BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149134.10 + phase: 0
(478 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09030 unknown protein 572 e-163
At5g41330 putative protein 206 2e-53
At4g30940 unknown protein 99 7e-21
At2g24240 unknown protein 88 1e-17
At5g55000 FH protein interacting protein FIP2 (gb|AAF14550.1) 51 2e-06
At5g28530 far-red impaired response protein (FAR1) - like 33 0.45
At5g52250 unknown protein 32 0.59
At2g11620 pseudogene 32 0.77
At1g33680 single-strand nucleic acid-binding protein, putative 32 0.77
At3g04940 cysteine synthase (AtcysD1) 32 1.0
At1g13210 calcium-transporting ATPase like protein 31 1.3
At5g55660 putative protein 31 1.7
At2g01680 unknown protein 31 1.7
At4g38480 unknown protein 30 2.2
At3g02260 unknown protein 30 2.9
At4g35290 unknown protein 30 3.8
At4g02220 putative zinc finger protein 30 3.8
At5g65330 putative protein 29 5.0
At5g56880 unknown protein 29 5.0
At5g45850 putative protein 29 5.0
>At3g09030 unknown protein
Length = 460
Score = 572 bits (1474), Expect = e-163
Identities = 276/462 (59%), Positives = 355/462 (76%), Gaps = 13/462 (2%)
Query: 18 RIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSNDPNPVFIDRDPEIFSVFLSLLRTN 77
R+KLNVGG++FET STI+S PDSLL ALS S+ NPVFIDRDPEIF+V L+LLRT
Sbjct: 9 RVKLNVGGEIFETNASTIQSSCPDSLLAALSTSTSHGSNPVFIDRDPEIFAVILNLLRTG 68
Query: 78 HLPSTARR-FSKQELADEALFYGIDSHLRNATSPPPFSGIDASIVASVRPASEGLSSTFT 136
LP+ + FSKQEL DEA++YG++S LR A PPP G DAS+V+++ PA++G+ S T
Sbjct: 69 RLPANSSGVFSKQELLDEAMYYGVESLLRLAMLPPPLLGFDASLVSTIVPAADGVPSALT 128
Query: 137 A-AENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGTESDAGLHFY 195
A A + S+WIAHGGQ+S YDW+L H+ TVRTHL++I SICRVW E AA+G+ S +GLHFY
Sbjct: 129 ATAGDASLWIAHGGQISVYDWSLSHAGTVRTHLNDITSICRVWGEAAAIGSGSASGLHFY 188
Query: 196 NFSGSRHVGSVHWSDPSDPRIFKARVNAITASDNSVFASFDCPHRENCILEVDKAKLQIV 255
+ SG R++GS HW+DP DPRI KARV A+ S+ VFASFDC HREN +L++DK+ LQ+
Sbjct: 189 DLSGGRYIGSTHWTDPEDPRIHKARVAAVADSEGGVFASFDCLHRENSVLQIDKSTLQVA 248
Query: 256 SQLGRQSGNQAKNMVPGKLTFVPTTGVLVGSAVTGGAFGYSGYIRVWDPRSGEVVWETNE 315
+ +G+QSGN AK VP KL ++P G+LVGSAV G FG SGYIR+WDPRS +VWETNE
Sbjct: 249 AVIGQQSGNSAKTTVPEKLRWLPAKGLLVGSAVQRGVFGCSGYIRIWDPRSRNIVWETNE 308
Query: 316 PGAGRSSRFGDSFSAIDVDVEKLLLFKLCSKSGDLAMADMRRLGDDPWVYLKEKNPSLWT 375
PG+GRS+RFGD+ + +DVDVE +LFK+CSKSGDL MAD+R+LG+DPWVY+ ++NP W
Sbjct: 309 PGSGRSTRFGDALADMDVDVEDSILFKVCSKSGDLGMADIRKLGEDPWVYMSDENPGAW- 367
Query: 376 DGGDGSTSSVVNCYKGQVFVGRGGELEVWSRVKEVVECESERDRESDGEGVYRRNFVDKR 435
GDG SVV+CY+ QV RGG LEVWS VKE ++ G+ + RRNFVDK
Sbjct: 368 KAGDGGGYSVVHCYRKQVLAARGGALEVWSSVKE----------KTSGDPIRRRNFVDKE 417
Query: 436 EDSEKGVIVKIEGGGDRLFISREDVEGIEVWESSHYAGAISV 477
+DS++G+I KIE GGDRLF+SRE +EG+EVWE+S ++G +SV
Sbjct: 418 DDSKRGMISKIEAGGDRLFVSRECMEGVEVWETSSFSGVVSV 459
>At5g41330 putative protein
Length = 458
Score = 206 bits (524), Expect = 2e-53
Identities = 149/470 (31%), Positives = 243/470 (51%), Gaps = 40/470 (8%)
Query: 12 DSNVTDRIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSNDPNPVFIDRDPEIFSVFL 71
+SNV + +NVGG++F+TT T+ G DSLL L+ + F+DRDP++FSV L
Sbjct: 9 ESNV---VSINVGGRIFQTTKQTLSLAGTDSLLSQLATETTR-----FVDRDPDLFSVLL 60
Query: 72 SLLRTNHLPSTARRFSKQELADEALFYGIDS----HLRNATSPPPFSGIDASIV-ASVRP 126
+LRT +LP+ +R F ++L DE+ +YGI+S L N++ PF + I+ + R
Sbjct: 61 YILRTGNLPARSRAFDVRDLIDESRYYGIESFLIDSLSNSSQFEPFDLRRSRILQLNGRD 120
Query: 127 ASEGLSSTFTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGT 186
+ +S T G + +AHG +++S+DW+L +TV T+ ++S+ + P + A G
Sbjct: 121 SPSSISPTVI---GGGLHVAHGSKITSFDWSLRQKSTVLTNFSAVDSLLEISPGVLAAGA 177
Query: 187 ESDAGLHFYNFSGSRHV-GSVHWSDPSDPRIFKARVNAITASDNSVFASFDCPHR-ENCI 244
GL + V +++W + + + V AI +S +F SF+ R N I
Sbjct: 178 TDFPGLQIIDLDNGGFVRTTLNWENVTRS---SSTVQAIGSSHEFLFTSFESSRRNSNSI 234
Query: 245 LEVDKAKLQIVSQLGRQS--GNQAKNMVPG-KLTFVPTTGVLVGSAVTGGAFGYSGYIRV 301
+ D + L VS++ G + +P KL ++ + +L+ S G +G+IR
Sbjct: 235 MVYDLSSLLPVSEIDHCEIYGADIDSAIPSTKLKWIQSCNLLMVSGSHTSPSGVNGHIRF 294
Query: 302 WDPRSGEVVWETNEPGAGRSSRFGDSFSAIDVDVEKLLLFKLCSKSGDLAMADMRRLG-D 360
WD RS +VWE E D FS + V +FK+ SG++ AD+R LG
Sbjct: 295 WDVRSRNMVWEIKEA--------QDCFSDVTVSDNLSAVFKVGVTSGEVFYADLRSLGTK 346
Query: 361 DPWVYL-KEKNPSLWTDGGDGSTSSVVNCYKGQVFV-GRGGELEVWSRVKEVVECESERD 418
DPWV L +EK +L G GS + Y VF +G +E+WS V +
Sbjct: 347 DPWVCLGEEKKRNLNERRGVGSK---IESYGNHVFCSSKGSGIELWSEV--ITGLVGNAS 401
Query: 419 RESDGEGVYRRNFVDKREDSEKGVIVKIEGGGDRLFISREDVEGIEVWES 468
R+ E V+R+N + K DS + I + GG+R+F++R+D + ++VW+S
Sbjct: 402 RDVLEERVFRKNSLGKLADSGENKITGLAFGGNRMFVTRKDQQSVQVWQS 451
>At4g30940 unknown protein
Length = 441
Score = 98.6 bits (244), Expect = 7e-21
Identities = 60/160 (37%), Positives = 84/160 (52%), Gaps = 4/160 (2%)
Query: 17 DRIKLNVGGKLFETTLSTIRSGGPDSLLFAL--SNRFSNDPNPVFIDRDPEIFSVFLSLL 74
DRIK NVGG++FETT +T+ + G DS AL N + P +FIDR+P+ F+V L LL
Sbjct: 6 DRIKFNVGGRIFETTATTLANAGRDSFFGALFDENWNLSQPGDLFIDRNPDCFAVLLDLL 65
Query: 75 RTNHLPSTARRFSKQELADEALFYGIDSHLRNATSPPPFSGIDASIVASVRPASEGLSST 134
RT L + ++ L EA+FYG+ HLR A PF G + SV + G +
Sbjct: 66 RTGDL-NIPPNIPERLLHKEAMFYGLIDHLRTA-KWGPFDGNRLHLSRSVTGIAPGDGTA 123
Query: 135 FTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSI 174
A +G IAHG + +DW L T+ +N +
Sbjct: 124 IRAGPDGGCCIAHGSVVHVFDWMLEEHPTINLDYQRVNDV 163
>At2g24240 unknown protein
Length = 441
Score = 87.8 bits (216), Expect = 1e-17
Identities = 55/162 (33%), Positives = 84/162 (50%), Gaps = 6/162 (3%)
Query: 17 DRIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSN----DPNPVFIDRDPEIFSVFLS 72
DRIK NVGG+LFETT +T+ + G DS AL + N + + +F+DR+ + F+V L
Sbjct: 6 DRIKFNVGGRLFETTATTLANAGRDSFFGALFDDEWNLSPLEDSILFVDRNSDCFAVLLD 65
Query: 73 LLRTNHLPSTARRFSKQELADEALFYGIDSHLRNATSPPPFSGIDASIVASVRPASEGLS 132
LLRT L + ++ L EA FYG+ H+R A PF G + SV+ + G
Sbjct: 66 LLRTGDL-NVPANIPERLLHREASFYGLLDHVRTA-KWGPFDGNRLRLSDSVKGIAPGDG 123
Query: 133 STFTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSI 174
+ A +G +AHG + +DW L + + +N +
Sbjct: 124 TAIRAGPDGGCCVAHGSVVHVFDWMLEEHSPINLDYQRVNDV 165
>At5g55000 FH protein interacting protein FIP2 (gb|AAF14550.1)
Length = 298
Score = 50.8 bits (120), Expect = 2e-06
Identities = 36/93 (38%), Positives = 52/93 (55%), Gaps = 6/93 (6%)
Query: 11 TDSNVTDRIKLNVGGKLFETTLSTIRSGGPDSLLFAL-SNRFS----NDPNPVFIDRDPE 65
T SN++ ++LN+GGK F TT+ T+ PDS+L A+ S R + + VFIDRD +
Sbjct: 3 TSSNLSSMVRLNIGGKKFCTTIDTLTIREPDSMLAAMFSGRHAMCQESKKGYVFIDRDGK 62
Query: 66 IFSVFLSLLRTNHLPSTARRFSKQELADEALFY 98
F L+ LR +PS + EL EA +Y
Sbjct: 63 HFRHILNWLRDGVIPSLSDP-DCSELLREADYY 94
>At5g28530 far-red impaired response protein (FAR1) - like
Length = 700
Score = 32.7 bits (73), Expect = 0.45
Identities = 27/102 (26%), Positives = 43/102 (41%), Gaps = 13/102 (12%)
Query: 384 SVVNCYKGQVFVGRGGELEVWSRV--KEVVECESERDRESDGEGVYRRNFVDKRED-SEK 440
+V Y GQ+F E +S K R ES GVYRR+FV R ++
Sbjct: 66 TVFTPYVGQIFTTDDEAFEYYSTFARKSGFSIRKARSTESQNLGVYRRDFVCYRSGFNQP 125
Query: 441 GVIVKIE----------GGGDRLFISREDVEGIEVWESSHYA 472
+E G +L++++E V+G+ W S ++
Sbjct: 126 RKKANVEHPRERKSVRCGCDGKLYLTKEVVDGVSHWYVSQFS 167
>At5g52250 unknown protein
Length = 385
Score = 32.3 bits (72), Expect = 0.59
Identities = 13/37 (35%), Positives = 21/37 (56%)
Query: 283 LVGSAVTGGAFGYSGYIRVWDPRSGEVVWETNEPGAG 319
L ++ G + G +++WDPR+G + ET PG G
Sbjct: 176 LYNGSLIGASGSDDGTVQMWDPRNGGTLEETVRPGGG 212
>At2g11620 pseudogene
Length = 491
Score = 32.0 bits (71), Expect = 0.77
Identities = 26/91 (28%), Positives = 40/91 (43%), Gaps = 10/91 (10%)
Query: 76 TNHLPSTARRFSKQELADEALFYGIDSHLRNATSPP-------PFS--GIDASIVASVRP 126
TNH+P T + Q A++ F+ I +HL T PP P+S S ++V P
Sbjct: 71 TNHIPCTRGAPTVQPAANDQTFFSIGTHLGAPTVPPGANDHWFPYSDPACTNSGASAVPP 130
Query: 127 ASEGLSSTFT-AAENGSVWIAHGGQLSSYDW 156
A G +T N +V + G ++ W
Sbjct: 131 ADNGQWLPYTDPTYNYTVVYSTGAPAATAQW 161
>At1g33680 single-strand nucleic acid-binding protein, putative
Length = 407
Score = 32.0 bits (71), Expect = 0.77
Identities = 20/78 (25%), Positives = 36/78 (45%), Gaps = 7/78 (8%)
Query: 395 VGRGGELEVWSRVKEVVECESERDRESDGEGVYRR-------NFVDKREDSEKGVIVKIE 447
+G+GGE+ + +V + + RD E+D R + ++K E VI ++E
Sbjct: 247 IGKGGEMVRYLQVNSGAKIQIRRDAEADPSSALRPVEIIGTVSCIEKAEKLINAVIAEVE 306
Query: 448 GGGDRLFISREDVEGIEV 465
GG +R E +E+
Sbjct: 307 AGGVPALAARGVPEQMEI 324
>At3g04940 cysteine synthase (AtcysD1)
Length = 324
Score = 31.6 bits (70), Expect = 1.0
Identities = 29/132 (21%), Positives = 48/132 (35%), Gaps = 6/132 (4%)
Query: 269 MVPGKLTFVPTTG--VLVGSAVTGGAFGYSGYIRVWDPRSGEVVWETNEPGAGRSSRFGD 326
+ PGK T + T +G A G A GY + + P S + + G D
Sbjct: 66 ITPGKSTLIEATSGNTGIGLAFIGAAKGYKVVLTM--PSSMSLERKIILLALGAEVHLTD 123
Query: 327 SFSAIDVDVEKLLLFKLCSKSGDLAMADMRRLGDDPWVYLKEKNPSLWTDGGDGSTSSVV 386
+ ++K ++CSK+ D M + + +P + + P +W D V
Sbjct: 124 PSKGVQGIIDKAE--EICSKNPDSIMLEQFKNPSNPQTHYRTTGPEIWRDSAGEVDILVA 181
Query: 387 NCYKGQVFVGRG 398
G G G
Sbjct: 182 GVGTGGTLSGSG 193
>At1g13210 calcium-transporting ATPase like protein
Length = 1203
Score = 31.2 bits (69), Expect = 1.3
Identities = 15/47 (31%), Positives = 27/47 (56%), Gaps = 3/47 (6%)
Query: 401 LEVWSRVKEVVECESERDRESDGEGVYRRNFVDKREDSEKGVIVKIE 447
+E W R K+ +E + + + DG G++RR + D + G IV++E
Sbjct: 123 IEDWGRKKQDIEMNNRKVKVHDGNGIFRR---EGWRDLKVGNIVRVE 166
>At5g55660 putative protein
Length = 759
Score = 30.8 bits (68), Expect = 1.7
Identities = 17/51 (33%), Positives = 27/51 (52%)
Query: 414 ESERDRESDGEGVYRRNFVDKREDSEKGVIVKIEGGGDRLFISREDVEGIE 464
ESE ++E D E + ++ E++E G+ K E +L S E+VE E
Sbjct: 509 ESEEEKEDDEEEEKEQEVEEEEEENENGIPDKSEDEAPQLSESEENVESEE 559
>At2g01680 unknown protein
Length = 532
Score = 30.8 bits (68), Expect = 1.7
Identities = 12/33 (36%), Positives = 17/33 (51%)
Query: 152 SSYDWNLIHSATVRTHLDEINSICRVWPEIAAV 184
S D N H A R HL + + R+WPE+ +
Sbjct: 91 SKSDMNAFHVAAKRGHLGIVKELLRLWPELCRI 123
>At4g38480 unknown protein
Length = 471
Score = 30.4 bits (67), Expect = 2.2
Identities = 20/69 (28%), Positives = 33/69 (46%), Gaps = 3/69 (4%)
Query: 401 LEVWSRVKEVVECESERDRESDGEGVYRRNFVDKREDSEKGVI--VKIEGGGDRLFISRE 458
+ VW R ++ S +R S E + RR +DK+ D KG + V GD + +S
Sbjct: 16 VNVWERELGLLPNRSFSNRFSASEDLLRRLGLDKKLDKHKGCVNTVSFNADGD-ILLSGS 74
Query: 459 DVEGIEVWE 467
D + +W+
Sbjct: 75 DDRQVILWD 83
>At3g02260 unknown protein
Length = 5079
Score = 30.0 bits (66), Expect = 2.9
Identities = 20/85 (23%), Positives = 44/85 (51%), Gaps = 16/85 (18%)
Query: 396 GRGGELEVWSRVKEVVECESERDRESDG-------------EGVYRRNFVDKREDSEKGV 442
G GE ++ +R + +++ ++++ + DG + RR + KRE+ +G+
Sbjct: 4587 GVSGENDIGARAENLLDTLADKEGKGDGFLGEKVRALRDATKDEMRRRALRKREELLQGL 4646
Query: 443 IVKIE---GGGDRLFISREDVEGIE 464
++ E GG+R+ +S+ +EG E
Sbjct: 4647 GMRQELSSDGGERIVVSQPILEGFE 4671
>At4g35290 unknown protein
Length = 912
Score = 29.6 bits (65), Expect = 3.8
Identities = 10/29 (34%), Positives = 18/29 (61%)
Query: 346 KSGDLAMADMRRLGDDPWVYLKEKNPSLW 374
+SG + +A + +L D PW +L+ P +W
Sbjct: 563 ESGLVVVAPVTKLNDTPWAFLRPFTPPMW 591
>At4g02220 putative zinc finger protein
Length = 418
Score = 29.6 bits (65), Expect = 3.8
Identities = 24/78 (30%), Positives = 33/78 (41%), Gaps = 6/78 (7%)
Query: 403 VWSRVKEVVECESERDRESDGEGVYRRNFVDKRE--DSEKGVIVKIEGGGDRL----FIS 456
+W + E ESE D E G+ + V KRE D K ++ EG D+ F
Sbjct: 258 LWKEFVLINEDESEYDTEMSGDDEVAKPLVSKREVDDQMKSLMNDFEGDADKKNWVNFQQ 317
Query: 457 REDVEGIEVWESSHYAGA 474
R D +V S +GA
Sbjct: 318 RVDKAPEQVLRYSRSSGA 335
>At5g65330 putative protein
Length = 341
Score = 29.3 bits (64), Expect = 5.0
Identities = 15/51 (29%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Query: 393 VFVGRGGELEVWSRVKEVVECESERDRESDGEGVYR--RNFVDKREDSEKG 441
++ G GEL W + +E V+ + R +E+ R F++K +D +KG
Sbjct: 41 IYYGPDGELRTWPKERETVKDMALRYKEARKRKKSRNLHEFLEKEKDKDKG 91
>At5g56880 unknown protein
Length = 159
Score = 29.3 bits (64), Expect = 5.0
Identities = 18/48 (37%), Positives = 24/48 (49%), Gaps = 2/48 (4%)
Query: 111 PPFSGIDASIVASVRPASEGLSSTFTAA-ENGSVWIAHGGQLSSYDWN 157
P FS D+S++ASV AS F E G W + + S Y+WN
Sbjct: 100 PSFSDSDSSVMASV-DASGLFGCCFEYGNEKGGAWSTNEVRASEYEWN 146
>At5g45850 putative protein
Length = 444
Score = 29.3 bits (64), Expect = 5.0
Identities = 14/48 (29%), Positives = 26/48 (54%)
Query: 172 NSICRVWPEIAAVGTESDAGLHFYNFSGSRHVGSVHWSDPSDPRIFKA 219
NS+ + P ++ + T D G+ N + + SV+ + + PRIFK+
Sbjct: 233 NSVDVLTPVLSRIKTCQDVGVKSENIINPKSIDSVYKTKSTSPRIFKS 280
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.134 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,696,784
Number of Sequences: 26719
Number of extensions: 539242
Number of successful extensions: 1551
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 1518
Number of HSP's gapped (non-prelim): 32
length of query: 478
length of database: 11,318,596
effective HSP length: 103
effective length of query: 375
effective length of database: 8,566,539
effective search space: 3212452125
effective search space used: 3212452125
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149134.10