BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148532.5 + phase: 0 /pseudo
(343 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g25610 dehydration-induced protein RD22 243 8e-65
At1g49320 unknown protein 148 4e-36
At1g60390 122 3e-28
At1g70370 unknown protein 120 1e-27
At1g23760 putative polygalacuronase isoenzyme 1 beta subunit 115 3e-26
At3g14010 unknown protein 32 0.50
At3g06160 hypothetical protein 30 2.5
At1g68800 hypothetical protein 28 5.6
At5g16720 putative protein 28 7.3
At3g47890 putative protein 28 7.3
>At5g25610 dehydration-induced protein RD22
Length = 392
Score = 243 bits (621), Expect = 8e-65
Identities = 160/396 (40%), Positives = 203/396 (50%), Gaps = 82/396 (20%)
Query: 10 LVATHATLPPELYWKSKLPTTQIPKAITDILHPIC*LNLYRLYNFIFTLMKG-------- 61
+VA A L PE YW + LP T IP + L+ L F FT K
Sbjct: 16 VVAIAADLTPERYWSTALPNTPIPNS------------LHNLLTFDFTDEKSTNVQVGKG 63
Query: 62 -------KAKCDSKICM**LT*KLIQLGVTSVDDGK--------ISVD------------ 94
K K S + + G VD GK +SV
Sbjct: 64 GVNVNTHKGKTGSGTAV------NVGKGGVRVDTGKGKPGGGTHVSVGSGKGHGGGVAVH 117
Query: 95 ------------GKISVNGKTRIVYDAAPIYF----------YENDANEAELHDNRNLAM 132
GK V TR + PIY Y A E +LHD+ N A+
Sbjct: 118 TGKPGKRTDVGVGKGGVTVHTR--HKGRPIYVGVKPGANPFVYNYAAKETQLHDDPNAAL 175
Query: 133 FLLEKDLHHGTKFNIQFTKTSDHG--PTFLPGDVANSIPFSSNKLENILNYFSIKQGSTE 190
F LEKDL G + N++F +G FLP A ++PF S K L FS++ GS E
Sbjct: 176 FFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEE 235
Query: 191 SEIVKNTISECEAYGIKGEEKLCVTSLESMIDFTTLKLGN-NVDTVSTEVNGESG-LQQY 248
+E++K TI ECEA + GEEK C TSLESM+DF+ KLG +V VSTEV ++ +Q+Y
Sbjct: 236 AEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKY 295
Query: 249 VIAN-GVKKMGENNLVVCHKRNYPYAVFYCHKTDATKVYSVPLEGADGSRVKAVAICHSD 307
IA GVKK+ ++ VVCHK+ YP+AVFYCHK T VY+VPLEG +G R KAVA+CH +
Sbjct: 296 KIAAAGVKKLSDDKSVVCHKQKYPFAVFYCHKAMMTTVYAVPLEGENGMRAKAVAVCHKN 355
Query: 308 TSQWSLKHLAFQVLKVQQGTFPVCHILQQGQVVWFS 343
TS W+ HLAF+VLKV+ GT PVCH L + VVWFS
Sbjct: 356 TSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVWFS 391
>At1g49320 unknown protein
Length = 280
Score = 148 bits (374), Expect = 4e-36
Identities = 85/227 (37%), Positives = 122/227 (53%), Gaps = 11/227 (4%)
Query: 126 DNRNLAMFLLEKDLHHGTKFNIQFTKTS-DHGPTFLPGDVANSIPFSSNKLENILNYFSI 184
D+ +L M+ DL GTK I F K P L A+ IPF+ +KL+ +L++FSI
Sbjct: 51 DDPSLYMYFTLNDLKLGTKLLIYFYKNDLQKLPPLLTRQQADLIPFTKSKLDFLLDHFSI 110
Query: 185 KQGSTESEIVKNTISECEAYGIKGEEKLCVTSLESMIDFTTLKLGNNVD----TVSTEVN 240
+ S + + +K T+ C+A I+GE K C TSLES+ID +G NVD T V
Sbjct: 111 TKDSPQGKAIKETLGHCDAKAIEGEHKFCGTSLESLIDLVKKTMGYNVDLKVMTTKVMVP 170
Query: 241 GES----GLQQYVIANGVKKMGENNLVVCHKRNYPYAVFYCH-KTDATKVYSVPLEGADG 295
++ L Y K++ ++ CH+ YPYAV+YCH ++V+ V L DG
Sbjct: 171 AQNSISYALHNYTFVEAPKELVGIKMLGCHRMPYPYAVYYCHGHKGGSRVFEVNLVTDDG 230
Query: 296 -SRVKAVAICHSDTSQWSLKHLAFQVLKVQQGTFPVCHILQQGQVVW 341
RV A+CH DTS W H+AF+VLK++ + PVCH +VW
Sbjct: 231 RQRVVGPAVCHMDTSTWDADHVAFKVLKMEPRSAPVCHFFPLDNIVW 277
>At1g60390
Length = 624
Score = 122 bits (306), Expect = 3e-28
Identities = 86/265 (32%), Positives = 119/265 (44%), Gaps = 13/265 (4%)
Query: 89 GKISVDGKISVN----GKTRIVYDAAPIYF--YENDANEAELHDNRNL--AMFLLEKDLH 140
G+ +V G +S G++ VY + F Y N+ + N+ + F E L
Sbjct: 357 GQDNVGGNVSFKTYGQGQSFKVYTKDGVVFARYSNNVSSNGKTVNKWVEEGKFFREAMLK 416
Query: 141 HGTKFNIQFTKTSDHGPTFLPGDVANSIPFSSNKLENILNYFSIKQGSTESEIVKNTISE 200
GT + K TFLP ++ ++PFSS+ + I F + S+ + I+ + +SE
Sbjct: 417 EGTLMQMPDIKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSE 476
Query: 201 CEAYGIKGEEKLCVTSLESMIDFTTLKLGNNVDTVSTEVNGESGLQQYVI--ANGVKKMG 258
CE GE K CV S E MIDF T LG V V T N ++ VI NG+
Sbjct: 477 CERPASHGETKRCVGSAEDMIDFATSVLGRGV-VVRTTENVVGSKKKVVIGKVNGINGGD 535
Query: 259 ENNLVVCHKRNYPYAVFYCHKTDATKVYSVPLEGADGSRV--KAVAICHSDTSQWSLKHL 316
V CH+ YPY ++YCH +VY L VAICH DTS WS H
Sbjct: 536 VTRAVSCHQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHG 595
Query: 317 AFQVLKVQQGTFPVCHILQQGQVVW 341
AF L G VCH + + + W
Sbjct: 596 AFLALGSGPGQIEVCHWIFENDMTW 620
>At1g70370 unknown protein
Length = 626
Score = 120 bits (301), Expect = 1e-27
Identities = 73/213 (34%), Positives = 100/213 (46%), Gaps = 5/213 (2%)
Query: 133 FLLEKDLHHGTKFNIQFTKTSDHGPTFLPGDVANSIPFSSNKLENILNYFSIKQGSTESE 192
F E L GT + K +FLP + +PFS++KL I F + ST
Sbjct: 411 FFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGG 470
Query: 193 IVKNTISECEAYGIKGEEKLCVTSLESMIDFTTLKLGNNVDTVSTEVNGESGLQQYVI-- 250
I+ + ++ECE GE K CV S E MIDF T LG +V +TE N ++ VI
Sbjct: 471 IITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTE-NVAGSKEKVVIGK 529
Query: 251 ANGVKKMGENNLVVCHKRNYPYAVFYCHKTDATKVYSVPLEGADGSRV--KAVAICHSDT 308
NG+ V CH+ YPY ++YCH +VY L + + +AICH DT
Sbjct: 530 VNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDT 589
Query: 309 SQWSLKHLAFQVLKVQQGTFPVCHILQQGQVVW 341
S W H AF L + G VCH + + + W
Sbjct: 590 SSWGPSHGAFLALGSKPGRIEVCHWIFENDMNW 622
>At1g23760 putative polygalacuronase isoenzyme 1 beta subunit
Length = 622
Score = 115 bits (289), Expect = 3e-26
Identities = 71/212 (33%), Positives = 99/212 (46%), Gaps = 3/212 (1%)
Query: 133 FLLEKDLHHGTKFNIQFTKTSDHGPTFLPGDVANSIPFSSNKLENILNYFSIKQGSTESE 192
F E L GT + K +FLP + + +PFS++K+ I F ST
Sbjct: 407 FFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEG 466
Query: 193 IVKNTISECEAYGIKGEEKLCVTSLESMIDFTTLKLGNNVDTVSTE-VNGESGLQQYVIA 251
I+ + + ECE E K CV S E MIDF T LG +V +TE V G
Sbjct: 467 IITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKV 526
Query: 252 NGVKKMGENNLVVCHKRNYPYAVFYCHKTDATKVY-SVPLEGADGSRVK-AVAICHSDTS 309
NG+ V CH+ YPY ++YCH +VY S L+ +++ +AICH DTS
Sbjct: 527 NGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTS 586
Query: 310 QWSLKHLAFQVLKVQQGTFPVCHILQQGQVVW 341
W H AF +L + G VCH + + + W
Sbjct: 587 AWGANHGAFMLLGSRPGQIEVCHWIFENDMNW 618
>At3g14010 unknown protein
Length = 595
Score = 32.0 bits (71), Expect = 0.50
Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Query: 180 NYFSIKQGSTESEIVKNTISECEAYGIKGEEKLCVTSLESMIDFTTLKLGNNVD 233
N F +K+G TE + K S G G K SL+ ++ FTT K+G++V+
Sbjct: 11 NGFPLKRGETEEVLHKTNTSNTVFNGEAGSLKRL--SLDRLVYFTTCKIGHHVE 62
>At3g06160 hypothetical protein
Length = 330
Score = 29.6 bits (65), Expect = 2.5
Identities = 34/125 (27%), Positives = 55/125 (43%), Gaps = 13/125 (10%)
Query: 83 VTSVDDGKISVDGKISVNG---KTRIVYDAAPIYFYENDANEAELHDNRNLAMFLLEKDL 139
+T V DG S + I G +TR V I D N LH N+++ L D
Sbjct: 98 LTFVFDGHKSYEVSIYGRGDCKETRAVIQVEEISDDTEDDN-VSLHSPSNVSLDSLSNDS 156
Query: 140 HHGTKFNIQFTKTSD---HGPTFLPGDVANS---IPFSSNKLEN--ILNYFSIKQGSTES 191
HH T N+ S+ HG + D S +P +S +E+ ++N + +Q S +
Sbjct: 157 HHSTS-NVSLRSLSNDSLHGDAEIESDSEYSPENLPSASISVESVEVVNPTTSRQRSYKR 215
Query: 192 EIVKN 196
+ ++N
Sbjct: 216 KTIEN 220
>At1g68800 hypothetical protein
Length = 317
Score = 28.5 bits (62), Expect = 5.6
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 5/53 (9%)
Query: 167 SIPFSSNKLEN-----ILNYFSIKQGSTESEIVKNTISECEAYGIKGEEKLCV 214
S PFSS+ LE+ ++N F ++Q + +V++ C+ +K + + CV
Sbjct: 22 SFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCV 74
>At5g16720 putative protein
Length = 600
Score = 28.1 bits (61), Expect = 7.3
Identities = 21/88 (23%), Positives = 38/88 (42%), Gaps = 2/88 (2%)
Query: 153 SDHGPTFLPGDVANSIPFSSNKLENILNYFSIKQGSTESEIVKNTISECEAYGIKGEEKL 212
S+ G LP + S KLE I S+ + E E + C + KG++
Sbjct: 511 SEDGSQGLPESDEKNFGSDSEKLEIIKQVDSVYERLQELETDGEFLKNCMSSAKKGDKGT 570
Query: 213 CVTS--LESMIDFTTLKLGNNVDTVSTE 238
+ L+ + D T++L N ++ +T+
Sbjct: 571 DILKDILQHLRDLRTIELTNTIENQTTQ 598
>At3g47890 putative protein
Length = 1528
Score = 28.1 bits (61), Expect = 7.3
Identities = 11/30 (36%), Positives = 19/30 (62%)
Query: 174 KLENILNYFSIKQGSTESEIVKNTISECEA 203
KL ++ ++FS + E+EI+ +S CEA
Sbjct: 306 KLSDLKSHFSASKDGNENEIISEALSFCEA 335
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.326 0.140 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,529,335
Number of Sequences: 26719
Number of extensions: 310154
Number of successful extensions: 863
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 845
Number of HSP's gapped (non-prelim): 11
length of query: 343
length of database: 11,318,596
effective HSP length: 100
effective length of query: 243
effective length of database: 8,646,696
effective search space: 2101147128
effective search space used: 2101147128
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC148532.5