BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148995.13 - phase: 0
(160 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03230 unknown protein (At5g03230) 132 1e-31
At5g60680 unknown protein 127 3e-30
At3g45210 unknown protein 119 7e-28
At2g28400 unknown protein 103 3e-23
At4g04630 unknown protein 89 8e-19
At1g61930 unknown protein 84 4e-17
At1g11700 unknown protein 84 4e-17
At4g21970 hypothetical protein 80 5e-16
At4g21930 unknown protein 75 2e-14
At4g26950 putative protein 72 1e-13
At3g15040 unknown protein 69 1e-12
At1g29640 unknown protein 61 2e-10
At2g34340 unknown protein 59 1e-09
At2g16640 putative chloroplast outer membrane protein 35 0.017
At5g45630 putative protein 32 0.11
At3g16620 putative GTP-binding protein 32 0.15
At5g10070 unknown protein 30 0.56
At5g54460 unknown protein 30 0.73
At5g04240 zinc finger protein - like 29 0.95
At3g27530 unknown protein 29 0.95
>At5g03230 unknown protein (At5g03230)
Length = 166
Score = 132 bits (331), Expect = 1e-31
Identities = 74/167 (44%), Positives = 99/167 (58%), Gaps = 11/167 (6%)
Query: 3 SSRKSFLSRTSYIFPETNFNQKSSQGKELEFDEADVWNMSYSNSNTNIEPKKGVP--GLK 60
+SRK F + YI+PE + S EFDE+D+ N+ + + K+ + L+
Sbjct: 2 ASRKLFFVKPKYIYPEPK--PEMSDENVFEFDESDIHNLGDHQLPNSFDAKRSISISRLR 59
Query: 61 RVSRKMEANNKVNPLASSSLPMNIPDWSKILKEEYKKKK---ESSDDEDEGDYD----GV 113
R K + + SLP+NIPDWSKILK EY+ + SDD+DE D D G
Sbjct: 60 RKPTKTGDSGNREITKTGSLPVNIPDWSKILKSEYRGHAIPDDDSDDDDEDDDDSNDGGR 119
Query: 114 VQLPPHEYLARTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED 160
+PPHEYLAR RG+S +VHEG G T KGRDLR +RNAIW+K+GF+D
Sbjct: 120 RMIPPHEYLARRRGSSFTVHEGIGGTAKGRDLRRLRNAIWEKIGFQD 166
>At5g60680 unknown protein
Length = 163
Score = 127 bits (318), Expect = 3e-30
Identities = 75/161 (46%), Positives = 104/161 (64%), Gaps = 8/161 (4%)
Query: 6 KSFLSRTSYIFPETNF-NQKSSQGKELEFDEADVWNMSYSNSNTNIEP-KKGVPGLKRVS 63
KS+ +R SY F T+ + ++ LEFDE+D++N +S+S V K+ S
Sbjct: 5 KSYYARPSYRFLGTDQPSYFTASDSGLEFDESDLFNPIHSDSPDFCRKISSSVRSGKKSS 64
Query: 64 RKMEANNKVNPLASSSLPMNIPDWSKILKEEYKKKK----ESSDDEDEGDYDGVVQLPPH 119
+ A + A+SSLP+N+PDWSKIL+ EY+ + E +DD+D+ + DG LPPH
Sbjct: 65 NRPSAASSA--AAASSLPVNVPDWSKILRGEYRDNRRRSIEDNDDDDDDNEDGGDWLPPH 122
Query: 120 EYLARTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED 160
E+LA+TR AS SVHEG GRTLKGRDL VRNAI++K GF+D
Sbjct: 123 EFLAKTRMASFSVHEGVGRTLKGRDLSRVRNAIFEKFGFQD 163
>At3g45210 unknown protein
Length = 148
Score = 119 bits (298), Expect = 7e-28
Identities = 72/158 (45%), Positives = 95/158 (59%), Gaps = 13/158 (8%)
Query: 3 SSRKSFLSRTSYIFPETNFNQKSSQGKELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRV 62
++RKS+ R S+ F T+ + E EFDE+D+ YSN + + E ++ L
Sbjct: 4 ATRKSYYQRPSHRFLPTDRTYNVTGDSEFEFDESDL----YSNRSDSPEFRRK---LITS 56
Query: 63 SRKMEANNKVNPLASSSLPMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYL 122
+RK +SSLPMN+ +WSKIL +E +K E+ DD EG +LPPHEYL
Sbjct: 57 NRKSSPATVTTTTVASSLPMNVQNWSKILGKENRKSIENDDDGGEG------KLPPHEYL 110
Query: 123 ARTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED 160
A+TR AS SVHEG GRTLKGRD+ VRNAI +K GF D
Sbjct: 111 AKTRMASFSVHEGIGRTLKGRDMSRVRNAILEKTGFLD 148
>At2g28400 unknown protein
Length = 162
Score = 103 bits (258), Expect = 3e-23
Identities = 69/166 (41%), Positives = 101/166 (60%), Gaps = 10/166 (6%)
Query: 1 MASSRKSFLSRTSYIFPETNFNQKSSQGKELEFDEADVWNM-SYSNSNTNIEPKKGVPGL 59
MA+S K + R S+ F T+ Q + + E DE D++N S S+S+ + G
Sbjct: 1 MATS-KCYYPRPSHRFFTTD--QHVTATSDFELDEWDLFNTGSDSSSSFSFSDLTITSGR 57
Query: 60 KRVSRKMEANNKVNPLASSSLPMNIPDWSKILKEEYKKKKESSDDED-EGDY----DGVV 114
+R++ + A+SSLP+N+PDWSKIL +E +++++ S++E+ +GD +G
Sbjct: 58 TGTNRQIHGGSDSGK-AASSLPVNVPDWSKILGDESRRQRKISNEEEVDGDEILCGEGTR 116
Query: 115 QLPPHEYLARTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED 160
++PPHE LA R AS SVHEG GRTLKGRDL VRN I+K G ED
Sbjct: 117 RVPPHELLANRRMASFSVHEGAGRTLKGRDLSRVRNTIFKIRGIED 162
>At4g04630 unknown protein
Length = 168
Score = 89.4 bits (220), Expect = 8e-19
Identities = 57/143 (39%), Positives = 80/143 (55%), Gaps = 18/143 (12%)
Query: 32 EFDEADVWNMSYSNSNTNIE---PKKGVPGLKRVSRK---MEANNKVNPLASSSLPMNIP 85
EF E DVW++ ++ E PK S + + +V+ + SS PMN+P
Sbjct: 26 EFQEEDVWSVLREGETSSPEMKIPKSHFSSSSSSSSSPWNIRRSKEVSGVKQSSAPMNVP 85
Query: 86 DWSKILKEEYKKKKES------SDDEDEGDYDGVVQLPPHEY----LARTRGASLSVHEG 135
DWSK+ + ++ S +DD+DE D DG + +PPHE+ LART+ +S S+ EG
Sbjct: 86 DWSKVYGDSKSNRRSSHLHSHAADDDDEDD-DGCM-VPPHEWVARKLARTQISSFSMCEG 143
Query: 136 KGRTLKGRDLRSVRNAIWKKVGF 158
GRTLKGRDL VRNA+ K GF
Sbjct: 144 VGRTLKGRDLSKVRNAVLSKTGF 166
>At1g61930 unknown protein
Length = 203
Score = 83.6 bits (205), Expect = 4e-17
Identities = 42/90 (46%), Positives = 59/90 (64%), Gaps = 9/90 (10%)
Query: 77 SSSLPMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYLARTR--------GA 128
+SS P+N+PDWSKI + + + DDED+ D + + +PPHEYLA+++ G
Sbjct: 113 ASSAPVNVPDWSKIYRVDSVESIHELDDEDDEDEESGM-MPPHEYLAKSQARRSRKIGGG 171
Query: 129 SLSVHEGKGRTLKGRDLRSVRNAIWKKVGF 158
SV +G GRTLKGR+LR VR+AIW + GF
Sbjct: 172 GASVFDGVGRTLKGRELRRVRDAIWSQTGF 201
>At1g11700 unknown protein
Length = 201
Score = 83.6 bits (205), Expect = 4e-17
Identities = 44/95 (46%), Positives = 60/95 (62%), Gaps = 9/95 (9%)
Query: 71 KVNPLASSSLPMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYLARTR---- 126
+V +SS P+N+PDWSKI + + SD+E+E D + +PPHEYLA+++
Sbjct: 107 RVERQLASSAPVNVPDWSKIYRVNSVESIHESDEEEEEDSG--MMMPPHEYLAKSQQRRS 164
Query: 127 ---GASLSVHEGKGRTLKGRDLRSVRNAIWKKVGF 158
G SV EG GRTLKGR+LR VR+AIW + GF
Sbjct: 165 RKSGGGGSVFEGVGRTLKGRELRRVRDAIWSQTGF 199
>At4g21970 hypothetical protein
Length = 181
Score = 80.1 bits (196), Expect = 5e-16
Identities = 58/145 (40%), Positives = 74/145 (51%), Gaps = 25/145 (17%)
Query: 32 EFDEADVWNMSYSNSNTNIEPKKGVPG------LKRVSRKMEANNKVNPLAS-SSLPMNI 84
EF E +VW++ + E K +R + N+V+ A SS PMNI
Sbjct: 6 EFQEEEVWSVLRESETQGPEMKMSKSNNLFSASSSSSARYIPKGNEVSGGAKQSSAPMNI 65
Query: 85 PDWSKILKEEYKKKKESS-------DDEDEGDYDGVVQLPPHEY----LARTRGASLSVH 133
PDWSK+ Y KK SS DD+DEG +PPHE LART+ +S S+
Sbjct: 66 PDWSKVYG--YSKKNTSSHLHSWAIDDDDEGS-----MVPPHELVAKRLARTQISSFSMC 118
Query: 134 EGKGRTLKGRDLRSVRNAIWKKVGF 158
EG GRTLKGRDL RNA+ + GF
Sbjct: 119 EGIGRTLKGRDLSKTRNAVLTRTGF 143
>At4g21930 unknown protein
Length = 183
Score = 74.7 bits (182), Expect = 2e-14
Identities = 54/156 (34%), Positives = 76/156 (48%), Gaps = 35/156 (22%)
Query: 30 ELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRVSRKMEANN--------KVNPLA----- 76
ELE E DVW++ +EP + +R +EA+ +V+ L
Sbjct: 34 ELELMEEDVWSV--------VEPDEPKELGAWNARSLEASGSEWRRKGGRVSDLTVPSDG 85
Query: 77 ------SSSLPMNIPDWSKILK-EEYKKKKESSDDEDEGDYDGV----VQLPPHEYLA-- 123
++S P+ +PDWSKILK E K +++D D D +PPHEY+A
Sbjct: 86 QRKRHVATSAPVKVPDWSKILKVESVKSMHNNNNDNDNADVADCDWESAMVPPHEYVAAR 145
Query: 124 -RTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGF 158
R SV G GRTLKGRD+R VR+A+W + GF
Sbjct: 146 SRNGDGGSSVFLGVGRTLKGRDMRRVRDAVWSQTGF 181
>At4g26950 putative protein
Length = 144
Score = 72.0 bits (175), Expect = 1e-13
Identities = 52/140 (37%), Positives = 73/140 (52%), Gaps = 25/140 (17%)
Query: 32 EFDEADVWN---------MSYSNSNT--NIEPKKGVPGLKRVSRKMEANNKVNPLASSSL 80
+F E DVW+ M SN T + + +P R+ + + + P+ S+
Sbjct: 15 DFQEDDVWDVLDGYQSPFMISSNHTTKPSFSTQTLLPSEPRMIPERQRTEGMAPMRQQSV 74
Query: 81 PMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPPHEY-LARTRGASLS-VHEGKGR 138
P+N+PDWS + + KK K+ DDE+ + P EY L R+R +S S V EG GR
Sbjct: 75 PVNVPDWSMVQR---KKTKKVVDDEN---------VSPEEYFLRRSRSSSSSSVMEGVGR 122
Query: 139 TLKGRDLRSVRNAIWKKVGF 158
LKGRDL VRNAI K+ GF
Sbjct: 123 KLKGRDLSKVRNAILKQTGF 142
>At3g15040 unknown protein
Length = 243
Score = 68.9 bits (167), Expect = 1e-12
Identities = 40/88 (45%), Positives = 57/88 (64%), Gaps = 7/88 (7%)
Query: 79 SLPMNIPDWSKILKEEYKKKKESSD--DEDEGDYDGVVQLPPHEYLARTRGAS----LSV 132
S P+ +P S + +KK+ + +D D+DE + +G + LPPHE +AR+ S SV
Sbjct: 157 SAPVQVPLVSSAMMNRHKKEFKLTDVVDDDEEEEEGEM-LPPHEIVARSLAQSSLLSCSV 215
Query: 133 HEGKGRTLKGRDLRSVRNAIWKKVGFED 160
EG GRTLKGRDLR VRNA++++ GF D
Sbjct: 216 LEGAGRTLKGRDLRQVRNAVFRRTGFID 243
>At1g29640 unknown protein
Length = 140
Score = 61.2 bits (147), Expect = 2e-10
Identities = 50/145 (34%), Positives = 67/145 (45%), Gaps = 29/145 (20%)
Query: 32 EFDEADV-WNMSY--------SNSNTNIEPKKGVPGLKRVSRKMEANNKVNPLAS----- 77
EFDE+++ ++ +Y N N P +RV K +K PL S
Sbjct: 4 EFDESEIIFSDNYFPIRRREDGNEKENNRPVDFRENSERVWNKSSRRSKTTPLPSAVTAF 63
Query: 78 -SSLPMNIPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYLARTRGA---SLSVH 133
SSLP+NIP Y ++E SDD+ G +PPH + R + SV
Sbjct: 64 SSSLPVNIP------MRRYSTEEEYSDDDG-----GRKMIPPHLIVGRRMEGGQMAFSVC 112
Query: 134 EGKGRTLKGRDLRSVRNAIWKKVGF 158
G GRTLKGRDL VRN++ K GF
Sbjct: 113 TGNGRTLKGRDLSRVRNSVLKLTGF 137
>At2g34340 unknown protein
Length = 136
Score = 58.9 bits (141), Expect = 1e-09
Identities = 44/133 (33%), Positives = 69/133 (51%), Gaps = 9/133 (6%)
Query: 32 EFDEADVWNMSYSNSNTNIEPKKGVPGL---KRVSRKMEANNKVNPLASSSLPMNIPDWS 88
EFDE++V +S + E + + G ++ +R+++ SSSLP++IP+
Sbjct: 4 EFDESEVVFSDGLSSVLHKENENRLFGSDMERKKTRRIKRTTTEKASLSSSLPVSIPE-- 61
Query: 89 KILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYLART-RGASL--SVHEGKGRTLKGRDL 145
+ Y K+E E+E G +PPH + R +G + SV G GRTLKGRDL
Sbjct: 62 -NIFRRYVGKEEDEYSEEEYSEGGGEMIPPHIMIGRRIQGGQMAFSVCSGSGRTLKGRDL 120
Query: 146 RSVRNAIWKKVGF 158
VRN++ + GF
Sbjct: 121 SRVRNSVLRLTGF 133
>At2g16640 putative chloroplast outer membrane protein
Length = 1206
Score = 35.0 bits (79), Expect = 0.017
Identities = 30/101 (29%), Positives = 52/101 (50%), Gaps = 17/101 (16%)
Query: 44 SNSNTNIEPKKGVPGLKRVSRKMEANNKVNPLAS--SSLPMNIPDWSKILKEEYKKKK-- 99
+ +N ++ + +PG R A +K PL SSL + P K+ +++Y ++
Sbjct: 785 AEANALLKLQDNIPG-----RPFAARSKAPPLPFLLSSLLQSRPQ-PKLPEQQYGDEEDE 838
Query: 100 ----ESSDDEDEGDYDGVVQLPPHEYLARTRGASLSVHEGK 136
ESSD ++E +YD QLPP + L + + A+LS + K
Sbjct: 839 DDLEESSDSDEESEYD---QLPPFKSLTKAQMATLSKSQKK 876
>At5g45630 putative protein
Length = 114
Score = 32.3 bits (72), Expect = 0.11
Identities = 30/130 (23%), Positives = 54/130 (41%), Gaps = 25/130 (19%)
Query: 32 EFDEADVWNMSYSNSNTNIEPKKGVPGL---KRVSRKMEANNKVNPLASSSLPMNIPDWS 88
EF E+D+ S +T+ K K+ +R+ E K +P+ + +W
Sbjct: 4 EFQESDIIFSDQSKISTSSRYTKLYNSRNDEKKGTRRHETAEKTSPVRIPTNNFRCLEW- 62
Query: 89 KILKEEYKKKKESSDDEDEGDYDGVVQLPPHEYLARTRGASLSVHEGKGRTLKGRDLRSV 148
+++++ED+ + PPH + R ++ TLKGRDL
Sbjct: 63 -----------DTTEEEDD-------KTPPHVIIERRMKEQIAF---SACTLKGRDLSRH 101
Query: 149 RNAIWKKVGF 158
RN++ + GF
Sbjct: 102 RNSVLRMTGF 111
>At3g16620 putative GTP-binding protein
Length = 1089
Score = 32.0 bits (71), Expect = 0.15
Identities = 23/69 (33%), Positives = 36/69 (51%), Gaps = 10/69 (14%)
Query: 74 PLASSSLPMNIPDWSKILKEEYKKKK------ESSDDEDEGDYDGVVQLPPHEYLARTRG 127
PL SSL + P +K+ +++Y + ESSD E+E +YD +LPP + L +
Sbjct: 694 PLLLSSLLQSRPQ-AKLPEQQYDDEDDEDDLDESSDSEEESEYD---ELPPFKRLTKAEM 749
Query: 128 ASLSVHEGK 136
LS + K
Sbjct: 750 TKLSKSQKK 758
>At5g10070 unknown protein
Length = 264
Score = 30.0 bits (66), Expect = 0.56
Identities = 17/37 (45%), Positives = 20/37 (53%), Gaps = 3/37 (8%)
Query: 84 IPDWSKILKEEYKKKKESSDDEDEGDYDGVVQLPPHE 120
IP L+E KK ES D+ +E D DG LPP E
Sbjct: 214 IPKQPPALEEHVKKDGESGDESEEDDEDG---LPPLE 247
>At5g54460 unknown protein
Length = 141
Score = 29.6 bits (65), Expect = 0.73
Identities = 18/57 (31%), Positives = 33/57 (57%), Gaps = 4/57 (7%)
Query: 51 EPKKGVPGLKRVSRKMEANNKVNPLASSSLPMNIPDWSKILKEEYKKKKESSDDEDE 107
E KG LK+ +K E NK N S P+ + ++ +KEE +K+++ +++E+E
Sbjct: 81 EKYKGATRLKKEKKKWERKNKRN---QSKSPVK-EEGAEPVKEEKEKEEQGTENEEE 133
>At5g04240 zinc finger protein - like
Length = 1327
Score = 29.3 bits (64), Expect = 0.95
Identities = 18/69 (26%), Positives = 34/69 (49%)
Query: 12 TSYIFPETNFNQKSSQGKELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRVSRKMEANNK 71
+S I +N ++ SS G ++ DE + + S+ NT + G + RK+E+ ++
Sbjct: 1084 SSDILSSSNGDEASSNGLQVLNDELSMESEVSSSENTEVIEAPNSMGEAKKKRKIESESE 1143
Query: 72 VNPLASSSL 80
N SS+
Sbjct: 1144 TNDNPESSI 1152
>At3g27530 unknown protein
Length = 938
Score = 29.3 bits (64), Expect = 0.95
Identities = 23/89 (25%), Positives = 42/89 (46%), Gaps = 6/89 (6%)
Query: 23 QKSSQGKE-LEFDEADVWNMSYSNSNTNIEPKKGVPGLKRVSRKMEANN-----KVNPLA 76
Q++SQ E ++ ++A + + + SN N + + + L +E N +V L
Sbjct: 801 QETSQRLETVKAEKAKIESEASSNKNMAAKLEFDLKSLSDAYNSLEQANYHLEQEVKSLK 860
Query: 77 SSSLPMNIPDWSKILKEEYKKKKESSDDE 105
PM PD I +E K+ ++ S+DE
Sbjct: 861 GGESPMQFPDIEAIKEEVRKEAQKESEDE 889
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.308 0.128 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,977,083
Number of Sequences: 26719
Number of extensions: 178195
Number of successful extensions: 823
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 760
Number of HSP's gapped (non-prelim): 65
length of query: 160
length of database: 11,318,596
effective HSP length: 91
effective length of query: 69
effective length of database: 8,887,167
effective search space: 613214523
effective search space used: 613214523
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148995.13