BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144563.4 + phase: 0
(588 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultiva... 404 e-111
gb|AAC34241.1| putative pectinesterase [Arabidopsis thaliana] gi... 382 e-104
gb|AAC27719.1| flower-specific pectin methylesterase precursor [... 380 e-104
gb|AAV91509.1| VGD1-like protein 1 [Arabidopsis thaliana] 379 e-103
emb|CAA73733.1| pectin methylesterase-like protein [Zea mays] gi... 377 e-103
pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilg... 370 e-101
emb|CAA39658.1| Bp19 [Brassica napus] gi|99823|pir||S14952 pecti... 351 3e-95
emb|CAB71877.1| PECTINESTERASE-like protein [Arabidopsis thalian... 350 9e-95
gb|AAV91510.1| VGD1-like protein 2 [Arabidopsis thaliana] 345 2e-93
ref|XP_474065.1| OSJNBb0079B02.7 [Oryza sativa (japonica cultiva... 333 1e-89
emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (in... 333 1e-89
ref|NP_198139.1| pectinesterase family protein [Arabidopsis thal... 333 1e-89
emb|CAB65290.2| pectin methyl-esterase PER [Medicago truncatula] 330 6e-89
dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica ... 330 6e-89
pir||T52325 pectinesterase (EC 3.1.1.11) [imported] - turnip (fr... 330 6e-89
ref|XP_479611.1| putative pectin methylesterase [Oryza sativa (j... 329 2e-88
gb|AAF26136.1| putative pectinesterase [Arabidopsis thaliana] gi... 327 7e-88
gb|AAB57670.1| pectinesterase [Citrus sinensis] 327 9e-88
ref|NP_850077.1| pectinesterase family protein [Arabidopsis thal... 326 1e-87
sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methyles... 326 1e-87
>emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultivar-group)]
gi|50924778|ref|XP_472740.1| OSJNBa0036B21.20 [Oryza
sativa (japonica cultivar-group)]
Length = 568
Score = 404 bits (1038), Expect = e-111
Identities = 237/599 (39%), Positives = 342/599 (56%), Gaps = 44/599 (7%)
Query: 1 MKGKVIISVVSLILVVGVIIGVVVDIRKNGE----------DPKVQTQQRNLRIMCQNAQ 50
M K ++ V ILVV V++GVV + ++G + + T +++ +C
Sbjct: 1 MAQKFLLGGVGAILVVAVVVGVVATVTRSGNKAGDNFTVPGEANLATSGKSVESLCAPTL 60
Query: 51 DQKLCHDTLSSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALD 110
++ C TL++ +PK + K+A +++ A S+ + + + A +
Sbjct: 61 YKESCEKTLTTATSG-TENPKEVFSTVAKSALESIKSAVEKSKAIGEAKTSDSMTESARE 119
Query: 111 DCKDLMQFALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGE 170
DCK L++ ++D L + +++ + ++ D+ +WL+ V+++ C +GF D
Sbjct: 120 DCKALLEDSVDDLRGMVE-MAGGDVKVLFSRSDDLEHWLTGVMTFMDTCADGFAD----- 173
Query: 171 KKIKEQFHVQSLYSVQKVTAVALDIVTGLSDILQQFNLNF--DIKPPSRRLLNSEVTVDD 228
+K+K H L + ++++ AL I L I ++ +L+ P R L+ + TV
Sbjct: 174 EKLKADMH-SVLRNASELSSNALAITNTLGAIFKKLDLDMFKGENPIHRSLIAEQETVG- 231
Query: 229 QGYPSWISSSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYV 288
G+PSW+ + RKLLA G R +PNAVVA DGSGQFKTIQ A+ S PKG++ RYV
Sbjct: 232 -GFPSWMKAPDRKLLAS----GDRNRPQPNAVVAQDGSGQFKTIQEAVNSMPKGHQGRYV 286
Query: 289 IYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIG 348
IYVKAG+YDE + VPK+ VNI MYGDGP ++ VTGRK+ G T TATFS A GFI
Sbjct: 287 IYVKAGLYDEIVMVPKDKVNIFMYGDGPKRSRVTGRKSFADGITTMKTATFSVEAAGFIC 346
Query: 349 KAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDF 408
K M F NTAG QAVA R GD+ A C +QDTLYV RQF+RNCVISGTIDF
Sbjct: 347 KNMGFHNTAGAERHQAVALRINGDLGAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDF 406
Query: 409 IFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEK 468
IFG SA + Q+ II R+ N+ N + A G NM +G+VIQ+C ++P+ L P++
Sbjct: 407 IFGNSAAVFQNCLIITRRPM--DNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDR 464
Query: 469 FTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTG 528
F + SYLGRPW+ S+ VIMESTI DFI +G+ W E NT Y+AE+ N G
Sbjct: 465 FKIPSYLGRPWKEYSRLVIMESTIADFIKPEGYMPWNGE------FALNTLYYAEFNNRG 518
Query: 529 PGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHYLGFK 587
PGA ++RV WKG++ VI + EA ++TA ++D G WL PH+LGFK
Sbjct: 519 PGAGTSKRVNWKGFR-VIGQKEAEQFTAGPFVDGG---------TWLKFTGTPHFLGFK 567
>gb|AAC34241.1| putative pectinesterase [Arabidopsis thaliana]
gi|15450765|gb|AAK96654.1| putative pectinesterase
[Arabidopsis thaliana] gi|61213847|sp|O80722|PME4_ARATH
Pectinesterase-4 precursor (Pectin methylesterase 4) (PE
4) (VANGUARD1-like protein 1) (VGD1-like protein 1)
(AtPME4) gi|15226482|ref|NP_182226.1| pectinesterase
family protein [Arabidopsis thaliana]
Length = 588
Score = 382 bits (982), Expect = e-104
Identities = 231/620 (37%), Positives = 339/620 (54%), Gaps = 68/620 (10%)
Query: 1 MKGKVIISVVSLILVVGVIIGVVVDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTLS 60
M GKV++SV S++L+VGV IGVV I KNG D + Q + ++ +CQ+ D+ C TL
Sbjct: 1 MIGKVVVSVASILLIVGVAIGVVAFINKNG-DANLSPQMKAVQGICQSTSDKASCVKTLE 59
Query: 61 SVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGK--KNGAKMALDDCKDLMQF 118
V+ + DP I A + A D + K+ N + + G K LD CK + +
Sbjct: 60 PVK---SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 119 ALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFH 178
AL+ L + + ++ + + ++ WL V +Y+ C++ ++ +D K I E
Sbjct: 117 ALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE-DDLRKAIGE--- 171
Query: 179 VQSLYSVQKVTAVALDIVTGLSDILQQFNLNF-------------------------DIK 213
+ + + +T A+DI + + + N D
Sbjct: 172 --GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 229
Query: 214 PPSRRLLNSEVTVDDQGYPSWISSSGRKLLAKMQR-----KGWRANIRPNAVVANDGSGQ 268
P+RRLL +D+ G P+W+S + RKL+AK R +G A +R N VVA DGSGQ
Sbjct: 230 GPARRLLED---IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 286
Query: 269 FKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKN-- 326
FKT+Q A+ + P+ N+ R +IY+KAG+Y E + +PK+ NI M+GDG KT+++ ++
Sbjct: 287 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 346
Query: 327 QMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQD 386
GT T +AT + GF+ K M F+NTAGP G QA A R GD + + C GYQD
Sbjct: 347 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 406
Query: 387 TLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPL-V 445
TLYV RQFYRNCV+SGT+DFIFG SAT+IQ++ I+VRKG+ +YN + ADG+ L +
Sbjct: 407 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGS--KGQYNTVTADGNELGL 464
Query: 446 NMNTGIVIQDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWP 505
M GIV+Q+C I+P+ L PE+ TV +YLGRPW+ S VIM + +GD I +GW W
Sbjct: 465 GMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWD 524
Query: 506 EEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPK 565
E +C + EY N GPGA RRV W K S +E +TA+ WL GP
Sbjct: 525 GES------FHKSCRYVEYNNRGPGAFANRRVNWA--KVARSAAEVNGFTAANWL--GP- 573
Query: 566 TAPKSAVEWLHDLHVPHYLG 585
+ W+ + +VP +G
Sbjct: 574 ------INWIQEANVPVTIG 587
>gb|AAC27719.1| flower-specific pectin methylesterase precursor [Arabidopsis
thaliana]
Length = 586
Score = 380 bits (976), Expect = e-104
Identities = 227/618 (36%), Positives = 337/618 (53%), Gaps = 66/618 (10%)
Query: 1 MKGKVIISVVSLILVVGVIIGVVVDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTLS 60
M GKV++SV S++L+VGV IGVV I KNG D + Q + ++ +CQ+ D+ C TL
Sbjct: 1 MIGKVVVSVASILLIVGVAIGVVAFINKNG-DANLSPQMKAVQGICQSTSDKASCVKTLE 59
Query: 61 SVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGK--KNGAKMALDDCKDLMQF 118
V+ + DP I A + A D + K+ N + + G K LD CK + +
Sbjct: 60 PVK---SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 119 ALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFH 178
AL+ L + + ++ + + ++ WL V +Y+ C++ ++ +D K I E
Sbjct: 117 ALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE-DDLRKAIGE--- 171
Query: 179 VQSLYSVQKVTAVALDIVTGLSDILQQFNLNF-------------------------DIK 213
+ + + +T A+DI + + + N D
Sbjct: 172 --GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 229
Query: 214 PPSRRLLNSEVTVDDQGYPSWISSSGRKLLAKMQR-----KGWRANIRPNAVVANDGSGQ 268
P+RRLL +D+ G P+W+S + RKL+AK R +G A +R N VVA DGSGQ
Sbjct: 230 GPARRLLED---IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 286
Query: 269 FKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQM 328
FKT+Q A+ + P+ N+ R +IY+KAG+Y E + +PK+ NI M+GDG KT+++ ++
Sbjct: 287 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 346
Query: 329 AGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTL 388
T + + + + GF+ K M F+NTAGP G QA A R GD + + C GYQDTL
Sbjct: 347 LSRGTTTSLSATVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTL 406
Query: 389 YVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPL-VNM 447
YV RQFYRNCV+SGT+DFIFG SAT+IQ++ I+VRKG+ +YN + ADG+ L + M
Sbjct: 407 YVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGS--KGQYNTVTADGNELGLGM 464
Query: 448 NTGIVIQDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEE 507
GIV+Q+C I+P+ L PE+ TV +YLGRPW+ S VIM + +GD I +GW W E
Sbjct: 465 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 524
Query: 508 QNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTA 567
+C + EY N GPGA RRV W K S +E +TA+ WL GP
Sbjct: 525 S------FHKSCRYVEYNNRGPGAFANRRVNWA--KVARSAAEVNGFTAANWL--GP--- 571
Query: 568 PKSAVEWLHDLHVPHYLG 585
+ W+ + +VP +G
Sbjct: 572 ----INWIQEANVPVTIG 585
>gb|AAV91509.1| VGD1-like protein 1 [Arabidopsis thaliana]
Length = 588
Score = 379 bits (974), Expect = e-103
Identities = 229/620 (36%), Positives = 339/620 (53%), Gaps = 68/620 (10%)
Query: 1 MKGKVIISVVSLILVVGVIIGVVVDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTLS 60
M GKV++SV S++L+VGV IGVV I KNG D + Q + ++ +C++ D+ C TL
Sbjct: 1 MIGKVVVSVASILLIVGVAIGVVAFINKNG-DANLSPQMKAVQGICRSTSDKASCVKTLE 59
Query: 61 SVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGK--KNGAKMALDDCKDLMQF 118
V+ + DP I A + A D + K+ N + + G K LD CK + +
Sbjct: 60 PVK---SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 119 ALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFH 178
AL+ L + + ++ + + ++ WL V +Y+ C++ ++ +D K I E
Sbjct: 117 ALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE-DDLRKAIGE--- 171
Query: 179 VQSLYSVQKVTAVALDIVTGLSDILQQFNLNF-------------------------DIK 213
+ + + +T A+DI + + + N D
Sbjct: 172 --GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 229
Query: 214 PPSRRLLNSEVTVDDQGYPSWISSSGRKLLAKMQR-----KGWRANIRPNAVVANDGSGQ 268
P+RRLL +D+ G P+W+S + RKL+AK R +G A +R N VVA DGSGQ
Sbjct: 230 GPARRLLED---IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRANFVVAKDGSGQ 286
Query: 269 FKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKN-- 326
FKT+Q A+ + P+ N+ R +IY+KAG+Y E + +PK+ NI M+GDG KT+++ ++
Sbjct: 287 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKNNNIFMFGDGARKTVISYNRSVA 346
Query: 327 QMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQD 386
GT T +AT + GF+ K M F+NTAGP G QA A R GD + + C GYQD
Sbjct: 347 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 406
Query: 387 TLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPL-V 445
TLYV RQFYRNCV+SGT+DFIFG SAT+IQ++ I+VRKG+ +YN + ADG+ L +
Sbjct: 407 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGS--KGQYNTVTADGNELGL 464
Query: 446 NMNTGIVIQDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWP 505
M GIV+Q+C I+P+ L PE+ TV +YLGRPW+ S VIM + +GD I +GW W
Sbjct: 465 GMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWD 524
Query: 506 EEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPK 565
E +C + EY N GPGA RR W K S +E + +TA+ WL GP
Sbjct: 525 GES------FHKSCRYVEYNNRGPGAFANRRANWA--KVARSAAEVSGFTAANWL--GP- 573
Query: 566 TAPKSAVEWLHDLHVPHYLG 585
+ W+ + +VP +G
Sbjct: 574 ------INWIQEANVPVTIG 587
>emb|CAA73733.1| pectin methylesterase-like protein [Zea mays]
gi|7447384|pir||T04359 pectin methylesterase-like
protein - maize
Length = 563
Score = 377 bits (968), Expect = e-103
Identities = 231/593 (38%), Positives = 328/593 (54%), Gaps = 42/593 (7%)
Query: 4 KVIISVVSLILVVGVIIGVVVDIRKNGE---DPKVQTQQRNLRIMCQNAQDQKLCHDTLS 60
K ++ +S ILVV V++GVV + ++G+ D + +L + Q Q H
Sbjct: 3 KALLGGLSAILVVAVVVGVVATVTRSGKKAGDNFTVPGEASLANVRQVGQVPVRAHPVQG 62
Query: 61 SVRGADAA------DPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKD 114
VR +P+ + + + G + + A +DCK
Sbjct: 63 VVREDVVPGHQWHREPQGGVPQRGQGGAGVGPDGGRAVQVDRRGQGSDSMTESAREDCKK 122
Query: 115 LMQFALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIK 174
L++ A D L + +I+ + ++ D+ WL+ V+++ C++GF D +K+K
Sbjct: 123 LLEDAADDLRGMLE-MAGGDIKVLFSRSDDLETWLTGVMTFMDTCVDGFVD-----EKLK 176
Query: 175 EQFHVQSLYSVQKVTAVALDIVTGLSDILQQFNLNFDIKPPSRRLLNSEVTVDDQGYPSW 234
H + + ++++ AL I L IL++ +L K RRLL+SE D++G+P W
Sbjct: 177 ADMH-SVVRNATELSSNALAITNSLGGILKKMDLGMFSKDSRRRLLSSEQ--DEKGWPVW 233
Query: 235 ISSSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKAG 294
+ S RKLLA + +PNA+VA DGSGQFK+IQ A+ + PKG++ RYVIYVKAG
Sbjct: 234 MRSPERKLLASGNQP------KPNAIVAKDGSGQFKSIQQAVDAVPKGHQGRYVIYVKAG 287
Query: 295 VYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFE 354
+YDE + VPK+ VNI MYGDGP ++ VTGRK+ G T TATFS A GFI K M F
Sbjct: 288 LYDEIVMVPKDKVNIFMYGDGPKQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFH 347
Query: 355 NTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSA 414
NTAG QAVA R GD++A C +QDTLYVQ RQF+RNCV+SGTIDFIFG SA
Sbjct: 348 NTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVQPRRQFFRNCVVSGTIDFIFGNSA 407
Query: 415 TLIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFTVRSY 474
+ Q+ II R+ N+ N + A G NM +G+VIQ+C ++P+ L P++F + SY
Sbjct: 408 AVFQNCLIITRRPM--DNQQNSVTAHGPTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSY 465
Query: 475 LGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANVA 534
LGRPW+ S+ VIMESTI DF+ +G+ W + T Y+AEY N GPGA +
Sbjct: 466 LGRPWKEFSRLVIMESTIADFVKPEGYMPWNGD------FALKTLYYAEYNNRGPGAGTS 519
Query: 535 RRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHYLGFK 587
+RV W G+ VI R EA +TA ++D WL PH LGFK
Sbjct: 520 KRVNWPGFH-VIGRKEAEPFTAGPFID---------GAMWLKYTGAPHILGFK 562
>pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilgiana
gi|6714532|dbj|BAA89480.1| pectin methylesterase [Salix
gilgiana]
Length = 596
Score = 370 bits (951), Expect = e-101
Identities = 234/582 (40%), Positives = 321/582 (54%), Gaps = 54/582 (9%)
Query: 1 MKGKVIISVVSLILVVGVIIGVVV-------DIRK---NGEDPKVQTQQ----------- 39
++ K+II+ VS I VI+G V DI NG P T+
Sbjct: 21 LRKKIIIASVSSIAFF-VIVGAGVFSLVSNHDISSPGNNGGSPSAATRPAEKAKPISHVA 79
Query: 40 RNLRIMCQNAQDQKLCHDTLSS-VRGAD--AADPKAYIAAAVKAATDNVIKAFNMSERLT 96
R ++ +C Q C +TL V G D + PK + A+KAA + + K +
Sbjct: 80 RVIKTVCNATTYQDTCQNTLEKGVLGKDPSSVQPKDLLKIAIKAADEEIDKVIKKASSFK 139
Query: 97 TEYGKKNGAKMALDDCKDLMQFALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYK 156
+ K K A DDC +L++ A + L S +C+ N+I + D+ NWLSAV+SY+
Sbjct: 140 FD---KPREKAAFDDCLELIEDAKEELKNSVDCI-GNDIGKLASNAPDLSNWLSAVMSYQ 195
Query: 157 QGCMEGFDDANDGEKKIKEQFHVQSLYSVQKVTAVALDIVTGLSDILQQFNLNFDIKPPS 216
Q C++GF E K+K ++ + +++T+ +L +V+ L L+ F+ + + +
Sbjct: 196 QTCIDGFP-----EGKLKSDME-KTFKATRELTSNSLAMVSSLVSFLKNFSFSGTL---N 246
Query: 217 RRLLNSEV---TVDDQGYPSWISSSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQ 273
RRLL E ++D G P W+S R++L + +PN VA DGSG FKTI
Sbjct: 247 RRLLAEEQNSPSLDKDGVPGWMSHEDRRILKGADKD----KPKPNVSVAKDGSGDFKTIS 302
Query: 274 AALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNT 333
ALA+ P + RYVI+VK GVYDE +TV K+ NI MYGDG KTIVTG KN G T
Sbjct: 303 EALAAMPAKYEGRYVIFVKQGVYDETVTVTKKMANITMYGDGSQKTIVTGNKNFADGVQT 362
Query: 334 QNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTN 393
TATF+ GF+ K M F NTAGP QAVA R D + + C GYQDTLY QT+
Sbjct: 363 FRTATFAVLGDGFLCKFMGFRNTAGPEKHQAVAIRVQADRAIFLNCRFEGYQDTLYAQTH 422
Query: 394 RQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVI 453
RQFYR+CVI+GT+DFIFG + ++ Q+ I VRK N+ N++ A G + TGIV+
Sbjct: 423 RQFYRSCVITGTVDFIFGDATSVFQNCLITVRKPL--ENQQNIVTAQGRIDGHETTGIVL 480
Query: 454 QDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEK 513
Q C I P+ LVP K +RSYLGRPW+ S+ VIM+STIGDFIH GW W +
Sbjct: 481 QSCRIEPDKDLVPVKNKIRSYLGRPWKEFSRTVIMDSTIGDFIHPGGWLPWQGDFGL--- 537
Query: 514 HHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYT 555
T Y+AEY+N G GA R+KW GY +I + EA K+T
Sbjct: 538 ---KTLYYAEYSNKGGGAQTNARIKWPGYH-IIKKEEAMKFT 575
>emb|CAA39658.1| Bp19 [Brassica napus] gi|99823|pir||S14952 pectinesterase homolog -
rape gi|1172538|sp|P41510|PME_BRANA Probable
pectinesterase precursor (Pectin methylesterase) (PE)
Length = 584
Score = 351 bits (901), Expect = 3e-95
Identities = 222/612 (36%), Positives = 329/612 (53%), Gaps = 61/612 (9%)
Query: 3 GKVIISVVSLILVVGVIIGVVVDIRKNGE---DPKVQTQQRNLRIMCQNAQDQKLCHDTL 59
GK++ISV S++LVVGV IGVV + K G D + + Q+ + +C +A D+ C TL
Sbjct: 4 GKIVISVASMLLVVGVAIGVVTFVNKGGGAGGDKTLNSHQKAVESLCASATDKGSCAKTL 63
Query: 60 SSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGA--KMALDDCKDLMQ 117
V+ + DP I A + A D V K+ N + GK A K LD CK ++
Sbjct: 64 DPVK---SDDPSKLIKAFMLATKDAVTKSTNFTASTEEGMGKNINATSKAVLDYCKRVLM 120
Query: 118 FALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQF 177
+AL+ L+ + ++ ++ + ++ WL+ V +Y+ C+ DD + E + +
Sbjct: 121 YALEDLETIVEEMGED-LQQSGSKMDQLKQWLTGVFNYQTDCI---DDIEESELR---KV 173
Query: 178 HVQSLYSVQKVTAVALDIVTGLSDILQQFNLNFD---------IKPPSRRLLNSEVTVDD 228
+ + + +++ A+DI L+ + Q N+ D P R LL +D
Sbjct: 174 MGEGIAHSKILSSNAIDIFHALTTAMSQMNVKVDDMKKGNLGETPAPDRDLLED---LDQ 230
Query: 229 QGYPSWISSSGRKLLAKMQRKGWRAN------------IRPNAVVANDGSGQFKTIQAAL 276
+G P W S RKL+A+ R G A+ I+P VVA DGSGQFKTI A+
Sbjct: 231 KGLPKWHSDKDRKLMAQAGRPGAPADEGIGEGGGGGGKIKPTHVVAKDGSGQFKTISEAV 290
Query: 277 ASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQ--MAGTNTQ 334
+ P+ N R +IY+KAGVY E +T+PK+ N+ M+GDG +TI+T ++ GT T
Sbjct: 291 KACPEKNPGRCIIYIKAGVYKEQVTIPKKVNNVFMFGDGATQTIITFDRSVGLSPGTTTS 350
Query: 335 NTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNR 394
+ T + GF+ K + F+NTAGP G QAVAFR GD + + C GYQDTLYV R
Sbjct: 351 LSGTVQVESEGFMAKWIGFQNTAGPLGHQAVAFRVNGDRAVIFNCRFDGYQDTLYVNNGR 410
Query: 395 QFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVN-MNTGIVI 453
QFYRN V+SGT+DFIFG SAT+IQ+S I+ RKG+ + N + ADG+ + GIV+
Sbjct: 411 QFYRNIVVSGTVDFIFGKSATVIQNSLILCRKGS--PGQTNHVTADGNEKGKAVKIGIVL 468
Query: 454 QDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEK 513
+C I+ + L ++ TV+SYLGRPW+ + ++ + IGD I GW W + EK
Sbjct: 469 HNCRIMADKELEADRLTVKSYLGRPWKPFATTAVIGTEIGDLIQPTGWNEW-----QGEK 523
Query: 514 HHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVE 573
H T + E+ N GPGAN A RV W K S +E ++T + WL +
Sbjct: 524 FH-LTATYVEFNNRGPGANTAARVPWA--KMAKSAAEVERFTVANWL---------TPAN 571
Query: 574 WLHDLHVPHYLG 585
W+ + +VP LG
Sbjct: 572 WIQEANVPVQLG 583
>emb|CAB71877.1| PECTINESTERASE-like protein [Arabidopsis thaliana]
gi|21536813|gb|AAM61145.1| PECTINESTERASE-like protein
[Arabidopsis thaliana] gi|20260676|gb|AAM13236.1|
pectinesterase-like protein [Arabidopsis thaliana]
gi|61213924|sp|Q5MFV6|PMEL_ARATH Probable pectinesterase
VGDH2 precursor (Pectin methylesterase) (PE)
(VANGUARD1-like protein 2) (VGD1-like protein 2)
gi|15228697|ref|NP_191776.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 588
Score = 350 bits (897), Expect = 9e-95
Identities = 218/612 (35%), Positives = 321/612 (51%), Gaps = 57/612 (9%)
Query: 3 GKVIISVVSLILVVGVIIGVVVDIRKNG-----EDPKVQTQQRNLRIMCQNAQDQKLCHD 57
GKV++SV SL+LVVGV IGV+ + K G + + + Q+ ++ +CQ+ DQ C
Sbjct: 4 GKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQGSCAK 63
Query: 58 TLSSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGA--KMALDDCKDL 115
TL V+ + DP + A + A D + K+ N + G A K LD CK +
Sbjct: 64 TLDPVK---SDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRV 120
Query: 116 MQFALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKE 175
+ +AL+ L+ + ++ ++ + ++ WL+ V +Y+ C++ ++ KKI
Sbjct: 121 LMYALEDLETIVEEMGED-LQQSGTKLDQLKQWLTGVFNYQTDCLDDIEEVE--LKKIMG 177
Query: 176 QFHVQSLYSVQKVTAVALDIVTGLS------DILQQFNLNFDIKPPSRRLLNSEVTVDDQ 229
+ S + +VT ++ D ++ + +RRLL D +
Sbjct: 178 EGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDN---DSK 234
Query: 230 GYPSWISSSGRKLLAKMQRK-------------GWRANIRPNAVVANDGSGQFKTIQAAL 276
G P W S RKL+AK R G I+ VVA DGSGQFKTI A+
Sbjct: 235 GLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEAV 294
Query: 277 ASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMA--GTNTQ 334
+ P N R +I++KAG+Y+E + +PK+ NI M+GDG +TI+T ++ GT T
Sbjct: 295 MACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPGTTTS 354
Query: 335 NTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNR 394
+ T + GF+ K + F+NTAGP G QAVA R GD + + C GYQDTLYV R
Sbjct: 355 LSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYVNNGR 414
Query: 395 QFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPL-VNMNTGIVI 453
QFYRN V+SGT+DFIFG SAT+IQ+S I+VRKG+ + Y + ADG+ M GIV+
Sbjct: 415 QFYRNIVVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNY--VTADGNEKGAAMKIGIVL 472
Query: 454 QDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEK 513
+C IIP+ L +K T++SYLGRPW+ + VI+ + IGD I +GWT W EQN
Sbjct: 473 HNCRIIPDKELEADKLTIKSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQN---- 528
Query: 514 HHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVE 573
T + E+ N GPGA +R W K S +E YT + W+ GP
Sbjct: 529 --HKTAKYIEFNNRGPGAATTQRPPW--VKVAKSAAEVETYTVANWV--GP-------AN 575
Query: 574 WLHDLHVPHYLG 585
W+ + +VP LG
Sbjct: 576 WIQEANVPVQLG 587
>gb|AAV91510.1| VGD1-like protein 2 [Arabidopsis thaliana]
Length = 588
Score = 345 bits (886), Expect = 2e-93
Identities = 217/612 (35%), Positives = 319/612 (51%), Gaps = 57/612 (9%)
Query: 3 GKVIISVVSLILVVGVIIGVVVDIRKNG-----EDPKVQTQQRNLRIMCQNAQDQKLCHD 57
GKV++SV SL+LVVGV IGV+ + K G + + + Q+ ++ +C + DQ C
Sbjct: 4 GKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICLSTTDQGSCAK 63
Query: 58 TLSSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGA--KMALDDCKDL 115
TL V+ + DP + A + A D + K+ N + G A K LD CK +
Sbjct: 64 TLDPVK---SDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRV 120
Query: 116 MQFALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKE 175
+ +AL+ L+ + ++ ++ + ++ WL+ V +Y+ C+ ++ KKI
Sbjct: 121 LMYALEDLETIVEEMGED-LQQSGTKLDQLKQWLTGVFNYQTDCLGDIEEVE--LKKIMG 177
Query: 176 QFHVQSLYSVQKVTAVALDIVTGLS------DILQQFNLNFDIKPPSRRLLNSEVTVDDQ 229
+ S + +VT ++ D ++ + +RRLL D +
Sbjct: 178 EGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDN---DSK 234
Query: 230 GYPSWISSSGRKLLAKMQRK-------------GWRANIRPNAVVANDGSGQFKTIQAAL 276
G P W S RKL+AK R G I+ VVA DGSGQFKTI A+
Sbjct: 235 GLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEAV 294
Query: 277 ASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMA--GTNTQ 334
+ P N R +I++KAG+Y+E + +PK+ NI M+GDG +TI+T ++ GT T
Sbjct: 295 MACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPGTTTS 354
Query: 335 NTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNR 394
+ T + GF+ K + F+NTAGP G QAVA R GD + + C GYQDTLYV R
Sbjct: 355 LSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYVNNGR 414
Query: 395 QFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPL-VNMNTGIVI 453
QFYRN V+SGT+DFIFG SAT+IQ+S I+VRKG+ + Y + ADG+ M GIV+
Sbjct: 415 QFYRNIVVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNY--VTADGNEKGAAMKIGIVL 472
Query: 454 QDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEK 513
+C IIP+ L +K T++SYLGRPW+ + VI+ + IGD I +GWT W EQN
Sbjct: 473 HNCRIIPDKELEADKLTIKSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQN---- 528
Query: 514 HHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVE 573
T + E+ N GPGA +R W K S +E YT + W+ GP
Sbjct: 529 --HKTAKYIEFNNRGPGAATTQRPPW--VKVAKSAAEVETYTVANWV--GP-------AN 575
Query: 574 WLHDLHVPHYLG 585
W+ + +VP LG
Sbjct: 576 WIQEANVPVQLG 587
>ref|XP_474065.1| OSJNBb0079B02.7 [Oryza sativa (japonica cultivar-group)]
gi|32490042|emb|CAE05961.1| OSJNBa0063C18.2 [Oryza
sativa (japonica cultivar-group)]
gi|38344904|emb|CAE02974.2| OSJNBb0079B02.7 [Oryza
sativa (japonica cultivar-group)]
Length = 971
Score = 333 bits (853), Expect = 1e-89
Identities = 179/385 (46%), Positives = 234/385 (60%), Gaps = 38/385 (9%)
Query: 215 PSRRLLNSEV--TVDD-----------QGYPSWISSSGRKLLAKMQRKGWRANIRPNAVV 261
P RLL ++V T++D +PSW+S+ R+LL +K P+ VV
Sbjct: 610 PHHRLLTTDVVGTIEDIEHERHNQPKPGEFPSWVSAHQRRLLQAGTQK-------PDKVV 662
Query: 262 ANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIV 321
A DGSG FKTI A+ + PK + R+VIYVKAG Y+EY+T+P NI MYGDGP KT V
Sbjct: 663 AKDGSGDFKTITEAVNAVPKNSPTRFVIYVKAGEYNEYVTIPSSLPNIFMYGDGPTKTRV 722
Query: 322 TGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHI 381
G K+ G T T TFS GF+ K+M F NTAGP G QAVA GDMS C
Sbjct: 723 LGNKSNKDGVATMATRTFSAEGNGFVCKSMGFVNTAGPEGHQAVALHVQGDMSVFFNCKF 782
Query: 382 VGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADG 441
GYQDTLYV NRQF+RNC ++GTID+IFG SA + QS + VRK N+ N++ A G
Sbjct: 783 EGYQDTLYVHANRQFFRNCEVTGTIDYIFGNSAAVFQSCLMTVRKPM--DNQANMVTAHG 840
Query: 442 SPLVNMNTGIVIQDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGW 501
NM TGIV+QDC I+PE AL P + + SYLGRPW+ ++ V+MES IGDFI +GW
Sbjct: 841 RTDPNMPTGIVLQDCRIVPEQALFPVRLQIASYLGRPWKEYARTVVMESVIGDFIKPEGW 900
Query: 502 TTWPEEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLD 561
+ W + T Y+AEYANTGPGA ++RV W GY+ VI ++EAT++TA +++D
Sbjct: 901 SEWMGDVGL------KTLYYAEYANTGPGAGTSKRVTWPGYR-VIGQAEATQFTAGVFID 953
Query: 562 AGPKTAPKSAVEWLHDLHVPHYLGF 586
+ WL + P+ +GF
Sbjct: 954 ---------GLTWLKNTATPNVMGF 969
Score = 64.7 bits (156), Expect = 8e-09
Identities = 57/202 (28%), Positives = 99/202 (48%), Gaps = 23/202 (11%)
Query: 6 IISVVSLILVVGVIIGVVVDIR-KNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTLSSVRG 64
I++ V ++ VV I V + +NGE T L +C + C +L V
Sbjct: 10 IVAAVGVVAVVCAIAAVTSSKKDRNGE----LTANVRLSTVCSVTRYPGRCEQSLGPVVN 65
Query: 65 ADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKM---ALDDCKDLMQFALD 121
D DP++ + AA++ A + V AFN S + GK + AK+ A++ CK L+ A++
Sbjct: 66 -DTIDPESVLRAALQVALEEVTSAFNRS----MDVGKDDDAKITKSAIEMCKKLLDDAIE 120
Query: 122 SLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFHVQS 181
L + E V D+R WLS+V++Y C +GFD + ++KE +
Sbjct: 121 DL----RGMASLKPEEVTKHVNDLRCWLSSVMTYIYTCADGFD-----KPELKEAMD-KL 170
Query: 182 LYSVQKVTAVALDIVTGLSDIL 203
L + ++++ AL I+T L +++
Sbjct: 171 LQNSTELSSNALAIITSLGELM 192
>emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (indica
cultivar-group)]
Length = 717
Score = 333 bits (853), Expect = 1e-89
Identities = 179/385 (46%), Positives = 234/385 (60%), Gaps = 38/385 (9%)
Query: 215 PSRRLLNSEV--TVDD-----------QGYPSWISSSGRKLLAKMQRKGWRANIRPNAVV 261
P RLL ++V T++D +PSW+S+ R+LL +K P+ VV
Sbjct: 356 PHHRLLTTDVVGTIEDIEHERHNQPKPGEFPSWVSAHQRRLLQAGTQK-------PDKVV 408
Query: 262 ANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIV 321
A DGSG FKTI A+ + PK + R+VIYVKAG Y+EY+T+P NI MYGDGP KT V
Sbjct: 409 AKDGSGDFKTITEAVNAVPKNSPTRFVIYVKAGEYNEYVTIPSSLPNIFMYGDGPTKTRV 468
Query: 322 TGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHI 381
G K+ G T T TFS GF+ K+M F NTAGP G QAVA GDMS C
Sbjct: 469 LGNKSNKDGVATMATRTFSAEGNGFVCKSMGFVNTAGPEGHQAVALHVQGDMSVFFNCKF 528
Query: 382 VGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADG 441
GYQDTLYV NRQF+RNC ++GTID+IFG SA + QS + VRK N+ N++ A G
Sbjct: 529 EGYQDTLYVHANRQFFRNCEVTGTIDYIFGNSAAVFQSCLMTVRKPM--DNQANMVTAHG 586
Query: 442 SPLVNMNTGIVIQDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGW 501
NM TGIV+QDC I+PE AL P + + SYLGRPW+ ++ V+MES IGDFI +GW
Sbjct: 587 RTDPNMPTGIVLQDCRIVPEQALFPVRLQIASYLGRPWKEYARTVVMESVIGDFIKPEGW 646
Query: 502 TTWPEEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLD 561
+ W + T Y+AEYANTGPGA ++RV W GY+ VI ++EAT++TA +++D
Sbjct: 647 SEWMGDVGL------KTLYYAEYANTGPGAGTSKRVTWPGYR-VIGQAEATQFTAGVFID 699
Query: 562 AGPKTAPKSAVEWLHDLHVPHYLGF 586
+ WL + P+ +GF
Sbjct: 700 ---------GLTWLKNTATPNVMGF 715
>ref|NP_198139.1| pectinesterase family protein [Arabidopsis thaliana]
Length = 732
Score = 333 bits (853), Expect = 1e-89
Identities = 211/589 (35%), Positives = 308/589 (51%), Gaps = 46/589 (7%)
Query: 2 KGKVIISVVSLIL---VVGVIIGVVVDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDT 58
K VIIS+ S++L VV V IGV V+ N D ++ T + ++ +C ++ C DT
Sbjct: 14 KRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDT 73
Query: 59 LSSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDLMQF 118
L D +DP + A A + S+ + E K AKMALD CK+LM +
Sbjct: 74 LRK-DAKDTSDPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 131
Query: 119 ALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFD--DANDGEKKIKEQ 176
A+ L S + V + +R WLSA IS++Q C++GF N GE K
Sbjct: 132 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKK-- 189
Query: 177 FHVQSLYSVQKVTAVALDIVTGLSDILQQFNLNFDIKPPSRRLLNSEVTVDDQGYPSWIS 236
+L + ++T L +VT +S+ L Q + + SRRLL+ E +PSW+
Sbjct: 190 ----ALKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLSQE-------FPSWMD 235
Query: 237 SSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGVY 296
+ R+LL + ++P+ VVA DGSGQ+KTI AL PK +V+++K G+Y
Sbjct: 236 ARARRLLNAPM-----SEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIY 290
Query: 297 DEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFENT 356
EY+ V + +++ GDGP KT+++G K+ G T TAT + FI K + FENT
Sbjct: 291 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 350
Query: 357 AGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSATL 416
AG QAVA R + D S C GYQDTLY ++RQFYR+C ISGTIDF+FG +A +
Sbjct: 351 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 410
Query: 417 IQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFTVRSYLG 476
Q+ T++VRK N+ I A G +TG V+Q C I+ E + K ++YLG
Sbjct: 411 FQNCTLLVRKPLL--NQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLG 468
Query: 477 RPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANVARR 536
RPW+ S+ +IM + I DF+ +GW W E NT +++E NTGPGA + +R
Sbjct: 469 RPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGL------NTLFYSEVQNTGPGAAITKR 522
Query: 537 VKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHYLG 585
V W G K +S E K+T + ++ W+ VP+ LG
Sbjct: 523 VTWPGIK-KLSDEEILKFTPAQYIQGD---------AWIPGKGVPYILG 561
>emb|CAB65290.2| pectin methyl-esterase PER [Medicago truncatula]
Length = 602
Score = 330 bits (847), Expect = 6e-89
Identities = 200/551 (36%), Positives = 300/551 (54%), Gaps = 52/551 (9%)
Query: 6 IISVVSLILVVGVIIGVVVDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTLSSVRGA 65
++ V S++LV ++ V D Q Q N++I+C++ Q Q+ CH +L+ A
Sbjct: 18 LVGVSSILLVA--MVATVAD---------AQQGQPNVQILCESTQYQQTCHQSLAKAP-A 65
Query: 66 DAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDLMQFALDSLDL 125
+ A K I AA A ++ ++K N S L E G+ K A++ C +++ +A+D +
Sbjct: 66 ETAGVKDLIKAAFSATSEELLKHINSSS-LIQELGQDKMTKQAMEVCNEVLDYAVDGIHK 124
Query: 126 SNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFHVQSLYSV 185
S V +I +H+ + D++ WL+ +S++Q C++GF AN K + ++L +
Sbjct: 125 SVGAVDKFDINKIHEYSYDLKVWLTGTLSHQQTCLDGF--ANTTTKAGETM--ARALNTS 180
Query: 186 QKVTAVALDIVTGLSDILQQFNLNFDIKPPSRRLLNSEVTVDDQGYPSWISSSGRKLLAK 245
++++ A+D+V + +D+ RRLL+ D GYP W+S R+LLA+
Sbjct: 181 IQLSSNAIDMVDAV----------YDLTNAKRRLLSL-----DNGYPLWVSEGQRRLLAE 225
Query: 246 MQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKE 305
A ++PN VVA DGSGQFKT+ A+ + P N +VIYVK GVY+E + VPK+
Sbjct: 226 -------ATVKPNVVVAQDGSGQFKTLTDAIKTVPANNAQNFVIYVKEGVYNETVNVPKD 278
Query: 306 AVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAV 365
+ + GDGPAKT TG N G NTATF F+ K ++ ENTAGP QAV
Sbjct: 279 MAFVTIIGDGPAKTKFTGSLNYADGLLPYNTATFGVNGENFMAKDISIENTAGPEKHQAV 338
Query: 366 AFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVR 425
A R D + C I GYQ TL+ ++ RQFYR+C ISGTID I+G + + Q+ +IVR
Sbjct: 339 ALRVTADKAIFYNCQIDGYQATLFAESQRQFYRDCSISGTIDMIYGDAFAVFQNCKLIVR 398
Query: 426 KGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNII--PEAALVPEKFTVRSYLGRPWQNES 483
K + + + ADG + ++G V Q C+ PE A + K +YLGRPW++ S
Sbjct: 399 KPLEEQQCF--VAADGRTKSDSSSGFVFQSCHFTGEPEVAKIDPKI---AYLGRPWKSYS 453
Query: 484 KAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYK 543
K VIM+S I D +G+ W K +TC F EY N GPGA+ ++RVKW G K
Sbjct: 454 KVVIMDSNIDDIFDPEGYMPWMGSAFK------DTCTFYEYNNKGPGADTSKRVKWPGVK 507
Query: 544 GVISRSEATKY 554
+ S A Y
Sbjct: 508 SISSTEAAAFY 518
>dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
Length = 617
Score = 330 bits (847), Expect = 6e-89
Identities = 207/552 (37%), Positives = 295/552 (52%), Gaps = 43/552 (7%)
Query: 30 GEDPKVQTQQRNLRIMCQNAQDQKLCHDTLSSVRGADAADPKAYIAAAVKAATDNVIKAF 89
G ++ +++++MC C ++ A + PK I AV V +AF
Sbjct: 85 GGKADLRAVSKSIKMMCAQTDFADSCATSIGKAANASVSSPKDIIRTAVDVIGGAVDQAF 144
Query: 90 NMSERLTTEYGKKNGAKMALDDCKDLMQFALDSLDLSNNC-VRDNNIEAVHDQTADMRNW 148
+ ++ + + + K A+ DCK+L A D L NC ++ + + Q +R W
Sbjct: 145 DRADLIMSNDPR---VKAAVADCKELFDDAKDDL----NCTLKGIDGKDGLKQGFQLRVW 197
Query: 149 LSAVISYKQGCMEGFDDANDGEKKIKEQFHVQSLYSVQKVTAVALDIVTGLSDILQQFNL 208
LSAVI+ + C++GF D + K+KE F+ + ++ T+ AL ++ S L
Sbjct: 198 LSAVIANMETCIDGFPDG-EFRDKVKESFN-----NGREFTSNALALIEKASSFLSA--- 248
Query: 209 NFDIKPPSRRLLNSE-----------VTVDDQGYPSWISSSGRKLLAKMQRKGWRANIRP 257
+K RRLL E + + + G P W+ R++L + G++ N+ P
Sbjct: 249 ---LKGSQRRLLAGEEDNGGGAADPHLALAEDGIPEWVPDGDRRVL---KGGGFKNNLTP 302
Query: 258 NAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGDGPA 317
N +VA DGSG+FKTI ALA+ PK RYVIYVK GVY EY+T+ K+ ++ MYGDG
Sbjct: 303 NVIVAKDGSGKFKTINEALAAMPKTYSGRYVIYVKEGVYAEYVTITKKMASVTMYGDGSR 362
Query: 318 KTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMSALV 377
K+IVTG KN G T TATF+ GF+ M F+NTAG A QAVA D S +
Sbjct: 363 KSIVTGSKNFADGLTTFKTATFAAQGDGFMAIGMGFQNTAGAAKHQAVALLVQSDKSVFL 422
Query: 378 GCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVI 437
C + G+QDTLY + QFYRNCVI+GTIDF+FG +A + Q+ + +R+ N+ N+
Sbjct: 423 NCWMDGFQDTLYAHSKAQFYRNCVITGTIDFVFGDAAAVFQNCVLTLRRPM--DNQQNIA 480
Query: 438 VADGSPLVNMNTGIVIQDCNIIPEAALVPEKF-TVRSYLGRPWQNESKAVIMESTIGDFI 496
A G TG V+Q C E AL K +R+YLGRPW+ S+ VIMES I I
Sbjct: 481 TAQGRADGREATGFVLQKCEFNAEPALTDAKLPPIRNYLGRPWREFSRTVIMESDIPAII 540
Query: 497 HQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKYTA 556
+ G+ W E T Y+AEYAN GPGA+ A RV W GYK VIS+++ATK+T
Sbjct: 541 DKAGYMPWNGE------FALKTLYYAEYANKGPGADTAGRVAWPGYKKVISKADATKFTV 594
Query: 557 SIWLDAGPKTAP 568
+L A P P
Sbjct: 595 DNFLHAKPWIDP 606
>pir||T52325 pectinesterase (EC 3.1.1.11) [imported] - turnip (fragment)
gi|1448939|gb|AAB04617.1| pectinesterase
gi|6093741|sp|Q42608|PME_BRACM Pectinesterase (Pectin
methylesterase) (PE)
Length = 571
Score = 330 bits (847), Expect = 6e-89
Identities = 214/602 (35%), Positives = 317/602 (52%), Gaps = 61/602 (10%)
Query: 13 ILVVGVIIGVVVDIRKNGE---DPKVQTQQRNLRIMCQNAQDQKLCHDTLSSVRGADAAD 69
+LVVGV IGVV + K G D + + Q+ + +C +A D+ C TL V+ + D
Sbjct: 1 LLVVGVAIGVVTFVNKGGGAGGDKTLNSHQKAVESLCASATDKGSCAKTLDPVK---SDD 57
Query: 70 PKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGA--KMALDDCKDLMQFALDSLDLSN 127
P I A + A D V K+ N + GK A K LD CK ++ +AL+ L+
Sbjct: 58 PSKLIKAFMLATKDAVTKSTNFTASTEEGMGKNMNATSKAVLDYCKRVLMYALEDLETIV 117
Query: 128 NCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFHVQSLYSVQK 187
+ ++ ++ + ++ WL+ V +Y+ C+ DD + E + + + + +
Sbjct: 118 EEMGED-LQQSGSKMDQLKQWLTGVFNYQTDCI---DDIEESELR---KVMGEGIRHSKI 170
Query: 188 VTAVALDIVTGLSDILQQFNLNFD---------IKPPSRRLLNSEVTVDDQGYPSWISSS 238
+++ A+DI L+ + Q N+ D P R LL +D +G P W S
Sbjct: 171 LSSNAIDIFHALTTAMSQMNVKVDDMKKGNLGETPAPDRDLLED---LDQKGLPKWHSDK 227
Query: 239 GRKLLAKMQRKGWRAN------------IRPNAVVANDGSGQFKTIQAALASYPKGNKDR 286
RKL+A+ R G A+ I+P VVA DGSGQFKTI A+ + P+ N R
Sbjct: 228 DRKLMAQAGRPGAPADEGIGEGGGGGGKIKPTHVVAKDGSGQFKTISEAVKACPEKNPGR 287
Query: 287 YVIYVKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQ--MAGTNTQNTATFSNTAM 344
+IY+KAGVY E +T+PK+ N+ M+GDG +TI+T ++ GT T + T +
Sbjct: 288 CIIYIKAGVYKEQVTIPKKVNNVFMFGDGATQTIITFDRSVGLSPGTTTSLSGTVQVESE 347
Query: 345 GFIGKAMTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISG 404
GF+ K + F+NTAGP G QAVAFR GD + + C GYQDTLYV RQFYRN V+SG
Sbjct: 348 GFMAKWIGFQNTAGPLGNQAVAFRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNIVVSG 407
Query: 405 TIDFIFGTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVN-MNTGIVIQDCNIIPEAA 463
T+DFI G SAT+IQ+S I+ RKG+ + N + ADG + GIV+ +C I+ +
Sbjct: 408 TVDFINGKSATVIQNSLILCRKGS--PGQTNHVTADGKQKGKAVKIGIVLHNCRIMADKE 465
Query: 464 LVPEKFTVRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAE 523
L ++ TV+SYLGRPW+ + ++ + IGD I GW W + EK H T + E
Sbjct: 466 LEADRLTVKSYLGRPWKPFATTAVIGTEIGDLIQPTGWNEW-----QGEKFH-LTATYVE 519
Query: 524 YANTGPGANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHY 583
+ N GPGAN A RV W K S +E ++T + WL + W+ + +V
Sbjct: 520 FNNRGPGANPAARVPWA--KMAKSAAEVERFTVANWL---------TPANWIQEANVTVQ 568
Query: 584 LG 585
LG
Sbjct: 569 LG 570
>ref|XP_479611.1| putative pectin methylesterase [Oryza sativa (japonica
cultivar-group)] gi|34394412|dbj|BAC83510.1| putative
pectin methylesterase [Oryza sativa (japonica
cultivar-group)]
Length = 566
Score = 329 bits (843), Expect = 2e-88
Identities = 208/585 (35%), Positives = 300/585 (50%), Gaps = 39/585 (6%)
Query: 2 KGKVIISVVSLILVVGVIIGVVVDIRKNG----EDPKVQTQQRNLRIMCQNAQDQKLCHD 57
KG +I + S +LVV V+ V V KNG ED ++ T ++++ CQ Q+ C +
Sbjct: 3 KGAIIGA--STVLVVAVVAAVCVVSFKNGGGGKEDGELSTSVKSVKAFCQPTDYQQTCEE 60
Query: 58 TLSSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDLMQ 117
L G A+ P A ++ + KA + S L E AL +CK+L++
Sbjct: 61 ELGKAAGNGASSPTDLAKAMFAVTSEKISKAISESSTLE-ELKNDKRTSGALQNCKELLE 119
Query: 118 FALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGF-DDANDGEKKIKEQ 176
+A+D L S + + H D+R WLSA ++Y+ C++GF + D K+K
Sbjct: 120 YAVDDLKTSFEKLGGFEMTNFHKAVDDLRTWLSAALTYQGTCLDGFLNTTTDAADKMKS- 178
Query: 177 FHVQSLYSVQKVTAVALDIVTGLSDILQQFNLNFDIKPPSRRLLNSEVTVDDQGYPSWIS 236
+L S Q++T L +V S L N+ RRLL D G P W+S
Sbjct: 179 ----ALNSSQELTEDILAVVDQFSATLGSLNIG------RRRLLA------DDGMPVWMS 222
Query: 237 SSGRK-LLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGV 295
GR+ LL + +P+ VA DGSG KTI A+A P NK+RY IYVKAG
Sbjct: 223 EGGRRQLLEAAGPEAGPVEFKPDVTVAADGSGDVKTIGEAVAKVPPKNKERYTIYVKAGT 282
Query: 296 YDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFEN 355
Y+EY++V + A N+ M GDG KTI+TG KN T++TAT GF + +T EN
Sbjct: 283 YNEYVSVGRPATNVNMIGDGIGKTIITGNKNFKMNLTTKDTATMEAIGNGFFMRGITVEN 342
Query: 356 TAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSAT 415
TAGP QAVA R DM+ C GYQDTLY RQF+R+C +SGTIDFIFG S
Sbjct: 343 TAGPENHQAVALRAQSDMAVFYQCEFDGYQDTLYPHAQRQFFRDCTVSGTIDFIFGNSQV 402
Query: 416 LIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFT--VRS 473
++Q+ + RK N+ N+I A G G VI +C + P L EKFT V++
Sbjct: 403 VLQNCLLQPRKPM--DNQVNIITAQGRREKRSAGGTVIHNCTVAPHPDL--EKFTDKVKT 458
Query: 474 YLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANV 533
YL RPW+ S+ + +++ IG + GW W +T Y+AE N GPGA++
Sbjct: 459 YLARPWKEYSRTIFVQNEIGAVVDPVGWLEW------NGNFALDTLYYAEVDNHGPGADM 512
Query: 534 ARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDL 578
++R KWKG + + + ++T ++ G + PK V ++ L
Sbjct: 513 SKRAKWKGVQSLTYQDVQKEFTVEAFIQ-GQEFIPKFGVPYIPGL 556
>gb|AAF26136.1| putative pectinesterase [Arabidopsis thaliana]
gi|15230019|ref|NP_187212.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 669
Score = 327 bits (838), Expect = 7e-88
Identities = 205/595 (34%), Positives = 306/595 (50%), Gaps = 47/595 (7%)
Query: 2 KGKVIISVVSLILVVGVIIGVVVDIRKNGED------PKVQTQQRNLRIMCQNAQDQKLC 55
K + I+ +S +L++ +++ V V + N D +V + ++ +C +K C
Sbjct: 12 KRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTC 71
Query: 56 HDTLSSVRGADAADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDL 115
DTL G + DP + A + A S+ + E K + +MALD CK+L
Sbjct: 72 EDTLIK-NGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKEL 129
Query: 116 MQFALDSLDLSNNCVRDNNIEAVHDQTADMRNWLSAVISYKQGCMEGFD--DANDGEKKI 173
M +ALD L S + + + ++R WLSA IS+++ C+EGF N GE
Sbjct: 130 MDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMK 189
Query: 174 KEQFHVQSLYSVQKVTAVALDIVTGLSDILQQFNLNFDIKPPSRRLLNSEVTVDDQGYPS 233
K +L + ++T L I++ +S+ + Q + SRRLL +G+PS
Sbjct: 190 K------ALKTAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA-------EGFPS 233
Query: 234 WISSSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKA 293
W+ GRKLL Q ++++P+ VVA DGSGQ+KTI AL PK +V+++KA
Sbjct: 234 WVDQRGRKLL---QAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKA 290
Query: 294 GVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTF 353
G+Y EY+ V K +++ GDGP KTI++G KN G T TAT + FI K + F
Sbjct: 291 GLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGF 350
Query: 354 ENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTS 413
ENTAG QAVA R D S C GYQDTLY ++RQF+R+C ISGTIDF+FG +
Sbjct: 351 ENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDA 410
Query: 414 ATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFTVRS 473
A + Q+ T++VRK N+ I A G +TG V Q C I E + K T ++
Sbjct: 411 AAVFQNCTLLVRKPL--PNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKA 468
Query: 474 YLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANV 533
YLGRPW+ S+ +IM + I DF+ GW W + T +++E NTGPG+ +
Sbjct: 469 YLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGL------KTLFYSEVQNTGPGSAL 522
Query: 534 ARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHYLGFKA 588
A RV W G K +S + K+T + ++ +W+ VP+ G A
Sbjct: 523 ANRVTWAGIK-TLSEEDILKFTPAQYIQGD---------DWIPGKGVPYTTGLLA 567
>gb|AAB57670.1| pectinesterase [Citrus sinensis]
Length = 584
Score = 327 bits (837), Expect = 9e-88
Identities = 205/595 (34%), Positives = 323/595 (53%), Gaps = 49/595 (8%)
Query: 2 KGKVIISVVSLILVVGVIIGVV--VDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTL 59
K K+ +++ + +LVV +IG+V V+ RKN D + L+ C + + LC +
Sbjct: 27 KKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAI 86
Query: 60 SSVRGAD--AADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDLMQ 117
++V A K I ++ T V + ++L K+AL DC + +
Sbjct: 87 AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETID 146
Query: 118 FALDSLDLSNNCVRDN-NIEAVHDQTADMRNWLSAVISYKQGCMEGF--DDANDGEKKIK 174
LD L + + + N +++ D++ +SA ++ + C++GF DDAN K ++
Sbjct: 147 ETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDAN---KHVR 203
Query: 175 EQFHVQSLYSVQKVTAVALDIVTGLSD----ILQQFNLNFDIKPPSRRLLNSEVTVDDQG 230
+ ++ V+K+ + AL ++ ++D I++ N +R+L TVD G
Sbjct: 204 DALSDGQVH-VEKMCSNALAMIKNMTDTDMMIMRTSN--------NRKLTEETSTVD--G 252
Query: 231 YPSWISSSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIY 290
+P+W+S R+LL +++ PNAVVA DGSG FKT+ AA+A+ P+G RY+I
Sbjct: 253 WPAWLSPGDRRLLQS-------SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIR 305
Query: 291 VKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKA 350
+KAGVY E + V K+ NI+ GDG +TI+TG +N + G+ T +AT + GF+ +
Sbjct: 306 IKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARD 365
Query: 351 MTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIF 410
+TF+NTAGP+ QAVA R D+SA C ++ YQDTLYV +NRQF+ NC+I+GT+DFIF
Sbjct: 366 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 425
Query: 411 GTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFT 470
G +A ++Q+ I RK N + N++ A G N NTGIVIQ I + L P + +
Sbjct: 426 GNAAAVLQNCDIHARKPN--SGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGS 483
Query: 471 VRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPG 530
+YLGRPW+ S+ VIM+S+I D IH GW W NT ++ E+ N+G G
Sbjct: 484 FPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEW------DGNFALNTLFYGEHQNSGAG 537
Query: 531 ANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHYLG 585
A + RVKWKG++ + S +EA +T ++ + WL P LG
Sbjct: 538 AGTSGRVKWKGFRVITSATEAQAFTPGSFI---------AGSSWLGSTGFPFSLG 583
>ref|NP_850077.1| pectinesterase family protein [Arabidopsis thaliana]
Length = 614
Score = 326 bits (836), Expect = 1e-87
Identities = 210/619 (33%), Positives = 310/619 (49%), Gaps = 83/619 (13%)
Query: 4 KVIISVVSLILVVGVIIG---VVVDIRKNGEDPKVQTQQRN------------------- 41
++I+ VSL++VV I+G V K E + Q Q +N
Sbjct: 24 RIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQQAKNHNKSGSGNNVVKDSDKKSP 83
Query: 42 -------------------------LRIMCQNAQDQKLCHDTLSSV--RGADAADPKAYI 74
++ +C + ++C TL + +G +P ++
Sbjct: 84 SPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFL 143
Query: 75 AAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDLMQFALDSLDLSNNCVRDNN 134
+A++A +++ L TE K A++ CK L++ A + S N +
Sbjct: 144 KSAIEAVNEDLDLVLEKVLSLKTE---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTE 200
Query: 135 IEAVHDQTADMRNWLSAVISYKQGCMEGFDDANDGEKKIKEQFHVQSLYSVQKVTAVALD 194
+ + D+ +WLSAV+SY++ C++GF++ N +K + S+ S Q +T+ +L
Sbjct: 201 VNSFEKVVPDLESWLSAVMSYQETCLDGFEEGN-----LKSEVKT-SVNSSQVLTSNSLA 254
Query: 195 IVTGLSDILQQFNLNFDIKPPSRRLLNSEVTVDDQGYPSWISSSGRKLLAKMQRKGWRAN 254
++ ++ NL+ +K R LL+ PSW+S+ R++L + K
Sbjct: 255 LIKTFTE-----NLSPVMKVVERHLLDD--------IPSWVSNDDRRMLRAVDVKA---- 297
Query: 255 IRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIYVKAGVYDEYITVPKEAVNILMYGD 314
++PNA VA DGSG F TI AL + P+ + RY+IYVK G+YDEY+TV K+ N+ M GD
Sbjct: 298 LKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGD 357
Query: 315 GPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKAMTFENTAGPAGMQAVAFRNIGDMS 374
G KTIVTG K+ T TATF GF+ ++M F NTAGP G QAVA R D S
Sbjct: 358 GSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRS 417
Query: 375 ALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIFGTSATLIQSSTIIVRKGNYDHNEY 434
+ C GYQDTLY T+RQ+YR+CVI GTIDFIFG +A + Q+ I +RKG +
Sbjct: 418 IFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGL--PGQK 475
Query: 435 NVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFTVRSYLGRPWQNESKAVIMESTIGD 494
N + A G TG V+ +C I L P K +SYLGRPW+N S+ +IMES I +
Sbjct: 476 NTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIEN 535
Query: 495 FIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPGANVARRVKWKGYKGVISRSEATKY 554
I GW W E +T Y+AEY N G + RVKW G+K VI++ EA Y
Sbjct: 536 VIDPVGWLRWQETD-----FAIDTLYYAEYNNKGSSGDTTSRVKWPGFK-VINKEEALNY 589
Query: 555 TASIWLDAGPKTAPKSAVE 573
T +L +A S V+
Sbjct: 590 TVGPFLQGDWISASGSPVK 608
>sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methylesterase) (PE)
gi|2098705|gb|AAB57667.1| pectinesterase [Citrus
sinensis]
Length = 584
Score = 326 bits (836), Expect = 1e-87
Identities = 205/595 (34%), Positives = 323/595 (53%), Gaps = 49/595 (8%)
Query: 2 KGKVIISVVSLILVVGVIIGVV--VDIRKNGEDPKVQTQQRNLRIMCQNAQDQKLCHDTL 59
K K+ +++ + +LVV +IG+V V+ RKN D + L+ C + + LC +
Sbjct: 27 KKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAI 86
Query: 60 SSVRGAD--AADPKAYIAAAVKAATDNVIKAFNMSERLTTEYGKKNGAKMALDDCKDLMQ 117
++V A K I ++ T V + ++L K+AL DC + +
Sbjct: 87 AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETID 146
Query: 118 FALDSLDLSNNCVRDN-NIEAVHDQTADMRNWLSAVISYKQGCMEGF--DDANDGEKKIK 174
LD L + + + N +++ D++ +SA ++ + C++GF DDAN K ++
Sbjct: 147 ETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDAN---KHVR 203
Query: 175 EQFHVQSLYSVQKVTAVALDIVTGLSD----ILQQFNLNFDIKPPSRRLLNSEVTVDDQG 230
+ ++ V+K+ + AL ++ ++D I++ N +R+L TVD G
Sbjct: 204 DALSDGQVH-VEKMCSNALAMIKNMTDTDMMIMRTSN--------NRKLTEETSTVD--G 252
Query: 231 YPSWISSSGRKLLAKMQRKGWRANIRPNAVVANDGSGQFKTIQAALASYPKGNKDRYVIY 290
+P+W+S R+LL +++ PNAVVA DGSG FKT+ AA+A+ P+G RY+I
Sbjct: 253 WPAWLSPGDRRLLQS-------SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIR 305
Query: 291 VKAGVYDEYITVPKEAVNILMYGDGPAKTIVTGRKNQMAGTNTQNTATFSNTAMGFIGKA 350
+KAGVY E + V K+ NI+ GDG +TI+TG +N + G+ T +AT + GF+ +
Sbjct: 306 IKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARD 365
Query: 351 MTFENTAGPAGMQAVAFRNIGDMSALVGCHIVGYQDTLYVQTNRQFYRNCVISGTIDFIF 410
+TF+NTAGP+ QAVA R D+SA C ++ YQDTLYV +NRQF+ NC+I+GT+DFIF
Sbjct: 366 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 425
Query: 411 GTSATLIQSSTIIVRKGNYDHNEYNVIVADGSPLVNMNTGIVIQDCNIIPEAALVPEKFT 470
G +A ++Q+ I RK N + N++ A G N NTGIVIQ I + L P + +
Sbjct: 426 GNAAAVLQNCDIHARKPN--SGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGS 483
Query: 471 VRSYLGRPWQNESKAVIMESTIGDFIHQDGWTTWPEEQNKPEKHHENTCYFAEYANTGPG 530
+YLGRPW+ S+ VIM+S+I D IH GW W NT ++ E+ N+G G
Sbjct: 484 FPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEW------DGNFALNTLFYGEHQNSGAG 537
Query: 531 ANVARRVKWKGYKGVISRSEATKYTASIWLDAGPKTAPKSAVEWLHDLHVPHYLG 585
A + RVKWKG++ + S +EA +T ++ + WL P LG
Sbjct: 538 AGTSGRVKWKGFRVITSATEAQAFTPGSFI---------AGSSWLGSTGFPFSLG 583
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.317 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 986,418,413
Number of Sequences: 2540612
Number of extensions: 41449709
Number of successful extensions: 103703
Number of sequences better than 10.0: 370
Number of HSP's better than 10.0 without gapping: 305
Number of HSP's successfully gapped in prelim test: 65
Number of HSP's that attempted gapping in prelim test: 102238
Number of HSP's gapped (non-prelim): 449
length of query: 588
length of database: 863,360,394
effective HSP length: 134
effective length of query: 454
effective length of database: 522,918,386
effective search space: 237404947244
effective search space used: 237404947244
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 78 (34.7 bits)
Medicago: description of AC144563.4