BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140068.2 + phase: 0
(501 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC16945.1| putative pseudouridine synthase [Arabidopsis thal... 490 e-137
ref|XP_640538.1| hypothetical protein DDB0204564 [Dictyostelium ... 140 7e-32
gb|AAN18175.1| At1g76120/T23E18_5 [Arabidopsis thaliana] gi|2159... 117 1e-24
ref|XP_637536.1| hypothetical protein DDB0187011 [Dictyostelium ... 115 3e-24
gb|AAS53269.1| AFL105Cp [Ashbya gossypii ATCC 10895] gi|45198416... 112 3e-23
emb|CAH02024.1| unnamed protein product [Kluyveromyces lactis NR... 111 6e-23
ref|XP_624092.1| PREDICTED: similar to ENSANGP00000014976 [Apis ... 109 2e-22
ref|NP_974152.1| tRNA pseudouridine synthase family protein [Ara... 108 3e-22
ref|XP_635437.1| tRNA pseudouridylate synthase [Dictyostelium di... 104 7e-21
emb|CAF91572.1| unnamed protein product [Tetraodon nigroviridis] 101 5e-20
ref|XP_415090.1| PREDICTED: similar to tRNA pseudouridine syntha... 100 8e-20
ref|NP_062674.1| pseudouridine synthase 1 [Mus musculus] gi|4455... 100 2e-19
gb|AAH21446.1| Pseudouridine synthase 1 [Mus musculus] 100 2e-19
dbj|BAC40817.1| unnamed protein product [Mus musculus] 100 2e-19
ref|XP_222267.1| PREDICTED: similar to pseudouridine synthase 1 ... 99 2e-19
gb|AAH02901.1| PUS1 protein [Homo sapiens] gi|17939636|gb|AAH193... 99 3e-19
gb|AAL55876.1| unknown [Homo sapiens] 99 3e-19
gb|AAD21042.1| pseudouridine synthase 1 [Homo sapiens] 99 3e-19
gb|AAH86827.1| Zgc:103516 [Danio rerio] gi|56693277|ref|NP_00100... 98 5e-19
ref|XP_656284.1| tRNA pseudouridine synthase, putative [Entamoeb... 94 8e-18
>gb|AAC16945.1| putative pseudouridine synthase [Arabidopsis thaliana]
gi|3914486|sp|O22928|PUSH_ARATH Putative tRNA
pseudouridine synthase (tRNA-uridine isomerase) (tRNA
pseudouridylate synthase) gi|15227735|ref|NP_180591.1|
tRNA pseudouridine synthase family protein [Arabidopsis
thaliana]
Length = 510
Score = 490 bits (1262), Expect = e-137
Identities = 251/452 (55%), Positives = 320/452 (70%), Gaps = 39/452 (8%)
Query: 51 WQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKI 110
W+ +RKKKVV+RI Y GT+YRGLQ+QRD S+ T+E ELE AI+KAGGIR+SN GDL KI
Sbjct: 57 WESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKI 116
Query: 111 GWARSSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQ 170
GWARSSRTDKGVHSLAT ++ KMEIPE +W +DP G LA ++ +LP +I+V S+LP+
Sbjct: 117 GWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSN 176
Query: 171 RSFDPRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSR 230
R FDPR+EC LRKYSYLLP D++GI++ ++DEIDYH+++FN+ILKEFEG +PFHNYT R
Sbjct: 177 RRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQR 236
Query: 231 SRYRR-----------HIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQ 279
SRYRR PR P K E + E +EEE +VD
Sbjct: 237 SRYRRKSEQKIKQRNGRPPREPKSIKASESEFREENHIEIGEEEEEKEVD---------- 286
Query: 280 NQKSSGTSDSCEPVIETGNKGNLHDQSSSSVV-RAKWLYEPDEADRLNASHFRRVFRCSS 338
G SD E V+ D +S V RAKWLYEPDE D+++ +HFR+VFRC S
Sbjct: 287 -----GESD--EHVVTP-------DSDNSQVYSRAKWLYEPDETDKISGAHFRKVFRCRS 332
Query: 339 GKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPL 398
GKLE S+G ++EI I GESFMLHQIRKM+ TAVAVKR+LLPRDII LSL+KF+RI+LPL
Sbjct: 333 GKLENSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPL 392
Query: 399 APSEVLLLRGNSFSMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQVSKFLDPSR 458
APSEVL+LRGNSF +R P RP M+ ESEE+ K +++FYR+VM+PQVS FLD +
Sbjct: 393 APSEVLILRGNSFEVRRLP---ERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEK 449
Query: 459 VPWVEWIEKFDAHTSIPNDQLDEVRKARNLWK 490
PW EW++ D + + +++L++VRK WK
Sbjct: 450 SPWKEWVDHLDRNDGLIDEELEDVRKGWEEWK 481
>ref|XP_640538.1| hypothetical protein DDB0204564 [Dictyostelium discoideum]
gi|60468568|gb|EAL66571.1| hypothetical protein
DDB0204564 [Dictyostelium discoideum]
Length = 504
Score = 140 bits (354), Expect = 7e-32
Identities = 115/429 (26%), Positives = 204/429 (46%), Gaps = 59/429 (13%)
Query: 55 RKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWAR 114
+K V M Y GT Y GL Q D S +E +E +F+ G I SN+G+L+++ W+
Sbjct: 87 KKLSVAMVFGYDGTGYYGLGYQVDIKYPS-IENLIEKVLFRCGHILPSNVGELKRLKWSH 145
Query: 115 SSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
SSRTDKGVHSLAT+++ + + +N++ D + +N+YLP I++ S RSF
Sbjct: 146 SSRTDKGVHSLATVISCFLVVDDNTFKND-INQTFIDTINNYLPSSIRLFSACKVSRSFQ 204
Query: 175 PRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSRSRYR 234
RK+ + R Y+Y++P++ + +N +ID E N IL+ F G + +HN+TS+
Sbjct: 205 ARKKGIERSYNYMVPSEYL------NNIKID----EMNKILQNFIGINSYHNFTSKRNTY 254
Query: 235 RHIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSDSCEPVI 294
+H K ++ED DEEE + DE +E +N ++ ++
Sbjct: 255 LSNNNNKNHEK-------DEKDEEDGDEEEE-EDDE-----VEVENSNNNNSNYRL---- 297
Query: 295 ETGNKGNLHDQSSSSVVRAKWLYEPDEADRLNASHFRRVFRCSSGKLEKSMGHSYIEIYI 354
N+ L +S+++ K Y +E + G + + I
Sbjct: 298 ---NEKLLFRNNSTNIRNIKSFYVENEIIEIK-------------------GKGWYKFVI 335
Query: 355 EGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLLRGNSFSMR 414
GESF+L+QIR+MV +++ + + + LSL I +P AP L L F+++
Sbjct: 336 IGESFILYQIRRMVGFFLSIVKGYSTVEALELSLKSPFSICVPTAPPFPLYL----FNVK 391
Query: 415 TKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQVSKFLDPSRVPWVEWIEKFDAHTSI 474
P + + E I +F +S ++P+ ++ ++ + E+ + +
Sbjct: 392 FDPNSNVE---KLFSNPEPIQFKQFNFLQSQLIPRFNE-IEFQTNQFKEFFNYLENSHNF 447
Query: 475 PNDQLDEVR 483
D +D++R
Sbjct: 448 NLDDIDQLR 456
>gb|AAN18175.1| At1g76120/T23E18_5 [Arabidopsis thaliana]
gi|21592498|gb|AAM64448.1| unknown [Arabidopsis
thaliana] gi|21703099|gb|AAM74492.1| At1g76120/T23E18_5
[Arabidopsis thaliana] gi|18411027|ref|NP_565126.1| tRNA
pseudouridine synthase family protein [Arabidopsis
thaliana] gi|6573728|gb|AAF17648.1| T23E18.5
[Arabidopsis thaliana]
Length = 463
Score = 117 bits (292), Expect = 1e-24
Identities = 105/377 (27%), Positives = 167/377 (43%), Gaps = 53/377 (14%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
+KV + A+ G Y+G+Q T+E ELE A+F AG + ES G + +ARS+
Sbjct: 22 QKVAIIFAFCGVGYQGMQKNP---GAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 78
Query: 117 RTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPR 176
RTDKGV ++ +V+ + + DP GF N +NS LP I++ SF +
Sbjct: 79 RTDKGVSAVGQVVSGRFIV-------DPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 129
Query: 177 KECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSRSRYRRH 236
K C R+Y YLLP + SH + + + + +K FE S R
Sbjct: 130 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFEC----------SERGRK 179
Query: 237 IPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSDSCEPVIET 296
IP G + + ++ D + + AL +I+ + SS + C +E
Sbjct: 180 IPPGLVGKWKG---TNFGTKSLDFQSDISSNNSSALRSDIKIE-ALSSNLAGLCSVDVEV 235
Query: 297 G----NKGNLHDQSSSSVVRAKWLYEPDEADRL-----------NASHF----------- 330
G + L+ SS + V++K+ Y E +R N +F
Sbjct: 236 GRIQEDSCKLNTNSSETKVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTA 295
Query: 331 -RRVFRCSSGKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLS 389
R++ ++ + G +I+ + G+SFMLHQIRKM+ AVA+ R +I + S
Sbjct: 296 NRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFS 355
Query: 390 KFSRIILPLAPSEVLLL 406
K I +P+AP L L
Sbjct: 356 KDVNITVPMAPEVGLYL 372
>ref|XP_637536.1| hypothetical protein DDB0187011 [Dictyostelium discoideum]
gi|60465972|gb|EAL64039.1| hypothetical protein
DDB0187011 [Dictyostelium discoideum]
Length = 615
Score = 115 bits (288), Expect = 3e-24
Identities = 118/470 (25%), Positives = 204/470 (43%), Gaps = 59/470 (12%)
Query: 35 ECFRYCSFSSTPQSYSWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIF 94
+ +C+ + + + KK + + AY G+ Y G D +E +E ++
Sbjct: 126 DLIEFCNTHNQEIEQNLKDKSKKPMALCFAYNGSKYYGSSYSFDIR-FPAIENLIEKSLI 184
Query: 95 KAGGIRESNLGDLEKIGWARSSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFA---LAN 151
+ G I N+G+L++I +RSSRTDKGVHS++T+++ + I + + FG +
Sbjct: 185 RNGHIALENIGNLKRIKLSRSSRTDKGVHSVSTVISAFLLIDKETERLAQFGTPPQYFID 244
Query: 152 YVNSYLPCDIKVISILPAQRSFDPRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEF 211
+N YLP DI++IS R+F R R Y+YL+P D H + ID
Sbjct: 245 NINRYLPSDIRLISATKPSRTFRARPASKSRVYNYLVPMD------HLKDISID----RM 294
Query: 212 NDILKEFEGGHPFHNYTSRSRYRRHIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEA 271
N IL F G FHNYT Y+R + K ++ E E++D+EE+ + D+
Sbjct: 295 NSILSNFIGTSSFHNYT----YQRFTYLDSNTLKKLSKINDTQDENENNDDEEDEEEDDE 350
Query: 272 LTGNIECQNQKSSGTSDSCEPVIETGNKGNLH-DQSSSSVVRAKWLYEPDEADRLNASHF 330
GN + E + + NK N+ D+ + S E R N +
Sbjct: 351 EDGN----DNTKIDIEKEVEELEKLENKKNIEIDRLAES-----------ELFRKNPVNV 395
Query: 331 RRVFRCSSGKLEKSM----GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITL 386
R + + +EK G + + G+SF+++QIRKM+ ++V + + L
Sbjct: 396 RTIM---NFYVEKETVLFEGKQWYRFVVVGDSFLMYQIRKMMGFFLSVVKGYQNEIGLQL 452
Query: 387 SLSKFSRIILPLAPSEVLLLRGNSFSMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVM 446
SL I P++P L L +F V+R ++ ++F S +
Sbjct: 453 SLKSPFSICSPISPPFPLFLYDVNFPREKILLPVSR-------------QIQNEFLNSKL 499
Query: 447 LPQVSKFLDPSRVPWVEWIEKFDAHTSIPNDQLDEVRKARNLWKENFDSV 496
+P + K + + +F S N +LD++ + L KE + +
Sbjct: 500 MPHLHKIENET-----NQFSEFFNQLSTFNFRLDDIDQLIILNKEFHEKI 544
>gb|AAS53269.1| AFL105Cp [Ashbya gossypii ATCC 10895] gi|45198416|ref|NP_985445.1|
AFL105Cp [Eremothecium gossypii]
Length = 528
Score = 112 bits (280), Expect = 3e-23
Identities = 101/355 (28%), Positives = 158/355 (44%), Gaps = 30/355 (8%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+KV + + Y GT Y G+Q T+E EL A KAG I +N DL+K G+ R+
Sbjct: 66 KRKVAVMVGYCGTGYHGMQYNPPNR---TIEAELFEAFVKAGAISRANSTDLKKNGFMRA 122
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGVH+ +++ K+ I EDP A+ + +N +LP I+V I ++FD
Sbjct: 123 ARTDKGVHAGGNVISLKLII------EDP---AIKDKINEHLPPGIRVWGISRVNKAFDC 173
Query: 176 RKECVLRKYSYLLPA-DIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSR---S 231
RK C R Y YLLP IG + ++ +++ + E P + S
Sbjct: 174 RKLCGSRWYEYLLPTYSFIGPKPNT-------YLARTIEQCGEAASEKPDRDQESLDFWE 226
Query: 232 RYRRHIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSDSCE 291
+R+ + + + + I++ +ED DE +E +++S S +
Sbjct: 227 AFRKAVDEKFTQEEQDAIVNYVAPSKEDFDENSGLYQKVKQYKQMENAHRRSYRVSSAKL 286
Query: 292 PVIETGNKGNLHDQSSSSVVRAKWLYEPDEADRLNASHFRRVFRCSSGKLEKSMGHSYIE 351
K L + + K +P + R F K E ++
Sbjct: 287 ARFREAMKQYLGPHNFHNYTLGKDFKDPSTVRFMKDITVSRPFVIGEMKTE------WVS 340
Query: 352 IYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLL 406
I I G+SFMLHQIRKM++ A V R + I S +I +P AP+ LLL
Sbjct: 341 IKIHGQSFMLHQIRKMISMATLVARCNCSPERIAQSYGP-QKINIPKAPALGLLL 394
>emb|CAH02024.1| unnamed protein product [Kluyveromyces lactis NRRL Y-1140]
gi|50303379|ref|XP_451631.1| unnamed protein product
[Kluyveromyces lactis]
Length = 553
Score = 111 bits (277), Expect = 6e-23
Identities = 98/353 (27%), Positives = 161/353 (44%), Gaps = 27/353 (7%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+KV + I Y GT Y G+Q + T+EKEL A +AG I ++N DL+K G+ R+
Sbjct: 98 KRKVAVMIGYCGTGYHGMQYNPPND---TIEKELFEAFVRAGAISKANSTDLKKNGFQRA 154
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGVH+ +++ K+ I EDP + +N+ LP I+V I ++FD
Sbjct: 155 ARTDKGVHAGGNVISLKLII------EDP---EVKEKINNELPDQIRVWDISRVNKAFDC 205
Query: 176 RKECVLRKYSYLLPA-DIIGIQ-SHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSRSRY 233
RK C R Y YLLP +IG + S +EI+ E +++++ F S
Sbjct: 206 RKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAE 265
Query: 234 RRHIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSDSCEPV 293
+ E ++A+ ++ DE +E +++++ S
Sbjct: 266 SKFTKEEL------EEIAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRISKERLDR 319
Query: 294 IETGNKGNLHDQSSSSVVRAKWLYEPDEADRLNASHFRRVFRCSSGKLEKSMGHSYIEIY 353
+ K + + K +P +A F + S + ++ I
Sbjct: 320 FRSALKQYEGTFNFHNFTLGKDFNDP------SAKRFMKQITVSEPFVIGEAKTEWVSIK 373
Query: 354 IEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLL 406
I G+SFMLHQIRKM++ A + R P + I + + +I +P AP+ LLL
Sbjct: 374 IHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQ-EKINIPKAPALGLLL 425
>ref|XP_624092.1| PREDICTED: similar to ENSANGP00000014976 [Apis mellifera]
Length = 352
Score = 109 bits (273), Expect = 2e-22
Identities = 64/183 (34%), Positives = 104/183 (55%), Gaps = 19/183 (10%)
Query: 55 RKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWAR 114
+KKK VM + Y G NY G+Q+ R + T+E L SA+ KA I ++ DL++I + R
Sbjct: 31 KKKKFVMVLGYLGKNYSGMQINR---GIKTIEGSLLSALLKADFITKNEFEDLKEIKFQR 87
Query: 115 SSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
++RTDKGV ++ +V+ +++P + ED +N +LP DIKV I R+F+
Sbjct: 88 AARTDKGVSAVRQIVS--LQLPNHVNKED---------INKHLPKDIKVFDIKRVTRNFN 136
Query: 175 PRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTS 229
+ +C R Y Y+LP + + + ++ ++ + N+ILK FEG H FHN+TS
Sbjct: 137 SKNKCDARTYRYVLPTFALASEDPNGKPYNEFRLNSDMLDKLNEILKLFEGTHNFHNFTS 196
Query: 230 RSR 232
+ R
Sbjct: 197 KIR 199
Score = 50.4 bits (119), Expect = 1e-04
Identities = 29/118 (24%), Positives = 62/118 (51%), Gaps = 5/118 (4%)
Query: 334 FRCSSGKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSR 393
FRC + +M ++ + ++G+SFMLHQIRKMV+ + + R ++ D + + S +
Sbjct: 212 FRCIETFIANNM--EFVVLEVQGQSFMLHQIRKMVSLIIGICRNIITIDFVKDAFS-LEK 268
Query: 394 IILPLAPSEVLLLRGNSFSMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQVS 451
+ +P+AP L L ++ + + +++ E N+ ++ FY+ +L ++
Sbjct: 269 VDIPIAPGLGLSLHFVHYTYYNE--RYGKDGFHEMLDWNECNEEIEKFYKEYILKYIT 324
>ref|NP_974152.1| tRNA pseudouridine synthase family protein [Arabidopsis thaliana]
Length = 356
Score = 108 bits (271), Expect = 3e-22
Identities = 99/361 (27%), Positives = 159/361 (43%), Gaps = 53/361 (14%)
Query: 57 KKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARSS 116
+KV + A+ G Y+G+Q T+E ELE A+F AG + ES G + +ARS+
Sbjct: 22 QKVAIIFAFCGVGYQGMQKNP---GAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 78
Query: 117 RTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPR 176
RTDKGV ++ +V+ + + DP GF N +NS LP I++ SF +
Sbjct: 79 RTDKGVSAVGQVVSGRFIV-------DPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 129
Query: 177 KECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSRSRYRRH 236
K C R+Y YLLP + SH + + + + +K FE S R
Sbjct: 130 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFEC----------SERGRK 179
Query: 237 IPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSDSCEPVIET 296
IP G + + ++ D + + AL +I+ + SS + C +E
Sbjct: 180 IPPGLVGKWKG---TNFGTKSLDFQSDISSNNSSALRSDIKIE-ALSSNLAGLCSVDVEV 235
Query: 297 G----NKGNLHDQSSSSVVRAKWLYEPDEADRL-----------NASHF----------- 330
G + L+ SS + V++K+ Y E +R N +F
Sbjct: 236 GRIQEDSCKLNTNSSETKVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTA 295
Query: 331 -RRVFRCSSGKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLS 389
R++ ++ + G +I+ + G+SFMLHQIRKM+ AVA+ R +I + S
Sbjct: 296 NRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFS 355
Query: 390 K 390
K
Sbjct: 356 K 356
>ref|XP_635437.1| tRNA pseudouridylate synthase [Dictyostelium discoideum]
gi|60463746|gb|EAL61924.1| tRNA pseudouridylate synthase
[Dictyostelium discoideum]
Length = 596
Score = 104 bits (259), Expect = 7e-21
Identities = 67/195 (34%), Positives = 94/195 (47%), Gaps = 28/195 (14%)
Query: 55 RKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWAR 114
RKKKV + + Y+GTNY G+Q L TVE+ELE A+F+ GGI N+ +K+ W R
Sbjct: 138 RKKKVAIILGYSGTNYAGMQKNP---GLKTVEEELEHALFRTGGISPDNMYYQQKVDWIR 194
Query: 115 SSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
+RTDKGV ++ +V+ KME+ + G +A +N LP DI V I+ SF
Sbjct: 195 CARTDKGVSAIRNVVSLKMEMGPPDFE----GMKIA--LNKELPKDITVFHIMRVNNSFH 248
Query: 175 PRKECVLRKYSYLLPADIIGIQSHSSNDEI-------------------DYHMSEFNDIL 215
+ R Y Y+ P + + N I +S N +L
Sbjct: 249 AKNAVDARSYIYVTPTHVFEPKFSKKNKSIVEEAANGGENNTQPPWKFTSDTLSILNKVL 308
Query: 216 KEFEGGHPFHNYTSR 230
+ G H FHNYTSR
Sbjct: 309 SFYLGNHRFHNYTSR 323
Score = 55.1 bits (131), Expect = 5e-06
Identities = 49/173 (28%), Positives = 82/173 (47%), Gaps = 24/173 (13%)
Query: 326 NASHFRRV--FRCSSGKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDI 383
+ S FR++ F CS + + G + I + GESFMLHQIRKM+ ++ RK + D
Sbjct: 328 DVSVFRKIHSFVCSEPFVLE--GVEMVSINVVGESFMLHQIRKMIGCLISFIRKGMFEDC 385
Query: 384 ITL------------SLSKFSRIILPLAPSEVLLLRGNSFSMRTKPGNVTRPEMQTIVES 431
T S + + + LP+AP LLL F+ K + E++ +ES
Sbjct: 386 ETRDQAFDQIKAYINSTFEHTPLNLPMAPGAGLLLDYCIFNEWNKNNSSIHGELK--IES 443
Query: 432 EEINKVVDDFYRSVMLPQVSKFLDPSRVPWVEWIEKFDAHTSIPNDQLDEVRK 484
+++ + YR + L L+ ++ + WIEK +P + +DE+ K
Sbjct: 444 DQVEEFKKTIYREIAL------LESTKKEFKNWIEKNLDPYPLPYNLMDEIIK 490
>emb|CAF91572.1| unnamed protein product [Tetraodon nigroviridis]
Length = 375
Score = 101 bits (252), Expect = 5e-20
Identities = 61/209 (29%), Positives = 109/209 (51%), Gaps = 17/209 (8%)
Query: 47 QSYSWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGD 106
QS + + KKKV + +AY+G Y G+Q + T+E +L +A+ K+G I E++ D
Sbjct: 65 QSQEDRRYPKKKVALLLAYSGKGYYGMQRNPGSSEFRTIEDDLVAALVKSGCIPENHAED 124
Query: 107 LEKIGWARSSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISI 166
++K+ + R +RTDKGV + +V+ K+ + ++ LA +N +LP I+V+ +
Sbjct: 125 MKKMSFQRCARTDKGVSAAGQVVSLKLWLIDD----------LAEKINQHLPPQIRVLGV 174
Query: 167 LPAQRSFDPRKECVLRKYSYLLPADIIGIQ-SHSSNDEIDYH-----MSEFNDILKEFEG 220
+ F+ + C R Y+Y+LP + S +++D + + N + ++G
Sbjct: 175 KRVTQGFNSKNNCDARTYAYMLPTVAFAPKDSDAASDAASFRLQPETLQRVNRLFALYKG 234
Query: 221 GHPFHNYTSRSRYRRHIPRR-PSHSKCGE 248
H FHN+TS+ RR +H CGE
Sbjct: 235 THNFHNFTSQKAPNDPSARRYVTHVCCGE 263
Score = 34.3 bits (77), Expect = 9.4
Identities = 14/23 (60%), Positives = 18/23 (77%)
Query: 352 IYIEGESFMLHQIRKMVATAVAV 374
I + G+SFMLHQIRKM+ +AV
Sbjct: 275 ITVRGQSFMLHQIRKMIGLVIAV 297
>ref|XP_415090.1| PREDICTED: similar to tRNA pseudouridine synthase A
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase) [Gallus gallus]
Length = 409
Score = 100 bits (250), Expect = 8e-20
Identities = 56/203 (27%), Positives = 106/203 (51%), Gaps = 25/203 (12%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ ++G I E++ D++K+ + R
Sbjct: 63 KRKIVLLMAYSGKGYHGMQRNLGSSQFKTIEDDLVSALVRSGCIPENHGEDMKKMSFQRC 122
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + +N++LP I+++ + F+
Sbjct: 123 ARTDKGVSAAGQIVSLKVRLIDD----------ILEKINNHLPSHIRILGLKRVTGGFNS 172
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEID----YHMSEFNDILKEFEGGHPFHNYTSRS 231
+ +C R Y+Y+LP + H +E + + N +L ++G H FHN+TS+
Sbjct: 173 KNKCDARTYAYMLPTFAFAHKDHDVQEEAHRLSRETLEKVNSLLARYKGTHNFHNFTSQK 232
Query: 232 RYRRHIPRRPSHSK------CGE 248
P+ PS + CGE
Sbjct: 233 G-----PKDPSARRYIMEMYCGE 250
Score = 42.7 bits (99), Expect = 0.026
Identities = 22/61 (36%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKM+ +A+ + P II S + ++ +P AP L+
Sbjct: 256 GIEFAVIKVKGQSFMMHQIRKMIGLVIAIVKGYAPESIIERSWGE-EKVDVPKAPGLGLV 314
Query: 406 L 406
L
Sbjct: 315 L 315
>ref|NP_062674.1| pseudouridine synthase 1 [Mus musculus] gi|4455033|gb|AAD21041.1|
pseudouridine synthase 1 [Mus musculus]
gi|21707763|gb|AAH34359.1| Pseudouridine synthase 1 [Mus
musculus] gi|8134771|sp|Q9WU56|TRUA_MOUSE tRNA
pseudouridine synthase A (tRNA-uridine isomerase I)
(tRNA pseudouridylate synthase I)
gi|11464734|gb|AAG35308.1| pseudouridine synthase 1 [Mus
musculus]
Length = 393
Score = 99.8 bits (247), Expect = 2e-19
Identities = 57/190 (30%), Positives = 101/190 (53%), Gaps = 16/190 (8%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ +AG I E++ D+ K+ + R
Sbjct: 49 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 108
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + + +NS+LP I+++ + F+
Sbjct: 109 ARTDKGVSAAGQVVSLKVWLIDD----------ILDKINSHLPSHIRILGLKRVTGGFNS 158
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ +C R Y Y+LP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 159 KNKCDARTYCYMLPTFAFAHKDRDVQDE-SYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 217
Query: 231 SRYRRHIPRR 240
R RR
Sbjct: 218 KGPREPSARR 227
Score = 42.4 bits (98), Expect = 0.035
Identities = 22/61 (36%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S + ++ +P AP L+
Sbjct: 242 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGE-EKVDVPKAPGLGLV 300
Query: 406 L 406
L
Sbjct: 301 L 301
>gb|AAH21446.1| Pseudouridine synthase 1 [Mus musculus]
Length = 393
Score = 99.8 bits (247), Expect = 2e-19
Identities = 57/190 (30%), Positives = 101/190 (53%), Gaps = 16/190 (8%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ +AG I E++ D+ K+ + R
Sbjct: 49 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 108
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + + +NS+LP I+++ + F+
Sbjct: 109 ARTDKGVSAAGQVVSLKVWLIDD----------ILDKINSHLPSHIRILGLKRVTGGFNS 158
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ +C R Y Y+LP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 159 KNKCDARTYCYMLPTFAFAHKDRDVQDE-SYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 217
Query: 231 SRYRRHIPRR 240
R RR
Sbjct: 218 KGPREPSARR 227
Score = 42.4 bits (98), Expect = 0.035
Identities = 22/61 (36%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S + ++ +P AP L+
Sbjct: 242 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGE-EKVDVPKAPGLGLV 300
Query: 406 L 406
L
Sbjct: 301 L 301
>dbj|BAC40817.1| unnamed protein product [Mus musculus]
Length = 393
Score = 99.8 bits (247), Expect = 2e-19
Identities = 57/190 (30%), Positives = 101/190 (53%), Gaps = 16/190 (8%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ +AG I E++ D+ K+ + R
Sbjct: 49 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 108
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + + +NS+LP I+++ + F+
Sbjct: 109 ARTDKGVSAAGQVVSLKVWLIDD----------ILDKINSHLPSHIRILGLKRVTGGFNS 158
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ +C R Y Y+LP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 159 KNKCDARTYCYMLPTFAFAHKDRDVQDE-SYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 217
Query: 231 SRYRRHIPRR 240
R RR
Sbjct: 218 KGPREPSARR 227
Score = 42.4 bits (98), Expect = 0.035
Identities = 22/61 (36%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S + ++ +P AP L+
Sbjct: 242 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGE-EKVDVPKAPGLGLV 300
Query: 406 L 406
L
Sbjct: 301 L 301
>ref|XP_222267.1| PREDICTED: similar to pseudouridine synthase 1 [Rattus norvegicus]
Length = 393
Score = 99.4 bits (246), Expect = 2e-19
Identities = 57/190 (30%), Positives = 101/190 (53%), Gaps = 16/190 (8%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ +AG I E++ D+ K+ + R
Sbjct: 49 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 108
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + + +NS+LP I+++ + F+
Sbjct: 109 ARTDKGVSAAGQVVSLKVWLIDD----------ILDKINSHLPSHIRILGLKRVTGGFNS 158
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ +C R Y Y+LP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 159 KNKCDARTYCYMLPTFAFAHKDRDVQDE-SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQ 217
Query: 231 SRYRRHIPRR 240
R RR
Sbjct: 218 KGPREPSARR 227
Score = 42.4 bits (98), Expect = 0.035
Identities = 22/61 (36%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S + ++ +P AP L+
Sbjct: 242 GLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGE-EKVDVPKAPGLGLV 300
Query: 406 L 406
L
Sbjct: 301 L 301
>gb|AAH02901.1| PUS1 protein [Homo sapiens] gi|17939636|gb|AAH19320.1|
Pseudouridylate synthase 1 [Homo sapiens]
gi|14550510|gb|AAH09505.1| Pseudouridylate synthase 1,
isoform 1 [Homo sapiens] gi|13376820|ref|NP_079491.1|
pseudouridylate synthase 1; pseudouridine synthase 1
[Homo sapiens] gi|23503104|sp|Q9Y606|TRUA_HUMAN tRNA
pseudouridine synthase A (tRNA-uridine isomerase I)
(tRNA pseudouridylate synthase I)
Length = 399
Score = 99.0 bits (245), Expect = 3e-19
Identities = 62/206 (30%), Positives = 105/206 (50%), Gaps = 23/206 (11%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ ++G I E++ D+ K+ + R
Sbjct: 55 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 114
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + +NS+LP I+++ + F+
Sbjct: 115 ARTDKGVSAAGQVVSLKVWLIDD----------ILEKINSHLPSHIRILGLKRVTGGFNS 164
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ C R Y YLLP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 165 KNRCDARTYCYLLPTFAFAHKDRDVQDE-TYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 223
Query: 231 SRYRRHIPRRPSHSKCGEILSAYNSE 256
P+ P S C IL Y E
Sbjct: 224 KG-----PQDP--SACRYILEMYCEE 242
Score = 41.6 bits (96), Expect = 0.059
Identities = 22/61 (36%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S ++ +P AP L+
Sbjct: 248 GLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT-EKVDVPKAPGLGLV 306
Query: 406 L 406
L
Sbjct: 307 L 307
>gb|AAL55876.1| unknown [Homo sapiens]
Length = 427
Score = 99.0 bits (245), Expect = 3e-19
Identities = 62/206 (30%), Positives = 105/206 (50%), Gaps = 23/206 (11%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ ++G I E++ D+ K+ + R
Sbjct: 83 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 142
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + +NS+LP I+++ + F+
Sbjct: 143 ARTDKGVSAAGQVVSLKVWLIDD----------ILEKINSHLPSHIRILGLKRVTGGFNS 192
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ C R Y YLLP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 193 KNRCDARTYCYLLPTFAFAHKDRDVQDE-TYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 251
Query: 231 SRYRRHIPRRPSHSKCGEILSAYNSE 256
P+ P S C IL Y E
Sbjct: 252 KG-----PQDP--SACRYILEMYCEE 270
Score = 41.6 bits (96), Expect = 0.059
Identities = 22/61 (36%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S ++ +P AP L+
Sbjct: 276 GLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT-EKVDVPKAPGLGLV 334
Query: 406 L 406
L
Sbjct: 335 L 335
>gb|AAD21042.1| pseudouridine synthase 1 [Homo sapiens]
Length = 365
Score = 99.0 bits (245), Expect = 3e-19
Identities = 62/206 (30%), Positives = 105/206 (50%), Gaps = 23/206 (11%)
Query: 56 KKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWARS 115
K+K+V+ +AY+G Y G+Q + T+E +L SA+ ++G I E++ D+ K+ + R
Sbjct: 21 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 80
Query: 116 SRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDP 175
+RTDKGV + +V+ K+ + ++ + +NS+LP I+++ + F+
Sbjct: 81 ARTDKGVSAAGQVVSLKVWLIDD----------ILEKINSHLPSHIRILGLKRVTGGFNS 130
Query: 176 RKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMS-----EFNDILKEFEGGHPFHNYTSR 230
+ C R Y YLLP + DE Y +S + N +L ++G H FHN+TS+
Sbjct: 131 KNRCDARTYCYLLPTFAFAHKDRDVQDE-TYRLSAETLQQVNRLLACYKGTHNFHNFTSQ 189
Query: 231 SRYRRHIPRRPSHSKCGEILSAYNSE 256
P+ P S C IL Y E
Sbjct: 190 KG-----PQDP--SACRYILEMYCEE 208
Score = 41.6 bits (96), Expect = 0.059
Identities = 22/61 (36%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
Query: 346 GHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLL 405
G + I ++G+SFM+HQIRKMV VA+ + P ++ S ++ +P AP L+
Sbjct: 214 GLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT-EKVDVPKAPGLGLV 272
Query: 406 L 406
L
Sbjct: 273 L 273
>gb|AAH86827.1| Zgc:103516 [Danio rerio] gi|56693277|ref|NP_001008603.1|
hypothetical protein LOC494060 [Danio rerio]
Length = 376
Score = 98.2 bits (243), Expect = 5e-19
Identities = 59/200 (29%), Positives = 101/200 (50%), Gaps = 15/200 (7%)
Query: 54 FRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWA 113
+ KKKV + +AY+G Y G+Q + T+E EL +A+ A I E + +++K+ +
Sbjct: 37 YPKKKVALLMAYSGKGYYGMQRNARNSQFKTIEDELVTALVGARCIPEGHAEEMKKMSFQ 96
Query: 114 RSSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSF 173
RS+RTDKGV ++ +V+ K+ + EN + +N++LP I+V+ F
Sbjct: 97 RSARTDKGVSAVGRVVSLKVWMIEN----------ILEKINAHLPPRIRVLGCKRVTGGF 146
Query: 174 DPRKECVLRKYSYLLPADIIGIQSHSSNDE----IDYHMSEFNDILKEFEGGHPFHNYTS 229
+ + C R YSY+LP + ++ D + N + ++G H FHN+TS
Sbjct: 147 NSKNNCYARTYSYMLPTVAFSPKDYNQEDTSFRLTSDTLQTVNRLFCLYKGTHNFHNFTS 206
Query: 230 RSRYR-RHIPRRPSHSKCGE 248
+ +R R +H CGE
Sbjct: 207 QKGHRDPSAMRYITHMSCGE 226
Score = 35.0 bits (79), Expect = 5.5
Identities = 28/113 (24%), Positives = 52/113 (45%), Gaps = 5/113 (4%)
Query: 352 IYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLLRGNSF 411
I + G+SFM+HQIRKM+ +AV + + ++ S + ++ + AP L+L F
Sbjct: 238 ITVRGQSFMMHQIRKMIGLVIAVVKGYVDEGVMEKSWGE-EKVGVSKAPGLGLVLERVHF 296
Query: 412 SMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQV--SKFLDPSRVPWV 462
K + +E E + + DF + P + ++ + S V W+
Sbjct: 297 DGYNK--RFGGDGIHETLEWTEQEEAIADFKEKHIYPTIVDTELNERSMVNWM 347
>ref|XP_656284.1| tRNA pseudouridine synthase, putative [Entamoeba histolytica
HM-1:IMSS] gi|56473473|gb|EAL50898.1| tRNA pseudouridine
synthase, putative [Entamoeba histolytica HM-1:IMSS]
Length = 401
Score = 94.4 bits (233), Expect = 8e-18
Identities = 56/188 (29%), Positives = 94/188 (49%), Gaps = 26/188 (13%)
Query: 55 RKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKIGWAR 114
+K KV + +Y G Y GL N+ T+E E+E+A++KA I E N DL+K+GW+R
Sbjct: 39 KKFKVAVIFSYVGRGYFGLMYLN--NNPKTIEGEIENAMYKAKCISEDNKHDLKKVGWSR 96
Query: 115 SSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFD 174
+RTDKGV + +++ KM + N +NS+LP I++ ++ SF+
Sbjct: 97 CARTDKGVSAANNLISLKMGLDGN----------YIERINSFLPETIRIQDVVKVSHSFN 146
Query: 175 PRKECVLRKYSYLLPA----------DIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPF 224
+ R Y Y+ P+ I ++ + ++IDY FN ++ + H F
Sbjct: 147 AKTRVDSRTYEYIFPSYAIQKIDSEHPEINVEYNPQQEDIDY----FNSVISNYVKTHNF 202
Query: 225 HNYTSRSR 232
NYT ++
Sbjct: 203 QNYTFNTK 210
Score = 41.6 bits (96), Expect = 0.059
Identities = 37/143 (25%), Positives = 68/143 (46%), Gaps = 7/143 (4%)
Query: 338 SGKLEKSMGHSYIEIYIEGESFMLHQIRKMVATAVAVKRKLLP----RDIITLSLSKFS- 392
S +L + G + I + I G+SFML QIR+M+ A+ + LP +I+ S + +
Sbjct: 227 SAQLVQRNGLNVIAVSIHGQSFMLWQIRRMIGFAIVLTNFRLPIEQMNEIMKTSFTLINW 286
Query: 393 RIILPLAPSEVLLLRGNSF-SMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQVS 451
R ++P AP+ L L F + + T +T + SEE K +F + +
Sbjct: 287 RSVIPTAPAHGLFLDQCHFGNHNERLQRDTSNNSKTNINSEETLKKCMEFKEKYIWNDII 346
Query: 452 KFLDPSRVPWVEWIEKFDAHTSI 474
F + P+ +++++ +T I
Sbjct: 347 NF-EKEEQPFAKYMKEQVNYTKI 368
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.317 0.132 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 870,614,455
Number of Sequences: 2540612
Number of extensions: 37170360
Number of successful extensions: 94165
Number of sequences better than 10.0: 386
Number of HSP's better than 10.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 304
Number of HSP's that attempted gapping in prelim test: 93504
Number of HSP's gapped (non-prelim): 582
length of query: 501
length of database: 863,360,394
effective HSP length: 132
effective length of query: 369
effective length of database: 527,999,610
effective search space: 194831856090
effective search space used: 194831856090
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 77 (34.3 bits)
Medicago: description of AC140068.2