BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148995.1 + phase: 0
(220 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g37210 unknown protein 370 e-103
At3g53450 putative protein 364 e-101
At2g28305 unknown protein 356 5e-99
At5g06300 lysine decarboxylase-like protein 328 1e-90
At5g11950 lysine decarboxylase - like protein 288 2e-78
At5g03270 lysine decarboxylase - like protein 271 3e-73
At4g35190 putative protein 266 5e-72
At2g35990 unknown protein 236 5e-63
At5g26140 putative protein 159 1e-39
At4g28510 prohibitin-like protein 29 1.7
At3g45850 kinesin-related protein - like 29 1.7
At1g75180 unknown protein 29 2.2
At4g28010 unknown protein 28 3.8
At5g11240 unknown protein 28 5.0
At1g55860 ubiquitin-protein ligase 1, putative 28 5.0
At5g10010 unknown protein 27 6.5
At2g18580 Ac-like transposase 27 6.5
At4g27920 unknown protein 27 8.5
>At2g37210 unknown protein
Length = 215
Score = 370 bits (951), Expect = e-103
Identities = 181/208 (87%), Positives = 197/208 (94%)
Query: 1 MDSRNGEIKLSKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGL 60
M+ + ++ SKF+RICVFCGSS GKK++YQDAA++LGNELVSRNIDLVYGGGSIGLMGL
Sbjct: 1 MEIKGESMQKSKFRRICVFCGSSQGKKSSYQDAAVDLGNELVSRNIDLVYGGGSIGLMGL 60
Query: 61 VSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGY 120
VSQAVHDGGRHVIG+IPKTLMPRELTGETVGEV+AVADMHQRKAEMAKHSDAFIALPGGY
Sbjct: 61 VSQAVHDGGRHVIGIIPKTLMPRELTGETVGEVRAVADMHQRKAEMAKHSDAFIALPGGY 120
Query: 121 GTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPT 180
GTLEELLEVITWAQLGIHDKPVGL+NVDGY+NSLLSFIDKAVEEGFISP AR IIVSAPT
Sbjct: 121 GTLEELLEVITWAQLGIHDKPVGLLNVDGYYNSLLSFIDKAVEEGFISPTAREIIVSAPT 180
Query: 181 AKELVKKLEEYVPCHEGVASKLSWQMEQ 208
AKELVKKLEEY PCHE VA+KL W+ME+
Sbjct: 181 AKELVKKLEEYAPCHERVATKLCWEMER 208
>At3g53450 putative protein
Length = 215
Score = 364 bits (935), Expect = e-101
Identities = 179/208 (86%), Positives = 195/208 (93%)
Query: 1 MDSRNGEIKLSKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGL 60
M+ N ++ SKF RICVFCGSS GKK++YQDAA++LGNELV RNIDLVYGGGSIGLMGL
Sbjct: 1 MEVNNETMQKSKFGRICVFCGSSQGKKSSYQDAAVDLGNELVLRNIDLVYGGGSIGLMGL 60
Query: 61 VSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGY 120
VSQAVHDGGRHVIGVIPKTLMPRELTGETVGEV+AVADMHQRKAEMA+HSDAFIALPGGY
Sbjct: 61 VSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVRAVADMHQRKAEMARHSDAFIALPGGY 120
Query: 121 GTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPT 180
GTLEELLEVITWAQLGIHDKPVGL+NVDGY+NSLLSFIDKAVEEGFIS NAR II+SAPT
Sbjct: 121 GTLEELLEVITWAQLGIHDKPVGLLNVDGYYNSLLSFIDKAVEEGFISTNARQIIISAPT 180
Query: 181 AKELVKKLEEYVPCHEGVASKLSWQMEQ 208
AKELVKKLEEY PCHE VA+KL W++E+
Sbjct: 181 AKELVKKLEEYSPCHESVATKLCWEIER 208
>At2g28305 unknown protein
Length = 213
Score = 356 bits (914), Expect = 5e-99
Identities = 176/198 (88%), Positives = 188/198 (94%)
Query: 11 SKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGR 70
SKFKRICVFCGSS G K +Y+DAA+ LG ELVSRNIDLVYGGGSIGLMGL+SQAV +GGR
Sbjct: 5 SKFKRICVFCGSSAGNKVSYKDAAIELGTELVSRNIDLVYGGGSIGLMGLISQAVFNGGR 64
Query: 71 HVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVI 130
HVIGVIPKTLMPRE+TGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVI
Sbjct: 65 HVIGVIPKTLMPREITGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVI 124
Query: 131 TWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEE 190
TWAQLGIHDKPVGL+NV+GY+NSLLSFIDKAVEEGFISP ARHIIVSAP+AKELVKKLE+
Sbjct: 125 TWAQLGIHDKPVGLLNVEGYYNSLLSFIDKAVEEGFISPTARHIIVSAPSAKELVKKLED 184
Query: 191 YVPCHEGVASKLSWQMEQ 208
YVP HE VASK SW+MEQ
Sbjct: 185 YVPRHEKVASKKSWEMEQ 202
>At5g06300 lysine decarboxylase-like protein
Length = 217
Score = 328 bits (842), Expect = 1e-90
Identities = 160/202 (79%), Positives = 181/202 (89%)
Query: 11 SKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGR 70
S+FKRICVFCGSS GKK +YQ+AA+ LGNELV R IDLVYGGGS+GLMGLVSQAVH GGR
Sbjct: 6 SRFKRICVFCGSSSGKKPSYQEAAIQLGNELVERRIDLVYGGGSVGLMGLVSQAVHHGGR 65
Query: 71 HVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVI 130
HV+GVIPKTLMPRE+TGET+GEVKAVADMHQRKAEMA+ +DAFIALPGGYGTLEELLEVI
Sbjct: 66 HVLGVIPKTLMPREITGETIGEVKAVADMHQRKAEMARQADAFIALPGGYGTLEELLEVI 125
Query: 131 TWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEE 190
TWAQLGIH KPVGL+NVDGY+NSLL+FIDKAV+EGFISP AR IIVSAP AKELV++LEE
Sbjct: 126 TWAQLGIHRKPVGLLNVDGYYNSLLTFIDKAVDEGFISPMARRIIVSAPNAKELVRQLEE 185
Query: 191 YVPCHEGVASKLSWQMEQQLAY 212
Y P + + SKL W +++Y
Sbjct: 186 YEPEFDEITSKLVWDEVDRISY 207
>At5g11950 lysine decarboxylase - like protein
Length = 216
Score = 288 bits (737), Expect = 2e-78
Identities = 134/203 (66%), Positives = 170/203 (83%)
Query: 11 SKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGR 70
S+F++ICVFCGS G + + DAA+ LGNELV R IDLVYGGGS+GLMGL+S+ V++GG
Sbjct: 7 SRFRKICVFCGSHSGHREVFSDAAIELGNELVKRKIDLVYGGGSVGLMGLISRRVYEGGL 66
Query: 71 HVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVI 130
HV+G+IPK LMP E++GETVG+V+ VADMH+RKA MA+ ++AFIALPGGYGT+EELLE+I
Sbjct: 67 HVLGIIPKALMPIEISGETVGDVRVVADMHERKAAMAQEAEAFIALPGGYGTMEELLEMI 126
Query: 131 TWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEE 190
TW+QLGIH K VGL+NVDGY+N+LL+ D VEEGFI P AR+I+VSAPTAKEL++K+EE
Sbjct: 127 TWSQLGIHKKTVGLLNVDGYYNNLLALFDTGVEEGFIKPGARNIVVSAPTAKELMEKMEE 186
Query: 191 YVPCHEGVASKLSWQMEQQLAYP 213
Y P H VAS SW++E+ YP
Sbjct: 187 YTPSHMHVASHESWKVEELGDYP 209
>At5g03270 lysine decarboxylase - like protein
Length = 229
Score = 271 bits (692), Expect = 3e-73
Identities = 141/190 (74%), Positives = 153/190 (80%), Gaps = 11/190 (5%)
Query: 11 SKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGR 70
S+FK ICVFCGSS G K +YQDAA++L ELV R IDLVYGGGSIGLMGLVSQAVHDGGR
Sbjct: 16 SRFKSICVFCGSSNGNKASYQDAAIDLAKELVMRKIDLVYGGGSIGLMGLVSQAVHDGGR 75
Query: 71 H-----------VIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGG 119
H + + +LTGETVGEVK VADMHQRKA MAKHSDAFI LPGG
Sbjct: 76 HNNNNNGNDDALFCHSVNVSQTNSKLTGETVGEVKEVADMHQRKAVMAKHSDAFITLPGG 135
Query: 120 YGTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAP 179
YGTLEELLEVITWAQLGIHDKPVGL+NVDGY+++LL FIDKAVEEGFI P ARHIIVSAP
Sbjct: 136 YGTLEELLEVITWAQLGIHDKPVGLLNVDGYYDALLLFIDKAVEEGFILPTARHIIVSAP 195
Query: 180 TAKELVKKLE 189
TA+EL KLE
Sbjct: 196 TARELFIKLE 205
>At4g35190 putative protein
Length = 268
Score = 266 bits (681), Expect = 5e-72
Identities = 142/248 (57%), Positives = 170/248 (68%), Gaps = 40/248 (16%)
Query: 7 EIKLSKFKRICVFCGSSPGKKTTYQDAAMNLGNELV----------------SRNIDLVY 50
EI S+FKR+CVFCGSS GK+ Y DAA +L ELV +R ++LVY
Sbjct: 2 EIVKSRFKRVCVFCGSSSGKRECYSDAATDLAQELVRLCLNLNESLENLKWVTRRLNLVY 61
Query: 51 GGGSIGLMGLVSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHS 110
GGGSIGLMGLVSQAVH+ G HV+G + +TGET GEV AVADMH+RKAEMA+HS
Sbjct: 62 GGGSIGLMGLVSQAVHEAGGHVLGYAQIYDLFTLITGETYGEVIAVADMHERKAEMARHS 121
Query: 111 DAFIALPGGYGTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPN 170
D FIALPGGYGTLEELLEVI WAQLGIHDKPVGL+NVDGY+N LL+FIDKAV++GFI P+
Sbjct: 122 DCFIALPGGYGTLEELLEVIAWAQLGIHDKPVGLLNVDGYYNYLLTFIDKAVDDGFIKPS 181
Query: 171 ARHIIVSAPTAKELVKKLE------------------------EYVPCHEGVASKLSWQM 206
RHI VSAP AKELV+KLE Y P ++GV +K W++
Sbjct: 182 QRHIFVSAPNAKELVQKLEILMKINKDKKFDSAIDLLFFDIPQAYKPVNDGVIAKSRWEV 241
Query: 207 EQQLAYPQ 214
E+++ PQ
Sbjct: 242 EKKVQQPQ 249
>At2g35990 unknown protein
Length = 181
Score = 236 bits (603), Expect = 5e-63
Identities = 118/182 (64%), Positives = 141/182 (76%), Gaps = 21/182 (11%)
Query: 11 SKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGR 70
S+F+RICVFCGSS G KTTY DAA+ L ++LV RNIDLVYGGGS+GLMGL+SQAVHDGGR
Sbjct: 6 SRFRRICVFCGSSSGNKTTYHDAALQLAHQLVERNIDLVYGGGSVGLMGLISQAVHDGGR 65
Query: 71 HVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVI 130
VI V+ MHQRKAEM + +DAFIALPGGYGT EELLEVI
Sbjct: 66 EVI---------------------TVSTMHQRKAEMGRQADAFIALPGGYGTFEELLEVI 104
Query: 131 TWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEE 190
TW+QLGIH KPVGL+NVDG+++SLL+FIDKAV+EGF+S AR IIVSAP A +L++ LE
Sbjct: 105 TWSQLGIHTKPVGLLNVDGFYDSLLTFIDKAVDEGFVSSTARRIIVSAPNAPQLLQLLEV 164
Query: 191 YV 192
++
Sbjct: 165 FL 166
>At5g26140 putative protein
Length = 143
Score = 159 bits (401), Expect = 1e-39
Identities = 79/130 (60%), Positives = 103/130 (78%), Gaps = 1/130 (0%)
Query: 85 LTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGG-YGTLEELLEVITWAQLGIHDKPVG 143
++GETVGEV+ V+DMH+RKA MA+ + AFIAL G Y T+EELLE+ITWAQLGIH K VG
Sbjct: 6 ISGETVGEVRIVSDMHERKATMAQEAGAFIALLGERYETMEELLEMITWAQLGIHKKTVG 65
Query: 144 LVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEEYVPCHEGVASKLS 203
L+NVDGY+N+LL+F D VEEGFI A +I+VSAP+A+EL++K+E Y P H+ +AS S
Sbjct: 66 LLNVDGYYNNLLAFFDTGVEEGFIKQGACNIVVSAPSARELMEKMELYTPSHKYIASHQS 125
Query: 204 WQMEQQLAYP 213
W++E YP
Sbjct: 126 WKVEPLGDYP 135
>At4g28510 prohibitin-like protein
Length = 288
Score = 29.3 bits (64), Expect = 1.7
Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 6/55 (10%)
Query: 116 LPGGYGTLEELLEVITWAQLGIHDKPVGLVNVDG-----YFNSLLSFIDKAVEEG 165
+PGG G + LL+V LG++ L NV+G FN L+ DK EG
Sbjct: 9 IPGG-GAISTLLKVGIIGGLGLYGATHSLYNVEGGHRAIMFNRLVGIKDKVYPEG 62
>At3g45850 kinesin-related protein - like
Length = 1058
Score = 29.3 bits (64), Expect = 1.7
Identities = 26/110 (23%), Positives = 43/110 (38%), Gaps = 3/110 (2%)
Query: 30 YQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVIGVIPKTLMPRELT-GE 88
+++ A N +L+ + +L+ + LV AVHD R TL T +
Sbjct: 756 FEECAANEERQLLEKVAELL-ANSNARKKNLVQMAVHDL-RESASTRTTTLQHEMSTMQD 813
Query: 89 TVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWAQLGIH 138
+ +KA +H K E + H D G E LL + ++ H
Sbjct: 814 STSSIKAEWSIHMEKTESSHHEDTSAVESGKKAMQEVLLNCLEKTEMSAH 863
>At1g75180 unknown protein
Length = 315
Score = 28.9 bits (63), Expect = 2.2
Identities = 20/73 (27%), Positives = 37/73 (50%), Gaps = 7/73 (9%)
Query: 145 VNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEEYVPCHEGVASKLSW 204
V V G F+ + K ++ GF + R TA + + + E P H+G +S+L +
Sbjct: 186 VYVTGCFSGTPIILTKTLQLGFRKKHGR------VTALDSIPEYETDEP-HKGNSSELDY 238
Query: 205 QMEQQLAYPQDYD 217
Q+ + +Y Q+Y+
Sbjct: 239 QVTEYGSYKQEYE 251
>At4g28010 unknown protein
Length = 704
Score = 28.1 bits (61), Expect = 3.8
Identities = 20/49 (40%), Positives = 26/49 (52%), Gaps = 10/49 (20%)
Query: 149 GYFNSLLSFIDKAVEEGFISPNA-------RHIIVSAPTAK--ELVKKL 188
GY + +SF DK V+ GF P+A ++ I T K ELVKKL
Sbjct: 613 GYLDEAISFFDKMVDSGF-EPDAHICDSVLKYCISQGETDKLTELVKKL 660
>At5g11240 unknown protein
Length = 615
Score = 27.7 bits (60), Expect = 5.0
Identities = 22/86 (25%), Positives = 39/86 (44%), Gaps = 9/86 (10%)
Query: 27 KTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVIGVIPK-TLMPREL 85
K ++Q + GN DLV G++ ++Q+V + GV K T + R
Sbjct: 334 KPSFQKMVLQFGN-------DLVLNASKDGILLPITQSVSKSSKRQ-GVQNKVTTLDRAH 385
Query: 86 TGETVGEVKAVADMHQRKAEMAKHSD 111
+ + + VAD+H++K+ SD
Sbjct: 386 AEDALLPIARVADLHEKKSVQLHSSD 411
>At1g55860 ubiquitin-protein ligase 1, putative
Length = 3891
Score = 27.7 bits (60), Expect = 5.0
Identities = 14/59 (23%), Positives = 28/59 (46%)
Query: 57 LMGLVSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIA 115
+MGL+ V+ + G P + +P +L + VGE + ++E+ +D +A
Sbjct: 3166 VMGLLQVVVYTAASRIEGWSPSSGVPEKLENKPVGEEASSETRKDAESELVGEADLSVA 3224
>At5g10010 unknown protein
Length = 273
Score = 27.3 bits (59), Expect = 6.5
Identities = 15/41 (36%), Positives = 21/41 (50%)
Query: 124 EELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEE 164
E+ LE T Q+ +P + D F+ L F+DK VEE
Sbjct: 147 EDELEQSTEVQIMFPSEPPVVCEFDWEFDELQEFVDKLVEE 187
>At2g18580 Ac-like transposase
Length = 308
Score = 27.3 bits (59), Expect = 6.5
Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 17/70 (24%)
Query: 135 LGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEEYVPC 194
+GIH K +++V +NS + KA++ KE+ + L E P
Sbjct: 9 VGIHTKAGLILDVTTRWNSTYLMLSKAIQ-----------------FKEVSRNLSELEPS 51
Query: 195 HEGVASKLSW 204
++ SKL W
Sbjct: 52 YKSFPSKLEW 61
>At4g27920 unknown protein
Length = 183
Score = 26.9 bits (58), Expect = 8.5
Identities = 17/57 (29%), Positives = 27/57 (46%), Gaps = 8/57 (14%)
Query: 160 KAVEEGFISPNARHIIVSAPTAKELVKKLEEYVPCHEGVASKLSWQMEQQLAYPQDY 216
K VE +I + RH +V + + LVK ++ P H L W + ++ PQ Y
Sbjct: 7 KKVESEYIKKHHRHELVESQCSSTLVKHIK--APLH------LVWSIVRRFDEPQKY 55
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.136 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,086,869
Number of Sequences: 26719
Number of extensions: 216071
Number of successful extensions: 506
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 488
Number of HSP's gapped (non-prelim): 18
length of query: 220
length of database: 11,318,596
effective HSP length: 95
effective length of query: 125
effective length of database: 8,780,291
effective search space: 1097536375
effective search space used: 1097536375
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148995.1