BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148236.4 - phase: 0
(279 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64460 putative phosphoglycerate mutase 376 e-105
At1g58280 putative protein 360 e-100
At2g17280 unknown protein 348 2e-96
At1g09935 putative protein 197 7e-51
At2g23210 putative glucosyltransferase 35 0.058
At3g60450 unknown protein 29 3.2
At2g06890 putative retroelement integrase 28 4.2
At3g60420 unknown protein 28 7.1
At4g24600 hypothetical protein 27 9.3
>At5g64460 putative phosphoglycerate mutase
Length = 282
Score = 376 bits (966), Expect = e-105
Identities = 179/256 (69%), Positives = 211/256 (81%)
Query: 11 QTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIEL 70
+T VRHAQG HNV+G+KN AY+S+D FDA LT LGWKQVD+LR+HV +SGL K+IEL
Sbjct: 16 KTIYLVRHAQGIHNVDGEKNYKAYMSHDYFDAELTQLGWKQVDSLRKHVHSSGLHKKIEL 75
Query: 71 VIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVELCRE 130
VI SPL+RT+QTAVGVFGGE +TD D PLM N NSSR AISSLN PP + E CRE
Sbjct: 76 VISSPLMRTLQTAVGVFGGEGYTDMSDVLPLMVANAGNSSRAAISSLNCPPVITEESCRE 135
Query: 131 HLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLKFLEWLWT 190
HLGVHPCD+RR IS+YQ +FPA+DFSLIE +ED LWK D+RE EE+AARG KFL WLWT
Sbjct: 136 HLGVHPCDQRRSISDYQFLFPAVDFSLIESEEDKLWKADVRETIEELAARGKKFLNWLWT 195
Query: 191 RKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDESS 250
RKEKEIA+V+HSGFLFHTL+A N+CH +VK EIC HFANCELRS+VI+DR +GSD S
Sbjct: 196 RKEKEIAIVTHSGFLFHTLNALQNECHPDVKKEICGHFANCELRSMVIVDRSMLGSDSSV 255
Query: 251 TNFPGKIPQGLDLPSD 266
T++PGKIP+G+DLPSD
Sbjct: 256 TDYPGKIPKGIDLPSD 271
>At1g58280 putative protein
Length = 276
Score = 360 bits (923), Expect = e-100
Identities = 172/261 (65%), Positives = 206/261 (78%)
Query: 11 QTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIEL 70
+T VRHAQG HNVEG+KN AYLS DLFDA LTPLGW+QVDNL +HV ASG+S RIEL
Sbjct: 16 KTIHLVRHAQGIHNVEGEKNHKAYLSEDLFDAHLTPLGWQQVDNLHKHVNASGISNRIEL 75
Query: 71 VIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVELCRE 130
V+VSPLLRT+QTAVG FGGE + DGV+ P LM NS R AIS LN PPF+AVE CRE
Sbjct: 76 VVVSPLLRTLQTAVGTFGGEGYKDGVNTPLLMTAGAGNSDRPAISRLNRPPFIAVESCRE 135
Query: 131 HLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLKFLEWLWT 190
HLGVHPCD+R I++Y+ +FPAIDFSLIE DED+LWKPDIRE+++++A RG+KF WL T
Sbjct: 136 HLGVHPCDRRSNITKYRELFPAIDFSLIETDEDVLWKPDIREEDKDIATRGVKFFNWLST 195
Query: 191 RKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDESS 250
RKEKEIAVV+HSGFL+ TL++FGNDC +VK+EI F NCELRS V++D+ SD
Sbjct: 196 RKEKEIAVVTHSGFLYQTLNSFGNDCDPSVKNEISKKFVNCELRSFVLVDKCMSSSDPPM 255
Query: 251 TNFPGKIPQGLDLPSDVADEK 271
TN+PG I G D SD+AD+K
Sbjct: 256 TNYPGTILTGEDASSDIADQK 276
>At2g17280 unknown protein
Length = 271
Score = 348 bits (893), Expect = 2e-96
Identities = 165/256 (64%), Positives = 206/256 (80%), Gaps = 1/256 (0%)
Query: 11 QTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIEL 70
+T VRHAQG HNV G+K+ AY S D FDA LTPLGW+QVDNLR HV+A+ L ++EL
Sbjct: 15 KTIHLVRHAQGIHNVAGEKDHSAYSSEDYFDAHLTPLGWQQVDNLRNHVRAAQLLNKVEL 74
Query: 71 VIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVELCRE 130
VIVSP+LRT+QTAVG FGGE T+G DA PLM N +S R AISSLNSPPF+AVELCRE
Sbjct: 75 VIVSPMLRTIQTAVGAFGGEEDTNGADATPLMVANAGSSDRPAISSLNSPPFLAVELCRE 134
Query: 131 HLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLKFLEWLWT 190
+G HPCD+RR ++EY+ +FPAIDFS+IE D D+LWKP RE EEVAARG++F++W+WT
Sbjct: 135 TMGDHPCDRRRSVTEYKALFPAIDFSIIETDNDVLWKPSPRESLEEVAARGVEFIKWIWT 194
Query: 191 RKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDES- 249
RKEKEIA+VSHSGFL LS+FG DC ++K E+ H +NCELRS+VI+DRG +G+D +
Sbjct: 195 RKEKEIAIVSHSGFLHGLLSSFGKDCCDDLKKELSIHLSNCELRSMVIVDRGNLGTDSAE 254
Query: 250 STNFPGKIPQGLDLPS 265
+TN+PGK+P+GLD PS
Sbjct: 255 TTNYPGKVPEGLDNPS 270
>At1g09935 putative protein
Length = 212
Score = 197 bits (500), Expect = 7e-51
Identities = 108/234 (46%), Positives = 140/234 (59%), Gaps = 39/234 (16%)
Query: 16 VRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIELVIVSP 75
+RH Q HNVE +K+ +A LS LFDA LT G +QV+NLR+ V +SGL KR+ELV+ SP
Sbjct: 18 LRHGQALHNVEAEKDRNALLSPHLFDAPLTDHGHQQVENLRERVVSSGLLKRVELVVTSP 77
Query: 76 LLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVELCREHLGVH 135
L RTMQTAVGVFG E S +SPP +A+E+ R+ GV
Sbjct: 78 LFRTMQTAVGVFGNEYKQS--------------------SMTSSPPILALEVARDRNGVR 117
Query: 136 PCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLKFLEWLWTRKEKE 195
P D RR IE +ED LW+PD+RE EE+ ARGL+F++W EKE
Sbjct: 118 PPDMRRN---------------IESEEDNLWRPDVRESEEEILARGLEFMKW----PEKE 158
Query: 196 IAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDES 249
+AVVSH L H L F NDC +++ E+C FANCE+R+VVI+D+G S E+
Sbjct: 159 VAVVSHGIVLQHMLYVFANDCDLSIRHELCKRFANCEIRTVVIVDKGMASSTEN 212
>At2g23210 putative glucosyltransferase
Length = 453
Score = 34.7 bits (78), Expect = 0.058
Identities = 26/71 (36%), Positives = 38/71 (52%), Gaps = 6/71 (8%)
Query: 168 PDIREKNEEVAARGLKFLEWLWTRKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTH 227
PD+ + N++V GL FLE R + + SH G +F+TL A +C +VK +
Sbjct: 150 PDLEDPNQKVELPGLPFLE---VRDLPTLMLPSH-GAIFNTLMAEFVECLKDVKWVLANS 205
Query: 228 FANCELRSVVI 238
F EL SV+I
Sbjct: 206 F--YELESVII 214
>At3g60450 unknown protein
Length = 274
Score = 28.9 bits (63), Expect = 3.2
Identities = 38/154 (24%), Positives = 69/154 (44%), Gaps = 10/154 (6%)
Query: 68 IELVIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENV--VNSSRHAIS-SLNSPPFVA 124
I V VSP LR +QTA V + + VD + +++V ++ S+ +S L +
Sbjct: 66 IHRVFVSPFLRCLQTASEVVAALSAVN-VDPNAMSSKDVPSIDKSKLKVSIELGLCEMLN 124
Query: 125 VELCREHLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWK--PDIREKNEEVAARGL 182
R L +S+ + MFP +++ + D+++K P E E R +
Sbjct: 125 SVAIRRELAPKDGKFDFTVSDIETMFPE---GMVDHNVDMVYKELPKWEESVEGCRDRYV 181
Query: 183 KFLEWLWTR-KEKEIAVVSHSGFLFHTLSAFGND 215
K ++ L + E+ + +V+H + T S F D
Sbjct: 182 KVVKALADKYPEENLLLVTHGEGVGTTFSTFYKD 215
>At2g06890 putative retroelement integrase
Length = 1215
Score = 28.5 bits (62), Expect = 4.2
Identities = 41/182 (22%), Positives = 64/182 (34%), Gaps = 18/182 (9%)
Query: 67 RIELVIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRH----AISSLNSPPF 122
+IE ++ +L Q ++ G A P SS H S P+
Sbjct: 78 QIEEMLHKAILFEQQVKRKSSSRSSYGSGTIAKPTYQREERTSSYHNKPIVSPRAESKPY 137
Query: 123 VAV-------ELCREHLGVHPCDKRRGISEYQNMFP------AIDFSLIERDEDILWKPD 169
AV E+ + C K +G Y N P +D IE +E+I P
Sbjct: 138 AAVQDHKGKAEISTSRVRDVRCYKCQGKGHYANECPNKRVMILLDNGEIEPEEEIPDSPS 197
Query: 170 IREKNEEVAARGLKFLEWLWTRKEKEIAVVSHSGFLFHT-LSAFGNDCHANVKSEICTHF 228
++NEE+ A+G + + + LFHT G C + CT+
Sbjct: 198 SLKENEELPAQGELLVARRTLSVQTKTDEQEQRKNLFHTRCHVHGKVCSLIIDGGSCTNV 257
Query: 229 AN 230
A+
Sbjct: 258 AS 259
>At3g60420 unknown protein
Length = 270
Score = 27.7 bits (60), Expect = 7.1
Identities = 43/155 (27%), Positives = 67/155 (42%), Gaps = 24/155 (15%)
Query: 62 SGLSKRIELVIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPP 121
S L I V VSP LR +QTA V A VD P + SS+ +S N+
Sbjct: 59 SQLGVPIHRVFVSPFLRCIQTASEVV---AALSAVDFDP-----IAMSSKDVLSIDNTKI 110
Query: 122 FVAVELCREHL--------GVHPCDKRRG--ISEYQNMFPAIDFSLIERDEDILWK--PD 169
VA+E + V P D + IS+ + MFP ++ + D+++K P+
Sbjct: 111 KVAIEFGLSEIPHPIFIKSEVAPKDGKFDFKISDLEAMFPE---GTVDSNVDMVYKEVPE 167
Query: 170 IREKNEEVAARGLKFLEWLWTRKEKE-IAVVSHSG 203
E + R K ++ L + E + +V+H G
Sbjct: 168 WGESAQAFEDRYYKTVKILAEKYPSENLLLVTHWG 202
>At4g24600 hypothetical protein
Length = 124
Score = 27.3 bits (59), Expect = 9.3
Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 1/35 (2%)
Query: 28 DKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKAS 62
+K+PD +S L+D TP+ QVDNL +++AS
Sbjct: 2 EKDPDFAVSL-LWDGENTPVEVDQVDNLINNIEAS 35
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,657,745
Number of Sequences: 26719
Number of extensions: 279216
Number of successful extensions: 592
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 583
Number of HSP's gapped (non-prelim): 11
length of query: 279
length of database: 11,318,596
effective HSP length: 98
effective length of query: 181
effective length of database: 8,700,134
effective search space: 1574724254
effective search space used: 1574724254
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148236.4