BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149130.2 + phase: 0
(239 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13940 unknown protein 216 1e-56
At4g13610 DNA (cytosine-5-)-methyltransferase - like protein 33 0.10
At4g07970 hypothetical protein on transposon FARE2.3 31 0.67
At5g65440 unknown protein 30 1.1
At4g32770 sucrose export defective 1 precursor (SXD1) 30 1.1
At3g19510 putative homeobox protein, HAT3.1 30 1.1
At3g13682 hypothetical protein 30 1.1
At1g64330 unknown protein 29 2.5
At2g33590 putative cinnamoyl-CoA reductase 28 3.3
At3g48190 ataxia-telangiectasia mutated protein AtATM 28 4.3
At2g45820 remorin 28 5.7
At1g73760 putative RING zinc finger protein 28 5.7
At1g18410 kinesin-related protein, putative 28 5.7
At5g09320 unknown protein 27 7.4
At3g04750 hypothetical protein 27 7.4
At5g03060 putative protein 27 9.7
At1g80690 unknown protein 27 9.7
>At3g13940 unknown protein
Length = 442
Score = 216 bits (549), Expect = 1e-56
Identities = 108/232 (46%), Positives = 155/232 (66%)
Query: 5 EEPEAKKNLDEKMKNVEVKETALANTEAHVTRHIPPYNSSATTPQEAYVLDKIILAEEWN 64
++ +K LD K+ + V AL T + V R+IPPYN++ATT EAY L+KII +W+
Sbjct: 211 DDAATQKVLDGKLSELGVNTAALEGTSSTVARNIPPYNTAATTANEAYPLEKIIEKGDWS 270
Query: 65 YLQDIYYSLQKEVAADFSLYPSFIRNRINRLKKIEDDSEKKKLSCILSFINYLVKFKDQH 124
+L+DIY+ LQ+E A YP F+RNR+ RL+ I+DD +K+ + + + +LVKFKD++
Sbjct: 271 FLEDIYWLLQQETEAATEAYPVFVRNRLYRLRDIKDDMKKQTVCGAATLLTHLVKFKDRN 330
Query: 125 SMDGISSSKFEKLPYTLYHRFTTMFDVTESRRLPPEKMNLLISYVLVLTLFSDDFRTDYR 184
SM+G S+K K+P +F +MF +ES R+P +K+NLLISYVLVL+L D+F TD
Sbjct: 331 SMNGYDSAKDHKMPDIFRQKFNSMFKDSESDRIPVDKINLLISYVLVLSLHVDNFMTDPE 390
Query: 185 DIVKDLRMSTLTLRPIFEHLGCKFISSQKVSYATLPIPLTFSQIKKRKRKNK 236
DI KDLR+ST+ LR F LGCKF+ + ATLP PL F ++ +R+R K
Sbjct: 391 DIAKDLRISTVELRKHFLQLGCKFLKQNSTTVATLPTPLNFPEVNRRRRARK 442
>At4g13610 DNA (cytosine-5-)-methyltransferase - like protein
Length = 1404
Score = 33.5 bits (75), Expect = 0.10
Identities = 35/141 (24%), Positives = 58/141 (40%), Gaps = 23/141 (16%)
Query: 20 VEVKETALANTEAHVTRHIPPYNSSATTPQEAYVLDKIILAEEWNYLQDIYYSLQKEVAA 79
V++KE NT + + Y + +EAY D +Q++YYS +
Sbjct: 834 VDLKEPKKGNTTSFEVKVRRFYRPDDVSAEEAYASD----------IQEVYYSEDTYI-- 881
Query: 80 DFSLYPSFIRNRINRLKKIEDDSEKKKLSCILSFINYLVKFKDQHSMDGISSSKFEKLPY 139
L P I+ + +KK D + IL + + +F D S+ +KLPY
Sbjct: 882 ---LPPEAIKGKCEVMKKT--DMPLCREYPILDHVYFCDRFYDS------SNGCLKKLPY 930
Query: 140 TLYHRFTTMFDVTESRRLPPE 160
+ +F+T+ D T R E
Sbjct: 931 NMMLKFSTIKDDTLLREKKTE 951
>At4g07970 hypothetical protein on transposon FARE2.3
Length = 201
Score = 30.8 bits (68), Expect = 0.67
Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 7/54 (12%)
Query: 1 MKRDEEPEAKKNLDEKMKNVEVKETALANTEAHVTRHIPPYNSSATTPQEAYVL 54
+K E+PE K DE+M+ E KE N E H + T QEAYV+
Sbjct: 58 VKETEKPENLKQDDEEMEGEEGKEDEEENVEEH-------DEHNETEDQEAYVI 104
>At5g65440 unknown protein
Length = 1050
Score = 30.0 bits (66), Expect = 1.1
Identities = 15/58 (25%), Positives = 29/58 (49%), Gaps = 5/58 (8%)
Query: 36 RHIPPYNSSATTPQEAYVLDKIILAEEWNYLQDIYYSLQKEVAADFSLY-----PSFI 88
RH+ N E +D ++LA + ++ + SL+ E++ D +++ PSFI
Sbjct: 602 RHLLDTNDLLNNRSEGVPVDPMVLAASYQKMKSLILSLKNEISTDIAIHDCNVLPSFI 659
>At4g32770 sucrose export defective 1 precursor (SXD1)
Length = 488
Score = 30.0 bits (66), Expect = 1.1
Identities = 19/54 (35%), Positives = 27/54 (49%), Gaps = 7/54 (12%)
Query: 154 SRRLPPEKMNLLISYVLVLTLF------SDDFRTDYRDIVKDLRMSTLTLRPIF 201
++ +PPE+ N +S T F DD RTDY + VK R T RP++
Sbjct: 183 NKEVPPEEFNRRVSEGFQATPFWHQGHICDDGRTDYAETVKSARWEYST-RPVY 235
>At3g19510 putative homeobox protein, HAT3.1
Length = 723
Score = 30.0 bits (66), Expect = 1.1
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 4/53 (7%)
Query: 89 RNRINRLKKIEDDSE----KKKLSCILSFINYLVKFKDQHSMDGISSSKFEKL 137
+N+ ++ +D E KKKL L+ INY D +S++G S EK+
Sbjct: 162 KNKTMNKGQVREDDEYTRIKKKLRYFLNRINYEQSLIDAYSLEGWKGSSLEKI 214
>At3g13682 hypothetical protein
Length = 746
Score = 30.0 bits (66), Expect = 1.1
Identities = 10/30 (33%), Positives = 22/30 (73%)
Query: 83 LYPSFIRNRINRLKKIEDDSEKKKLSCILS 112
LY R + N++K+++++S + KLSC+++
Sbjct: 667 LYTILSREQANKIKELDENSNESKLSCLMN 696
>At1g64330 unknown protein
Length = 555
Score = 28.9 bits (63), Expect = 2.5
Identities = 28/112 (25%), Positives = 49/112 (43%), Gaps = 5/112 (4%)
Query: 11 KNLDEKMKNVEVKETALANTEAHVTRHIPPYNSSATTPQEA-YVLDKIILAEEWNYLQDI 69
++L EKM N+EVK L+N + VT + EA ++ ++ +L E+ +
Sbjct: 359 ESLMEKMSNIEVK-LRLSNQKLRVTEQVLTEKEGELKRIEAKHLEEQALLEEKIATTHET 417
Query: 70 YYSLQKEVAADFSLYPSFIRNRINRLKKIEDDSEKKKLSCILSFINYLVKFK 121
Y L KE++ S I NR L + ++ K ++ L+ K
Sbjct: 418 YRGLIKEIS---ERVDSTILNRFQSLSEKLEEKHKSYEKTVVEATKMLLTAK 466
>At2g33590 putative cinnamoyl-CoA reductase
Length = 321
Score = 28.5 bits (62), Expect = 3.3
Identities = 17/59 (28%), Positives = 27/59 (44%)
Query: 98 IEDDSEKKKLSCILSFINYLVKFKDQHSMDGISSSKFEKLPYTLYHRFTTMFDVTESRR 156
++++ +KL NY K+ D +SS K +KL +T T+ D ES R
Sbjct: 257 VKEEIVVEKLKSFYPHYNYPKKYIDAEDRVKVSSEKLQKLGWTYRPLEETLVDSVESYR 315
>At3g48190 ataxia-telangiectasia mutated protein AtATM
Length = 3856
Score = 28.1 bits (61), Expect = 4.3
Identities = 17/35 (48%), Positives = 20/35 (56%), Gaps = 2/35 (5%)
Query: 205 GCKFISSQKVSYA--TLPIPLTFSQIKKRKRKNKN 237
G +F+SSQ SY T T+S KKR RK KN
Sbjct: 599 GTQFVSSQPTSYKHFTSADLFTYSGKKKRGRKRKN 633
>At2g45820 remorin
Length = 190
Score = 27.7 bits (60), Expect = 5.7
Identities = 29/113 (25%), Positives = 50/113 (43%), Gaps = 10/113 (8%)
Query: 3 RDEEPEAKKNLDEKMKNVE-VKETALANTEAHVTRHIPPYNSSATTPQEAYVLD------ 55
++ P + DEK+ N V+ ALA E + H P SS + ++ + D
Sbjct: 22 KEPTPAPVEVADEKIHNPPPVESKALAVVEKPIEEHTPKKASSGSADRDVILADLEKEKK 81
Query: 56 -KIILAEEWNYLQDIYYSLQKEVAADFSLYPSFIRNRIN-RLKKIEDDSEKKK 106
I A E + QK++ +D + + + + +L+KIE+ EKKK
Sbjct: 82 TSFIKAWEESEKSKAENRAQKKI-SDVHAWENSKKAAVEAQLRKIEEKLEKKK 133
>At1g73760 putative RING zinc finger protein
Length = 367
Score = 27.7 bits (60), Expect = 5.7
Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 11/46 (23%)
Query: 201 FEHLGCKFISSQKVSYATLPIPLTFS--------QIKKRKRKNKNK 238
F LGC +SQ+VS +P + S +IKK K+KNKNK
Sbjct: 72 FRGLGCTTSASQQVS---VPAVIRSSADWDASNFKIKKTKKKNKNK 114
>At1g18410 kinesin-related protein, putative
Length = 1081
Score = 27.7 bits (60), Expect = 5.7
Identities = 24/124 (19%), Positives = 52/124 (41%), Gaps = 6/124 (4%)
Query: 4 DEEPEAKKNLDEKMKNVEVKETALANTEAHVTRHIPPYNSSATTPQEAYVLDKIILAEEW 63
+E K L+EK K+ E + H I T ++AY + + +
Sbjct: 328 EENETEKSKLEEKKKDKEEDMVGIEKENGHYNLEISTLRRELETTKKAYEQQCLQMESKT 387
Query: 64 N----YLQDIYYSLQKEVAADFSLYPSFIRNRINRLKKIEDDSEKKKLSCILSFINYLVK 119
++D L+ ++ D S+ + R+ L+K+ +++ K++ + + L K
Sbjct: 388 KGATAGIEDRVKELE-QMRKDASVARKALEERVRELEKMGKEADAVKMN-LEEKVKELQK 445
Query: 120 FKDQ 123
+KD+
Sbjct: 446 YKDE 449
>At5g09320 unknown protein
Length = 712
Score = 27.3 bits (59), Expect = 7.4
Identities = 15/59 (25%), Positives = 29/59 (48%)
Query: 3 RDEEPEAKKNLDEKMKNVEVKETALANTEAHVTRHIPPYNSSATTPQEAYVLDKIILAE 61
RD K +M ++E + A A+ PP S++TT + +LD++I+++
Sbjct: 490 RDRSKNRKPLQRGRMLSIEAIQAVQALKRANPLLPPPPVPSTSTTSSSSALLDRVIISK 548
>At3g04750 hypothetical protein
Length = 661
Score = 27.3 bits (59), Expect = 7.4
Identities = 25/86 (29%), Positives = 37/86 (42%), Gaps = 18/86 (20%)
Query: 81 FSLYPSFIRNRINR--------LKKIEDDSEKKKLSC------ILSFINYL----VKFKD 122
F LY S IR+R++ +K SE K++ C LS NYL VKF
Sbjct: 118 FGLYSSMIRHRVSPDRQTFLYLMKASSFLSEVKQIHCHIIVSGCLSLGNYLWNSLVKFYM 177
Query: 123 QHSMDGISSSKFEKLPYTLYHRFTTM 148
+ G++ F ++P+ F M
Sbjct: 178 ELGNFGVAEKVFARMPHPDVSSFNVM 203
>At5g03060 putative protein
Length = 292
Score = 26.9 bits (58), Expect = 9.7
Identities = 28/99 (28%), Positives = 49/99 (49%), Gaps = 10/99 (10%)
Query: 42 NSSATTPQEAYVLDKIILAEEWNYLQDIYYSLQKEVAADFSLYPSFIR-NRINRLKKIED 100
+S AT + V+ + ILA + L++ Y +L+K+ S+ SF + N +N + K +
Sbjct: 18 SSVATPTRRNLVVQEEILATKHEILKENYENLEKDYK---SIEESFKQMNEMNEIMKFQY 74
Query: 101 DSEKKKL-SCILSFINYLVKFKDQ-----HSMDGISSSK 133
+ K+L ILS + L K + + M G+ S K
Sbjct: 75 QKQIKELEEKILSLLKDLEKERSEKEEYMKEMKGMISEK 113
>At1g80690 unknown protein
Length = 227
Score = 26.9 bits (58), Expect = 9.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Query: 87 FIRNRINRLKKIEDDSEKKKLSCILS 112
F NR+N+ K E ++EKKKL+ + S
Sbjct: 157 FGNNRVNQDKSCEAENEKKKLTSVSS 182
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.134 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,307,240
Number of Sequences: 26719
Number of extensions: 215521
Number of successful extensions: 668
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 659
Number of HSP's gapped (non-prelim): 17
length of query: 239
length of database: 11,318,596
effective HSP length: 96
effective length of query: 143
effective length of database: 8,753,572
effective search space: 1251760796
effective search space used: 1251760796
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC149130.2