BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149471.20 + phase: 0
(227 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g43110 unknown protein 217 5e-57
At1g31970 unknown protein 39 0.002
At3g06980 putative DEAD/DEAH box helicase 38 0.005
At3g22310 putative RNA helicase 37 0.009
At3g22330 DEAD-Box RNA helicase like protein 36 0.015
At5g62190 putative RNA helicase (MMI9.2) 35 0.042
At5g26742 unknown protein 33 0.16
At5g52580 unknown protein 32 0.36
At3g09720 RNA helicase like protein 32 0.36
At5g01370 unknown protein 31 0.61
At3g19760 RNA helicase, putative 31 0.61
At4g35940 putative protein 30 0.80
At4g33370 putative protein 30 0.80
At5g51280 DEAD-box protein abstrakt 30 1.0
At5g14610 DRH1 DEAD box protein - like 30 1.4
At5g08700 putative protein 30 1.4
At3g53110 RNA helicase -like protein 29 1.8
At2g47330 putative ATP-dependent RNA helicase 29 1.8
At4g28620 ABC transporter - like protein 29 2.3
At1g51380 putative RNA helicase 29 2.3
>At2g43110 unknown protein
Length = 288
Score = 217 bits (552), Expect = 5e-57
Identities = 111/201 (55%), Positives = 147/201 (72%), Gaps = 6/201 (2%)
Query: 4 RKGRENNDDRRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILELSN 63
R + D+ ++ + SEQL +F+N SA +++SS+ELE +K D+ I+ELS
Sbjct: 55 RSNDQKTDNDEDEQLYSEPVSASEQLNYFLNHLDSAIGIKVSSLELEPIK-DTCIVELSQ 113
Query: 64 SQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRSIHL 123
D DV LG IK + G+ WR+ LCE E +E K+ PG+PSVL++S SALRS+ L
Sbjct: 114 G-----LDQDVSNLGEHIKLSCGSSWRETLCEGESLERKVEPGNPSVLVISSSALRSLEL 168
Query: 124 LKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQV 183
L+G +TKQC AVKLFSKH+K++EQ+SLLK RVNI SGTP+RIKKL+D+EALGLSRL +
Sbjct: 169 LRGLHSLTKQCPAVKLFSKHLKVEEQVSLLKKRVNIGSGTPNRIKKLVDIEALGLSRLDM 228
Query: 184 LVLDMHPDVKGYSLFTLPQVR 204
+V+DMHPDVKG+SLFTLPQVR
Sbjct: 229 IVIDMHPDVKGFSLFTLPQVR 249
>At1g31970 unknown protein
Length = 537
Score = 39.3 bits (90), Expect = 0.002
Identities = 27/98 (27%), Positives = 53/98 (53%), Gaps = 3/98 (3%)
Query: 92 VLCESEVVEGKIPPGSPSVLIVSPSALRSIHLLKGFRFMTKQCS--AVKLFSKHIKLQEQ 149
VL +++ + G +P+ L++SP+ ++ + R + C ++ ++ K Q
Sbjct: 176 VLKKNKKIGGGSKKVNPTCLVLSPTRELAVQISDVLREAGEPCGLKSICVYGGSSK-GPQ 234
Query: 150 ISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
IS +++ V+I GTP R++ LI+ L LS + +VLD
Sbjct: 235 ISAIRSGVDIVIGTPGRLRDLIESNVLRLSDVSFVVLD 272
>At3g06980 putative DEAD/DEAH box helicase
Length = 781
Score = 37.7 bits (86), Expect = 0.005
Identities = 24/88 (27%), Positives = 45/88 (50%), Gaps = 2/88 (2%)
Query: 102 KIPPGSPSVLIVSPSALRSIHLLKGFRFMTKQCSAVK--LFSKHIKLQEQISLLKNRVNI 159
K PG P V+++ P+A + +L R ++K + + + + + Q+ L+ V++
Sbjct: 446 KSSPGCPRVIVLVPTAELASQVLANCRSISKSGVPFRSMVVTGGFRQRTQLENLEQGVDV 505
Query: 160 ASGTPSRIKKLIDVEALGLSRLQVLVLD 187
TP R L++ LGLS L+ +LD
Sbjct: 506 LIATPGRFTYLMNEGILGLSNLRCAILD 533
>At3g22310 putative RNA helicase
Length = 610
Score = 37.0 bits (84), Expect = 0.009
Identities = 24/81 (29%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
Query: 107 SPSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSR 166
+P L+++P+ + + K FR + L+ + +Q+ L +++A GTP R
Sbjct: 190 NPQCLVLAPTRELARQVEKEFRESAPSLDTICLYGG-TPIGQQMRELNYGIDVAVGTPGR 248
Query: 167 IKKLIDVEALGLSRLQVLVLD 187
I L+ AL LS +Q +VLD
Sbjct: 249 IIDLMKRGALNLSEVQFVVLD 269
>At3g22330 DEAD-Box RNA helicase like protein
Length = 616
Score = 36.2 bits (82), Expect = 0.015
Identities = 24/81 (29%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
Query: 107 SPSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSR 166
+P L+++P+ + + K FR + L+ + +Q+ L V++A GTP R
Sbjct: 178 NPLCLVLAPTRELARQVEKEFRESAPSLDTICLYGG-TPIGQQMRQLDYGVDVAVGTPGR 236
Query: 167 IKKLIDVEALGLSRLQVLVLD 187
+ L+ AL LS +Q +VLD
Sbjct: 237 VIDLMKRGALNLSEVQFVVLD 257
>At5g62190 putative RNA helicase (MMI9.2)
Length = 671
Score = 34.7 bits (78), Expect = 0.042
Identities = 58/201 (28%), Positives = 84/201 (40%), Gaps = 19/201 (9%)
Query: 3 KRKGRENNDDRR------KKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADS 56
KRK E D+ + KKK K G E + V+ + + ++S+ E LKA+
Sbjct: 58 KRKASEEEDEVKSDSSSEKKKSSKKVKLGVEDVE--VDNPNAVSKFRISAPLREKLKANG 115
Query: 57 SILELSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESE-VVEG------KIPPG-SP 108
++ D LD L G + G VL E +V G K+ G SP
Sbjct: 116 IEALFPIQASTFDMVLDGADLVGRARTGQGKTLAFVLPILESLVNGPAKSKRKMGYGRSP 175
Query: 109 SVLIVSPSALRSIHLLKGFRFM--TKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSR 166
SVL++ P+ + + F + S+ L+ Q LK V+I GTP R
Sbjct: 176 SVLVLLPTRELAKQVAADFDAYGGSLGLSSCCLYGGD-SYPVQEGKLKRGVDIVVGTPGR 234
Query: 167 IKKLIDVEALGLSRLQVLVLD 187
IK I+ + L S LQ VLD
Sbjct: 235 IKDHIERQNLDFSYLQFRVLD 255
>At5g26742 unknown protein
Length = 475
Score = 32.7 bits (73), Expect = 0.16
Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 1/80 (1%)
Query: 108 PSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRI 167
P L+++P+ + + K + S V ++ + Q S L V++ GTP RI
Sbjct: 181 PKFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGG-VSYTIQQSALTRGVDVVVGTPGRI 239
Query: 168 KKLIDVEALGLSRLQVLVLD 187
LI+ +L L ++ LVLD
Sbjct: 240 IDLIEGRSLKLGEVEYLVLD 259
>At5g52580 unknown protein
Length = 327
Score = 31.6 bits (70), Expect = 0.36
Identities = 36/128 (28%), Positives = 57/128 (44%), Gaps = 29/128 (22%)
Query: 24 TGSEQLRFFVNEFQSANDVQLSSIELES---LKADSSILEL---SNSQNSRDTDLDVKLL 77
+ +Q F VN+FQS LSS+EL S + + S+ L S+S+N R T DV
Sbjct: 133 SSEDQNVFIVNDFQSPLQRTLSSLELPSSLPVASGQSVYPLDGGSSSENQRRTSSDV--- 189
Query: 78 GGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRSIHLLKGFRFMTK--QCS 135
G + + +R+ P+ SIHLL+ F +TK + +
Sbjct: 190 GNRVSSVSQSGFRKQKSH------------------DPTRDLSIHLLEKFSLVTKFARDT 231
Query: 136 AVKLFSKH 143
+LFS++
Sbjct: 232 TTQLFSEN 239
>At3g09720 RNA helicase like protein
Length = 541
Score = 31.6 bits (70), Expect = 0.36
Identities = 29/99 (29%), Positives = 48/99 (48%), Gaps = 10/99 (10%)
Query: 107 SPSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSR 166
SP+ + + +A L+KG F ++L +K + S L V I+ TP R
Sbjct: 217 SPARELAAQTAREGKKLIKGSNFH------IRLMTKPLVKTADFSKLWCDVLIS--TPMR 268
Query: 167 IKKLIDVEALGLSRLQVLVLDMHPDVKGYSLFTLPQVRC 205
+K+ I + + LS+++ LVLD + SL L Q+ C
Sbjct: 269 LKRAIKAKKIDLSKVEYLVLDESDKLFEQSL--LKQIDC 305
>At5g01370 unknown protein
Length = 427
Score = 30.8 bits (68), Expect = 0.61
Identities = 15/71 (21%), Positives = 42/71 (59%), Gaps = 2/71 (2%)
Query: 13 RRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSI--ELESLKADSSILELSNSQNSRDT 70
++KK + + + G++++R+F++ +S + + +++ E ++++AD I + S S ++
Sbjct: 233 KKKKSENRNDVNGAKKVRWFLSPSKSKSSSEKTALLGESKNIRADEFIGKESESPENKSN 292
Query: 71 DLDVKLLGGDI 81
V +L D+
Sbjct: 293 ASPVSVLDRDL 303
>At3g19760 RNA helicase, putative
Length = 408
Score = 30.8 bits (68), Expect = 0.61
Identities = 27/112 (24%), Positives = 51/112 (45%), Gaps = 14/112 (12%)
Query: 90 RQVLCESEVVEGKIPPGSPSVLIVSPSALRSIH--LLKGFRFMTKQCS-AVKLFSKHIKL 146
R V+ +++ GK + SV V ++ R + +L R + Q ++ H +
Sbjct: 73 RDVIAQAQSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHANI 132
Query: 147 Q-----------EQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
Q E I L++ V++ SGTP R+ +I +L +++L+LD
Sbjct: 133 QAHACIGGNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILD 184
>At4g35940 putative protein
Length = 451
Score = 30.4 bits (67), Expect = 0.80
Identities = 22/84 (26%), Positives = 40/84 (47%), Gaps = 14/84 (16%)
Query: 3 KRKGRENNDDRRKKKKKKTETTGSE-------------QLRFFVNEFQSANDVQLSSIEL 49
K+ +E + + KK+KK+ E G E + V+ F + +++ +E
Sbjct: 50 KKDKKERKEKKEKKEKKRKEREGKEVGSEKRSHKRRRKEDGAKVDLFHKLKESEVNCLEK 109
Query: 50 ESLKADSSILELSNSQNSRDTDLD 73
SL + +L+ S SQNS D+ L+
Sbjct: 110 SSLTVERELLQ-STSQNSCDSTLN 132
>At4g33370 putative protein
Length = 542
Score = 30.4 bits (67), Expect = 0.80
Identities = 12/44 (27%), Positives = 26/44 (58%)
Query: 144 IKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
+ ++ Q+ ++K V+I TP R+K ++ + + L ++L LD
Sbjct: 217 VDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMSLDACRLLTLD 260
>At5g51280 DEAD-box protein abstrakt
Length = 591
Score = 30.0 bits (66), Expect = 1.0
Identities = 13/44 (29%), Positives = 25/44 (56%)
Query: 144 IKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
I ++ Q+ ++K V+I TP R+K ++ + + L + L LD
Sbjct: 266 IDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMSLDACRYLTLD 309
>At5g14610 DRH1 DEAD box protein - like
Length = 713
Score = 29.6 bits (65), Expect = 1.4
Identities = 19/82 (23%), Positives = 45/82 (54%), Gaps = 3/82 (3%)
Query: 108 PSVLIVSPSALRSIHL-LKGFRF-MTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPS 165
P++L++SP+ + + ++ +F + + S L+ K Q+ ++ V+I TP
Sbjct: 302 PTILVLSPTRELATQIQVEALKFGKSSKISCACLYGGAPK-GPQLKEIERGVDIVVATPG 360
Query: 166 RIKKLIDVEALGLSRLQVLVLD 187
R+ +++++ + L ++ LVLD
Sbjct: 361 RLNDILEMKRISLHQVSYLVLD 382
>At5g08700 putative protein
Length = 768
Score = 29.6 bits (65), Expect = 1.4
Identities = 19/57 (33%), Positives = 28/57 (48%), Gaps = 5/57 (8%)
Query: 126 GFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQ 182
GFR K A+ F+K+ K QIS+L +A P R + +D A G ++Q
Sbjct: 78 GFRSEQKAQDAITYFNKNFKHSHQISVL-----VADPPPRRTQGKVDAYAKGDKQIQ 129
>At3g53110 RNA helicase -like protein
Length = 496
Score = 29.3 bits (64), Expect = 1.8
Identities = 12/30 (40%), Positives = 20/30 (66%)
Query: 158 NIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
++ GTP +KK + + LGL+ L++LV D
Sbjct: 216 HVVIGTPGTLKKWMAFKRLGLNHLKILVFD 245
>At2g47330 putative ATP-dependent RNA helicase
Length = 760
Score = 29.3 bits (64), Expect = 1.8
Identities = 23/64 (35%), Positives = 31/64 (47%), Gaps = 6/64 (9%)
Query: 141 SKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLDMHPDVKGYSLFTL 200
SKH EQ LK I TP R+ ++ ++AL + R LVLD + + L
Sbjct: 340 SKH----EQFKELKAGCEIVVATPGRLIDMLKMKALTMMRASYLVLDEAD--RMFDLGFE 393
Query: 201 PQVR 204
PQVR
Sbjct: 394 PQVR 397
>At4g28620 ABC transporter - like protein
Length = 680
Score = 28.9 bits (63), Expect = 2.3
Identities = 25/97 (25%), Positives = 47/97 (47%), Gaps = 7/97 (7%)
Query: 69 DTDLDVKLLGGDIKG---AFGNCWRQVLCESEVVEG---KIPPGSPSVLIVSPSALRSIH 122
D D+D KL +KG +F N L E ++++G ++P G ++ S + +S
Sbjct: 422 DKDIDRKLPPLVLKGGSISFENVHFSYLPERKILDGISFEVPAGKSVAIVGSSGSGKSTI 481
Query: 123 LLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNI 159
L FRF VK+ + IK + ++ L++ + +
Sbjct: 482 LRMIFRFFDVDSGNVKIDGQDIK-EVRLESLRSSIGV 517
>At1g51380 putative RNA helicase
Length = 392
Score = 28.9 bits (63), Expect = 2.3
Identities = 15/40 (37%), Positives = 23/40 (57%)
Query: 148 EQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
E I L+ V+ SGTP R+ +I +L +++LVLD
Sbjct: 132 EDIKKLERGVHAVSGTPGRVYDMIKRGSLQTKAVKLLVLD 171
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.134 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,924,561
Number of Sequences: 26719
Number of extensions: 200231
Number of successful extensions: 1017
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 986
Number of HSP's gapped (non-prelim): 46
length of query: 227
length of database: 11,318,596
effective HSP length: 96
effective length of query: 131
effective length of database: 8,753,572
effective search space: 1146717932
effective search space used: 1146717932
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC149471.20