BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134822.9 + phase: 0
(609 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK93754.1| putative pectinesterase [Arabidopsis thaliana] gi... 766 0.0
gb|AAP37714.1| At3g49220 [Arabidopsis thaliana] gi|6723392|emb|C... 754 0.0
ref|NP_913537.1| unnamed protein product [Oryza sativa (japonica... 561 e-158
ref|XP_465003.1| putative pectinesterase 2 precursor [Oryza sati... 505 e-141
gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis thaliana] gi|92795... 479 e-133
emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum] 479 e-133
gb|AAM91439.1| At1g53830/T18A20_6 [Arabidopsis thaliana] gi|6056... 476 e-133
gb|AAF23892.1| pectin methyl esterase [Solanum tuberosum] 476 e-132
gb|AAC72288.1| putative pectin methylesterase [Arabidopsis thali... 475 e-132
gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana] 473 e-132
sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methyles... 472 e-131
gb|AAC50023.1| ATPME2 precursor [Arabidopsis thaliana] 469 e-131
gb|AAB57670.1| pectinesterase [Citrus sinensis] 469 e-130
sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methyles... 469 e-130
emb|CAE76634.1| pectin methylesterase [Cicer arietinum] 464 e-129
gb|AAL02367.1| pectin methylesterase [Lycopersicon esculentum] g... 463 e-129
gb|AAK84486.1| putative thermostable pectinesterase [Citrus sine... 462 e-128
emb|CAE76633.2| pectin methylesterase [Cicer arietinum] 457 e-127
dbj|BAD45460.1| putative pectinesterase [Oryza sativa (japonica ... 450 e-125
dbj|BAC67661.1| pectin methylesterase [Pisum sativum] 449 e-125
>gb|AAK93754.1| putative pectinesterase [Arabidopsis thaliana]
gi|13507549|gb|AAK28637.1| putative pectinesterase
[Arabidopsis thaliana] gi|9759184|dbj|BAB09799.1|
pectinesterase [Arabidopsis thaliana]
gi|15238729|ref|NP_200149.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 587
Score = 766 bits (1977), Expect = 0.0
Identities = 385/611 (63%), Positives = 467/611 (76%), Gaps = 28/611 (4%)
Query: 1 MDFDRLGPSDPGGSSRRSTAPLTPSTTSNSSPKSNKKLIMLSILAAVLIIASAISAALIT 60
M +DRLGPS P +++ A P K+ KLI+ ++ AVL++ + +
Sbjct: 1 MGYDRLGPSGPSNPNQKDPATSLPELQK----KTKTKLILFTL--AVLVVG-VVCFGIFA 53
Query: 61 VVRSRASSNNSNLLHSKPTQAISRTCSKTRYPSLCINSLLDFPGSTSASEQELVHISFNM 120
+R+ S L KPTQAISRTCSK+ YP+LCI++LLDFPGS +A E EL+HISFN
Sbjct: 54 GIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNA 113
Query: 121 THRHISKALFASSGLSYTVANPRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDD 180
T + SKAL+ SS ++YT PRVR+AY+ CLEL+D+S+DA+ ++ S++ +S D+
Sbjct: 114 TLQKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVV----VSGDE 169
Query: 181 GESRQFSNVAGSTEDVMTWLSAALTNQDTCLEGFEDTSGT---VKDQMVGNLKDLSELVS 237
S DVMTWLS+A+TN DTC +GF++ G VKDQ++G +KDLSE+VS
Sbjct: 170 SHS-----------DVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVS 218
Query: 238 NSLAIFSASGDNDFTGVPIQNKRRLMGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIV 297
N LAIF A D +GVP+ N R+L+G + + E P WL++ DR LL P S IQADI V
Sbjct: 219 NCLAIF-AGKVKDLSGVPVVNNRKLLGTEE-TEELPNWLKREDRELLGTPTSAIQADITV 276
Query: 298 SKSGGNGTVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKT 357
SK G +GT KTI EAIKKAPEHS RRF+IYV+AGRYEE NLKVG+KKTN+MFIGDG+GKT
Sbjct: 277 SKDG-SGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKT 335
Query: 358 VITGKRSVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRC 417
VITG +S+ D +TTFHTA+FAA+G GF+ RD+TFENYAGP KHQAVALRVG DHAVVYRC
Sbjct: 336 VITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRC 395
Query: 418 NIVGYQDACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQN 477
NI+GYQDA YVHSNRQFFREC IYGTVDFIFGNAAV+ Q CNIYARKPMAQQK TITAQN
Sbjct: 396 NIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQN 455
Query: 478 RKDPNQNTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGW 537
RKDPNQNTGISIH C++L PDL +SKGS TYLGRPWK+YSR VYM+S MGDH+ P GW
Sbjct: 456 RKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGW 515
Query: 538 LEWNGDFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLP 597
LEWNG FAL +LYYGEYMN G G+ IGQRVKWPGY VITST+EA+++TVAQFISGSSWLP
Sbjct: 516 LEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLP 575
Query: 598 STGVAFLAGLS 608
STGV+F +GLS
Sbjct: 576 STGVSFFSGLS 586
>gb|AAP37714.1| At3g49220 [Arabidopsis thaliana] gi|6723392|emb|CAB66401.1|
pectinesterase-like protein [Arabidopsis thaliana]
gi|16648929|gb|AAL24316.1| pectinesterase-like protein
[Arabidopsis thaliana] gi|15229105|ref|NP_190491.1|
pectinesterase family protein [Arabidopsis thaliana]
gi|11279196|pir||T45827 pectinesterase-like protein -
Arabidopsis thaliana
Length = 598
Score = 754 bits (1948), Expect = 0.0
Identities = 382/619 (61%), Positives = 479/619 (76%), Gaps = 33/619 (5%)
Query: 1 MDFDRLGPSDPGGSSRRST--APLTPSTTSNSSPKSN-----KKLIMLSILAAVLIIASA 53
M ++RLGPS GS ST AP+ +++ P++N KKL++ SI+ A+ +I
Sbjct: 1 MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLI--- 57
Query: 54 ISAALITVVRSRASSNNSNL-LHSKPTQAISRTCSKTRYPSLCINSLLDFPGSTSASE-Q 111
++AA+ VRSR N S L KP+QAIS+ C TR+P LC++SL+DFPGS +AS +
Sbjct: 58 LAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSK 117
Query: 112 ELVHISFNMTHRHISKALFASSGLSYTVANPRVRAAYEDCLELMDESMDAIRSSMDSLMT 171
+L+H++ NMT H S AL++S+ LS+ PR R+AY+ C+EL+D+S+DA+ ++ S+++
Sbjct: 118 DLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVS 177
Query: 172 TSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQDTCLEGFEDTS-GTVKDQMVGNLK 230
+S+ +DV TWLSAALTN DTC EGF+ G VKD M L+
Sbjct: 178 SSA-----------------KPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQ 220
Query: 231 DLSELVSNSLAIFSASGD-NDFTGVPIQNKRRLMGMSDISREFPKWLEKRDRRLLSLPVS 289
+LSELVSN LAIFSAS D +DF GVPIQN RRL+G+ + +FP+W+ ++R +L +PVS
Sbjct: 221 NLSELVSNCLAIFSASHDGDDFAGVPIQN-RRLLGVEEREEKFPRWMRPKEREILEMPVS 279
Query: 290 EIQADIIVSKSGGNGTVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMF 349
+IQADIIVSK G NGT KTI+EAIKKAP++S RR IIYV+AGRYEENNLKVG+KK N+MF
Sbjct: 280 QIQADIIVSKDG-NGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMF 338
Query: 350 IGDGRGKTVITGKRSVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGS 409
+GDG+GKTVI+G +S+ D +TTFHTASFAA+G GF+ARDITFEN+AGP KHQAVALR+G+
Sbjct: 339 VGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGA 398
Query: 410 DHAVVYRCNIVGYQDACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQ 469
DHAV+YRCNI+GYQD YVHSNRQFFREC+IYGTVDFIFGNAAVV Q C+IYARKPM Q
Sbjct: 399 DHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQ 458
Query: 470 KNTITAQNRKDPNQNTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMG 529
KNTITAQNRKDPNQNTGISIH R+L A DL ++ GS +TYLGRPWK++SRTVYM+SY+G
Sbjct: 459 KNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIG 518
Query: 530 DHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQF 589
HVH GWLEWN FAL TLYYGEY+N GPG+ +GQRV WPGYRVI ST EANR+TVA+F
Sbjct: 519 GHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEF 578
Query: 590 ISGSSWLPSTGVAFLAGLS 608
I GSSWLPSTGV+FLAGLS
Sbjct: 579 IYGSSWLPSTGVSFLAGLS 597
>ref|NP_913537.1| unnamed protein product [Oryza sativa (japonica cultivar-group)]
Length = 621
Score = 561 bits (1445), Expect = e-158
Identities = 290/599 (48%), Positives = 397/599 (65%), Gaps = 35/599 (5%)
Query: 38 LIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSK---PTQAISRTCSKTRYPSL 94
+++L I+ L + A++ A+ + RSR +S++ + PT+AI+RTC T YP L
Sbjct: 29 IVVLCIVGVALAVGVAVAVAIAVLGRSRMTSSSGGGRAPRGRAPTEAIARTCGVTLYPEL 88
Query: 95 CINSLLDFPGSTSASEQELVHISFNMTHRHISKALF---ASSGLSYTVANPRVRAAYEDC 151
C+ L+ FPG+ A + ELV +S N THR + AL+ A G + +A R AAY DC
Sbjct: 89 CVGELMAFPGAAGAGDAELVPMSLNATHRRVVDALYNATALGGAAALLAGARSGAAYGDC 148
Query: 152 LELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQDTCL 211
+E++D + + + S+ ++ + D ++ +D+MTWLSAALT+ DTC+
Sbjct: 149 VEMLDAAEELLARSVGAIAAPPPPPDSVDADT-----AGRDDDDIMTWLSAALTSHDTCM 203
Query: 212 EGFE----------DTSGTVKDQMVGNLKDLSELVSNSLAIFSASG--DNDFTGVPIQNK 259
+ + D G +K QM+G L +L E +SNSLAIF+A G + + VP+ N+
Sbjct: 204 DSLQEVGAGGDAGDDDGGRIKPQMLGYLGNLGEHLSNSLAIFAARGRPGGELSDVPVHNQ 263
Query: 260 --RRLMGMSDISRE---FPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIK 314
RRL+ + D + FP+W+ DRRLL +EI+AD++V+K G GT + I +AIK
Sbjct: 264 LHRRLLTIDDDDDDDGSFPRWVRHNDRRLLQAAAAEIEADMVVAKDG-TGTHRKIRDAIK 322
Query: 315 KAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHT 374
APEHSRRR +IYV+AG Y EN +K+G KKTN+M +GDG GKTV+ G RSV D TTFHT
Sbjct: 323 AAPEHSRRRVVIYVKAGVYTEN-VKIGSKKTNLMLVGDGAGKTVVVGYRSVHDNYTTFHT 381
Query: 375 ASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQF 434
A+ A +G GF+ RD+T EN AG +HQAVAL + DHAVVYR ++GYQD Y H+ RQF
Sbjct: 382 ATLAVAGAGFIMRDMTVENRAGAARHQAVALLLSGDHAVVYRSAVLGYQDTLYAHAQRQF 441
Query: 435 FRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRI 494
+R+C++ GTVDF+FGNAAVV Q C ++AR+P+ Q+NT+TAQ R+DPNQ+TGIS+H CR+
Sbjct: 442 YRDCDVAGTVDFVFGNAAVVLQNCTLWARRPLPGQENTVTAQGRRDPNQSTGISVHGCRL 501
Query: 495 LPAPDL----ASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGD-FALKTL 549
LP+P+L A+ +G TYLGRPWK YSR VYM+SY+ HVH GWL W+ A TL
Sbjct: 502 LPSPELELAPAARRGRAATYLGRPWKPYSRAVYMMSYIAGHVHAAGWLAWDASGRAPDTL 561
Query: 550 YYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGLS 608
YYGEY N GPGAA+G RV WPG+RVI EA +TV +FI G SWLP TGVAF+AGL+
Sbjct: 562 YYGEYRNSGPGAAVGGRVPWPGHRVIKLPEEAMEFTVGRFIGGYSWLPPTGVAFVAGLT 620
>ref|XP_465003.1| putative pectinesterase 2 precursor [Oryza sativa (japonica
cultivar-group)] gi|47847929|dbj|BAD21719.1| putative
pectinesterase 2 precursor [Oryza sativa (japonica
cultivar-group)]
Length = 554
Score = 505 bits (1300), Expect = e-141
Identities = 259/537 (48%), Positives = 351/537 (65%), Gaps = 59/537 (10%)
Query: 108 ASEQELVHISFNMTHRHISKALFASSGLSYTVAN---------------PRVRAAYEDCL 152
A+ + I+ N T H+ KAL A SG + A+ P AA +DC
Sbjct: 40 AARHHRLRIALNATAVHVGKALEALSGAAVAPASAVMTTPFSAGSSSSSPLAAAARDDCA 99
Query: 153 ELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQDTCLE 212
EL+++S+D + + GE + + +D +TWLSAALTN DTC +
Sbjct: 100 ELLEDSLDLLAGA---------------GEP------SAAHDDAVTWLSAALTNHDTCAD 138
Query: 213 GFEDTSGTVKDQMVGNLKDLSELVSNSLAIFS--------ASGDNDFTGVPIQN------ 258
+ +L +V + L +++ S + GVP++N
Sbjct: 139 SLNEAGIPHGADAAPHLAAARAMVRDCLTMYAEAASAAMATSNKDGLAGVPVRNGGGKSK 198
Query: 259 KRRLMGMSDISREFPKWLEKRDRRLLSLPVSEI--QADIIVSKSGGNGTVKTITEAIKKA 316
K+R G FP+WL RDRRLL +P + + AD++V+K G GT +TI++A+K A
Sbjct: 199 KQRKRGRRRRRSLFPRWLSARDRRLLLVPAAPLVESADMVVAKDG-TGTHRTISDAVKAA 257
Query: 317 PEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTAS 376
PE S RR +I+V+AGRY+EN +KVG+KKTN++F+GDG+G TV++ RSV D TTFHTA+
Sbjct: 258 PERSGRRTVIHVKAGRYDEN-VKVGRKKTNLVFVGDGKGVTVVSAGRSVADNFTTFHTAT 316
Query: 377 FAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFR 436
FAASG GFM RD+T EN+AGPE+HQAVALRV +D A VYRC+I+GYQD Y HSNR F+R
Sbjct: 317 FAASGSGFMMRDMTVENWAGPERHQAVALRVSADRAAVYRCSIIGYQDTLYAHSNRHFYR 376
Query: 437 ECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILP 496
+C++YGTVDF+FGNAA V Q+CN+++R P+ QKNT+TAQNR+DP Q+TG+ IH CR++P
Sbjct: 377 DCDVYGTVDFVFGNAAAVLQRCNLWSRSPLPGQKNTVTAQNRRDPGQSTGLVIHACRVVP 436
Query: 497 APDLASSKGSI-----ETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYY 551
+P S+ ++ TYLGRPWK+YSR V M+SY+G HV P GWL WN FAL TLYY
Sbjct: 437 SPPPPSTAPAVAAPLAPTYLGRPWKLYSRVVVMMSYIGGHVPPEGWLAWNATFALDTLYY 496
Query: 552 GEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGLS 608
GEYMN+GPGA + RV WPG+RVI + EA R+TVA+FISG+SWLP+TGV+FL+GLS
Sbjct: 497 GEYMNYGPGAGVAGRVAWPGHRVINDSAEAERFTVARFISGASWLPATGVSFLSGLS 553
>gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis thaliana] gi|9279579|dbj|BAB01037.1|
pectinesterase [Arabidopsis thaliana]
gi|15529256|gb|AAK97722.1| AT3g14310/MLN21_9
[Arabidopsis thaliana] gi|14335010|gb|AAK59769.1|
AT3g14310/MLN21_9 [Arabidopsis thaliana]
gi|15231828|ref|NP_188048.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 592
Score = 479 bits (1233), Expect = e-133
Identities = 274/619 (44%), Positives = 367/619 (59%), Gaps = 59/619 (9%)
Query: 20 APLTPSTTSNSSPKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPT 79
AP S + K NKKL++LS A+L +A+ + + + AS N S +
Sbjct: 2 APSMKEIFSKDNFKKNKKLVLLSAAVALLFVAA------VAGISAGASKANEKRTLSPSS 55
Query: 80 QAISRT-CSKTRYPSLCINSLLDFPGSTSASEQELVHISFNMT-----HRH--ISKALFA 131
A+ R+ CS TRYP LCI++++ G S+++++ S N+T H + + K +
Sbjct: 56 HAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKK 115
Query: 132 SSGLSYTVANPRVRAAYEDCLELMDESMDAIRSSMDSL--MTTSSTLSNDDGESRQFSNV 189
GL+ PR + A DCLE +DE++D + +++ L T TL G
Sbjct: 116 RKGLT-----PREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAG-------- 162
Query: 190 AGSTEDVMTWLSAALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASG 247
D+ T +S+A+TNQ+TCL+GF +D V+ ++ + + SN+LA+
Sbjct: 163 -----DLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMT 217
Query: 248 DNDFTGVP-----IQNKRRLM-------------GMSDISRE-FPKWLEKRDRRLLSLPV 288
D D N R+L G ++ E +P WL DRRLL
Sbjct: 218 DTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQ--G 275
Query: 289 SEIQADIIVSKSGGNGTVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIM 348
S ++AD V+ + G+GT KT+ A+ APE+S +R++I+++AG Y EN ++V KKK NIM
Sbjct: 276 SGVKADATVA-ADGSGTFKTVAAAVAAAPENSNKRYVIHIKAGVYREN-VEVAKKKKNIM 333
Query: 349 FIGDGRGKTVITGKRSVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVG 408
F+GDGR +T+ITG R+V DG TTFH+A+ AA G F+ARDITF+N AGP KHQAVALRVG
Sbjct: 334 FMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVG 393
Query: 409 SDHAVVYRCNIVGYQDACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQ 468
SD + Y C+++ YQD YVHSNRQFF +C I GTVDFIFGNAAVV Q C+I+AR+P +
Sbjct: 394 SDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSG 453
Query: 469 QKNTITAQNRKDPNQNTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYM 528
QKN +TAQ R DPNQNTGI I CRI DL S KGS TYLGRPWK YS+TV M S +
Sbjct: 454 QKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAI 513
Query: 529 GDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQ 588
D + P GW EW G FAL TL Y EY N G GA RVKW G++VIT+ EA +YT Q
Sbjct: 514 SDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQ 573
Query: 589 FISGSSWLPSTGVAFLAGL 607
FI G WL STG F GL
Sbjct: 574 FIGGGGWLSSTGFPFSLGL 592
>emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]
Length = 579
Score = 479 bits (1232), Expect = e-133
Identities = 265/594 (44%), Positives = 371/594 (61%), Gaps = 36/594 (6%)
Query: 29 NSSPKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNL--LHSKPTQAISRTC 86
N + KK + L+++A+VL++A+ I + RS S +++++ + S + C
Sbjct: 7 NVNFSKGKKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSAAHAIVKSAC 66
Query: 87 SKTRYPSLC---INSLLDFPGSTSASEQELVHISFNMTHRHISKALFASSGLSYTVAN-- 141
T +P LC I S+ DF + S+++++ +S N+T R + F L T
Sbjct: 67 ENTLHPELCYSTIASVSDFSKKVT-SQKDVIELSLNITCRAVQHNFFKVEKLIKTRKGLK 125
Query: 142 PRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLS 201
PR + A DCLE +DE++D + +++ L + S + +D+ T +S
Sbjct: 126 PREKVALHDCLETIDETLDELHTAIKDLELYPNKKS-----------LKAHADDLKTLIS 174
Query: 202 AALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQNK 259
+A+TNQ+TCL+GF +D V+ ++ K + ++ SN+LA+ D D I N+
Sbjct: 175 SAITNQETCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTD-----IANE 229
Query: 260 RRLMG------MSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAI 313
++L G + + + E+P+WL DRRLL S ++ D++V+ + G+G KT++EA+
Sbjct: 230 QKLKGTTTNRKLREDNSEWPEWLSAGDRRLLQS--STVRPDVVVA-ADGSGNFKTVSEAV 286
Query: 314 KKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFH 373
KAPE S +R++I ++AG Y EN + V KKKTNIMF+GDGR T+ITG R+V DG TTFH
Sbjct: 287 AKAPEKSSKRYVIRIKAGVYREN-VDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFH 345
Query: 374 TASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQ 433
+A+ AA G F+ARDITF+N AG KHQAVALRVGSD + YRC+I+ YQD+ YVHSNRQ
Sbjct: 346 SATVAAVGEKFLARDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQ 405
Query: 434 FFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCR 493
+F +C I GTVDFIFGNAA V Q C+I+AR+P + QKN +TAQ R DPNQNTGI I CR
Sbjct: 406 YFVQCLIAGTVDFIFGNAAAVLQNCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCR 465
Query: 494 ILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGE 553
I DL + S TYLGRPWK YSRTV M S + D ++ GW EWNG+FAL TL+YGE
Sbjct: 466 IGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGE 525
Query: 554 YMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
Y N G GA RVKW G++VITS EA YT +FI+G SWL STG F GL
Sbjct: 526 YQNTGAGAGTSGRVKWKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579
>gb|AAM91439.1| At1g53830/T18A20_6 [Arabidopsis thaliana] gi|6056392|gb|AAF02856.1|
pectinesterase 2 [Arabidopsis thaliana]
gi|13605623|gb|AAK32805.1| At1g53830/T18A20_6
[Arabidopsis thaliana] gi|15220955|ref|NP_175786.1|
pectinesterase family protein [Arabidopsis thaliana]
gi|17865767|sp|Q42534|PME2_ARATH Pectinesterase-2
precursor (Pectin methylesterase 2) (PE 2)
Length = 587
Score = 476 bits (1225), Expect = e-133
Identities = 268/603 (44%), Positives = 361/603 (59%), Gaps = 32/603 (5%)
Query: 20 APLTPSTTSNSSPKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPT 79
AP+ + S K+NKKLI+ S A+L++AS + A T +++ + L S
Sbjct: 2 APIKEFISKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNK--NQKITTLSSTSH 59
Query: 80 QAISRTCSKTRYPSLCINSLLDFPGSTSASEQELVHISFNMTHRHISKALFASSGL--SY 137
+ CS T YP LC +++ G S++E++ S N+T + + FA L
Sbjct: 60 AILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKR 119
Query: 138 TVANPRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVM 197
PR A DCLE +DE++D + +++ L S + +D+
Sbjct: 120 KGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKS-----------LRKHADDLK 168
Query: 198 TWLSAALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVP 255
T +S+A+TNQ TCL+GF +D V+ ++ + + SN+LA+ + D
Sbjct: 169 TLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFE 228
Query: 256 IQNK---------RRLMGMS-DISRE-FPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNG 304
+++K R+L ++ D+ + +PKWL DRRLL S I+AD V+ G +G
Sbjct: 229 LRDKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ--GSTIKADATVADDG-SG 285
Query: 305 TVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRS 364
T+ A+ APE S +RF+I+++AG Y EN ++V KKKTNIMF+GDGRGKT+ITG R+
Sbjct: 286 DFTTVAAAVAAAPEKSNKRFVIHIKAGVYREN-VEVTKKKTNIMFLGDGRGKTIITGSRN 344
Query: 365 VGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQD 424
V DG TTFH+A+ AA G F+ARDITF+N AGP KHQAVALRVGSD + Y+C++ YQD
Sbjct: 345 VVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQD 404
Query: 425 ACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQN 484
YVHSNRQFF +C+I GTVDFIFGNAA V Q C+I AR+P + QKN +TAQ R DPNQN
Sbjct: 405 TLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQN 464
Query: 485 TGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDF 544
TGI I +CRI DL + KG+ TYLGRPWK YSRTV M S + D + P GW EW+G F
Sbjct: 465 TGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSF 524
Query: 545 ALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFL 604
AL TL Y EY+N G GA RVKW GY+VITS EA +T QFI G WL STG F
Sbjct: 525 ALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFS 584
Query: 605 AGL 607
L
Sbjct: 585 LSL 587
>gb|AAF23892.1| pectin methyl esterase [Solanum tuberosum]
Length = 576
Score = 476 bits (1224), Expect = e-132
Identities = 261/583 (44%), Positives = 366/583 (62%), Gaps = 31/583 (5%)
Query: 36 KKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLL--HSKPTQAISRTCSKTRYPS 93
KK I L+I+A+VL++A+ I RS S ++++++ S + CS T +P
Sbjct: 14 KKKIYLAIVASVLLVAAVIGVVAGVKSRSNNSDDDADIMAISSSAHAIVKSACSNTLHPE 73
Query: 94 LCINSLLDFPGSTS--ASEQELVHISFNMTHRHISKALFASSGLSYTVAN--PRVRAAYE 149
LC +++++ + S+++++ +S N+T + + + +A L T PR + A
Sbjct: 74 LCYSAIVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALH 133
Query: 150 DCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQDT 209
DCLE MDE++D + +++ L + S + ED+ T +S+A+TNQ+T
Sbjct: 134 DCLETMDETLDELHTAVADLELYPNKKSLKE-----------HAEDLKTLISSAITNQET 182
Query: 210 CLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQNKRRLMGMSD 267
CL+GF ++ V+ ++ K + ++ SN+LA+ + T I N+ +L G
Sbjct: 183 CLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMIC-----NMTNTDIANEMKLSGSRK 237
Query: 268 I---SREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKAPEHSRRRF 324
+ + E+P+WL DRRLL S + D++V+ + G+G KT++EA+ KAPE S +R+
Sbjct: 238 LVEDNGEWPEWLSAGDRRLLQS--STVTPDVVVA-ADGSGDYKTVSEAVAKAPEKSSKRY 294
Query: 325 IIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTASFAASGPGF 384
+I ++AG Y EN + V KKKTNIMF+GDGR T+IT R+V DG TTFH+A+ AA G F
Sbjct: 295 VIRIKAGVYREN-VDVPKKKTNIMFMGDGRSNTIITASRNVQDGSTTFHSATVAAVGEKF 353
Query: 385 MARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFRECNIYGTV 444
+ARDITF+N AG KHQAVALRVGSD + Y+C+I+ YQD YVHSNRQFF +C + GTV
Sbjct: 354 LARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVAGTV 413
Query: 445 DFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILPAPDLASSK 504
DFIFGN A V Q C+I+AR+P + QKN +TAQ R DPNQNTGI I CRI DL +
Sbjct: 414 DFIFGNGAAVLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQ 473
Query: 505 GSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAAIG 564
S TYLGRPWK YSRTV M S + D + P GW EWNG+FAL TL+YGEY N G GAA
Sbjct: 474 KSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGAATS 533
Query: 565 QRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
RVKW G++VITS+ EA YT FI+G SWL STG F GL
Sbjct: 534 GRVKWKGHKVITSSTEAQAYTPGSFIAGGSWLSSTGFPFSLGL 576
>gb|AAC72288.1| putative pectin methylesterase [Arabidopsis thaliana]
Length = 592
Score = 475 bits (1223), Expect = e-132
Identities = 272/619 (43%), Positives = 365/619 (58%), Gaps = 59/619 (9%)
Query: 20 APLTPSTTSNSSPKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPT 79
AP S + K NKKL++LS A+L +A+ + + + AS N S +
Sbjct: 2 APSMKEIFSKDNFKKNKKLVLLSAAVALLFVAA------VAGISAGASKANEKRTLSPSS 55
Query: 80 QAISRT-CSKTRYPSLCINSLLDFPGSTSASEQELVHISFNMT-----HRH--ISKALFA 131
A+ R+ CS TRYP LCI++++ S+++++ S N+T H + + K +
Sbjct: 56 HAVLRSSCSSTRYPELCISAVVTAGACELTSQKDVIEASVNLTITAVEHNYFTVKKLIKK 115
Query: 132 SSGLSYTVANPRVRAAYEDCLELMDESMDAIRSSMDSL--MTTSSTLSNDDGESRQFSNV 189
GL+ PR + A DCLE +DE++D + +++ L T TL G
Sbjct: 116 RKGLT-----PREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAG-------- 162
Query: 190 AGSTEDVMTWLSAALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASG 247
D+ T +S+A+TNQ+TCL+GF +D V+ ++ + + SN+LA+
Sbjct: 163 -----DLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMT 217
Query: 248 DNDFTGVP-----IQNKRRLM-------------GMSDISRE-FPKWLEKRDRRLLSLPV 288
D D N R+L G ++ E +P WL DRRLL
Sbjct: 218 DTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQ--G 275
Query: 289 SEIQADIIVSKSGGNGTVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIM 348
S ++ D V+ + G+GT KT+ A+ APE+S +R++I+++AG Y EN ++V KKK NIM
Sbjct: 276 SGVKRDATVA-ADGSGTFKTVAAAVAAAPENSNKRYVIHIKAGVYREN-VEVAKKKKNIM 333
Query: 349 FIGDGRGKTVITGKRSVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVG 408
F+GDGR +T+ITG R+V DG TTFH+A+ AA G F+ARDITF+N AGP KHQAVALRVG
Sbjct: 334 FMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVG 393
Query: 409 SDHAVVYRCNIVGYQDACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQ 468
SD + Y C+++ YQD YVHSNRQFF +C I GTVDFIFGNAAVV Q C+I+AR+P +
Sbjct: 394 SDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSG 453
Query: 469 QKNTITAQNRKDPNQNTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYM 528
QKN +TAQ R DPNQNTGI I CRI DL S KGS TYLGRPWK YS+TV M S +
Sbjct: 454 QKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAI 513
Query: 529 GDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQ 588
D + P GW EW G FAL TL Y EY N G GA RVKW G++VIT+ EA +YT Q
Sbjct: 514 SDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQ 573
Query: 589 FISGSSWLPSTGVAFLAGL 607
FI G WL STG F GL
Sbjct: 574 FIGGGGWLSSTGFPFSLGL 592
>gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana]
Length = 579
Score = 473 bits (1217), Expect = e-132
Identities = 263/594 (44%), Positives = 369/594 (61%), Gaps = 36/594 (6%)
Query: 29 NSSPKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNL--LHSKPTQAISRTC 86
N + KK + L+++A+VL++A+ I + RS S +++++ + S + C
Sbjct: 7 NVNFSKGKKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSAAHAIVKSAC 66
Query: 87 SKTRYPSLC---INSLLDFPGSTSASEQELVHISFNMTHRHISKALFASSGLSYTVAN-- 141
T +P LC I S+ DF + S+++++ +S N+T R + F L T
Sbjct: 67 ENTLHPELCYSTIASVSDFSKKVT-SQKDVIELSLNITCRAVQHNFFKVEKLIKTRKGLK 125
Query: 142 PRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLS 201
PR + A DCLE +DE++D + +++ L + S + + + T +S
Sbjct: 126 PREKVALHDCLETIDETLDELHTAIKDLELYPNKKS-----------LKAHADGLKTLIS 174
Query: 202 AALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQNK 259
+A+TNQ+TCL+GF +D V+ ++ K + ++ SN+LA+ D D I N+
Sbjct: 175 SAITNQETCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTD-----IANE 229
Query: 260 RRLMG------MSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAI 313
++L G + + + E+P+WL DRRLL S ++ D++V+ + G+G KT++EA+
Sbjct: 230 QKLKGTTTNRKLREDNSEWPEWLPAGDRRLLQS--STVRPDVVVA-ADGSGNFKTVSEAV 286
Query: 314 KKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFH 373
KAPE S +R++I ++AG Y EN + V KKKTNIMF+GDGR T+ITG R+V DG TTFH
Sbjct: 287 AKAPEKSSKRYVIRIKAGVYREN-VDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFH 345
Query: 374 TASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQ 433
+A+ AA G F+ARDITF+N AG KHQAVALRVGSD + YRC+I+ YQD+ YVHSNRQ
Sbjct: 346 SATVAAVGEKFLARDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQ 405
Query: 434 FFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCR 493
+F +C I GTVDFIFGNAA V Q C+I+AR+P + QKN +TAQ R DPNQNTGI I CR
Sbjct: 406 YFVQCLIAGTVDFIFGNAAAVLQDCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCR 465
Query: 494 ILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGE 553
I DL + S YLGRPWK YSRTV M S + D ++ GW EWNG+FAL TL+YGE
Sbjct: 466 IGATSDLRPVQKSFPMYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGE 525
Query: 554 YMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
Y N G GA RVKW G++VITS EA YT +FI+G SWL STG F GL
Sbjct: 526 YQNTGAGAGTSGRVKWKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579
>sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methylesterase 3) (PE 3)
Length = 584
Score = 472 bits (1214), Expect = e-131
Identities = 264/591 (44%), Positives = 365/591 (61%), Gaps = 29/591 (4%)
Query: 25 STTSNSS--PKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPTQAI 82
ST N S PK KKL L++ A +L++A+ I ++ V SR +S ++ ++P AI
Sbjct: 15 STNQNISNIPKKKKKLF-LALFATLLVVAAVIG--IVAGVNSRKNSGDNG---NEPHHAI 68
Query: 83 SRT-CSKTRYPSLCINSLLDFPGSTS--ASEQELVHISFNMTHRHISKALFASSGL-SYT 138
++ CS TRYP LC +++ P ++ S+++++ +S N+T + F L T
Sbjct: 69 LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128
Query: 139 VANPRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMT 198
R + A DCLE +DE++D + +++ L E +++ +D+ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLE-----------EYPNKKSLSQHADDLKT 177
Query: 199 WLSAALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPI 256
+SAA+TNQ TCL+GF +D + V+D + + ++ SN+LA+ D D +
Sbjct: 178 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 237
Query: 257 QNKRRLMGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKA 316
N R+L+ + +P WL DRRLL S + +++V+ + G+G KT+ ++ A
Sbjct: 238 SNNRKLIEETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVA-ADGSGNFKTVAASVAAA 294
Query: 317 PEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTAS 376
P+ +R+II ++AG Y EN ++V KK NIMFIGDGR +T+ITG R+V DG TTF +A+
Sbjct: 295 PQGGTKRYIIRIKAGVYREN-VEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 353
Query: 377 FAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFR 436
A G GF+ARDITF+N AGP KHQAVALRVG+D + Y C+++ YQD YVHSNRQFF
Sbjct: 354 VAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 413
Query: 437 ECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILP 496
C I GTVDFIFGNAA V Q C+I+ARKP + QKN +TAQ R DPNQNTGI I RI
Sbjct: 414 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGA 473
Query: 497 APDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMN 556
DL +GS TYLGRPWK YSRTV M S + D +HP GW EW+G+FAL TL+YGE+ N
Sbjct: 474 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQN 533
Query: 557 FGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
G GA RVKW G+RVITS EA +T FI+GSSWL STG F GL
Sbjct: 534 AGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>gb|AAC50023.1| ATPME2 precursor [Arabidopsis thaliana]
Length = 582
Score = 469 bits (1208), Expect = e-131
Identities = 264/590 (44%), Positives = 355/590 (59%), Gaps = 32/590 (5%)
Query: 33 KSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPTQAISRTCSKTRYP 92
K+NKKLI+ S A+L++AS + A T +++ + L S + CS T YP
Sbjct: 10 KNNKKLILSSAAIALLLLASIVGIAATTTNQNK--NQKITTLFSTSHAILKSVCSSTLYP 67
Query: 93 SLCINSLLDFPGSTSASEQELVHISFNMTHRHISKALFASSGL--SYTVANPRVRAAYED 150
LC +++ G S++E++ S N+T + + FA L PR A D
Sbjct: 68 ELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHD 127
Query: 151 CLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQDTC 210
CLE +DE++D + +++ L S + +D+ T +S+A+TNQ TC
Sbjct: 128 CLETIDETLDELHVAVEDLHQYPKQKS-----------LRKHADDLKTLISSAITNQGTC 176
Query: 211 LEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQNK--------- 259
L+GF +D V+ ++ + + SN+LA+ + D +++K
Sbjct: 177 LDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKFFNLHQQQQ 236
Query: 260 RRLMGMS-DISRE-FPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKAP 317
R+L ++ D+ + +PKWL DRRLL S I+AD V+ G +G + A+ AP
Sbjct: 237 RKLKEVTGDLDSDGWPKWLSVGDRRLLQ--GSTIKADATVADDG-SGDFDNGSAAVAAAP 293
Query: 318 EHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTASF 377
E S +RF+I+++AG Y EN ++V KKKTNIMF+GDGRGKT+ITG R+V DG TTFH+A+
Sbjct: 294 EKSNKRFVIHIKAGVYREN-VEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATV 352
Query: 378 AASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFRE 437
AA G F+ARDITF+N AGP KHQAVALRVGSD + Y+C++ YQD YVHSNRQFF +
Sbjct: 353 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVK 412
Query: 438 CNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILPA 497
C+I GTVDFIFGNAA V Q C+I AR+P + QKN +TAQ R DPNQNTGI I +CRI
Sbjct: 413 CHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGT 472
Query: 498 PDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMNF 557
DL + KG+ TYLGRPWK YSRTV M S + D + P GW EW+G FAL TL Y EY+N
Sbjct: 473 SDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNR 532
Query: 558 GPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
G GA RVKW GY+VITS EA +T QFI G WL STG F L
Sbjct: 533 GGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 582
>gb|AAB57670.1| pectinesterase [Citrus sinensis]
Length = 584
Score = 469 bits (1207), Expect = e-130
Identities = 261/582 (44%), Positives = 359/582 (60%), Gaps = 27/582 (4%)
Query: 32 PKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPTQAISRT-CSKTR 90
PK KKL L++ A +L++A+ I ++ V SR +S ++ ++P AI ++ CS TR
Sbjct: 24 PKKKKKLF-LALFATLLVVAAVIG--IVAGVNSRKNSGDNG---NEPHHAILKSSCSSTR 77
Query: 91 YPSLCINSLLDFPGSTS--ASEQELVHISFNMTHRHISKALFASSGL-SYTVANPRVRAA 147
YP LC +++ P ++ S+++++ +S N+T + F L T R + A
Sbjct: 78 YPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVA 137
Query: 148 YEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQ 207
DCLE +DE++D + +++ L E +++ +D+ T +SAA+TNQ
Sbjct: 138 LHDCLETIDETLDELHKAVEDLE-----------EYPNKKSLSQHADDLKTLMSAAMTNQ 186
Query: 208 DTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQNKRRLMGM 265
TCL+GF +D + V+D + + ++ SN+LA+ D D + N R+L
Sbjct: 187 GTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEE 246
Query: 266 SDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKAPEHSRRRFI 325
+ +P WL DRRLL S + + +V+ + G+G KT+ A+ AP+ +R+I
Sbjct: 247 TSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVA-ADGSGNFKTVAAAVAAAPQGGTKRYI 303
Query: 326 IYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTASFAASGPGFM 385
I ++AG Y EN ++V KK NIMFIGDGR +T+ITG R+V DG TTF +A+ A G GF+
Sbjct: 304 IRIKAGVYREN-VEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFL 362
Query: 386 ARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFRECNIYGTVD 445
ARDITF+N AGP KHQAVALRVG+D + Y C+++ YQD YVHSNRQFF C I GTVD
Sbjct: 363 ARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVD 422
Query: 446 FIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILPAPDLASSKG 505
FIFGNAA V Q C+I+ARKP + QKN +TAQ R DPNQNTGI I RI DL +G
Sbjct: 423 FIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQG 482
Query: 506 SIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAAIGQ 565
S TYLGRPWK YSRTV M S + D +HP GW EW+G+FAL TL+YGE+ N G GA
Sbjct: 483 SFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSG 542
Query: 566 RVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
RVKW G+RVITS EA +T FI+GSSWL STG F GL
Sbjct: 543 RVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methylesterase) (PE)
gi|2098705|gb|AAB57667.1| pectinesterase [Citrus
sinensis]
Length = 584
Score = 469 bits (1206), Expect = e-130
Identities = 261/582 (44%), Positives = 359/582 (60%), Gaps = 27/582 (4%)
Query: 32 PKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLLHSKPTQAISRT-CSKTR 90
PK KKL L++ A +L++A+ I ++ V SR +S ++ ++P AI ++ CS TR
Sbjct: 24 PKKKKKLF-LALFATLLVVAAVIG--IVAGVNSRKNSGDNG---NEPHHAILKSSCSSTR 77
Query: 91 YPSLCINSLLDFPGSTS--ASEQELVHISFNMTHRHISKALFASSGL-SYTVANPRVRAA 147
YP LC +++ P ++ S+++++ +S N+T + F L T R + A
Sbjct: 78 YPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVA 137
Query: 148 YEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQ 207
DCLE +DE++D + +++ L E +++ +D+ T +SAA+TNQ
Sbjct: 138 LHDCLETIDETLDELHKAVEDLE-----------EYPNKKSLSQHADDLKTLMSAAMTNQ 186
Query: 208 DTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQNKRRLMGM 265
TCL+GF +D + V+D + + ++ SN+LA+ D D + N R+L
Sbjct: 187 GTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEE 246
Query: 266 SDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKAPEHSRRRFI 325
+ +P WL DRRLL S + + +V+ + G+G KT+ A+ AP+ +R+I
Sbjct: 247 TSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVA-ADGSGNFKTVAAAVAAAPQGGTKRYI 303
Query: 326 IYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTASFAASGPGFM 385
I ++AG Y EN ++V KK NIMFIGDGR +T+ITG R+V DG TTF +A+ A G GF+
Sbjct: 304 IRIKAGVYREN-VEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFL 362
Query: 386 ARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFRECNIYGTVD 445
ARDITF+N AGP KHQAVALRVG+D + Y C+++ YQD YVHSNRQFF C I GTVD
Sbjct: 363 ARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVD 422
Query: 446 FIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILPAPDLASSKG 505
FIFGNAA V Q C+I+ARKP + QKN +TAQ R DPNQNTGI I RI DL +G
Sbjct: 423 FIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQG 482
Query: 506 SIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAAIGQ 565
S TYLGRPWK YSRTV M S + D +HP GW EW+G+FAL TL+YGE+ N G GA
Sbjct: 483 SFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSG 542
Query: 566 RVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
RVKW G+RVITS EA +T FI+GSSWL STG F GL
Sbjct: 543 RVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>emb|CAE76634.1| pectin methylesterase [Cicer arietinum]
Length = 254
Score = 464 bits (1194), Expect = e-129
Identities = 214/254 (84%), Positives = 236/254 (92%)
Query: 356 KTVITGKRSVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVY 415
KTVITG ++V +TTFHTASFAASGPGF+ARD+TFENYAGP KHQAVALRVG+DHAVVY
Sbjct: 1 KTVITGGKNVMQNLTTFHTASFAASGPGFIARDVTFENYAGPAKHQAVALRVGADHAVVY 60
Query: 416 RCNIVGYQDACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITA 475
RCNI+GYQD YVHSNRQF+REC+IYGTVDFIFGNAAVVFQ C++YARKPMAQQKNTITA
Sbjct: 61 RCNIIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITA 120
Query: 476 QNRKDPNQNTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPH 535
QNRKDPNQNTGISIH+CRIL PDL +SKGS +TYLGRPWK+YS+TVYMLSYMGDH+HP
Sbjct: 121 QNRKDPNQNTGISIHNCRILATPDLEASKGSFQTYLGRPWKLYSKTVYMLSYMGDHIHPR 180
Query: 536 GWLEWNGDFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSW 595
GWLEWN FAL TLYYGEYMN+GPG AIGQRV WPG+RVITST+EANR+TVAQFISGSSW
Sbjct: 181 GWLEWNATFALDTLYYGEYMNYGPGGAIGQRVTWPGFRVITSTVEANRFTVAQFISGSSW 240
Query: 596 LPSTGVAFLAGLST 609
LPSTGVAF+AGLST
Sbjct: 241 LPSTGVAFVAGLST 254
>gb|AAL02367.1| pectin methylesterase [Lycopersicon esculentum]
gi|1222552|gb|AAD09283.1| pectin methylesterase
[Lycopersicon esculentum] gi|7447374|pir||T07848
pectinesterase (EC 3.1.1.11) - tomato
gi|6093740|sp|Q43143|PMEU_LYCES Pectinesterase U1
precursor (Pectin methylesterase) (PE)
Length = 583
Score = 463 bits (1192), Expect = e-129
Identities = 253/585 (43%), Positives = 363/585 (61%), Gaps = 32/585 (5%)
Query: 36 KKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLL--HSKPTQAISRTCSKTRYPS 93
KK I L+I+A+VL++A+ I S+ S ++++++ S + CS T +P
Sbjct: 18 KKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHPE 77
Query: 94 LCINSLLDFPGSTS--ASEQELVHISFNMTHRHISKALFASSGLSYTVAN--PRVRAAYE 149
LC +++++ + S+++++ +S N+T + + + +A L T PR + A
Sbjct: 78 LCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALH 137
Query: 150 DCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALTNQDT 209
DCLE MDE++D + ++++ L + S + ED+ T +S+A+TNQ+T
Sbjct: 138 DCLETMDETLDELHTAVEDLELYPNKKSLKE-----------HVEDLKTLISSAITNQET 186
Query: 210 CLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTG-----VPIQNKRRL 262
CL+GF ++ V+ ++ K + ++ SN+LA+ D D P N++
Sbjct: 187 CLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRK-- 244
Query: 263 MGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKAPEHSRR 322
+ + + E+P+WL DRRLL S + D++V+ + G+G KT++EA++KAPE S +
Sbjct: 245 --LVEDNGEWPEWLSAGDRRLLQS--STVTPDVVVA-ADGSGDYKTVSEAVRKAPEKSSK 299
Query: 323 RFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTASFAASGP 382
R++I ++AG Y EN + V KKKTNIMF+GDG+ T+IT R+V DG TTFH+A+
Sbjct: 300 RYVIRIKAGVYREN-VDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAG 358
Query: 383 GFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFRECNIYG 442
+ARDITF+N AG KHQAVAL VGSD + YRC+++ YQD YVHSNRQFF +C + G
Sbjct: 359 KVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAG 418
Query: 443 TVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILPAPDLAS 502
TVDFIFGN A VFQ C+I+AR+P + QKN +TAQ R DPNQNTGI I CRI DL
Sbjct: 419 TVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRP 478
Query: 503 SKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGAA 562
+ S TYLGRPWK YSRTV M S + D + P GW EWNG+FAL TL+YGEY N G GA
Sbjct: 479 VQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAP 538
Query: 563 IGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
RVKW G++VITS+ EA YT +FI+G SWL STG F GL
Sbjct: 539 TSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
>gb|AAK84486.1| putative thermostable pectinesterase [Citrus sinensis]
gi|24250746|gb|AAK84485.1| putative thermostable
pectinesterase [Citrus sinensis]
Length = 631
Score = 462 bits (1189), Expect = e-128
Identities = 255/603 (42%), Positives = 365/603 (60%), Gaps = 31/603 (5%)
Query: 17 RSTAPLTPSTTSNSSPKSNKKLIMLSILAAVLIIASAISAALITVVRSRASSNNSNLL-- 74
R+ A T T+ + P+ + L++ A VL++ + ++ +I ++ +S+ N +
Sbjct: 48 RNQALSTKIFTTMAQPRK----LFLALFAVVLVVTAVVT--IIATRKNNSSNTNDEHIAA 101
Query: 75 --HSKPTQAISRTCSKTRYPSLCINSLLDFPGSTSA---SEQELVHISFNMTHRHISKAL 129
H+ I +CS T YP LC ++L P + + S ++++ +S N+T +
Sbjct: 102 HAHAYAQPVIRSSCSATLYPELCFSALSAAPAAAVSKVNSPKDVIRLSLNLTITAVQHNY 161
Query: 130 FASSGLSYT---VANPRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQF 186
FA L T R + + DCLE++DE++D + + L + +N
Sbjct: 162 FAIKKLITTRKSTLTKREKTSLHDCLEMVDETLDELYKTEHELQGYPAAANN-------- 213
Query: 187 SNVAGSTEDVMTWLSAALTNQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFS 244
++A +++ +SAA+TNQ+TCL+GF E ++++++ + + SN+LA+
Sbjct: 214 KSIAEQADELKILVSAAMTNQETCLDGFSHERADKKIREELMEGQMHVFHMCSNALAMIK 273
Query: 245 ASGDNDFTGVPIQNKRRLMGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNG 304
D D + + + + D ++ +P+WL DRRLL + + D+ V+ + G+G
Sbjct: 274 NMTDGDIGKDIVDHYSKARRLDDETK-WPEWLSAGDRRLLQ--ATTVVPDVTVA-ADGSG 329
Query: 305 TVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRS 364
T+ A+ APE S RR+II ++AG Y EN ++V KKK N+MFIGDGR T+ITG R+
Sbjct: 330 NYLTVAAAVAAAPEGSSRRYIIRIKAGEYREN-VEVPKKKINLMFIGDGRTTTIITGSRN 388
Query: 365 VGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQD 424
V DG TTF++A+ A G GF+ARDITF+N AGP KHQAVALRVGSD + YRC+++ YQD
Sbjct: 389 VVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQD 448
Query: 425 ACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQN 484
YVHS RQF+ C I GTVDFIFGNAA VFQ C+I+AR+P Q+N +TAQ R DPNQN
Sbjct: 449 TLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQN 508
Query: 485 TGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDF 544
TGI I CRI DL + KGS ETYLGRPWK YSRTV M S + D ++P GW EW+G+F
Sbjct: 509 TGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNF 568
Query: 545 ALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFL 604
AL TL+Y EY N G GA RVKW ++VITS EA YT A FI+GS+WL STG F
Sbjct: 569 ALDTLFYAEYQNTGAGADTSNRVKWSTFKVITSAAEAQTYTAANFIAGSTWLGSTGFPFS 628
Query: 605 AGL 607
GL
Sbjct: 629 LGL 631
>emb|CAE76633.2| pectin methylesterase [Cicer arietinum]
Length = 584
Score = 457 bits (1177), Expect = e-127
Identities = 257/586 (43%), Positives = 358/586 (60%), Gaps = 27/586 (4%)
Query: 34 SNKKLIMLSILAAVLIIAS--AISAALITVVRSRASSN--NSNLLHSKPTQAISRT-CSK 88
SNKK + S+ A+LI AS AISA++ + +++ +N +S+L S + I +T C+
Sbjct: 14 SNKKKLFFSLFTALLITASIIAISASVTSSQKTKTKNNIVSSSLSLSHHSHTIIKTACTT 73
Query: 89 TRYPSLCINSLLDFPGSTSA--SEQELVHISFNMTHRHISKALFASSGLSYTV-ANPRVR 145
T YP LC +++ P T + ++++ +S N+T R + F L + R +
Sbjct: 74 TLYPDLCFSAISSEPNITHKINNHKDVISLSLNITTRAVEHNFFTVENLLRRKNLSEREK 133
Query: 146 AAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGESRQFSNVAGSTEDVMTWLSAALT 205
A DCLE +D+++D ++ + L+ + + +D+ T +SAA+T
Sbjct: 134 IALHDCLETIDDTLDELKEAQRDLVLYPNK-----------KTLYQHADDLKTLISAAIT 182
Query: 206 NQDTCLEGF--EDTSGTVKDQMVGNLKDLSELVSNSLAIFSASGDNDFTGVPIQN--KRR 261
NQ TCL+GF + V+ + + + SN+LA+ D D N K R
Sbjct: 183 NQVTCLDGFSHDGADKQVRKVLEQGQVHVEHMCSNALAMTKNMTDKDIAKFEENNNKKNR 242
Query: 262 LMGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGNGTVKTITEAIKKAPEHSR 321
+ + +P+W+ DRRLL + ++AD++V+ + G+G KT++EA+ AP S
Sbjct: 243 KLLEEENGVNWPEWISAGDRRLLQ--GAAVKADVVVA-ADGSGNFKTVSEAVAGAPLKSS 299
Query: 322 RRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKRSVGDGMTTFHTASFAASG 381
+R++I ++AG Y+EN ++V KKK+NIMF+GDG+ T+IT R+V DG TTFH+A+ A G
Sbjct: 300 KRYVIKIKAGVYKEN-VEVPKKKSNIMFLGDGKKNTIITASRNVVDGSTTFHSATVAVVG 358
Query: 382 PGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQDACYVHSNRQFFRECNIY 441
F+ARDITF+N AGP KHQAVALRVG D + Y C+I+ YQD YVH+NRQFF C I
Sbjct: 359 GNFLARDITFQNTAGPSKHQAVALRVGGDLSAFYNCDIIAYQDTLYVHNNRQFFVNCFIS 418
Query: 442 GTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQNTGISIHDCRILPAPDLA 501
GTVDFIFGN+AVVFQ C+I+ARKP + QKN +TAQ R DPNQNTGI I CRI DL
Sbjct: 419 GTVDFIFGNSAVVFQNCDIHARKPDSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATKDLE 478
Query: 502 SSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNGDFALKTLYYGEYMNFGPGA 561
KG+ TYLGRPWK YSRTV M S + D + P GW EWNG+FAL TL Y EY N GPGA
Sbjct: 479 GLKGTFPTYLGRPWKEYSRTVIMQSSISDVIDPIGWHEWNGNFALNTLVYREYQNTGPGA 538
Query: 562 AIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTGVAFLAGL 607
+RV W G++VITS EA +T FI GS+WL STG F GL
Sbjct: 539 GTSKRVNWKGFKVITSASEAQTFTPGNFIGGSTWLGSTGFPFSLGL 584
>dbj|BAD45460.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
Length = 426
Score = 450 bits (1157), Expect = e-125
Identities = 230/428 (53%), Positives = 288/428 (66%), Gaps = 19/428 (4%)
Query: 197 MTWLSAALTNQDTCLEGFEDTSGT-VKDQMVGNLKDLSELVSNSLAIF------------ 243
MT LSAA+TNQ TCL+GF+ G V+ M ++ +S +VSNSLA+
Sbjct: 1 MTELSAAMTNQYTCLDGFDYKDGERVRHYMESSIHHVSRMVSNSLAMAKKLPGAGGGGMT 60
Query: 244 --SASGDNDFTGVPIQNKRR--LMGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSK 299
S+S D + +R MG ++ FPKW+ DRRLL P S I D +V+K
Sbjct: 61 PSSSSPDTATQSESSETTQRQPFMGYGQMANGFPKWVRPGDRRLLQAPASSITPDAVVAK 120
Query: 300 SGGNGTVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVI 359
G G T++ A+ AP +S +R++I+++AG Y EN ++VGK K N+MFIGDG GKTVI
Sbjct: 121 DGSGGYT-TVSAAVAAAPANSNKRYVIHIKAGAYMEN-VEVGKSKKNLMFIGDGIGKTVI 178
Query: 360 TGKRSVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNI 419
R+V DG TTF +A+ A G F+ARD+T EN AGP KHQAVALRVG+D + YRC+
Sbjct: 179 KASRNVVDGSTTFRSATVAVVGNNFLARDLTIENSAGPSKHQAVALRVGADLSAFYRCSF 238
Query: 420 VGYQDACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRK 479
VGYQD YVHS RQFFREC+IYGT+DFIFGN+AVVFQ CN+YAR+P+ Q N TAQ R+
Sbjct: 239 VGYQDTLYVHSLRQFFRECDIYGTIDFIFGNSAVVFQSCNLYARRPLPNQSNVYTAQGRE 298
Query: 480 DPNQNTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLE 539
DPNQNTGISI C++ A DL + + S +TYLGRPWK YSRTV+M S + V+P GWLE
Sbjct: 299 DPNQNTGISIQKCKVAAASDLLAVQSSFKTYLGRPWKQYSRTVFMQSELDSVVNPAGWLE 358
Query: 540 WNGDFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPST 599
W+G+FAL TLYYGEY N GPGA+ RVKW GYRVITS EA+ +TV FI G WL T
Sbjct: 359 WSGNFALDTLYYGEYQNTGPGASTSNRVKWKGYRVITSASEASTFTVGNFIDGDVWLAGT 418
Query: 600 GVAFLAGL 607
V F GL
Sbjct: 419 SVPFTVGL 426
>dbj|BAC67661.1| pectin methylesterase [Pisum sativum]
Length = 554
Score = 449 bits (1156), Expect = e-125
Identities = 235/487 (48%), Positives = 319/487 (65%), Gaps = 33/487 (6%)
Query: 124 HISKALFASSGLSYTVANPRVRAAYEDCLELMDESMDAIRSSMDSLMTTSSTLSNDDGES 183
HI KA+ ++ + V +PR A DC +LMD SMD + DS++T +
Sbjct: 98 HIQKAMETANVIKRRVNSPREETALNDCEQLMDLSMDRV---WDSVLTLTK--------- 145
Query: 184 RQFSNVAGSTEDVMTWLSAALTNQDTCLEGFEDTSGTVKDQMVGNLKDLSELVSNSLAIF 243
N S +D TWLS+ LTN TCL G E TS V M G+L+DL +SLA+
Sbjct: 146 ----NNIDSQQDAHTWLSSVLTNHATCLNGLEGTSRVV---MEGDLQDLISRARSSLAVL 198
Query: 244 SASGDNDFTGVPIQNKRRLMGMSDISREFPKWLEKRDRRLLSLPVSEIQADIIVSKSGGN 303
+ +P ++ + S ++ EFP W+ +DRRLL V +I+A+++V+K G +
Sbjct: 199 VSV-------LPAKSNDGFIDES-LNGEFPSWVTSKDRRLLESTVGDIKANVVVAKDG-S 249
Query: 304 GTVKTITEAIKKAPEHSRRRFIIYVRAGRYEENNLKVGKKKTNIMFIGDGRGKTVITGKR 363
G KT+ EA+ AP++ + R++IYV+ G Y+E +++GKKKTN+M +GDG T+ITG
Sbjct: 250 GKFKTVAEAVASAPDNGKARYVIYVKRGTYKEK-VEIGKKKTNVMLVGDGMDATIITGNL 308
Query: 364 SVGDGMTTFHTASFAASGPGFMARDITFENYAGPEKHQAVALRVGSDHAVVYRCNIVGYQ 423
+ DG TTF++A+ AA G GF+A+DI F+N AGPEKHQAVALRVG+D +V+ RC I +Q
Sbjct: 309 NFIDGTTTFNSATVAAVGDGFIAQDIGFQNTAGPEKHQAVALRVGADQSVINRCKIDAFQ 368
Query: 424 DACYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTITAQNRKDPNQ 483
D Y HSNRQF+R+ I GTVDFIFGNA VVFQK + ARKPM+ QKN +TAQ R+DPNQ
Sbjct: 369 DTLYAHSNRQFYRDSFITGTVDFIFGNAGVVFQKSKLVARKPMSNQKNMVTAQGREDPNQ 428
Query: 484 NTGISIHDCRILPAPDLASSKGSIETYLGRPWKMYSRTVYMLSYMGDHVHPHGWLEWNG- 542
NT SI C ++P+ DL +GSI+TYLGRPWK YSRTV + S + H+ P GW EW+
Sbjct: 429 NTATSIQQCNVIPSSDLKPVQGSIKTYLGRPWKKYSRTVVLQSVVDSHIDPAGWAEWDAA 488
Query: 543 --DFALKTLYYGEYMNFGPGAAIGQRVKWPGYRVITSTLEANRYTVAQFISGSSWLPSTG 600
DF L+TLYYGEY+N G GA +RV WPGY +I + EA+++TV Q I G+ WL +TG
Sbjct: 489 SKDF-LQTLYYGEYLNSGAGAGTSKRVTWPGYHIIKTAAEASKFTVTQLIQGNVWLKNTG 547
Query: 601 VAFLAGL 607
VAF+ GL
Sbjct: 548 VAFIEGL 554
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.317 0.131 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,020,867,419
Number of Sequences: 2540612
Number of extensions: 42628728
Number of successful extensions: 121990
Number of sequences better than 10.0: 483
Number of HSP's better than 10.0 without gapping: 342
Number of HSP's successfully gapped in prelim test: 142
Number of HSP's that attempted gapping in prelim test: 120357
Number of HSP's gapped (non-prelim): 634
length of query: 609
length of database: 863,360,394
effective HSP length: 134
effective length of query: 475
effective length of database: 522,918,386
effective search space: 248386233350
effective search space used: 248386233350
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 78 (34.7 bits)
Medicago: description of AC134822.9