BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149576.1 - phase: 1
(110 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45041 unknown protein 195 4e-51
At5g23670 serine palmitoyltransferase 28 0.75
At3g48780 serine palmitoyltransferase-like protein 28 0.75
At4g12750 putative protein 27 2.2
At1g60980 putative gibberellin 20-oxidase 26 2.8
At3g27910 hypothetical protein 25 4.9
At5g40830 unknown protein 25 8.3
At3g49460 putative protein 25 8.3
>At5g45041 unknown protein
Length = 175
Score = 195 bits (495), Expect = 4e-51
Identities = 85/108 (78%), Positives = 101/108 (92%)
Query: 1 SIAQTIDIQRGATLFSQTCIGCHDAGGNIIQPGSTLFTKDLQRNGVDTEEEIYRVTYYGK 60
S+ QT+DIQRGATLF++ CIGCHD GGNIIQPG+TLFTKDL+RNGVDTEEEIYRVTY+GK
Sbjct: 68 SLGQTLDIQRGATLFNRACIGCHDTGGNIIQPGATLFTKDLERNGVDTEEEIYRVTYFGK 127
Query: 61 GRMPGFGKECMPRGQCTFGARLEDEDIKILAEFVKLQADKGWPSIETE 108
GRMPGFG++C PRGQCTFG RL+DE+IK+LAEFVK QAD+GWP++ T+
Sbjct: 128 GRMPGFGEKCTPRGQCTFGPRLQDEEIKLLAEFVKFQADQGWPTVSTD 175
>At5g23670 serine palmitoyltransferase
Length = 489
Score = 28.1 bits (61), Expect = 0.75
Identities = 12/46 (26%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
Query: 30 IQPGSTLFTKDLQRNGV----DTEEEIYRVTYYGKGRMPGFGKECM 71
I+ S F +LQ+ G D + + + Y ++P F +EC+
Sbjct: 374 IRENSNFFRAELQKMGFEVLGDNDSPVMPIMLYNPAKIPAFSRECL 419
>At3g48780 serine palmitoyltransferase-like protein
Length = 489
Score = 28.1 bits (61), Expect = 0.75
Identities = 12/46 (26%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
Query: 30 IQPGSTLFTKDLQRNGV----DTEEEIYRVTYYGKGRMPGFGKECM 71
I+ S F +LQ+ G D + + + Y ++P F +EC+
Sbjct: 374 IRENSNFFRAELQKMGFEVLGDNDSPVMPIMLYNPAKIPAFSRECL 419
>At4g12750 putative protein
Length = 1108
Score = 26.6 bits (57), Expect = 2.2
Identities = 16/55 (29%), Positives = 23/55 (41%)
Query: 28 NIIQPGSTLFTKDLQRNGVDTEEEIYRVTYYGKGRMPGFGKECMPRGQCTFGARL 82
+++ GST+ +DL R D IY GK + + PRG G L
Sbjct: 652 DLLSSGSTIRMEDLPRAVADCAPSIYSHGSGGKIKRSSSNQYSYPRGSWVHGGEL 706
>At1g60980 putative gibberellin 20-oxidase
Length = 376
Score = 26.2 bits (56), Expect = 2.8
Identities = 21/67 (31%), Positives = 28/67 (41%), Gaps = 12/67 (17%)
Query: 43 RNGVDTEEEIYRVTYYGKGRMP----GFGKECMPRGQCTFGARLEDEDIKILAEFVKLQA 98
R + E I+R+ YY K + P G G C P T L+ + + L FV
Sbjct: 217 REFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDP----TSLTILQQDQVSGLQVFV---- 268
Query: 99 DKGWPSI 105
D W SI
Sbjct: 269 DNQWQSI 275
>At3g27910 hypothetical protein
Length = 294
Score = 25.4 bits (54), Expect = 4.9
Identities = 22/71 (30%), Positives = 34/71 (46%), Gaps = 6/71 (8%)
Query: 33 GSTLFTKDLQRNGVDTEEEIYRVTYYGKG-RMPGF--GKECMPRGQCTFGARLEDEDIKI 89
GS + L G D+ + IYRV Y R+ F G E +P+ T G+R+ + K+
Sbjct: 178 GSCVIDNMLYTFGFDSVKRIYRVRVYDPSVRVWSFVKGIEDIPKMDGTLGSRMANHGGKL 237
Query: 90 LAEFVKLQADK 100
+ + L DK
Sbjct: 238 V---ILLNLDK 245
>At5g40830 unknown protein
Length = 414
Score = 24.6 bits (52), Expect = 8.3
Identities = 24/86 (27%), Positives = 42/86 (47%), Gaps = 15/86 (17%)
Query: 16 SQTCIGCHDAGGNIIQPGSTLFTKD--LQRNGVDTEEEIYRVTYYGKGRMP-GFGKECMP 72
S+ C+GC D +T KD ++ NG T+ I V G G++ GF +
Sbjct: 223 SRDCVGCFDL--------ATSHEKDRFVKVNG-KTDFLIDDVLDLGDGKIRIGFD---IS 270
Query: 73 RGQCTFGARLEDEDIKILAEFVKLQA 98
G TF AR+ ++++ I++ + + A
Sbjct: 271 SGSGTFAARMAEKNVNIISNTLNIDA 296
>At3g49460 putative protein
Length = 46
Score = 24.6 bits (52), Expect = 8.3
Identities = 12/37 (32%), Positives = 19/37 (50%), Gaps = 2/37 (5%)
Query: 76 CTFGARLEDEDIKI--LAEFVKLQADKGWPSIETEQK 110
CT+ A L + + + L + L + WPS+ EQK
Sbjct: 8 CTYAALLLHDHVTMSTLVKTANLNIESYWPSLPPEQK 44
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,548,557
Number of Sequences: 26719
Number of extensions: 106886
Number of successful extensions: 148
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 146
Number of HSP's gapped (non-prelim): 8
length of query: 110
length of database: 11,318,596
effective HSP length: 86
effective length of query: 24
effective length of database: 9,020,762
effective search space: 216498288
effective search space used: 216498288
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC149576.1