BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147538.1 + phase: 0
(138 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g27300 Unknown protein (At1g27300; F17L21.9) 85 1e-17
At3g66652 hypothetical protein 32 0.080
At4g29160 unknown protein 30 0.31
At3g52570 unknown protein 30 0.40
At1g60190 29 0.68
At5g27450 mevalonate kinase 28 1.5
At3g25590 hypothetical protein 28 1.5
At5g46600 putative protein 27 2.6
At3g13150 hypothetical protein 27 2.6
At1g08125 DNA ligase 27 2.6
At3g14170 unknown protein 27 3.4
At2g26550 heme oxygenase 2 (HO2) 27 3.4
At5g25590 unknown protein (At5g25590) 27 4.4
At4g13050 oleoyl-[acyl-carrier-protein] hydrolase-like protein 27 4.4
At4g08880 hypothetical protein 27 4.4
At1g58290 putative protein 27 4.4
At5g51430 putative protein 26 5.8
At5g45790 tyrosine-specific protein phosphatase-like protein 26 5.8
At4g24190 HSP90-like protein 26 5.8
At4g20260 endomembrane-associated protein 26 5.8
>At1g27300 Unknown protein (At1g27300; F17L21.9)
Length = 200
Score = 84.7 bits (208), Expect = 1e-17
Identities = 55/141 (39%), Positives = 77/141 (54%), Gaps = 19/141 (13%)
Query: 3 KGGETVKQSEASSPLKQ-VDSAIEKTKGE---TADGDKDEIIKELKKVKKQNFVTHCLLS 58
KG E V E ++ DS+ K K E + +EI K++KKVKKQN +TH LLS
Sbjct: 54 KGKEDVITGEEEEKEEESADSSPSKGKSEGQRQLEESIEEIAKDIKKVKKQNKITHVLLS 113
Query: 59 AMIVLTVAWQLSEVSLVMRVKDGMNHPFRSVGNMFKEMVK-----------DVSGSNGQD 107
A I+LT+ WQLSE S++ +KD ++HP RS+G M M K S SN Q+
Sbjct: 114 ATIILTLVWQLSEYSMIFMLKDRISHPVRSIGGMLNGMFKGKLRPIKNQLAGTSNSNDQN 173
Query: 108 AAGDKENNESTSLPSLKIPDM 128
G N S + P L++P++
Sbjct: 174 NHG----NGSHTGPQLQVPEL 190
>At3g66652 hypothetical protein
Length = 980
Score = 32.3 bits (72), Expect = 0.080
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Query: 2 SKGGETVKQSEASSPLKQVDSAIEKTKGETADGDKD--EIIKELKKVKKQN 50
S GG+T++Q + P K + + EK DK+ EII+E+K V+ N
Sbjct: 875 SNGGDTIEQQDVQKPRKLMGKSEEKAMQNRDINDKEEGEIIEEVKGVEIDN 925
>At4g29160 unknown protein
Length = 219
Score = 30.4 bits (67), Expect = 0.31
Identities = 14/49 (28%), Positives = 26/49 (52%)
Query: 11 SEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVKKQNFVTHCLLSA 59
+E + +KQ+ A+ G AD D+DE+ EL +++ + + L A
Sbjct: 130 NEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPA 178
>At3g52570 unknown protein
Length = 335
Score = 30.0 bits (66), Expect = 0.40
Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 3/63 (4%)
Query: 12 EASSPLKQV---DSAIEKTKGETADGDKDEIIKELKKVKKQNFVTHCLLSAMIVLTVAWQ 68
E++SP KQ+ DS + K E +DG+ ++++K L+ + L+ M+ L +
Sbjct: 159 ESASPPKQLWVLDSVPGEVKAEKSDGEVEKVLKTLQSLPSSIPSRKWLVDRMVELGFSRS 218
Query: 69 LSE 71
LSE
Sbjct: 219 LSE 221
>At1g60190
Length = 686
Score = 29.3 bits (64), Expect = 0.68
Identities = 28/115 (24%), Positives = 48/115 (41%), Gaps = 16/115 (13%)
Query: 3 KGGETVKQSEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKV----KKQNFVTHCLLS 58
KG + V +E+ + + E GE GD++E++K L ++ K F CL+
Sbjct: 355 KGKKKVDVAESLAAEEAGKLTAEFLAGELIKGDEEEMVKALVEIRILTKTSTFYRSCLVE 414
Query: 59 AMIVLTVAWQLSEVSLVMRVKDGMNHPFRSVGNMFKEMVKDVSGSN---GQDAAG 110
A +V + ++R D G M + KD++G G+D G
Sbjct: 415 AGVV-------ESLMKILRSDDPRIQENAMAGIM--NLSKDIAGKTRIVGEDGGG 460
>At5g27450 mevalonate kinase
Length = 378
Score = 28.1 bits (61), Expect = 1.5
Identities = 18/52 (34%), Positives = 32/52 (60%), Gaps = 4/52 (7%)
Query: 22 SAIEKTKGETADGDKDEIIKELKKVKK----QNFVTHCLLSAMIVLTVAWQL 69
+AI ++K ET+ +K+E IKEL ++ + V+H + A+I+ TV +L
Sbjct: 276 AAIIQSKDETSVTEKEERIKELMEMNQGLLLSMGVSHSSIEAVILTTVKHKL 327
>At3g25590 hypothetical protein
Length = 414
Score = 28.1 bits (61), Expect = 1.5
Identities = 27/110 (24%), Positives = 44/110 (39%), Gaps = 10/110 (9%)
Query: 15 SPLKQVDSAIEKTKGETADGDKDEIIKELKKVKKQNFVTHCLLSAMIVLTVAWQLSEVSL 74
SP + VD+A++ GE I +E K +K+ V ++ S S
Sbjct: 63 SPTRTVDAAVDAAVGENVVRISSLIDEERAKQRKET------KQRKTEEVVVFKRSSSSC 116
Query: 75 VMRVKDGMNHPFRSVGNMFKEM----VKDVSGSNGQDAAGDKENNESTSL 120
V K NH F +G F+++ +D +N D+ NN+ L
Sbjct: 117 VEINKRTKNHRFSRIGRFFRKINLKKERDFEKNNNNDSWVLDYNNDVKKL 166
>At5g46600 putative protein
Length = 539
Score = 27.3 bits (59), Expect = 2.6
Identities = 14/46 (30%), Positives = 25/46 (53%), Gaps = 4/46 (8%)
Query: 5 GETVKQSEASSPLKQVD----SAIEKTKGETADGDKDEIIKELKKV 46
GET+++++ SPL S++ K A G+K + K+L K+
Sbjct: 434 GETIEENDTVSPLPLNSVVSLSSLRSVKKSAATGEKRRLRKQLSKI 479
>At3g13150 hypothetical protein
Length = 551
Score = 27.3 bits (59), Expect = 2.6
Identities = 24/105 (22%), Positives = 48/105 (44%), Gaps = 16/105 (15%)
Query: 33 DGDKDEIIKELKKVKKQNF----VTHCLLSAMIV----LTVAWQLSEVSLVMRVKDGMNH 84
D + +E++K ++K++ VT+C+L ++ L A ++SE + + H
Sbjct: 311 DNNLEEVMKCYNEMKEKGLTPDTVTYCMLIPLLCKKGDLDRAVEVSE--------EAIKH 362
Query: 85 PFRSVGNMFKEMVKDVSGSNGQDAAGDKENNESTSLPSLKIPDMT 129
S NM+K +V+ + G+ D A N +PD++
Sbjct: 363 KLLSRPNMYKPVVERLMGAGKIDEATQLVKNGKLQSYFRYLPDLS 407
>At1g08125 DNA ligase
Length = 315
Score = 27.3 bits (59), Expect = 2.6
Identities = 21/83 (25%), Positives = 43/83 (51%), Gaps = 7/83 (8%)
Query: 25 EKTKGETADG-DKDEIIKELKKVKKQNFVT----HCLLSAMIVLTVAWQLSEVSLVMRVK 79
+K+ E++ +DE++ + + K + V H + V+T +L+E SL+MR++
Sbjct: 225 QKSAAESSGNVSRDEVVIDTDETKCETKVPTGECHKRVEEEAVVTHGPRLNEDSLLMRLR 284
Query: 80 DG--MNHPFRSVGNMFKEMVKDV 100
DG R G + ++++DV
Sbjct: 285 DGKLSEWEMRRYGTVAAQLLRDV 307
>At3g14170 unknown protein
Length = 505
Score = 26.9 bits (58), Expect = 3.4
Identities = 14/47 (29%), Positives = 28/47 (58%), Gaps = 3/47 (6%)
Query: 11 SEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVKKQNFVTHCLL 57
+E S LKQ + +EK K + D + +++ ++++KK+ + CLL
Sbjct: 450 AETLSQLKQANEWLEKVKNDNLSSD-NSLLENIERLKKK--IYSCLL 493
>At2g26550 heme oxygenase 2 (HO2)
Length = 299
Score = 26.9 bits (58), Expect = 3.4
Identities = 13/47 (27%), Positives = 26/47 (54%)
Query: 19 QVDSAIEKTKGETADGDKDEIIKELKKVKKQNFVTHCLLSAMIVLTV 65
+ D+ E+ + E D D DE+ +E K K+ F+ + + S ++ T+
Sbjct: 101 KTDTEKEEEEEEEDDDDDDEVKEETWKPSKEGFLKYLVDSKLVFDTI 147
>At5g25590 unknown protein (At5g25590)
Length = 775
Score = 26.6 bits (57), Expect = 4.4
Identities = 14/49 (28%), Positives = 28/49 (56%), Gaps = 2/49 (4%)
Query: 2 SKGGETVKQSEASSPLK--QVDSAIEKTKGETADGDKDEIIKELKKVKK 48
S G+ V++ E +P K + + E+ E + +++E++ E+KK KK
Sbjct: 251 SGSGKVVEEMEPKTPEKVEEEEEEDEEEDEEEEEEEEEEVVVEVKKKKK 299
>At4g13050 oleoyl-[acyl-carrier-protein] hydrolase-like protein
Length = 367
Score = 26.6 bits (57), Expect = 4.4
Identities = 11/33 (33%), Positives = 21/33 (63%)
Query: 95 EMVKDVSGSNGQDAAGDKENNESTSLPSLKIPD 127
E+V ++G+NG + +E+NES L L++ +
Sbjct: 317 EVVSKLTGTNGSTTSSKREHNESHFLHILRLSE 349
>At4g08880 hypothetical protein
Length = 1175
Score = 26.6 bits (57), Expect = 4.4
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Query: 73 SLVMRVKDGMNHPFRSVGNMFKEMVKDVSGSN-----GQDAAGDKENNESTSLPSLKIPD 127
S + RV ++ P SVG KE KDV G + G+ + N ++ + ++PD
Sbjct: 409 SRLTRVSHDVDTPALSVGCNSKEEDKDVPGVHTTAVGGRSKPSKPKKNVFFAVDTTELPD 468
Query: 128 MTNVDVPNF 136
D+ F
Sbjct: 469 SNKEDMQGF 477
>At1g58290 putative protein
Length = 543
Score = 26.6 bits (57), Expect = 4.4
Identities = 18/74 (24%), Positives = 35/74 (46%), Gaps = 3/74 (4%)
Query: 61 IVLTVAWQLSEVSLVMRVKDGMNHPFRSVGNMFKEMV---KDVSGSNGQDAAGDKENNES 117
+VL L++V V++V G+N R++ +FK + K V + ++ +
Sbjct: 204 LVLGEGQILAQVKQVVKVGQGVNGFGRNISGLFKHAITVGKRVRTETNIASGAVSVSSAA 263
Query: 118 TSLPSLKIPDMTNV 131
L +K+P +NV
Sbjct: 264 VELALMKLPQSSNV 277
>At5g51430 putative protein
Length = 836
Score = 26.2 bits (56), Expect = 5.8
Identities = 16/46 (34%), Positives = 25/46 (53%), Gaps = 2/46 (4%)
Query: 6 ETVKQSEASSPLKQV--DSAIEKTKGETADGDKDEIIKELKKVKKQ 49
ETV++ E S P V ++A+E+ G T + DE+I L + Q
Sbjct: 443 ETVRRMEESIPQVVVLLEAAVERCIGFTGGSEADELILALDDIMLQ 488
>At5g45790 tyrosine-specific protein phosphatase-like protein
Length = 423
Score = 26.2 bits (56), Expect = 5.8
Identities = 12/30 (40%), Positives = 19/30 (63%)
Query: 17 LKQVDSAIEKTKGETADGDKDEIIKELKKV 46
LK VDSA + + T D D+II +++K+
Sbjct: 39 LKPVDSAQTRLENRTRDHQLDKIIPQIRKL 68
>At4g24190 HSP90-like protein
Length = 823
Score = 26.2 bits (56), Expect = 5.8
Identities = 13/41 (31%), Positives = 25/41 (60%)
Query: 9 KQSEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVKKQ 49
K+ E P+++ +SA E+T+ + + +K+E +E KKQ
Sbjct: 286 KEVETEVPVEEDESADEETETTSTEEEKEEDAEEEDGEKKQ 326
>At4g20260 endomembrane-associated protein
Length = 225
Score = 26.2 bits (56), Expect = 5.8
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Query: 8 VKQSEASSPLKQVDSAIEKTKGETADGDKDEIIKELKK 45
V++ +A P K + A K E G+K+EI++E KK
Sbjct: 141 VEEVKAEEPAKTEEPA----KTEGTSGEKEEIVEETKK 174
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.308 0.127 0.343
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,114,705
Number of Sequences: 26719
Number of extensions: 120798
Number of successful extensions: 439
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 24
Number of HSP's that attempted gapping in prelim test: 426
Number of HSP's gapped (non-prelim): 37
length of query: 138
length of database: 11,318,596
effective HSP length: 89
effective length of query: 49
effective length of database: 8,940,605
effective search space: 438089645
effective search space used: 438089645
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC147538.1