BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147497.9 - phase: 0
(148 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g27890 putative protein 67 5e-12
At2g27280 unknown protein 54 3e-08
At5g53400 unknown protein 53 5e-08
At2g19090 hypothetical protein 35 0.015
At1g30690 putative phosphatidyl-inositol-transfer protein 33 0.056
At3g18590 predicted GPI-anchored protein 33 0.073
At3g53440 unknown protein 31 0.21
At3g43300 guanine nucleotide-exchange - like protein 31 0.21
At3g28510 hypothetical protein 31 0.28
At5g40450 unknown protein 30 0.36
At3g55605 unknown protein 30 0.36
At4g12780 auxilin-like protein 30 0.47
At3g54230 unknown protein 30 0.47
At3g24500 ethylene-responsive transcriptional coactivator, putative 30 0.47
At2g35880 unknown protein 30 0.47
At3g28540 hypothetical protein 30 0.62
At5g60530 late embryonic abundant protein - like 29 0.80
At5g24370 putative protein 29 0.80
At4g27500 unknown protein 29 0.80
At1g48920 unknown protein 29 0.80
>At4g27890 putative protein
Length = 293
Score = 66.6 bits (161), Expect = 5e-12
Identities = 44/116 (37%), Positives = 62/116 (52%), Gaps = 20/116 (17%)
Query: 44 IPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVK-----VKKAKIT 98
+PF+++FDPSNP AFL+ V D I KES +F D+AEK +++A+ K +K K+
Sbjct: 15 VPFTASFDPSNPIAFLEKVLDVIGKES-NFLKKDTAEKEIVAAVMAAKQRLREAEKKKLE 73
Query: 99 VE-------EKMKVDKAAAVAEKKAK-------DVYEKEDEKKDEESGLTAPNQGN 140
E EK K D +K K D E E K+++ESG PN+GN
Sbjct: 74 KESVKSMEVEKPKKDSLKPTELEKPKEESLMATDPMEIEKPKEEKESGPIVPNKGN 129
>At2g27280 unknown protein
Length = 764
Score = 53.9 bits (128), Expect = 3e-08
Identities = 31/141 (21%), Positives = 69/141 (47%), Gaps = 18/141 (12%)
Query: 11 KKSFNVFVTKRIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKES 70
K+ + T + ++ + +T S + +PF+++FDPS+P FL+ VF+F+ K+S
Sbjct: 382 KEITDAVTTAKERLRETEKKTESMDVEKVRPSTLPFNASFDPSDPLGFLEKVFEFVGKKS 441
Query: 71 TSFFDNDSAEKVVLSAI-----------------CTVKVKKAKITVEEKMKVDKAAAVAE 113
+F D A +++A+ TVK+KK + + + ++++
Sbjct: 442 -NFLVKDKAVNAIITAVTDAKERLKEEEKESVKQATVKIKKYGLQIRAPSQKKQSSSRPL 500
Query: 114 KKAKDVYEKEDEKKDEESGLT 134
+ ++ ++DE+ D E ++
Sbjct: 501 LRTASIFGEDDEENDVEKEIS 521
Score = 37.0 bits (84), Expect = 0.004
Identities = 22/51 (43%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
Query: 58 FLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVE-EKMKVDK 107
FL+NV DFI KES +F D+AEK + A+ T K + + + E M V+K
Sbjct: 360 FLENVLDFIGKES-NFLRKDTAEKEITDAVTTAKERLRETEKKTESMDVEK 409
>At5g53400 unknown protein
Length = 304
Score = 53.1 bits (126), Expect = 5e-08
Identities = 40/132 (30%), Positives = 62/132 (46%), Gaps = 29/132 (21%)
Query: 37 QPKSSKPI--PFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAI--CTVKV 92
+ SS+P+ PF +T +NP FL+ VFDF+ ++S F SAE ++ A+ K+
Sbjct: 10 ESSSSRPMIFPFRATLSSANPLGFLEKVFDFLGEQS-DFLKKPSAEDEIVVAVRAAKEKL 68
Query: 93 KKAKITVEEKMKVDKAAAVAEKKAKDVYEK------------------------EDEKKD 128
KKA+ EK V AEK+ + EK ++E++
Sbjct: 69 KKAEKKKAEKESVKPVEKKAEKEIVKLVEKKVEKESVKPTIAASSAEPIEVEKPKEEEEK 128
Query: 129 EESGLTAPNQGN 140
+ESG PN+GN
Sbjct: 129 KESGPIVPNKGN 140
>At2g19090 hypothetical protein
Length = 844
Score = 35.0 bits (79), Expect = 0.015
Identities = 21/51 (41%), Positives = 27/51 (52%), Gaps = 6/51 (11%)
Query: 96 KITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDE------ESGLTAPNQGN 140
K T E + KVDK+ E K +DV E EDE DE +SG N+G+
Sbjct: 276 KATEENRGKVDKSCCNEEVKVEDVDEDEDEDGDEDDDEFTDSGCETENEGD 326
>At1g30690 putative phosphatidyl-inositol-transfer protein
Length = 540
Score = 33.1 bits (74), Expect = 0.056
Identities = 31/112 (27%), Positives = 48/112 (42%), Gaps = 15/112 (13%)
Query: 26 TVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIA------KESTSFFDNDSA 79
TVK + SKP + +A K DF A K++ S +
Sbjct: 28 TVKAVVEETKVEEDESKP-------EGVEKSASFKEESDFFADLKESEKKALSDLKSKLE 80
Query: 80 EKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEK--EDEKKDE 129
E +V + + K K++ E+K +V K A EKK ++ E+ E+EKK E
Sbjct: 81 EAIVDNTLLKTKKKESSPMKEKKEEVVKPEAEVEKKKEEAAEEKVEEEKKSE 132
>At3g18590 predicted GPI-anchored protein
Length = 188
Score = 32.7 bits (73), Expect = 0.073
Identities = 23/73 (31%), Positives = 37/73 (50%), Gaps = 8/73 (10%)
Query: 20 KRIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSA 79
+++IV+ ++T++S+ S P SS SS+ S P A N F + S+S F
Sbjct: 120 QKMIVKVMETESSTESPPPSSSSSSSSSSSLPASTPKAKKSNAFKTAVQFSSSGF----- 174
Query: 80 EKVVLSAICTVKV 92
V+SA+ V V
Sbjct: 175 ---VVSAVLIVSV 184
>At3g53440 unknown protein
Length = 512
Score = 31.2 bits (69), Expect = 0.21
Identities = 20/65 (30%), Positives = 36/65 (54%), Gaps = 7/65 (10%)
Query: 75 DNDSAEKVVLSAICTVKVKKAKITV-EEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGL 133
DN+ E+ V ++C + K+ +I E+ + A ++K K+ E+E++DEE GL
Sbjct: 197 DNEEEEEEV--SVCLTERKRMRIAKPNEEENAEAKADEVKRKGKE----EEEEEDEEEGL 250
Query: 134 TAPNQ 138
A N+
Sbjct: 251 KAKNK 255
>At3g43300 guanine nucleotide-exchange - like protein
Length = 1669
Score = 31.2 bits (69), Expect = 0.21
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 4/60 (6%)
Query: 27 VKTQTSSPSYQPKSSKP--IPFSSTFDPSN--PTAFLKNVFDFIAKESTSFFDNDSAEKV 82
+ T +P PK SK ++T DP + PT L+ ++D I +E D+D+ +K+
Sbjct: 678 LNTDAHNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKKL 737
>At3g28510 hypothetical protein
Length = 530
Score = 30.8 bits (68), Expect = 0.28
Identities = 25/76 (32%), Positives = 35/76 (45%), Gaps = 10/76 (13%)
Query: 75 DNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEK-----EDEKK-- 127
D D K ++ + K K K+ EE+ K + A KKA++ EK EDEKK
Sbjct: 445 DADICIKRLVKTLEEEKEKARKLAEEEEKKKAEKEAKKMKKAEEAEEKKKKTEEDEKKEK 504
Query: 128 ---DEESGLTAPNQGN 140
EE+G + GN
Sbjct: 505 VKAKEENGNVSQQNGN 520
>At5g40450 unknown protein
Length = 2910
Score = 30.4 bits (67), Expect = 0.36
Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
Query: 76 NDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTA 135
N++ E +V + + VK + +K K++ + V+E+ +K V EK +EK +EE L
Sbjct: 839 NETEEILVAKPVSLLDVKS--VEQMQKPKLESPSEVSEETSKTVDEKIEEKPEEEVTLYQ 896
Query: 136 PNQ 138
Q
Sbjct: 897 EGQ 899
>At3g55605 unknown protein
Length = 258
Score = 30.4 bits (67), Expect = 0.36
Identities = 22/100 (22%), Positives = 43/100 (43%), Gaps = 4/100 (4%)
Query: 37 QPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVK--- 93
+P S+ P+S+ +P L V D K+S D+D+ E+ + S+ K++
Sbjct: 44 RPFVSRGFPYSTATEPLKSDQTLIQVIDSEIKDSFEADDHDADEETIDSSDFPFKIEDNP 103
Query: 94 -KAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESG 132
+T+ + ++ + E ED+ D+E G
Sbjct: 104 GHRTVTLTREYNGEQIKVEVSMPGLAMDENEDDVDDDEDG 143
>At4g12780 auxilin-like protein
Length = 904
Score = 30.0 bits (66), Expect = 0.47
Identities = 26/125 (20%), Positives = 54/125 (42%), Gaps = 18/125 (14%)
Query: 30 QTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICT 89
++S S + +P T +N L ++F A +S F D D T
Sbjct: 713 ESSRESLRVPPGQPENLRKTSSVTNIVDDLSSIFGASASQSGGFQDVDGE---------T 763
Query: 90 VKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLT---------APNQGN 140
+ ++A++ ++ + A A+AEK +D+ + ++ + + G+T A +GN
Sbjct: 764 EERRRARLERHQRTQERAAKALAEKNERDLQVQREQVEKDRIGVTLDVEIKRWGAGKEGN 823
Query: 141 LMRVI 145
L ++
Sbjct: 824 LRALL 828
>At3g54230 unknown protein
Length = 1007
Score = 30.0 bits (66), Expect = 0.47
Identities = 24/89 (26%), Positives = 45/89 (49%), Gaps = 10/89 (11%)
Query: 35 SYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKK 94
SY ++ + +P D +N + +N D KE +S +KV++SA T V+K
Sbjct: 619 SYDQQTQQYVPCP---DQNNESKVTENQPDSAKKEKSS------QQKVIISAATTPNVEK 669
Query: 95 AKITVEEKMKVDKAAAVAEKKAKDVYEKE 123
+++ + ++ AAA+A +K + KE
Sbjct: 670 V-LSLPDAVQAAAAAAIASEKREKERVKE 697
>At3g24500 ethylene-responsive transcriptional coactivator, putative
Length = 148
Score = 30.0 bits (66), Expect = 0.47
Identities = 28/110 (25%), Positives = 48/110 (43%), Gaps = 21/110 (19%)
Query: 51 DPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKA----------KITVE 100
DP A L+N +A ++ FD S +K +A+ + KK ++ E
Sbjct: 30 DPKAVNAALRNG---VAVQTVKKFDAGSNKKGKSTAVPVINTKKLEEETEPAAMDRVKAE 86
Query: 101 EKMKVDKAAAVAEKK------AKDVYEKEDEKKDEESGLTAPNQGNLMRV 144
++ + KA EKK AK + E+ ++ E+G PNQ L ++
Sbjct: 87 VRLMIQKARL--EKKMSQADLAKQINERTQVVQEYENGKAVPNQAVLAKM 134
>At2g35880 unknown protein
Length = 432
Score = 30.0 bits (66), Expect = 0.47
Identities = 21/74 (28%), Positives = 34/74 (45%), Gaps = 17/74 (22%)
Query: 92 VKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTA---------PNQGNLM 142
VKK + K++ + + A++K K V ++E EK+ EE TA PN N+
Sbjct: 359 VKKPITKSQPKLETQEKSVKAKEKKKKVKKEEAEKRGEEEKATAVAAKPEEQKPNSNNIQ 418
Query: 143 --------RVIIGG 148
V++GG
Sbjct: 419 VKAEIMASEVVVGG 432
>At3g28540 hypothetical protein
Length = 510
Score = 29.6 bits (65), Expect = 0.62
Identities = 21/77 (27%), Positives = 34/77 (43%), Gaps = 4/77 (5%)
Query: 63 FDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEK 122
+D E T+ DN +K++L K K + + +D +KK +D E+
Sbjct: 265 YDVYDLELTTVKDNSELKKLMLDT----KGKSIVVIEDIDCSLDLTGQRKKKKEEDEDEE 320
Query: 123 EDEKKDEESGLTAPNQG 139
E+EKK E L +G
Sbjct: 321 EEEKKKEAEKLLKRERG 337
Score = 28.1 bits (61), Expect = 1.8
Identities = 22/52 (42%), Positives = 31/52 (59%), Gaps = 5/52 (9%)
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKD--EESGLTAPNQGN 140
K K K+ EEKMK KAA A ++ K E+E +KK+ EE+G + + GN
Sbjct: 460 KEKAKKLAEEEKMK--KAARDA-RRIKKKAEEEHKKKNKVEENGDVSHDNGN 508
>At5g60530 late embryonic abundant protein - like
Length = 439
Score = 29.3 bits (64), Expect = 0.80
Identities = 17/40 (42%), Positives = 23/40 (57%)
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
K +K K EEK K DK EKK K EK+D+++ E+
Sbjct: 75 KKEKEKKDKEEKEKKDKERKEKEKKDKLEKEKKDKERKEK 114
Score = 28.9 bits (63), Expect = 1.0
Identities = 15/32 (46%), Positives = 22/32 (67%), Gaps = 1/32 (3%)
Query: 100 EEKMKVDKAAAVAEKKAKDVYEKED-EKKDEE 130
+E+ K DK A +KK K+ +KE+ EKKD+E
Sbjct: 61 KEQEKKDKEKAAKDKKEKEKKDKEEKEKKDKE 92
Score = 26.9 bits (58), Expect = 4.0
Identities = 17/37 (45%), Positives = 21/37 (55%), Gaps = 4/37 (10%)
Query: 93 KKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDE 129
+KA +EK K DK EK+ KD KE EKKD+
Sbjct: 69 EKAAKDKKEKEKKDKE----EKEKKDKERKEKEKKDK 101
Score = 26.6 bits (57), Expect = 5.2
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 3/48 (6%)
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKE---DEKKDEESGLTA 135
K K + E+K K++K E+K K+ EKE EKKD+E A
Sbjct: 88 KKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKEESEAA 135
>At5g24370 putative protein
Length = 191
Score = 29.3 bits (64), Expect = 0.80
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 6/55 (10%)
Query: 44 IPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKIT 98
+PFSST +P PT K++ D I ++ +F + E +V S + + A IT
Sbjct: 22 LPFSSTVNPDKPT---KSIIDRICNQTVNF---NECELIVTSQLHSPHANIATIT 70
>At4g27500 unknown protein
Length = 612
Score = 29.3 bits (64), Expect = 0.80
Identities = 20/51 (39%), Positives = 30/51 (58%), Gaps = 2/51 (3%)
Query: 91 KVKKAKITVEEKMKV-DKAAAVAEKKAKDVYEKEDEKKDEESGLTAPNQGN 140
++ KAK +E K K+ +KAAA A +A+ EK+ EKK++E GN
Sbjct: 479 EIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKK-EKKEQEKKAKKKTGGN 528
>At1g48920 unknown protein
Length = 557
Score = 29.3 bits (64), Expect = 0.80
Identities = 34/118 (28%), Positives = 44/118 (36%), Gaps = 18/118 (15%)
Query: 39 KSSKPIPFSSTFDPSN--------PTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTV 90
K SK SS D S+ P A KN KES+S D+ S ++ +
Sbjct: 117 KKSKDDSSSSDDDSSDEEVAVTKKPAAAAKNGSVKAKKESSSEDDSSSEDEPAKKPAAKI 176
Query: 91 KVKKAKITV----------EEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTAPNQ 138
AK + E++ K AA A KA + DE DEES P Q
Sbjct: 177 AKPAAKDSSSSDDDSDEDSEDEKPATKKAAPAAAKAASSSDSSDEDSDEESEDEKPAQ 234
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.311 0.126 0.336
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,204,717
Number of Sequences: 26719
Number of extensions: 131629
Number of successful extensions: 1213
Number of sequences better than 10.0: 94
Number of HSP's better than 10.0 without gapping: 45
Number of HSP's successfully gapped in prelim test: 50
Number of HSP's that attempted gapping in prelim test: 1043
Number of HSP's gapped (non-prelim): 177
length of query: 148
length of database: 11,318,596
effective HSP length: 90
effective length of query: 58
effective length of database: 8,913,886
effective search space: 517005388
effective search space used: 517005388
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC147497.9