BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.22 + phase: 0 /pseudo
(516 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_198963.2| dihydrofolate synthetase/folylpolyglutamate syn... 348 2e-94
emb|CAC80838.1| dihydrofolate synthetase /folylpolyglutamate syn... 307 5e-82
dbj|BAB08519.1| folylpolyglutamate synthase-like protein [Arabid... 286 1e-75
gb|AAP40483.1| putative folylpolyglutamate synthase [Arabidopsis... 158 4e-37
emb|CAI44282.1| folylpolyglutamate synthase/dihydrofolate syntha... 119 2e-25
emb|CAI44426.1| folylpolyglutamate synthase/dihydrofolate syntha... 115 3e-24
ref|NP_227981.1| folylpolyglutamate synthase/dihydrofolate synth... 114 7e-24
pdb|1O5Z|A Chain A, Crystal Structure Of Folylpolyglutamate Synt... 114 7e-24
ref|NP_470922.1| folC [Listeria innocua Clip11262] gi|16414073|e... 114 1e-23
gb|AAT74583.1| dihydrofolate/folylpolyglutamate synthase [Toxopl... 112 4e-23
ref|ZP_00327343.1| COG0285: Folylpolyglutamate synthase [Trichod... 111 5e-23
emb|CAI44326.1| folylpolyglutamate synthase/dihydrofolate syntha... 111 5e-23
emb|CAI44242.1| folylpolyglutamate synthase/dihydrofolate syntha... 111 5e-23
ref|NP_390686.1| folyl-polyglutamate synthetase [Bacillus subtil... 111 6e-23
ref|NP_465076.1| hypothetical protein lmo1551 [Listeria monocyto... 111 6e-23
gb|EAA15667.1| dihydrofolate synthase/folylpolyglutamate synthas... 111 6e-23
emb|CAH99321.1| dihydrofolate synthase/folylpolyglutamate syntha... 110 8e-23
ref|ZP_00111127.2| COG0285: Folylpolyglutamate synthase [Nostoc ... 110 1e-22
ref|YP_171308.1| folylpolyglutamate synthase [Synechococcus elon... 107 1e-21
ref|ZP_00510488.1| Folylpolyglutamate synthetase [Clostridium th... 106 2e-21
>ref|NP_198963.2| dihydrofolate synthetase/folylpolyglutamate synthetase (DHFS/FPGS1)
[Arabidopsis thaliana]
Length = 530
Score = 348 bits (894), Expect = 2e-94
Identities = 235/556 (42%), Positives = 320/556 (57%), Gaps = 101/556 (18%)
Query: 2 RSVRTLCSHREDSEMKDLVDYIDSLKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGNP 61
R++ T+ S ED E++D V +++SLKNYEK GVP GAGTDS+DGFDLGRM+RL+ R NP
Sbjct: 32 RNLSTISS-TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNP 90
Query: 62 HSKFKTE----------------------GYSVGCYSSPHIQTIRERILGRSGDPVSAKL 99
H K+K GYSVGCYSSPHI +I+ERI +G+PVSA
Sbjct: 91 HYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERI-SCNGEPVSAST 149
Query: 100 LNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAVIEAGLGGARDATNI 159
LN+LF+ +K L+Q+I+EENG +SHFE+ T +AF LF E VDIAVIEAGLGGARDATN+
Sbjct: 150 LNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNV 209
Query: 160 ISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLGGPFLPHIEYIIREK 219
I SS LAA+VITT+GEEH+AALGGSLESIA AK+GIIK G P+VLGGPFLPHIE I+R K
Sbjct: 210 IESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSK 269
Query: 220 AVNMDSPVVSASDSGNNLAVKSFSILNG-KPCQICDIEIQTVK---------DLKLAMDP 269
A ++ S V+ AS+ G++ ++K NG CQ CDI IQ K D+ L M
Sbjct: 270 AASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLG 329
Query: 270 DPRCACGPVSCMI*SC----KCLVLTN----FKMQQLLLV*HSVFVI*VDGEFLMNL*GV 321
+ + + C C +T+ ++ L+ S F+ + E L+ L G
Sbjct: 330 HHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLL-LPGA 388
Query: 322 G*SVHTSLEGVSS*NLKKPRPWDFLEQQYCLI*SVAHTKESAKALMNTIKMASPKARLAF 381
+ + S+ LK+ DF E++ + + MAS K ++F
Sbjct: 389 TVLLDGAHTKESARALKEMIKKDFPEKRLVFV----------------VAMASDKDHVSF 432
Query: 382 VVAMASDKDHAGFAREILSDAYVKTVILTEAAIAGAVTRTAPASLLRDSWIKASEELGTD 441
A+E+LS + VILTEA I G R+ +S+L++SWIKA++ELG
Sbjct: 433 -------------AKELLSGLKPEAVILTEADIGGGKIRSTESSVLKESWIKAADELG-- 477
Query: 442 ICHDGMTEYRELFKEQPVSSESNLTDGKTILATESSLKDCLRMANEIL--NRRRDEKGVI 499
S ++ KT+L + L++A +IL + + G++
Sbjct: 478 ------------------SRSMEASENKTVLGS-------LKLAYKILSDDTTSSDSGMV 512
Query: 500 VITGSLHIVSSVLASL 515
++TGSLHIVSSVLASL
Sbjct: 513 IVTGSLHIVSSVLASL 528
>emb|CAC80838.1| dihydrofolate synthetase /folylpolyglutamate synthetase
[Arabidopsis thaliana]
Length = 530
Score = 307 bits (787), Expect = 5e-82
Identities = 166/284 (58%), Positives = 206/284 (72%), Gaps = 25/284 (8%)
Query: 2 RSVRTLCSHREDSEMKDLVDYIDSLKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGNP 61
R++ T+ S ED E++D V +++SLKNYEK GVP GAGTDS+DGFDLGRM+RL+ R NP
Sbjct: 32 RNLSTISS-TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNP 90
Query: 62 HSKFKTE----------------------GYSVGCYSSPHIQTIRERILGRSGDPVSAKL 99
H K+K GYSVGCYSSPHI +I+ERI +G+PVSA
Sbjct: 91 HYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERI-SCNGEPVSAST 149
Query: 100 LNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAVIEAGLGGARDATNI 159
LN+LF+ +K L+Q+I+EENG +SHFE+ T +AF LF E VDIAVIEAGLGGARDATN+
Sbjct: 150 LNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNV 209
Query: 160 ISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLGGPFLPHIEYIIREK 219
I SS LAA+VITT+GEEH+AALGGSLESIA AK+GIIK G P+VLGGPFLPHIE I+R K
Sbjct: 210 IESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSK 269
Query: 220 AVNMDSPVVSASDSGNNLAVKSFSILNG-KPCQICDIEIQTVKD 262
A ++ S V+ AS+ G++ ++K NG CQ CDI IQ KD
Sbjct: 270 AASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKD 313
Score = 117 bits (293), Expect = 9e-25
Identities = 72/161 (44%), Positives = 96/161 (58%), Gaps = 29/161 (18%)
Query: 357 AHTKESAKALMNTIKMASPKARLAFVVAMASDKDHAGFAREILSDAYVKTVILTEAAIAG 416
AHTKESA+AL IK P+ RL FVVAMASDKDH FA+E+LS + VILTEA I G
Sbjct: 395 AHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGG 454
Query: 417 AVTRTAPASLLRDSWIKASEELGTDICHDGMTEYRELFKEQPVSSESNLTDGKTILATES 476
R+ +S L++SWIKA++ELG S ++ KT+L +
Sbjct: 455 GKIRSTESSALKESWIKAADELG--------------------SRSMEASENKTVLGS-- 492
Query: 477 SLKDCLRMANEIL--NRRRDEKGVIVITGSLHIVSSVLASL 515
L++A +IL + + G++++TGSLHIVSSVLASL
Sbjct: 493 -----LKLAYKILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528
>dbj|BAB08519.1| folylpolyglutamate synthase-like protein [Arabidopsis thaliana]
Length = 480
Score = 286 bits (731), Expect = 1e-75
Identities = 210/556 (37%), Positives = 292/556 (51%), Gaps = 132/556 (23%)
Query: 2 RSVRTLCSHREDSEMKDLVDYIDSLKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGNP 61
R++ T+ S ED E++D V +++SLKNYEK GVP GAGTDS+DGFDLGRM+RL+ R NP
Sbjct: 13 RNLSTISS-TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNP 71
Query: 62 HSKFKTE----------------------GYSVGCYSSPHIQTIRERILGRSGDPVSAKL 99
H K+K GYSVGCYSSPHI +I+ERI +G+PVSA
Sbjct: 72 HYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERI-SCNGEPVSAST 130
Query: 100 LNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAVIEAGLGGARDATNI 159
LN+LF+ +K L+Q+I+EENG +SHFE+ T +AF LF E VDIAVIEAGLGGARDATN+
Sbjct: 131 LNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNV 190
Query: 160 ISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLGGPFLPHIEYIIREK 219
I SS LAA+VITT+ GGPFLPHIE I+R K
Sbjct: 191 IESSNLAASVITTI-------------------------------GGPFLPHIEGILRSK 219
Query: 220 AVNMDSPVVSASDSGNNLAVKSFSILNG-KPCQICDIEIQTVK---------DLKLAMDP 269
A ++ S V+ AS+ G++ ++K NG CQ CDI IQ K D+ L M
Sbjct: 220 AASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLG 279
Query: 270 DPRCACGPVSCMI*SC----KCLVLTN----FKMQQLLLV*HSVFVI*VDGEFLMNL*GV 321
+ + + C C +T+ ++ L+ S F+ + E L+ L G
Sbjct: 280 HHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLL-LPGA 338
Query: 322 G*SVHTSLEGVSS*NLKKPRPWDFLEQQYCLI*SVAHTKESAKALMNTIKMASPKARLAF 381
+ + S+ LK+ DF E++ + + MAS K ++F
Sbjct: 339 TVLLDGAHTKESARALKEMIKKDFPEKRLVFV----------------VAMASDKDHVSF 382
Query: 382 VVAMASDKDHAGFAREILSDAYVKTVILTEAAIAGAVTRTAPASLLRDSWIKASEELGTD 441
A+E+LS + VILTEA I G R+ +S+L++SWIKA++ELG
Sbjct: 383 -------------AKELLSGLKPEAVILTEADIGGGKIRSTESSVLKESWIKAADELG-- 427
Query: 442 ICHDGMTEYRELFKEQPVSSESNLTDGKTILATESSLKDCLRMANEIL--NRRRDEKGVI 499
S ++ KT+L + L++A +IL + + G++
Sbjct: 428 ------------------SRSMEASENKTVLGS-------LKLAYKILSDDTTSSDSGMV 462
Query: 500 VITGSLHIVSSVLASL 515
++TGSLHIVSSVLASL
Sbjct: 463 IVTGSLHIVSSVLASL 478
>gb|AAP40483.1| putative folylpolyglutamate synthase [Arabidopsis thaliana]
Length = 318
Score = 158 bits (399), Expect = 4e-37
Identities = 131/373 (35%), Positives = 191/373 (51%), Gaps = 77/373 (20%)
Query: 163 SGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLGGPFLPHIEYIIREKAVN 222
S LAA+VITT+GEEH+AALGGSLESIA AK+GIIK G P+VLGGPFLPHI+ I+R KA +
Sbjct: 1 SSLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIKGILRSKAAS 60
Query: 223 MDSPVVSASDSGNNLAVKSFSILNG-KPCQICDIEIQTVK---------DLKLAMDPDPR 272
+ S V+ AS+ G++ ++K NG CQ CDI IQ K D+ L M +
Sbjct: 61 VSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQ 120
Query: 273 CACGPVSCMI*SC----KCLVLTN----FKMQQLLLV*HSVFVI*VDGEFLMNL*GVG*S 324
+ + C C +T+ ++ L+ S F+ + E L+ L G
Sbjct: 121 LQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLL-LPGATVL 179
Query: 325 VHTSLEGVSS*NLKKPRPWDFLEQQYCLI*SVAHTKESAKALMNTIKMASPKARLAFVVA 384
+ + S+ LK+ DF E++ + + MAS K ++F
Sbjct: 180 LDGAHTKESARALKEMIKKDFPEKRLVFV----------------VAMASDKDHVSF--- 220
Query: 385 MASDKDHAGFAREILSDAYVKTVILTEAAIAGAVTRTAPASLLRDSWIKASEELGTDICH 444
A+E+LS + VILTEA I G R+ +S+L++SWIKA++ELG
Sbjct: 221 ----------AKELLSGLKPEAVILTEADIGGGKIRSTESSVLKESWIKAADELG----- 265
Query: 445 DGMTEYRELFKEQPVSSESNLTDGKTILATESSLKDCLRMANEIL--NRRRDEKGVIVIT 502
S ++ KT+L + L++A +IL + + G++++T
Sbjct: 266 ---------------SRSMEASENKTVLGS-------LKLAYKILSDDTTSSDSGMVIVT 303
Query: 503 GSLHIVSSVLASL 515
GSLHIVSSVLASL
Sbjct: 304 GSLHIVSSVLASL 316
>emb|CAI44282.1| folylpolyglutamate synthase/dihydrofolate synthase [Thermotoga
naphthophila]
Length = 437
Score = 119 bits (299), Expect = 2e-25
Identities = 73/180 (40%), Positives = 102/180 (56%), Gaps = 26/180 (14%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
L R+ LL + GNPH K+KT +GY VG Y SPH+ T RE
Sbjct: 30 LERISMLLSKLGNPHLKYKTIHVGGTNGKGSVTNMISHILISQGYRVGSYYSPHLSTFRE 89
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI + + +S + + ++ +++ L++ KEE S FEV TAMAF+ FA++ VDIAV
Sbjct: 90 RIR-LNEEYISEEDVVKIYEKMEPILNELDKEEIFSPSFFEVVTAMAFLYFAEKNVDIAV 148
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
+E GLGG DATN++ + I TV +H LG ++E IA K+GIIK+G PLV G
Sbjct: 149 LEVGLGGRLDATNVVFP---LCSTIVTVDRDHEKTLGYTIEQIAWEKSGIIKEGVPLVTG 205
>emb|CAI44426.1| folylpolyglutamate synthase/dihydrofolate synthase [Thermotoga sp.
RQ2] gi|61657383|emb|CAI44299.1| folylpolyglutamate
synthase/dihydrofolate synthase [Thermotoga petrophila]
Length = 437
Score = 115 bits (288), Expect = 3e-24
Identities = 76/201 (37%), Positives = 110/201 (53%), Gaps = 29/201 (14%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
L R+ LL + GNPH K+K+ +GY VG Y SPH+ T RE
Sbjct: 30 LERISMLLSKLGNPHLKYKSIHVGGTNGKGSVTNMISHILISQGYRVGSYYSPHLSTFRE 89
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI + + +S + + ++ +++ L++ KEE S FEV TAMAF+ FA++ VDIAV
Sbjct: 90 RIR-LNEEYISEEDVVKIYEKMEPILNELDKEEIFSPSFFEVVTAMAFLYFAEKNVDIAV 148
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
+E GLGG DATN++ + I TV +H LG ++E IA K+GIIK+ PLV G
Sbjct: 149 LEVGLGGRLDATNVVFP---LCSTIVTVDRDHEKTLGYTIEQIAWEKSGIIKERVPLVTG 205
Query: 206 ---GPFLPHIEYIIREKAVNM 223
L +E + R+K+ M
Sbjct: 206 ERKREALKVMEDVARKKSSRM 226
>ref|NP_227981.1| folylpolyglutamate synthase/dihydrofolate synthase [Thermotoga
maritima MSB8] gi|4980660|gb|AAD35259.1|
folylpolyglutamate synthase/dihydrofolate synthase
[Thermotoga maritima MSB8] gi|7437972|pir||D72411
folylpolyglutamate synthase/dihydrofolate synthase -
Thermotoga maritima (strain MSB8)
Length = 430
Score = 114 bits (285), Expect = 7e-24
Identities = 76/201 (37%), Positives = 109/201 (53%), Gaps = 29/201 (14%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
L R+ LL + GNPH ++KT +GY VG Y SPH+ T RE
Sbjct: 23 LERISMLLSKLGNPHLEYKTIHIGGTNGKGSVANMVSNILVSQGYRVGSYYSPHLSTFRE 82
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI + + +S + + ++ ++ L++ KEE S FEV TAMAF+ FA++ VDIAV
Sbjct: 83 RIR-LNEEYISEEDVVKIYETMEPILNELDKEEIFSPSFFEVVTAMAFLYFAEKNVDIAV 141
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
+E GLGG DATN++ + I TV +H LG ++E IA K+GIIK+ PLV G
Sbjct: 142 LEVGLGGRLDATNVVFP---LCSTIVTVDRDHEKTLGYTIEQIAWEKSGIIKERVPLVTG 198
Query: 206 ---GPFLPHIEYIIREKAVNM 223
L +E + R+K+ M
Sbjct: 199 ERKREALKVMEDVARKKSSRM 219
>pdb|1O5Z|A Chain A, Crystal Structure Of Folylpolyglutamate Synthase (Tm0166)
From Thermotoga Maritima At 2.10 A Resolution
Length = 442
Score = 114 bits (285), Expect = 7e-24
Identities = 76/201 (37%), Positives = 109/201 (53%), Gaps = 29/201 (14%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
L R+ LL + GNPH ++KT +GY VG Y SPH+ T RE
Sbjct: 35 LERISMLLSKLGNPHLEYKTIHIGGTNGKGSVANMVSNILVSQGYRVGSYYSPHLSTFRE 94
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI + + +S + + ++ ++ L++ KEE S FEV TAMAF+ FA++ VDIAV
Sbjct: 95 RIR-LNEEYISEEDVVKIYETMEPILNELDKEEIFSPSFFEVVTAMAFLYFAEKNVDIAV 153
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
+E GLGG DATN++ + I TV +H LG ++E IA K+GIIK+ PLV G
Sbjct: 154 LEVGLGGRLDATNVVFP---LCSTIVTVDRDHEKTLGYTIEQIAWEKSGIIKERVPLVTG 210
Query: 206 ---GPFLPHIEYIIREKAVNM 223
L +E + R+K+ M
Sbjct: 211 ERKREALKVMEDVARKKSSRM 231
>ref|NP_470922.1| folC [Listeria innocua Clip11262] gi|16414073|emb|CAC96817.1| folC
[Listeria innocua] gi|25293648|pir||AI1630
Folyl-polyglutamate synthetase homolog folC [imported] -
Listeria innocua (strain Clip11262)
Length = 429
Score = 114 bits (284), Expect = 1e-23
Identities = 69/166 (41%), Positives = 100/166 (59%), Gaps = 6/166 (3%)
Query: 63 SKFKTEGYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCI 122
S + GY G ++SP+I+T ERI +G PVS +++ +L +RIK ++ K G
Sbjct: 62 SSLEEAGYKTGTFTSPYIETFNERI-SVNGIPVSDQMIVDLANRIKPIAEELEKTVYGPP 120
Query: 123 SHFEVFTAMAFILFAD-EKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAAL 181
S FE+ TAM F+ FA+ E +DI +IE GLGG D+TN+++ +VITT+G +H+ L
Sbjct: 121 SEFEIITAMMFLCFAEYESIDIGIIEVGLGGRLDSTNVLTP---LISVITTIGMDHMEFL 177
Query: 182 GGSLESIAMAKAGIIKQGCPLVLGGPFLPHIEYIIREKAVNMDSPV 227
G S+E IA KAGIIK G P V+ G + +I AV +S V
Sbjct: 178 GNSIEQIAGEKAGIIKPGIP-VISGVIQKEAQEVIINNAVRNNSNV 222
>gb|AAT74583.1| dihydrofolate/folylpolyglutamate synthase [Toxoplasma gondii]
Length = 553
Score = 112 bits (279), Expect = 4e-23
Identities = 75/198 (37%), Positives = 107/198 (53%), Gaps = 15/198 (7%)
Query: 68 EGYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEV 127
+GY VG Y+SPHI + RERI S + + + +L+HR+ Q Q E ++ FEV
Sbjct: 70 KGYKVGTYTSPHILSFRERIRVDS-QMIPEEDVVSLYHRVTQTAKQMGVE----LTFFEV 124
Query: 128 FTAMAFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLES 187
T MAF+ F+ ++VD AVIE GLGG DATNI+ S VIT++G +H+ LG +L+
Sbjct: 125 TTQMAFLYFSQQEVDWAVIETGLGGRLDATNIVDSP--RCTVITSIGWDHMNVLGDTLDR 182
Query: 188 IAMAKAGIIKQGCPLVLGGPFLPHIEYIIREKAVNMDSPVVSASDSGNNLAVKSFSILNG 247
IA KAG+IK PLVLG H + + V D V A G + ++
Sbjct: 183 IAQEKAGVIKAKTPLVLGPTAAVHPCFRQKAACVGADVMEVQAEPRGEDFEDENI----- 237
Query: 248 KPCQICDIEIQTVKDLKL 265
+IC + ++ V L L
Sbjct: 238 ---RICRVVVENVLRLDL 252
>ref|ZP_00327343.1| COG0285: Folylpolyglutamate synthase [Trichodesmium erythraeum
IMS101]
Length = 420
Score = 111 bits (278), Expect = 5e-23
Identities = 83/266 (31%), Positives = 128/266 (47%), Gaps = 49/266 (18%)
Query: 23 IDS-LKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGNPH------------------- 62
IDS LK +E +GV L R+ +LLD G PH
Sbjct: 4 IDSFLKRFEHFGVE----------LSLERIYQLLDNLGKPHLQVPIIHVAGTNGKGSVCA 53
Query: 63 ---SKFKTEGYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEEN 119
S K GY VG Y+SPH+ ERI K+ +++ ++ +++ AI+ E
Sbjct: 54 YLSSILKAAGYKVGRYTSPHLVDWTERICLNE-----EKIPSSILEQLLVEVETAIQPEQ 108
Query: 120 GCISHFEVFTAMAFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLA 179
+ FEV TA A++ FA ++VD+AV+E GLGG DATN+ VIT++ EH
Sbjct: 109 PSPTQFEVITAAAWLYFAQQQVDVAVVEVGLGGRLDATNVCHQP--MVTVITSISWEHWQ 166
Query: 180 ALGGSLESIAMAKAGIIKQGCPLVLGGPFLPHIEYIIREKAVNMDSPVVSA--------S 231
LG +L IA KAGI+K CP ++ G P + ++ ++ +++ PVV A S
Sbjct: 167 KLGPTLSHIAGEKAGILKPRCPAIV-GVLPPEAKKVVEKRITDLECPVVWAEPAKLLAES 225
Query: 232 DSGNNLAVKSFSILNGKPCQICDIEI 257
N+L V ++ + K D+++
Sbjct: 226 QEYNSLPVATYQGIEYKVPLFGDVQL 251
>emb|CAI44326.1| folylpolyglutamate synthase/dihydrofolate synthase [Thermotoga sp.
RQ7]
Length = 430
Score = 111 bits (278), Expect = 5e-23
Identities = 78/243 (32%), Positives = 122/243 (50%), Gaps = 31/243 (12%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
L R+ LL GNPH+K++ +GY VG Y SPH+ T RE
Sbjct: 23 LERISLLLSLLGNPHTKYRVIHVGGTNGKGSVTNMISNVLIAQGYRVGSYYSPHLSTFRE 82
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI + + +S + + ++ +++ L + + E S FE TAMAF+ FA++ VD+AV
Sbjct: 83 RIR-LNEEFISEEDVERIYQQMEPVLKRLDENEVFSPSFFETVTAMAFLYFAEKNVDVAV 141
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
+E GLGG DATN++S ++I TV +H LG ++E IA K+GIIK P+V G
Sbjct: 142 VEVGLGGRLDATNVVSP---LCSIIVTVDRDHEKVLGTTIEQIAWEKSGIIKNSVPVVTG 198
Query: 206 GPFLPHIEYIIREKAVNMDSPVVSASDSGNNLAVKSFSILNGKPCQICDIEIQTVKDLKL 265
++ I E+ V A + + V+S + + + D + +KDL L
Sbjct: 199 EEKTEALKVI--EEVARKKGSKVYALNRDFAVEVESLKLFENRFSYLGD---KVLKDLVL 253
Query: 266 AMD 268
M+
Sbjct: 254 TMN 256
>emb|CAI44242.1| folylpolyglutamate synthase/dihydrofolate synthase [Thermotoga
neapolitana]
Length = 431
Score = 111 bits (278), Expect = 5e-23
Identities = 78/243 (32%), Positives = 122/243 (50%), Gaps = 31/243 (12%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
L R+ LL GNPH+K++ +GY VG Y SPH+ T RE
Sbjct: 23 LERISLLLSLLGNPHTKYRVIHVGGTNGKGSVTNMISNVLIAQGYRVGSYYSPHLSTFRE 82
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI + + +S + + ++ +++ L + + E S FE TAMAF+ FA++ VD+AV
Sbjct: 83 RIR-LNEEFISEEDVERIYQQMEPVLKRLDENEVFSPSFFETVTAMAFLYFAEKNVDVAV 141
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
+E GLGG DATN++S ++I TV +H LG ++E IA K+GIIK P+V G
Sbjct: 142 VEVGLGGRLDATNVVSP---LCSIIVTVDRDHEKVLGTTIEQIAWEKSGIIKNSVPVVTG 198
Query: 206 GPFLPHIEYIIREKAVNMDSPVVSASDSGNNLAVKSFSILNGKPCQICDIEIQTVKDLKL 265
++ I E+ V A + + V+S + + + D + +KDL L
Sbjct: 199 EEKTEALKVI--EEVARKKGSKVYALNRDFAVEVESLKLFENRFSYLGD---KVLKDLVL 253
Query: 266 AMD 268
M+
Sbjct: 254 TMN 256
>ref|NP_390686.1| folyl-polyglutamate synthetase [Bacillus subtilis subsp. subtilis
str. 168] gi|2635273|emb|CAB14768.1| folyl-polyglutamate
synthetase [Bacillus subtilis subsp. subtilis str. 168]
gi|585155|sp|Q05865|FOLC_BACSU Folylpolyglutamate
synthase (Folylpoly-gamma-glutamate synthetase) (FPGS)
(Tetrahydrofolylpolyglutamate synthase)
gi|142936|gb|AAB59021.1| folyl-polyglutamate synthetase
Length = 430
Score = 111 bits (277), Expect = 6e-23
Identities = 76/226 (33%), Positives = 115/226 (50%), Gaps = 38/226 (16%)
Query: 31 KYGVPTGAGTDSNDGFDLGRMRRLLDRFGNPHSKFKT----------------------E 68
K+GV G LGRM++L+ R G+P K +
Sbjct: 17 KFGVKPG----------LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEA 66
Query: 69 GYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVF 128
GY+VG ++SP+I T ERI +G P+S + L +++K ++ + E G + FE+
Sbjct: 67 GYTVGTFTSPYIITFNERI-SVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIM 125
Query: 129 TAMAFILFAD-EKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLES 187
TA AF+ FA+ KVD + E GLGG D+TN++ VIT++G +H+ LG ++E
Sbjct: 126 TACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEP---LLTVITSIGHDHMNILGNTIEE 182
Query: 188 IAMAKAGIIKQGCPLVLGGPFLPHIEYIIREKAVNMDSPVVSASDS 233
IA KAGIIK+G P+V P +IR +A +P S D+
Sbjct: 183 IAGEKAGIIKEGIPIVT-AVTQPEALQVIRHEAERHAAPFQSLHDA 227
>ref|NP_465076.1| hypothetical protein lmo1551 [Listeria monocytogenes EGD-e]
gi|16410980|emb|CAC99629.1| folC [Listeria
monocytogenes] gi|47096716|ref|ZP_00234301.1|
folylpolyglutamate synthase [Listeria monocytogenes str.
1/2a F6854] gi|47014900|gb|EAL05848.1|
folylpolyglutamate synthase [Listeria monocytogenes str.
1/2a F6854] gi|25293647|pir||AG1268 Folyl-polyglutamate
synthetase homolog folC [imported] - Listeria
monocytogenes (strain EGD-e)
Length = 429
Score = 111 bits (277), Expect = 6e-23
Identities = 68/179 (37%), Positives = 105/179 (57%), Gaps = 14/179 (7%)
Query: 69 GYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVF 128
GY G ++SP+I+ ERI +G+P+S +++ +L +RIK ++ K G S FE+
Sbjct: 68 GYKAGTFTSPYIEVFNERI-SVNGEPISDEMIVSLANRIKPIAEELEKTVYGPPSEFEII 126
Query: 129 TAMAFILFADE-KVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLES 187
TAM F+ FAD +DI +IE GLGG D+TNI+ +VITT+G +H+ LG ++E
Sbjct: 127 TAMMFLCFADYVSIDIGIIEVGLGGRLDSTNILVP---LVSVITTIGMDHMEFLGDTIEQ 183
Query: 188 IAMAKAGIIKQGCPLVLGGPFLPHIEYIIREKAVNMDSPVVSASDSGNNLAVKSFSILN 246
IA KAGIIK G P+V G I+++A + + + + + S K+FS+ N
Sbjct: 184 IASEKAGIIKPGIPVVSGA---------IQKEAQEVIAEIATKNKSNVTQLNKAFSMHN 233
>gb|EAA15667.1| dihydrofolate synthase/folylpolyglutamate synthase [Plasmodium
yoelii yoelii]
Length = 491
Score = 111 bits (277), Expect = 6e-23
Identities = 73/228 (32%), Positives = 118/228 (51%), Gaps = 36/228 (15%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
LG ++L + FGNP+ +KT + Y VG +SSPHI ++RE
Sbjct: 25 LGSTKKLSELFGNPYKNYKTIHVAGTNGKGSVCHKIYLGLKLKKYKVGIFSSPHIFSLRE 84
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI+ + +P+S N+L + + +A K+ N + FE+ T +AFI F+++ VD AV
Sbjct: 85 RII-INDEPISE---NDLIALVNEVFKKA-KKINASPTFFEIITLVAFIYFSNQNVDYAV 139
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
IE GLGG DATNI+ L +IT++G +HL LG L I K GI K+ +++G
Sbjct: 140 IETGLGGRLDATNILEKPEL--VIITSIGYDHLNILGNDLNQICNEKIGIFKKDAQVIIG 197
Query: 206 GPFLPHIEYIIREKAVNMDSPVV-------SASDSGNNLAVKSFSILN 246
++ + K +N + +V + ++ +A++S ILN
Sbjct: 198 PSVSIYVNVFEKAKELNCNMHIVPPEPRGETYNEENTRIAIESLKILN 245
>emb|CAH99321.1| dihydrofolate synthase/folylpolyglutamate synthase, putative
[Plasmodium berghei]
Length = 472
Score = 110 bits (276), Expect = 8e-23
Identities = 74/228 (32%), Positives = 117/228 (50%), Gaps = 36/228 (15%)
Query: 48 LGRMRRLLDRFGNPHSKFKT----------------------EGYSVGCYSSPHIQTIRE 85
LG ++L + FGNP+ +KT + Y VG +SSPHI ++RE
Sbjct: 25 LGCTKKLSELFGNPYKNYKTIHVAGTNGKGSVCHKIYLGLKLKNYKVGIFSSPHIFSLRE 84
Query: 86 RILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGCISHFEVFTAMAFILFADEKVDIAV 145
RI+ +P+S N+L + + +A K+ N + FE+ T +AFI F+++ VD AV
Sbjct: 85 RIIVND-EPISE---NDLIALVNEVFKKA-KKINSSPTFFEIITLVAFIYFSNKNVDYAV 139
Query: 146 IEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKAGIIKQGCPLVLG 205
IE GLGG DATNI+ L +IT++G +HL LG L I K GI K+ +++G
Sbjct: 140 IETGLGGRLDATNILEKPEL--VIITSIGYDHLNILGNDLNQICNEKIGIFKKDAQVIIG 197
Query: 206 GPFLPHIEYIIREKAVNMDSPVV-------SASDSGNNLAVKSFSILN 246
+I + K +N + +V + ++ +A++S ILN
Sbjct: 198 PSVSIYINVFEKAKELNCNIHIVPPEPRGETYNEENTRIAIESLKILN 245
>ref|ZP_00111127.2| COG0285: Folylpolyglutamate synthase [Nostoc punctiforme PCC 73102]
Length = 432
Score = 110 bits (274), Expect = 1e-22
Identities = 78/229 (34%), Positives = 119/229 (51%), Gaps = 43/229 (18%)
Query: 23 IDS-LKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGNPH------------------- 62
IDS ++ ++++GV G L R+ LL GNPH
Sbjct: 3 IDSVIQPFQRFGVHLG----------LDRIINLLANLGNPHHQVPVIHVAGTNGKGSVCA 52
Query: 63 --SKFKTE-GYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEEN 119
S TE GY G Y+SPH+ ERI + P+S++ L+ L +++ I+ E+
Sbjct: 53 YLSSILTEAGYRTGRYTSPHLVDWTERIC-LNEQPISSEELSQLLEKVQG----VIRPED 107
Query: 120 GCISHFEVFTAMAFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLA 179
+ FEV TA A++ FA ++VD+AV+E GLGG DATN+ +IT++ EH
Sbjct: 108 ESATQFEVITAAAWLYFAQQQVDVAVVEVGLGGRLDATNVCLEP--IVTIITSISREHWQ 165
Query: 180 ALGGSLESIAMAKAGIIKQGCPLVLGGPFLP-HIEYIIREKAVNMDSPV 227
LG ++ IA KAGI+K GCP+V+G LP E ++R +A+ + P+
Sbjct: 166 QLGPTVADIAREKAGILKPGCPVVVGQ--LPLDAEEVVRSRALELKCPI 212
>ref|YP_171308.1| folylpolyglutamate synthase [Synechococcus elongatus PCC 6301]
gi|56685566|dbj|BAD78788.1| folylpolyglutamate synthase
[Synechococcus elongatus PCC 6301]
gi|46129590|ref|ZP_00202094.1| COG0285:
Folylpolyglutamate synthase [Synechococcus elongatus PCC
7942]
Length = 412
Score = 107 bits (266), Expect = 1e-21
Identities = 74/216 (34%), Positives = 115/216 (52%), Gaps = 36/216 (16%)
Query: 39 GTDSNDGFDLGRMRRL--LDRFGNPHSKF----------------------KTEGYSVGC 74
G+ + G DLG R L L+R GNP ++ + GY VG
Sbjct: 11 GSFAQRGVDLGLDRSLTVLERLGNPQNQIPIVHIAGTNGKGSTCAFVDTALRANGYRVGR 70
Query: 75 YSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENGC-ISHFEVFTAMAF 133
Y+SPH+ + ER L +G VS+ R+ + + + +G ++ FE+ TA +
Sbjct: 71 YTSPHLISWCER-LQLNGQQVSSD-------RLLEAVQTVLAAADGIDLTPFELLTAAVW 122
Query: 134 ILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMAKA 193
++ AD +DI VIE GLGG DATN+++ + A IT++G +H A LG +L +IA KA
Sbjct: 123 LVLADSDLDIVVIETGLGGRLDATNVVAQPLVTA--ITSIGHDHQAVLGPTLGAIAAEKA 180
Query: 194 GIIKQGCPLVLGGPFLPHIEYIIREKAVNMDSPVVS 229
GIIK CP+V+ GP LP +IR +A + + +++
Sbjct: 181 GIIKSRCPVVV-GPLLPEARTVIRNRAEQLQALMIA 215
>ref|ZP_00510488.1| Folylpolyglutamate synthetase [Clostridium thermocellum ATCC 27405]
gi|67849251|gb|EAM44868.1| Folylpolyglutamate synthetase
[Clostridium thermocellum ATCC 27405]
Length = 428
Score = 106 bits (265), Expect = 2e-21
Identities = 70/204 (34%), Positives = 104/204 (50%), Gaps = 35/204 (17%)
Query: 23 IDSLKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGNPHSKFK---------------- 66
++ + K+G+ G L +R+LL+ NPH K K
Sbjct: 7 LEYIHGTHKFGIKLG----------LDNIRKLLEFMDNPHRKLKYVHVAGTNGKGSTVAF 56
Query: 67 ------TEGYSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQAIKEENG 120
GY VG Y+SP+I+ ERI + D +S + L + +K ++ I +
Sbjct: 57 ISSILKESGYKVGIYTSPYIERFTERIK-INNDEISKEDLARITEYVKGKVELMISQGEN 115
Query: 121 CISHFEVFTAMAFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAA 180
+ FE+ TA+AF F ++ DI V+E GLGG D+TN+I S AAVITT+ +H+A
Sbjct: 116 HPTEFEIVTAIAFQYFYEKDCDIVVLEVGLGGRFDSTNVIDSP--LAAVITTISYDHMAQ 173
Query: 181 LGGSLESIAMAKAGIIKQGCPLVL 204
LG +L+ IA KAGIIK+G +VL
Sbjct: 174 LGDTLDKIAFEKAGIIKRGTDVVL 197
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.327 0.140 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 791,482,698
Number of Sequences: 2540612
Number of extensions: 31673241
Number of successful extensions: 102650
Number of sequences better than 10.0: 429
Number of HSP's better than 10.0 without gapping: 359
Number of HSP's successfully gapped in prelim test: 70
Number of HSP's that attempted gapping in prelim test: 101226
Number of HSP's gapped (non-prelim): 680
length of query: 516
length of database: 863,360,394
effective HSP length: 133
effective length of query: 383
effective length of database: 525,458,998
effective search space: 201250796234
effective search space used: 201250796234
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 77 (34.3 bits)
Medicago: description of AC124963.22