BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148969.6 + phase: 0
(280 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g15390 peptide deformylase-like protein 243 6e-65
At5g14660 unknown protein 89 3e-18
At1g76620 unknown protein 35 0.034
At4g12290 copper amine oxidase -like protein 32 0.50
At1g55960 putative membrane related protein CP5 (At1g55960) 31 0.65
At3g55270 MAP kinase phosphatase (MKP1) 30 1.4
At3g30190 hypothetical protein 30 1.4
At4g20950 putative protein 30 1.9
At5g45770 predicted GPI-anchored protein 29 3.2
At5g22660 unknown protein 29 3.2
At2g40400 unknown protein 28 4.2
At1g36760 hypothetical protein 28 4.2
At1g34355 orkhead-associated domain-containing protein / FHA 28 4.2
At5g39785 unknown protein 28 5.5
At2g28890 unknown protein 28 5.5
At2g04810 hypothetical protein 28 5.5
At1g07630 unknown protein 28 5.5
At4g26860 unknown protein 28 7.2
At3g50930 BCS1 protein-like protein 28 7.2
At3g24630 hypothetical protein 28 7.2
>At1g15390 peptide deformylase-like protein
Length = 269
Score = 243 bits (621), Expect = 6e-65
Identities = 137/252 (54%), Positives = 172/252 (67%), Gaps = 6/252 (2%)
Query: 13 TEMEAARRLASRLRVVPMMLVFSNAT--VSSSSSSCNTETPPSNTKLSSFSSASSETAFL 70
T ME R++ RL V + + VS SS N SS SS S++ +L
Sbjct: 9 TAMETLFRVSLRLLPVSAAVTCRSIRFPVSRPGSSHLLNRKLYNLPTSSSSSLSTKAGWL 68
Query: 71 ----SKTLKSELPHIVQAGDPVLHEPAREVDHSEINSDKIQKIIDGMILVMRNAPGISLS 126
K K +LP IV +GDPVLHE AREVD EI S++IQKIID MI VMR APG+ L+
Sbjct: 69 LGLGEKKKKVDLPEIVASGDPVLHEKAREVDPGEIGSERIQKIIDDMIKVMRLAPGVGLA 128
Query: 127 AQKIGIPLRIIVLEEPKENLYNYTEEVNKIIDRRPFDLLVILNPKLKIKSNKTFLFFEGC 186
A +IG+PLRIIVLE+ KE + +E +RR FDL+V++NP LK +SNK LFFEGC
Sbjct: 129 APQIGVPLRIIVLEDTKEYISYAPKEEILAQERRHFDLMVMVNPVLKERSNKKALFFEGC 188
Query: 187 LSVHGFQAVVERYLDVEVEGFDRYGEPIKINASGWHARILQHECDHLDGTLYVDKMVPRT 246
LSV GF+A VERYL+V V G+DR G+ I++NASGW ARILQHECDHLDG LYVDKMVPRT
Sbjct: 189 LSVDGFRAAVERYLEVVVTGYDRQGKRIEVNASGWQARILQHECDHLDGNLYVDKMVPRT 248
Query: 247 FRSWENINMSIA 258
FR+ +N+++ +A
Sbjct: 249 FRTVDNLDLPLA 260
>At5g14660 unknown protein
Length = 273
Score = 88.6 bits (218), Expect = 3e-18
Identities = 58/172 (33%), Positives = 87/172 (49%), Gaps = 23/172 (13%)
Query: 81 IVQAGDPVLHEPAREVDHSEINSDKIQKIIDGMILVMRNAPGISLSAQKIGIPLRIIVLE 140
IV+ DP+L + +D I + ++ ++D M VM GI LSA ++G+ ++++V
Sbjct: 83 IVEYPDPILRAKNKRID---IFDENLKNLVDAMFDVMYKTDGIGLSAPQVGLNVQLMVFN 139
Query: 141 ---EPKENLYNYTEEVNKIIDRRPFDLLVILNPKLKIKSNKTFLFFEGCLSVHGFQAVVE 197
EP E +V++NPK+K S+K F EGCLS G A V
Sbjct: 140 PAGEPGEGKE-----------------IVLVNPKIKKYSDKLVPFDEGCLSFPGIYAEVV 182
Query: 198 RYLDVEVEGFDRYGEPIKINASGWHARILQHECDHLDGTLYVDKMVPRTFRS 249
R V+++ D GE I+ S ARI QHE DHL+G L+ D+M + S
Sbjct: 183 RPQSVKIDARDITGERFSISLSRLPARIFQHEYDHLEGVLFFDRMTDQVLDS 234
>At1g76620 unknown protein
Length = 527
Score = 35.4 bits (80), Expect = 0.034
Identities = 17/44 (38%), Positives = 26/44 (58%)
Query: 31 MLVFSNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTL 74
M+ N SSSSSS +T +P S++ SS+S + E+ FL +
Sbjct: 1 MMALMNCAYSSSSSSSSTSSPTSSSSSSSYSPRNRESMFLQSVV 44
>At4g12290 copper amine oxidase -like protein
Length = 756
Score = 31.6 bits (70), Expect = 0.50
Identities = 35/143 (24%), Positives = 55/143 (37%), Gaps = 26/143 (18%)
Query: 32 LVFSNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTL--KSELPHIVQAGDP-- 87
L+F T S +T P + T+L + +SS S+ + P + DP
Sbjct: 24 LIFLIVTAGFIISFTSTNFPHAPTRLLDCTDSSSSPLCASRNFLFNKQQPRPIPKHDPKP 83
Query: 88 ----------VLHEPAREVDHSEINSDKIQKIIDGMILVMRNAPGISLSAQKIGIPLRII 137
+ P + SEIN KI+ I+ L P L +
Sbjct: 84 NTKNHDHVSDTPNHPLDPLTVSEIN--KIRSILSSHALFTSGTPHA----------LHTV 131
Query: 138 VLEEPKENLYNYTEEVNKIIDRR 160
VLEEP++NL + E+ N + R+
Sbjct: 132 VLEEPEKNLVRHWEKGNPLPPRK 154
>At1g55960 putative membrane related protein CP5 (At1g55960)
Length = 403
Score = 31.2 bits (69), Expect = 0.65
Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 1/66 (1%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSE-TAFLSKTLKSELPHIVQAGDPVLHEPA 93
S+++ SSSSSS +TE+ P+N++ S E FL + L+ E + + V+H+
Sbjct: 55 SSSSSSSSSSSPSTESVPANSQSRISKLVSDEDLKFLIENLEEETNDSTEIWEHVIHKSN 114
Query: 94 REVDHS 99
+ +S
Sbjct: 115 DRISYS 120
>At3g55270 MAP kinase phosphatase (MKP1)
Length = 732
Score = 30.0 bits (66), Expect = 1.4
Identities = 20/58 (34%), Positives = 34/58 (58%), Gaps = 2/58 (3%)
Query: 23 SRLRVVPMMLVFSNATVSSSSSSCNTETPP--SNTKLSSFSSASSETAFLSKTLKSEL 78
SR+ MM+V S+ + SS++SS +T +PP S + S +S +S +F + +S L
Sbjct: 425 SRVYSDSMMIVHSSGSPSSTTSSSSTASPPFLSPDSVCSTNSGNSLKSFSQSSGRSSL 482
>At3g30190 hypothetical protein
Length = 263
Score = 30.0 bits (66), Expect = 1.4
Identities = 16/37 (43%), Positives = 27/37 (72%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLS 71
S+++ SSSSSS ++ + S++ SS SS+SS ++F S
Sbjct: 32 SSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSSSSFSS 68
Score = 27.7 bits (60), Expect = 7.2
Identities = 15/42 (35%), Positives = 28/42 (65%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKS 76
S+++ SSSSSS ++ + S++ SS SS+S+ ++ S + S
Sbjct: 27 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSSSSFSS 68
Score = 27.7 bits (60), Expect = 7.2
Identities = 16/41 (39%), Positives = 25/41 (60%)
Query: 36 NATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKS 76
N + SSSSSS ++ + S++ SS SS+SS + S + S
Sbjct: 25 NGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSSSS 65
Score = 27.7 bits (60), Expect = 7.2
Identities = 18/48 (37%), Positives = 31/48 (64%), Gaps = 1/48 (2%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELPHIV 82
S+++ SSSSSS ++ + S++ SS SS+SS +++ L L H+V
Sbjct: 35 SSSSSSSSSSSSSSSSSSSSSSTSSSSSSSSFSSYFVVPLLRYL-HVV 81
Score = 27.3 bits (59), Expect = 9.4
Identities = 15/39 (38%), Positives = 26/39 (66%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKT 73
+ ++ SSSSSS ++ + S++ SS SS+SS T+ S +
Sbjct: 25 NGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSS 63
>At4g20950 putative protein
Length = 264
Score = 29.6 bits (65), Expect = 1.9
Identities = 28/88 (31%), Positives = 41/88 (45%), Gaps = 11/88 (12%)
Query: 6 GSSSLFETEMEAARRLASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSFSSASS 65
G S + + ++L S L V + + +SSSSSS + P KL S SS+SS
Sbjct: 10 GKSGIENWGLALLKKLVSSLLFVKLF----RSPLSSSSSSARPQAPQLVIKLLSSSSSSS 65
Query: 66 ET-----AFLSKTL--KSELPHIVQAGD 86
+S +L K+ LP + AGD
Sbjct: 66 SARPTPRLAISSSLDCKTHLPPRLAAGD 93
>At5g45770 predicted GPI-anchored protein
Length = 425
Score = 28.9 bits (63), Expect = 3.2
Identities = 16/41 (39%), Positives = 24/41 (58%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLK 75
+N SSSS +C+ +P + SF++ SS + SKTLK
Sbjct: 52 NNNQSSSSSITCDDASPYRHITSISFTNCSSTLSLPSKTLK 92
>At5g22660 unknown protein
Length = 315
Score = 28.9 bits (63), Expect = 3.2
Identities = 24/91 (26%), Positives = 41/91 (44%), Gaps = 22/91 (24%)
Query: 94 REVDHSEINSDKIQKIIDGMILVMRNAPGISLSAQKIGIPLRIIVLEE------------ 141
R + H E++S +I +ID + L + LS + + L +++L
Sbjct: 111 RRIQHLEVDS-RIDHMIDTLPLT------VYLSESLVSLRLHLVMLHRFVFVSLPNLKVM 163
Query: 142 -PKENLYNYTEEVNKIIDRRPF--DLLVILN 169
+EN+Y+Y E + K I P DL V+ N
Sbjct: 164 HLEENIYSYAETMEKFISSCPVLEDLTVVRN 194
>At2g40400 unknown protein
Length = 735
Score = 28.5 bits (62), Expect = 4.2
Identities = 25/100 (25%), Positives = 44/100 (44%), Gaps = 8/100 (8%)
Query: 42 SSSSCNTETPPSNTK-LSSFSSASSETAFLSKTLKSELPHIVQAGDPVLHEPAREVDHSE 100
S S TPP+ + +S F+S S ++ L +P L AR V+
Sbjct: 267 SESERKLYTPPAGSGFISGFTSFSRSSSLNMNPLTQIVPF---GPSSYLSAQARVVEDHT 323
Query: 101 INSDKIQKIIDG----MILVMRNAPGISLSAQKIGIPLRI 136
++ +Q + DG M++V+ A + ++ G+P RI
Sbjct: 324 MSQVIVQAVADGGGTGMLVVVTGANHVEYGSRGTGLPARI 363
>At1g36760 hypothetical protein
Length = 104
Score = 28.5 bits (62), Expect = 4.2
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 3/77 (3%)
Query: 7 SSSLFETEMEAARRLASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSFSSASSE 66
S +L + A+ ++ + V+ + + S V ++ +C+T+ PSN+ S SS+S+
Sbjct: 23 SETLISKKSLASHKVVAWTNVIKGV-IRSKTEVMTAKEACSTQARPSNS-TSRPSSSSTH 80
Query: 67 TAFLSKTLKSELPHIVQ 83
T S T K +L H+ Q
Sbjct: 81 TKCSSSTSKLDL-HVKQ 96
>At1g34355 orkhead-associated domain-containing protein / FHA
Length = 1477
Score = 28.5 bits (62), Expect = 4.2
Identities = 15/70 (21%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Query: 35 SNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELPHIVQAGDPVLHEPAR 94
S+ S C++ PPS ++++ + E+ +S+T K + ++ +G +
Sbjct: 862 SSINTDDIQSLCSSSQPPSESEVNPATDQDQESGIISETEKPKTELLIGSGRSEKYYSLS 921
Query: 95 EVDHSEINSD 104
E++ E N+D
Sbjct: 922 EIEGEE-NTD 930
>At5g39785 unknown protein
Length = 606
Score = 28.1 bits (61), Expect = 5.5
Identities = 20/65 (30%), Positives = 29/65 (43%)
Query: 19 RRLASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSEL 78
R +L ++ ++ + S S T T SN +SFSS S L K KSE+
Sbjct: 277 RERMRKLDILSFQKSYALGLLQSKSPQQATSTLGSNPSQTSFSSVFSVNIRLWKAKKSEI 336
Query: 79 PHIVQ 83
+VQ
Sbjct: 337 EPMVQ 341
>At2g28890 unknown protein
Length = 654
Score = 28.1 bits (61), Expect = 5.5
Identities = 15/42 (35%), Positives = 20/42 (46%)
Query: 38 TVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELP 79
T+S +S S NT TP S + + AF S T S +P
Sbjct: 66 TISGASVSANTATPLSTSLYDPYGHIDRAAAFESTTSFSSIP 107
>At2g04810 hypothetical protein
Length = 397
Score = 28.1 bits (61), Expect = 5.5
Identities = 15/49 (30%), Positives = 31/49 (62%), Gaps = 8/49 (16%)
Query: 143 KENLY--NYTEEVN------KIIDRRPFDLLVILNPKLKIKSNKTFLFF 183
++NLY N+ E+ + KI+ RR ++L++L+ K K+++ + LF+
Sbjct: 251 EQNLYPFNFVEDPSEYVWKRKIVIRRSGEVLIVLSLKKKVQNEEKLLFY 299
>At1g07630 unknown protein
Length = 662
Score = 28.1 bits (61), Expect = 5.5
Identities = 15/42 (35%), Positives = 20/42 (46%)
Query: 38 TVSSSSSSCNTETPPSNTKLSSFSSASSETAFLSKTLKSELP 79
T+S +S S NT TP S + + AF S T S +P
Sbjct: 69 TISGASVSANTATPLSTSLYDPYGHIDRAAAFESTTSFSSIP 110
>At4g26860 unknown protein
Length = 244
Score = 27.7 bits (60), Expect = 7.2
Identities = 39/155 (25%), Positives = 61/155 (39%), Gaps = 27/155 (17%)
Query: 148 NYTEEVNKIIDRRPFDLLVILNPKLKIKSNKTFLFFEGCLS-VHGFQAV-VERYLDVEVE 205
NY +E+ + P D+ L+ KT L L+ VHG V +LD V
Sbjct: 63 NYVQEIIDKAPQLPEDIEWHFVGHLQSNKAKTLLTGVPNLAMVHGVDGEKVANHLDRAVS 122
Query: 206 GFDRYGEPIKI----NASGWH-------------ARILQHECDHL--DGTLYVDK----M 242
R+ P+K+ N SG AR ++H C +L G + +
Sbjct: 123 NLGRH--PLKVLVQVNTSGEVSKSGIEPSSVVELARHVKHHCPNLVFSGLMTIGMPDYTS 180
Query: 243 VPRTFRSWENINMSIARAVGVSNLAAELKTGAAFD 277
P FR+ N + +A+G++ EL G + D
Sbjct: 181 TPENFRTLSNCRADVCKALGMAEDQFELSMGMSGD 215
>At3g50930 BCS1 protein-like protein
Length = 576
Score = 27.7 bits (60), Expect = 7.2
Identities = 11/48 (22%), Positives = 28/48 (57%)
Query: 71 SKTLKSELPHIVQAGDPVLHEPAREVDHSEINSDKIQKIIDGMILVMR 118
+KT K EL + + + +EVD + +D++ K+++G++ +++
Sbjct: 510 AKTEKQELENKKKTKEGTDSVVKKEVDEQLVRNDRVDKVLEGLVELLK 557
>At3g24630 hypothetical protein
Length = 724
Score = 27.7 bits (60), Expect = 7.2
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 4/78 (5%)
Query: 1 MRKLQGSSSLFETEMEAARRLASRLRVVPMMLVFSNATVSSSSSSCNTETPPSNTKLSSF 60
+RK+ S L + + + ++ RVV S+ VSSS+SS + +TK
Sbjct: 182 LRKVIRESFLRQNLVSQTTTIETKKRVVRSDFASSSGAVSSSTSSSQSSMVSGSTK---- 237
Query: 61 SSASSETAFLSKTLKSEL 78
SSASS+ + +L + L
Sbjct: 238 SSASSDVPQRAPSLIARL 255
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.133 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,817,289
Number of Sequences: 26719
Number of extensions: 239351
Number of successful extensions: 1037
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1002
Number of HSP's gapped (non-prelim): 30
length of query: 280
length of database: 11,318,596
effective HSP length: 98
effective length of query: 182
effective length of database: 8,700,134
effective search space: 1583424388
effective search space used: 1583424388
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148969.6