BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147538.3 - phase: 0
(123 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g27950 GPI-anchored protein (LTPL) 112 6e-26
At3g22600 predicted GPI-anchored protein 56 4e-09
At2g48130 predicted GPI-anchored protein 56 5e-09
At4g14815 Expressed protein 55 7e-09
At2g44290 non-specific lipid transfer protein (nLTP) like protein 55 7e-09
At1g55260 unknown protein 55 7e-09
At2g44300 predicted GPI-anchored protein 55 9e-09
At1g73890 predicted GPI-anchored protein 55 1e-08
At3g58550 predicted GPI-anchored protein 54 2e-08
At5g09370 putative lipid transfer protein, predicted GPI-anchore... 50 2e-07
At1g36150 hypothetical protein 48 1e-06
At5g13900 predicted GPI-anchored protein 47 2e-06
At3g43720 lipid-transfer protein-like, predicted GPI-anchored pr... 46 5e-06
At2g13820 predicted GPI-anchored protein 46 5e-06
At1g05450 hypothetical protein 43 3e-05
At1g18280 predicted GPI-anchored protein 43 4e-05
At1g73560 lipid transfer protein, putative 42 1e-04
At4g33355 non-specific lipid transfer protein 40 3e-04
At5g64080 predicted GPI-anchored protein 40 4e-04
At2g48140 unknown protein 39 8e-04
>At1g27950 GPI-anchored protein (LTPL)
Length = 193
Score = 112 bits (279), Expect = 6e-26
Identities = 52/111 (46%), Positives = 70/111 (62%), Gaps = 2/111 (1%)
Query: 6 HQMFMCLCVLALIIGGCNGAED--LASKCGSVVQKVIPCLDFATGKAPTPKKECCDAANS 63
H + + + ++A I A LA +C QKV CLDFATGKA P K+CCDA
Sbjct: 7 HLVLVTMTIVASIAAAAPAAPGGALADECNQDFQKVTLCLDFATGKATIPSKKCCDAVED 66
Query: 64 IKATDPECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVNGANISDCP 114
IK DP+CLC++IQQ G K +G+QEDKL+QLPT C ++ A+I++CP
Sbjct: 67 IKERDPKCLCFVIQQAKTGGQALKDLGVQEDKLIQLPTSCQLHNASITNCP 117
>At3g22600 predicted GPI-anchored protein
Length = 170
Score = 56.2 bits (134), Expect = 4e-09
Identities = 22/84 (26%), Positives = 44/84 (52%), Gaps = 4/84 (4%)
Query: 30 SKCGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPESKSM 89
S C + + + PCL++ TG + +P ++CC+ + + + P+CLC ++ G +
Sbjct: 26 SSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLCQVL----NGGGSQLGI 81
Query: 90 GIQEDKLLQLPTVCHVNGANISDC 113
+ + + L LP C+V +S C
Sbjct: 82 NVNQTQALGLPRACNVQTPPVSRC 105
>At2g48130 predicted GPI-anchored protein
Length = 183
Score = 55.8 bits (133), Expect = 5e-09
Identities = 25/86 (29%), Positives = 44/86 (51%), Gaps = 6/86 (6%)
Query: 29 ASKCGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSP-ESK 87
+S C S + + PCL + TG + TP + CC +S+ + P+C+C + SP +
Sbjct: 27 SSSCVSTLTTLSPCLSYITGNSTTPSQPCCSRLDSVIKSSPQCICSAV-----NSPIPNI 81
Query: 88 SMGIQEDKLLQLPTVCHVNGANISDC 113
+ I + LQLP C++ ++ C
Sbjct: 82 GLNINRTQALQLPNACNIQTPPLTQC 107
>At4g14815 Expressed protein
Length = 156
Score = 55.5 bits (132), Expect = 7e-09
Identities = 31/111 (27%), Positives = 47/111 (41%), Gaps = 5/111 (4%)
Query: 10 MCLCVLALIIGGCNGAEDLASKCGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKATDP 69
MCL +L + + S C +V+ + PCL F T P ++CC+ +
Sbjct: 5 MCL-ILFIALMRVMSIVSAQSSCTNVLISMAPCLSFITQNTSLPSQQCCNQLAHVVRYSS 63
Query: 70 ECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVNGANISDCPSKSKTD 120
ECLC ++ G + + E + L LP CHV S C S S +
Sbjct: 64 ECLCQVLD----GGGSQLGINVNETQALALPKACHVETPPASRCHSGSSVN 110
>At2g44290 non-specific lipid transfer protein (nLTP) like protein
Length = 205
Score = 55.5 bits (132), Expect = 7e-09
Identities = 31/108 (28%), Positives = 58/108 (53%), Gaps = 7/108 (6%)
Query: 10 MCLCVLALII--GGCNGAEDLASKCGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKAT 67
+ L ++A+++ G + +D +C + + + CL + GKA +P +CC + +
Sbjct: 13 IALIMVAMVVDAAGADKGKD-KEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINS 71
Query: 68 DPECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVNGANISDCPS 115
D +CLC IIQ+ + P+ + + L LP+VCH A+I+ CP+
Sbjct: 72 DMKCLCMIIQE--RNDPD-LGLQVNVSLALALPSVCHAT-ADITKCPA 115
>At1g55260 unknown protein
Length = 184
Score = 55.5 bits (132), Expect = 7e-09
Identities = 31/120 (25%), Positives = 61/120 (50%), Gaps = 12/120 (10%)
Query: 1 MKNQQHQMFMCLCVLALIIGGCNGAEDLAS---KCGSVVQKVIPCLDFATGKAPTPKKEC 57
M+ +F+ + + ++++G G DLA +C + + ++ C+ + G A P K+C
Sbjct: 1 MEKSTRTLFITIVITSMLLGF--GNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDC 58
Query: 58 CDAANSIKATDPECLCYIIQQTHKGSPESKSMGIQEDKLL--QLPTVCHVNGANISDCPS 115
C + +C+C +++ K P+ +GI+ + L LP+ CH+ NI+DC S
Sbjct: 59 CAGFGQVIRKSEKCVCILVRD--KDDPQ---LGIKINATLAAHLPSACHITAPNITDCIS 113
>At2g44300 predicted GPI-anchored protein
Length = 204
Score = 55.1 bits (131), Expect = 9e-09
Identities = 28/106 (26%), Positives = 53/106 (49%), Gaps = 4/106 (3%)
Query: 10 MCLCVLALIIGGCNGAEDLASKCGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKATDP 69
+ L V+A+++ + +C + + CL + G+A +P +CC + ++
Sbjct: 13 IALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNK 72
Query: 70 ECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVNGANISDCPS 115
+CLC IIQ + P+ + I L LP+VCH A+++ CP+
Sbjct: 73 KCLCVIIQD--RNDPD-LGLQINVSLALALPSVCHA-AADVTKCPA 114
>At1g73890 predicted GPI-anchored protein
Length = 193
Score = 54.7 bits (130), Expect = 1e-08
Identities = 30/84 (35%), Positives = 42/84 (49%), Gaps = 4/84 (4%)
Query: 32 CGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPESKSMGI 91
C S + + PC F G A P + CCD+ N I + + CLC + T SP + I
Sbjct: 30 CASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSP---AFPI 86
Query: 92 QEDKLLQLPTVCHVNGANISDCPS 115
+ LQLP +C++ AN S C S
Sbjct: 87 NQTLALQLPPLCNI-PANSSTCSS 109
>At3g58550 predicted GPI-anchored protein
Length = 177
Score = 53.5 bits (127), Expect = 2e-08
Identities = 34/117 (29%), Positives = 51/117 (43%), Gaps = 11/117 (9%)
Query: 3 NQQHQMF-MCLCVLALIIGGCNGAEDLASKCGSVVQKVIPCLDFATGKAPTPKKECCDA- 60
NQ QM +C+ V + +G + C + + CL F T KA P CC
Sbjct: 8 NQNPQMLALCITVAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTL 67
Query: 61 -ANSIKATDPECLCYIIQQTHKGSPESKSMGIQED--KLLQLPTVCHVNGANISDCP 114
K +CLC +++ + +G + D + + LP+ CHV ANIS CP
Sbjct: 68 KVKIDKGQTRKCLCTLVKDR-----DDPGLGFKVDANRAMSLPSACHV-PANISQCP 118
>At5g09370 putative lipid transfer protein, predicted GPI-anchored
protein
Length = 129
Score = 50.4 bits (119), Expect = 2e-07
Identities = 29/89 (32%), Positives = 42/89 (46%), Gaps = 8/89 (8%)
Query: 29 ASKCGSVVQKVIPCLDFAT--GKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPES 86
AS C ++V + PCL F + G A TP CC + +I T P CLC +++ G
Sbjct: 25 ASDCDTLVITLFPCLPFISIGGTADTPTASCCSSLKNILDTKPICLCEGLKKAPLG---- 80
Query: 87 KSMGIQEDKLLQLPTVCHVNGANISDCPS 115
+ + K LP C +N +S C S
Sbjct: 81 --IKLNVTKSATLPVACKLNAPPVSACDS 107
>At1g36150 hypothetical protein
Length = 256
Score = 48.1 bits (113), Expect = 1e-06
Identities = 24/81 (29%), Positives = 41/81 (49%), Gaps = 11/81 (13%)
Query: 29 ASKCGSVVQKVIPCLDFAT--GKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPES 86
++ C SV+ ++ CL F T P+P K CC ++ P+CLC ++ S
Sbjct: 42 STDCSSVIYSMVDCLSFLTVGSTDPSPTKTCCVGVKTVLNYSPKCLCSALE-------SS 94
Query: 87 KSMG--IQEDKLLQLPTVCHV 105
+ MG + + K L +P +C+V
Sbjct: 95 REMGFVLDDTKALAMPKICNV 115
>At5g13900 predicted GPI-anchored protein
Length = 151
Score = 47.0 bits (110), Expect = 2e-06
Identities = 28/100 (28%), Positives = 51/100 (51%), Gaps = 6/100 (6%)
Query: 9 FMCLCVLALIIGGCNGAEDLASKCGSVVQKVIPCLDFATGKAPTPKKECCDAANSIKATD 68
F V+ L++ +G + + S + ++ PCL++ G P+ CC+ S+ +
Sbjct: 7 FFSFYVVLLLVAASSGMR-INGQSVSCLNQLAPCLNYLNGTKEVPQV-CCNPLKSVIRNN 64
Query: 69 PECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVC--HVN 106
PECLC +I +++ S +++ GI + LP C HVN
Sbjct: 65 PECLCRMI--SNRWSSQAERAGIDVNDAQMLPARCGEHVN 102
>At3g43720 lipid-transfer protein-like, predicted GPI-anchored
protein
Length = 193
Score = 45.8 bits (107), Expect = 5e-06
Identities = 32/99 (32%), Positives = 47/99 (47%), Gaps = 15/99 (15%)
Query: 21 GCNGAEDLASKCGSVVQKVIPCLDFAT----GKAPTPKKECCDAANSIKATDPECLCYII 76
G +GA D C + + + CL + T G A P K CC A + + P+CLCY++
Sbjct: 31 GPSGAPD----CMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLL 86
Query: 77 QQTHKGSPESKSMGIQED--KLLQLPTVCHVNGANISDC 113
+ +GI+ D K L+LP VC V + S C
Sbjct: 87 -----SGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLC 120
>At2g13820 predicted GPI-anchored protein
Length = 169
Score = 45.8 bits (107), Expect = 5e-06
Identities = 34/110 (30%), Positives = 48/110 (42%), Gaps = 14/110 (12%)
Query: 8 MFMCLCVLALIIGGCNGAEDLASKCGSVVQKVIPCLDFATGKAPTPKKE--CCDAANSIK 65
+ M V+AL+ G A A C S++ + CL F T + K E CC ++
Sbjct: 6 ILMIFSVVALMSGERAHA---AVDCSSLILNMADCLSFVTSGSTVVKPEGTCCSGLKTVV 62
Query: 66 ATDPECLCYIIQQTHKGSPESKSMGIQED--KLLQLPTVCHVNGANISDC 113
T PECLC + S S+G+ D K LP+VC V + C
Sbjct: 63 RTGPECLC-------EAFKNSGSLGLTLDLSKAASLPSVCKVAAPPSARC 105
>At1g05450 hypothetical protein
Length = 206
Score = 43.1 bits (100), Expect = 3e-05
Identities = 24/77 (31%), Positives = 34/77 (43%), Gaps = 5/77 (6%)
Query: 34 SVVQKVIPCLDFATGKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPESKSMGIQE 93
S++ V C F TG P +CC A S+ T +CLC I+ G P S+ I
Sbjct: 36 SMLSSVTGCTSFLTGGGSFPTSDCCGALKSLTGTGMDCLCLIVT---AGVP--ISIPINR 90
Query: 94 DKLLQLPTVCHVNGANI 110
+ LP C + G +
Sbjct: 91 TLAISLPRACGIPGVPV 107
>At1g18280 predicted GPI-anchored protein
Length = 180
Score = 42.7 bits (99), Expect = 4e-05
Identities = 25/85 (29%), Positives = 39/85 (45%), Gaps = 6/85 (7%)
Query: 36 VQKVIPCLDFATGKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPESKSMGIQEDK 95
+QK++PC + P P CC N I A D CLC + KS+ + ++
Sbjct: 42 IQKLMPCQPYLHLATPPPAT-CCMPLNEIVAKDATCLCAVFNNVDM----LKSLNLTKEN 96
Query: 96 LLQLPTVCHVNGANISDCPSKSKTD 120
L LP C A++S C + + T+
Sbjct: 97 ALDLPKACGAK-ADVSLCKTSAGTN 120
>At1g73560 lipid transfer protein, putative
Length = 112
Score = 41.6 bits (96), Expect = 1e-04
Identities = 22/72 (30%), Positives = 32/72 (43%), Gaps = 6/72 (8%)
Query: 36 VQKVIPCLDFATGKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPE-SKSMGIQED 94
+QK++PC + P P CC I D CLC +PE K++ + ++
Sbjct: 34 LQKLLPCQPYIHSLNPPPPPSCCGPMKEIVEKDAPCLCIAF-----NNPEVLKALNLTKE 88
Query: 95 KLLQLPTVCHVN 106
L LP C VN
Sbjct: 89 NALLLPKACGVN 100
>At4g33355 non-specific lipid transfer protein
Length = 109
Score = 40.0 bits (92), Expect = 3e-04
Identities = 31/98 (31%), Positives = 44/98 (44%), Gaps = 5/98 (5%)
Query: 10 MCLCVLALIIGGCNGAEDLASKCGSVVQKVIPCLDFA-TGKAPTPKKECCDAANSIKATD 68
M L V+ +++G E +A C V + CL + G P+P CC+ NS+KA
Sbjct: 1 MLLLVITILLGIAYHGEAIA--CPQVNMYLAQCLPYLKAGGNPSPM--CCNGLNSLKAAA 56
Query: 69 PECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVN 106
PE + S + GI +D QLP C VN
Sbjct: 57 PEKADRQVACNCLKSVANTIPGINDDFAKQLPAKCGVN 94
>At5g64080 predicted GPI-anchored protein
Length = 173
Score = 39.7 bits (91), Expect = 4e-04
Identities = 20/84 (23%), Positives = 36/84 (42%), Gaps = 7/84 (8%)
Query: 32 CGSVVQKVIPCLDFAT--GKAPTPKKECCDAANSIKATDPECLCYIIQQTHKGSPESKSM 89
C +++ + CL F + G P+ CC ++ D +CLC + S S +
Sbjct: 43 CSTLILNMADCLSFVSSGGTVAKPEGTCCSGLKTVLKADSQCLCEAFK-----SSASLGV 97
Query: 90 GIQEDKLLQLPTVCHVNGANISDC 113
+ K LP C ++ +I+ C
Sbjct: 98 TLNITKASTLPAACKLHAPSIATC 121
>At2g48140 unknown protein
Length = 200
Score = 38.5 bits (88), Expect = 8e-04
Identities = 26/99 (26%), Positives = 44/99 (44%), Gaps = 10/99 (10%)
Query: 16 ALIIGGCNGAEDLASKC-GSVVQKVIPCLDFATGKAP---TPKKECCDAANSIKATDPEC 71
+ ++GG + +++ C S++ PCL+F TG + TP CCD+ ++ T C
Sbjct: 13 SFMLGG--QGQQISTPCTSSMISTFTPCLNFITGSSGGSVTPTAGCCDSLKTLTNTGMGC 70
Query: 72 LCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVNGANI 110
C I+ + + I L LP C + G I
Sbjct: 71 ACLIL----TANVPLPTGFINRTLALALPRACKMGGVPI 105
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.134 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,850,987
Number of Sequences: 26719
Number of extensions: 108665
Number of successful extensions: 295
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 248
Number of HSP's gapped (non-prelim): 52
length of query: 123
length of database: 11,318,596
effective HSP length: 87
effective length of query: 36
effective length of database: 8,994,043
effective search space: 323785548
effective search space used: 323785548
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC147538.3