BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149205.3 + phase: 0 /pseudo
(523 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g45230 putative non-LTR retroelement reverse transcriptase 38 0.016
At3g25720 hypothetical protein 36 0.045
At4g09710 RNA-directed DNA polymerase -like protein 36 0.059
At3g45550 putative protein 36 0.059
At2g17910 putative non-LTR retroelement reverse transcriptase 36 0.059
At3g32110 non-LTR reverse transcriptase, putative 35 0.10
At2g15510 putative non-LTR retroelement reverse transcriptase 35 0.10
At3g26855 putative protein 34 0.17
At2g31520 putative non-LTR retroelement reverse transcriptase 33 0.29
At2g41580 putative non-LTR retroelement reverse transcriptase 33 0.39
At1g43730 hypothetical protein 33 0.39
At2g07730 putative non-LTR retroelement reverse transcriptase 33 0.50
At1g29650 reverse transcriptase, putative 33 0.50
At2g16680 putative non-LTR retroelement reverse transcriptase 32 0.86
At2g05200 putative non-LTR retroelement reverse transcriptase 32 0.86
At3g09510 putative non-LTR reverse transcriptase 31 1.5
At2g31080 putative non-LTR retroelement reverse transcriptase 31 1.5
At2g25550 putative non-LTR retroelement reverse transcriptase 31 1.5
At1g33710 hypothetical protein 31 1.5
At2g17610 putative non-LTR retroelement reverse transcriptase 31 1.9
>At2g45230 putative non-LTR retroelement reverse transcriptase
Length = 1374
Score = 37.7 bits (86), Expect = 0.016
Identities = 29/105 (27%), Positives = 44/105 (41%), Gaps = 12/105 (11%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTS--------QE*--PHVHQNMELIWHKQVPLKVSILA 458
DR+ W + Y+V+ Y ++T QE P + + IW VP K+
Sbjct: 1008 DRFTWEYSRSGHYSVKSGYWVMTEIINQRNNPQEVLQPSLDPIFQQIWKLDVPPKIHHFL 1067
Query: 459 LRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
R + + L SNLA R + ++ CV H E H+ CP
Sbjct: 1068 WRCVNNCLSVASNLAYRHL--AREKSCVRCPSHGETVNHLLFKCP 1110
>At3g25720 hypothetical protein
Length = 282
Score = 36.2 bits (82), Expect = 0.045
Identities = 44/158 (27%), Positives = 66/158 (40%), Gaps = 28/158 (17%)
Query: 369 GGGGWWGAWQWRRHLWVWEEDLVEECGDLLLTV--SLQELETDRWMW-----LSDQIRGY 421
GGG W W HL + GD ++ + QEL +D W W L +
Sbjct: 50 GGGSLWVDWHRYHHL--------QGLGDASVSKFWTSQELVSDSWNWKCLLRLRPLAERF 101
Query: 422 TVRGVYDMLTSQE*PHVHQNMELIWHKQVPLKVSILALRLLRDRLPTKSNLAD--RGITF 479
+ + LT++ N W PL + I A R R R+P ++++AD +GI++
Sbjct: 102 LRCNIGNGLTAR---FWTDN----WTPFGPLLIHIGAARPNRLRIPIEASVADATQGISW 154
Query: 480 ---VEDRICVTGCGHVEDTTHMFLSCPIFGALLSMVQT 514
V C+ C E H+ L C AL S VQ+
Sbjct: 155 GLPVSPLCCLCSCA-PETRDHLLLGCSFSKALWSSVQS 191
>At4g09710 RNA-directed DNA polymerase -like protein
Length = 1274
Score = 35.8 bits (81), Expect = 0.059
Identities = 31/133 (23%), Positives = 52/133 (38%), Gaps = 13/133 (9%)
Query: 374 WGAWQWRRHLWVWEEDLVEECGDLLLTVSLQELETDRWMWLSDQIRGYTVRGVYDMLTSQ 433
W R+HL +E+ + + +T++ L+ D +WL + YT + Y +
Sbjct: 907 WNVEAIRKHLPQYEDQIRK------ITINALPLQ-DSLVWLPVKSGEYTTKTGYALAKLN 959
Query: 434 E*PHVHQNMEL---IWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVTGCG 490
P + IW KV + ++ LP L+ R I E + CG
Sbjct: 960 SFPASQLDFNWQKNIWKIHTSPKVKHFLWKAMKGALPVGEALSRRNI---EAEVTCKRCG 1016
Query: 491 HVEDTTHMFLSCP 503
E + H+ L CP
Sbjct: 1017 QTESSLHLMLLCP 1029
>At3g45550 putative protein
Length = 851
Score = 35.8 bits (81), Expect = 0.059
Identities = 29/106 (27%), Positives = 43/106 (40%), Gaps = 11/106 (10%)
Query: 407 ETDRWMWLSDQIRGYTVRGVYDMLT--------SQE*PHVHQNMEL-IWHKQVPLKVSIL 457
+TDR +W + YTVR Y + T + PH +++ IW+ + K+
Sbjct: 620 KTDRLIWSYNSTGDYTVRSGYWLSTHDPSNTIPTMAKPHGSVDLKTKIWNLPIMPKLKHF 679
Query: 458 ALRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
R+L LPT L RG+ D C E H +CP
Sbjct: 680 LWRILSKALPTTDRLTTRGMRI--DPGCPRCRRENESINHALFTCP 723
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 35.8 bits (81), Expect = 0.059
Identities = 26/106 (24%), Positives = 41/106 (38%), Gaps = 12/106 (11%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQE*----------PHVHQNMELIWHKQVPLKVSILA 458
D + WL Y+V+ Y+ L+ Q P V+ + IW+ K+ I
Sbjct: 978 DSFCWLHSHNGLYSVKTGYEFLSKQVHHRLYQEAKVKPSVNSLFDKIWNLHTAPKIRIFL 1037
Query: 459 LRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCPI 504
+ L +P + L RGI D C+ E H+ CP+
Sbjct: 1038 WKALHGAIPVEDRLRTRGIR--SDDGCLMCDTENETINHILFECPL 1081
>At3g32110 non-LTR reverse transcriptase, putative
Length = 1911
Score = 35.0 bits (79), Expect = 0.10
Identities = 24/104 (23%), Positives = 42/104 (40%), Gaps = 8/104 (7%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQE*PHVHQNME----LIWHKQVPLKVSILALRLLRD 464
DR W +TV+ + MLT+ + P Q+M +W Q P +V + ++
Sbjct: 1544 DRLAWGMTSDGRFTVKSAFAMLTNDDSP--RQDMSSLYGRVWKVQAPERVRVFLWLVVNQ 1601
Query: 465 RLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCPIFGAL 508
+ T S R + + +C G +E H+ CP +
Sbjct: 1602 AIMTNSERKRRHL--CDSDVCQVCRGGIESILHVLRDCPAMSGI 1643
>At2g15510 putative non-LTR retroelement reverse transcriptase
Length = 1138
Score = 35.0 bits (79), Expect = 0.10
Identities = 31/132 (23%), Positives = 55/132 (41%), Gaps = 17/132 (12%)
Query: 386 WEEDLVEEC---GDLLLTVSLQELET--DRWMWLSDQIRGYTVRGVY---------DMLT 431
W+ DL+EE D+ L + D W+WL + Y+V+ Y D++
Sbjct: 748 WKRDLLEELFYPTDVELITKRNPVVNMDDFWVWLHAKTGEYSVKSGYWLAFQSNKLDLIQ 807
Query: 432 SQE*-PHVHQNMELIWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVTGCG 490
P + E +W + K+ + R+L LP + RG++ ++ R + G
Sbjct: 808 QANLLPSTNGLKEQVWSTKSSPKIKMFLWRILSAALPVADQIIRRGMS-IDPRCQICG-D 865
Query: 491 HVEDTTHMFLSC 502
E T H+ +C
Sbjct: 866 EGESTNHVLFTC 877
>At3g26855 putative protein
Length = 163
Score = 34.3 bits (77), Expect = 0.17
Identities = 18/59 (30%), Positives = 29/59 (48%), Gaps = 3/59 (5%)
Query: 445 IWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
IW ++ K+ +L + L + LP + L R I+ + C T C E TH+ +CP
Sbjct: 9 IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISI--EPFC-TRCRDFETITHILFNCP 64
>At2g31520 putative non-LTR retroelement reverse transcriptase
Length = 1524
Score = 33.5 bits (75), Expect = 0.29
Identities = 28/104 (26%), Positives = 42/104 (39%), Gaps = 11/104 (10%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQE*PHV------HQNMEL---IWHKQVPLKVSILAL 459
D+ +W + YTVR Y +LT ++ H +++L IW+ + K+
Sbjct: 1157 DKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLW 1216
Query: 460 RLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
R L L T L RG+ D IC E H +CP
Sbjct: 1217 RALSQALATTERLTTRGMRI--DPICPRCHRENESINHALFTCP 1258
>At2g41580 putative non-LTR retroelement reverse transcriptase
Length = 1094
Score = 33.1 bits (74), Expect = 0.39
Identities = 25/110 (22%), Positives = 43/110 (38%), Gaps = 20/110 (18%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQ----------E*PHVHQNMELIWHKQVPLKVSILA 458
D + W YTV+ YD+ + Q E P ++ IW+ K+ +
Sbjct: 729 DSFCWFGTNHGLYTVKSEYDLCSRQVHKQMFKEAEEQPSLNPLFGKIWNLNSAPKIKVFL 788
Query: 459 LRLLRDRLPTKSNLADRGITFVEDRICVTGCGHV----EDTTHMFLSCPI 504
++L+ + + L RG+ +ED GC E H+ CP+
Sbjct: 789 WKVLKGAVAVEDRLRTRGV-LIED-----GCSMCPEKNETLNHILFQCPL 832
>At1g43730 hypothetical protein
Length = 320
Score = 33.1 bits (74), Expect = 0.39
Identities = 18/72 (25%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
Query: 437 HVHQNMELIWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTT 496
H+ + +W K K + + + +RL T+ L G++ +C+ H E
Sbjct: 147 HIVPWYKAVWFKNHVPKHAFICWVVAWNRLHTRDRLRSWGLSI--PAVCLLCNSHDESRA 204
Query: 497 HMFLSCPIFGAL 508
H+F CP GA+
Sbjct: 205 HLFFECPFCGAV 216
>At2g07730 putative non-LTR retroelement reverse transcriptase
Length = 970
Score = 32.7 bits (73), Expect = 0.50
Identities = 27/122 (22%), Positives = 49/122 (40%), Gaps = 7/122 (5%)
Query: 384 WVWEEDLVEECGDLLLTVSLQELETDRWMWLSDQIRGYTVRGVYDML--TSQE*PHVHQN 441
W+ +E L + ++S L +D W Q +TVR Y++L ++E P +
Sbjct: 587 WLLQEQLSAVAKE---SISADALLSDELSWKGTQNGDFTVRSAYELLKPEAEERPLIGSF 643
Query: 442 MELIWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLS 501
++ IW P +V + + + T R ++ + C G E H+
Sbjct: 644 LKQIWKLVAPERVRVFIWLVSHMVIMTNVERVRRHLSDI--ATCSVCNGADESILHVLRD 701
Query: 502 CP 503
CP
Sbjct: 702 CP 703
>At1g29650 reverse transcriptase, putative
Length = 1557
Score = 32.7 bits (73), Expect = 0.50
Identities = 30/138 (21%), Positives = 50/138 (35%), Gaps = 25/138 (18%)
Query: 386 WEEDLVEECGDLLLTVSLQEL--------ETDRWMWLSDQIRGYTVRGVYDMLTSQE*PH 437
W DL++ DL V + + D W+WL + Y+V+ Y ++ P
Sbjct: 1246 WNRDLLQ---DLFFQVDIDLICKKKPVVDMDDFWVWLHSKSGEYSVKSGYWLVFQSNKPE 1302
Query: 438 V----------HQNMELIWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVT 487
+ + E IW K+ + R+L LP + RG+ C
Sbjct: 1303 LLFKACRQPSTNGLKEKIWSTSTFPKIKLFLWRILSAALPVADQILRRGMNVDP---CCQ 1359
Query: 488 GCGHV-EDTTHMFLSCPI 504
CG E H+ +C +
Sbjct: 1360 ICGQEGESINHVLFTCSV 1377
>At2g16680 putative non-LTR retroelement reverse transcriptase
Length = 1319
Score = 32.0 bits (71), Expect = 0.86
Identities = 24/117 (20%), Positives = 45/117 (37%), Gaps = 14/117 (11%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQE*----------PHVHQNMELIWHKQVPLKVSILA 458
D + W YTV+ Y+ + + P V+ + +W + K+ +
Sbjct: 952 DSYCWAGTNNGLYTVKSGYERSSRETFKNLFKEADVYPSVNPLFDKVWSLETVPKIKVFM 1011
Query: 459 LRLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCPIFGAL--LSMVQ 513
+ L+ L + L RGI + C+ +E H+ CP + LS++Q
Sbjct: 1012 WKALKGALAVEDRLRSRGIRTADG--CLFCKEEIETINHLLFQCPFARQVWALSLIQ 1066
>At2g05200 putative non-LTR retroelement reverse transcriptase
Length = 1229
Score = 32.0 bits (71), Expect = 0.86
Identities = 27/97 (27%), Positives = 38/97 (38%), Gaps = 6/97 (6%)
Query: 409 DRWMWLSDQIRGYTVRGVYDM--LTSQE*PHVHQNMEL-IWHKQVPLKVSILALRLLRDR 465
D+ WL + YT R Y + + S P N + +W Q K+ L + +
Sbjct: 895 DKLAWLPVKSGQYTSRSGYGIASVASIPIPQTQFNWQSNLWKLQTLPKIKHLMWKAAMEA 954
Query: 466 LPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSC 502
LP L R I+ CG E TTH+F C
Sbjct: 955 LPVGIQLVRRHIS---PSAACHRCGAPESTTHLFFHC 988
>At3g09510 putative non-LTR reverse transcriptase
Length = 484
Score = 31.2 bits (69), Expect = 1.5
Identities = 27/104 (25%), Positives = 41/104 (38%), Gaps = 11/104 (10%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQE*PHV------HQNMEL---IWHKQVPLKVSILAL 459
D+ +W + YTVR Y +LT ++ H +++L IW+ + K+
Sbjct: 117 DKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLW 176
Query: 460 RLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
R L L T L RG+ D C E H +CP
Sbjct: 177 RALSQALATTERLTTRGMRI--DPSCPRCHRENESINHALFTCP 218
>At2g31080 putative non-LTR retroelement reverse transcriptase
Length = 1231
Score = 31.2 bits (69), Expect = 1.5
Identities = 23/97 (23%), Positives = 38/97 (38%), Gaps = 4/97 (4%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQ--E*PHVHQNMELIWHKQVPLKVSILALRLLRDRL 466
D W Q +TVR Y +L + P++ IW P +V + + ++ +
Sbjct: 868 DEISWKGTQDGAFTVRSAYSLLQGDVGDRPNMGSFFNRIWKLITPERVRVFIWLVSQNVI 927
Query: 467 PTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
T R ++ E+ IC G E H+ CP
Sbjct: 928 MTNVERVRRHLS--ENAICSVCNGAEETILHVLRDCP 962
>At2g25550 putative non-LTR retroelement reverse transcriptase
Length = 1750
Score = 31.2 bits (69), Expect = 1.5
Identities = 27/104 (25%), Positives = 41/104 (38%), Gaps = 11/104 (10%)
Query: 409 DRWMWLSDQIRGYTVRGVYDMLTSQE*PHV------HQNMEL---IWHKQVPLKVSILAL 459
D+ +W + YTVR Y +LT ++ H +++L IW+ + K+
Sbjct: 1383 DKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLW 1442
Query: 460 RLLRDRLPTKSNLADRGITFVEDRICVTGCGHVEDTTHMFLSCP 503
R L L T L RG+ D C E H +CP
Sbjct: 1443 RALSQALATTERLTTRGMRI--DPSCPRCHRENESINHALFTCP 1484
>At1g33710 hypothetical protein
Length = 210
Score = 31.2 bits (69), Expect = 1.5
Identities = 18/43 (41%), Positives = 23/43 (52%), Gaps = 10/43 (23%)
Query: 464 DRLPTKSNLADRGITFVEDRICVTGCG----HVEDTTHMFLSC 502
DRLPTK+ LA G+ T CG +ED H+FL+C
Sbjct: 71 DRLPTKTRLASWGMQL------QTTCGLCSLDIEDRDHLFLTC 107
>At2g17610 putative non-LTR retroelement reverse transcriptase
Length = 773
Score = 30.8 bits (68), Expect = 1.9
Identities = 19/61 (31%), Positives = 26/61 (42%), Gaps = 4/61 (6%)
Query: 445 IWHKQVPLKVSILALRLLRDRLPTKSNLADRGITFVEDRICVTGCGHV-EDTTHMFLSCP 503
IW + P K+ R + LPT NL R + + D C CG ED H+ C
Sbjct: 501 IWKQNAPPKIKHFWWRSAHNALPTAGNLKRRRL--ITDDTC-QRCGEASEDVNHLLFQCR 557
Query: 504 I 504
+
Sbjct: 558 V 558
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.364 0.164 0.688
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,026,441
Number of Sequences: 26719
Number of extensions: 370408
Number of successful extensions: 2118
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 2098
Number of HSP's gapped (non-prelim): 36
length of query: 523
length of database: 11,318,596
effective HSP length: 104
effective length of query: 419
effective length of database: 8,539,820
effective search space: 3578184580
effective search space used: 3578184580
T: 11
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.5 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149205.3