BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149307.10 + phase: 0 /pseudo
(114 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g32110 non-LTR reverse transcriptase, putative 50 1e-07
At1g43760 reverse transcriptase, putative 45 4e-06
At2g07670 putative non-LTR retrolelement reverse transcriptase 42 4e-05
At1g10160 putative reverse transcriptase 40 2e-04
At2g14430 putative non-LTR retroelement reverse transcriptase 37 0.002
At2g18820 putative non-LTR retroelement reverse transcriptase 36 0.004
At2g05980 putative non-LTR retroelement reverse transcriptase 35 0.005
At1g24640 hypothetical protein 35 0.005
At2g15540 putative non-LTR retroelement reverse transcriptase 35 0.006
At2g31080 putative non-LTR retroelement reverse transcriptase 35 0.008
At2g05200 putative non-LTR retroelement reverse transcriptase 34 0.010
At3g31420 hypothetical protein 34 0.014
At3g25485 unknown protein 33 0.030
At3g30570 putative reverse transcriptase 32 0.039
At2g17910 putative non-LTR retroelement reverse transcriptase 31 0.088
At2g05550 putative non-LTR retroelement reverse transcriptase 31 0.088
At1g31100 hypothetical protein 31 0.088
At2g45230 putative non-LTR retroelement reverse transcriptase 31 0.11
At1g78500 Putative Oxidosqualene Cyclase 30 0.15
At1g25430 hypothetical protein 30 0.25
>At3g32110 non-LTR reverse transcriptase, putative
Length = 1911
Score = 50.4 bits (119), Expect = 1e-07
Identities = 32/112 (28%), Positives = 50/112 (44%), Gaps = 6/112 (5%)
Query: 3 ERKSRGSSFRQHDADIFNKFIDDSFLVDFPICGRLFTWYRGDG---YSMSKLDRFLLSDN 59
+ +S G+ D+ F ++I+D L+D G FTW RG + +LDR L +
Sbjct: 710 DERSGGNGRLSSDSLAFGEWINDHSLIDLGFKGNKFTWKRGREERFFVAKRLDRVLCCAH 769
Query: 60 CYSTWPNCIQIALQSGLSDHVHMVLRVDEE---NWGPRPLRMLKCWANFPGY 108
W + L SDH + +++ E N G RP R W + PG+
Sbjct: 770 ARLKWQEASVLHLPFLASDHAPLYVQLTPEVSGNRGRRPFRFEAAWLSHPGF 821
>At1g43760 reverse transcriptase, putative
Length = 622
Score = 45.4 bits (106), Expect = 4e-06
Identities = 28/89 (31%), Positives = 47/89 (52%), Gaps = 7/89 (7%)
Query: 19 FNKFIDDSFLVDFPICGRLFTW--YRGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGL 76
F + DS LVD P G +TW ++ D + KLDR + + + +S++P+ I + SG+
Sbjct: 248 FQNCLRDSDLVDIPSRGVHYTWSNHQDDNPIIRKLDRAIANGDWFSSFPSAIAVFELSGV 307
Query: 77 SDHVHMVLRVDEENWGPRPLRMLKCWANF 105
SDH ++ ++ P R KC+ F
Sbjct: 308 SDHSPCIIILEN-----LPKRSKKCFRYF 331
>At2g07670 putative non-LTR retrolelement reverse transcriptase
Length = 913
Score = 42.4 bits (98), Expect = 4e-05
Identities = 30/111 (27%), Positives = 46/111 (41%), Gaps = 6/111 (5%)
Query: 4 RKSRGSSFRQHDADIFNKFIDDSFLVDFPICGRLFTWYRG---DGYSMSKLDRFLLSDNC 60
RK+ G+ D+ F ++I++ L+D G FTW RG + +LDR L
Sbjct: 617 RKNGGNGGLSPDSLAFGEWINELSLIDMGFKGNKFTWKRGRVESTFVAKRLDRVLCRPQT 676
Query: 61 YSTWPNCIQIALQSGLSDHVHMVLRVDEE---NWGPRPLRMLKCWANFPGY 108
W L SDH + ++++ E N RP R W G+
Sbjct: 677 RLKWQEASVTHLPFFASDHAPIYIQLEPEVRSNPLRRPFRFEAAWLTHSGF 727
>At1g10160 putative reverse transcriptase
Length = 1118
Score = 39.7 bits (91), Expect = 2e-04
Identities = 21/67 (31%), Positives = 35/67 (51%), Gaps = 2/67 (2%)
Query: 25 DSFLVDFPICGRLFTW--YRGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGLSDHVHM 82
DS L D P G FTW ++ D + KLDR L + ++ +P+ + + G SDH
Sbjct: 174 DSQLSDLPSRGVFFTWSNHQQDNPILRKLDRALANGEWFAVFPSALAVFDPPGDSDHAPC 233
Query: 83 VLRVDEE 89
++ +D +
Sbjct: 234 IILIDNQ 240
>At2g14430 putative non-LTR retroelement reverse transcriptase
Length = 1277
Score = 37.0 bits (84), Expect = 0.002
Identities = 25/91 (27%), Positives = 41/91 (44%), Gaps = 3/91 (3%)
Query: 19 FNKFIDDSFLVDFPICGRLFTWY--RGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGL 76
F + + S L D G FTW R +G KLD+ L++D+ T+P+ + G
Sbjct: 414 FQEVVRYSSLTDLGYHGPRFTWCNKRENGLICKKLDKVLVNDSWCITFPHSYSVFEAGGC 473
Query: 77 SDHVHMVLRVDEEN-WGPRPLRMLKCWANFP 106
SDH + ++ G RP + + + P
Sbjct: 474 SDHTRGRIMLEAAAIGGRRPFKFVNVLTSIP 504
>At2g18820 putative non-LTR retroelement reverse transcriptase
Length = 1412
Score = 35.8 bits (81), Expect = 0.004
Identities = 22/55 (40%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Query: 28 LVDFPICGRLFTW--YRGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGLSDHV 80
L D G LFTW R +G KLDR LL+ S +P+ I G SDH+
Sbjct: 479 LEDMRTHGPLFTWGNKRNEGLICKKLDRVLLNPEYNSAYPHSYCIMDSGGCSDHL 533
>At2g05980 putative non-LTR retroelement reverse transcriptase
Length = 1352
Score = 35.4 bits (80), Expect = 0.005
Identities = 24/90 (26%), Positives = 39/90 (42%), Gaps = 3/90 (3%)
Query: 28 LVDFPICGRLFTWY--RGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGLSDHVHMVLR 85
L D G +FTW R +G KLDR L+++ +P I G+SDH+ ++
Sbjct: 414 LADMSYHGPVFTWCNKREEGLVNKKLDRVLVNEVWTRKFPAVYSIFEAGGISDHLRCRIK 473
Query: 86 VDEENWGPR-PLRMLKCWANFPGYALFVRE 114
N P+ P + P + V++
Sbjct: 474 FASGNTQPKGPFKFNNVLTTLPEFLQIVKD 503
>At1g24640 hypothetical protein
Length = 1270
Score = 35.4 bits (80), Expect = 0.005
Identities = 25/84 (29%), Positives = 38/84 (44%), Gaps = 2/84 (2%)
Query: 5 KSRGSSFRQHDADIFNKFIDDSFLVDFPICGRLFTW--YRGDGYSMSKLDRFLLSDNCYS 62
K+ G D FN+ I LV+ P G FTW RGD + +LDR + +
Sbjct: 109 KNGGPRRSDLDCKAFNEMIKGCDLVEMPAHGNGFTWAGRRGDHWIQCRLDRAFGNKEWFC 168
Query: 63 TWPNCIQIALQSGLSDHVHMVLRV 86
+P Q L SDH +++++
Sbjct: 169 FFPVSNQTFLDFRGSDHRPVLIKL 192
>At2g15540 putative non-LTR retroelement reverse transcriptase
Length = 1225
Score = 35.0 bits (79), Expect = 0.006
Identities = 32/122 (26%), Positives = 55/122 (44%), Gaps = 12/122 (9%)
Query: 5 KSRGSSFRQHDADI-FNKFIDDSFLVDFPICGRLFTWY--RGDGYSMSK--------LDR 53
+ RG R + + F I++ ++DFP G LF+W R G + K LDR
Sbjct: 145 EKRGGKKRSESSFLPFCCMIENCGMIDFPSTGSLFSWVGKRSCGVAGRKRRDLIKCRLDR 204
Query: 54 FLLSDNCYSTWPNCIQIALQSGLSDHVHMVLRVDEENWGP-RPLRMLKCWANFPGYALFV 112
+ ++ +S + + LQ SDH ++ + + + P + K W N PG+ V
Sbjct: 205 AMGNEEWHSIYSHTNVEYLQHRGSDHKPLLASIQNKPYRPYKHFIFDKRWINKPGFKESV 264
Query: 113 RE 114
+E
Sbjct: 265 QE 266
>At2g31080 putative non-LTR retroelement reverse transcriptase
Length = 1231
Score = 34.7 bits (78), Expect = 0.008
Identities = 25/103 (24%), Positives = 43/103 (41%), Gaps = 8/103 (7%)
Query: 3 ERKSRGSSFRQHDADIFNKFIDDSFLVDFPICGRLFTWYRGDGYS---MSKLDRFLLSDN 59
+ + G+ D+ F +I++ L+D G FTW RG S +LDR + +
Sbjct: 46 DERMGGNGRLSPDSLAFGDWINELSLIDLGFKGNKFTWRRGRQESTVVAKRLDRVFVCAH 105
Query: 60 CYSTWPNCIQIALQSGLSDHVHMVLRVDEENWGPRPLRMLKCW 102
W + L SDH + ++++ P R L+ W
Sbjct: 106 ARLKWQEAVVSHLPFMASDHAPLYVQLE-----PLQQRKLRKW 143
>At2g05200 putative non-LTR retroelement reverse transcriptase
Length = 1229
Score = 34.3 bits (77), Expect = 0.010
Identities = 26/91 (28%), Positives = 44/91 (47%), Gaps = 3/91 (3%)
Query: 1 MEERKSRGSSFRQHDADI-FNKFIDDSFLVDFPICGRLFTW--YRGDGYSMSKLDRFLLS 57
++ + RG R + I F FI + L D G F+W R D + +LDR + +
Sbjct: 39 LDNSEKRGGPPRDQGSFIDFRSFISKNGLWDLKYSGNPFSWRGMRYDWFVRQRLDRAMSN 98
Query: 58 DNCYSTWPNCIQIALQSGLSDHVHMVLRVDE 88
++ ++P+ L+ SDH +V+ VDE
Sbjct: 99 NSWLESFPSGRSEYLRFEGSDHRPLVVFVDE 129
>At3g31420 hypothetical protein
Length = 1491
Score = 33.9 bits (76), Expect = 0.014
Identities = 21/92 (22%), Positives = 39/92 (41%), Gaps = 3/92 (3%)
Query: 1 MEERKSRGSSFR-QHDADIFNKFIDDSFLVDFPICGRLFTWY--RGDGYSMSKLDRFLLS 57
+ R+ RG R + F + D G F+W RGD + LDR + +
Sbjct: 322 LSNREKRGGRLRPERTFQDFRNMVRGCNFTDLKSVGDRFSWAGKRGDHHVTCSLDRTMAN 381
Query: 58 DNCYSTWPNCIQIALQSGLSDHVHMVLRVDEE 89
+ ++ +P + ++ G SDH +V + +
Sbjct: 382 NEWHTLFPESETVFMEYGESDHRPLVTNISAQ 413
>At3g25485 unknown protein
Length = 979
Score = 32.7 bits (73), Expect = 0.030
Identities = 21/91 (23%), Positives = 41/91 (44%), Gaps = 6/91 (6%)
Query: 19 FNKFIDDSFLVDFPICGRLFTWY--RGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGL 76
F + ++ D G L+TW + + + KLDR L+++ ++P + G
Sbjct: 124 FQEMVNYCAFTDLSSHGSLYTWSNRQENDLILKKLDRVLVNNKWKMSFPQSYNVFEAGGC 183
Query: 77 SDH----VHMVLRVDEENWGPRPLRMLKCWA 103
SDH +++ + V E G +P + + A
Sbjct: 184 SDHLRCRINLNIDVGERVKGKKPFKFINAIA 214
>At3g30570 putative reverse transcriptase
Length = 1099
Score = 32.3 bits (72), Expect = 0.039
Identities = 30/121 (24%), Positives = 46/121 (37%), Gaps = 24/121 (19%)
Query: 3 ERKSRGSSFRQHDADIFNKFIDDSFLVDFPICGRLFTWY------------RGDGYSMSK 50
+ +S GS D+ F ++I+DS L+ TW R D + +
Sbjct: 194 DERSGGSGPLSPDSIAFGEWINDSSLL---------TWVSRVTNSVGSGSVRRDFFVAKR 244
Query: 51 LDRFLLSDNCYSTWPNCIQIALQSGLSDHVHMVLRVD---EENWGPRPLRMLKCWANFPG 107
LDR L + W L SDH + L++ +N RP R W + PG
Sbjct: 245 LDRVLCCSHARLKWQEATLTHLPFLASDHAPLYLQLALGVGKNRFRRPFRFEAAWLSHPG 304
Query: 108 Y 108
+
Sbjct: 305 F 305
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 31.2 bits (69), Expect = 0.088
Identities = 19/58 (32%), Positives = 29/58 (49%), Gaps = 2/58 (3%)
Query: 35 GRLFTW--YRGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGLSDHVHMVLRVDEEN 90
G FTW R D + KLDR + +S +PN Q L+ SDH ++++ +N
Sbjct: 154 GNSFTWGGNRNDQWVQCKLDRCFGNPAWFSIFPNAHQWFLEKFGSDHRPVLVKFTNDN 211
>At2g05550 putative non-LTR retroelement reverse transcriptase
Length = 977
Score = 31.2 bits (69), Expect = 0.088
Identities = 22/102 (21%), Positives = 42/102 (40%), Gaps = 6/102 (5%)
Query: 19 FNKFIDDSFLVDFPICGRLFTWY--RGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGL 76
F + I L D G L+TW + + KLDR +++D ++ + G
Sbjct: 375 FQQMIRSCDLSDMASHGPLYTWCNKKEQDLILKKLDRVMVNDIWAQSFSQAYNVFEAGGC 434
Query: 77 SDHVHMVLRVDE----ENWGPRPLRMLKCWANFPGYALFVRE 114
SDH+ + +++ GP+P + + + V+E
Sbjct: 435 SDHLRCRIMLNDAAERSVKGPKPFKFVNAIIEMEDFKPMVKE 476
>At1g31100 hypothetical protein
Length = 1090
Score = 31.2 bits (69), Expect = 0.088
Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 2/64 (3%)
Query: 18 IFNKFIDDSFLVDFPICGRLFTWYRGDGYS--MSKLDRFLLSDNCYSTWPNCIQIALQSG 75
+F + ++ L D G FTW+ KLDR L++++ S +P+ + +
Sbjct: 79 VFRDCLFEAELCDLVFKGNTFTWWNKSATRPVAKKLDRILVNESWCSRFPSAYAVFGEPD 138
Query: 76 LSDH 79
SDH
Sbjct: 139 FSDH 142
>At2g45230 putative non-LTR retroelement reverse transcriptase
Length = 1374
Score = 30.8 bits (68), Expect = 0.11
Identities = 22/89 (24%), Positives = 36/89 (39%), Gaps = 10/89 (11%)
Query: 19 FNKFIDDSFLVDFPICGRLFTWY--RGDGYSMSKLDRFLLSDNCYSTWPNCIQIALQSGL 76
F + ++ L + G F+WY R D +LDR + + +P LQ
Sbjct: 160 FRQMLNSCGLWEVNHSGYQFSWYGNRNDELVQCRLDRTVANQAWMELFPQAKATYLQKIC 219
Query: 77 SDHVHMVLRVDEENWGPRPLRMLKCWANF 105
SDH ++ + +NW + WA F
Sbjct: 220 SDHSPLINNLVGDNW--------RKWAGF 240
>At1g78500 Putative Oxidosqualene Cyclase
Length = 767
Score = 30.4 bits (67), Expect = 0.15
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 7/58 (12%)
Query: 55 LLSDNCYSTWPNCIQIALQSGLSDHVHMVLRVDEEN------WGPRPLRMLKCWANFP 106
+LS+N + WP C ++ Q L + ++ DE + P+P ML CW P
Sbjct: 332 ILSENILNRWP-CNKLIRQKALRTTMELLHYQDEASRYFTGGCVPKPFHMLACWVEDP 388
>At1g25430 hypothetical protein
Length = 1213
Score = 29.6 bits (65), Expect = 0.25
Identities = 18/63 (28%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Query: 28 LVDFPICGRLFTWYRGDGYS--MSKLDRFLLSDNCYSTWPNCIQIALQSGLSDHVHMVLR 85
L D G FTW+ + K+DR L++D+ + +P+ + I SDHV +
Sbjct: 178 LSDLRYKGNTFTWWNKSHTTPVAKKIDRILVNDSWNALFPSSLGIFGSLDFSDHVSCGVV 237
Query: 86 VDE 88
++E
Sbjct: 238 LEE 240
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.327 0.142 0.481
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,829,201
Number of Sequences: 26719
Number of extensions: 111865
Number of successful extensions: 274
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 253
Number of HSP's gapped (non-prelim): 29
length of query: 114
length of database: 11,318,596
effective HSP length: 90
effective length of query: 24
effective length of database: 8,913,886
effective search space: 213933264
effective search space used: 213933264
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC149307.10