BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144563.2 + phase: 0
(576 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultiva... 454 e-126
emb|CAA73733.1| pectin methylesterase-like protein [Zea mays] gi... 432 e-119
gb|AAC34241.1| putative pectinesterase [Arabidopsis thaliana] gi... 417 e-115
pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilg... 416 e-115
gb|AAC27719.1| flower-specific pectin methylesterase precursor [... 415 e-114
gb|AAV91509.1| VGD1-like protein 1 [Arabidopsis thaliana] 412 e-113
emb|CAA39658.1| Bp19 [Brassica napus] gi|99823|pir||S14952 pecti... 391 e-107
emb|CAB71877.1| PECTINESTERASE-like protein [Arabidopsis thalian... 390 e-107
gb|AAV91510.1| VGD1-like protein 2 [Arabidopsis thaliana] 385 e-105
ref|XP_474065.1| OSJNBb0079B02.7 [Oryza sativa (japonica cultiva... 371 e-101
emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (in... 371 e-101
pir||T52325 pectinesterase (EC 3.1.1.11) [imported] - turnip (fr... 369 e-100
gb|AAB57670.1| pectinesterase [Citrus sinensis] 366 e-100
sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methyles... 366 1e-99
emb|CAB80040.1| pectinesterase-like protein [Arabidopsis thalian... 365 2e-99
sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methyles... 364 5e-99
emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum] 362 2e-98
ref|NP_198139.1| pectinesterase family protein [Arabidopsis thal... 360 5e-98
gb|AAM65978.1| pectin methylesterase [Arabidopsis thaliana] gi|1... 360 9e-98
dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica ... 360 9e-98
>emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultivar-group)]
gi|50924778|ref|XP_472740.1| OSJNBa0036B21.20 [Oryza
sativa (japonica cultivar-group)]
Length = 568
Score = 454 bits (1168), Expect = e-126
Identities = 247/587 (42%), Positives = 357/587 (60%), Gaps = 32/587 (5%)
Query: 1 MSGKVIISAVSLILVVGVAIGVVVAVRKNGE----------DPEVQTQQRNLRIMCQNSQ 50
M+ K ++ V ILVV V +GVV V ++G + + T +++ +C +
Sbjct: 1 MAQKFLLGGVGAILVVAVVVGVVATVTRSGNKAGDNFTVPGEANLATSGKSVESLCAPTL 60
Query: 51 DQKLCHETLSSVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALN 110
++ C +TL++ +PK + K+A +++ A S+ + ++ + A
Sbjct: 61 YKESCEKTLTTATSG-TENPKEVFSTVAKSALESIKSAVEKSKAIGEAKTSDSMTESARE 119
Query: 111 DCKDLMQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGE 170
DCK L++ ++D L + +++ + + D+ +WL+ V+++ C +GF D
Sbjct: 120 DCKALLEDSVDDLRGMVEMA-GGDVKVLFSRSDDLEHWLTGVMTFMDTCADGFAD----- 173
Query: 171 KKIKEQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKF--DVKPASRRLLNSEVTVDD 228
+K+K H L + ++++ AL I L I ++ +L P R L+ + TV
Sbjct: 174 EKLKADMH-SVLRNASELSSNALAITNTLGAIFKKLDLDMFKGENPIHRSLIAEQETVG- 231
Query: 229 QGYPSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYV 288
G+PSW+ + DRKLLA R + PNAVVA+DGSGQFKTIQ A+ S PKG++GRYV
Sbjct: 232 -GFPSWMKAPDRKLLASGDRNRPQ----PNAVVAQDGSGQFKTIQEAVNSMPKGHQGRYV 286
Query: 289 IYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIG 348
IYVKAG+YDE + VPKD VNI MYGDGP R+ VTGRKSFA G+ TM+TATF+ A GFI
Sbjct: 287 IYVKAGLYDEIVMVPKDKVNIFMYGDGPKRSRVTGRKSFADGITTMKTATFSVEAAGFIC 346
Query: 349 KAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDF 408
K M F NTAG + HQAVA R GD+ A C +QD+LYV + RQ++RNC++SGT+DF
Sbjct: 347 KNMGFHNTAGAERHQAVALRINGDLGAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDF 406
Query: 409 IFGSSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFT 468
IFG+SA + Q+ II R+P Q N++TA G N+ +G+VIQ+C ++P+ LFP+RF
Sbjct: 407 IFGNSAAVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFK 466
Query: 469 IRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARR 528
I SYLGRPWK ++ V+MESTI DFI P+G+ W GE NT YYAE+ N GPGA ++R
Sbjct: 467 IPSYLGRPWKEYSRLVIMESTIADFIKPEGYMPWNGEFALNTLYYAEFNNRGPGAGTSKR 526
Query: 529 VKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLGFK 575
V WKG+ VI + EA +FTAG ++ G WL PH+LGFK
Sbjct: 527 VNWKGFR-VIGQKEAEQFTAGPFVDGG-----TWLKFTGTPHFLGFK 567
>emb|CAA73733.1| pectin methylesterase-like protein [Zea mays]
gi|7447384|pir||T04359 pectin methylesterase-like
protein - maize
Length = 563
Score = 432 bits (1112), Expect = e-119
Identities = 245/581 (42%), Positives = 344/581 (59%), Gaps = 30/581 (5%)
Query: 4 KVIISAVSLILVVGVAIGVVVAVRKNGE---DPEVQTQQRNLRIMCQNSQDQKLCHETLS 60
K ++ +S ILVV V +GVV V ++G+ D + +L + Q Q H
Sbjct: 3 KALLGGLSAILVVAVVVGVVATVTRSGKKAGDNFTVPGEASLANVRQVGQVPVRAHPVQG 62
Query: 61 SVHGADAA------DPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALNDCKD 114
V +P+ + + + G ++ + A DCK
Sbjct: 63 VVREDVVPGHQWHREPQGGVPQRGQGGAGVGPDGGRAVQVDRRGQGSDSMTESAREDCKK 122
Query: 115 LMQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIK 174
L++ A D L + +I+ + + D+ WL+ V+++ C++GF D +K+K
Sbjct: 123 LLEDAADDLRGMLEMA-GGDIKVLFSRSDDLETWLTGVMTFMDTCVDGFVD-----EKLK 176
Query: 175 EQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLLNSEVTVDDQGYPSW 234
H + + ++++ AL I L IL++ +L K + RRLL+SE D++G+P W
Sbjct: 177 ADMH-SVVRNATELSSNALAITNSLGGILKKMDLGMFSKDSRRRLLSSEQ--DEKGWPVW 233
Query: 235 ISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIYVKAG 294
+ S +RKLLA + PNA+VAKDGSGQFK+IQ A+ + PKG++GRYVIYVKAG
Sbjct: 234 MRSPERKLLASGNQPK------PNAIVAKDGSGQFKSIQQAVDAVPKGHQGRYVIYVKAG 287
Query: 295 VYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKAMTFE 354
+YDE + VPKD VNI MYGDGP ++ VTGRKSFA G+ TM+TATF+ A GFI K M F
Sbjct: 288 LYDEIVMVPKDKVNIFMYGDGPKQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFH 347
Query: 355 NTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFGSSA 414
NTAG + HQAVA R QGD++A C +QD+LYVQ RQ++RNC+VSGT+DFIFG+SA
Sbjct: 348 NTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVQPRRQFFRNCVVSGTIDFIFGNSA 407
Query: 415 TLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIRSYLG 474
+ Q+ II R+P Q N++TA G N+ +G+VIQ+C ++P+ LFP+RF I SYLG
Sbjct: 408 AVFQNCLIITRRPMDNQQNSVTAHGPTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLG 467
Query: 475 RPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVKWKGY 534
RPWK ++ V+MESTI DF+ P+G+ W G+ T YYAEY N GPGA ++RV W G+
Sbjct: 468 RPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNWPGF 527
Query: 535 HGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLGFK 575
H VI R EA FTAG ++ A WL PH LGFK
Sbjct: 528 H-VIGRKEAEPFTAGPFI-----DGAMWLKYTGAPHILGFK 562
>gb|AAC34241.1| putative pectinesterase [Arabidopsis thaliana]
gi|15450765|gb|AAK96654.1| putative pectinesterase
[Arabidopsis thaliana] gi|61213847|sp|O80722|PME4_ARATH
Pectinesterase-4 precursor (Pectin methylesterase 4) (PE
4) (VANGUARD1-like protein 1) (VGD1-like protein 1)
(AtPME4) gi|15226482|ref|NP_182226.1| pectinesterase
family protein [Arabidopsis thaliana]
Length = 588
Score = 417 bits (1072), Expect = e-115
Identities = 240/608 (39%), Positives = 349/608 (56%), Gaps = 56/608 (9%)
Query: 1 MSGKVIISAVSLILVVGVAIGVVVAVRKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLS 60
M GKV++S S++L+VGVAIGVV + KNG D + Q + ++ +CQ++ D+ C +TL
Sbjct: 1 MIGKVVVSVASILLIVGVAIGVVAFINKNG-DANLSPQMKAVQGICQSTSDKASCVKTLE 59
Query: 61 SVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDLMQF 118
V + DP I A + A D + K+ N + + G K L+ CK + +
Sbjct: 60 PVK---SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 119 ALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKEQFH 178
AL+ L + + ++ + + +I ++ WL V +Y+ C++ ++ +D K I E
Sbjct: 117 ALEDLATIIEEMGED-LSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE-DDLRKAIGE--- 171
Query: 179 VQSLDSVQKVTAVALDIVTGLSDILQQFNLKFD-------------VKPA---------- 215
+ + + +T A+DI + + + N K D P
Sbjct: 172 --GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 229
Query: 216 --SRRLLNSEVTVDDQGYPSWISSSDRKLLAKMQR-----KNWRANIMPNAVVAKDGSGQ 268
+RRLL +D+ G P+W+S +DRKL+AK R + A + N VVAKDGSGQ
Sbjct: 230 GPARRLLED---IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 286
Query: 269 FKTIQAALASYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFA 328
FKT+Q A+ + P+ N+GR +IY+KAG+Y E + +PK NI M+GDG +T+++ +S A
Sbjct: 287 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 346
Query: 329 A--GVKTMQTATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQD 386
G T +AT + GF+ K M F+NTAGP GHQA A R GD + + C GYQD
Sbjct: 347 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 406
Query: 387 SLYVQSNRQYYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNL 445
+LYV + RQ+YRNC+VSGTVDFIFG SAT+IQ++ I+VRK KGQ+NT+TADG++ + +
Sbjct: 407 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 466
Query: 446 NTGIVIQDCNIIPEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGE 505
GIV+Q+C I+P+ L PER T+ +YLGRPWK + TV+M + +GD I P+GW IW GE
Sbjct: 467 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 526
Query: 506 QNHNTCYYAEYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNG 565
H +C Y EY N GPGA RRV W S AE N FTA WL GP W+
Sbjct: 527 SFHKSCRYVEYNNRGPGAFANRRVNWAKV--ARSAAEVNGFTAANWL--GP---INWIQE 579
Query: 566 LHVPHYLG 573
+VP +G
Sbjct: 580 ANVPVTIG 587
>pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilgiana
gi|6714532|dbj|BAA89480.1| pectin methylesterase [Salix
gilgiana]
Length = 596
Score = 416 bits (1070), Expect = e-115
Identities = 244/596 (40%), Positives = 345/596 (56%), Gaps = 50/596 (8%)
Query: 4 KVIISAVSLI---LVVGVAIGVVVAVR------KNGEDPEVQTQQ-----------RNLR 43
K+II++VS I ++VG + +V+ NG P T+ R ++
Sbjct: 24 KIIIASVSSIAFFVIVGAGVFSLVSNHDISSPGNNGGSPSAATRPAEKAKPISHVARVIK 83
Query: 44 IMCQNSQDQKLCHETLSS-VHGAD--AADPKAYIAASVKAATDNVIKAFNMSERLTTEYG 100
+C + Q C TL V G D + PK + ++KAA + + K + +
Sbjct: 84 TVCNATTYQDTCQNTLEKGVLGKDPSSVQPKDLLKIAIKAADEEIDKVIKKASSFKFDKP 143
Query: 101 KENGAKMALNDCKDLMQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACM 160
+E K A +DC +L++ A + L S C+ N+I + D+ NWLSAV+SY+Q C+
Sbjct: 144 RE---KAAFDDCLELIEDAKEELKNSVDCI-GNDIGKLASNAPDLSNWLSAVMSYQQTCI 199
Query: 161 EGFDDANDGEKKIKEQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLL 220
+GF E K+K ++ + +++T+ +L +V+ L L+ F+ + +RRLL
Sbjct: 200 DGFP-----EGKLKSDME-KTFKATRELTSNSLAMVSSLVSFLKNFSFSGTL---NRRLL 250
Query: 221 NSEV---TVDDQGYPSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALA 277
E ++D G P W+S DR++L + + PN VAKDGSG FKTI ALA
Sbjct: 251 AEEQNSPSLDKDGVPGWMSHEDRRILKGADKDKPK----PNVSVAKDGSGDFKTISEALA 306
Query: 278 SYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTA 337
+ P +GRYVI+VK GVYDE +TV K NI MYGDG +TIVTG K+FA GV+T +TA
Sbjct: 307 AMPAKYEGRYVIFVKQGVYDETVTVTKKMANITMYGDGSQKTIVTGNKNFADGVQTFRTA 366
Query: 338 TFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYY 397
TFA GF+ K M F NTAGP+ HQAVA R Q D + + C GYQD+LY Q++RQ+Y
Sbjct: 367 TFAVLGDGFLCKFMGFRNTAGPEKHQAVAIRVQADRAIFLNCRFEGYQDTLYAQTHRQFY 426
Query: 398 RNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNII 457
R+C+++GTVDFIFG + ++ Q+ I VRKP + Q N +TA G + TGIV+Q C I
Sbjct: 427 RSCVITGTVDFIFGDATSVFQNCLITVRKPLENQQNIVTAQGRIDGHETTGIVLQSCRIE 486
Query: 458 PEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYA 517
P+ L P + IRSYLGRPWK ++TV+M+STIGDFIHP GW WQG+ T YYAEY+
Sbjct: 487 PDKDLVPVKNKIRSYLGRPWKEFSRTVIMDSTIGDFIHPGGWLPWQGDFGLKTLYYAEYS 546
Query: 518 NTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
N G GA R+KW GYH +I + EA KFT + Q +W++ P +LG
Sbjct: 547 NKGGGAQTNARIKWPGYH-IIKKEEAMKFTIENFYQ------GDWISASGSPVHLG 595
>gb|AAC27719.1| flower-specific pectin methylesterase precursor [Arabidopsis
thaliana]
Length = 586
Score = 415 bits (1067), Expect = e-114
Identities = 237/606 (39%), Positives = 347/606 (57%), Gaps = 54/606 (8%)
Query: 1 MSGKVIISAVSLILVVGVAIGVVVAVRKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLS 60
M GKV++S S++L+VGVAIGVV + KNG D + Q + ++ +CQ++ D+ C +TL
Sbjct: 1 MIGKVVVSVASILLIVGVAIGVVAFINKNG-DANLSPQMKAVQGICQSTSDKASCVKTLE 59
Query: 61 SVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDLMQF 118
V + DP I A + A D + K+ N + + G K L+ CK + +
Sbjct: 60 PVK---SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 119 ALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKEQFH 178
AL+ L + + ++ + + +I ++ WL V +Y+ C++ ++ +D K I E
Sbjct: 117 ALEDLATIIEEMGED-LSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE-DDLRKAIGE--- 171
Query: 179 VQSLDSVQKVTAVALDIVTGLSDILQQFNLKFD-------------VKPA---------- 215
+ + + +T A+DI + + + N K D P
Sbjct: 172 --GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 229
Query: 216 --SRRLLNSEVTVDDQGYPSWISSSDRKLLAKMQR-----KNWRANIMPNAVVAKDGSGQ 268
+RRLL +D+ G P+W+S +DRKL+AK R + A + N VVAKDGSGQ
Sbjct: 230 GPARRLLED---IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 286
Query: 269 FKTIQAALASYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFA 328
FKT+Q A+ + P+ N+GR +IY+KAG+Y E + +PK NI M+GDG +T+++ +S A
Sbjct: 287 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 346
Query: 329 AGVKTMQTATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSL 388
T + + + GF+ K M F+NTAGP GHQA A R GD + + C GYQD+L
Sbjct: 347 LSRGTTTSLSATVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTL 406
Query: 389 YVQSNRQYYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNLNT 447
YV + RQ+YRNC+VSGTVDFIFG SAT+IQ++ I+VRK KGQ+NT+TADG++ + +
Sbjct: 407 YVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKI 466
Query: 448 GIVIQDCNIIPEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQN 507
GIV+Q+C I+P+ L PER T+ +YLGRPWK + TV+M + +GD I P+GW IW GE
Sbjct: 467 GIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESF 526
Query: 508 HNTCYYAEYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLH 567
H +C Y EY N GPGA RRV W S AE N FTA WL GP W+ +
Sbjct: 527 HKSCRYVEYNNRGPGAFANRRVNWAKV--ARSAAEVNGFTAANWL--GP---INWIQEAN 579
Query: 568 VPHYLG 573
VP +G
Sbjct: 580 VPVTIG 585
>gb|AAV91509.1| VGD1-like protein 1 [Arabidopsis thaliana]
Length = 588
Score = 412 bits (1058), Expect = e-113
Identities = 237/608 (38%), Positives = 348/608 (56%), Gaps = 56/608 (9%)
Query: 1 MSGKVIISAVSLILVVGVAIGVVVAVRKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLS 60
M GKV++S S++L+VGVAIGVV + KNG D + Q + ++ +C+++ D+ C +TL
Sbjct: 1 MIGKVVVSVASILLIVGVAIGVVAFINKNG-DANLSPQMKAVQGICRSTSDKASCVKTLE 59
Query: 61 SVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDLMQF 118
V + DP I A + A D + K+ N + + G K L+ CK + +
Sbjct: 60 PVK---SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRVFMY 116
Query: 119 ALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKEQFH 178
AL+ L + + ++ + + +I ++ WL V +Y+ C++ ++ +D K I E
Sbjct: 117 ALEDLATIIEEMGED-LSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE-DDLRKAIGE--- 171
Query: 179 VQSLDSVQKVTAVALDIVTGLSDILQQFNLKFD-------------VKPA---------- 215
+ + + +T A+DI + + + N K D P
Sbjct: 172 --GIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 229
Query: 216 --SRRLLNSEVTVDDQGYPSWISSSDRKLLAKMQR-----KNWRANIMPNAVVAKDGSGQ 268
+RRLL +D+ G P+W+S +DRKL+AK R + A + N VVAKDGSGQ
Sbjct: 230 GPARRLLED---IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRANFVVAKDGSGQ 286
Query: 269 FKTIQAALASYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFA 328
FKT+Q A+ + P+ N+GR +IY+KAG+Y E + +PK NI M+GDG +T+++ +S A
Sbjct: 287 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKNNNIFMFGDGARKTVISYNRSVA 346
Query: 329 A--GVKTMQTATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQD 386
G T +AT + GF+ K M F+NTAGP GHQA A R GD + + C GYQD
Sbjct: 347 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 406
Query: 387 SLYVQSNRQYYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNL 445
+LYV + RQ+YRNC+VSGTVDFIFG SAT+IQ++ I+VRK KGQ+NT+TADG++ + +
Sbjct: 407 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 466
Query: 446 NTGIVIQDCNIIPEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGE 505
GIV+Q+C I+P+ L PER T+ +YLGRPWK + TV+M + +GD I P+GW IW GE
Sbjct: 467 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 526
Query: 506 QNHNTCYYAEYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNG 565
H +C Y EY N GPGA RR W S AE + FTA WL GP W+
Sbjct: 527 SFHKSCRYVEYNNRGPGAFANRRANWAKV--ARSAAEVSGFTAANWL--GP---INWIQE 579
Query: 566 LHVPHYLG 573
+VP +G
Sbjct: 580 ANVPVTIG 587
>emb|CAA39658.1| Bp19 [Brassica napus] gi|99823|pir||S14952 pectinesterase homolog -
rape gi|1172538|sp|P41510|PME_BRANA Probable
pectinesterase precursor (Pectin methylesterase) (PE)
Length = 584
Score = 391 bits (1005), Expect = e-107
Identities = 236/599 (39%), Positives = 337/599 (55%), Gaps = 47/599 (7%)
Query: 3 GKVIISAVSLILVVGVAIGVVVAVRKNGE---DPEVQTQQRNLRIMCQNSQDQKLCHETL 59
GK++IS S++LVVGVAIGVV V K G D + + Q+ + +C ++ D+ C +TL
Sbjct: 4 GKIVISVASMLLVVGVAIGVVTFVNKGGGAGGDKTLNSHQKAVESLCASATDKGSCAKTL 63
Query: 60 SSVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDLMQ 117
V + DP I A + A D V K+ N + GK A K L+ CK ++
Sbjct: 64 DPVK---SDDPSKLIKAFMLATKDAVTKSTNFTASTEEGMGKNINATSKAVLDYCKRVLM 120
Query: 118 FALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKEQF 177
+AL+ L+ + + ++ +Q ++ ++ WL+ V +Y+ C+ DD + E + +
Sbjct: 121 YALEDLETIVEEMGED-LQQSGSKMDQLKQWLTGVFNYQTDCI---DDIEESELR---KV 173
Query: 178 HVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFD--------VKPASRRLLNSEVTVDDQ 229
+ + + +++ A+DI L+ + Q N+K D PA R L ++ D +
Sbjct: 174 MGEGIAHSKILSSNAIDIFHALTTAMSQMNVKVDDMKKGNLGETPAPDRDLLEDL--DQK 231
Query: 230 GYPSWISSSDRKLLAKMQRKNWRAN------------IMPNAVVAKDGSGQFKTIQAALA 277
G P W S DRKL+A+ R A+ I P VVAKDGSGQFKTI A+
Sbjct: 232 GLPKWHSDKDRKLMAQAGRPGAPADEGIGEGGGGGGKIKPTHVVAKDGSGQFKTISEAVK 291
Query: 278 SYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKS--FAAGVKTMQ 335
+ P+ N GR +IY+KAGVY E +T+PK N+ M+GDG +TI+T +S + G T
Sbjct: 292 ACPEKNPGRCIIYIKAGVYKEQVTIPKKVNNVFMFGDGATQTIITFDRSVGLSPGTTTSL 351
Query: 336 TATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQ 395
+ T + GF+ K + F+NTAGP GHQAVAFR GD + + C GYQD+LYV + RQ
Sbjct: 352 SGTVQVESEGFMAKWIGFQNTAGPLGHQAVAFRVNGDRAVIFNCRFDGYQDTLYVNNGRQ 411
Query: 396 YYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNLNTGIVIQDC 454
+YRN +VSGTVDFIFG SAT+IQ+S I+ RK GQ N +TADG++ + GIV+ +C
Sbjct: 412 FYRNIVVSGTVDFIFGKSATVIQNSLILCRKGSPGQTNHVTADGNEKGKAVKIGIVLHNC 471
Query: 455 NIIPEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYA 514
I+ + L +R T++SYLGRPWK A T V+ + IGD I P GW WQGE+ H T Y
Sbjct: 472 RIMADKELEADRLTVKSYLGRPWKPFATTAVIGTEIGDLIQPTGWNEWQGEKFHLTATYV 531
Query: 515 EYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
E+ N GPGAN A RV W S AE +FT WL + A W+ +VP LG
Sbjct: 532 EFNNRGPGANTAARVPWAKM--AKSAAEVERFTVANWL-----TPANWIQEANVPVQLG 583
>emb|CAB71877.1| PECTINESTERASE-like protein [Arabidopsis thaliana]
gi|21536813|gb|AAM61145.1| PECTINESTERASE-like protein
[Arabidopsis thaliana] gi|20260676|gb|AAM13236.1|
pectinesterase-like protein [Arabidopsis thaliana]
gi|61213924|sp|Q5MFV6|PMEL_ARATH Probable pectinesterase
VGDH2 precursor (Pectin methylesterase) (PE)
(VANGUARD1-like protein 2) (VGD1-like protein 2)
gi|15228697|ref|NP_191776.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 588
Score = 390 bits (1001), Expect = e-107
Identities = 234/604 (38%), Positives = 336/604 (54%), Gaps = 53/604 (8%)
Query: 3 GKVIISAVSLILVVGVAIGVVVAVRKNG-----EDPEVQTQQRNLRIMCQNSQDQKLCHE 57
GKV++S SL+LVVGVAIGV+ V K G + + + Q+ ++ +CQ++ DQ C +
Sbjct: 4 GKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQGSCAK 63
Query: 58 TLSSVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDL 115
TL V + DP + A + A D + K+ N + G A K L+ CK +
Sbjct: 64 TLDPVK---SDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRV 120
Query: 116 MQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKE 175
+ +AL+ L+ + + ++ +Q ++ ++ WL+ V +Y+ C+ DD + E K
Sbjct: 121 LMYALEDLETIVEEMGED-LQQSGTKLDQLKQWLTGVFNYQTDCL---DDIEEVELK--- 173
Query: 176 QFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFD----------VKPASRRLLNSEVT 225
+ + + + + +T+ A+DI + + Q +K D A+RRLL
Sbjct: 174 KIMGEGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDN-- 231
Query: 226 VDDQGYPSWISSSDRKLLAKMQRK-------------NWRANIMPNAVVAKDGSGQFKTI 272
D +G P W S DRKL+AK R I VVAKDGSGQFKTI
Sbjct: 232 -DSKGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTI 290
Query: 273 QAALASYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKS--FAAG 330
A+ + P N GR +I++KAG+Y+E + +PK NI M+GDG +TI+T +S + G
Sbjct: 291 SEAVMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPG 350
Query: 331 VKTMQTATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYV 390
T + T + GF+ K + F+NTAGP GHQAVA R GD + + C GYQD+LYV
Sbjct: 351 TTTSLSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYV 410
Query: 391 QSNRQYYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNLNTGI 449
+ RQ+YRN +VSGTVDFIFG SAT+IQ+S I+VRK GQ N +TADG++ + GI
Sbjct: 411 NNGRQFYRNIVVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNYVTADGNEKGAAMKIGI 470
Query: 450 VIQDCNIIPEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHN 509
V+ +C IIP+ L ++ TI+SYLGRPWK A TV++ + IGD I P+GWT WQGEQNH
Sbjct: 471 VLHNCRIIPDKELEADKLTIKSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQNHK 530
Query: 510 TCYYAEYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVP 569
T Y E+ N GPGA +R W S AE +T W+ GP A W+ +VP
Sbjct: 531 TAKYIEFNNRGPGAATTQRPPW--VKVAKSAAEVETYTVANWV--GP---ANWIQEANVP 583
Query: 570 HYLG 573
LG
Sbjct: 584 VQLG 587
>gb|AAV91510.1| VGD1-like protein 2 [Arabidopsis thaliana]
Length = 588
Score = 385 bits (989), Expect = e-105
Identities = 232/604 (38%), Positives = 334/604 (54%), Gaps = 53/604 (8%)
Query: 3 GKVIISAVSLILVVGVAIGVVVAVRKNG-----EDPEVQTQQRNLRIMCQNSQDQKLCHE 57
GKV++S SL+LVVGVAIGV+ V K G + + + Q+ ++ +C ++ DQ C +
Sbjct: 4 GKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICLSTTDQGSCAK 63
Query: 58 TLSSVHGADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDL 115
TL V + DP + A + A D + K+ N + G A K L+ CK +
Sbjct: 64 TLDPVK---SDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRV 120
Query: 116 MQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKE 175
+ +AL+ L+ + + ++ +Q ++ ++ WL+ V +Y+ C+ ++ KKI
Sbjct: 121 LMYALEDLETIVEEMGED-LQQSGTKLDQLKQWLTGVFNYQTDCLGDIEEVE--LKKIMG 177
Query: 176 QFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFD----------VKPASRRLLNSEVT 225
+ + + + +T+ A+DI + + Q +K D A+RRLL
Sbjct: 178 E----GISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDN-- 231
Query: 226 VDDQGYPSWISSSDRKLLAKMQRK-------------NWRANIMPNAVVAKDGSGQFKTI 272
D +G P W S DRKL+AK R I VVAKDGSGQFKTI
Sbjct: 232 -DSKGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTI 290
Query: 273 QAALASYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKS--FAAG 330
A+ + P N GR +I++KAG+Y+E + +PK NI M+GDG +TI+T +S + G
Sbjct: 291 SEAVMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITFDRSVKLSPG 350
Query: 331 VKTMQTATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYV 390
T + T + GF+ K + F+NTAGP GHQAVA R GD + + C GYQD+LYV
Sbjct: 351 TTTSLSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTLYV 410
Query: 391 QSNRQYYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNLNTGI 449
+ RQ+YRN +VSGTVDFIFG SAT+IQ+S I+VRK GQ N +TADG++ + GI
Sbjct: 411 NNGRQFYRNIVVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNYVTADGNEKGAAMKIGI 470
Query: 450 VIQDCNIIPEAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHN 509
V+ +C IIP+ L ++ TI+SYLGRPWK A TV++ + IGD I P+GWT WQGEQNH
Sbjct: 471 VLHNCRIIPDKELEADKLTIKSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQNHK 530
Query: 510 TCYYAEYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVP 569
T Y E+ N GPGA +R W S AE +T W+ GP A W+ +VP
Sbjct: 531 TAKYIEFNNRGPGAATTQRPPW--VKVAKSAAEVETYTVANWV--GP---ANWIQEANVP 583
Query: 570 HYLG 573
LG
Sbjct: 584 VQLG 587
>ref|XP_474065.1| OSJNBb0079B02.7 [Oryza sativa (japonica cultivar-group)]
gi|32490042|emb|CAE05961.1| OSJNBa0063C18.2 [Oryza
sativa (japonica cultivar-group)]
gi|38344904|emb|CAE02974.2| OSJNBb0079B02.7 [Oryza
sativa (japonica cultivar-group)]
Length = 971
Score = 371 bits (952), Expect = e-101
Identities = 185/344 (53%), Positives = 230/344 (66%), Gaps = 13/344 (3%)
Query: 231 YPSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIY 290
+PSW+S+ R+LL +K P+ VVAKDGSG FKTI A+ + PK + R+VIY
Sbjct: 639 FPSWVSAHQRRLLQAGTQK-------PDKVVAKDGSGDFKTITEAVNAVPKNSPTRFVIY 691
Query: 291 VKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKA 350
VKAG Y+EY+T+P NI MYGDGP +T V G KS GV TM T TF+ GF+ K+
Sbjct: 692 VKAGEYNEYVTIPSSLPNIFMYGDGPTKTRVLGNKSNKDGVATMATRTFSAEGNGFVCKS 751
Query: 351 MTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIF 410
M F NTAGP+GHQAVA QGDMS C GYQD+LYV +NRQ++RNC V+GT+D+IF
Sbjct: 752 MGFVNTAGPEGHQAVALHVQGDMSVFFNCKFEGYQDTLYVHANRQFFRNCEVTGTIDYIF 811
Query: 411 GSSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIR 470
G+SA + Q + VRKP Q N +TA G N+ TGIV+QDC I+PE ALFP R I
Sbjct: 812 GNSAAVFQSCLMTVRKPMDNQANMVTAHGRTDPNMPTGIVLQDCRIVPEQALFPVRLQIA 871
Query: 471 SYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVK 530
SYLGRPWK A+TVVMES IGDFI P+GW+ W G+ T YYAEYANTGPGA ++RV
Sbjct: 872 SYLGRPWKEYARTVVMESVIGDFIKPEGWSEWMGDVGLKTLYYAEYANTGPGAGTSKRVT 931
Query: 531 WKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLGF 574
W GY VI +AEA +FTAG+++ WL P+ +GF
Sbjct: 932 WPGYR-VIGQAEATQFTAGVFI-----DGLTWLKNTATPNVMGF 969
Score = 68.6 bits (166), Expect = 5e-10
Identities = 58/203 (28%), Positives = 106/203 (51%), Gaps = 25/203 (12%)
Query: 6 IISAVSLILVVGVAIGVVVAVRK--NGEDPEVQTQQRNLRIMCQNSQDQKLCHETLSSVH 63
I++AV ++ VV AI V + +K NGE T L +C ++ C ++L V
Sbjct: 10 IVAAVGVVAVV-CAIAAVTSSKKDRNGE----LTANVRLSTVCSVTRYPGRCEQSLGPVV 64
Query: 64 GADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKM---ALNDCKDLMQFAL 120
D DP++ + A+++ A + V AFN S + GK++ AK+ A+ CK L+ A+
Sbjct: 65 N-DTIDPESVLRAALQVALEEVTSAFNRS----MDVGKDDDAKITKSAIEMCKKLLDDAI 119
Query: 121 DSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKEQFHVQ 180
+ L + + + V + D+R WLS+V++Y C +GFD + ++KE +
Sbjct: 120 EDL----RGMASLKPEEVTKHVNDLRCWLSSVMTYIYTCADGFD-----KPELKEAMD-K 169
Query: 181 SLDSVQKVTAVALDIVTGLSDIL 203
L + ++++ AL I+T L +++
Sbjct: 170 LLQNSTELSSNALAIITSLGELM 192
>emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (indica
cultivar-group)]
Length = 717
Score = 371 bits (952), Expect = e-101
Identities = 185/344 (53%), Positives = 230/344 (66%), Gaps = 13/344 (3%)
Query: 231 YPSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIY 290
+PSW+S+ R+LL +K P+ VVAKDGSG FKTI A+ + PK + R+VIY
Sbjct: 385 FPSWVSAHQRRLLQAGTQK-------PDKVVAKDGSGDFKTITEAVNAVPKNSPTRFVIY 437
Query: 291 VKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKA 350
VKAG Y+EY+T+P NI MYGDGP +T V G KS GV TM T TF+ GF+ K+
Sbjct: 438 VKAGEYNEYVTIPSSLPNIFMYGDGPTKTRVLGNKSNKDGVATMATRTFSAEGNGFVCKS 497
Query: 351 MTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIF 410
M F NTAGP+GHQAVA QGDMS C GYQD+LYV +NRQ++RNC V+GT+D+IF
Sbjct: 498 MGFVNTAGPEGHQAVALHVQGDMSVFFNCKFEGYQDTLYVHANRQFFRNCEVTGTIDYIF 557
Query: 411 GSSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIR 470
G+SA + Q + VRKP Q N +TA G N+ TGIV+QDC I+PE ALFP R I
Sbjct: 558 GNSAAVFQSCLMTVRKPMDNQANMVTAHGRTDPNMPTGIVLQDCRIVPEQALFPVRLQIA 617
Query: 471 SYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVK 530
SYLGRPWK A+TVVMES IGDFI P+GW+ W G+ T YYAEYANTGPGA ++RV
Sbjct: 618 SYLGRPWKEYARTVVMESVIGDFIKPEGWSEWMGDVGLKTLYYAEYANTGPGAGTSKRVT 677
Query: 531 WKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLGF 574
W GY VI +AEA +FTAG+++ WL P+ +GF
Sbjct: 678 WPGYR-VIGQAEATQFTAGVFI-----DGLTWLKNTATPNVMGF 715
>pir||T52325 pectinesterase (EC 3.1.1.11) [imported] - turnip (fragment)
gi|1448939|gb|AAB04617.1| pectinesterase
gi|6093741|sp|Q42608|PME_BRACM Pectinesterase (Pectin
methylesterase) (PE)
Length = 571
Score = 369 bits (946), Expect = e-100
Identities = 228/589 (38%), Positives = 325/589 (54%), Gaps = 47/589 (7%)
Query: 13 ILVVGVAIGVVVAVRKNGE---DPEVQTQQRNLRIMCQNSQDQKLCHETLSSVHGADAAD 69
+LVVGVAIGVV V K G D + + Q+ + +C ++ D+ C +TL V + D
Sbjct: 1 LLVVGVAIGVVTFVNKGGGAGGDKTLNSHQKAVESLCASATDKGSCAKTLDPVK---SDD 57
Query: 70 PKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGA--KMALNDCKDLMQFALDSLDLST 127
P I A + A D V K+ N + GK A K L+ CK ++ +AL+ L+
Sbjct: 58 PSKLIKAFMLATKDAVTKSTNFTASTEEGMGKNMNATSKAVLDYCKRVLMYALEDLETIV 117
Query: 128 KCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKIKEQFHVQSLDSVQK 187
+ + ++ +Q ++ ++ WL+ V +Y+ C+ DD + E + + + + +
Sbjct: 118 EEMGED-LQQSGSKMDQLKQWLTGVFNYQTDCI---DDIEESELR---KVMGEGIRHSKI 170
Query: 188 VTAVALDIVTGLSDILQQFNLKFD--------VKPASRRLLNSEVTVDDQGYPSWISSSD 239
+++ A+DI L+ + Q N+K D PA R L ++ D +G P W S D
Sbjct: 171 LSSNAIDIFHALTTAMSQMNVKVDDMKKGNLGETPAPDRDLLEDL--DQKGLPKWHSDKD 228
Query: 240 RKLLAKMQRKNWRAN------------IMPNAVVAKDGSGQFKTIQAALASYPKGNKGRY 287
RKL+A+ R A+ I P VVAKDGSGQFKTI A+ + P+ N GR
Sbjct: 229 RKLMAQAGRPGAPADEGIGEGGGGGGKIKPTHVVAKDGSGQFKTISEAVKACPEKNPGRC 288
Query: 288 VIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKS--FAAGVKTMQTATFANTAMG 345
+IY+KAGVY E +T+PK N+ M+GDG +TI+T +S + G T + T + G
Sbjct: 289 IIYIKAGVYKEQVTIPKKVNNVFMFGDGATQTIITFDRSVGLSPGTTTSLSGTVQVESEG 348
Query: 346 FIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGT 405
F+ K + F+NTAGP G+QAVAFR GD + + C GYQD+LYV + RQ+YRN +VSGT
Sbjct: 349 FMAKWIGFQNTAGPLGNQAVAFRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNIVVSGT 408
Query: 406 VDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDT-MNLNTGIVIQDCNIIPEAALFP 464
VDFI G SAT+IQ+S I+ RK GQ N +TADG + GIV+ +C I+ + L
Sbjct: 409 VDFINGKSATVIQNSLILCRKGSPGQTNHVTADGKQKGKAVKIGIVLHNCRIMADKELEA 468
Query: 465 ERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGAN 524
+R T++SYLGRPWK A T V+ + IGD I P GW WQGE+ H T Y E+ N GPGAN
Sbjct: 469 DRLTVKSYLGRPWKPFATTAVIGTEIGDLIQPTGWNEWQGEKFHLTATYVEFNNRGPGAN 528
Query: 525 VARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
A RV W S AE +FT WL + A W+ +V LG
Sbjct: 529 PAARVPWAKM--AKSAAEVERFTVANWL-----TPANWIQEANVTVQLG 570
>gb|AAB57670.1| pectinesterase [Citrus sinensis]
Length = 584
Score = 366 bits (940), Expect = e-100
Identities = 213/577 (36%), Positives = 320/577 (54%), Gaps = 29/577 (5%)
Query: 4 KVIISAVSLILVVGVAIGVVVAV--RKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLSS 61
K+ ++ + +LVV IG+V V RKN D + L+ C +++ LC +++
Sbjct: 29 KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88
Query: 62 VHGAD--AADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALNDCKDLMQFA 119
V A K I S+ T V + ++L K+AL+DC + +
Sbjct: 89 VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDET 148
Query: 120 LDSLDLSTKCVHDN-NIQAVHDQIADMRNWLSAVISYRQACMEGF--DDANDGEKKIKEQ 176
LD L + + + + N +++ D++ +SA ++ + C++GF DDAN +
Sbjct: 149 LDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSD 208
Query: 177 FHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLLNSEVTVDDQGYPSWIS 236
V V+K+ + AL ++ ++D ++ ++ R L E + D G+P+W+S
Sbjct: 209 GQVH----VEKMCSNALAMIKNMTDTDMMI-----MRTSNNRKLTEETSTVD-GWPAWLS 258
Query: 237 SSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIYVKAGVY 296
DR+LL +++ PNAVVA DGSG FKT+ AA+A+ P+G RY+I +KAGVY
Sbjct: 259 PGDRRLLQS-------SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVY 311
Query: 297 DEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKAMTFENT 356
E + V K NI+ GDG RTI+TG ++ G T ++AT A GF+ + +TF+NT
Sbjct: 312 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 371
Query: 357 AGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFGSSATL 416
AGP HQAVA R D+SA C ++ YQD+LYV SNRQ++ NCL++GTVDFIFG++A +
Sbjct: 372 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 431
Query: 417 IQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIRSYLGRP 476
+Q+ I RKP GQ N +TA G N NTGIVIQ I + L P + + +YLGRP
Sbjct: 432 LQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 491
Query: 477 WKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVKWKGYHG 536
WK ++TV+M+S+I D IHP GW W G NT +Y E+ N+G GA + RVKWKG+
Sbjct: 492 WKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRV 551
Query: 537 VISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
+ S EA FT G ++ + + WL P LG
Sbjct: 552 ITSATEAQAFTPGSFI-----AGSSWLGSTGFPFSLG 583
>sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methylesterase) (PE)
gi|2098705|gb|AAB57667.1| pectinesterase [Citrus
sinensis]
Length = 584
Score = 366 bits (939), Expect = 1e-99
Identities = 213/577 (36%), Positives = 320/577 (54%), Gaps = 29/577 (5%)
Query: 4 KVIISAVSLILVVGVAIGVVVAV--RKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLSS 61
K+ ++ + +LVV IG+V V RKN D + L+ C +++ LC +++
Sbjct: 29 KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88
Query: 62 VHGAD--AADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALNDCKDLMQFA 119
V A K I S+ T V + ++L K+AL+DC + +
Sbjct: 89 VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDET 148
Query: 120 LDSLDLSTKCVHDN-NIQAVHDQIADMRNWLSAVISYRQACMEGF--DDANDGEKKIKEQ 176
LD L + + + + N +++ D++ +SA ++ + C++GF DDAN +
Sbjct: 149 LDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSD 208
Query: 177 FHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLLNSEVTVDDQGYPSWIS 236
V V+K+ + AL ++ ++D ++ ++ R L E + D G+P+W+S
Sbjct: 209 GQVH----VEKMCSNALAMIKNMTDTDMMI-----MRTSNNRKLTEETSTVD-GWPAWLS 258
Query: 237 SSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIYVKAGVY 296
DR+LL +++ PNAVVA DGSG FKT+ AA+A+ P+G RY+I +KAGVY
Sbjct: 259 PGDRRLLQS-------SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVY 311
Query: 297 DEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKAMTFENT 356
E + V K NI+ GDG RTI+TG ++ G T ++AT A GF+ + +TF+NT
Sbjct: 312 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNT 371
Query: 357 AGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFGSSATL 416
AGP HQAVA R D+SA C ++ YQD+LYV SNRQ++ NCL++GTVDFIFG++A +
Sbjct: 372 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 431
Query: 417 IQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIRSYLGRP 476
+Q+ I RKP GQ N +TA G N NTGIVIQ I + L P + + +YLGRP
Sbjct: 432 LQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 491
Query: 477 WKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVKWKGYHG 536
WK ++TV+M+S+I D IHP GW W G NT +Y E+ N+G GA + RVKWKG+
Sbjct: 492 WKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRV 551
Query: 537 VISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
+ S EA FT G ++ + + WL P LG
Sbjct: 552 ITSATEAQAFTPGSFI-----AGSSWLGSTGFPFSLG 583
>emb|CAB80040.1| pectinesterase-like protein [Arabidopsis thaliana]
gi|4455337|emb|CAB36797.1| pectinesterase-like protein
[Arabidopsis thaliana] gi|15234112|ref|NP_195049.1|
pectinesterase family protein [Arabidopsis thaliana]
gi|7447390|pir||T05203 pectinesterase homolog F4I10.160
- Arabidopsis thaliana
Length = 609
Score = 365 bits (937), Expect = 2e-99
Identities = 210/536 (39%), Positives = 306/536 (56%), Gaps = 29/536 (5%)
Query: 42 LRIMCQNSQDQKLCHETLSSVHGADA--ADPKAYIAASVKAATDNVIKAFNMSERLTTEY 99
++ +C ++ + C TL + D DP++ + +++ A D++ + F L TE
Sbjct: 95 IQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTEN 154
Query: 100 GKENGAKMALNDCKDLMQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQAC 159
+ K A+ CK L+ A + L S K ++D+ + + D+ +WLSAV+SY++ C
Sbjct: 155 KDD---KDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQETC 211
Query: 160 MEGFDDANDGEKKIKEQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRL 219
++GF+ E K+K + ++ +S Q +T+ +L ++ L L K +R L
Sbjct: 212 VDGFE-----EGKLKTEIR-KNFNSSQVLTSNSLAMIKSLDGYLSSVP-----KVKTRLL 260
Query: 220 LNSEVTVDDQGY-PSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALAS 278
L + + + + SW+S+ +R++L + K + PNA VAKDGSG F TI AAL +
Sbjct: 261 LEARSSAKETDHITSWLSNKERRMLKAVDVKA----LKPNATVAKDGSGNFTTINAALKA 316
Query: 279 YPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTAT 338
P +GRY IY+K G+YDE + + K N+ M GDG +TIVTG KS A ++T TAT
Sbjct: 317 MPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTAT 376
Query: 339 FANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYR 398
F GF+ ++M F NTAGP+GHQAVA R Q D S + C GYQD+LY ++RQYYR
Sbjct: 377 FVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYR 436
Query: 399 NCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIP 458
+C++ GTVDFIFG +A + Q+ I +RK GQ NT+TA G TG VI +C + P
Sbjct: 437 SCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAP 496
Query: 459 EAALFPERFTIRSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQ-GEQNHNTCYYAEYA 517
L P + +SYLGRPWK ++TVVMESTI D I P GW WQ + +T YAEY
Sbjct: 497 NEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYK 556
Query: 518 NTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
N GP A RVKW G+ V+++ EA KFT G +LQ EW+ + P LG
Sbjct: 557 NDGPSGATAARVKWPGFR-VLNKEEAMKFTVGPFLQ------GEWIQAIGSPVKLG 605
>sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methylesterase 3) (PE 3)
Length = 584
Score = 364 bits (934), Expect = 5e-99
Identities = 212/577 (36%), Positives = 320/577 (54%), Gaps = 29/577 (5%)
Query: 4 KVIISAVSLILVVGVAIGVVVAV--RKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLSS 61
K+ ++ + +LVV IG+V V RKN D + L+ C +++ LC +++
Sbjct: 29 KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88
Query: 62 VHGAD--AADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALNDCKDLMQFA 119
V A K I S+ T V + ++L K+AL+DC + +
Sbjct: 89 VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDET 148
Query: 120 LDSLDLSTKCVHDN-NIQAVHDQIADMRNWLSAVISYRQACMEGF--DDANDGEKKIKEQ 176
LD L + + + + N +++ D++ +SA ++ + C++GF DDAN +
Sbjct: 149 LDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSD 208
Query: 177 FHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLLNSEVTVDDQGYPSWIS 236
V V+K+ + AL ++ ++D ++ +R+L+ TVD G+P+W+S
Sbjct: 209 GQVH----VEKMCSNALAMIKNMTDT----DMMIMRTSNNRKLIEETSTVD--GWPAWLS 258
Query: 237 SSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIYVKAGVY 296
+ DR+LL +++ PN VVA DGSG FKT+ A++A+ P+G RY+I +KAGVY
Sbjct: 259 TGDRRLLQS-------SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVY 311
Query: 297 DEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKAMTFENT 356
E + V K NI+ GDG RTI+TG ++ G T ++AT A GF+ + +TF+NT
Sbjct: 312 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 371
Query: 357 AGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFGSSATL 416
AGP HQAVA R D+SA C ++ YQD+LYV SNRQ++ NCL++GTVDFIFG++A +
Sbjct: 372 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 431
Query: 417 IQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIRSYLGRP 476
+Q+ I RKP GQ N +TA G N NTGIVIQ I + L P + + +YLGRP
Sbjct: 432 LQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 491
Query: 477 WKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVKWKGYHG 536
WK ++TV+M+S+I D IHP GW W G NT +Y E+ N G GA + RVKWKG+
Sbjct: 492 WKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRV 551
Query: 537 VISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
+ S EA FT G ++ + + WL P LG
Sbjct: 552 ITSATEAQAFTPGSFI-----AGSSWLGSTGFPFSLG 583
>emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]
Length = 579
Score = 362 bits (929), Expect = 2e-98
Identities = 215/582 (36%), Positives = 320/582 (54%), Gaps = 31/582 (5%)
Query: 4 KVIISAVSLILVVGVAIGVVVAV--RKNGEDPEVQTQQRN------LRIMCQNSQDQKLC 55
K+ ++ V+ +L+V IGVVV V R N D Q ++ C+N+ +LC
Sbjct: 16 KLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSAAHAIVKSACENTLHPELC 75
Query: 56 HETLSSVH--GADAADPKAYIAASVKAATDNVIKAFNMSERLT-TEYGKENGAKMALNDC 112
+ T++SV K I S+ V F E+L T G + K+AL+DC
Sbjct: 76 YSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVEKLIKTRKGLKPREKVALHDC 135
Query: 113 KDLMQFALDSLDLSTKCVH-DNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEK 171
+ + LD L + K + N +++ D++ +S+ I+ ++ C++GF +D +K
Sbjct: 136 LETIDETLDELHTAIKDLELYPNKKSLKAHADDLKTLISSAITNQETCLDGFSH-DDADK 194
Query: 172 KIKEQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLLNSEVTVDDQGY 231
K+++ ++ V+K+ + AL ++ ++D K +R+L D+ +
Sbjct: 195 KVRKAL-LKGQKHVEKMCSNALAMICNMTDTDIANEQKLKGTTTNRKLRE-----DNSEW 248
Query: 232 PSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIYV 291
P W+S+ DR+LL + + P+ VVA DGSG FKT+ A+A P+ + RYVI +
Sbjct: 249 PEWLSAGDRRLLQS-------STVRPDVVVAADGSGNFKTVSEAVAKAPEKSSKRYVIRI 301
Query: 292 KAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKAM 351
KAGVY E + VPK NI+ GDG + TI+TG ++ G T +AT A F+ + +
Sbjct: 302 KAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVGEKFLARDI 361
Query: 352 TFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFG 411
TF+NTAG HQAVA R D+SA C I+ YQDSLYV SNRQY+ CL++GTVDFIFG
Sbjct: 362 TFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGTVDFIFG 421
Query: 412 SSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIRS 471
++A ++Q+ I R+PG GQ N +TA G N NTGIVIQ C I + L P + + +
Sbjct: 422 NAAAVLQNCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQKSFPT 481
Query: 472 YLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVKW 531
YLGRPWK ++TV+M+S+I D I+ GW W G NT +Y EY NTG GA + RVKW
Sbjct: 482 YLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGTSGRVKW 541
Query: 532 KGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
KG+ + S EA +T G ++ G WL+ P LG
Sbjct: 542 KGFKVITSATEAQAYTPGRFIAGG-----SWLSSTGFPFSLG 578
>ref|NP_198139.1| pectinesterase family protein [Arabidopsis thaliana]
Length = 732
Score = 360 bits (925), Expect = 5e-98
Identities = 212/572 (37%), Positives = 314/572 (54%), Gaps = 32/572 (5%)
Query: 5 VIISAVSLI-LVVGVAIGVVVAVRKNGEDPEVQTQQRNLRIMCQNSQDQKLCHETLSSVH 63
+ IS+V LI +VV V IGV V N D E+ T + ++ +C + ++ C +TL
Sbjct: 19 ISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRK-D 77
Query: 64 GADAADPKAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALNDCKDLMQFALDSL 123
D +DP + + A + S+ + E K+ AKMAL+ CK+LM +A+ L
Sbjct: 78 AKDTSDPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDYAIGEL 136
Query: 124 DLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFD--DANDGEKKIKEQFHVQS 181
S + + V + + +R WLSA IS+ Q C++GF N GE K +
Sbjct: 137 SKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKK------A 190
Query: 182 LDSVQKVTAVALDIVTGLSDILQQFNLKFDVKPASRRLLNSEVTVDDQGYPSWISSSDRK 241
L + ++T L +VT +S+ L Q + + SRRLL+ E +PSW+ + R+
Sbjct: 191 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLSQE-------FPSWMDARARR 240
Query: 242 LLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIYVKAGVYDEYIT 301
LL + + P+ VVA+DGSGQ+KTI AL PK +V+++K G+Y EY+
Sbjct: 241 LLNAPM-----SEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQ 295
Query: 302 VPKDAVNILMYGDGPARTIVTGRKSFAAGVKTMQTATFANTAMGFIGKAMTFENTAGPDG 361
V + +++ GDGP +T+++G KS+ G+ T +TAT A FI K + FENTAG
Sbjct: 296 VNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIK 355
Query: 362 HQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFGSSATLIQHST 421
HQAVA R D S C GYQD+LY S+RQ+YR+C +SGT+DF+FG +A + Q+ T
Sbjct: 356 HQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCT 415
Query: 422 IIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTIRSYLGRPWKYLA 481
++VRKP Q ITA G +TG V+Q C I+ E + ++YLGRPWK +
Sbjct: 416 LLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYS 475
Query: 482 KTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRVKWKGYHGVISRA 541
+T++M + I DF+ P+GW W GE NT +Y+E NTGPGA + +RV W G +S
Sbjct: 476 RTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIK-KLSDE 534
Query: 542 EANKFTAGIWLQAGPKSAAEWLNGLHVPHYLG 573
E KFT ++Q W+ G VP+ LG
Sbjct: 535 EILKFTPAQYIQGD-----AWIPGKGVPYILG 561
>gb|AAM65978.1| pectin methylesterase [Arabidopsis thaliana]
gi|10177147|dbj|BAB10336.1| pectin methylesterase
[Arabidopsis thaliana] gi|18700151|gb|AAL77687.1|
AT5g49180/K21P3_5 [Arabidopsis thaliana]
gi|15239814|ref|NP_199729.1| pectinesterase family
protein [Arabidopsis thaliana]
gi|25090082|gb|AAN72223.1| At5g49180/K21P3_5
[Arabidopsis thaliana]
Length = 571
Score = 360 bits (923), Expect = 9e-98
Identities = 214/586 (36%), Positives = 322/586 (54%), Gaps = 39/586 (6%)
Query: 4 KVIISAVSLILVVGVAIGVVVAVRKNGEDPE------VQTQQRNLRIMCQNSQDQKLCHE 57
K II+ V L+V + + V + +N E ++T + +C + ++ C
Sbjct: 11 KCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVN 70
Query: 58 TLSSVHGADAADP----KAYIAASVKAATDNVIKAFNMSERLTTEYGKENGAKMALNDCK 113
+L D+ P K ++++ D++ KA S LT + + K AL C+
Sbjct: 71 SLMKA-SPDSTQPLDLIKLGFNVTIRSIEDSIKKA---SVELTAKAANDKDTKGALELCE 126
Query: 114 DLMQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWLSAVISYRQACMEGFDDANDGEKKI 173
LM A D L +I + D + D+R WLS I+Y+Q CM+ F++ N +
Sbjct: 127 KLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQD 186
Query: 174 KEQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLKF---DVKPASRRLLNSEVTVDDQG 230
++ S +++T+ L ++T +S++L +FN+ D+ +R+LL++E G
Sbjct: 187 MQKIFKTS----RELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAE-----DG 237
Query: 231 YPSWISSSDRKLLAKMQRKNWRANIMPNAVVAKDGSGQFKTIQAALASYPKGNKGRYVIY 290
PSW+ + R+L+A + + N VVA DGSGQ+KTI AL + PK N+ +VIY
Sbjct: 238 IPSWVGPNTRRLMAT------KGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIY 291
Query: 291 VKAGVYDEYITVPKDAVNILMYGDGPARTIVTGRKSFAAG-VKTMQTATFANTAMGFIGK 349
+K GVY+E + V K ++ GDGP +T +TG ++ G VKT TAT A F K
Sbjct: 292 IKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAK 351
Query: 350 AMTFENTAGPDGHQAVAFRNQGDMSALVGCHIVGYQDSLYVQSNRQYYRNCLVSGTVDFI 409
+ FENTAGP+GHQAVA R D++ C I GYQD+LYV S+RQ++R+C VSGTVDFI
Sbjct: 352 NIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFI 411
Query: 410 FGSSATLIQHSTIIVRKPGKGQFNTITADGSDTMNLNTGIVIQDCNIIPEAALFPERFTI 469
FG ++Q+ I+VRKP K Q ITA G +TG+V+Q+C+I E A P +
Sbjct: 412 FGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSIN 471
Query: 470 RSYLGRPWKYLAKTVVMESTIGDFIHPDGWTIWQGEQNHNTCYYAEYANTGPGANVARRV 529
++YLGRPWK ++T++M +TI D I P GW W G+ NT YYAEY N GPG+N A+RV
Sbjct: 472 KAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRV 531
Query: 530 KWKGYHGVISRAEANKFTAGIWLQAGPKSAAEWLNGLHVPHYLGFK 575
KW G +S +A +FT +L+ W+ VP+ F+
Sbjct: 532 KWPGIK-KLSPKQALRFTPARFLRGN-----LWIPPNRVPYMGNFQ 571
>dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
Length = 617
Score = 360 bits (923), Expect = 9e-98
Identities = 209/539 (38%), Positives = 300/539 (54%), Gaps = 33/539 (6%)
Query: 30 GEDPEVQTQQRNLRIMCQNSQDQKLCHETLSSVHGADAADPKAYIAASVKAATDNVIKAF 89
G +++ +++++MC + C ++ A + PK I +V V +AF
Sbjct: 85 GGKADLRAVSKSIKMMCAQTDFADSCATSIGKAANASVSSPKDIIRTAVDVIGGAVDQAF 144
Query: 90 NMSERLTTEYGKENGAKMALNDCKDLMQFALDSLDLSTKCVHDNNIQAVHDQIADMRNWL 149
+ ++ + + + K A+ DCK+L A D L+ + K + + Q +R WL
Sbjct: 145 DRADLIMSN---DPRVKAAVADCKELFDDAKDDLNCTLKGIDGKDGLK---QGFQLRVWL 198
Query: 150 SAVISYRQACMEGFDDANDGEKKIKEQFHVQSLDSVQKVTAVALDIVTGLSDILQQFNLK 209
SAVI+ + C++GF D + K+KE F+ + ++ T+ AL ++ S L
Sbjct: 199 SAVIANMETCIDGFPDG-EFRDKVKESFN-----NGREFTSNALALIEKASSFLSA---- 248
Query: 210 FDVKPASRRLLNSE-----------VTVDDQGYPSWISSSDRKLLAKMQRKNWRANIMPN 258
+K + RRLL E + + + G P W+ DR++L KN N+ PN
Sbjct: 249 --LKGSQRRLLAGEEDNGGGAADPHLALAEDGIPEWVPDGDRRVLKGGGFKN---NLTPN 303
Query: 259 AVVAKDGSGQFKTIQAALASYPKGNKGRYVIYVKAGVYDEYITVPKDAVNILMYGDGPAR 318
+VAKDGSG+FKTI ALA+ PK GRYVIYVK GVY EY+T+ K ++ MYGDG +
Sbjct: 304 VIVAKDGSGKFKTINEALAAMPKTYSGRYVIYVKEGVYAEYVTITKKMASVTMYGDGSRK 363
Query: 319 TIVTGRKSFAAGVKTMQTATFANTAMGFIGKAMTFENTAGPDGHQAVAFRNQGDMSALVG 378
+IVTG K+FA G+ T +TATFA GF+ M F+NTAG HQAVA Q D S +
Sbjct: 364 SIVTGSKNFADGLTTFKTATFAAQGDGFMAIGMGFQNTAGAAKHQAVALLVQSDKSVFLN 423
Query: 379 CHIVGYQDSLYVQSNRQYYRNCLVSGTVDFIFGSSATLIQHSTIIVRKPGKGQFNTITAD 438
C + G+QD+LY S Q+YRNC+++GT+DF+FG +A + Q+ + +R+P Q N TA
Sbjct: 424 CWMDGFQDTLYAHSKAQFYRNCVITGTIDFVFGDAAAVFQNCVLTLRRPMDNQQNIATAQ 483
Query: 439 GSDTMNLNTGIVIQDCNIIPEAALFPERF-TIRSYLGRPWKYLAKTVVMESTIGDFIHPD 497
G TG V+Q C E AL + IR+YLGRPW+ ++TV+MES I I
Sbjct: 484 GRADGREATGFVLQKCEFNAEPALTDAKLPPIRNYLGRPWREFSRTVIMESDIPAIIDKA 543
Query: 498 GWTIWQGEQNHNTCYYAEYANTGPGANVARRVKWKGYHGVISRAEANKFTAGIWLQAGP 556
G+ W GE T YYAEYAN GPGA+ A RV W GY VIS+A+A KFT +L A P
Sbjct: 544 GYMPWNGEFALKTLYYAEYANKGPGADTAGRVAWPGYKKVISKADATKFTVDNFLHAKP 602
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.318 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 958,623,056
Number of Sequences: 2540612
Number of extensions: 39272831
Number of successful extensions: 112626
Number of sequences better than 10.0: 378
Number of HSP's better than 10.0 without gapping: 310
Number of HSP's successfully gapped in prelim test: 68
Number of HSP's that attempted gapping in prelim test: 111427
Number of HSP's gapped (non-prelim): 448
length of query: 576
length of database: 863,360,394
effective HSP length: 134
effective length of query: 442
effective length of database: 522,918,386
effective search space: 231129926612
effective search space used: 231129926612
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 78 (34.7 bits)
Medicago: description of AC144563.2