref|XP_647249.1| hypothetical protein DDB0189487 [Dictyostelium discoideum]
gi|60475376|gb|EAL73311.1| hypothetical protein
DDB0189487 [Dictyostelium discoideum]
Length = 509
Score = 40.0 bits (92), Expect = 0.94
Identities = 25/76 (32%), Positives = 40/76 (51%), Gaps = 4/76 (5%)
Query: 411 DSHNILNKILNNNIPNQHTSNAHINNNPINNNSLDHKKCKLTQSLLLTQSYSQDYSERI* 470
+++N N NNN N + +N +INNN INNN+ D + L+ +Y Y +
Sbjct: 290 NNNNNNNNNNNNNNNNNNNNNNNINNNYINNNNNDSWNNQFQNQLMYCNNYQAQYQQ--- 346
Query: 471 FKPS-LLLQYQKECQH 485
P+ LQYQ++ Q+
Sbjct: 347 LHPNHYQLQYQQQQQN 362
>ref|XP_645854.1| RasGEF domain-containing protein [Dictyostelium discoideum]
gi|60474062|gb|EAL71999.1| putative tyrosine kinase-like
(TKL) protein [Dictyostelium discoideum]
Length = 960
Score = 40.0 bits (92), Expect = 0.94
Identities = 32/114 (28%), Positives = 58/114 (50%), Gaps = 4/114 (3%)
Query: 356 LLVEPRNLGTTSQERKNTMLMW*PMEEPNKLIQSTNISL--PSHLLPMLFNHQIVSLDSH 413
LL++P+ L T Q++++ + + + P+ I + N + P + + + N+ V+ S
Sbjct: 565 LLIDPK-LMTELQKKRSELELLIQINSPSDFINNNNNNNNNPVNNINNINNNNSVNSSSS 623
Query: 414 NILNKILNNNIPNQHTSNAHINNNPINNNSLD-HKKCKLTQSLLLTQSYSQDYS 466
N N NNN N + +N + NNN NNN L+ QS ++ Q+ + YS
Sbjct: 624 NNNNNNNNNNSNNNNNNNNNNNNNNNNNNGLNIINIAAANQSKMMLQNGNNRYS 677
>gb|AAK54089.2| histidine kinase DhkH [Dictyostelium discoideum]
Length = 1147
Score = 39.7 bits (91), Expect = 1.2
Identities = 31/102 (30%), Positives = 47/102 (45%), Gaps = 6/102 (5%)
Query: 385 KLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSL 444
K ++ +N H P L + + SLD +N N NNNI N + N + NNN NNN+
Sbjct: 891 KKLKKSNSDQSIHFSPSLTSSSLPSLDLNNNNNINNNNNINNNNNINNNNNNNNNNNNNN 950
Query: 445 DHKKCKL----TQSLLLTQSYSQDYSERI*FKPSLLLQYQKE 482
++ L + S+L + Q + LL YQK+
Sbjct: 951 NNNNNNLNHYNSDSILSSDLSPQQHQYH--HPNPLLANYQKK 990
>ref|XP_647674.1| hypothetical protein DDB0189893 [Dictyostelium discoideum]
gi|60475641|gb|EAL73576.1| hypothetical protein
DDB0189893 [Dictyostelium discoideum]
Length = 825
Score = 39.7 bits (91), Expect = 1.2
Identities = 32/117 (27%), Positives = 52/117 (44%), Gaps = 15/117 (12%)
Query: 388 QSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSLDHK 447
Q I + L + N+ L++++I+N NN N + +N NNN NNN + +
Sbjct: 126 QENRIQFVFNQLQSIINNNNYKLNNYSIINNSNLNNSNNNNNNNNSNNNNNNNNNEYNIR 185
Query: 448 K--------CKLTQSLLLTQSYSQDYSERI*F---KPSLLLQYQ----KECQHGTDL 489
K CK + L + Y+ + I F K + L+YQ K+ QH D+
Sbjct: 186 KSIENLTEWCKYSNQLYEIEKYNNVILKDIIFNKSKENWELEYQTINNKQAQHYLDI 242
>ref|XP_641414.1| histidine kinase [Dictyostelium discoideum]
gi|60469435|gb|EAL67428.1| histidine kinase
[Dictyostelium discoideum]
Length = 1378
Score = 39.7 bits (91), Expect = 1.2
Identities = 31/102 (30%), Positives = 47/102 (45%), Gaps = 6/102 (5%)
Query: 385 KLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSL 444
K ++ +N H P L + + SLD +N N NNNI N + N + NNN NNN+
Sbjct: 1122 KKLKKSNSDQSIHFSPSLTSSSLPSLDLNNNNNINNNNNINNNNNINNNNNNNNNNNNNN 1181
Query: 445 DHKKCKL----TQSLLLTQSYSQDYSERI*FKPSLLLQYQKE 482
++ L + S+L + Q + LL YQK+
Sbjct: 1182 NNNNNNLNHYNSDSILSSDLSPQQHQYH--HPNPLLANYQKK 1221
>ref|XP_636830.1| hypothetical protein DDB0219317 [Dictyostelium discoideum]
gi|60465255|gb|EAL63349.1| hypothetical protein
DDB0219317 [Dictyostelium discoideum]
Length = 1523
Score = 39.7 bits (91), Expect = 1.2
Identities = 21/52 (40%), Positives = 31/52 (59%)
Query: 411 DSHNILNKILNNNIPNQHTSNAHINNNPINNNSLDHKKCKLTQSLLLTQSYS 462
+++NI N I NNN N +++N +INNN NNNS + + +L QS S
Sbjct: 845 NNNNINNNIANNNNNNNNSNNNNINNNINNNNSFNSSASVDRKQILSNQSES 896
Score = 37.0 bits (84), Expect = 8.0
Identities = 26/74 (35%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Query: 404 NHQIVSLDSHNILNKILNNNIPNQHTSNAHINNNPINNNSLDHKKCKLTQSLLLTQSYSQ 463
N+ VS +++ N +NNNI N + +N + NNN INNN ++ + S+ Q S
Sbjct: 833 NNNNVSNNNNINNNNNINNNIANNNNNNNNSNNNNINNNINNNNSFNSSASVDRKQILS- 891
Query: 464 DYSERI*FKPSLLL 477
+ SE + PSL+L
Sbjct: 892 NQSESLSKLPSLVL 905
>gb|EAA22581.1| hypothetical protein [Plasmodium yoelii yoelii]
Length = 2522
Score = 39.7 bits (91), Expect = 1.2
Identities = 27/87 (31%), Positives = 49/87 (56%), Gaps = 13/87 (14%)
Query: 391 NISLPSHLLPMLFNHQIVSLDSH------NILNKILNNNIPNQHTSNAHINNNP---INN 441
NI++ ++ P ++QI+ L+++ N +N I NNNIP ++T N + N++P INN
Sbjct: 917 NINM-NNFSPYKKDNQIIQLNNYSKYEYNNFINNIKNNNIPYENTINNNFNDSPIDIINN 975
Query: 442 NSLDHKKCKLTQSL---LLTQSYSQDY 465
N L + Q++ +LT + +Y
Sbjct: 976 NHLYNISINSNQNVNNSILTDGHINNY 1002
>ref|XP_646560.1| hypothetical protein DDB0201959 [Dictyostelium discoideum]
gi|60474780|gb|EAL72717.1| hypothetical protein
DDB0201959 [Dictyostelium discoideum]
Length = 578
Score = 39.3 bits (90), Expect = 1.6
Identities = 30/102 (29%), Positives = 47/102 (45%), Gaps = 10/102 (9%)
Query: 368 QERKNTMLMW*PMEEPNKLIQSTNISLPSHLLPMLFNHQIVSLDSHNILNKIL------- 420
+E K W +EP K I I + L P L + +I++ + N +K L
Sbjct: 314 EEEKEEEEEWMSDDEPYKSIDPNVIEKTNQLFPSLISSKILNT-TKNSKSKSLKSQLSIP 372
Query: 421 --NNNIPNQHTSNAHINNNPINNNSLDHKKCKLTQSLLLTQS 460
NNN N + +N + NNN NNN+ D+ K Q+ +T +
Sbjct: 373 NNNNNNNNNNNNNNNNNNNNNNNNNNDNNNNKQQQNTNITNT 414
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.358 0.158 0.577
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,259,882,721
Number of Sequences: 2540612
Number of extensions: 118251174
Number of successful extensions: 660378
Number of sequences better than 10.0: 90
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 66
Number of HSP's that attempted gapping in prelim test: 651873
Number of HSP's gapped (non-prelim): 2842
length of query: 2301
length of database: 863,360,394
effective HSP length: 144
effective length of query: 2157
effective length of database: 497,512,266
effective search space: 1073133957762
effective search space used: 1073133957762
T: 11
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 84 (37.0 bits)
Medicago: description of AC144656.5