BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148819.5 - phase: 0
(83 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45520 copia-like polyprotein 42 7e-05
At2g07550 putative retroelement pol polyprotein 37 0.002
At4g21360 putative transposable element 36 0.003
At5g35820 copia-like retrotransposable element 35 0.005
At4g10460 putative retrotransposon 35 0.007
At2g21460 putative retroelement pol polyprotein 33 0.025
At3g28160 non-LTR reverse transcriptase, putative 32 0.043
At2g21310 putative retroelement pol polyprotein 32 0.043
At1g37110 32 0.073
At4g04280 putative transposon protein 30 0.21
At4g04600 putative polyprotein 30 0.28
At1g49900 Cys2/His2-type zinc finger protein, putative 27 1.8
At1g42560 membrane protein Mlo9 27 2.4
At2g15700 copia-like retroelement pol polyprotein 26 3.1
At4g08100 putative polyprotein 26 4.0
At2g33670 similar to Mlo proteins from H. vulgare 25 5.2
At4g29510 arginine methyltransferase (pam1) 25 9.0
>At3g45520 copia-like polyprotein
Length = 1363
Score = 41.6 bits (96), Expect = 7e-05
Identities = 28/97 (28%), Positives = 47/97 (47%), Gaps = 15/97 (15%)
Query: 2 GTKWDIEKFIGDSDFGLWKVK---------IEVVLIQQKCAKARKGKILFSDTMSQEDKT 52
G + ++EKF G D+ +WK K + VL + + ++ SD +E++
Sbjct: 3 GARIEVEKFDGRGDYTMWKEKLLAHIDMLGLSAVLRESETPMGKERDSEKSDEDEKEERE 62
Query: 53 KM------ADNARSFFVLCLRDKVLREVVKDTTIKVM 83
KM ARS VL + D+VLR++ K+T+ M
Sbjct: 63 KMEAFEEKKRKARSTIVLSVSDRVLRKIKKETSAAAM 99
>At2g07550 putative retroelement pol polyprotein
Length = 1356
Score = 36.6 bits (83), Expect = 0.002
Identities = 24/93 (25%), Positives = 44/93 (46%), Gaps = 15/93 (16%)
Query: 6 DIEKFIGDSDFGLWKVKIEVVL--------IQQKCAKARKGKILFSDTMSQEDKTKMAD- 56
++EKF G D+ +WK K+ + +++ + K +L E+K + +
Sbjct: 7 EVEKFDGRGDYTMWKEKLLAHMDILGLNTALKESESTGEKKSVLDESDEDYEEKLEKFEA 66
Query: 57 ------NARSFFVLCLRDKVLREVVKDTTIKVM 83
ARS VL + D+VLR++ K++T M
Sbjct: 67 LEEKKKKARSAIVLSVTDRVLRKIKKESTAAAM 99
>At4g21360 putative transposable element
Length = 1308
Score = 36.2 bits (82), Expect = 0.003
Identities = 26/98 (26%), Positives = 45/98 (45%), Gaps = 24/98 (24%)
Query: 1 MGTKWDIEKFIGDSDFGLWKVKIE-------------------VVLIQQKCAKARKGKIL 41
M TK +I+ F GD DF LWK++IE +L+ + K + +
Sbjct: 3 MSTKVEIKTFNGDRDFSLWKIRIEAQLGVLGLKPALSDFTLTKTILVVKSEKKESESEDD 62
Query: 42 FSDTMSQED-----KTKMADNARSFFVLCLRDKVLREV 74
+D+ E+ K + +D A++F + + D VL +V
Sbjct: 63 ETDSKKTEEVPDPIKFEQSDQAKNFIINHITDTVLLKV 100
>At5g35820 copia-like retrotransposable element
Length = 1342
Score = 35.4 bits (80), Expect = 0.005
Identities = 30/94 (31%), Positives = 47/94 (49%), Gaps = 20/94 (21%)
Query: 6 DIEKFIGDSDFGLWKVKI----EVV-----LIQQKCAKARKGKILFSDTMSQEDKT---K 53
++EKF GD D+ LWK K+ E++ L +++ A SD +Q+ +T K
Sbjct: 7 EVEKFDGDGDYILWKEKLLAHMEMLGLLEGLGEEEEAVVEDSTTEISDGGNQDPETATSK 66
Query: 54 MAD--------NARSFFVLCLRDKVLREVVKDTT 79
+ D ARS +L L + VLR+V+K T
Sbjct: 67 LEDKILKEKRGKARSTIILSLGNNVLRKVIKQKT 100
>At4g10460 putative retrotransposon
Length = 1230
Score = 35.0 bits (79), Expect = 0.007
Identities = 26/91 (28%), Positives = 41/91 (44%), Gaps = 13/91 (14%)
Query: 6 DIEKFIGDSDFGLWKVKIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMAD--------- 56
++EKF G D+ LWK K+ + A + SD + E++ K ++
Sbjct: 7 EMEKFDGHGDYTLWKEKLMAHMDLLGLTVALRETQSVSDPLESEEEGKESEKGDKEALME 66
Query: 57 ----NARSFFVLCLRDKVLREVVKDTTIKVM 83
ARS VL + D+VLR+ K+ T M
Sbjct: 67 EKRQKARSTIVLSVSDQVLRKSKKEKTAPSM 97
>At2g21460 putative retroelement pol polyprotein
Length = 1333
Score = 33.1 bits (74), Expect = 0.025
Identities = 24/98 (24%), Positives = 43/98 (43%), Gaps = 25/98 (25%)
Query: 6 DIEKFIGDSDFGLWKVK--------------------IEVVLIQQKCAKARKGKILFSDT 45
++EKF G D+ +WK K +E V Q + K ++L +
Sbjct: 7 EVEKFDGRGDYTMWKEKLMAHLDILGLSVALKEEDDLVEKVAEMQLTEEEEKEEVLRREL 66
Query: 46 MSQEDKTKMADNARSFFVLCLRDKVLREVVKDTTIKVM 83
+ ++ + ARS VL + D+VLR++ K+ + M
Sbjct: 67 LEEKRR-----KARSAIVLSVTDRVLRKIKKEQSAAAM 99
>At3g28160 non-LTR reverse transcriptase, putative
Length = 630
Score = 32.3 bits (72), Expect = 0.043
Identities = 24/88 (27%), Positives = 41/88 (46%), Gaps = 14/88 (15%)
Query: 6 DIEKFIGDSDFGLWKVKIEVVLIQQKCAKARKGKILF----SDTMSQEDKTKMAD----- 56
D+EKF G D+ WKVK L + K ++ ++ S E + K +
Sbjct: 7 DLEKFDGFGDYISWKVKFLDHLKDLDLLEGLKEEVCLEKGSGNSGSDEGRVKDLEECKIL 66
Query: 57 -----NARSFFVLCLRDKVLREVVKDTT 79
AR+F ++ + D VLR+++K+ T
Sbjct: 67 EEKKRKARAFIIVSVGDHVLRKIMKERT 94
>At2g21310 putative retroelement pol polyprotein
Length = 838
Score = 32.3 bits (72), Expect = 0.043
Identities = 26/90 (28%), Positives = 42/90 (45%), Gaps = 20/90 (22%)
Query: 6 DIEKFIGDSDFGLWKVKI--------------EVVLIQQKCAKARKGKILFSDTMSQEDK 51
++EK G+ D+ LWK K+ E I+++ + A +L EDK
Sbjct: 7 EVEKLDGEGDYVLWKEKLLAHIELLGLLEGLEEDEAIEEEESTAETDSLL----TKTEDK 62
Query: 52 T--KMADNARSFFVLCLRDKVLREVVKDTT 79
+ ARS +L L + VLR+V+K+ T
Sbjct: 63 VLKEKRGKARSTVILSLGNHVLRKVIKEKT 92
>At1g37110
Length = 1356
Score = 31.6 bits (70), Expect = 0.073
Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 10/58 (17%)
Query: 3 TKWDIEKFIGDSDFGLWKVKIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMADNARS 60
T+ +I+ F GD DF LWK++I+ A+ G + DT++ TK +S
Sbjct: 6 TRVEIKVFNGDRDFSLWKIRIQ----------AQLGVLGLKDTLTDFSLTKTVPLTKS 53
>At4g04280 putative transposon protein
Length = 1104
Score = 30.0 bits (66), Expect = 0.21
Identities = 12/24 (50%), Positives = 16/24 (66%)
Query: 4 KWDIEKFIGDSDFGLWKVKIEVVL 27
K +I+ F GD DF WK++IE L
Sbjct: 2 KVEIKTFNGDRDFSFWKIRIEAQL 25
>At4g04600 putative polyprotein
Length = 922
Score = 29.6 bits (65), Expect = 0.28
Identities = 11/21 (52%), Positives = 17/21 (80%)
Query: 3 TKWDIEKFIGDSDFGLWKVKI 23
T+ +I+ F GD+DF LWK++I
Sbjct: 5 TRVEIKAFDGDNDFSLWKMRI 25
>At1g49900 Cys2/His2-type zinc finger protein, putative
Length = 917
Score = 26.9 bits (58), Expect = 1.8
Identities = 13/51 (25%), Positives = 29/51 (56%)
Query: 6 DIEKFIGDSDFGLWKVKIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMAD 56
D++ +G+SD G + EVV+ + KC+ + G I+ + + ++ ++ D
Sbjct: 339 DLKLSLGESDGGHKDSQDEVVVHEDKCSPSSNGSIVTNVSDPEQSVRRLID 389
>At1g42560 membrane protein Mlo9
Length = 460
Score = 26.6 bits (57), Expect = 2.4
Identities = 18/71 (25%), Positives = 32/71 (44%), Gaps = 1/71 (1%)
Query: 7 IEKFIGDSDFGLWKV-KIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMADNARSFFVLC 65
I +G + WKV + EV+ Q+ + ++ + +E A N F+V+C
Sbjct: 174 ITMMLGRAKIRGWKVWEQEVIHEQEMMNDPSRFRLTHETSFVREHVNSWASNKFFFYVMC 233
Query: 66 LRDKVLREVVK 76
++LR V K
Sbjct: 234 FFRQILRSVRK 244
>At2g15700 copia-like retroelement pol polyprotein
Length = 1166
Score = 26.2 bits (56), Expect = 3.1
Identities = 8/18 (44%), Positives = 14/18 (77%)
Query: 6 DIEKFIGDSDFGLWKVKI 23
++++F G DF LWKV++
Sbjct: 8 EVDRFDGTGDFSLWKVRM 25
>At4g08100 putative polyprotein
Length = 1054
Score = 25.8 bits (55), Expect = 4.0
Identities = 10/22 (45%), Positives = 16/22 (72%)
Query: 19 WKVKIEVVLIQQKCAKARKGKI 40
W+ KIE+V + Q+C ++ K KI
Sbjct: 111 WEQKIELVFLCQECLQSNKVKI 132
>At2g33670 similar to Mlo proteins from H. vulgare
Length = 501
Score = 25.4 bits (54), Expect = 5.2
Identities = 18/71 (25%), Positives = 32/71 (44%), Gaps = 1/71 (1%)
Query: 7 IEKFIGDSDFGLWKV-KIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMADNARSFFVLC 65
I +G + WKV + EV+ + + ++ + +E A N SF+V+C
Sbjct: 167 ITMMLGRAKIRGWKVWEEEVINDHEMMNDPSRFRLTHETSFVREHVNPWAKNRFSFYVMC 226
Query: 66 LRDKVLREVVK 76
++LR V K
Sbjct: 227 FFRQMLRSVRK 237
>At4g29510 arginine methyltransferase (pam1)
Length = 390
Score = 24.6 bits (52), Expect = 9.0
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Query: 19 WKVKIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMADNARSFFVLCLRDKVLREVVKDT 78
W V+ VVL KA D+ +EDK + ++ F + C++ K + E + DT
Sbjct: 203 WLVEGGVVLPD----KASLHLTAIEDSEYKEDKIEFWNSVYGFDMSCIKKKAMMEPLVDT 258
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.323 0.137 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,691,876
Number of Sequences: 26719
Number of extensions: 51268
Number of successful extensions: 171
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 151
Number of HSP's gapped (non-prelim): 17
length of query: 83
length of database: 11,318,596
effective HSP length: 59
effective length of query: 24
effective length of database: 9,742,175
effective search space: 233812200
effective search space used: 233812200
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 52 (24.6 bits)
Medicago: description of AC148819.5