BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149582.7 - phase: 0
(155 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g76070 unknown protein 63 6e-11
At2g37100 unknown protein 50 6e-07
At1g20310 unknown protein 49 8e-07
At5g03110 putative protein 42 1e-04
At2g36220 unknown protein 39 0.001
At3g52710 unknown protein 38 0.002
At1g36940 hypothetical protein 37 0.003
At3g15095 unknown protein, 3' partial 36 0.009
At1g31660 putative bystin 36 0.009
At4g38410 cold-regulated protein - like 35 0.012
At1g22230 hypothetical protein 34 0.027
At3g44270 putative protein 34 0.036
At3g57800 bHLH like transcription factor (bHLH060) 33 0.047
At3g28770 hypothetical protein 32 0.18
At5g14440 putative protein 31 0.23
At3g42660 putative protein 31 0.30
At5g38720 unknown protein 30 0.40
At1g48490 IRE - like protein kinase 30 0.40
At1g09600 putative protein kinase 30 0.40
At3g19060 hypothetical protein 30 0.52
>At1g76070 unknown protein
Length = 272
Score = 63.2 bits (152), Expect = 6e-11
Identities = 56/187 (29%), Positives = 75/187 (39%), Gaps = 46/187 (24%)
Query: 6 KMKNKFFKFLPKRPVASVSSYQNPTLSPNASV--------TTARK-------VSIIPKEA 50
K KNK K LPK A ++ P SP + T A K V ++P A
Sbjct: 13 KNKNKLLKMLPK---AMSFGHRVPPFSPGRDLHHNNHHNYTAANKMFFSGPMVPLVPNAA 69
Query: 51 RRKHRSISFSAREPSSPKVSCMGQVK--------SKKKKRKAKRIHQSSTKNNDSVTSHE 102
R + EP+SPKVSC+GQ+K KK K + I + S + S+T +
Sbjct: 70 RVRRNKSDAVWDEPTSPKVSCIGQIKLGKSKCPTGKKNKAPSSLIPKISKTSTSSLTKED 129
Query: 103 NKKILLQSEKAL--------------------VVEENQEFAPSMLIVPSLGTMKKFESGR 142
K L + + +E+ S VPSLG MKKF S R
Sbjct: 130 EKGRLSKIKSIFSFSPASGRNTSRKSHPTAVSAADEHPVTVVSTAAVPSLGQMKKFASSR 189
Query: 143 GSLSDFD 149
+L DFD
Sbjct: 190 DALGDFD 196
>At2g37100 unknown protein
Length = 297
Score = 49.7 bits (117), Expect = 6e-07
Identities = 33/88 (37%), Positives = 48/88 (54%), Gaps = 7/88 (7%)
Query: 13 KFLPKRPVASVSSYQNPTLSPN----ASVTTARKVSIIPKEARRKHRSISFSAREPSSPK 68
+F RPV+S +NP L S + +R S P RRK+ S + +EP+SPK
Sbjct: 2 RFSSSRPVSSPGRTENPPLLMRFLRTKSRSRSRSRSRRPIFFRRKNASAAAETQEPTSPK 61
Query: 69 VSCMGQV---KSKKKKRKAKRIHQSSTK 93
V+CMGQV +SKK K + R+ +T+
Sbjct: 62 VTCMGQVRINRSKKPKPETARVSGGATE 89
>At1g20310 unknown protein
Length = 240
Score = 49.3 bits (116), Expect = 8e-07
Identities = 53/188 (28%), Positives = 74/188 (39%), Gaps = 37/188 (19%)
Query: 1 MEKLSKMKNKFFKFLPKRPVASVSSYQNPTLSPNASVTTARKVS-----------IIPKE 49
MEK S NK KF A + SP R S ++P
Sbjct: 1 MEKASN-SNKSTKFSKMLQRAMSIGHSAAPFSPRRDFHQHRTTSTANRGIFFSSPLVPTA 59
Query: 50 AR-RKHRSISFSAREPSSPKVSCMGQVK------SKKKKRKAKRIHQSST------KNND 96
AR R++ EP+SPKVSC+GQVK +KK + K + +S+ K D
Sbjct: 60 ARVRRNTKYEAVFAEPTSPKVSCIGQVKLARPKCPEKKNKAPKNLKTASSLSSCVIKEED 119
Query: 97 SVTSHENKKILLQSEKALVVEENQEFA------------PSMLIVPSLGTMKKFESGRGS 144
+ + + K+I + FA ++ PSLG MKKF S R +
Sbjct: 120 NGSFSKLKRIFSMRSYPSRKSNSTAFAAAAAREHPIAEVDAVTAAPSLGAMKKFASSREA 179
Query: 145 LSDFDATL 152
L FD T+
Sbjct: 180 LGGFDWTV 187
>At5g03110 putative protein
Length = 283
Score = 42.0 bits (97), Expect = 1e-04
Identities = 30/84 (35%), Positives = 46/84 (54%), Gaps = 13/84 (15%)
Query: 15 LPKRPVAS---VSSYQNPTL----SPNASVTTARKVSIIPKEAR------RKHRSISFSA 61
+ +RPV+S V Y P + S + +T+R S +R R+++S + A
Sbjct: 3 ISRRPVSSPGRVEKYPPPFMGFLRSKSNGGSTSRSRSRSRGRSRASPLFVRRNKSAAAVA 62
Query: 62 REPSSPKVSCMGQVKSKKKKRKAK 85
+EPSSPKV+CMGQV+ + K K K
Sbjct: 63 QEPSSPKVTCMGQVRVNRSKPKIK 86
>At2g36220 unknown protein
Length = 263
Score = 38.9 bits (89), Expect = 0.001
Identities = 28/113 (24%), Positives = 48/113 (41%), Gaps = 32/113 (28%)
Query: 60 SAREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKIL------------ 107
S EPSSPKVSC+G+V+S+++ +++R+H+ + + V + +
Sbjct: 143 SLLEPSSPKVSCIGRVRSRRE--RSRRMHRQKSSRVEPVNRVKKPGFMASFRAIFRIKGG 200
Query: 108 ------------------LQSEKALVVEENQEFAPSMLIVPSLGTMKKFESGR 142
++S +E F +VP LG M +F SGR
Sbjct: 201 CKDVSARETHTSTRNTHDIRSRLPAEADEKSVFDGGEPVVPGLGGMTRFASGR 253
>At3g52710 unknown protein
Length = 289
Score = 38.1 bits (87), Expect = 0.002
Identities = 33/116 (28%), Positives = 54/116 (46%), Gaps = 29/116 (25%)
Query: 9 NKFFKFLPKRPVASVSSYQNPTLSPNAS---VTTARKVSII--PK-------EARRKH-- 54
N FF+ + S + NP +PN+S ++ K SII PK EA+++
Sbjct: 64 NAFFERKESQKGNSSAPISNPNTNPNSSSHRISLKSKASIIRLPKPQKTCFNEAKKRRNC 123
Query: 55 ---RSISFSAR------------EPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNN 95
R++ R EP SPKVSC+G+V+SK+ + + + +S N+
Sbjct: 124 RIARTLMIPKRIGSRLKSDPTLSEPCSPKVSCIGRVRSKRDRSRRMQRQKSGRTNS 179
>At1g36940 hypothetical protein
Length = 181
Score = 37.4 bits (85), Expect = 0.003
Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Query: 26 YQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKK 80
+Q PT P + K +P ++ H+ S+ +P SPK+SC+GQVK K
Sbjct: 10 FQRPTFLPMLCSRPSIKNVTLPAKS---HQDDSYQQADPLSPKISCIGQVKRSNK 61
>At3g15095 unknown protein, 3' partial
Length = 417
Score = 35.8 bits (81), Expect = 0.009
Identities = 15/31 (48%), Positives = 22/31 (70%)
Query: 63 EPSSPKVSCMGQVKSKKKKRKAKRIHQSSTK 93
EPSSPKV+C+GQV+ K +K K++ S +
Sbjct: 109 EPSSPKVTCIGQVRVKTRKHVKKKMRARSRR 139
>At1g31660 putative bystin
Length = 444
Score = 35.8 bits (81), Expect = 0.009
Identities = 26/93 (27%), Positives = 37/93 (38%), Gaps = 6/93 (6%)
Query: 65 SSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEENQEFAP 124
+ P +S V S +K+ K + HQ K ++ S + K L +K + EEN E P
Sbjct: 11 TQPFISDDASVASSRKRSKVPKTHQKQEKLIEAGMSEKIMKQALAQQKEVADEENAERNP 70
Query: 125 SMLIVPSLGT------MKKFESGRGSLSDFDAT 151
S G K E + DFD T
Sbjct: 71 SSAAFAVAGAATAGEEQKILEEEEDDIDDFDGT 103
Score = 28.9 bits (63), Expect = 1.2
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 3/84 (3%)
Query: 29 PTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIH 88
P +S +ASV ++RK S +PK +++ + I E + + Q K + A+R
Sbjct: 13 PFISDDASVASSRKRSKVPKTHQKQEKLIEAGMSEKIMKQ--ALAQQKEVADEENAERNP 70
Query: 89 QSST-KNNDSVTSHENKKILLQSE 111
S+ + T+ E +KIL + E
Sbjct: 71 SSAAFAVAGAATAGEEQKILEEEE 94
>At4g38410 cold-regulated protein - like
Length = 163
Score = 35.4 bits (80), Expect = 0.012
Identities = 29/97 (29%), Positives = 43/97 (43%), Gaps = 14/97 (14%)
Query: 12 FKFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSC 71
F FL K+P ++ S NA VT K P A R H S S S+ E +
Sbjct: 24 FDFLKKKP-------EDVHSSENARVTKEPKEEEKPSLAERFHLSDSSSSDEEAGEN--- 73
Query: 72 MGQVKSKKKKRKAKRIHQSSTKNNDSVTS---HENKK 105
G+ K KKKK+K + + + + + + HE+ K
Sbjct: 74 -GEKKEKKKKKKKNEVAEDQCETEEKIPAGIGHEDGK 109
>At1g22230 hypothetical protein
Length = 314
Score = 34.3 bits (77), Expect = 0.027
Identities = 21/68 (30%), Positives = 32/68 (46%)
Query: 15 LPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQ 74
LP + ++S SS N + + K+S R+ EP+SPKV+C GQ
Sbjct: 28 LPSKSISSPSSSFNRRQNAPHHRRSISKLSSSGGGVRQNRGGGREVVEEPTSPKVTCAGQ 87
Query: 75 VKSKKKKR 82
+K + KR
Sbjct: 88 IKVRSSKR 95
>At3g44270 putative protein
Length = 324
Score = 33.9 bits (76), Expect = 0.036
Identities = 34/134 (25%), Positives = 56/134 (41%), Gaps = 14/134 (10%)
Query: 16 PKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQV 75
PK P + + +Q S ++S+ +K E SI++ + K S QV
Sbjct: 34 PKIPTQTEAEFQVEE-SVHSSIDRVQKTESTVPEVATSQPSIAYRLHKR---KQSTAAQV 89
Query: 76 KSKKKKRKAKRIHQSSTKNNDSVTSHE-NKKILLQSEKALVVEEN---------QEFAPS 125
++KK K K + SST S E K+ S K ++VEE +EF
Sbjct: 90 DKRQKKGKGKGVAASSTFEETRFVSREAEKRYAYFSHKNIIVEEGLSRKTDDKVREFIYD 149
Query: 126 MLIVPSLGTMKKFE 139
++ ++ +K FE
Sbjct: 150 AGLIRTVTELKPFE 163
>At3g57800 bHLH like transcription factor (bHLH060)
Length = 379
Score = 33.5 bits (75), Expect = 0.047
Identities = 29/83 (34%), Positives = 39/83 (46%), Gaps = 6/83 (7%)
Query: 25 SYQNPTLS-PNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRK 83
S + PT S P+ S +V P E R IS SA E P C Q K++
Sbjct: 122 SGETPTSSVPSNSSANLDRVKTEPAETDSSQRLISDSAIENQIP---CPNQNNRNGKRKD 178
Query: 84 AKRIHQSSTKNNDSVTSHENKKI 106
++ +SSTK N S S EN+K+
Sbjct: 179 FEKKGKSSTKKNKS--SEENEKL 199
>At3g28770 hypothetical protein
Length = 2081
Score = 31.6 bits (70), Expect = 0.18
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 10/70 (14%)
Query: 62 REPSSPKVSCMGQVKSKKKKRKAKRIHQSSTK----------NNDSVTSHENKKILLQSE 111
+E + ++ + K K KK+K K S+ K NN+ +NKK +SE
Sbjct: 926 KEENKDTINTSSKQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNELKKQEDNKKETTKSE 985
Query: 112 KALVVEENQE 121
+ + EEN++
Sbjct: 986 NSKLKEENKD 995
Score = 25.8 bits (55), Expect = 9.8
Identities = 15/74 (20%), Positives = 34/74 (45%)
Query: 48 KEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKIL 107
KE +++H +E + + KS+KK+ K + + +N++ +N+K
Sbjct: 1081 KEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKK 1140
Query: 108 LQSEKALVVEENQE 121
Q K + E +++
Sbjct: 1141 SQHVKLVKKESDKK 1154
>At5g14440 putative protein
Length = 291
Score = 31.2 bits (69), Expect = 0.23
Identities = 30/110 (27%), Positives = 47/110 (42%), Gaps = 8/110 (7%)
Query: 48 KEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKIL 107
+E R+ S S A +P + + KKKK+K + + K+ + + E+
Sbjct: 113 EEKEREKESGSIPAEGGETPAKENGVEDEDKKKKKKKNNKKKKNKKSVEKKKNGEDVADE 172
Query: 108 LQSEKALVVEENQEFAPSMLIVPSLGTMKKFESGR---GSLSDFDATLAE 154
++ E VEE EF +P G F+ GR GS SD D + E
Sbjct: 173 IEHENDEAVEEELEF-----WMPPDGERWDFDDGRDRWGSDSDSDVEMNE 217
>At3g42660 putative protein
Length = 951
Score = 30.8 bits (68), Expect = 0.30
Identities = 31/122 (25%), Positives = 57/122 (46%), Gaps = 5/122 (4%)
Query: 3 KLSKMKNKFFKFLPKRPV--ASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFS 60
KL + KF L +R + AS ++ NP L+PN V T + + A + + +
Sbjct: 808 KLPFLAEKFSSILEERLLEEASEAAVTNPALNPNGEVVTRVESKVQNPPASIQTSENTEA 867
Query: 61 AREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTK--NNDSVTSHENKKILLQSEKALVVEE 118
+ S+ K+S +K K K + ++ + TK +D E KK+ L++ V +E
Sbjct: 868 VMKSSATKLSAPTLLK-KSKVSEGLKLGKEQTKKDKSDDAKIKEIKKLNLKNPVNNVNKE 926
Query: 119 NQ 120
++
Sbjct: 927 DK 928
>At5g38720 unknown protein
Length = 306
Score = 30.4 bits (67), Expect = 0.40
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 10/84 (11%)
Query: 48 KEARRKHRSISFSAREPSSPKVSC------MGQVKSKKKKRKAKRIHQSST----KNNDS 97
K +RK + + EP +S G++ KKKRKA+ I +SS + +
Sbjct: 75 KMKKRKEKVGNVDISEPILEAISTEKVKEKKGKMNKTKKKRKAEEITRSSVEDLKRESKF 134
Query: 98 VTSHENKKILLQSEKALVVEENQE 121
S++ KK+ + S+K +EE ++
Sbjct: 135 KKSNKKKKMDMTSKKENKIEEEED 158
>At1g48490 IRE - like protein kinase
Length = 1235
Score = 30.4 bits (67), Expect = 0.40
Identities = 31/111 (27%), Positives = 52/111 (45%), Gaps = 5/111 (4%)
Query: 24 SSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRK 83
SS ++ + SP++S + S P + +K + S S E SP + S K K
Sbjct: 10 SSKKSRSSSPSSSNSPRSVGSDSPIRSDKK-KPKSNSKEETRSPNTK---KHVSAKGKEA 65
Query: 84 AKRIHQSST-KNNDSVTSHENKKILLQSEKALVVEENQEFAPSMLIVPSLG 133
+ +H SS K+N S + E KK + ++ V+E + S ++ SLG
Sbjct: 66 SLEVHSSSPGKSNSSSSGSEAKKPITETPATSDVKEESPASVSPIMASSLG 116
>At1g09600 putative protein kinase
Length = 714
Score = 30.4 bits (67), Expect = 0.40
Identities = 30/97 (30%), Positives = 46/97 (46%), Gaps = 11/97 (11%)
Query: 12 FKFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSS-PKVS 70
FK LP +A V + P+A TTA + + + S A +PSS PK
Sbjct: 413 FKSLPSSALALVEVLL--AVEPDARGTTASALE-------SEFFTTSPLASDPSSLPKYQ 463
Query: 71 CMGQVKSKKKKRKAKRIHQSSTKNNDS-VTSHENKKI 106
++ K ++ +AKR +S+K NDS S E+K +
Sbjct: 464 PRKEIDVKAQEEEAKRKKDTSSKQNDSKQVSRESKAV 500
>At3g19060 hypothetical protein
Length = 1647
Score = 30.0 bits (66), Expect = 0.52
Identities = 26/97 (26%), Positives = 48/97 (48%), Gaps = 20/97 (20%)
Query: 72 MGQVKSKKKKRKAKRIHQSSTKNN--------DSVTS-----HENKKILLQSEKA-LVVE 117
M +K +K++ KA S N+ ++V S +E+K L+ E A + +E
Sbjct: 606 MANLKGEKEELKASEKRSLSNLNDLAAQICNLNTVMSNMEEQYEHKMETLEHEIAKMKIE 665
Query: 118 ENQEFAPSMLIVPSLGTMKKFESGRGSLSDFDATLAE 154
+QE+ ++ I +KKFE +G++ + D T+ E
Sbjct: 666 ADQEYVENLCI------LKKFEEAQGTIREADITVNE 696
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.310 0.123 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,302,301
Number of Sequences: 26719
Number of extensions: 127911
Number of successful extensions: 657
Number of sequences better than 10.0: 99
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 67
Number of HSP's that attempted gapping in prelim test: 600
Number of HSP's gapped (non-prelim): 119
length of query: 155
length of database: 11,318,596
effective HSP length: 91
effective length of query: 64
effective length of database: 8,887,167
effective search space: 568778688
effective search space used: 568778688
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149582.7