BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147536.6 + phase: 0
(281 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g59350 unknown protein 152 2e-37
At2g39560 unknown protein 135 2e-32
At3g52480 unknown protein 47 1e-05
At2g31590 unknown protein 33 0.22
At3g11640 unknown protein 32 0.38
At3g10900 putative (1-4)-beta-mannan endohydrolase 32 0.50
At5g11990 predicted GPI-anchored protein 30 1.5
At2g28480 hypothetical protein 30 1.9
At2g13370 pseudogene 29 2.5
At5g50780 putative protein 28 7.2
At4g11460 serine/threonine kinase-like protein 28 7.2
At4g39790 putative protein 27 9.4
At3g62140 hypothetical protein 27 9.4
At3g58670 unknown protein 27 9.4
>At5g59350 unknown protein
Length = 287
Score = 152 bits (384), Expect = 2e-37
Identities = 106/249 (42%), Positives = 146/249 (58%), Gaps = 33/249 (13%)
Query: 1 MKSLSSVGLGLSIVFGCLLLALVAEVYYLL---WWKKKIIKREI----ENDYGNGNPLKE 53
MK++S +G+GLS++FG LLLALV EVYYLL KK++I +E E + KE
Sbjct: 1 MKTISGLGIGLSLMFGFLLLALVGEVYYLLRCKKHKKRVISQESEEEKEEEQQQNGYAKE 60
Query: 54 LFYMFCWKRPSTSLRQTGFTPPELCSSMRINDNFVHDPQVQTSKEFLFKPYGEDVIDADY 113
L +FC+K+P + + G E + +N++ D ++ K G+ +A+
Sbjct: 61 LIQLFCFKKPQSLQQNNGGREGE----VSMNEDGNPDLELGLMKHL---NGGDLGFEAEL 113
Query: 114 MMQHHHDGLLGHPRFLFTIVEESKEDLESEDG---KCGKDSRGRSLGDL-LDVETPYLTP 169
M H+ RFLFTI+EE+K DLESEDG + G SR RSL D+ D TP TP
Sbjct: 114 MKLHNQ-------RFLFTIMEETKADLESEDGGKSRLGSRSRRRSLSDVPNDCNTPGFTP 166
Query: 170 IASPHFFTPMNCSPYCSPYNQHGFNPLFESTTDAEFN---RLKSSPPPKFKFLQEAEEKL 226
+ASP + SP S Y HGFNPLFES + EFN R SSPPPKFKF+++AEEKL
Sbjct: 167 LASP----KKSSSPLES-YPHHGFNPLFESDGELEFNKFFRSSSSPPPKFKFMRDAEEKL 221
Query: 227 RRKMQDDNK 235
R+++ ++ K
Sbjct: 222 RKRLIEEAK 230
>At2g39560 unknown protein
Length = 233
Score = 135 bits (341), Expect = 2e-32
Identities = 106/251 (42%), Positives = 139/251 (55%), Gaps = 48/251 (19%)
Query: 1 MKSLSSVGLGLSIVFGCLLLALVAEVYYLLWWKKKIIKR--EIENDYGNGNPLKELFYMF 58
M+SLSSVGL LSIVFGCLLLAL+AE+YYLLW KK+ R + NDY +EL ++F
Sbjct: 1 MRSLSSVGLALSIVFGCLLLALLAELYYLLWCKKRSTTRRPDFRNDYSTPG-TRELLFIF 59
Query: 59 CWKRPSTSLRQTGFTPPELCSSMRINDNFVHDPQVQTSKEFLFKPYGEDVIDADYMMQHH 118
C S+S + +P SS ++ D Q Q F+ G
Sbjct: 60 CC---SSSTNPSSSSP----SSSSFSNPKPIDTQQQCPLNNGFENVG------------- 99
Query: 119 HDGLLGHPRFLFTIVEESKEDLESEDGKCGKDSRGRSLGDL-LDVET-----PYLTPIAS 172
GL+ PRFLFTI+EE+ E++ESED ++G+SL DL L++E+ PYLTP AS
Sbjct: 100 GPGLV--PRFLFTIMEETVEEMESED---VVSTKGKSLNDLFLNMESGVITPPYLTPRAS 154
Query: 173 PHFFTPMNCSPYCSPYN--QHGFNPLFESTTDAEFNRL------------KSSPPPKFKF 218
P FTP N + + LFES++DAEFNRL SSP +FKF
Sbjct: 155 PSLFTPPLTPLLMESCNGRKEEISSLFESSSDAEFNRLVRSSPLSSSHSPSSSPLSRFKF 214
Query: 219 LQEAEEKLRRK 229
L++AEEKL +K
Sbjct: 215 LRDAEEKLYKK 225
>At3g52480 unknown protein
Length = 209
Score = 46.6 bits (109), Expect = 1e-05
Identities = 54/193 (27%), Positives = 78/193 (39%), Gaps = 38/193 (19%)
Query: 11 LSIVFGCLLLALVAEVYYLLWWKKKIIKREI---ENDYGNGN---------PLKELFYMF 58
L VF L+A+ A+ Y+LWWK++ +R I E D + P KEL Y F
Sbjct: 10 LVAVFAFCLVAVTAQFAYVLWWKRRFRRRSIAGSERDAFSSRGGDLTATPPPSKELLYFF 69
Query: 59 CWKRPSTSLRQTGFTPP----------ELCSSMRIN-DNFVHDPQVQTSKEFLFKPYGED 107
+ + R T P ++ S IN +N + P E LF
Sbjct: 70 LFCLENKQFRIGSATAPPLPAAAPPVNDVASKWSINGENLLCGP-----SETLF------ 118
Query: 108 VIDADYMMQ-HHHDGLLGHPRFLFTIVEESKEDLESEDGKCGKDSRGRSLG-DLLDVETP 165
I DY + H G + +FT E+ +D E E+ D + + TP
Sbjct: 119 TIAEDYTSESDHRTGEIDPRGSIFT--EDHVKDDEVEEEVVATDISDDEVDFSHYNQTTP 176
Query: 166 YLTPIASPHFFTP 178
+ TP ASP F+TP
Sbjct: 177 FSTPCASPPFYTP 189
>At2g31590 unknown protein
Length = 240
Score = 32.7 bits (73), Expect = 0.22
Identities = 24/86 (27%), Positives = 38/86 (43%), Gaps = 4/86 (4%)
Query: 182 SPYCSPYNQHGFNPLFESTTDAEFNRLKSS---PPPKFKFLQEAEEKLRR-KMQDDNKGI 237
+PY S F P TT + RL+SS P P + + E E RR + +D N +
Sbjct: 41 TPYFSSSRTAAFQPYTHPTTQSGIGRLQSSVAAPTPGVRRVYETGESSRRVEDRDSNNNL 100
Query: 238 NGNEVDNSLITIIVDKKYEREVNHHQ 263
+ + II+ ++ + E N Q
Sbjct: 101 HLGDSSKRKNMIILQREKDSEQNKQQ 126
>At3g11640 unknown protein
Length = 186
Score = 32.0 bits (71), Expect = 0.38
Identities = 25/61 (40%), Positives = 29/61 (46%), Gaps = 12/61 (19%)
Query: 127 RFLFTIVEESKEDLESEDGKCGKDSRGRSLGDLLDVE---------TPYLTPIASPHFFT 177
R LFTI EE DLE ED S G + V+ TP+LTP SP +FT
Sbjct: 97 RLLFTIEEE---DLELEDDDDDVISAEVDNGFEVQVDIPVEYSGDPTPFLTPCNSPPYFT 153
Query: 178 P 178
P
Sbjct: 154 P 154
>At3g10900 putative (1-4)-beta-mannan endohydrolase
Length = 408
Score = 31.6 bits (70), Expect = 0.50
Identities = 16/62 (25%), Positives = 31/62 (49%), Gaps = 2/62 (3%)
Query: 146 KCGKDSRGRSLGDLLDVETPYLTPIASPHFFTPMNCSPY--CSPYNQHGFNPLFESTTDA 203
+CG D G++L D ++ P++ + H + + Y SP ++ NP+ +T A
Sbjct: 204 RCGVDKSGKTLMDWINEMAPFVKSVDPNHLLSTGHEGFYGDSSPERKNSLNPVSANTVGA 263
Query: 204 EF 205
+F
Sbjct: 264 DF 265
>At5g11990 predicted GPI-anchored protein
Length = 181
Score = 30.0 bits (66), Expect = 1.5
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Query: 164 TPYLTPIASPHFFTPMNCSPYCSPYNQHGFNPLFESTTDAEFNRLKSSPPPKFKFLQ 220
+P ++P SP P++ SP P ++H PL +S + + PPP+F + +
Sbjct: 50 SPSMSPPPSPSL--PLSSSPPPPPPHKHSPPPLSQSLSPPPLITVIHPPPPRFYYFE 104
>At2g28480 hypothetical protein
Length = 372
Score = 29.6 bits (65), Expect = 1.9
Identities = 21/74 (28%), Positives = 35/74 (46%), Gaps = 4/74 (5%)
Query: 183 PYCSPYNQHGFNP--LFESTTDAEFNRLKSSPPPKFKFLQEAEEKLRRKMQDDNKGINGN 240
P C ++ HG+ L S +F + PP K+K+L + KL+RK + + + N
Sbjct: 48 PICRNFS-HGYGTVNLVISEGKPKFETRELDPPKKYKWLTKKRLKLKRKKEREERNA-AN 105
Query: 241 EVDNSLITIIVDKK 254
D +T+ KK
Sbjct: 106 RKDPRRLTVKGKKK 119
>At2g13370 pseudogene
Length = 1738
Score = 29.3 bits (64), Expect = 2.5
Identities = 19/57 (33%), Positives = 28/57 (48%), Gaps = 12/57 (21%)
Query: 208 LKSSPPPKFKFLQEAEEK----------LRRKMQDDNKGINGNEVDNSLITIIVDKK 254
++ S PPK + + E L R D NKG+ GN+V SL+ I+V+ K
Sbjct: 865 VEKSLPPKIERILRVEMSPLQKQYYKWILERNFHDLNKGVRGNQV--SLLNIVVELK 919
>At5g50780 putative protein
Length = 823
Score = 27.7 bits (60), Expect = 7.2
Identities = 14/54 (25%), Positives = 26/54 (47%)
Query: 134 EESKEDLESEDGKCGKDSRGRSLGDLLDVETPYLTPIASPHFFTPMNCSPYCSP 187
+++++D E + D +G + V + + TP A+P F TP S +P
Sbjct: 580 KDTEKDTEDRESSPEFDPKGSASSRKRTVPSSFKTPTAAPRFNTPTAASEKFNP 633
>At4g11460 serine/threonine kinase-like protein
Length = 700
Score = 27.7 bits (60), Expect = 7.2
Identities = 20/72 (27%), Positives = 37/72 (50%), Gaps = 6/72 (8%)
Query: 186 SPYNQHGFNPLFESTTDAEFNRLKSSPPPKFKFLQEAE-EKLRRKMQDD---NKGINGNE 241
+P N+ + +F+ T++ L PP F F E + LRR ++ D N+ + +
Sbjct: 607 NPVNRPALSTIFQMLTNSSIT-LNVPQPPGFFFRNRPESDTLRRGLEPDQYNNESVTCS- 664
Query: 242 VDNSLITIIVDK 253
+DN+ IT ++ K
Sbjct: 665 IDNATITTLLGK 676
>At4g39790 putative protein
Length = 631
Score = 27.3 bits (59), Expect = 9.4
Identities = 29/106 (27%), Positives = 41/106 (38%), Gaps = 22/106 (20%)
Query: 47 NGNPLK-ELFYMFCWKRPSTSLRQTGFTPPELCSSMRINDNFVHDPQVQTSKEFLFKPYG 105
N PL ++ + WKR S+S T +P +QTSKE G
Sbjct: 291 NVEPLSHQVTKVIVWKRTSSSRSSTS-----------------RNPLIQTSKEDHDDESG 333
Query: 106 EDVIDADYMMQHHH----DGLLGHPRFLFTIVEESKEDLESEDGKC 147
D I+ M+ H D L R L+ V+ S+ + D KC
Sbjct: 334 SDFIEEFCMISGSHSSSLDRLYAWERKLYDEVKASEMIRKEYDRKC 379
>At3g62140 hypothetical protein
Length = 241
Score = 27.3 bits (59), Expect = 9.4
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 14/76 (18%)
Query: 86 NFVHDPQVQTSKEFLFKPYGEDVIDADYMMQHHHDGLLGHPRFLFTIVEESKEDLESEDG 145
NFV + Q+ SK K GE V DG R L+ I++E+K+ ++E
Sbjct: 13 NFVSEEQLDESK----KERGERV----------EDGTFQRDRALYEILKENKDKKDAEFN 58
Query: 146 KCGKDSRGRSLGDLLD 161
+ K S SL +D
Sbjct: 59 ERFKHSESLSLYSSID 74
>At3g58670 unknown protein
Length = 242
Score = 27.3 bits (59), Expect = 9.4
Identities = 19/65 (29%), Positives = 27/65 (41%)
Query: 205 FNRLKSSPPPKFKFLQEAEEKLRRKMQDDNKGINGNEVDNSLITIIVDKKYEREVNHHQC 264
FN KSS P +EA +K+R ++ G E + L+ ER NHH
Sbjct: 9 FNTCKSSLSPNGPVSEEALDKVRNVLEKIKPSDVGLEQEAQLVRNWPGPGNERNGNHHSL 68
Query: 265 HLQQY 269
+Y
Sbjct: 69 PAIKY 73
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,220,178
Number of Sequences: 26719
Number of extensions: 343815
Number of successful extensions: 1015
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 998
Number of HSP's gapped (non-prelim): 16
length of query: 281
length of database: 11,318,596
effective HSP length: 98
effective length of query: 183
effective length of database: 8,700,134
effective search space: 1592124522
effective search space used: 1592124522
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC147536.6