BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147537.12 + phase: 0
(125 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g59320 nonspecific lipid-transfer protein precursor - like 102 5e-23
At2g38540 putative nonspecific lipid-transfer protein 95 1e-20
At5g59310 nonspecific lipid-transfer protein precursor - like 92 5e-20
At2g15050 putative lipid transfer protein 91 1e-19
At3g08770 putative nonspecific lipid-transfer protein 90 3e-19
At2g38530 putative nonspecific lipid-transfer protein 89 7e-19
At5g01870 lipid-transfer protein-like 88 1e-18
At3g51590 lipid transfer protein-like protein 85 8e-18
At4g33355 non-specific lipid transfer protein 79 6e-16
At3g51600 non-specific lipid transfer protein 77 3e-15
At2g18370 putative lipid transfer protein 76 4e-15
At2g48130 predicted GPI-anchored protein 48 1e-06
At2g15325 putative protein 45 9e-06
At5g64080 predicted GPI-anchored protein 42 6e-05
At2g44290 non-specific lipid transfer protein (nLTP) like protein 42 1e-04
At1g73890 predicted GPI-anchored protein 41 2e-04
At3g22600 predicted GPI-anchored protein 40 3e-04
At2g44300 predicted GPI-anchored protein 40 3e-04
At5g13900 predicted GPI-anchored protein 39 7e-04
At4g28395 ATA7 39 7e-04
>At5g59320 nonspecific lipid-transfer protein precursor - like
Length = 115
Score = 102 bits (254), Expect = 5e-23
Identities = 49/107 (45%), Positives = 65/107 (59%), Gaps = 4/107 (3%)
Query: 11 TCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVT 70
TCL + +C+V + A+SC V L PC Y++ GG VP CC GVK +N+ A T
Sbjct: 9 TCLVLTVCIVASVDA---AISCGTVAGSLAPCATYLS-KGGLVPPSCCAGVKTLNSMAKT 64
Query: 71 KSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
DRQ ACRCI++ +I GLN + +GLP KCGV +PY + ST C
Sbjct: 65 TPDRQQACRCIQSTAKSISGLNPSLASGLPGKCGVSIPYPISMSTNC 111
>At2g38540 putative nonspecific lipid-transfer protein
Length = 118
Score = 94.7 bits (234), Expect = 1e-20
Identities = 50/114 (43%), Positives = 70/114 (60%), Gaps = 4/114 (3%)
Query: 7 LVKVTCLTMMMCMVLGLPQTLDA-LSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAIN 65
++K+ CL ++ CM++ P T +A LSC V + L C+ YV GG +P CC GVK +N
Sbjct: 4 VMKLACL-LLACMIVAGPITSNAALSCGSVNSNLAACIGYVL-QGGVIPPACCSGVKNLN 61
Query: 66 NQAVTKSDRQAACRCIKTATSAI-HGLNMDILAGLPSKCGVHLPYTLGPSTTCE 118
+ A T DRQ AC CI+ A A+ GLN AG+P CGV++PY + ST C+
Sbjct: 62 SIAKTTPDRQQACNCIQGAARALGSGLNAGRAAGIPKACGVNIPYKISTSTNCK 115
>At5g59310 nonspecific lipid-transfer protein precursor - like
Length = 112
Score = 92.4 bits (228), Expect = 5e-20
Identities = 46/107 (42%), Positives = 64/107 (58%), Gaps = 7/107 (6%)
Query: 11 TCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVT 70
TC + + +V + A++C V + L PC+ Y++ GG VP PCC GVK +N A T
Sbjct: 9 TCFVLTVFIVASVDA---AITCGTVASSLSPCLGYLS-KGGVVPPPCCAGVKKLNGMAQT 64
Query: 71 KSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
DRQ ACRC++ SA G+N + +GLP KCGV +PY + ST C
Sbjct: 65 TPDRQQACRCLQ---SAAKGVNPSLASGLPGKCGVSIPYPISTSTNC 108
>At2g15050 putative lipid transfer protein
Length = 123
Score = 90.9 bits (224), Expect = 1e-19
Identities = 47/119 (39%), Positives = 68/119 (56%), Gaps = 4/119 (3%)
Query: 7 LVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQP-CCDGVKAIN 65
L+K+ CL + + G ALSC +V + L PC Y+T G P P CC+GV+ +N
Sbjct: 4 LMKLGCLVFVFVIAAGPITAKAALSCGEVNSNLKPCTGYLTNGGITSPGPQCCNGVRKLN 63
Query: 66 NQAVTKSDRQAACRCIKTATSAI-HGLNMDILAGLPSKCGVHLPYT--LGPSTTCEKYV 121
+T DR+ ACRCIK A + GLN D AG+P +CG+ +PY+ + +T C Y+
Sbjct: 64 GMVLTTLDRRQACRCIKNAARNVGPGLNADRAAGIPRRCGIKIPYSTQIRFNTKCNTYI 122
>At3g08770 putative nonspecific lipid-transfer protein
Length = 113
Score = 89.7 bits (221), Expect = 3e-19
Identities = 52/111 (46%), Positives = 63/111 (55%), Gaps = 9/111 (8%)
Query: 13 LTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKS 72
L + +C+VL L A+SC V L PC+ YVT GG VP CC+G+ + +QA T
Sbjct: 4 LLLAVCLVLALHCGEAAVSCNTVIADLYPCLSYVT-QGGPVPTLCCNGLTTLKSQAQTSV 62
Query: 73 DRQAACRCIKTATSAIHGLNM-----DILAGLPSKCGVHLPYTLGPSTTCE 118
DRQ CRCIK SAI GL + LPSKCGV LPY PST C+
Sbjct: 63 DRQGVCRCIK---SAIGGLTLSPRTIQNALELPSKCGVDLPYKFSPSTDCD 110
>At2g38530 putative nonspecific lipid-transfer protein
Length = 118
Score = 88.6 bits (218), Expect = 7e-19
Identities = 48/113 (42%), Positives = 69/113 (60%), Gaps = 4/113 (3%)
Query: 7 LVKVTCLTMMMCMVLGLPQTLDAL-SCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAIN 65
++K+ C+ ++ CM++ P T +AL SC V L C+ Y+T G + Q CC+GV +
Sbjct: 4 VMKLACM-VLACMIVAGPITANALMSCGTVNGNLAGCIAYLT-RGAPLTQGCCNGVTNLK 61
Query: 66 NQAVTKSDRQAACRCIKTATSAIH-GLNMDILAGLPSKCGVHLPYTLGPSTTC 117
N A T DRQ ACRC+++A A+ GLN AGLPS C V++PY + ST C
Sbjct: 62 NMASTTPDRQQACRCLQSAAKAVGPGLNTARAAGLPSACKVNIPYKISASTNC 114
>At5g01870 lipid-transfer protein-like
Length = 116
Score = 87.8 bits (216), Expect = 1e-18
Identities = 40/91 (43%), Positives = 55/91 (59%), Gaps = 3/91 (3%)
Query: 29 ALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAI 88
A+SC V+ L PCV YV GG +P CC+G++ ++ QA + SD+Q CRCIK+ +
Sbjct: 23 AISCNAVQANLYPCVVYVV-QGGAIPYSCCNGIRMLSKQATSASDKQGVCRCIKSVVGRV 81
Query: 89 --HGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
+ + A LP KCGV LPY + PST C
Sbjct: 82 SYSSIYLKKAAALPGKCGVKLPYKIDPSTNC 112
>At3g51590 lipid transfer protein-like protein
Length = 119
Score = 85.1 bits (209), Expect = 8e-18
Identities = 43/109 (39%), Positives = 64/109 (58%), Gaps = 3/109 (2%)
Query: 10 VTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAV 69
+TCL +++ + + P T + C V + L C+ Y+T N G +P CC GVK++ A
Sbjct: 8 ITCL-IVLTIYMASP-TESTIQCGTVTSTLAQCLTYLT-NSGPLPSQCCVGVKSLYQLAQ 64
Query: 70 TKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTCE 118
T DR+ C C+K A I GLN D++A LP+ CGV +PY + ST C+
Sbjct: 65 TTPDRKQVCECLKLAGKEIKGLNTDLVAALPTTCGVSIPYPISFSTNCD 113
>At4g33355 non-specific lipid transfer protein
Length = 109
Score = 79.0 bits (193), Expect = 6e-16
Identities = 36/105 (34%), Positives = 60/105 (56%), Gaps = 1/105 (0%)
Query: 13 LTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKS 72
L +++ ++LG+ +A++C QV L C+PY+ G P CC+G+ ++ A K+
Sbjct: 2 LLLVITILLGIAYHGEAIACPQVNMYLAQCLPYLKAGGNPSPM-CCNGLNSLKAAAPEKA 60
Query: 73 DRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
DRQ AC C+K+ + I G+N D LP+KCGV++ + C
Sbjct: 61 DRQVACNCLKSVANTIPGINDDFAKQLPAKCGVNIGVPFSKTVDC 105
>At3g51600 non-specific lipid transfer protein
Length = 118
Score = 76.6 bits (187), Expect = 3e-15
Identities = 44/113 (38%), Positives = 65/113 (56%), Gaps = 4/113 (3%)
Query: 7 LVKVTCLTMMMCMVLGLPQTLDA-LSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAIN 65
L+K++ L +++CM++ P +A +SC V L C Y+T GG++P+ CC GV+ +N
Sbjct: 4 LLKLSTL-VIVCMLVTAPMASEAAISCGAVTGSLGQCYNYLT-RGGFIPRGCCSGVQRLN 61
Query: 66 NQAVTKSDRQAACRCIKTATSAIHG-LNMDILAGLPSKCGVHLPYTLGPSTTC 117
+ A T DRQ ACRCI+ A A+ LN A LP C V + Y + T C
Sbjct: 62 SLARTTRDRQQACRCIQGAARALGSRLNAGRAARLPGACRVRISYPISARTNC 114
>At2g18370 putative lipid transfer protein
Length = 116
Score = 76.3 bits (186), Expect = 4e-15
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 1/89 (1%)
Query: 29 ALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAI 88
A+SC V L PCV Y+T G P+ CCDGVK++ T +D++AAC+CIK+ +++
Sbjct: 25 AISCSVVLQDLQPCVSYLTSGSGNPPETCCDGVKSLAAATTTSADKKAACQCIKSVANSV 84
Query: 89 HGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
+ ++ L S CG LP P+ C
Sbjct: 85 -TVKPELAQALASNCGASLPVDASPTVDC 112
>At2g48130 predicted GPI-anchored protein
Length = 183
Score = 48.1 bits (113), Expect = 1e-06
Identities = 30/89 (33%), Positives = 41/89 (45%), Gaps = 12/89 (13%)
Query: 31 SCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSAIHG 90
SC+ T L PC+ Y+TGN QPCC + +V KS Q C + + I G
Sbjct: 29 SCVSTLTTLSPCLSYITGNSTTPSQPCCSRL-----DSVIKSSPQCICSAVNSPIPNI-G 82
Query: 91 LNMDILAG--LPSKCGVHLPYTLGPSTTC 117
LN++ LP+ C + P P T C
Sbjct: 83 LNINRTQALQLPNACNIQTP----PLTQC 107
>At2g15325 putative protein
Length = 118
Score = 45.1 bits (105), Expect = 9e-06
Identities = 32/118 (27%), Positives = 48/118 (40%), Gaps = 3/118 (2%)
Query: 1 MSSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQP-CCD 59
M S+ + V +T+ M L + C + L PC+ Y+ P P CC
Sbjct: 1 MRKSISIAFVIAITIFMSH-LNVFTVYSLTPCEEATNLLTPCLRYLWAPPEAKPSPECCS 59
Query: 60 GVKAINNQAVTKSDRQAACRCIKTATSAIHGLNMDILAGLPSKCGVHLPYTLGPSTTC 117
G+ +N T DR C C+ ++ +AI + LP C V L +GP C
Sbjct: 60 GLDKVNKGVKTYDDRHDMCICL-SSEAAITSADQYKFDNLPKLCNVALFAPVGPKFDC 116
>At5g64080 predicted GPI-anchored protein
Length = 173
Score = 42.4 bits (98), Expect = 6e-05
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 9/83 (10%)
Query: 29 ALSCLQVETKLMPCVPYVTGNGGYVPQP---CCDGVKAINNQAVTKSDRQAACRCIKTAT 85
++ C + + C+ +V+ +GG V +P CC G+K V K+D Q C K++
Sbjct: 40 SVDCSTLILNMADCLSFVS-SGGTVAKPEGTCCSGLKT-----VLKADSQCLCEAFKSSA 93
Query: 86 SAIHGLNMDILAGLPSKCGVHLP 108
S LN+ + LP+ C +H P
Sbjct: 94 SLGVTLNITKASTLPAACKLHAP 116
>At2g44290 non-specific lipid transfer protein (nLTP) like protein
Length = 205
Score = 41.6 bits (96), Expect = 1e-04
Identities = 31/120 (25%), Positives = 47/120 (38%), Gaps = 11/120 (9%)
Query: 1 MSSSMVLVKVTCLTMMMCMVL----GLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQP 56
M S + V T + ++M ++ G + D C + C+PYV G
Sbjct: 1 MESRKIKVMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPD 60
Query: 57 CCDGVKAINNQAVTKSDRQAACRCIKTATSAIHGL--NMDILAGLPSKCGVHLPYTLGPS 114
CC G+K + N SD + C I+ GL N+ + LPS C T P+
Sbjct: 61 CCSGLKQVIN-----SDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPA 115
>At1g73890 predicted GPI-anchored protein
Length = 193
Score = 40.8 bits (94), Expect = 2e-04
Identities = 31/109 (28%), Positives = 46/109 (41%), Gaps = 13/109 (11%)
Query: 1 MSSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDG 60
M+SS +L+ T L + L + +C L PC P+V G QPCCD
Sbjct: 1 MASSTLLI--TLLISLSAFFLRMVLAQVPATCASRLLSLAPCGPFVQGFAQLPAQPCCDS 58
Query: 61 VKAINNQAVTKSDRQAACRCI---KTAT-SAIHGLNMDILAGLPSKCGV 105
+ I +Q +A C C+ T+T S +N + LP C +
Sbjct: 59 LNQIYSQ-------EATCLCLFLNNTSTLSPAFPINQTLALQLPPLCNI 100
>At3g22600 predicted GPI-anchored protein
Length = 170
Score = 40.0 bits (92), Expect = 3e-04
Identities = 29/109 (26%), Positives = 43/109 (38%), Gaps = 10/109 (9%)
Query: 1 MSSSMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDG 60
M M LV +T +M + Q+ SC + PC+ Y+TGN Q CC+
Sbjct: 1 MKMEMGLVFLTVFMAVMSSTMVSAQS----SCTNALISMSPCLNYITGNSTSPNQQCCNQ 56
Query: 61 VKAINNQAVTKSDRQAACRCIKTATSAIH-GLNMDILAGLPSKCGVHLP 108
+ V +S C+ + S + +N GLP C V P
Sbjct: 57 L-----SRVVQSSPDCLCQVLNGGGSQLGINVNQTQALGLPRACNVQTP 100
>At2g44300 predicted GPI-anchored protein
Length = 204
Score = 40.0 bits (92), Expect = 3e-04
Identities = 26/113 (23%), Positives = 46/113 (40%), Gaps = 7/113 (6%)
Query: 4 SMVLVKVTCLTMMMCMVLGLPQTLDALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKA 63
+++ + + + M + +T D C + + C+PYV G CC G+K
Sbjct: 7 NLMATAIALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQ 66
Query: 64 INNQAVTKSDRQAACRCIKTATSAIHGL--NMDILAGLPSKCGVHLPYTLGPS 114
+ N S+++ C I+ GL N+ + LPS C T P+
Sbjct: 67 VLN-----SNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPA 114
>At5g13900 predicted GPI-anchored protein
Length = 151
Score = 38.9 bits (89), Expect = 7e-04
Identities = 25/82 (30%), Positives = 45/82 (54%), Gaps = 12/82 (14%)
Query: 29 ALSCLQVETKLMPCVPYVTGNGGYVPQPCCDGVKAINNQAVTKSDRQAACRCIKTATSA- 87
++SCL +L PC+ Y+ G VPQ CC+ +K +V +++ + CR I S+
Sbjct: 29 SVSCLN---QLAPCLNYLNGTKE-VPQVCCNPLK-----SVIRNNPECLCRMISNRWSSQ 79
Query: 88 --IHGLNMDILAGLPSKCGVHL 107
G++++ LP++CG H+
Sbjct: 80 AERAGIDVNDAQMLPARCGEHV 101
>At4g28395 ATA7
Length = 180
Score = 38.9 bits (89), Expect = 7e-04
Identities = 30/130 (23%), Positives = 51/130 (39%), Gaps = 12/130 (9%)
Query: 3 SSMVLVKVTCLTMMMCMVLGLPQTLDALS-CLQVETKLMPCVPYVTGNGGYVPQPCCDGV 61
SSM + + + ++ M + Q + L C V MPC+ +V G CC GV
Sbjct: 39 SSMKIHAILVVAFLVLMKTAVSQDNNPLEHCRDVFVSFMPCMGFVEGIFQQPSPDCCRGV 98
Query: 62 KAINNQAVTKS----------DRQAACRCIKTATSAIH-GLNMDILAGLPSKCGVHLPYT 110
+NN S + + C CI+ +A H + LP +C + L +
Sbjct: 99 THLNNVVKFTSPGSRNRQDSGETERVCLCIEIMGNANHLPFLPAAINNLPLRCSLTLSFP 158
Query: 111 LGPSTTCEKY 120
+ C ++
Sbjct: 159 ISVDMDCSQF 168
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.135 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,741,296
Number of Sequences: 26719
Number of extensions: 96586
Number of successful extensions: 221
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 169
Number of HSP's gapped (non-prelim): 45
length of query: 125
length of database: 11,318,596
effective HSP length: 87
effective length of query: 38
effective length of database: 8,994,043
effective search space: 341773634
effective search space used: 341773634
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 54 (25.4 bits)
Medicago: description of AC147537.12