BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138017.12 + phase: 1 /pseudo
(95 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB80446.1| hypothetical protein [Arabidopsis thaliana] gi|4... 87 1e-16
ref|XP_476522.1| unknown protein [Oryza sativa (japonica cultiva... 85 4e-16
ref|XP_469232.1| hypothetical protein [Oryza sativa (japonica cu... 80 1e-14
gb|AAS07151.1| hypothetical protein [Oryza sativa (japonica cult... 72 4e-12
gb|AAR24177.1| At5g10310 [Arabidopsis thaliana] gi|8953412|emb|C... 68 5e-11
ref|XP_467871.1| unknown protein [Oryza sativa (japonica cultiva... 59 3e-08
dbj|BAB01257.1| unnamed protein product [Arabidopsis thaliana] g... 45 3e-04
ref|NP_850143.1| allergen-related [Arabidopsis thaliana] 44 8e-04
gb|AAN06864.1| Hypothetical protein [Oryza sativa (japonica cult... 42 0.003
dbj|BAD87082.1| allergen-like protein [Oryza sativa (japonica cu... 41 0.006
gb|AAT77087.1| hypothetical protein [Oryza sativa (japonica cult... 39 0.032
ref|XP_483039.1| type 1a plasma membrane receptor-like [Oryza sa... 39 0.042
gb|AAD02823.1| putative type 1a plasma membrane receptor [Oryza ... 39 0.042
ref|YP_122039.1| hypothetical protein pnf11500 [Nocardia farcini... 37 0.094
sp|Q7M1E7|PGLR2_CHAOB Polygalacturonase precursor (PG) (Pectinas... 37 0.094
emb|CAC05582.1| pollen major allergen 2 protein [Juniperus ashei... 33 1.8
dbj|BAC23082.1| allergen Cry j 2 [Cryptomeria japonica] 33 1.8
dbj|BAA06172.1| allergen [Cryptomeria japonica] 32 3.0
dbj|BAC23084.1| allergen Cry j 2 [Cryptomeria japonica] 32 3.0
dbj|BAC23083.1| allergen Cry j 2 [Cryptomeria japonica] 32 3.0
>emb|CAB80446.1| hypothetical protein [Arabidopsis thaliana]
gi|4490726|emb|CAB38929.1| hypothetical protein
[Arabidopsis thaliana] gi|22329225|ref|NP_680774.1|
expressed protein [Arabidopsis thaliana]
gi|7487503|pir||T06028 hypothetical protein T28I19.90 -
Arabidopsis thaliana
Length = 128
Score = 87.0 bits (214), Expect = 1e-16
Identities = 49/103 (47%), Positives = 66/103 (63%), Gaps = 12/103 (11%)
Query: 2 GRPISNLNEVAKNGMEENGSMLAEKLQIGSRPPKCER-RCRSCVHCEAVQVPIVPSKVQI 60
GRP + E K+G +++ M+ L IGSRPP+CER RCRSC HCEA+QVP P + ++
Sbjct: 28 GRPEPDSVEFTKSG-DQDVKMMMRGL-IGSRPPRCERVRCRSCGHCEAIQVPTNP-QTKL 84
Query: 61 HRSHYDSAAYSS--------RGDGLSNYKPISWKCKCGDYFFN 95
H S++ SS RGD +NYKP+SWKCKCG+ +N
Sbjct: 85 HSPLTTSSSSSSETIHLDYTRGDDSTNYKPMSWKCKCGNSIYN 127
>ref|XP_476522.1| unknown protein [Oryza sativa (japonica cultivar-group)]
gi|34393745|dbj|BAC83285.1| unknown protein [Oryza
sativa (japonica cultivar-group)]
Length = 155
Score = 85.1 bits (209), Expect = 4e-16
Identities = 45/104 (43%), Positives = 57/104 (54%), Gaps = 27/104 (25%)
Query: 14 NGMEENGSMLAEKLQ--------IGSRPPKCERRCRSCVHCEAVQVPIVPSKVQ------ 59
N EE + EK++ IGSRPP+CER C SC HCEAVQVPIVP +Q
Sbjct: 46 NIKEEPDETIGEKMEMEMEGRRLIGSRPPRCERVCMSCGHCEAVQVPIVPQVIQKTQTKA 105
Query: 60 -------------IHRSHYDSAAYSSRGDGLSNYKPISWKCKCG 90
+ + +A ++ R +GLSNYKP+SWKCKCG
Sbjct: 106 AAAAAAEQEQHVVVSATAISAAVFTYRVNGLSNYKPLSWKCKCG 149
>ref|XP_469232.1| hypothetical protein [Oryza sativa (japonica cultivar-group)]
gi|29788841|gb|AAP03387.1| hypothetical protein [Oryza
sativa (japonica cultivar-group)]
Length = 127
Score = 80.5 bits (197), Expect = 1e-14
Identities = 45/104 (43%), Positives = 52/104 (49%), Gaps = 20/104 (19%)
Query: 12 AKNGMEENGSMLAEKLQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYD----- 66
A G E + + IGSRPP CE RCRSC HCEAVQVPI P ++Q R D
Sbjct: 23 AAAGFTEEKGVAGIRGVIGSRPPSCEGRCRSCGHCEAVQVPITPQQLQKKRGQGDDRRRK 82
Query: 67 ----------SAAYSSRG-----DGLSNYKPISWKCKCGDYFFN 95
+AA R D SNYKP+SW+CKCG N
Sbjct: 83 KKQLLGHGDRAAAAGGRAMPDSYDDHSNYKPLSWRCKCGGMILN 126
>gb|AAS07151.1| hypothetical protein [Oryza sativa (japonica cultivar-group)]
Length = 135
Score = 71.6 bits (174), Expect = 4e-12
Identities = 31/64 (48%), Positives = 41/64 (63%), Gaps = 1/64 (1%)
Query: 29 IGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLS-NYKPISWKC 87
IGSRPP+CE +C C CEAVQVP+ P ++ + AA + D S NYKP++WKC
Sbjct: 65 IGSRPPRCEGKCAPCGRCEAVQVPVAPRAGRLRAFFFSRAAAADDDDESSTNYKPLNWKC 124
Query: 88 KCGD 91
+C D
Sbjct: 125 RCAD 128
>gb|AAR24177.1| At5g10310 [Arabidopsis thaliana] gi|8953412|emb|CAB96687.1|
putative protein [Arabidopsis thaliana]
gi|15238119|ref|NP_196593.1| expressed protein
[Arabidopsis thaliana] gi|40823959|gb|AAR92318.1|
At5g10310 [Arabidopsis thaliana] gi|11285694|pir||T50819
hypothetical protein F18D22_80 - Arabidopsis thaliana
Length = 122
Score = 68.2 bits (165), Expect = 5e-11
Identities = 29/82 (35%), Positives = 48/82 (58%), Gaps = 7/82 (8%)
Query: 21 SMLAEKLQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAY-------SSR 73
+++ +K ++GS PP C RC +C C A+QVP +P++ + R + S + ++
Sbjct: 40 ALIEDKARLGSTPPSCHNRCNNCHPCMAIQVPTLPTRSRFTRVNPFSGGFVRPPSSLTTV 99
Query: 74 GDGLSNYKPISWKCKCGDYFFN 95
D SNYKP+ WKC C +F+N
Sbjct: 100 LDQYSNYKPMGWKCHCNGHFYN 121
>ref|XP_467871.1| unknown protein [Oryza sativa (japonica cultivar-group)]
gi|46805961|dbj|BAD17255.1| unknown protein [Oryza
sativa (japonica cultivar-group)]
Length = 110
Score = 58.9 bits (141), Expect = 3e-08
Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 6/82 (7%)
Query: 16 MEENGSMLAEKLQIGSRPPKCERRCRSCVHCEAVQVPIVP--SKVQIHRSHYDSAAYSSR 73
+ + S+ +K ++GS PP C RC +C C VQV +P S+ D A ++
Sbjct: 32 LSSSQSLAEDKSRLGSTPPSCHNRCNACNPCTPVQVAALPGVSRPASAADRVDVAGFAQ- 90
Query: 74 GDGLSNYKPISWKCKCGDYFFN 95
SNYKP+ WKC+C F+
Sbjct: 91 ---YSNYKPLGWKCRCAGRLFD 109
>dbj|BAB01257.1| unnamed protein product [Arabidopsis thaliana]
gi|48310314|gb|AAT41796.1| At3g22820 [Arabidopsis
thaliana] gi|46931210|gb|AAT06409.1| At3g22820
[Arabidopsis thaliana] gi|15228864|ref|NP_188921.1|
allergen-related [Arabidopsis thaliana]
Length = 107
Score = 45.4 bits (106), Expect = 3e-04
Identities = 27/87 (31%), Positives = 40/87 (45%), Gaps = 21/87 (24%)
Query: 10 EVAKNGMEENGSMLAEKLQ--IGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDS 67
E+A++G+ G ++ +K GS PP C +C C C+AV VPI P +
Sbjct: 38 EIARSGLP--GQIVDQKRLGGPGSVPPMCRLKCGKCEPCKAVHVPIQPGLIM-------- 87
Query: 68 AAYSSRGDGLSNYKPISWKCKCGDYFF 94
Y P +W+CKCG+ F
Sbjct: 88 ---------PLEYYPEAWRCKCGNKLF 105
>ref|NP_850143.1| allergen-related [Arabidopsis thaliana]
Length = 230
Score = 44.3 bits (103), Expect = 8e-04
Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 17/66 (25%)
Query: 29 IGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISWKCK 88
+GS PP+C +C C C+ V VP+ P + Y P +W+CK
Sbjct: 180 LGSSPPRCSSKCGRCTPCKPVHVPVPPGT-----------------PVTAEYYPEAWRCK 222
Query: 89 CGDYFF 94
CG+ +
Sbjct: 223 CGNKLY 228
>gb|AAN06864.1| Hypothetical protein [Oryza sativa (japonica cultivar-group)]
Length = 151
Score = 42.4 bits (98), Expect = 0.003
Identities = 22/65 (33%), Positives = 25/65 (37%), Gaps = 17/65 (26%)
Query: 30 GSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISWKCKC 89
GS PP C RC C C V V I P + Y P +W+CKC
Sbjct: 102 GSSPPTCRSRCGGCHPCRPVHVAIQPGR-----------------SFPLEYYPEAWRCKC 144
Query: 90 GDYFF 94
GD F
Sbjct: 145 GDKLF 149
>dbj|BAD87082.1| allergen-like protein [Oryza sativa (japonica cultivar-group)]
Length = 139
Score = 41.2 bits (95), Expect = 0.006
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 17/65 (26%)
Query: 30 GSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISWKCKC 89
GS PP+C +C +CV C V V VP V + +Y P +W+CKC
Sbjct: 90 GSYPPRCTAKCGACVPCYPVHV-AVPPGVPVTTEYY----------------PEAWRCKC 132
Query: 90 GDYFF 94
G+ +
Sbjct: 133 GNRLY 137
>gb|AAT77087.1| hypothetical protein [Oryza sativa (japonica cultivar-group)]
Length = 152
Score = 38.9 bits (89), Expect = 0.032
Identities = 15/22 (68%), Positives = 17/22 (77%)
Query: 29 IGSRPPKCERRCRSCVHCEAVQ 50
IGSRPP+CE +C C CEAVQ
Sbjct: 57 IGSRPPRCEGKCAPCGRCEAVQ 78
>ref|XP_483039.1| type 1a plasma membrane receptor-like [Oryza sativa (japonica
cultivar-group)] gi|42409410|dbj|BAD10723.1| type 1a
plasma membrane receptor-like [Oryza sativa (japonica
cultivar-group)] gi|42408172|dbj|BAD09309.1| type 1a
plasma membrane receptor-like [Oryza sativa (japonica
cultivar-group)]
Length = 116
Score = 38.5 bits (88), Expect = 0.042
Identities = 15/28 (53%), Positives = 19/28 (67%)
Query: 25 EKLQIGSRPPKCERRCRSCVHCEAVQVP 52
EK+++GS PP C +C C C AVQVP
Sbjct: 60 EKVRLGSSPPSCYSKCYGCSPCVAVQVP 87
>gb|AAD02823.1| putative type 1a plasma membrane receptor [Oryza sativa]
Length = 196
Score = 38.5 bits (88), Expect = 0.042
Identities = 15/28 (53%), Positives = 19/28 (67%)
Query: 25 EKLQIGSRPPKCERRCRSCVHCEAVQVP 52
EK+++GS PP C +C C C AVQVP
Sbjct: 60 EKVRLGSSPPSCYSKCYGCSPCVAVQVP 87
>ref|YP_122039.1| hypothetical protein pnf11500 [Nocardia farcinica IFM 10152]
gi|54019306|dbj|BAD60675.1| hypothetical protein
[Nocardia farcinica IFM 10152]
Length = 356
Score = 37.4 bits (85), Expect = 0.094
Identities = 19/73 (26%), Positives = 37/73 (50%), Gaps = 1/73 (1%)
Query: 14 NGMEENGSMLAEKLQIGSRPPKCERRC-RSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSS 72
+G ++N S KL G+ PP+ E R+ CE + P++ ++++ + +A +
Sbjct: 30 DGDKDNPSFAQGKLATGTVPPEYEAWVIRAGTMCEEISAPLIAAQIEQESNWNPNAVSPA 89
Query: 73 RGDGLSNYKPISW 85
GLS + P +W
Sbjct: 90 GAQGLSQFMPYTW 102
>sp|Q7M1E7|PGLR2_CHAOB Polygalacturonase precursor (PG) (Pectinase) (Major pollen allergen
Cha o 2)
Length = 514
Score = 37.4 bits (85), Expect = 0.094
Identities = 20/84 (23%), Positives = 34/84 (39%), Gaps = 23/84 (27%)
Query: 16 MEENGSMLAEK----LQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYS 71
M+EN A+ + + S PP C+ +C+ C C+ + + P+K Q
Sbjct: 449 MDENKGACAKGDSTCISLSSSPPNCKNKCKGCQPCKPKLIIVHPNKPQ------------ 496
Query: 72 SRGDGLSNYKPISWKCKCGDYFFN 95
+Y P W C C + +N
Sbjct: 497 -------DYYPQKWVCSCHNKIYN 513
>emb|CAC05582.1| pollen major allergen 2 protein [Juniperus ashei]
gi|47606048|sp|Q9FY19|PGLR2_JUNAS Polygalacturonase
precursor (PG) (Pectinase) (Major pollen allergen Jun a
2)
Length = 507
Score = 33.1 bits (74), Expect = 1.8
Identities = 16/76 (21%), Positives = 32/76 (42%), Gaps = 21/76 (27%)
Query: 22 MLAEKL--QIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSN 79
++ EK+ + S PP C+ +C+ C C+ + + P++ + +
Sbjct: 450 LMDEKMGASLNSSPPNCKNKCKGCQPCKPKLIIVHPNQPE-------------------D 490
Query: 80 YKPISWKCKCGDYFFN 95
Y P W C C + +N
Sbjct: 491 YYPQRWVCSCHNKIYN 506
>dbj|BAC23082.1| allergen Cry j 2 [Cryptomeria japonica]
Length = 514
Score = 33.1 bits (74), Expect = 1.8
Identities = 18/64 (28%), Positives = 24/64 (37%), Gaps = 19/64 (29%)
Query: 26 KLQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISW 85
++ +GSRPP C +C C C+A V +HR Y P W
Sbjct: 463 RILLGSRPPNCTNKCHGCSPCKA-------KLVIVHRIM------------PQEYYPQRW 503
Query: 86 KCKC 89
C C
Sbjct: 504 MCSC 507
>dbj|BAA06172.1| allergen [Cryptomeria japonica]
Length = 514
Score = 32.3 bits (72), Expect = 3.0
Identities = 18/64 (28%), Positives = 24/64 (37%), Gaps = 19/64 (29%)
Query: 26 KLQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISW 85
++ +GSRPP C +C C C+A V +HR Y P W
Sbjct: 463 RILLGSRPPNCTNKCHGCSPCKA-------KLVIVHRIM------------PQEYYPQRW 503
Query: 86 KCKC 89
C C
Sbjct: 504 ICSC 507
>dbj|BAC23084.1| allergen Cry j 2 [Cryptomeria japonica]
Length = 514
Score = 32.3 bits (72), Expect = 3.0
Identities = 18/64 (28%), Positives = 24/64 (37%), Gaps = 19/64 (29%)
Query: 26 KLQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISW 85
++ +GSRPP C +C C C+A V +HR Y P W
Sbjct: 463 RILLGSRPPNCTNKCHGCSPCKA-------KLVIVHRIM------------PQEYYPQRW 503
Query: 86 KCKC 89
C C
Sbjct: 504 ICSC 507
>dbj|BAC23083.1| allergen Cry j 2 [Cryptomeria japonica]
Length = 514
Score = 32.3 bits (72), Expect = 3.0
Identities = 18/64 (28%), Positives = 24/64 (37%), Gaps = 19/64 (29%)
Query: 26 KLQIGSRPPKCERRCRSCVHCEAVQVPIVPSKVQIHRSHYDSAAYSSRGDGLSNYKPISW 85
++ +GSRPP C +C C C+A V +HR Y P W
Sbjct: 463 RILLGSRPPNCTNKCHGCSPCKA-------KLVIVHRIM------------PQEYYPQRW 503
Query: 86 KCKC 89
C C
Sbjct: 504 ICSC 507
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.318 0.134 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 179,044,112
Number of Sequences: 2540612
Number of extensions: 6268667
Number of successful extensions: 11801
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 11769
Number of HSP's gapped (non-prelim): 21
length of query: 95
length of database: 863,360,394
effective HSP length: 71
effective length of query: 24
effective length of database: 682,976,942
effective search space: 16391446608
effective search space used: 16391446608
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC138017.12