BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149134.13 - phase: 0
(163 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g56370 unknown protein 65 2e-11
At5g38590 unknown protein 61 2e-10
At3g59210 unknown protein 60 5e-10
At2g04230 hypothetical protein 60 5e-10
At3g59180 putative protein 59 2e-09
At4g14096 F-box protein - like 58 2e-09
At5g62970 putative protein 58 3e-09
At3g59240 putative protein 57 6e-09
At1g66290 hypothetical protein 56 7e-09
At5g22660 unknown protein 55 2e-08
At5g56560 putative protein 54 3e-08
At4g10400 putative protein 54 3e-08
At5g56380 unknown protein 54 5e-08
At4g09920 hypothetical protein (probably remnant) 53 6e-08
At3g59170 putative protein 53 6e-08
At4g10420 putative protein 53 8e-08
At5g56410 putative protein 52 1e-07
At5g56700 putative protein 52 2e-07
At5g25860 putative protein 52 2e-07
At4g00320 52 2e-07
>At5g56370 unknown protein
Length = 421
Score = 65.1 bits (157), Expect = 2e-11
Identities = 41/107 (38%), Positives = 61/107 (56%), Gaps = 2/107 (1%)
Query: 9 PIL-SFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSI 67
P+L S HL+ DI ++ IA+++G+ L+ D+ H LP +F+ TL++
Sbjct: 72 PVLESLHLKLGRQCSEVDIGFWVRIAVEKGLCELDFDYEHYKTEPCRLPQSLFTCGTLTV 131
Query: 68 LKLKQITLNEVPF-VNLPSLKALYLDVVTFTYYELILKLLSGCPILQ 113
LKLK ++L +V F V LK L+L+ V F E KLLS CPIL+
Sbjct: 132 LKLKNVSLKDVQFPVCFKLLKTLHLEYVIFLDKETPQKLLSSCPILE 178
>At5g38590 unknown protein
Length = 410
Score = 61.2 bits (147), Expect = 2e-10
Identities = 42/115 (36%), Positives = 63/115 (54%), Gaps = 11/115 (9%)
Query: 20 DYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKLKQITLNEVP 79
D DI ++ IA+ R V +L+ID H ++ S F+ K+L LKL+ +TL ++P
Sbjct: 86 DIKPEDIRRWIEIAVSRHVHDLDID--HFSENENIFLSSFFACKSLVTLKLRSVTLRDIP 143
Query: 80 -FVNLPSLKALYLDVVTFTYYELILKLLSGCPILQ--------YLGTNNLVVELP 125
V LPSLK L LD V+F + + +LLS CP+L+ Y T L + +P
Sbjct: 144 SMVCLPSLKTLLLDNVSFVEGKSLQELLSICPVLEDLSVYCDDYENTKELTIVVP 198
>At3g59210 unknown protein
Length = 484
Score = 60.1 bits (144), Expect = 5e-10
Identities = 44/114 (38%), Positives = 62/114 (53%), Gaps = 5/114 (4%)
Query: 10 ILSFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILK 69
I F L+C + + D++ ++ ++ GV +L++ S SL LPS VF+SKTL LK
Sbjct: 87 INKFSLKCGDGIEDEDVFPWILNTLRHGVSDLSLHVSPSLV--YWLPSKVFASKTLVRLK 144
Query: 70 LKQITLNEVPFVN--LPSLKALYLDVVTFTYYEL-ILKLLSGCPILQYLGTNNL 120
+ V N LP LK L LD V F ++ KLLSGCP+L+ L NL
Sbjct: 145 IGPKDGPRVKLRNVCLPKLKTLNLDSVVFEEGKIGFAKLLSGCPVLEELSLLNL 198
>At2g04230 hypothetical protein
Length = 454
Score = 60.1 bits (144), Expect = 5e-10
Identities = 46/110 (41%), Positives = 59/110 (52%), Gaps = 3/110 (2%)
Query: 9 PIL-SFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFS-SKTLS 66
P+L S HL DC + ++ A RGV L +D + +TLPS +FS + +L
Sbjct: 80 PVLESLHLSFEGRTDCLHVGIWIATAFARGVRKLVLDSFYQEDQTVTLPSVLFSYNDSLE 139
Query: 67 ILKLK-QITLNEVPFVNLPSLKALYLDVVTFTYYELILKLLSGCPILQYL 115
ILKLK I L+ V L SL+ LYLD V F E + LL GCP LQ L
Sbjct: 140 ILKLKCAIDLDFPSRVCLKSLRKLYLDQVHFKDEESVCNLLCGCPSLQDL 189
>At3g59180 putative protein
Length = 475
Score = 58.5 bits (140), Expect = 2e-09
Identities = 53/150 (35%), Positives = 77/150 (51%), Gaps = 16/150 (10%)
Query: 9 PILSFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSIL 68
P+ F L+C D + ++ + RGV L++D + S S LPS VF +KTL L
Sbjct: 53 PLNRFSLKCKVRCDSACVDGWIINVLDRGV--LDLDLNISSESDYKLPSEVFLNKTLVRL 110
Query: 69 KL--KQITLNEVPFVNLPSLKALYLDVVTFTYYE----LILKLLSGCPILQYLGTNNLVV 122
KL K + +V V LP LK LYL F +E KL+SGCP+L+ L +++
Sbjct: 111 KLGGKDEFIIDVEEVFLPKLKTLYLH--GFVCFEDSGVACAKLISGCPVLEEL----VMI 164
Query: 123 ELPYSERPVISLSN--LIRANICDIHIEFD 150
L + V S+SN L R IC I+++
Sbjct: 165 NLMWGLMDVCSVSNPTLKRVTICSEIIDYN 194
>At4g14096 F-box protein - like
Length = 468
Score = 58.2 bits (139), Expect = 2e-09
Identities = 41/108 (37%), Positives = 58/108 (52%), Gaps = 3/108 (2%)
Query: 9 PILSFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSIL 68
P+ F L+ + + I ++ ++RGV +L D + ++ PS +F SKTL L
Sbjct: 85 PLHKFSLKIGDGVEPDRIIPWINNVLERGVSDL--DLHVYMETEFVFPSEMFLSKTLVRL 142
Query: 69 KLKQITLNEVPFVNLPSLKALYLDVVTFTYYEL-ILKLLSGCPILQYL 115
KL L E V LP LK LY+D F Y + + KLLSGCPIL+ L
Sbjct: 143 KLMLYPLLEFEDVYLPKLKTLYIDSCYFEKYGIGLTKLLSGCPILEDL 190
>At5g62970 putative protein
Length = 449
Score = 57.8 bits (138), Expect = 3e-09
Identities = 39/101 (38%), Positives = 60/101 (58%), Gaps = 6/101 (5%)
Query: 25 DIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKLKQITLNEVPFV-NL 83
DI ++ IA+ R + L++DF LP +++ K+L LKL+ L +VP V +L
Sbjct: 116 DIKLWVEIAVSRCAQELSVDFFPKEKHNALLPRNLYTCKSLVTLKLRNNILVDVPHVFSL 175
Query: 84 PSLKALYLDVVTFTYYELILKLLSGCPILQYLGTNNLVVEL 124
PSLK L+L+ VT+ E + +LLS C +L+ +LVVEL
Sbjct: 176 PSLKILHLERVTYGDGESLQRLLSNCSVLE-----DLVVEL 211
>At3g59240 putative protein
Length = 504
Score = 56.6 bits (135), Expect = 6e-09
Identities = 39/114 (34%), Positives = 64/114 (55%), Gaps = 5/114 (4%)
Query: 10 ILSFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILK 69
++ F L C N D I+ ++ I+RGV +L D + S ++PS VF SK+L L+
Sbjct: 88 LVRFSLNCRNGIDRECIFRWISNVIERGVSDL--DLGGNFVSNRSMPSSVFVSKSLVKLR 145
Query: 70 LK--QITLNEVPFVNLPSLKALYLDVVTFTYYE-LILKLLSGCPILQYLGTNNL 120
++ T+ ++ V LP LK L L + F + +LKL+SGC +L+ L ++L
Sbjct: 146 IRTENCTIIDLEDVFLPKLKTLDLSSIWFRDGDTCLLKLISGCQVLEDLTMSDL 199
>At1g66290 hypothetical protein
Length = 453
Score = 56.2 bits (134), Expect = 7e-09
Identities = 35/107 (32%), Positives = 63/107 (58%), Gaps = 12/107 (11%)
Query: 13 FHLQCWNDYDCRDI------YNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLS 66
F L+C +D D D+ + ++Y+AI+R V+++++ + + + +++ ++L
Sbjct: 101 FKLRCGSDLD-GDVDLATCSWKWIYMAIKRKVQHIDVTWPG-----VRIYPEIYNCESLV 154
Query: 67 ILKLKQITLNEVPFVNLPSLKALYLDVVTFTYYELILKLLSGCPILQ 113
LKL ++TL + FV+LPSLK L LD V F L+SGCP+L+
Sbjct: 155 SLKLSEVTLTKPEFVSLPSLKVLVLDWVEFYNEFAFDMLMSGCPVLE 201
>At5g22660 unknown protein
Length = 315
Score = 54.7 bits (130), Expect = 2e-08
Identities = 42/140 (30%), Positives = 75/140 (53%), Gaps = 13/140 (9%)
Query: 11 LSFHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQM--TLPSFVFSSKTLSIL 68
+SF ++ D I ++ A++R +++L +D S M TLP V+ S++L L
Sbjct: 89 ISFQMEA---VDMWTIIPWIEDAVKRRIQHLEVD---SRIDHMIDTLPLTVYLSESLVSL 142
Query: 69 KLKQITLNEVPFVNLPSLKALYLDVVTFTYYELILKLLSGCPILQYLGTNNLVVE----- 123
+L + L+ FV+LP+LK ++L+ ++Y E + K +S CP+L+ L V E
Sbjct: 143 RLHLVMLHRFVFVSLPNLKVMHLEENIYSYAETMEKFISSCPVLEDLTVVRNVDEATEKV 202
Query: 124 LPYSERPVISLSNLIRANIC 143
L S + + SL +I ++ C
Sbjct: 203 LRVSSQSLNSLKLVIDSSKC 222
>At5g56560 putative protein
Length = 477
Score = 54.3 bits (129), Expect = 3e-08
Identities = 41/122 (33%), Positives = 62/122 (50%), Gaps = 9/122 (7%)
Query: 14 HLQCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQ-MTLPSFVFSSKTLSILKLKQ 72
HL+ Y DI ++ +A+ R V L ID LF + + LP + S TL L L
Sbjct: 83 HLKLNQKYSASDINFWVQVAVNRSVRELRID----LFGKTLELPCCLCSCITLKELTLHD 138
Query: 73 ITLNEVP-FVNLPSLKALYLDVVTFTYYELILKLLSGCPILQYL---GTNNLVVELPYSE 128
+ + VP + LPSLK L+L V F+ + +L CP+L+ L GT + V++P +
Sbjct: 139 LCIKVVPAWFRLPSLKTLHLLSVKFSSDGFVASILRICPVLERLVVDGTKGVNVKIPNMD 198
Query: 129 RP 130
P
Sbjct: 199 VP 200
>At4g10400 putative protein
Length = 326
Score = 54.3 bits (129), Expect = 3e-08
Identities = 37/95 (38%), Positives = 52/95 (53%), Gaps = 1/95 (1%)
Query: 25 DIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKLKQITLNEVP-FVNL 83
DI ++ +A+ R + L I SH Q LPS +++ K+L ILKL L +VP V L
Sbjct: 91 DIRMWVVVAVSRYIRELKIYSSHYGEKQNILPSSLYTCKSLVILKLDGGVLLDVPRMVCL 150
Query: 84 PSLKALYLDVVTFTYYELILKLLSGCPILQYLGTN 118
PSLK L L V + + +LL CP+L+ L N
Sbjct: 151 PSLKTLELKGVRYFKQGSLQRLLCNCPVLEDLVVN 185
>At5g56380 unknown protein
Length = 439
Score = 53.5 bits (127), Expect = 5e-08
Identities = 35/101 (34%), Positives = 61/101 (59%), Gaps = 5/101 (4%)
Query: 16 QCWNDYDCRDIYNFLYIAIQRGVENLNIDFSHSLF-SQMTLPSFVFSSKTLSILKLKQIT 74
QC YD DI ++ IA++RG+ L + ++ S + + +LP +++ +TL +LKLK+
Sbjct: 92 QC--SYD--DIAIWVGIAVKRGLMELKLKYTDSYYPKRSSLPRSLYTCETLVVLKLKKGY 147
Query: 75 LNEVPFVNLPSLKALYLDVVTFTYYELILKLLSGCPILQYL 115
L+ V L SLK L L + ++ +L+LL+ CP+L+ L
Sbjct: 148 LDVPDLVCLRSLKTLSLRDMNYSNASCLLRLLASCPVLEEL 188
>At4g09920 hypothetical protein (probably remnant)
Length = 414
Score = 53.1 bits (126), Expect = 6e-08
Identities = 36/92 (39%), Positives = 51/92 (55%), Gaps = 1/92 (1%)
Query: 25 DIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKLKQITLNEVP-FVNL 83
DI ++ +A+ R + L I SH Q LPS +++ K+L ILKL L +VP V L
Sbjct: 91 DIRMWVVVAVSRYIRELKIYSSHYGEKQNILPSSLYTCKSLVILKLDGGVLLDVPRMVCL 150
Query: 84 PSLKALYLDVVTFTYYELILKLLSGCPILQYL 115
PSLK L L V + + +LL CP+L+ L
Sbjct: 151 PSLKTLELKGVRYFKQGSLQRLLCNCPVLEDL 182
>At3g59170 putative protein
Length = 473
Score = 53.1 bits (126), Expect = 6e-08
Identities = 37/113 (32%), Positives = 63/113 (55%), Gaps = 6/113 (5%)
Query: 9 PILSFHLQCWNDYDCRD---IYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTL 65
P+ F L+ + +D D + +++ + RGV +L++ + ++ LPS +F S+TL
Sbjct: 105 PLDKFSLKMVDGHDPVDPDSVVPWIHKVLVRGVSDLHLVVDMNEWTSDPLPSRIFLSETL 164
Query: 66 --SILKLKQITLNEVPFVNLPSLKALYLDVVTFTYYEL-ILKLLSGCPILQYL 115
+K++ +V V+LP LK LYL V F ++ KLLSGCP+L+ L
Sbjct: 165 VKLTIKIRDGPFIDVKHVHLPKLKTLYLQSVMFDENDIGFRKLLSGCPVLEEL 217
>At4g10420 putative protein
Length = 239
Score = 52.8 bits (125), Expect = 8e-08
Identities = 36/91 (39%), Positives = 50/91 (54%), Gaps = 1/91 (1%)
Query: 29 FLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKLKQITLNEVP-FVNLPSLK 87
++ + + R V L I S Q LPS +++ K+L ILKL L +VP V LPSLK
Sbjct: 2 WVVVVLSRYVRELEIYSSCYQDKQNILPSSLYTCKSLVILKLNGGILMDVPRMVCLPSLK 61
Query: 88 ALYLDVVTFTYYELILKLLSGCPILQYLGTN 118
L L V + E + +LLS CP+L+ L N
Sbjct: 62 TLVLKGVNYFKQESLQRLLSNCPVLEDLVVN 92
>At5g56410 putative protein
Length = 503
Score = 52.0 bits (123), Expect = 1e-07
Identities = 42/117 (35%), Positives = 67/117 (56%), Gaps = 8/117 (6%)
Query: 10 ILSFHLQCWND-YDCRDIYNFLYIAIQRGVENLNIDFSHSLFSQ-MTLPSFVFSSKTLSI 67
I S L +N+ + I ++ IA+ R V+ L++ FS + LP+ +++ K+L
Sbjct: 93 IESLRLTIYNELFQPESINLWVEIAVSRCVKELSVRFSPFKGKRDALLPTTLYTCKSLVT 152
Query: 68 LKLKQITLNEVPFVN-LPSLKALYLDVVTFTYYELILKLLSGCPILQYLGTNNLVVE 123
LKL++ L +VP V LPSLK L+L VT+ E + +LLS C +L+ +LVVE
Sbjct: 153 LKLRENILVDVPHVFCLPSLKTLHLSHVTYADEESLQRLLSNCFVLE-----DLVVE 204
>At5g56700 putative protein
Length = 372
Score = 51.6 bits (122), Expect = 2e-07
Identities = 33/94 (35%), Positives = 58/94 (61%), Gaps = 3/94 (3%)
Query: 24 RDIYNFLYIAIQRGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKL--KQITLNEVPFV 81
+D+ +++ IA+ R V L+I H + ++LPS +++ K+L LKL K++ L+ V
Sbjct: 62 KDLKSWVRIAVSRCVRELSISL-HDTTAAVSLPSSLYTCKSLVTLKLYGKKVLLDVPRTV 120
Query: 82 NLPSLKALYLDVVTFTYYELILKLLSGCPILQYL 115
LPSLK L L+ + ++ + + LLS CP+L+ L
Sbjct: 121 FLPSLKTLQLERLRYSDEDSLRLLLSYCPVLEDL 154
>At5g25860 putative protein
Length = 448
Score = 51.6 bits (122), Expect = 2e-07
Identities = 39/118 (33%), Positives = 66/118 (55%), Gaps = 6/118 (5%)
Query: 24 RDIYNFLYIAIQRGVENLNIDFSHSLF-SQMTLPSFVFSSKTLSILKLK-QITLNEVPFV 81
+DI +L+ A++RGV +L++ F +Q P VF+ KTL L + ++ N++P+
Sbjct: 114 KDIDQWLHHAMERGVVDLDMRFKMGFTPTQNEWPCNVFTIKTLVKLTFRIEVGQNKMPYC 173
Query: 82 N---LPSLKALYLDVVTFTYYELILKLLSGCPILQYLGTNNLVVELPYSERPVISLSN 136
LP LK+L+L + F L +L GCPIL+ L ++L Y++ P S+S+
Sbjct: 174 PKGVLPVLKSLFLHAIWFACERLCHSMLPGCPILEELFLHDL-RGYRYNDSPNFSISH 230
>At4g00320
Length = 773
Score = 51.6 bits (122), Expect = 2e-07
Identities = 37/101 (36%), Positives = 57/101 (55%), Gaps = 7/101 (6%)
Query: 25 DIYNFLYIAIQ-RGVENLNIDFSHSLFSQMTLPSFVFSSKTLSILKLKQITLNEVPF-VN 82
DIY ++ +A+ V L++ LP ++ K++ ILKLK L +VP V
Sbjct: 94 DIYLWVSLAVHDSNVRELSLKLCTFAERPTKLPKSLYKCKSIVILKLKDEILVDVPRKVC 153
Query: 83 LPSLKALYLDVVTFTYYELILKLLSGCPILQYLGTNNLVVE 123
LPSLK L+L VT++ + + +LLS CP+L+ +LVVE
Sbjct: 154 LPSLKTLFLGRVTYSDEDSLHRLLSNCPVLE-----DLVVE 189
Score = 44.3 bits (103), Expect = 3e-05
Identities = 38/117 (32%), Positives = 61/117 (51%), Gaps = 10/117 (8%)
Query: 13 FHLQCWNDYDCRDIYNFLYIAIQRGVENLNIDF--SHSLFSQMT---LPSFVFSSKTLSI 67
F+++C + + ++ ++RGV ++++ S S T LPS +F SKTL
Sbjct: 366 FYVKC-EGVEQNSVLEWIPKVLKRGVLDIDLQIPSSRGFGSNSTFYPLPSEIFVSKTLVR 424
Query: 68 LKLK---QITLNEVPFVNLPSLKALYLDVVTFTYYELILKLLSGCPILQYLGTNNLV 121
LK++ +N V+LP LK L+LD V ++ KLLSGC L+ L NL+
Sbjct: 425 LKIQFQDGANVNVEGDVSLPMLKTLHLDYVKMDT-RMLQKLLSGCHTLEELLLMNLI 480
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.327 0.142 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,370,695
Number of Sequences: 26719
Number of extensions: 131458
Number of successful extensions: 632
Number of sequences better than 10.0: 165
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 63
Number of HSP's that attempted gapping in prelim test: 451
Number of HSP's gapped (non-prelim): 181
length of query: 163
length of database: 11,318,596
effective HSP length: 92
effective length of query: 71
effective length of database: 8,860,448
effective search space: 629091808
effective search space used: 629091808
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC149134.13