BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144503.3 + phase: 0
(544 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL35216.1| 4-coumarate:CoA ligase [Amorpha fruticosa] 919 0.0
gb|AAC97600.1| 4-coumarate:CoA ligase isoenzyme 2 [Glycine max] 883 0.0
gb|AAV65114.1| 4-coumarate:CoA ligase [Betula platyphylla] 859 0.0
gb|AAK58908.1| 4-coumarate:CoA ligase 3 [Populus balsamifera sub... 832 0.0
sp|O24540|4CL_VANPL 4-coumarate--CoA ligase (4CL) (4-coumaroyl-C... 832 0.0
gb|AAL02144.1| 4-coumarate:CoA ligase [Populus tomentosa] 830 0.0
gb|AAB18637.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]... 829 0.0
emb|CAA31696.1| unnamed protein product [Petroselinum crispum] g... 828 0.0
gb|AAL02145.1| 4-coumarate:CoA ligase [Populus tomentosa] 828 0.0
pir||A39827 4-coumarate-CoA ligase (EC 6.2.1.12) 1 - potato gi|3... 828 0.0
pir||B39827 4-coumarate-CoA ligase (EC 6.2.1.12) 2a - potato gi|... 828 0.0
gb|AAB18638.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]... 827 0.0
emb|CAA31697.1| unnamed protein product [Petroselinum crispum] g... 826 0.0
gb|AAL56850.1| 4-coumarate:CoA ligase [Populus tomentosa] 826 0.0
gb|AAD40664.1| 4-coumarate:coenzyme A ligase [Solanum tuberosum] 824 0.0
gb|AAF91310.1| 4-coumarate:coA ligase 1 [Rubus idaeus] 823 0.0
pir||T02074 4-coumarate-CoA ligase (EC 6.2.1.12) - common tobacc... 820 0.0
gb|AAF91309.1| 4-coumarate:coA ligase 2 [Rubus idaeus] 820 0.0
gb|AAC24503.1| 4-coumarate:CoA ligase [Populus tremuloides] gi|7... 816 0.0
gb|AAP68991.1| 4-coumarate:coenzyme A ligase 2 [Salvia miltiorrh... 811 0.0
>gb|AAL35216.1| 4-coumarate:CoA ligase [Amorpha fruticosa]
Length = 540
Score = 919 bits (2376), Expect = 0.0
Identities = 459/545 (84%), Positives = 510/545 (93%), Gaps = 6/545 (1%)
Query: 1 MTIQTME-QELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQ 59
M +T E +E IF+SKLP+I I KHLPLHSYCFENLS++GSRPCLI+APT ++YTYYDV+
Sbjct: 1 MAFETEEPKEFIFRSKLPEIPISKHLPLHSYCFENLSEFGSRPCLISAPTGDVYTYYDVE 60
Query: 60 LTAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQ 119
LTA++VASGLNKLG+QQGDVIM+LLPN PEFVF+FLGAS+RGA++TAANPFFTSAEIAKQ
Sbjct: 61 LTARRVASGLNKLGVQQGDVIMLLLPNSPEFVFSFLGASYRGAMITAANPFFTSAEIAKQ 120
Query: 120 AKASNTKLLVTQACYYDKVKDLENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNI 179
AKASNTKLL+TQA YYDKVKDL+ VKLVFVDS P D HMH+SEL +AD+++M EVK N
Sbjct: 121 AKASNTKLLITQASYYDKVKDLD-VKLVFVDSPP--DGHMHYSELREADESDMPEVKTN- 176
Query: 180 KPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHI 239
PDDVVALPYSSGTTGLPKGVML+HKGL TSIAQQVDGENPNLY+H+EDVILCVLP+FHI
Sbjct: 177 -PDDVVALPYSSGTTGLPKGVMLSHKGLATSIAQQVDGENPNLYFHNEDVILCVLPLFHI 235
Query: 240 YSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYD 299
YSLNSVLLCGLRAKA+ILLMPKF+INA GL+ K++VTIAP+VPPIVLAIAKSP+L+KYD
Sbjct: 236 YSLNSVLLCGLRAKAAILLMPKFEINALLGLIQKHRVTIAPIVPPIVLAIAKSPDLEKYD 295
Query: 300 LSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSG 359
LSSIRVLKSGGA LGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCL+FAKEPIDVK G
Sbjct: 296 LSSIRVLKSGGASLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLAFAKEPIDVKPG 355
Query: 360 ACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHT 419
ACGTVVRNAEMKIVDP+ +SLPRNQ GEICIRGDQIMKGYLN+ EAT+ TIDKEGWLHT
Sbjct: 356 ACGTVVRNAEMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDQEATQRTIDKEGWLHT 415
Query: 420 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEV 479
GDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++LSHP+I+D AVVPM DEAAGEV
Sbjct: 416 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPKITDAAVVPMKDEAAGEV 475
Query: 480 PVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLA 539
PVAFVVRSNG DTTED+IK+F+SKQVVFYKRI+RVFFIDAIPKSPSGKILRKDLRAKLA
Sbjct: 476 PVAFVVRSNGHTDTTEDEIKQFISKQVVFYKRISRVFFIDAIPKSPSGKILRKDLRAKLA 535
Query: 540 AGVPN 544
AGVPN
Sbjct: 536 AGVPN 540
>gb|AAC97600.1| 4-coumarate:CoA ligase isoenzyme 2 [Glycine max]
Length = 547
Score = 883 bits (2282), Expect = 0.0
Identities = 438/539 (81%), Positives = 485/539 (89%), Gaps = 3/539 (0%)
Query: 8 QELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVAS 67
+ELIF+SKLPDIYIPKH+PLHSYCFENL + GSRPCLINAPT ++Y+Y++V TA+KVA
Sbjct: 8 RELIFRSKLPDIYIPKHMPLHSYCFENLRECGSRPCLINAPTGDVYSYHEVDSTARKVAR 67
Query: 68 GLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTKL 127
GL K G++QG VIM+LLPNCPEFVF+FLGAS RGA+ TAANPFFT AEIAKQA ASN KL
Sbjct: 68 GLKKEGVEQGQVIMILLPNCPEFVFSFLGASHRGAMATAANPFFTPAEIAKQAHASNAKL 127
Query: 128 LVTQACYYDKVKDLENVKLVFVDSSP---EEDNHMHFSELIQADQNEMEEVKVNIKPDDV 184
L+TQA YYDKVKDL ++KLVFVDS P EE H+HFS L + + + +V V+IKPDDV
Sbjct: 128 LITQASYYDKVKDLRDIKLVFVDSCPPHTEEKQHLHFSHLCEDNGDADVDVDVDIKPDDV 187
Query: 185 VALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLNS 244
VALPYSSGTTGLPKGVML+HKGLVTSIAQQVDG+NPNLYYH D ILCVLP+FHIYSLNS
Sbjct: 188 VALPYSSGTTGLPKGVMLSHKGLVTSIAQQVDGDNPNLYYHCHDTILCVLPLFHIYSLNS 247
Query: 245 VLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSIR 304
VLLCGLRAKA+ILLMPKFDIN+ L+ K+KVTIAPVVPPIVLAI+KSP+L KYDLSSIR
Sbjct: 248 VLLCGLRAKATILLMPKFDINSLLALIHKHKVTIAPVVPPIVLAISKSPDLHKYDLSSIR 307
Query: 305 VLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGTV 364
VLKSGGAPLGKELEDT+RAKFP AKLGQGYGMTEAGPVLTM L+FAKEPIDVK GACGTV
Sbjct: 308 VLKSGGAPLGKELEDTLRAKFPNAKLGQGYGMTEAGPVLTMSLAFAKEPIDVKPGACGTV 367
Query: 365 VRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIGF 424
VRNAEMKIVDP+ SLPRNQ GEICIRGDQIMKGYLN+ EAT TIDK+GWLHTGDIG+
Sbjct: 368 VRNAEMKIVDPETGHSLPRNQSGEICIRGDQIMKGYLNDGEATERTIDKDGWLHTGDIGY 427
Query: 425 IDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAFV 484
IDDDDELFIVDRLKELIKYKGFQVAPAELEA++L+HP+ISD AVVPM DEAAGEVPVAFV
Sbjct: 428 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVPMKDEAAGEVPVAFV 487
Query: 485 VRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAGVP 543
V SNG DTTED+IK+F+SKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAK+AA VP
Sbjct: 488 VISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKIAASVP 546
>gb|AAV65114.1| 4-coumarate:CoA ligase [Betula platyphylla]
Length = 542
Score = 859 bits (2220), Expect = 0.0
Identities = 421/547 (76%), Positives = 486/547 (87%), Gaps = 8/547 (1%)
Query: 1 MTIQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQL 60
M IQ EQE IF+SKLPDIYIPKHLP+HSYCFEN+SK GSRPCLIN T ++YTYYDV+L
Sbjct: 1 MAIQAKEQEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVEL 60
Query: 61 TAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQA 120
TA+KVASGL+KLGIQ+GDV+M+LLPN PEF F FLGAS+ GA+ TAANPF T+ E++KQA
Sbjct: 61 TARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQA 120
Query: 121 KASNTKLLVTQACYYDKVKDLEN---VKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKV 177
K++N K++VTQACYYD+VKD N VK++ +DS PE+ +HFSEL +AD+N++ EV
Sbjct: 121 KSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDC--LHFSELTKADENDVAEV-- 176
Query: 178 NIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMF 237
+I PDDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLYYHSEDVILCVLP+F
Sbjct: 177 DISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLF 236
Query: 238 HIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDK 297
HIYSLNSV LCGLRA ASIL++PKF+I + L+ K+KVT+ P+VPPIVLAI K P+LDK
Sbjct: 237 HIYSLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDK 296
Query: 298 YDLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVK 357
YDLSS+++LKSGGAPLGKE+E+TV+AKFP A GQGYGMTEAGPVL MCL+FAKEP++VK
Sbjct: 297 YDLSSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVK 356
Query: 358 SGACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWL 417
SGACGTVVRNAEMKIVDP+ +SLPRNQPGEICIRGDQIMKGY+N+PEAT TIDKEGWL
Sbjct: 357 SGACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWL 416
Query: 418 HTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAG 477
HTGDIG IDD+DELFIVDRLKELIKYKGFQVAPAELEA++L+HP ISD AVVPM D+ AG
Sbjct: 417 HTGDIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAG 476
Query: 478 EVPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAK 537
EVPVAFV RSNGS TED+IK+FVSKQVVFYKRI+RVFFID +PKSPSGKILRK+LRAK
Sbjct: 477 EVPVAFVARSNGS-QVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAK 535
Query: 538 LAAGVPN 544
LAAG N
Sbjct: 536 LAAGFSN 542
>gb|AAK58908.1| 4-coumarate:CoA ligase 3 [Populus balsamifera subsp. trichocarpa x
Populus deltoides]
Length = 540
Score = 832 bits (2150), Expect = 0.0
Identities = 411/539 (76%), Positives = 473/539 (87%), Gaps = 8/539 (1%)
Query: 3 IQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTA 62
I ++E IF+SKLPDIYIPK+LPLHSY ENLSKY S+PCLIN ++YTY DV+LTA
Sbjct: 4 IMNSQEEFIFRSKLPDIYIPKNLPLHSYVLENLSKYSSKPCLINGANGDVYTYADVELTA 63
Query: 63 QKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKA 122
++VASGLNK+GIQQGDVIM+ LP+ PEFV AFLGAS RGAI+TAANPF T AE+AK AK
Sbjct: 64 RRVASGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAIVTAANPFSTPAELAKHAKP 123
Query: 123 SNTKLLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNI 179
TKLL+TQACYYDKVKD +VK++ VDS+P D +HFSEL QAD+NE+ +V +
Sbjct: 124 PRTKLLITQACYYDKVKDFARESDVKVMCVDSAP--DGCLHFSELTQADENEVPQV--DF 179
Query: 180 KPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHI 239
PDDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+HSEDVILCVLPMFHI
Sbjct: 180 SPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVILCVLPMFHI 239
Query: 240 YSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYD 299
Y+LNS++LCGLR ASIL+MPKFDI GL+ KYKV+IAPVVPP++LAIAKSP+ DK+D
Sbjct: 240 YALNSIMLCGLRVGASILIMPKFDIGTLLGLIEKYKVSIAPVVPPVMLAIAKSPDFDKHD 299
Query: 300 LSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSG 359
LSS+R++KSGGAPLGKELEDTVRAKFP+A+LGQGYGMTEAGPVL MCL+FAKEP D+K G
Sbjct: 300 LSSLRMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPG 359
Query: 360 ACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHT 419
ACGTVVRNAEMKIVDP+ +SL RNQPGEICIRGDQIMKGYLN+PEAT TIDKEGWLHT
Sbjct: 360 ACGTVVRNAEMKIVDPETGASLRRNQPGEICIRGDQIMKGYLNDPEATSRTIDKEGWLHT 419
Query: 420 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEV 479
GDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++L+HPQISD AVV M DE AGEV
Sbjct: 420 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLAHPQISDAAVVGMKDEDAGEV 479
Query: 480 PVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKL 538
PVAFVV+S S TED+IK+++SKQV+FYKRI RVFFI+AIPK+PSGKILRK+LR L
Sbjct: 480 PVAFVVKSEKS-QATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLRETL 537
>sp|O24540|4CL_VANPL 4-coumarate--CoA ligase (4CL) (4-coumaroyl-CoA synthase)
Length = 553
Score = 832 bits (2149), Expect = 0.0
Identities = 414/546 (75%), Positives = 472/546 (85%), Gaps = 9/546 (1%)
Query: 1 MTIQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQL 60
+ I+ ++++IF+SKLPDIYIPK+LPLHSYCFEN+SK+ SRPCLIN T EI+TY DV+L
Sbjct: 5 VAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVEL 64
Query: 61 TAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQA 120
+++V SGL+KLGI+QGD IM+LLPN PEFVFAFLGASF G+I T ANPFFTS E+ KQA
Sbjct: 65 ISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQA 124
Query: 121 KASNTKLLVTQACYYDKVKDL---ENVKLVFVDSSPEED---NHMHFSELIQADQNEMEE 174
KASN KL++TQ CY DKVKD VK++ +D++ D N +HFSEL AD+NEM
Sbjct: 125 KASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMP- 183
Query: 175 VKVNIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVL 234
KV I PD VVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLY HS+DV+LCVL
Sbjct: 184 -KVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 242
Query: 235 PMFHIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPE 294
P+FHIYSLNSVLLCGLRA + IL+M KF+I F L+ KYKVTI P VPPIVLAIAKS
Sbjct: 243 PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 302
Query: 295 LDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPI 354
+D YDLSS+R + SG APLGKELED VRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP
Sbjct: 303 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 362
Query: 355 DVKSGACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKE 414
D+KSGACGTVVRNAEMKIVDP+ SSLPRN PGEICIRGDQIMKGYLN+PEAT TIDKE
Sbjct: 363 DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 422
Query: 415 GWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDE 474
GWLHTGDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++L+HP ISD AVVPM DE
Sbjct: 423 GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 482
Query: 475 AAGEVPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDL 534
AAGEVPVAFVV+SNG + TED+IK+F+SKQV+FYKRINRVFF++AIPK+PSGKILRKDL
Sbjct: 483 AAGEVPVAFVVKSNGH-NITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDL 541
Query: 535 RAKLAA 540
RA+LAA
Sbjct: 542 RARLAA 547
>gb|AAL02144.1| 4-coumarate:CoA ligase [Populus tomentosa]
Length = 536
Score = 830 bits (2144), Expect = 0.0
Identities = 406/536 (75%), Positives = 477/536 (88%), Gaps = 8/536 (1%)
Query: 7 EQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVA 66
++E IF+SKLPDIYIPK+LPLHSY ENLS + S+PCLIN ++YTY DV+LTA++VA
Sbjct: 4 QEEFIFRSKLPDIYIPKNLPLHSYVLENLSNHSSKPCLINGANGDVYTYADVELTARRVA 63
Query: 67 SGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTK 126
SGLNK+GIQQGDVIM+ LP+ PEFV AFLGAS RGAI+TAANPF T AE+AK AKAS K
Sbjct: 64 SGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAIITAANPFSTPAELAKHAKASRAK 123
Query: 127 LLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDD 183
LL+TQACYY+KVKD +VK++ VDS+P D +HFSEL QAD+NE +V +I PDD
Sbjct: 124 LLITQACYYEKVKDFARESDVKVMCVDSAP--DGCLHFSELTQADENEAPQV--DISPDD 179
Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLN 243
VVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+HSEDVILCVLPMFHIY+LN
Sbjct: 180 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVILCVLPMFHIYALN 239
Query: 244 SVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSI 303
S++LCGLR ASIL+MPKF+I + GL+ KYKV+IAPVVPP++++IAKSP+LDK+DLSS+
Sbjct: 240 SIMLCGLRVGASILIMPKFEIGSLLGLIEKYKVSIAPVVPPVMMSIAKSPDLDKHDLSSL 299
Query: 304 RVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGT 363
R++KSGGAPLGKELEDTVRAKFP+A+LGQGYGMTEAGPVL MCL+FAKEP D+K GACGT
Sbjct: 300 RMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGACGT 359
Query: 364 VVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIG 423
VVRNAEMKIVDP+ +SLPRNQPGEICIRGDQIMKGYLN+PEAT TIDKEGWLHTGDIG
Sbjct: 360 VVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKEGWLHTGDIG 419
Query: 424 FIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAF 483
+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++++HP+ISD AVV + DE AGEVPVAF
Sbjct: 420 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGLKDEDAGEVPVAF 479
Query: 484 VVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLA 539
VV+S S TED+IK+++SKQV+FYKRI RVFFI+AIPK+PSGKILRK+L+ KLA
Sbjct: 480 VVKSEKS-QATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLKEKLA 534
>gb|AAB18637.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]
gi|12229631|sp|O24145|4CL1_TOBAC 4-coumarate--CoA ligase
1 (4CL 1) (4-coumaroyl-CoA synthase 1)
Length = 547
Score = 829 bits (2142), Expect = 0.0
Identities = 408/539 (75%), Positives = 467/539 (85%), Gaps = 8/539 (1%)
Query: 9 ELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVASG 68
+LIF+SKLPDIYIPKHLPLHSYCFEN+S++ SRPCLIN +IYTY +V+LT +KVA G
Sbjct: 14 DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVG 73
Query: 69 LNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTKLL 128
LNKLGIQQ D IM+LLPN PEFVFAF+GAS+ GAI T ANP FT AE+ KQAKAS+ K++
Sbjct: 74 LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKII 133
Query: 129 VTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDDVV 185
+TQ+C+ KVKD +VK++ +DS+PE +HFSEL Q+D++E+ EVK I+PDDVV
Sbjct: 134 ITQSCFVGKVKDYASENDVKVICIDSAPE--GCLHFSELTQSDEHEIPEVK--IQPDDVV 189
Query: 186 ALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLNSV 245
ALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGEN NLY HSEDV++CVLP+FHIYSLNS+
Sbjct: 190 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSI 249
Query: 246 LLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSIRV 305
LLCGLR A+IL+M KFDI F L+ KYKV+I P VPPIVLAIAKSP +D YDLSS+R
Sbjct: 250 LLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRT 309
Query: 306 LKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGTVV 365
+ SG APLGKELED VR KFP AKLGQGYGMTEAGPVL MCL+FAKEP D+KSGACGTVV
Sbjct: 310 VMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 369
Query: 366 RNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIGFI 425
RNAEMKIVDP SLPRNQPGEICIRGDQIMKGYLN+PEAT TIDKEGWLHTGDIGFI
Sbjct: 370 RNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFI 429
Query: 426 DDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAFVV 485
D+DDELFIVDRLKELIKYKGFQVAPAE+EA++L+HP ISD AVVPM DE AGEVPVAFVV
Sbjct: 430 DEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVV 489
Query: 486 RSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 544
RSNGS TED++K F+SKQV+FYKR+ RVFF++ +PKSPSGKILRKDLRA+LAAGVPN
Sbjct: 490 RSNGSA-ITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547
>emb|CAA31696.1| unnamed protein product [Petroselinum crispum] gi|82059|pir||S01667
4-coumarate-CoA ligase (EC 6.2.1.12) (clone pc4CL-1) -
parsley gi|112800|sp|P14912|4CL1_PETCR 4-coumarate--CoA
ligase 1 (4CL 1) (4-coumaroyl-CoA synthase 1)
Length = 544
Score = 828 bits (2139), Expect = 0.0
Identities = 406/538 (75%), Positives = 474/538 (87%), Gaps = 8/538 (1%)
Query: 7 EQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVA 66
+++LIF+SKLPDIYIPKHLPLH+YCFEN+SK G + CLIN T E +TY V+L ++KVA
Sbjct: 8 KEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVA 67
Query: 67 SGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTK 126
SGLNKLGIQQGD IM+LLPN PE+ FAFLGAS+RGAI T ANPFFTSAE+ KQ KAS K
Sbjct: 68 SGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAK 127
Query: 127 LLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDD 183
L++TQACY DKVKD +N++++ +D +P++ +HFS+L++AD++EM EV +N DD
Sbjct: 128 LIITQACYVDKVKDYAAEKNIQIICIDDAPQDC--LHFSKLMEADESEMPEVVIN--SDD 183
Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLN 243
VVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDG+NPNLY HSEDV++C+LP+FHIYSLN
Sbjct: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLN 243
Query: 244 SVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSI 303
+VL CGLRA +IL+M KFDI F L+ KYKVTI P VPPIVLAIAKSP +DKYDLSS+
Sbjct: 244 AVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSV 303
Query: 304 RVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGT 363
R + SG APLGKELED VRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP ++KSGACGT
Sbjct: 304 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 363
Query: 364 VVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIG 423
VVRNAEMKIVDP+ ++SLPRNQ GEICIRGDQIMKGYLN+PE+TR TID+EGWLHTGDIG
Sbjct: 364 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 423
Query: 424 FIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAF 483
FIDDDDELFIVDRLKE+IKYKGFQVAPAELEA++L+HP ISD AVVPM+DE AGEVPVAF
Sbjct: 424 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 483
Query: 484 VVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAG 541
VVR+NG TTE++IK+FVSKQVVFYKRI RVFF+DAIPKSPSGKILRKDLRA++A+G
Sbjct: 484 VVRTNG-FTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540
>gb|AAL02145.1| 4-coumarate:CoA ligase [Populus tomentosa]
Length = 536
Score = 828 bits (2139), Expect = 0.0
Identities = 405/536 (75%), Positives = 476/536 (88%), Gaps = 8/536 (1%)
Query: 7 EQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVA 66
++E IF+SKLPDIYIPK+LPLHSY ENLS + S+PCLIN ++YTY DV+LTA++VA
Sbjct: 4 QEEFIFRSKLPDIYIPKNLPLHSYVLENLSNHSSKPCLINGANGDVYTYADVELTARRVA 63
Query: 67 SGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTK 126
SGLNK+GIQQGDVIM+ LP+ PEFV AFLGAS RGAI+TAANPF T AE+AK AKAS K
Sbjct: 64 SGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAIITAANPFSTPAELAKHAKASRAK 123
Query: 127 LLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDD 183
LL+TQACYY+KVKD +VK++ VDS+P D +HFSEL QAD+NE +V +I PDD
Sbjct: 124 LLITQACYYEKVKDFARESDVKVMCVDSAP--DGCLHFSELTQADENEAPQV--DISPDD 179
Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLN 243
VVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+HSEDVILCVLPMFHIY+LN
Sbjct: 180 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVILCVLPMFHIYALN 239
Query: 244 SVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSI 303
S++LCGLR A IL+MPKF+I + GL+ KYKV+IAPVVPP++++IAKSP+LDK+DLSS+
Sbjct: 240 SIMLCGLRVGAPILIMPKFEIGSLLGLIEKYKVSIAPVVPPVMMSIAKSPDLDKHDLSSL 299
Query: 304 RVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGT 363
R++KSGGAPLGKELEDTVRAKFP+A+LGQGYGMTEAGPVL MCL+FAKEP D+K GACGT
Sbjct: 300 RMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGACGT 359
Query: 364 VVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIG 423
VVRNAEMKIVDP+ +SLPRNQPGEICIRGDQIMKGYLN+PEAT TIDKEGWLHTGDIG
Sbjct: 360 VVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKEGWLHTGDIG 419
Query: 424 FIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAF 483
+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++++HP+ISD AVV + DE AGEVPVAF
Sbjct: 420 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGLKDEDAGEVPVAF 479
Query: 484 VVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLA 539
VV+S S TED+IK+++SKQV+FYKRI RVFFI+AIPK+PSGKILRK+L+ KLA
Sbjct: 480 VVKSEKS-QATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLKEKLA 534
>pir||A39827 4-coumarate-CoA ligase (EC 6.2.1.12) 1 - potato
gi|398963|sp|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1
(4CL 1) (4-coumaroyl-CoA synthase 1)
gi|169574|gb|AAA33842.1| 4-coumarate--CoA ligase
Length = 545
Score = 828 bits (2138), Expect = 0.0
Identities = 411/539 (76%), Positives = 470/539 (86%), Gaps = 8/539 (1%)
Query: 9 ELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVASG 68
+LIF+SKLPDIYIPKHLPLHSYCFENLS++ SRPCLI+ IYTY +V+LT++KVA G
Sbjct: 12 DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVG 71
Query: 69 LNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTKLL 128
LNKLGIQQ D IM+LLPNCPEFVFAF+GAS+ GAI T ANP FT AE+ KQAKAS+ K++
Sbjct: 72 LNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIV 131
Query: 129 VTQACYYDKVKD--LEN-VKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDDVV 185
+TQAC+ KVKD +EN +K++ VDS PE +HFSELIQ+D++E+ +VK I+PDDVV
Sbjct: 132 ITQACFAGKVKDYAIENDLKVICVDSVPE--GCVHFSELIQSDEHEIPDVK--IQPDDVV 187
Query: 186 ALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLNSV 245
ALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGEN NLY HS+DV++CVLP+FHIYSLNSV
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSV 247
Query: 246 LLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSIRV 305
LLC LR A+IL+M KFDI F L+ K+KVTI P VPPIVLAIAKSP +D YDLSS+R
Sbjct: 248 LLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVRT 307
Query: 306 LKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGTVV 365
+ SG APLGKELED VRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP D+KSGACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 367
Query: 366 RNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIGFI 425
RNAEMKIVDP SLPRNQPGEICIRGDQIMKGYLN+PEAT TI+KEGWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFI 427
Query: 426 DDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAFVV 485
DDDDELFIVDRLKELIKYKGFQVAPAELEA++++HP ISD AVVPM+DE AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVV 487
Query: 486 RSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 544
RSNGS TED++K F+SKQV+FYKRI RVFF++ +PKSPSGKILRKDLRA+LAAG+ N
Sbjct: 488 RSNGS-TITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGISN 545
>pir||B39827 4-coumarate-CoA ligase (EC 6.2.1.12) 2a - potato
gi|398965|sp|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2
(4CL 2) (4-coumaroyl-CoA synthase 2)
Length = 545
Score = 828 bits (2138), Expect = 0.0
Identities = 413/548 (75%), Positives = 475/548 (86%), Gaps = 9/548 (1%)
Query: 1 MTIQTMEQ-ELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQ 59
M I+T + +LIF+SKLPDIYIPKHLPLHSYCFENLS++ SRPCLI+ IYTY +V+
Sbjct: 3 MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62
Query: 60 LTAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQ 119
LT++KVA GLNKLGIQQ D IM+LLPNCPEFVFAF+GAS+ GAI T ANP FT AE+ KQ
Sbjct: 63 LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122
Query: 120 AKASNTKLLVTQACYYDKVKD--LEN-VKLVFVDSSPEEDNHMHFSELIQADQNEMEEVK 176
AKAS+ K+++TQAC+ KVKD +EN +K++ VDS+PE +HFSELIQ+D++E+ +VK
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPE--GCVHFSELIQSDEHEIPDVK 180
Query: 177 VNIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPM 236
I+PDDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGEN NLY HS+DV++CVLP+
Sbjct: 181 --IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPL 238
Query: 237 FHIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELD 296
FHIYSLNSVLLC LR A+IL+M KFDI F L+ K+KVTI P VPPIVLAIAKSP +
Sbjct: 239 FHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVH 298
Query: 297 KYDLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDV 356
YDLSS+R + SG APLGKELED VRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP D+
Sbjct: 299 NYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDI 358
Query: 357 KSGACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGW 416
KSGACGTVVRNAEMKIVDP SLPRNQPGEICIRGDQIMKGYLN+PEAT TI+KEGW
Sbjct: 359 KSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGW 418
Query: 417 LHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAA 476
LHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEA++++HP ISD AVVPM+DE A
Sbjct: 419 LHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQA 478
Query: 477 GEVPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRA 536
GEVPVAFVVRSNGS TED++K F+SKQV+FYKRI RVFF++ +PKSPSGKILRKDLRA
Sbjct: 479 GEVPVAFVVRSNGS-TITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 537
Query: 537 KLAAGVPN 544
+LAAG+ N
Sbjct: 538 RLAAGISN 545
>gb|AAB18638.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]
gi|7437848|pir||T03789 4-coumarate-CoA ligase (EC
6.2.1.12) 4CL2 - common tobacco
gi|12229632|sp|O24146|4CL2_TOBAC 4-coumarate--CoA ligase
2 (4CL 2) (4-coumaroyl-CoA synthase 2)
Length = 542
Score = 827 bits (2136), Expect = 0.0
Identities = 411/547 (75%), Positives = 474/547 (86%), Gaps = 8/547 (1%)
Query: 1 MTIQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQL 60
M T + ++IF+SKLPDIYIP HLPLHSYCFEN+S++ SRPCLIN +IYTY DV+L
Sbjct: 1 MEKDTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVEL 60
Query: 61 TAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQA 120
++KVA+GL+K GIQ D IM+LLPN PEFVFAF+GAS+ GAI T ANP FT AE+ KQA
Sbjct: 61 NSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQA 120
Query: 121 KASNTKLLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKV 177
KAS+ K++VTQAC+ +KVKD +VK++ +DS+PE +HFS L QA+++++ EV+
Sbjct: 121 KASSAKIIVTQACHVNKVKDYAFENDVKIICIDSAPE--GCLHFSVLTQANEHDIPEVE- 177
Query: 178 NIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMF 237
I+PDDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLY HSEDV+LCVLP+F
Sbjct: 178 -IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLF 236
Query: 238 HIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDK 297
HIYSLNSVLLCGLR A+IL+M KFDI +F L+ +YKVTI P VPPIVLAIAKSP +D
Sbjct: 237 HIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDD 296
Query: 298 YDLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVK 357
YDLSS+R + SG APLGKELEDTVRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP ++K
Sbjct: 297 YDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIK 356
Query: 358 SGACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWL 417
SGACGTVVRNAEMKIVDP+ +SLPRNQ GEICIRGDQIMKGYLN+PEAT TIDKEGWL
Sbjct: 357 SGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWL 416
Query: 418 HTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAG 477
+TGDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++L+HP ISD AVVPM DE AG
Sbjct: 417 YTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAG 476
Query: 478 EVPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAK 537
EVPVAFVVRSNGS TED++K F+SKQV+FYKRI RVFF+DAIPKSPSGKILRKDLRAK
Sbjct: 477 EVPVAFVVRSNGS-TITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAK 535
Query: 538 LAAGVPN 544
LAAG+PN
Sbjct: 536 LAAGLPN 542
>emb|CAA31697.1| unnamed protein product [Petroselinum crispum]
gi|282937|pir||S15695 4-coumarate-CoA ligase (EC
6.2.1.12) (clone Pc4CL-2) - parsley
gi|112801|sp|P14913|4CL2_PETCR 4-coumarate--CoA ligase 1
(4CL 1) (4-coumaroyl-CoA synthase 1)
Length = 544
Score = 826 bits (2134), Expect = 0.0
Identities = 406/538 (75%), Positives = 474/538 (87%), Gaps = 8/538 (1%)
Query: 7 EQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVA 66
+++LIF+SKLPDIYIPKHLPLH+YCFEN+SK G + CLIN T E +TY V+L ++KVA
Sbjct: 8 KEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVA 67
Query: 67 SGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTK 126
SGLNKLGIQQGD IM+LLPN PE+ FAFLGAS+RGAI T ANPFFTSAE+ KQ KAS K
Sbjct: 68 SGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAK 127
Query: 127 LLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDD 183
L++TQACY DKVKD +N++++ +D +P++ +HFS+L++AD++EM EV ++ DD
Sbjct: 128 LIITQACYVDKVKDYAAEKNIQIICIDDAPQDC--LHFSKLMEADESEMPEVVID--SDD 183
Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLN 243
VVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDG+NPNLY HSEDV++C+LP+FHIYSLN
Sbjct: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLN 243
Query: 244 SVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSI 303
+VL CGLRA +IL+M KFDI F L+ KYKVTI P VPPIVLAIAKSP +DKYDLSS+
Sbjct: 244 AVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSV 303
Query: 304 RVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGT 363
R + SG APLGKELED VRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP ++KSGACGT
Sbjct: 304 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 363
Query: 364 VVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIG 423
VVRNAEMKIVDP+ ++SLPRNQ GEICIRGDQIMKGYLN+PE+TR TID+EGWLHTGDIG
Sbjct: 364 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 423
Query: 424 FIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAF 483
FIDDDDELFIVDRLKE+IKYKGFQVAPAELEA++L+HP ISD AVVPM+DE AGEVPVAF
Sbjct: 424 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 483
Query: 484 VVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAG 541
VVR+NG TTE++IK+FVSKQVVFYKRI RVFF+DAIPKSPSGKILRKDLRAK+A+G
Sbjct: 484 VVRTNG-FTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASG 540
>gb|AAL56850.1| 4-coumarate:CoA ligase [Populus tomentosa]
Length = 536
Score = 826 bits (2134), Expect = 0.0
Identities = 406/535 (75%), Positives = 473/535 (87%), Gaps = 8/535 (1%)
Query: 7 EQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVA 66
++E IF+SKLPDIYIPK+LPLHSY ENLSK+ S+PCLIN ++YT DV+LTA++VA
Sbjct: 4 QEEFIFRSKLPDIYIPKNLPLHSYVLENLSKHSSKPCLINGANGDVYTCADVELTARRVA 63
Query: 67 SGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTK 126
SGLNK+GIQQGDVIM+ LP+ PEFV AFLGAS RGAI TAANPF T AE+AK AKAS K
Sbjct: 64 SGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAITTAANPFSTPAELAKHAKASRAK 123
Query: 127 LLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDD 183
LL+TQACYY+KVKD +VK++ VDS+P D +HFSEL QAD+NE +V +I PDD
Sbjct: 124 LLITQACYYEKVKDFARESDVKVMCVDSAP--DGCLHFSELTQADENEAPQV--DISPDD 179
Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLN 243
VVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+HSEDVILCVLPMFHIY+LN
Sbjct: 180 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVILCVLPMFHIYALN 239
Query: 244 SVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSI 303
S++LCGLR ASIL+MPKF+I GL+ KYKV+IAPVVPP++LAIA+SP+LDK+DLSS+
Sbjct: 240 SIMLCGLRVGASILIMPKFEIGTLLGLIEKYKVSIAPVVPPVMLAIARSPDLDKHDLSSL 299
Query: 304 RVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGT 363
R++KSGGAPLGKELEDTVRAKFP+A+LGQGYGMTEAGPVL MCL+FAKEP D+K GACGT
Sbjct: 300 RMIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGACGT 359
Query: 364 VVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIG 423
VVRNAEMKIVDP+ +SLPRNQPGEICIRGDQIMKGYLN+PEAT TIDKEGWLHTGDIG
Sbjct: 360 VVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKEGWLHTGDIG 419
Query: 424 FIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAF 483
+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++++HP+ISD AVV M DE AGEVPVAF
Sbjct: 420 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGMKDEDAGEVPVAF 479
Query: 484 VVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKL 538
V+S S TED+IK+++SKQV+FYKRI RVFFI+AIPK+PSGKILRK+L+ KL
Sbjct: 480 AVKSEKS-QATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLKEKL 533
>gb|AAD40664.1| 4-coumarate:coenzyme A ligase [Solanum tuberosum]
Length = 545
Score = 824 bits (2128), Expect = 0.0
Identities = 409/539 (75%), Positives = 470/539 (86%), Gaps = 8/539 (1%)
Query: 9 ELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVASG 68
+LIF+SKLPDIYIPKHLPLHSYCFENLS++ SRPCLI+ IYTY +V+LT++KVA G
Sbjct: 12 DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVG 71
Query: 69 LNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTKLL 128
LNKLGIQQ D IM+LLPNCPEFVFAF+GAS+ GAI T ANP FT AE+ KQAKAS+ K++
Sbjct: 72 LNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 131
Query: 129 VTQACYYDKVKD--LEN-VKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDDVV 185
+TQAC+ KVKD +EN +K++ VDS+PE +HFSELIQ+D++E+ +VK I+PDDVV
Sbjct: 132 ITQACFAGKVKDYAIENDLKVICVDSAPE--GCVHFSELIQSDEHEIPDVK--IQPDDVV 187
Query: 186 ALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLNSV 245
ALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGEN NLY HS+DV++CVLP+FHIYSLNSV
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSV 247
Query: 246 LLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSIRV 305
LLC LR A+IL+M KFDI F L+ K+KVTI P VPPIVLAIAKSP + YDLSS+R
Sbjct: 248 LLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDLSSVRT 307
Query: 306 LKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGTVV 365
+ SG APLGKELED VRAKFP AKLGQGYGMTEAG VLTMCL+FAKEP D+KSGACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGTVLTMCLAFAKEPFDIKSGACGTVV 367
Query: 366 RNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIGFI 425
RNAEMKIVDP SLPRNQPGEICIRGDQIMKGYLN+PEAT TI++EGWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEEEGWLHTGDIGFI 427
Query: 426 DDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAFVV 485
DDDDELFIVDRLKELIKYKGFQVAPAELEA++++HP ISD AVVPM+DE AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVV 487
Query: 486 RSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 544
RSNGS TED++K F+SKQV+FYKRI RVFF++ +PKSPSGKILRKDLRA+LAAG+ N
Sbjct: 488 RSNGS-TITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGISN 545
>gb|AAF91310.1| 4-coumarate:coA ligase 1 [Rubus idaeus]
Length = 543
Score = 823 bits (2126), Expect = 0.0
Identities = 409/546 (74%), Positives = 474/546 (85%), Gaps = 5/546 (0%)
Query: 1 MTIQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQL 60
M +QT + ++++SKLPDI+IP HLPLHSY F+N S S+PC+IN T +I+TY +L
Sbjct: 1 MAVQTPQHNIVYRSKLPDIHIPNHLPLHSYIFQNKSHLTSKPCIINGTTGDIHTYAKFKL 60
Query: 61 TAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQA 120
TA+KVASGLNKLGI++GDV M+LLPN EFVFAFLGASF GA+MTAANPFFT AEIAKQA
Sbjct: 61 TARKVASGLNKLGIEKGDVFMLLLPNTSEFVFAFLGASFCGAMMTAANPFFTPAEIAKQA 120
Query: 121 KASNTKLLVTQACYYDKVKDL--ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVN 178
KAS KL++T ACYYDKVKDL + VKL+ +DS P + + +HFSEL Q+D+N++ +V +
Sbjct: 121 KASKAKLIITFACYYDKVKDLSCDEVKLMCIDSPPPDSSCLHFSELTQSDENDVPDV--D 178
Query: 179 IKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFH 238
I PDDVVALPYSSGTTGLPKGVMLTHKGLVTS++QQVDGENPNLYY S+DV+LCVLP+FH
Sbjct: 179 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVSQQVDGENPNLYYSSDDVVLCVLPLFH 238
Query: 239 IYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKY 298
IYSLNSVLLCGLRA A+ILLM KF+I + L+ K++V++AP+VPP VLAIAK P+LDKY
Sbjct: 239 IYSLNSVLLCGLRAGAAILLMQKFEIVSLLELMQKHRVSVAPIVPPTVLAIAKFPDLDKY 298
Query: 299 DLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKS 358
DL SIRVLKSGGAPLGKELEDTVRAKFP LGQGYGMTEAGPVLTM L+FAKEP +VK
Sbjct: 299 DLGSIRVLKSGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKEPFEVKP 358
Query: 359 GACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLH 418
G CGTVVRNAE+KIVDP+ +SLPRN PGEICIRG QIMKGYLN+PEATR TIDK+GWLH
Sbjct: 359 GGCGTVVRNAELKIVDPETGASLPRNHPGEICIRGHQIMKGYLNDPEATRTTIDKQGWLH 418
Query: 419 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGE 478
TGDIGFIDDD+ELFIVDRLKELIKYKGFQVAPAELEA++++HP ISD AVVPM D+AAGE
Sbjct: 419 TGDIGFIDDDEELFIVDRLKELIKYKGFQVAPAELEALLVTHPNISDAAVVPMKDDAAGE 478
Query: 479 VPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKL 538
VPVAFVV GS TED+IK+F+SKQVVFYKRI RVFFI+AIPKSPSGKILRK+LRAKL
Sbjct: 479 VPVAFVVSPKGS-QITEDEIKQFISKQVVFYKRIKRVFFIEAIPKSPSGKILRKELRAKL 537
Query: 539 AAGVPN 544
AAG N
Sbjct: 538 AAGFAN 543
>pir||T02074 4-coumarate-CoA ligase (EC 6.2.1.12) - common tobacco
gi|1237183|dbj|BAA07828.1| 4-coumarate:coenzyme A ligase
[Nicotiana tabacum]
Length = 542
Score = 820 bits (2118), Expect = 0.0
Identities = 408/547 (74%), Positives = 469/547 (85%), Gaps = 8/547 (1%)
Query: 1 MTIQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQL 60
M T ++IF+SKLPDIYIP HLPLHSYCFEN+S++ SRPCLIN +IYTY DV+L
Sbjct: 1 MEKDTKHGDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVEL 60
Query: 61 TAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQA 120
+++KVA+GL+K GIQQ D IM+LLPN PEFVFAF+GAS+ GAI T ANP FT+AE+ KQ
Sbjct: 61 SSRKVAAGLHKQGIQQKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTAAEVVKQV 120
Query: 121 KASNTKLLVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKV 177
KAS K++VTQAC+ +KVKD NVK++ +DS+PE +HFS L QAD++++ EV+
Sbjct: 121 KASGAKIIVTQACHVNKVKDYALENNVKIICIDSAPE--GCLHFSVLTQADEHDIPEVE- 177
Query: 178 NIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMF 237
I+PDDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGEN NLY HSEDV+LCVLP+F
Sbjct: 178 -IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENRNLYIHSEDVLLCVLPLF 236
Query: 238 HIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDK 297
HIYSLNSVLLCGLR A+IL+M KFDI F L+ YKVTI P VPPIVLAIAKSP +D
Sbjct: 237 HIYSLNSVLLCGLRVGAAILIMQKFDIVPFLELIQNYKVTIGPFVPPIVLAIAKSPMVDD 296
Query: 298 YDLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVK 357
YDLSS+R + SG APLGKELEDTVRAKFP AKLGQGYGMTEAGPVL MCL+FAKEP ++K
Sbjct: 297 YDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIK 356
Query: 358 SGACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWL 417
SGACGTVVRNAEMKIVDP+ +SLPRNQ GEICIRGDQIMKGYLN+PEAT TIDKEGWL
Sbjct: 357 SGACGTVVRNAEMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWL 416
Query: 418 HTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAG 477
+TGDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEA++L+HP SD AVVPM DE A
Sbjct: 417 YTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPTFSDAAVVPMKDEQAE 476
Query: 478 EVPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAK 537
EVPVAFVVRS+GS TED++K F+SKQV+FYKRI RVFF+DA+PKSPSGKILRKDLRAK
Sbjct: 477 EVPVAFVVRSSGS-TITEDEVKDFISKQVIFYKRIKRVFFVDAVPKSPSGKILRKDLRAK 535
Query: 538 LAAGVPN 544
LAAG+PN
Sbjct: 536 LAAGLPN 542
>gb|AAF91309.1| 4-coumarate:coA ligase 2 [Rubus idaeus]
Length = 544
Score = 820 bits (2118), Expect = 0.0
Identities = 405/541 (74%), Positives = 470/541 (86%), Gaps = 7/541 (1%)
Query: 7 EQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVA 66
+ E IF+SKLPDIYIP HLPLH+YCFEN+S++ RPCLIN T E +TY +V+LT+++VA
Sbjct: 8 DHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELTSRRVA 67
Query: 67 SGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTK 126
+GL+KLGIQQ DV+M+LL NCPEF FAFLGAS+ GA+ T ANPF+T AE+AKQAKASN K
Sbjct: 68 AGLDKLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQAKASNAK 127
Query: 127 LLVTQACYYDKVKD---LENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDD 183
L++TQ+ Y DKVKD L +VK++ VD + ED +HFSEL+ AD++E VK+N PDD
Sbjct: 128 LIITQSAYVDKVKDFAKLNDVKVMCVDETSSEDV-LHFSELMSADESETPAVKIN--PDD 184
Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLN 243
VVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLY+H EDVILCVLP+FHIYSLN
Sbjct: 185 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLN 244
Query: 244 SVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSI 303
SV LCGLR A+IL+M KF+IN LV K KVTIAP VPPIVL+IAK P+L +YDLSSI
Sbjct: 245 SVFLCGLRVGAAILIMQKFEINKLLELVEKEKVTIAPFVPPIVLSIAKCPDLHRYDLSSI 304
Query: 304 RVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGT 363
R++ SG AP+GKELEDTVRAK P AKLGQGYGMTEAGPVL+MCL+FAKEP ++KSGACGT
Sbjct: 305 RMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYEIKSGACGT 364
Query: 364 VVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIG 423
VVRNAEMKI+DP + SLPRNQ GEICIRG QIMKGYLN+PEAT TIDKEGWLHTGDIG
Sbjct: 365 VVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENTIDKEGWLHTGDIG 424
Query: 424 FIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAF 483
+IDDDDELFIVDRLKELIKYKGFQVAPAELEA+++SHP +SD AVV M DEAAGEVPVAF
Sbjct: 425 YIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGEVPVAF 484
Query: 484 VVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAGVP 543
VVRSNGS +EDDIK+++SKQVVFYKRI++VFF D IPK+PSGKILRKDLRA+LAAG+P
Sbjct: 485 VVRSNGS-KISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLAAGLP 543
Query: 544 N 544
N
Sbjct: 544 N 544
>gb|AAC24503.1| 4-coumarate:CoA ligase [Populus tremuloides] gi|7437852|pir||T08074
4-coumarate-CoA ligase (EC 6.2.1.12) - quaking aspen
Length = 535
Score = 816 bits (2108), Expect = 0.0
Identities = 402/534 (75%), Positives = 470/534 (87%), Gaps = 8/534 (1%)
Query: 8 QELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQLTAQKVAS 67
QE IF+SKLPDIYIPK+LPLHSY ENLSK+ S+PCLIN ++YTY DV+LTA++VAS
Sbjct: 4 QEFIFRSKLPDIYIPKNLPLHSYVLENLSKHSSKPCLINGANGDVYTYADVELTARRVAS 63
Query: 68 GLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQAKASNTKL 127
GLNK+GIQQGDVIM+ LP+ PEFV AFLGAS RGA++TAANPF T AE+AK AKAS KL
Sbjct: 64 GLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAMITAANPFSTPAELAKHAKASRAKL 123
Query: 128 LVTQACYYDKVKDL---ENVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKVNIKPDDV 184
L+TQACYY+KVKD +VK++ VDS+P D F QAD+NE+ +V +I PDDV
Sbjct: 124 LITQACYYEKVKDFARESDVKVMCVDSAP--DGASLFRAHTQADENEVPQV--DISPDDV 179
Query: 185 VALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMFHIYSLNS 244
VALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+HSEDVILCVLPMFHIY+LNS
Sbjct: 180 VALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVILCVLPMFHIYALNS 239
Query: 245 VLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDKYDLSSIR 304
++LCGLR ASIL+MPKF+I + GL+ KYKV+IAPVVPP+++AIAKSP+LDK+DLSS+R
Sbjct: 240 MMLCGLRVGASILIMPKFEIGSLLGLIEKYKVSIAPVVPPVMMAIAKSPDLDKHDLSSLR 299
Query: 305 VLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVKSGACGTV 364
++KSGGAPLGKELEDTVRAKFP+A+LGQGYGMTEAGPVL MCL+FAKEP D+K GACGTV
Sbjct: 300 MIKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGACGTV 359
Query: 365 VRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWLHTGDIGF 424
VRNAEMKIVDP+ SLPRNQPGEICIRGDQIMKGYLN+PEAT TIDKEGWLHTGDIG+
Sbjct: 360 VRNAEMKIVDPETGVSLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKEGWLHTGDIGY 419
Query: 425 IDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAGEVPVAFV 484
IDDDDELFIVDRLKELIKYKGFQVAP ELEA++++HP+ISD AVV + DE AGEVPVAFV
Sbjct: 420 IDDDDELFIVDRLKELIKYKGFQVAPTELEALLIAHPEISDAAVVGLKDEDAGEVPVAFV 479
Query: 485 VRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKL 538
V+S S TED+IK+++SKQV+FYKRI RVFFI+AIPK+PSGKILRK+L+ KL
Sbjct: 480 VKSEKS-QATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLKEKL 532
>gb|AAP68991.1| 4-coumarate:coenzyme A ligase 2 [Salvia miltiorrhiza]
Length = 540
Score = 811 bits (2094), Expect = 0.0
Identities = 401/545 (73%), Positives = 470/545 (85%), Gaps = 8/545 (1%)
Query: 1 MTIQTMEQELIFKSKLPDIYIPKHLPLHSYCFENLSKYGSRPCLINAPTAEIYTYYDVQL 60
M + TM +E++F+SKLPDIYIPKHLPLHSYCFEN+SK+ SRPC+IN T ++YTY +V++
Sbjct: 1 MEVPTMPEEIVFRSKLPDIYIPKHLPLHSYCFENISKFSSRPCIINGATGDVYTYEEVEM 60
Query: 61 TAQKVASGLNKLGIQQGDVIMVLLPNCPEFVFAFLGASFRGAIMTAANPFFTSAEIAKQA 120
TA+KVASGL+++GIQQG+ IM+LLPN PE++FAFLGAS+ GA+ T ANPFFT AE+ KQA
Sbjct: 61 TARKVASGLSQVGIQQGETIMLLLPNTPEYIFAFLGASYIGAVSTMANPFFTPAEVIKQA 120
Query: 121 KASNTKLLVTQACYYDKVKDLE---NVKLVFVDSSPEEDNHMHFSELIQADQNEMEEVKV 177
KAS KL++TQACY DKV+D K+V +D+ P + FSEL AD+ EM VK+
Sbjct: 121 KASAAKLIITQACYVDKVRDYAAEAGAKVVCIDAPPA--GCLTFSELTAADEREMPAVKI 178
Query: 178 NIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHSEDVILCVLPMF 237
+ P+D VALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLY HSEDV+LCVLP+F
Sbjct: 179 H--PEDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLF 236
Query: 238 HIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKYKVTIAPVVPPIVLAIAKSPELDK 297
HIYSLNSVLLCGLR A+IL+M KF+I F L+ +YKVTI P VPPIVLAIAKSP + K
Sbjct: 237 HIYSLNSVLLCGLRVGAAILIMQKFEIVPFLDLMQRYKVTIGPFVPPIVLAIAKSPLVAK 296
Query: 298 YDLSSIRVLKSGGAPLGKELEDTVRAKFPKAKLGQGYGMTEAGPVLTMCLSFAKEPIDVK 357
YDLSS+R++ SG APLGKELED+VR KFP AKLGQGYGMTEAGPVL+MCL+FAKEP ++K
Sbjct: 297 YDLSSVRMVMSGAAPLGKELEDSVRTKFPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIK 356
Query: 358 SGACGTVVRNAEMKIVDPQNDSSLPRNQPGEICIRGDQIMKGYLNNPEATRETIDKEGWL 417
SGACGTVVRNAEMKI+DPQ SL RNQ GEICIRGDQIMKGYLN+PE+T+ TID++GWL
Sbjct: 357 SGACGTVVRNAEMKIIDPQTGVSLGRNQSGEICIRGDQIMKGYLNDPESTKNTIDEDGWL 416
Query: 418 HTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAIILSHPQISDVAVVPMLDEAAG 477
HTGDIGFID DDELFIVDRLKE+IKYKGFQVAPAE+EA++L++P ISD AVV M DE AG
Sbjct: 417 HTGDIGFIDADDELFIVDRLKEIIKYKGFQVAPAEIEALLLNNPYISDAAVVSMQDEQAG 476
Query: 478 EVPVAFVVRSNGSIDTTEDDIKKFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAK 537
EVPVAFVVRSNGS TED+IK+F+SKQV+FYKRINRVFFIDAIPKSPSGKILRKDLRA+
Sbjct: 477 EVPVAFVVRSNGS-TITEDEIKQFISKQVIFYKRINRVFFIDAIPKSPSGKILRKDLRAR 535
Query: 538 LAAGV 542
LAA V
Sbjct: 536 LAAAV 540
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.319 0.137 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 903,300,006
Number of Sequences: 2540612
Number of extensions: 38307294
Number of successful extensions: 120867
Number of sequences better than 10.0: 5363
Number of HSP's better than 10.0 without gapping: 4346
Number of HSP's successfully gapped in prelim test: 1017
Number of HSP's that attempted gapping in prelim test: 96747
Number of HSP's gapped (non-prelim): 9403
length of query: 544
length of database: 863,360,394
effective HSP length: 133
effective length of query: 411
effective length of database: 525,458,998
effective search space: 215963648178
effective search space used: 215963648178
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 78 (34.7 bits)
Medicago: description of AC144503.3