BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149040.5 - phase: 0
(476 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53020 putative protein 32 0.77
At5g52280 hyaluronan mediated motility receptor-like protein 32 0.77
At3g51730 unknown protein 32 0.77
At4g36990 heat shock transcription factor HSF4 32 1.0
At1g79830 unknown protein (At1g79830) 32 1.0
At5g19900 PRLI-interacting factor A 31 1.3
At1g16690 Unknown protein 31 1.3
At1g73860 kinesin-related protein 31 1.7
At5g49680 putative protein 30 3.8
At5g03060 putative protein 30 3.8
At4g31340 unknown protein 30 3.8
At1g37160 hypothetical protein 30 3.8
At5g61490 putative protein 29 5.0
At3g29375 unknown protein 29 5.0
At1g17930 unknown protein 29 6.5
At5g23890 unknown protein 28 8.5
At2g25010 unknown protein 28 8.5
At2g18540 putative vicilin storage protein (globulin-like) 28 8.5
>At5g53020 putative protein
Length = 720
Score = 32.0 bits (71), Expect = 0.77
Identities = 22/71 (30%), Positives = 36/71 (49%), Gaps = 3/71 (4%)
Query: 384 QEHLAMESREKQVWKARYNQAENLIMTLDGRNEQ---KTHENLMLKKELAKARRELEEKD 440
QEH + R+K+ + + L L N+Q K HE L E+ K R++LE+KD
Sbjct: 155 QEHSSELWRQKKTFLELASSQRQLEAELSRANKQIEAKGHELEDLSLEINKMRKDLEQKD 214
Query: 441 ELLMRDSKRAR 451
+L K+++
Sbjct: 215 RILAVMMKKSK 225
>At5g52280 hyaluronan mediated motility receptor-like protein
Length = 853
Score = 32.0 bits (71), Expect = 0.77
Identities = 32/167 (19%), Positives = 67/167 (39%), Gaps = 8/167 (4%)
Query: 307 TNAPTGQRKAFVDAWSKVRRKSMKHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSS 366
++A + + + W K R + + L + VA A + + ++ +R + +
Sbjct: 671 SSAAATETEKIIQEWRKERDEFERKLSLAKEVAKTAQKELTLTKSSNDDKE-TRLRNLKT 729
Query: 367 STPSMPLPLLPATQDMYQEHLAMESREKQVWKARYN---QAENLIMTLDGRNEQKTHENL 423
+ L QE + + KQV + + + E + LD R E ++ EN
Sbjct: 730 EVEGLSLQYSELQNSFVQEKMENDELRKQVSNLKVDIRRKEEEMTKILDARMEARSQENG 789
Query: 424 MLKKELAKARREL---EEKDELLMRDSKRARGR-RDFFARYCDSDSE 466
++ L+K EL + K+ + R+ K R + R+ + + E
Sbjct: 790 HKEENLSKLSDELAYCKNKNSSMERELKEMEERYSEISLRFAEVEGE 836
>At3g51730 unknown protein
Length = 213
Score = 32.0 bits (71), Expect = 0.77
Identities = 25/76 (32%), Positives = 32/76 (41%), Gaps = 17/76 (22%)
Query: 153 LLIYGLILFPNLDDFVDMNAIEIFHSKNPVPTLL-----------------ADTYHAIHD 195
LL+ GLIL + FVD E +K V TL A+ +HD
Sbjct: 10 LLLLGLILVSDARSFVDSTISEKVSNKEDVCTLCEEYVTDALSYLEKNVTQAEIIEDLHD 69
Query: 196 RTLKGRGYILCCISLL 211
R + RGY CISL+
Sbjct: 70 RCSQLRGYSQQCISLV 85
>At4g36990 heat shock transcription factor HSF4
Length = 284
Score = 31.6 bits (70), Expect = 1.0
Identities = 14/34 (41%), Positives = 21/34 (61%)
Query: 405 ENLIMTLDGRNEQKTHENLMLKKELAKARRELEE 438
EN++ L G NE+ EN L ELA A+++ +E
Sbjct: 150 ENMVADLSGENEKLKRENNNLSSELAAAKKQRDE 183
>At1g79830 unknown protein (At1g79830)
Length = 918
Score = 31.6 bits (70), Expect = 1.0
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 2/90 (2%)
Query: 367 STPSMPLPL--LPATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLM 424
S PS P+ L L A Q + L E + + Y A+ TL+GR Q E
Sbjct: 670 SLPSSPIQLSCLRAEQGQLSKSLEKERQRAAENRQEYLAAKEEADTLEGRANQLEVEIRE 729
Query: 425 LKKELAKARRELEEKDELLMRDSKRARGRR 454
L+++ + +E+ +EL+ +D +R + R
Sbjct: 730 LRRKHKQELQEVLLHNELIQKDLEREKASR 759
>At5g19900 PRLI-interacting factor A
Length = 494
Score = 31.2 bits (69), Expect = 1.3
Identities = 22/71 (30%), Positives = 30/71 (41%)
Query: 402 NQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKRARGRRDFFARYC 461
N EN + D Q ENL LK+ L REL + L +R +D
Sbjct: 306 NVLENRVDDQDSHIAQLEEENLTLKERLFLMERELGDMRRRLQYLERRDMVAQDANEEVV 365
Query: 462 DSDSESDNPPT 472
+++SESD T
Sbjct: 366 ENESESDGDDT 376
>At1g16690 Unknown protein
Length = 439
Score = 31.2 bits (69), Expect = 1.3
Identities = 44/165 (26%), Positives = 76/165 (45%), Gaps = 25/165 (15%)
Query: 327 KSMKHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSSSTP---SMPLPLLPATQDMY 383
+++K+L ++ GV H Y+ W R EI P +R + P + P + + +
Sbjct: 173 EALKYLSIKYGVFHAIYSYWKNKR--EIWQK-PILRRLQPPPPVNDTNPYNVFRPREKAH 229
Query: 384 QEHL-AMESRE---------KQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKAR 433
+ H M+ RE +QV + +QA+ ++ L R E+K + E++ R
Sbjct: 230 RLHARRMQQRENNAQSFEKLRQV-RRNLDQAKTILEALIKREEKKRD---FMASEVSLQR 285
Query: 434 RELEEKDEL-LMRDSKRARGRRDFFA-RYCDSDSE---SDNPPTT 473
+L+ K+E L+ DS G A R+ S+ E SD+P TT
Sbjct: 286 IQLKYKNETELLEDSLALAGFPLSTAYRFGSSEDEFMDSDDPTTT 330
>At1g73860 kinesin-related protein
Length = 1050
Score = 30.8 bits (68), Expect = 1.7
Identities = 15/59 (25%), Positives = 32/59 (53%)
Query: 393 EKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKRAR 451
EK +W+ + E ++ L +N+Q E LK+EL +R+ E++ + +K+++
Sbjct: 238 EKSMWEEKKKHEEEDMVKLMKQNDQHNLEISALKQELETTKRKYEQQYSQIESQTKKSK 296
>At5g49680 putative protein
Length = 1378
Score = 29.6 bits (65), Expect = 3.8
Identities = 29/117 (24%), Positives = 51/117 (42%), Gaps = 16/117 (13%)
Query: 368 TPSMPL-PLLPATQDMYQEHLAMESREKQVWK--------ARYNQAENLIMTLDGRNEQK 418
TP + + PL T + +M SR+ QV AR +A N + L G + +
Sbjct: 725 TPDLKVKPLKELTFNSRNITASMTSRQFQVMTDVLSNLLFARLPKAHNDSLKLSGEEDDE 784
Query: 419 THENL-------MLKKELAKARRELEEKDELLMRDSKRARGRRDFFARYCDSDSESD 468
E + + + ELAK E +E+D +++ D R + + + + + ESD
Sbjct: 785 VEEEIDEVVPDGIEEVELAKIELEAKERDRMMLLDDIRKLTQNESNSGNINLEKESD 841
>At5g03060 putative protein
Length = 292
Score = 29.6 bits (65), Expect = 3.8
Identities = 15/65 (23%), Positives = 33/65 (50%)
Query: 395 QVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKRARGRR 454
++ K Y E +++ +Q N ++K + K +ELEEK L++D ++ R +
Sbjct: 40 EILKENYENLEKDYKSIEESFKQMNEMNEIMKFQYQKQIKELEEKILSLLKDLEKERSEK 99
Query: 455 DFFAR 459
+ + +
Sbjct: 100 EEYMK 104
>At4g31340 unknown protein
Length = 437
Score = 29.6 bits (65), Expect = 3.8
Identities = 16/70 (22%), Positives = 35/70 (49%)
Query: 381 DMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKD 440
++ + L +++EK +AR N+AE + L+ ++ N K ++ K R ++ +
Sbjct: 122 EVLKNFLEQKNKEKDSTEARTNEAEKKLRELNSSLDKLQKTNEEQKNKIGKLERAIKIAE 181
Query: 441 ELLMRDSKRA 450
E ++R A
Sbjct: 182 EEMLRTKLEA 191
>At1g37160 hypothetical protein
Length = 480
Score = 29.6 bits (65), Expect = 3.8
Identities = 14/46 (30%), Positives = 25/46 (53%)
Query: 401 YNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRD 446
YN E + EQ+ +E+ K E+A+ +R + E+ EL+ +D
Sbjct: 14 YNMDETESSSSRPEREQREYESFRRKAEIARGKRAMTERYELIDKD 59
>At5g61490 putative protein
Length = 260
Score = 29.3 bits (64), Expect = 5.0
Identities = 14/42 (33%), Positives = 23/42 (54%)
Query: 205 LCCISLLYRWFISHLPSSFHDNSENWSYSQRIMALTPNEVVW 246
LC SLL + + L +FH + S + +M+LT ++ VW
Sbjct: 118 LCNTSLLQPFMLDRLYDAFHVFQTDPSVQRMVMSLTSDKAVW 159
>At3g29375 unknown protein
Length = 176
Score = 29.3 bits (64), Expect = 5.0
Identities = 17/43 (39%), Positives = 23/43 (52%), Gaps = 7/43 (16%)
Query: 408 IMTLDGRNEQKTH-------ENLMLKKELAKARRELEEKDELL 443
I+ ++G NE + H EN LKK L + ELE+KD L
Sbjct: 13 IILVEGNNESQEHKWRSMKQENEELKKRLRVMKEELEDKDSEL 55
>At1g17930 unknown protein
Length = 478
Score = 28.9 bits (63), Expect = 6.5
Identities = 58/288 (20%), Positives = 106/288 (36%), Gaps = 59/288 (20%)
Query: 45 VEEKLVHTLVQFYDPSFRCFTFPDFQLVPTLEAYSNLVGLPIAEKTPFTGPGTSLTPLVI 104
+ L+ LV+ + F FP ++ TL+ S ++GL + K P+V
Sbjct: 62 LNNSLISALVERWRRETNTFHFPCGEMTITLDEVSLILGLAVDGK-----------PVVG 110
Query: 105 AKDLYLKTSDVSNHL---ITKSHIRG--FTSKYLLD---QANLGTTRQDTLEAILALLIY 156
K+ S V L + K + G T+K+L + + G T ++ A LIY
Sbjct: 111 VKEKDEDPSQVCLRLLGKLPKGELSGNRVTAKWLKESFAECPKGATMKEIEYHTRAYLIY 170
Query: 157 --GLILFPNLD-DFVDMNAIEIFHSKNPV------PTLLADTYHAIHDRTLKGRGYILCC 207
G +F D + ++ + +F LA Y I + + + + I C
Sbjct: 171 IVGSTIFATTDPSKISVDYLILFEDFEKAGEYAWGAAALAFLYRQIGNASQRSQSIIGGC 230
Query: 208 ISLLYRWFISHL--------------------PSSFHDNSENWSYSQRIMALTPNEVVW- 246
++LL W HL ++ + Y + + L P+ V W
Sbjct: 231 LTLLQCWSYFHLNIDRPKRTTRQFPLALLWKGRQQSRSKNDLFKYRKALDDLDPSNVSWC 290
Query: 247 -------LTPAAQVKEIIMGCGDFLNVPLLGTRGGINYNPELAMRQFG 287
+ P + +++G L+G + + P+ M+QFG
Sbjct: 291 PFEGDLDIVPQSFKDNLLLGRS---RTKLIGPKVVEWHFPDRCMKQFG 335
>At5g23890 unknown protein
Length = 946
Score = 28.5 bits (62), Expect = 8.5
Identities = 26/72 (36%), Positives = 38/72 (52%), Gaps = 13/72 (18%)
Query: 383 YQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKE----------LAKA 432
+++ L+ME REK +A AE + L+ E++ ENL L KE L++
Sbjct: 671 FEKELSME-REKI--EAVEKMAELAKVELEQLREKREEENLALVKERAAVESEMEVLSRL 727
Query: 433 RRELEEKDELLM 444
RR+ EEK E LM
Sbjct: 728 RRDAEEKLEDLM 739
>At2g25010 unknown protein
Length = 509
Score = 28.5 bits (62), Expect = 8.5
Identities = 42/196 (21%), Positives = 76/196 (38%), Gaps = 15/196 (7%)
Query: 45 VEEKLVHTLVQFYDPSFRCFTFPDFQLVPTLEAYSNLVGLPIAEKTPFTGPGTS-LTPLV 103
+ L+ LV+ + F P ++ TL+ + ++GL I + P G +
Sbjct: 71 LNNSLISALVERWRRETNTFHLPLGEMTITLDEVALVLGLEI-DGDPIVGSKVGDEVAMD 129
Query: 104 IAKDLYLKTSDVSNHLITKSHIR----GFTSKYLLDQANLGTTRQDTLEAILALLIYGLI 159
+ L K +N + S ++ T + A+ + T +L L+ G
Sbjct: 130 MCGRLLGKLPSAANKEVNCSRVKLNWLKRTFSECPEDASFDVVKCHTRAYLLYLI--GST 187
Query: 160 LFPNLD-DFVDMNAIEIFHSKNPV------PTLLADTYHAIHDRTLKGRGYILCCISLLY 212
+F D D V + + +F + LA Y A+ + +LK + I C++LL
Sbjct: 188 IFATTDGDKVSVKYLPLFEDFDQAGRYAWGAAALACLYRALGNASLKSQSNICGCLTLLQ 247
Query: 213 RWFISHLPSSFHDNSE 228
W HL + SE
Sbjct: 248 CWSYFHLDIGRPEKSE 263
>At2g18540 putative vicilin storage protein (globulin-like)
Length = 699
Score = 28.5 bits (62), Expect = 8.5
Identities = 17/68 (25%), Positives = 32/68 (47%), Gaps = 6/68 (8%)
Query: 384 QEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELL 443
+E + + RE+Q K R +A E+K E + ++E + R+E EE + +
Sbjct: 534 REEVERKRREEQERKRREEEARKR------EEERKREEEMAKRREQERQRKEREEVERKI 587
Query: 444 MRDSKRAR 451
+ +R R
Sbjct: 588 REEQERKR 595
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.136 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,151,975
Number of Sequences: 26719
Number of extensions: 499778
Number of successful extensions: 1370
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1354
Number of HSP's gapped (non-prelim): 25
length of query: 476
length of database: 11,318,596
effective HSP length: 103
effective length of query: 373
effective length of database: 8,566,539
effective search space: 3195319047
effective search space used: 3195319047
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149040.5