BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122728.13 + phase: 0
(164 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_849312.1| hypothetical protein [Arabidopsis thaliana] 169 3e-41
ref|XP_466725.1| unknown protein [Oryza sativa (japonica cultiva... 79 4e-14
gb|EAL90822.1| YKL529 [Aspergillus fumigatus Af293] 44 0.001
gb|EAA65661.1| hypothetical protein AN0831.2 [Aspergillus nidula... 44 0.001
emb|CAE72564.1| Hypothetical protein CBG19748 [Caenorhabditis br... 43 0.004
emb|CAD01107.1| conserved hypothetical protein [Neurospora crass... 42 0.005
pir||T48825 hypothetical protein 68B2.90 [imported] - Neurospora... 42 0.005
gb|EAA67313.1| hypothetical protein FG01852.1 [Gibberella zeae P... 41 0.010
emb|CAG84154.1| unnamed protein product [Yarrowia lipolytica CLI... 41 0.010
gb|EAK86445.1| hypothetical protein UM05579.1 [Ustilago maydis 5... 41 0.010
gb|EAA55403.1| hypothetical protein MG09210.4 [Magnaporthe grise... 41 0.013
gb|EAL18533.1| hypothetical protein CNBJ1750 [Cryptococcus neofo... 40 0.023
gb|AAS51641.1| ADL279Wp [Ashbya gossypii ATCC 10895] gi|45187594... 40 0.030
ref|XP_650388.1| SPRY domain protein [Entamoeba histolytica HM-1... 40 0.030
gb|AAX70633.1| hypothetical protein, conserved [Trypanosoma brucei] 39 0.039
emb|CAG59231.1| unnamed protein product [Candida glabrata CBS138... 39 0.039
ref|XP_637470.1| hypothetical protein DDB0187197 [Dictyostelium ... 39 0.051
emb|CAA89807.1| unknown [Saccharomyces cerevisiae] 38 0.11
ref|NP_013894.1| Endosomal protein of unknown function, mRNA is ... 38 0.11
gb|EAL30393.1| GA21506-PA [Drosophila pseudoobscura] 37 0.15
>ref|NP_849312.1| hypothetical protein [Arabidopsis thaliana]
Length = 169
Score = 169 bits (427), Expect = 3e-41
Identities = 88/173 (50%), Positives = 114/173 (65%), Gaps = 28/173 (16%)
Query: 1 MKLVFESEKAEWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAAN 60
MKLV ESEK EW +KV+GCG+DPR+KKV+FT++S L+HV++C+ +EF TPLYPTLA+N
Sbjct: 4 MKLVCESEKHEWAKENKVVGCGYDPRKKKVYFTVNSHLVHVINCKADEFGTPLYPTLASN 63
Query: 61 IDIMVLVNFGQNAFKYAPANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGRIDSHWL 120
+ VLVN GQ+ F Y+PAN QRT NPC A+ +DSKELFS+GRIDSH+L
Sbjct: 64 TEARVLVNLGQSPFYYSPANGQRTSNPC--------AGAVAGLEDSKELFSIGRIDSHYL 115
Query: 121 N---------------RTTTKGSHNNGNNISTMDFDEESEADLFEIVIDGSRK 158
+ R T SH +D+DEES+ADLFEI ++ S K
Sbjct: 116 SNFFINRGNSDEIASGRATVGNSHRR-----KLDYDEESDADLFEIALERSGK 163
>ref|XP_466725.1| unknown protein [Oryza sativa (japonica cultivar-group)]
gi|47497662|dbj|BAD19730.1| unknown protein [Oryza
sativa (japonica cultivar-group)]
gi|47496811|dbj|BAD19455.1| unknown protein [Oryza
sativa (japonica cultivar-group)]
Length = 91
Score = 79.0 bits (193), Expect = 4e-14
Identities = 39/74 (52%), Positives = 51/74 (68%), Gaps = 6/74 (8%)
Query: 53 LYPTLAANIDIMVLVNFGQNAFKYAPANAQRTPNPCLVSPLVS-----SPAAIGYD-DDS 106
+YP LA++ D+M LVN GQ F+YAPANA+RT NPC + + S ++G D DDS
Sbjct: 1 MYPVLASSFDVMALVNLGQGKFRYAPANARRTANPCFLRAASAGDDGRSGGSLGLDFDDS 60
Query: 107 KELFSMGRIDSHWL 120
+LFSMGR+DS WL
Sbjct: 61 GDLFSMGRVDSGWL 74
>gb|EAL90822.1| YKL529 [Aspergillus fumigatus Af293]
Length = 608
Score = 44.3 bits (103), Expect = 0.001
Identities = 27/68 (39%), Positives = 39/68 (56%), Gaps = 6/68 (8%)
Query: 18 VIGCGFDPRQKKVFFTLDSE-LMHVVH-CQTEEFSTPLYPTLAANIDIMVLVNFGQNAFK 75
V+G G+ PR +FFT + + L VVH +T+ F +PT+ AN V VNFGQ F
Sbjct: 288 VVGVGYRPRSGTIFFTRNGKKLEDVVHGAKTQNF----FPTVGANGPCTVHVNFGQMGFV 343
Query: 76 YAPANAQR 83
+ AN ++
Sbjct: 344 FIEANVKK 351
>gb|EAA65661.1| hypothetical protein AN0831.2 [Aspergillus nidulans FGSC A4]
gi|67517103|ref|XP_658435.1| hypothetical protein
AN0831_2 [Aspergillus nidulans FGSC A4]
gi|49085742|ref|XP_404968.1| hypothetical protein
AN0831.2 [Aspergillus nidulans FGSC A4]
Length = 502
Score = 44.3 bits (103), Expect = 0.001
Identities = 27/67 (40%), Positives = 37/67 (54%), Gaps = 4/67 (5%)
Query: 18 VIGCGFDPRQKKVFFTLDSE-LMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKY 76
V+G G+ PR +FFT + + L VVH Q S +PT+ AN V VNFGQ F +
Sbjct: 286 VVGVGYRPRSGTIFFTRNGKKLEDVVHGQK---SQNFFPTVGANGPCTVHVNFGQLGFVF 342
Query: 77 APANAQR 83
AN ++
Sbjct: 343 IEANVKK 349
>emb|CAE72564.1| Hypothetical protein CBG19748 [Caenorhabditis briggsae]
Length = 566
Score = 42.7 bits (99), Expect = 0.004
Identities = 25/64 (39%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Query: 14 GTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANI-DIMVLVNFGQN 72
G VIGCG D VFFT D EL+ + H LYPT+ + NFGQ
Sbjct: 116 GKHDVIGCGIDTNLNIVFFTKDGELLGIAHEGKPGEMENLYPTVGLRTPGEKLYANFGQY 175
Query: 73 AFKY 76
FK+
Sbjct: 176 PFKF 179
>emb|CAD01107.1| conserved hypothetical protein [Neurospora crassa]
gi|32404734|ref|XP_322980.1| hypothetical protein
[Neurospora crassa] gi|28923000|gb|EAA32218.1|
hypothetical protein [Neurospora crassa]
Length = 500
Score = 42.4 bits (98), Expect = 0.005
Identities = 26/68 (38%), Positives = 40/68 (58%), Gaps = 6/68 (8%)
Query: 18 VIGCGFDPRQKKVFFTLDSE-LMHVVH-CQTEEFSTPLYPTLAANIDIMVLVNFGQNAFK 75
VIG G+ PR +FFT + + L VVH +++ F +P++ AN +V VNFGQ F
Sbjct: 220 VIGVGYRPRSGTIFFTRNGKKLEDVVHGLKSQNF----FPSIGANGPCIVHVNFGQAGFV 275
Query: 76 YAPANAQR 83
+ AN ++
Sbjct: 276 FIEANVKK 283
>pir||T48825 hypothetical protein 68B2.90 [imported] - Neurospora crassa
Length = 503
Score = 42.4 bits (98), Expect = 0.005
Identities = 26/68 (38%), Positives = 40/68 (58%), Gaps = 6/68 (8%)
Query: 18 VIGCGFDPRQKKVFFTLDSE-LMHVVH-CQTEEFSTPLYPTLAANIDIMVLVNFGQNAFK 75
VIG G+ PR +FFT + + L VVH +++ F +P++ AN +V VNFGQ F
Sbjct: 223 VIGVGYRPRSGTIFFTRNGKKLEDVVHGLKSQNF----FPSIGANGPCIVHVNFGQAGFV 278
Query: 76 YAPANAQR 83
+ AN ++
Sbjct: 279 FIEANVKK 286
>gb|EAA67313.1| hypothetical protein FG01852.1 [Gibberella zeae PH-1]
gi|46109940|ref|XP_382028.1| hypothetical protein
FG01852.1 [Gibberella zeae PH-1]
Length = 522
Score = 41.2 bits (95), Expect = 0.010
Identities = 37/126 (29%), Positives = 58/126 (45%), Gaps = 18/126 (14%)
Query: 18 VIGCGFDPRQKKVFFTLD-SELMHVVH-CQTEEFSTPLYPTLAANIDIMVLVNFGQNAFK 75
V+G G+ PR +FFT + +L VVH + + F +P + AN + VN GQ F
Sbjct: 223 VVGVGYRPRTGAIFFTRNGKKLEEVVHGLKAQNF----FPAIGANGPATIHVNLGQAGFV 278
Query: 76 YAPANAQR------TPNPCLVSPLVSSPAAIGYDDDSKE--LFSMGRIDSHWLNRTTTKG 127
+ AN ++ T + P S +I + +K+ + GR SH + T G
Sbjct: 279 FIEANVKKWGLAPVTGSLAPPPPYGSEQGSILLEAGTKDGSTYPQGRQHSH----SITAG 334
Query: 128 SHNNGN 133
S+N N
Sbjct: 335 SYNTRN 340
>emb|CAG84154.1| unnamed protein product [Yarrowia lipolytica CLIB99]
gi|50545367|ref|XP_500221.1| hypothetical protein
[Yarrowia lipolytica]
Length = 468
Score = 41.2 bits (95), Expect = 0.010
Identities = 24/67 (35%), Positives = 36/67 (52%), Gaps = 5/67 (7%)
Query: 18 VIGCGFDPRQKKVFFTLDSE-LMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKY 76
V+G G+ PR +FFT + + L VVH L+PT+ A + VNFGQ+ F +
Sbjct: 245 VVGVGYRPRSGTIFFTRNGKRLEEVVH----GMRLNLFPTVGATGPCQLHVNFGQSGFVF 300
Query: 77 APANAQR 83
AN ++
Sbjct: 301 IEANVKK 307
>gb|EAK86445.1| hypothetical protein UM05579.1 [Ustilago maydis 521]
gi|49078998|ref|XP_403194.1| hypothetical protein
UM05579.1 [Ustilago maydis 521]
Length = 799
Score = 41.2 bits (95), Expect = 0.010
Identities = 26/85 (30%), Positives = 40/85 (46%), Gaps = 2/85 (2%)
Query: 14 GTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNA 73
G VIG G+ PR VFFT + + + ++ L+PT+ A+ + VN GQ
Sbjct: 383 GQGDVIGIGYRPRTGTVFFTRNGKRLEDAFIGLNRYN--LFPTVGADGACEIHVNLGQAG 440
Query: 74 FKYAPANAQRTPNPCLVSPLVSSPA 98
F + AN ++ +V L PA
Sbjct: 441 FVFIEANVKKWGLAPMVGTLAPPPA 465
>gb|EAA55403.1| hypothetical protein MG09210.4 [Magnaporthe grisea 70-15]
gi|39957891|ref|XP_364365.1| hypothetical protein
MG09210.4 [Magnaporthe grisea 70-15]
Length = 504
Score = 40.8 bits (94), Expect = 0.013
Identities = 24/68 (35%), Positives = 38/68 (55%), Gaps = 6/68 (8%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVH--CQTEEFSTPLYPTLAANIDIMVLVNFGQNAFK 75
VIG G+ PR +FFT + + + V +T+ F +P++ AN +V VNFGQ F
Sbjct: 219 VIGVGYRPRTGAIFFTRNGKRLEDVAQGLKTQNF----FPSIGANGPCIVHVNFGQAGFV 274
Query: 76 YAPANAQR 83
+ AN ++
Sbjct: 275 FIEANVKK 282
>gb|EAL18533.1| hypothetical protein CNBJ1750 [Cryptococcus neoformans var.
neoformans B-3501A] gi|57229482|gb|AAW45915.1| endosome
protein, putative [Cryptococcus neoformans var.
neoformans JEC21] gi|58260044|ref|XP_567432.1| endosome
protein, putative [Cryptococcus neoformans var.
neoformans JEC21]
Length = 665
Score = 40.0 bits (92), Expect = 0.023
Identities = 24/81 (29%), Positives = 38/81 (46%), Gaps = 2/81 (2%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKYA 77
V+G G+ PR VFFT + + + + + L+PT+ AN + VN GQ F +
Sbjct: 297 VLGVGYRPRTGTVFFTRNGRKLEDAYTGLQRLN--LFPTVGANGPCTLHVNLGQAGFVFI 354
Query: 78 PANAQRTPNPCLVSPLVSSPA 98
AN ++ + L PA
Sbjct: 355 EANVKKWGLAPMTGTLAPPPA 375
>gb|AAS51641.1| ADL279Wp [Ashbya gossypii ATCC 10895] gi|45187594|ref|NP_983817.1|
ADL279Wp [Eremothecium gossypii]
Length = 493
Score = 39.7 bits (91), Expect = 0.030
Identities = 29/92 (31%), Positives = 42/92 (45%), Gaps = 5/92 (5%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVV----HCQTEEFSTPLYPTLAANIDIMVLVNFGQNA 73
VIG G+ R VFFT + + ++ H + + LYP + AN+ + VNFG
Sbjct: 295 VIGIGYRTRSGTVFFTRNGKKLNEKRIGGHIKGWKIKY-LYPIVGANVPCKIHVNFGTYG 353
Query: 74 FKYAPANAQRTPNPCLVSPLVSSPAAIGYDDD 105
F Y AN ++ L + PA YD D
Sbjct: 354 FVYIEANVKKWGYAKLNGMKLPPPAYEDYDRD 385
>ref|XP_650388.1| SPRY domain protein [Entamoeba histolytica HM-1:IMSS]
gi|56467009|gb|EAL45002.1| SPRY domain protein
[Entamoeba histolytica HM-1:IMSS]
Length = 369
Score = 39.7 bits (91), Expect = 0.030
Identities = 43/168 (25%), Positives = 70/168 (41%), Gaps = 24/168 (14%)
Query: 2 KLVFESEK----AEWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTL 57
K+ + S K A+ G +GCGFD VFFT++ + ++ + F YP +
Sbjct: 190 KMFYNSPKGKAFAKPYGQGDTVGCGFDVSNHIVFFTINGVSLPLIPVKENAF----YPAI 245
Query: 58 AANIDIMVLVNFGQNAFKYAPANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGRIDS 117
+ +NFG+ FKY + + V ++ D S LFS ++ +
Sbjct: 246 SLRSYKDFTINFGEEPFKYDVYHFKTKDE---VKDGHKDQSSEQIFDKSNLLFS-SKMLN 301
Query: 118 HWLNRTTTKGSHNNGNNIST----MDFDEESEADLFEIVIDGSRKSPS 161
H+LN N+ N ++ M+ DE SE DGS + S
Sbjct: 302 HFLNSEDESSGSNSFNPTNSKCERMNKDEISE--------DGSNRDSS 341
>gb|AAX70633.1| hypothetical protein, conserved [Trypanosoma brucei]
Length = 6613
Score = 39.3 bits (90), Expect = 0.039
Identities = 27/86 (31%), Positives = 45/86 (51%), Gaps = 7/86 (8%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANID---IMVLVNFGQNA- 73
VIGCG +FFT + E + + TE + + P +A D + ++VNFG+NA
Sbjct: 1936 VIGCGIMAPSSSLFFTRNGEFLGIA---TECTFSTIIPFVAVQTDGMLVKLIVNFGENAP 1992
Query: 74 FKYAPANAQRTPNPCLVSPLVSSPAA 99
F++ A+ + VSP++ S A+
Sbjct: 1993 FEFDMASLHSSCRTQTVSPVMISDAS 2018
>emb|CAG59231.1| unnamed protein product [Candida glabrata CBS138]
gi|50287755|ref|XP_446307.1| unnamed protein product
[Candida glabrata]
Length = 580
Score = 39.3 bits (90), Expect = 0.039
Identities = 38/138 (27%), Positives = 58/138 (41%), Gaps = 31/138 (22%)
Query: 18 VIGCGFDPRQKKVFFTLD----SELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNA 73
+IG G+ R VFFT + SE H + +F LYP + AN+ + VNFG
Sbjct: 329 IIGIGYRTRSGTVFFTRNGKKISEKSVGGHIKGWKFKY-LYPVVGANVPCKIHVNFGSYG 387
Query: 74 FKYAPANAQR----TPNPCLVSPLVSSPAAIGYDDDSKELFSMGRIDSHWLNRTTTKGSH 129
F + AN ++ PN + P YDD ++ T + +
Sbjct: 388 FVFIEANVKKWGYAKPNGIKLPP-------PSYDD--------------YVQDTLLESGY 426
Query: 130 NNGNNISTMDFDEESEAD 147
+ +N S D D+ES+ D
Sbjct: 427 EDNDN-SDNDSDDESDTD 443
>ref|XP_637470.1| hypothetical protein DDB0187197 [Dictyostelium discoideum]
gi|60465893|gb|EAL63963.1| hypothetical protein
DDB0187197 [Dictyostelium discoideum]
Length = 5222
Score = 38.9 bits (89), Expect = 0.051
Identities = 21/59 (35%), Positives = 31/59 (51%), Gaps = 3/59 (5%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFGQNAFKY 76
+IGCGF+ + KVFFT + + + + YP +A + V VNFGQ F+Y
Sbjct: 2704 IIGCGFNHKDGKVFFTKNGKYLGTAF---KNVIGDFYPVVAMSSGGSVGVNFGQQPFQY 2759
>emb|CAA89807.1| unknown [Saccharomyces cerevisiae]
Length = 336
Score = 37.7 bits (86), Expect = 0.11
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVV----HCQTEEFSTPLYPTLAANIDIMVLVNFGQNA 73
++G G+ R VFFT + + ++ H + +F LYP + +N+ + VNFG
Sbjct: 102 IVGIGYRSRSGTVFFTRNGKKLNEKSVGGHIRGWKFQY-LYPIIGSNVPCQIHVNFGTYG 160
Query: 74 FKYAPANAQR 83
F Y AN ++
Sbjct: 161 FVYIEANVKK 170
>ref|NP_013894.1| Endosomal protein of unknown function, mRNA is targeted to the bud
via the mRNA transport system involving She2p; Ymr171cp
[Saccharomyces cerevisiae] gi|2132725|pir||S55118
probable membrane protein YMR171c - yeast (Saccharomyces
cerevisiae) gi|2497171|sp|Q03212|YM40_YEAST Hypothetical
62.5 kDa protein in ALD2-DDR48 intergenic region
Length = 550
Score = 37.7 bits (86), Expect = 0.11
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Query: 18 VIGCGFDPRQKKVFFTLDSELMHVV----HCQTEEFSTPLYPTLAANIDIMVLVNFGQNA 73
++G G+ R VFFT + + ++ H + +F LYP + +N+ + VNFG
Sbjct: 316 IVGIGYRSRSGTVFFTRNGKKLNEKSVGGHIRGWKFQY-LYPIIGSNVPCQIHVNFGTYG 374
Query: 74 FKYAPANAQR 83
F Y AN ++
Sbjct: 375 FVYIEANVKK 384
>gb|EAL30393.1| GA21506-PA [Drosophila pseudoobscura]
Length = 723
Score = 37.4 bits (85), Expect = 0.15
Identities = 28/92 (30%), Positives = 41/92 (44%), Gaps = 6/92 (6%)
Query: 11 EWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNFG 70
E G VIGC D ++ ++ FT + + + V E S + + +L NFG
Sbjct: 179 EAFGKADVIGCLLDLQRLEMSFTKNGQSLGVAFRVPENLSKETFYPAVVLKNAEMLFNFG 238
Query: 71 QNAFKYAPAN-----AQRTPNPCLVSPLVSSP 97
+ FKY+P N Q P +PL SSP
Sbjct: 239 KTDFKYSPGNGFVGVCQAGPEHSKTNPL-SSP 269
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.316 0.132 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 292,487,606
Number of Sequences: 2540612
Number of extensions: 12144022
Number of successful extensions: 31058
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 38
Number of HSP's that attempted gapping in prelim test: 31014
Number of HSP's gapped (non-prelim): 59
length of query: 164
length of database: 863,360,394
effective HSP length: 118
effective length of query: 46
effective length of database: 563,568,178
effective search space: 25924136188
effective search space used: 25924136188
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 70 (31.6 bits)
Medicago: description of AC122728.13