BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149496.4 - phase: 0
(475 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g49055 putative protein 35 0.12
At3g52900 unknown protein 33 0.26
At5g66030 Golgi-localized protein GRIP 31 1.3
At2g35540 putative DnaJ protein 31 1.3
At5g36100 putative protein 31 1.7
At4g14760 centromere protein homolog 30 2.2
At3g50060 R2R3-MYB transcription factor 30 2.2
At1g17930 unknown protein 30 2.2
At5g24880 glutamic acid-rich protein 30 2.9
At3g12190 hypothetical protein 30 2.9
At2g27740 unknown protein 30 2.9
At2g26820 similar to avrRpt2-induced protein 1 30 2.9
At1g65040 30 2.9
At3g61570 unknown protein 30 3.8
At5g60930 microtubule-associated motor - like 29 5.0
At2g18540 putative vicilin storage protein (globulin-like) 29 5.0
At1g17140 unknown protein 29 5.0
At5g60030 KED - like protein 29 6.5
At5g43560 unknown protein 29 6.5
At4g11080 98b like protein 29 6.5
>At3g49055 putative protein
Length = 480
Score = 34.7 bits (78), Expect = 0.12
Identities = 24/81 (29%), Positives = 38/81 (46%), Gaps = 10/81 (12%)
Query: 385 LPQTKEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNER 444
L Q EE + + EAE E+ W+ C + E G E E +D +IA L
Sbjct: 323 LAQNVEELVKAIREAESEVSRWREAC-----ELEVEAGQREVEV-----RDQLIAVLKSE 372
Query: 445 IKEKDAALDRIPGRKKKRMDL 465
+++ +AL R G+ K + +L
Sbjct: 373 VEKLRSALARSEGKLKLKEEL 393
>At3g52900 unknown protein
Length = 164
Score = 33.5 bits (75), Expect = 0.26
Identities = 20/81 (24%), Positives = 44/81 (53%), Gaps = 5/81 (6%)
Query: 371 RFLSSTVPEIPLPLLPQTKEE--YQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEA 428
R L+ T+ E+ + P KE ++++ A R+I + CQKK+K+Y+ + ++
Sbjct: 69 RCLTQTLEELEIMGDPMRKEVGMVRKKIDMANRDIKSLAQSCQKKEKEYKDTLEAFNEK- 127
Query: 429 YDSRQKDVIIAKLNERIKEKD 449
+++K +++ L E + E +
Sbjct: 128 --NKEKAHLVSMLMELLAESE 146
>At5g66030 Golgi-localized protein GRIP
Length = 788
Score = 31.2 bits (69), Expect = 1.3
Identities = 17/68 (25%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Query: 393 QERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEKDAAL 452
+E L EAE+E+++ E + +D ++ + LE+E ++ + +E+IK + L
Sbjct: 462 EEALKEAEKEVYLVSAERDRAQQDLQSALASLEKEL---EERAGALKDASEQIKSLEVKL 518
Query: 453 DRIPGRKK 460
D R +
Sbjct: 519 DSTVARNQ 526
>At2g35540 putative DnaJ protein
Length = 575
Score = 31.2 bits (69), Expect = 1.3
Identities = 23/77 (29%), Positives = 40/77 (51%), Gaps = 8/77 (10%)
Query: 391 EYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEKDA 450
E E EA I+++ + D++ E +M L E +A R K +K+N +I EKD+
Sbjct: 215 ETGEMSQEAAEAINVF----EGSDEEDEGMMTLAEMQAVIKRNK----SKVNSKITEKDS 266
Query: 451 ALDRIPGRKKKRMDLFD 467
+ + GR+ ++ L D
Sbjct: 267 SGEENMGRETQKRSLAD 283
>At5g36100 putative protein
Length = 236
Score = 30.8 bits (68), Expect = 1.7
Identities = 19/92 (20%), Positives = 47/92 (50%), Gaps = 6/92 (6%)
Query: 383 PLLPQTKEEYQERLAEAE-----REIHMWKRECQKKDKDYETVM-GLLEQEAYDSRQKDV 436
P+L Q +EE +A E +E+ W + + K+ +++ G++ ++ ++ KD
Sbjct: 112 PVLVQIEEETTIAIAYREDEDMTKELKSWLESIRDEGKNNQSLYRGVILEKGFEEEDKDQ 171
Query: 437 IIAKLNERIKEKDAALDRIPGRKKKRMDLFDG 468
I + + + A L+ + G+K++ + + +G
Sbjct: 172 SIVPRDAKSENVRAKLEDLLGKKQESVTIHEG 203
>At4g14760 centromere protein homolog
Length = 1676
Score = 30.4 bits (67), Expect = 2.2
Identities = 16/65 (24%), Positives = 37/65 (56%), Gaps = 6/65 (9%)
Query: 389 KEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDV-IIAKLNERIKE 447
K + RLA+ ++ + + EC+ + ++YE E + ++Q ++ I+ KL E +++
Sbjct: 748 KRSTESRLADLQKNVSFLREECRSRKREYED-----ELDRVVNKQVEIFILQKLIEDLEQ 802
Query: 448 KDAAL 452
K+ +L
Sbjct: 803 KNFSL 807
>At3g50060 R2R3-MYB transcription factor
Length = 301
Score = 30.4 bits (67), Expect = 2.2
Identities = 22/80 (27%), Positives = 41/80 (50%), Gaps = 5/80 (6%)
Query: 104 KNATIAEALHLKPSDLVHPHF--TIKNNLQG---LTAKFLYQKASDFVKAKKTNAFESIF 158
K ATIA L+ + + V H+ T+K G +T ++ D K +++ +F+S F
Sbjct: 79 KWATIARLLNGRTDNAVKNHWNSTLKRKCSGGVAVTTVTETEEDQDRPKKRRSVSFDSAF 138
Query: 159 TLLIYGLFLFPNMDNFVDLN 178
+ GL++ P N +D++
Sbjct: 139 APVDTGLYMSPESPNGIDVS 158
>At1g17930 unknown protein
Length = 478
Score = 30.4 bits (67), Expect = 2.2
Identities = 43/194 (22%), Positives = 80/194 (41%), Gaps = 25/194 (12%)
Query: 47 VKVEDGILETLVQFYDPICHCFTFPDYQLVPTLEEYSFWVGLPVSEGEPFNGLEPSPKNA 106
+ + + ++ LV+ + + F FP ++ TL+E S +GL V +G+P G++ ++
Sbjct: 60 ISLNNSLISALVERWRRETNTFHFPCGEMTITLDEVSLILGLAV-DGKPVVGVKEKDEDP 118
Query: 107 T--IAEALHLKPSDLVHPHFTIKNNLQG--LTAKFLYQKASDFVKA---KKTNAFESIFT 159
+ L P K L G +TAK+L + ++ K K+ +
Sbjct: 119 SQVCLRLLGKLP----------KGELSGNRVTAKWLKESFAECPKGATMKEIEYHTRAYL 168
Query: 160 LLIYGLFLFPNMD-NFVDLNAIKIFLAKNPV------PTLLANTYHSIHHRNIREGGLIV 212
+ I G +F D + + ++ + +F LA Y I + + R +I
Sbjct: 169 IYIVGSTIFATTDPSKISVDYLILFEDFEKAGEYAWGAAALAFLYRQIGNASQRSQSIIG 228
Query: 213 CCAPLLYRWYASHL 226
C LL W HL
Sbjct: 229 GCLTLLQCWSYFHL 242
>At5g24880 glutamic acid-rich protein
Length = 443
Score = 30.0 bits (66), Expect = 2.9
Identities = 21/84 (25%), Positives = 41/84 (48%), Gaps = 9/84 (10%)
Query: 387 QTKEEYQERLAEAEREIHMWKRECQK---KDKDYETVMG------LLEQEAYDSRQKDVI 437
+ KEE +ER+ E E+E K + QK ++++ E V G + E+E+ + ++K+V+
Sbjct: 338 EVKEEGKERVEEEEKEKEKVKEDDQKEKVEEEEKEKVKGDEEKEKVKEEESAEGKKKEVV 397
Query: 438 IAKLNERIKEKDAALDRIPGRKKK 461
K D ++ +K
Sbjct: 398 KGKKESPSAYNDVIASKMQENPRK 421
>At3g12190 hypothetical protein
Length = 269
Score = 30.0 bits (66), Expect = 2.9
Identities = 27/104 (25%), Positives = 48/104 (45%), Gaps = 16/104 (15%)
Query: 388 TKEEYQERLAEAER--EIHMWKRECQKKDKDYETVMGLLE--------------QEAYDS 431
+KE +E L + E+ EI K+ +KK KD + L E +E
Sbjct: 153 SKEMSEELLVQQEKYEEILKKKKLEEKKLKDCTRDLALREGDLRWVSMRMTKRCEELRWE 212
Query: 432 RQKDVIIAKLNERIKEKDAALDRIPGRKKKRMDLFDGPHSDFED 475
++K++++ K NE + K L+R K+K +DL + ++ D
Sbjct: 213 KKKNLVLCKRNEEAERKLKHLNRALEEKQKEVDLIEKRLGEWRD 256
>At2g27740 unknown protein
Length = 174
Score = 30.0 bits (66), Expect = 2.9
Identities = 21/90 (23%), Positives = 44/90 (48%), Gaps = 9/90 (10%)
Query: 364 GMPYTLPRFLSSTVPEIPLPLLPQTKE--EYQERLAEAEREIHMWKRECQKKDKDYETVM 421
G+ R L+ E+ P KE ++R+ RE+ + CQ+K+++++ +
Sbjct: 71 GLAEEATRRLAEIREELEALTDPMRKEISAIRKRVDAINRELKPLGQSCQRKEREFKEAL 130
Query: 422 GLLEQEAYDSRQKD--VIIAKLNERIKEKD 449
EAY+ + K+ + ++KL E + E +
Sbjct: 131 -----EAYNEKNKEKAIFVSKLVELVTESE 155
>At2g26820 similar to avrRpt2-induced protein 1
Length = 463
Score = 30.0 bits (66), Expect = 2.9
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Query: 389 KEEYQERLAEAEREIHMWKR-ECQKKDKDYETVMGLLEQEAYDSRQKDVII-AKLNERIK 446
KEE ERL E ER I R E + + +M + + +++ K +I A+ NE++
Sbjct: 211 KEENDERLREEERVIESKNRAEAELAEMQQNLLMEKEKLQMEEAKNKQLIAQAEANEKLM 270
Query: 447 EKDAALDR 454
E++ A +R
Sbjct: 271 EQERAKNR 278
>At1g65040
Length = 537
Score = 30.0 bits (66), Expect = 2.9
Identities = 24/86 (27%), Positives = 40/86 (45%), Gaps = 2/86 (2%)
Query: 349 HESYTQWVIDRATAFGMPYTLPRFLSSTVPEIPLPLLPQTKEEYQERLAEAEREIHMWKR 408
HE+ Q A+ +G P ++ V LLPQT+ E Q R E++ E +
Sbjct: 381 HEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQTEVEAQRRFLESQIEDILVSN 440
Query: 409 ECQKKDKDYETVMGLLEQEAYDSRQK 434
KD+ ++++ L +EA +R K
Sbjct: 441 FV--KDEAMKSLIWLTAEEAAKNRGK 464
>At3g61570 unknown protein
Length = 712
Score = 29.6 bits (65), Expect = 3.8
Identities = 19/66 (28%), Positives = 28/66 (41%), Gaps = 10/66 (15%)
Query: 389 KEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEK 448
KE+ +L AE+ WK K ++D V +LEQ + +LN E
Sbjct: 513 KEDVTSKLLHAEKVAAEWKNRVTKVEEDNAKVRRVLEQS----------MTRLNRMSMES 562
Query: 449 DAALDR 454
D +DR
Sbjct: 563 DYLVDR 568
>At5g60930 microtubule-associated motor - like
Length = 647
Score = 29.3 bits (64), Expect = 5.0
Identities = 17/85 (20%), Positives = 43/85 (50%), Gaps = 3/85 (3%)
Query: 385 LPQTKEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNER 444
+ Q E+++ A E+E+ K+E ++ + + +M L +++ ++K +++ +R
Sbjct: 32 IKQESEQFRAWKASREKEVMQLKKEGRRNEYEMHKLMALNQKQKLVLQRKTEEASQVTKR 91
Query: 445 IKEKDAALDRIPGRKKKRMDLFDGP 469
+KE LD ++ + +GP
Sbjct: 92 LKE---LLDNRKASSRETLSGANGP 113
>At2g18540 putative vicilin storage protein (globulin-like)
Length = 699
Score = 29.3 bits (64), Expect = 5.0
Identities = 19/67 (28%), Positives = 38/67 (56%), Gaps = 1/67 (1%)
Query: 389 KEEYQERLAEAEREIHMWK-RECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKE 447
+EE ++R E +RE M K RE +++ K+ E V + +E R++++ + ER K+
Sbjct: 551 EEEARKREEERKREEEMAKRREQERQRKEREEVERKIREEQERKREEEMAKRREQERQKK 610
Query: 448 KDAALDR 454
+ ++R
Sbjct: 611 EREEMER 617
>At1g17140 unknown protein
Length = 344
Score = 29.3 bits (64), Expect = 5.0
Identities = 19/80 (23%), Positives = 37/80 (45%), Gaps = 5/80 (6%)
Query: 391 EYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEKDA 450
+ + +L +A+ E+ + K + E V + E + +K +A++ E E +
Sbjct: 69 DLESQLGQAQEELRLLKEQLANA----EAVKKQAQDELHKKSKKPNPLARVEESATEAER 124
Query: 451 A-LDRIPGRKKKRMDLFDGP 469
D IPG +K D+F+ P
Sbjct: 125 IDRDEIPGDVQKETDVFEVP 144
>At5g60030 KED - like protein
Length = 292
Score = 28.9 bits (63), Expect = 6.5
Identities = 19/84 (22%), Positives = 40/84 (47%), Gaps = 8/84 (9%)
Query: 387 QTKEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDS--------RQKDVII 438
+ KE+ ++ A+R+ K+ + D+D LE E + + +DV+
Sbjct: 168 KVKEKLEDEQKSADRKERKKKKSKKNNDEDVVDEKEKLEDEQKSAEIKEKKKNKDEDVVD 227
Query: 439 AKLNERIKEKDAALDRIPGRKKKR 462
K E+++++ + +R +KKKR
Sbjct: 228 EKEKEKLEDEQRSGERKKEKKKKR 251
Score = 28.5 bits (62), Expect = 8.4
Identities = 17/72 (23%), Positives = 36/72 (49%), Gaps = 13/72 (18%)
Query: 390 EEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEKD 449
E+ E+L +R +R+ +KK K +++ +DV+ K+ E+++++
Sbjct: 131 EKVNEKLEAEQRSEERRERKKEKKKKK-------------NNKDEDVVDEKVKEKLEDEQ 177
Query: 450 AALDRIPGRKKK 461
+ DR +KKK
Sbjct: 178 KSADRKERKKKK 189
>At5g43560 unknown protein
Length = 1055
Score = 28.9 bits (63), Expect = 6.5
Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Query: 389 KEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEK 448
+EE + LAE+E++ E +KK K + + + + R++D + + ER EK
Sbjct: 423 REEEEAWLAESEQKGKRGASEKEKKSKKKQAKQKKNKNKGKEMRKEDKVRTQTEEREIEK 482
Query: 449 DAALDRIPGRKKKRMDLFDGPHSDFED 475
+ + I ++ D G SD D
Sbjct: 483 EECVRAIAESSAEKPDTL-GDVSDVSD 508
>At4g11080 98b like protein
Length = 446
Score = 28.9 bits (63), Expect = 6.5
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 13/90 (14%)
Query: 385 LPQTKEEYQERLAEAEREIHMWKRECQKKDKDYETV---------MGLLEQEAYDSRQK- 434
L + K+ +++A+ +EI++ + E K+ K+ E + M L +QEA +K
Sbjct: 285 LSEEKKAPYDQMAKKNKEIYLQEMEGYKRTKEEEAMSQKKEEEEFMKLHKQEALQLLKKK 344
Query: 435 ---DVIIAKLNERIKEKDAALDRIPGRKKK 461
D II K E K K + P + KK
Sbjct: 345 EKTDNIIKKTKETAKNKKKNENVDPNKPKK 374
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.138 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,314,474
Number of Sequences: 26719
Number of extensions: 515714
Number of successful extensions: 1396
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 1377
Number of HSP's gapped (non-prelim): 43
length of query: 475
length of database: 11,318,596
effective HSP length: 103
effective length of query: 372
effective length of database: 8,566,539
effective search space: 3186752508
effective search space used: 3186752508
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149496.4