BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149600.13 + phase: 0 /pseudo
(232 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g08620 unknown protein 297 4e-81
At2g38610 putative RNA-binding protein 291 2e-79
At5g56140 RNA-binding protein-like 231 2e-61
At4g26480 putative protein 228 2e-60
At1g09660 elongation factor like protein 224 3e-59
At5g51300 unknown protein 99 1e-21
At1g09670 unknown protein 37 0.009
At3g32940 hypothetical protein 34 0.075
At4g30830 unknown protein 30 1.4
At1g76990 unknown protein 30 1.4
At5g42750 unknown protein 29 1.9
At5g08420 rev interacting protein mis3 - like 28 3.2
At2g28140 unknown protein 28 3.2
At1g17320 unknown protein, 3' partial 28 3.2
At4g11790 unknown protein 28 5.4
At5g55820 unknown protein 27 7.0
At5g42270 cell division protein FtsH 27 7.0
At4g23590 tyrosine transaminase like protein 27 7.0
At3g02920 replication factor A like protein 27 7.0
At5g35200 unknown protein 27 9.2
>At3g08620 unknown protein
Length = 283
Score = 297 bits (760), Expect = 4e-81
Identities = 155/240 (64%), Positives = 189/240 (78%), Gaps = 13/240 (5%)
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSS---FTG 57
MQVLP+C+RLLNQEI R +G +M NQGF+D+DR++ S P P+L + S G
Sbjct: 46 MQVLPICSRLLNQEIFRITG---MMPNQGFTDFDRLRHRSPSPMASPNLMSNVSGGGLGG 102
Query: 58 WNSLSHE------GLNVDWQRAPAISNSHIVKKMLRLDIPHDNHPTFNFVGRLLGPRGNS 111
WN L E G+ ++WQ APA +S+ VK++LRLD+P D +P FNFVGRLLGPRGNS
Sbjct: 103 WNGLPPERIGGPHGMAMEWQGAPASPSSYPVKRILRLDLPVDTYPNFNFVGRLLGPRGNS 162
Query: 112 LKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVDLRLR 171
LKRVEATTGCRV+IRGKGSIKD +KEE L+G+PG+EHLNE LHILIEA+LP+++VD++LR
Sbjct: 163 LKRVEATTGCRVYIRGKGSIKDPEKEEKLKGKPGYEHLNEQLHILIEADLPIDIVDIKLR 222
Query: 172 QAQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREESPQLSGSLSPFTSNEMIKRAKT 231
QAQEIIEEL+KPVDESQD KRQQLRELA+LNS+ RE SP SGS+SPF SN M KR KT
Sbjct: 223 QAQEIIEELVKPVDESQDYIKRQQLRELALLNSNLRENSPGPSGSVSPFNSNAM-KRPKT 281
>At2g38610 putative RNA-binding protein
Length = 286
Score = 291 bits (746), Expect = 2e-79
Identities = 159/243 (65%), Positives = 188/243 (76%), Gaps = 17/243 (6%)
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSSFTG--- 57
MQVLP+C+RLLNQE+ R SG +M NQGF D+DR++ S P +L + S TG
Sbjct: 47 MQVLPICSRLLNQEMFRVSG---MMSNQGFGDFDRLRHRSPSPMASSNLMSNVSNTGLGG 103
Query: 58 WNSLSHE------GLNVDWQRAPAISNSHIVKKMLRLDIPHDNHPTFNFVGRLLGPRGNS 111
WN LS E G+ +DWQ AP +S+ VK++LRL+IP DN+P FNFVGRLLGPRGNS
Sbjct: 104 WNGLSQERLSGTPGMTMDWQGAPGSPSSYTVKRILRLEIPVDNYPNFNFVGRLLGPRGNS 163
Query: 112 LKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVDLRLR 171
LKRVEATTGCRVFIRGKGSIKD +KE+ LRGRPG+EHLNE LHILIEA+LP ++V++RLR
Sbjct: 164 LKRVEATTGCRVFIRGKGSIKDPEKEDKLRGRPGYEHLNEQLHILIEADLPASIVEIRLR 223
Query: 172 QAQEIIEELLKPVDESQDIYKRQQLRELAMLNS-SFREESPQLS--GSLSPFTSNEMIKR 228
QAQEIIEELLKPVDESQD KRQQLRELA+LNS + REESP S GS+SPF S+ KR
Sbjct: 224 QAQEIIEELLKPVDESQDFIKRQQLRELALLNSNNLREESPGPSGGGSVSPFNSSG--KR 281
Query: 229 AKT 231
KT
Sbjct: 282 PKT 284
>At5g56140 RNA-binding protein-like
Length = 315
Score = 231 bits (589), Expect = 2e-61
Identities = 128/241 (53%), Positives = 166/241 (68%), Gaps = 18/241 (7%)
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPS----LDTTSSFT 56
+ VLP RLLNQEILR + L++N Q G PS + S + +
Sbjct: 80 LPVLPHAFRLLNQEILRVTT---LLENATVLS----QSGLDHPSPLASGGIFQNARADMN 132
Query: 57 GWNSLSHEGLNV------DWQRAPAISNSHIVKKMLRLDIPHDNHPTFNFVGRLLGPRGN 110
GW S +V +W +P S+ I K+ +R+DIP DN+P FNFVGRLLGPRGN
Sbjct: 133 GWASQFPSERSVPSSPGPNWLNSPGSSSGLIAKRTIRVDIPVDNYPNFNFVGRLLGPRGN 192
Query: 111 SLKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVDLRL 170
SLKRVEA+T CRV IRG+GSIKD KEE++RG+PG+EHLNEPLHIL+EAELP+ +VD RL
Sbjct: 193 SLKRVEASTDCRVLIRGRGSIKDPIKEEMMRGKPGYEHLNEPLHILVEAELPIEIVDARL 252
Query: 171 RQAQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREESPQLSGSLSPFTSNEMIKRAK 230
QA+EI+++LL P++E+ D+YK+QQLRELA+LN + REE +SGS+SP+ S M KRAK
Sbjct: 253 MQAREILDDLLTPMEETHDMYKKQQLRELALLNGTLREEGSPMSGSVSPYNSLGM-KRAK 311
Query: 231 T 231
T
Sbjct: 312 T 312
>At4g26480 putative protein
Length = 555
Score = 228 bits (582), Expect = 2e-60
Identities = 124/239 (51%), Positives = 165/239 (68%), Gaps = 16/239 (6%)
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSL--DTTSSFTGW 58
+ VLP RL+NQEILR + L++N + +F P + ++ + GW
Sbjct: 323 LPVLPHVCRLMNQEILRVTT---LLENA----LSQSRFDHPSPLASGGIFQNSRADMNGW 375
Query: 59 NSLSHEGLNV------DWQRAPAISNSHIVKKMLRLDIPHDNHPTFNFVGRLLGPRGNSL 112
S +V +W +P S+ IVK+ +R+DIP D +P +NFVGRLLGPRGNSL
Sbjct: 376 ASQFPSERSVSSSPAPNWLNSPGSSSGLIVKRTIRVDIPVDKYPNYNFVGRLLGPRGNSL 435
Query: 113 KRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVDLRLRQ 172
KRVEA+T CRV IRG+GSIKD KE+++RG+PG+EHLNEPLHIL+EAELP+ +VD RL Q
Sbjct: 436 KRVEASTDCRVLIRGRGSIKDPIKEDMMRGKPGYEHLNEPLHILVEAELPIEIVDARLMQ 495
Query: 173 AQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREESPQLSGSLSPFTSNEMIKRAKT 231
A+EI+++LL PV+E+ D YK+QQLRELA+LN S REE +SGS+SP+ S M KRAKT
Sbjct: 496 AREILDDLLTPVEETHDFYKKQQLRELALLNGSLREEGSPMSGSISPYNSLGM-KRAKT 553
>At1g09660 elongation factor like protein
Length = 298
Score = 224 bits (571), Expect = 3e-59
Identities = 126/251 (50%), Positives = 157/251 (62%), Gaps = 32/251 (12%)
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSSFTGWNS 60
+QV+P C RLLN EI R S F D DR + GS SL + GW+
Sbjct: 60 LQVMPNCCRLLNHEIRRVSS---------FPDLDRYEHGSPFRSLGQPTNGKLDLEGWSM 110
Query: 61 LSHEG-------------LNVDWQRAPAISNSHIVKKMLRLDIPHDNHPTFNFVGRLLGP 107
+ E V W P + N IVKK++RLD+P D +P++NFVGR+LGP
Sbjct: 111 MQAEENCHLQRASPFRGPSPVGWIGMPGLPNPPIVKKVIRLDVPVDKYPSYNFVGRILGP 170
Query: 108 RGNSLKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVD 167
RGNSLKRVE T CRVFIRG+GS+KD KEE L+G+PG+EHL EPLH+LIEAELP ++++
Sbjct: 171 RGNSLKRVELATHCRVFIRGRGSVKDTVKEEKLKGKPGYEHLCEPLHVLIEAELPEDIIN 230
Query: 168 LRLRQAQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREESPQ------LSGSLSPFT 221
RL A +E LLKP+DES D YKR+QL+ELA LN + REESP LS S+SPF
Sbjct: 231 SRLEHAVHFLESLLKPMDESMDHYKREQLKELAALNGTLREESPSPSLSPCLSPSMSPFN 290
Query: 222 SNEMIKRAKTD 232
S KRAKT+
Sbjct: 291 S----KRAKTE 297
>At5g51300 unknown protein
Length = 804
Score = 99.4 bits (246), Expect = 1e-21
Identities = 56/125 (44%), Positives = 81/125 (64%), Gaps = 10/125 (8%)
Query: 87 RLDIPHDNHPTFNFVGRLLGPRGNSLKRVEATTGCRVFIRGKGSIKD--FDKEELLRGRP 144
+L IP P +NF+G ++GPRGN+ KR+E TG ++ IRGKGS+K+ +++ L+ P
Sbjct: 244 KLFIPMKEFPGYNFIGLIIGPRGNTQKRMERETGAKIVIRGKGSVKEGRHQQKKDLKYDP 303
Query: 145 GFEHLNEPLHILIEAELPVNVVDLRLRQAQEIIEELLKPVDESQDIYKRQQLRELAMLNS 204
NE LH+L+EAE L A ++E+LL+PVDE + +KRQQLRELA LN
Sbjct: 304 S---ENEDLHVLVEAE-----TQEALEAAAGMVEKLLQPVDEVLNEHKRQQLRELATLNG 355
Query: 205 SFREE 209
+ R+E
Sbjct: 356 TIRDE 360
>At1g09670 unknown protein
Length = 118
Score = 37.0 bits (84), Expect = 0.009
Identities = 23/64 (35%), Positives = 29/64 (44%), Gaps = 9/64 (14%)
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSSFTGWNS 60
+QV+P C RLLN EI R S F D DR + GS SL + GW+
Sbjct: 60 LQVMPNCCRLLNHEIRRVS---------SFPDLDRYEHGSPFRSLGQPTNGKLDLEGWSM 110
Query: 61 LSHE 64
+ E
Sbjct: 111 MQAE 114
>At3g32940 hypothetical protein
Length = 590
Score = 33.9 bits (76), Expect = 0.075
Identities = 17/44 (38%), Positives = 25/44 (56%)
Query: 84 KMLRLDIPHDNHPTFNFVGRLLGPRGNSLKRVEATTGCRVFIRG 127
K RL I H F+F+ + G +G++ KR+E TG +V I G
Sbjct: 116 KEARLPIDVKEHSDFSFLSLIFGSQGDTQKRLEKETGAKVQIFG 159
>At4g30830 unknown protein
Length = 363
Score = 29.6 bits (65), Expect = 1.4
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 6/54 (11%)
Query: 162 PVNVVDLRLRQAQEIIEELLKPVDESQDIYKRQ------QLRELAMLNSSFREE 209
P V DL + + +E+IE+ L+ S D+Y Q Q++EL S R++
Sbjct: 156 PELVTDLSIPEEKEVIEQSLESQKSSLDVYWEQIKKLNEQVKELTGFRDSMRDQ 209
>At1g76990 unknown protein
Length = 453
Score = 29.6 bits (65), Expect = 1.4
Identities = 22/70 (31%), Positives = 36/70 (51%), Gaps = 6/70 (8%)
Query: 97 TFNFVGRLLGPRGNSLKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHIL 156
T +++ ++LGP+G++ G RV + G E + R RPG L+E +L
Sbjct: 94 TIDYIEKVLGPKGHASASQNTWPGKRVGVHSLGDHTSI--EIIARDRPGL--LSEVSAVL 149
Query: 157 IEAELPVNVV 166
A+L +NVV
Sbjct: 150 --ADLNINVV 157
>At5g42750 unknown protein
Length = 337
Score = 29.3 bits (64), Expect = 1.9
Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 10/60 (16%)
Query: 79 SHIVKKMLRLDIPHDNHPT----------FNFVGRLLGPRGNSLKRVEATTGCRVFIRGK 128
SH VKK +R+ + T ++F L+GP GNS + + R IRG+
Sbjct: 205 SHAVKKYIRMLFQKRGNGTQFWNRRQTSSYSFSSSLMGPNGNSKTMINGSYNKRDLIRGR 264
>At5g08420 rev interacting protein mis3 - like
Length = 391
Score = 28.5 bits (62), Expect = 3.2
Identities = 10/25 (40%), Positives = 17/25 (68%)
Query: 103 RLLGPRGNSLKRVEATTGCRVFIRG 127
RL+GP ++LK +E T C + ++G
Sbjct: 162 RLVGPNSSTLKALEILTNCYILVQG 186
>At2g28140 unknown protein
Length = 211
Score = 28.5 bits (62), Expect = 3.2
Identities = 14/50 (28%), Positives = 31/50 (62%), Gaps = 1/50 (2%)
Query: 167 DLRLRQAQEIIEELLKPVDESQDIYKRQQLRELAML-NSSFREESPQLSG 215
D ++ Q ++++ + +K DE+Q+ YKR L + +L + + E+ P ++G
Sbjct: 52 DDQIMQLKDLLNKAIKEKDEAQERYKRILLDQNFLLQHQTDEEQDPNING 101
>At1g17320 unknown protein, 3' partial
Length = 83
Score = 28.5 bits (62), Expect = 3.2
Identities = 15/40 (37%), Positives = 27/40 (67%), Gaps = 2/40 (5%)
Query: 170 LRQAQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREE 209
+R+A+E++E+ +K D S D + ++R+LA+ S REE
Sbjct: 6 MRKAEELVEKAMKGNDASHDAWHVWRVRDLAL--SIAREE 43
>At4g11790 unknown protein
Length = 443
Score = 27.7 bits (60), Expect = 5.4
Identities = 21/61 (34%), Positives = 30/61 (48%), Gaps = 8/61 (13%)
Query: 18 ASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDT-------TSSFTGWNSLSHEGLNVDW 70
+S SGL+ N + QFG+TK S PSL + ++S G S SH G+ +
Sbjct: 179 SSSPSGLISNSQTGSFSSGQFGTTKSS-QPSLFSGSQAGAISNSQPGTFSSSHSGITSNS 237
Query: 71 Q 71
Q
Sbjct: 238 Q 238
>At5g55820 unknown protein
Length = 1826
Score = 27.3 bits (59), Expect = 7.0
Identities = 17/47 (36%), Positives = 25/47 (53%)
Query: 164 NVVDLRLRQAQEIIEELLKPVDESQDIYKRQQLRELAMLNSSFREES 210
+V D+ + Q+ E I+E VDE D K + E A L+ S R +S
Sbjct: 402 HVNDIDILQSIETIDEASGKVDEQVDDPKSRSCYETAYLDGSTRSKS 448
>At5g42270 cell division protein FtsH
Length = 704
Score = 27.3 bits (59), Expect = 7.0
Identities = 24/104 (23%), Positives = 44/104 (42%), Gaps = 15/104 (14%)
Query: 122 RVFIRGKGSIKDFDKEELLRGRPGF------EHLNEPLHILIEAELPVNVVDLRLRQAQE 175
+V RGK KD D E++ R PGF +NE + EL ++ + +
Sbjct: 430 KVHSRGKAIGKDVDYEKVARRTPGFTGADLQNLMNEAAILAARRELK----EISKDEISD 485
Query: 176 IIEELLKPVDESQDIYKRQQLRELAMLNSSFREESPQLSGSLSP 219
+E ++ ++ + ++ R +A + E L G+L P
Sbjct: 486 ALERIIAGPEKKNAVVSEEKKRLVA-----YHEAGHALVGALMP 524
>At4g23590 tyrosine transaminase like protein
Length = 424
Score = 27.3 bits (59), Expect = 7.0
Identities = 23/99 (23%), Positives = 42/99 (42%), Gaps = 11/99 (11%)
Query: 115 VEATTGCRVFIRGKGSIKDFDKEELLRGRPGF-----EHLNEPLHILIEAELPVNVVDLR 169
V T GC+ I SI K +L RPGF + + L + +P ++
Sbjct: 103 VYMTVGCKQAIELAVSILAKPKANILLPRPGFPWDMVHSIYKHLEVRRYEFIPERDFEID 162
Query: 170 LRQAQEIIEE------LLKPVDESQDIYKRQQLRELAML 202
+E+++E ++ P + + + Y L++LA L
Sbjct: 163 FNSVREMVDENTFAIFIINPHNPNGNYYTEAHLKQLATL 201
>At3g02920 replication factor A like protein
Length = 278
Score = 27.3 bits (59), Expect = 7.0
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 4/37 (10%)
Query: 148 HLNEPLHILIEAELPVNVVDLRLR----QAQEIIEEL 180
+LN+P+HI+ EA + +++ LR Q +E +E+L
Sbjct: 221 YLNQPMHIVSEAGVHCDIIARELRIPLLQVKEALEQL 257
>At5g35200 unknown protein
Length = 544
Score = 26.9 bits (58), Expect = 9.2
Identities = 16/45 (35%), Positives = 24/45 (52%), Gaps = 1/45 (2%)
Query: 147 EHLNEPLHIL-IEAELPVNVVDLRLRQAQEIIEELLKPVDESQDI 190
E L P IL IE E+P VV+ + + + E KPV++ D+
Sbjct: 316 EKLTAPKEILAIEYEIPPKVVEEKPASPEPVKAEAEKPVEKQPDL 360
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,252,862
Number of Sequences: 26719
Number of extensions: 230965
Number of successful extensions: 657
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 631
Number of HSP's gapped (non-prelim): 25
length of query: 232
length of database: 11,318,596
effective HSP length: 96
effective length of query: 136
effective length of database: 8,753,572
effective search space: 1190485792
effective search space used: 1190485792
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC149600.13