BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149197.7 - phase: 0
(218 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g64600 unknown protein 317 3e-87
At1g52160 unknown protein 32 0.20
At2g21380 putative kinesin heavy chain 32 0.34
At3g01320 unknown protein 30 0.75
At4g37630 putative protein 30 1.3
At5g61460 SMC-like protein (MIM) 29 2.2
At5g39865 unknown protein 29 2.2
At1g24190 hypothetical protein 28 3.7
At4g34310 hypothetical protein 28 4.9
At5g07580 transcription factor-like protein (emb|CAB87947.1) 27 6.4
At3g60840 putative protein 27 6.4
At3g56270 unknown protein 27 6.4
At3g16260 unknown protein 27 6.4
At2g29590 unknown protein 27 6.4
At1g13060 proteasome epsilon chain precursor 27 6.4
At4g30240 unknown protein 27 8.3
At3g11990 unknown protein 27 8.3
At1g60610 unknown protein 27 8.3
At1g51600 putative zinc finger protein (At1g51600) 27 8.3
>At1g64600 unknown protein
Length = 537
Score = 317 bits (812), Expect = 3e-87
Identities = 151/210 (71%), Positives = 184/210 (86%), Gaps = 1/210 (0%)
Query: 7 RMLVEVSRRIDGLSPTKVLDFGAGTVSAFWALQEVWPKSLEKVNIIEPSQSMQRAGQRLI 66
R+L+E+ RR+ G +PT+VLDFGAGT S FWA++EVWPKS+EKVNI+EPSQSMQRAG+ LI
Sbjct: 122 RVLIEIRRRVPGFTPTRVLDFGAGTGSGFWAVKEVWPKSVEKVNIVEPSQSMQRAGRNLI 181
Query: 67 QGLKDLPLIHSYDSIQALSKDISK-SERGHDLVIASYVLGEIPSIKDRITILRQLWDQTQ 125
QGLKDLPLIH Y S+ AL+K+I+K SER HDLVIASYVLGEIPS+KDRIT++RQLWD T
Sbjct: 182 QGLKDLPLIHGYTSLLALNKEINKKSERKHDLVIASYVLGEIPSLKDRITVVRQLWDLTD 241
Query: 126 DVLVLVEPGTPHGSSIIAQMRSYILWMEERKHRKSSKKNNEVCKDLITEKAGAFVVAPCP 185
D+LVLVEPGTPHG++II+QMRS+ILWME+RK RK KK + K+++ K+GA +VAPCP
Sbjct: 242 DLLVLVEPGTPHGANIISQMRSHILWMEKRKLRKLEKKMKKDGKEVLDLKSGAHIVAPCP 301
Query: 186 HDGTCPLVKSGKYCHFVQRLERTSSQRAYK 215
HDG CPL +GKYCHFVQRL+RTSSQR+YK
Sbjct: 302 HDGKCPLENTGKYCHFVQRLQRTSSQRSYK 331
>At1g52160 unknown protein
Length = 890
Score = 32.3 bits (72), Expect = 0.20
Identities = 23/99 (23%), Positives = 45/99 (45%), Gaps = 4/99 (4%)
Query: 35 FWALQEVWPKSLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERG 94
F+ WP L +II+P+ S + + L + + L+ ++ + I +S
Sbjct: 417 FFPAPGFWPSQLTDNSIIDPTPSNKCSSSNLAESISAENLL-KFNLRPVAIRGIDRSCIP 475
Query: 95 HDLVIASYV---LGEIPSIKDRITILRQLWDQTQDVLVL 130
L + V L EIP IKD+ ++Q W++ + ++
Sbjct: 476 APLTSSEVVDELLSEIPEIKDKSEEIKQFWNKQHNKTII 514
>At2g21380 putative kinesin heavy chain
Length = 1058
Score = 31.6 bits (70), Expect = 0.34
Identities = 19/79 (24%), Positives = 41/79 (51%), Gaps = 7/79 (8%)
Query: 118 RQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERKHRK------SSKKNNEVCKDL 171
RQL + ++V+V TP ++A++++ + M+E++ + ++ N+ +CK +
Sbjct: 955 RQLVNGHEEVIVAKAEETPKEEPLVARLKARMQEMKEKEMKSQAAAAANADANSHICK-V 1013
Query: 172 ITEKAGAFVVAPCPHDGTC 190
E A ++ PC H C
Sbjct: 1014 CFESPTATILLPCRHFCLC 1032
>At3g01320 unknown protein
Length = 1324
Score = 30.4 bits (67), Expect = 0.75
Identities = 27/94 (28%), Positives = 38/94 (39%), Gaps = 13/94 (13%)
Query: 73 PLIHSYDSIQALSKDISKSERGHDLVIASYVLGEIPSIKDRITILRQLWDQTQDVLVLVE 132
P SY + LS K E GHD+V ++ +T L L + V V+
Sbjct: 1218 PTFASYMQTELLSVSSGKKEEGHDIV-----------LQRNLTGLYDLCKAMEGVEVV-- 1264
Query: 133 PGTPHGSSIIAQMRSYILWMEERKHRKSSKKNNE 166
G S + +Y+L E+ HRK KK E
Sbjct: 1265 NGLECKMSCSSYKIAYVLDTEDYFHRKKKKKKTE 1298
>At4g37630 putative protein
Length = 321
Score = 29.6 bits (65), Expect = 1.3
Identities = 31/114 (27%), Positives = 51/114 (44%), Gaps = 7/114 (6%)
Query: 48 KVNIIEP----SQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYV 103
K+N+I P + + + Q KDL L+ S DS+ AL+K+IS +E +V A
Sbjct: 169 KMNLITPFHYFNYFLAKISQDNHSVSKDLVLLRSSDSLLALTKEISFTEYRQFVVAAVTT 228
Query: 104 LGEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERKH 157
L S I + R ++ + + T + + + L +EERKH
Sbjct: 229 LLASSSTSSDIRLTR---EEIANKFGSISWWTSNENENVYLCYQRTLEIEERKH 279
>At5g61460 SMC-like protein (MIM)
Length = 1057
Score = 28.9 bits (63), Expect = 2.2
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 7/58 (12%)
Query: 154 ERKHRKSSKKNNEVCKDLITEKAGAFVVAPCPHDGTCPLVKSGKYCHFVQRLERTSSQ 211
+ K ++S +K +E+C + E G P DG+ P S + QRL R + Q
Sbjct: 818 KNKRKESDQKASEICPESEIESLG-------PWDGSTPEQLSAQITRMNQRLHRENQQ 868
>At5g39865 unknown protein
Length = 390
Score = 28.9 bits (63), Expect = 2.2
Identities = 23/98 (23%), Positives = 45/98 (45%), Gaps = 4/98 (4%)
Query: 3 EVGVRMLVEVSRRIDGLSPTKVLDFGAGTVSA-FWA--LQEVWPKSLEKVNIIEPSQSMQ 59
++G L+ + + G LD A + A W+ + E PK + K I+ P +
Sbjct: 82 QMGEETLISGNGFLHGDEEKMNLDLQAKVIEAKVWSSTINEKIPKIVAKTPIVTPPGEPE 141
Query: 60 RAGQ-RLIQGLKDLPLIHSYDSIQALSKDISKSERGHD 96
L++GL+D+ + S + +++ S D + + HD
Sbjct: 142 TINTWELMEGLEDVSPLRSPNHLRSFSFDFVRIQPSHD 179
>At1g24190 hypothetical protein
Length = 1263
Score = 28.1 bits (61), Expect = 3.7
Identities = 23/130 (17%), Positives = 54/130 (40%), Gaps = 10/130 (7%)
Query: 47 EKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDS--IQALSKDISKSERGHDLVIASYV- 103
E ++ + ++ + R+ + D+ ++ DS I + ++++ R H ++ +
Sbjct: 100 EAISFVNKIKTRFQGDDRVYKSFLDILNMYRRDSKSITEVYQEVAILFRDHSDLLVEFTH 159
Query: 104 -------LGEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERK 156
IPS+K + +D ++ P +G+ I Q R + E ++
Sbjct: 160 FLPDTSATASIPSVKTSVRERGVSLADKKDRIITPHPDHDYGTEHIDQDRERPIKKENKE 219
Query: 157 HRKSSKKNNE 166
H + + K NE
Sbjct: 220 HMRGTNKENE 229
>At4g34310 hypothetical protein
Length = 1074
Score = 27.7 bits (60), Expect = 4.9
Identities = 24/99 (24%), Positives = 37/99 (37%), Gaps = 16/99 (16%)
Query: 105 GEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERKHRKSSKKN 164
G+ S K I+ + + D++ L+ PG PH W K R+S KKN
Sbjct: 864 GDGGSSKPDISNMNSNCPRYGDMIFLINPGLPH-------------WKCHEKERQSGKKN 910
Query: 165 NEVCKD---LITEKAGAFVVAPCPHDGTCPLVKSGKYCH 200
+ +T+ G VV + SG + H
Sbjct: 911 ESSSEGEPANVTDTVGDHVVDASNLSSSIDPSSSGSHVH 949
>At5g07580 transcription factor-like protein (emb|CAB87947.1)
Length = 207
Score = 27.3 bits (59), Expect = 6.4
Identities = 20/84 (23%), Positives = 36/84 (42%), Gaps = 7/84 (8%)
Query: 26 DFGAGTVSAFWALQEVWPKSLEKVNIIEP----SQSMQRAGQRLIQGLKDLPLIHSYDSI 81
DF A VS W ++ PK +++P ++ +Q G+ P ++S S
Sbjct: 32 DFDASFVSGLWCIEPHVPKQEPDSPVLDPDSFVNEFLQVEGE---SSSSSSPELNSSSST 88
Query: 82 QALSKDISKSERGHDLVIASYVLG 105
+ + K+ER + V A + G
Sbjct: 89 YETDQSVKKAERFEEEVDARHYRG 112
>At3g60840 putative protein
Length = 648
Score = 27.3 bits (59), Expect = 6.4
Identities = 21/99 (21%), Positives = 45/99 (45%), Gaps = 13/99 (13%)
Query: 100 ASYVLGEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERKHRK 159
A ++ ++P + + +T W+ + L + G +++ + Y EE++H K
Sbjct: 391 ARLLVNKLPGMVEALTAKVTAWENERGNEFLYD-----GVRVLSMLGQYKTVWEEKEHEK 445
Query: 160 SSKKN-NEVCKDLITEKAGAFVVAPCPHDGTCPLVKSGK 197
+++ ++ LITE+ + P P+ KSGK
Sbjct: 446 QRQRDMKKLHGQLITEQEALYGSKPSPN-------KSGK 477
>At3g56270 unknown protein
Length = 446
Score = 27.3 bits (59), Expect = 6.4
Identities = 20/57 (35%), Positives = 31/57 (54%), Gaps = 5/57 (8%)
Query: 55 SQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVLGEIPSIK 111
+ M++AG L + + DL LIHSY A S ++ E L +AS + E+ S+K
Sbjct: 155 TMEMKQAGMNLCKTMDDLALIHSY----AESLNMKTKEEKDVLGVAS-LAEELNSLK 206
>At3g16260 unknown protein
Length = 942
Score = 27.3 bits (59), Expect = 6.4
Identities = 11/37 (29%), Positives = 21/37 (56%)
Query: 97 LVIASYVLGEIPSIKDRITILRQLWDQTQDVLVLVEP 133
L + +L EIP I + ++QLW+ + +++ EP
Sbjct: 519 LRVMDELLSEIPEISSKTEEIKQLWNGQHNKMMIEEP 555
>At2g29590 unknown protein
Length = 158
Score = 27.3 bits (59), Expect = 6.4
Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Query: 58 MQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVLGEIPSIKDRITIL 117
+ G L+ G + LP+ S D A +SK++ G +L I S +LGE K I ++
Sbjct: 79 VDEVGGALVHG-EGLPMSVSVDMSIAF---LSKAKLGEELEITSRLLGERGGYKGTIVVV 134
Query: 118 R 118
R
Sbjct: 135 R 135
>At1g13060 proteasome epsilon chain precursor
Length = 274
Score = 27.3 bits (59), Expect = 6.4
Identities = 22/100 (22%), Positives = 43/100 (43%), Gaps = 3/100 (3%)
Query: 71 DLPLIHSYDSIQALSKDISKSERGHDLVIASYVLGEIPSIKDRITILRQLWDQTQDVLVL 130
DLP +D Q +KD+ K +G + + G + + R ++ + Q+ ++
Sbjct: 34 DLPRTKEFDGFQKKAKDMLKHAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKIIE 93
Query: 131 VEP---GTPHGSSIIAQMRSYILWMEERKHRKSSKKNNEV 167
+ P GT G + Q L ++ R H ++K+ V
Sbjct: 94 INPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISV 133
>At4g30240 unknown protein
Length = 300
Score = 26.9 bits (58), Expect = 8.3
Identities = 9/25 (36%), Positives = 19/25 (76%)
Query: 147 SYILWMEERKHRKSSKKNNEVCKDL 171
+Y LW +E++ + S +++E+CK+L
Sbjct: 32 AYRLWFKEKRDGRVSVESDELCKEL 56
>At3g11990 unknown protein
Length = 172
Score = 26.9 bits (58), Expect = 8.3
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 6/50 (12%)
Query: 151 WMEERKHRKSSKKNNEVCKDLITEKAGAFVVAPCPHDGTCPLVKSGKYCH 200
++E + SSK NEV K+++ E + C D ++K GK CH
Sbjct: 82 FLEACSEKPSSKCGNEVFKNMLNET--MLITEECCRD----ILKMGKDCH 125
>At1g60610 unknown protein
Length = 340
Score = 26.9 bits (58), Expect = 8.3
Identities = 16/71 (22%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Query: 53 EPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISK--SERGHDLVIASYVLGEIPSI 110
E Q ++ ++ +G++D+ H + AL KD+SK E+ H++ + E+
Sbjct: 151 ELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNRELVDK 210
Query: 111 KDRITILRQLW 121
++ + Q W
Sbjct: 211 IKQVAVEAQNW 221
>At1g51600 putative zinc finger protein (At1g51600)
Length = 302
Score = 26.9 bits (58), Expect = 8.3
Identities = 13/31 (41%), Positives = 18/31 (57%)
Query: 133 PGTPHGSSIIAQMRSYILWMEERKHRKSSKK 163
PGTP SI ++ S + + E+RK R KK
Sbjct: 136 PGTPQRFSIPQRLASLVRFREKRKGRNFDKK 166
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,723,145
Number of Sequences: 26719
Number of extensions: 188911
Number of successful extensions: 553
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 545
Number of HSP's gapped (non-prelim): 19
length of query: 218
length of database: 11,318,596
effective HSP length: 95
effective length of query: 123
effective length of database: 8,780,291
effective search space: 1079975793
effective search space used: 1079975793
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC149197.7