BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147406.5 - phase: 0 /pseudo
(99 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g32110 non-LTR reverse transcriptase, putative 59 5e-10
At5g10850 putative protein 58 9e-10
At2g07670 putative non-LTR retrolelement reverse transcriptase 57 2e-09
At4g15600 hypothetical protein 56 3e-09
At2g07740 putative Ta11-like non-LTR retroelement protein 52 4e-08
At2g01050 hypothetical protein 52 4e-08
At4g08820 putative protein 35 0.006
At4g07521 putative protein 32 0.054
At1g06850 bZip transcription factor AtbZip52 29 0.45
At4g10830 putative protein 28 0.59
At2g01840 putative non-LTR retroelement reverse transcriptase 28 0.59
At5g28545 putative protein 28 0.77
At4g31400 unknown protein 27 2.3
At5g23020 2-isopropylmalate synthase-like protein 26 2.9
At3g32360 hypothetical protein 26 2.9
At2g25550 putative non-LTR retroelement reverse transcriptase 25 6.6
At2g03150 Unknown protein (T18E12.18; At2g03150) 25 6.6
At5g43120 putative protein 25 8.6
At3g54840 small GTPase Ara6 (ARA6) 25 8.6
At2g15520 putative Ta11-like non-LTR retroelement protein 25 8.6
>At3g32110 non-LTR reverse transcriptase, putative
Length = 1911
Score = 58.5 bits (140), Expect = 5e-10
Identities = 24/69 (34%), Positives = 40/69 (57%)
Query: 31 VKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFD 90
VK+LG+ + A+ RL++ W+ G ++D F+MV+F++ + +TGGPW F
Sbjct: 100 VKVLGRKVSLAAVSHRLREMWKPSGAMYVLDLPRHFFMVRFEKEEEFLTALTGGPWRAFG 159
Query: 91 HCLAVTHWT 99
CL V W+
Sbjct: 160 SCLLVKAWS 168
>At5g10850 putative protein
Length = 181
Score = 57.8 bits (138), Expect = 9e-10
Identities = 22/70 (31%), Positives = 41/70 (58%)
Query: 30 VVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIF 89
+VK+ G+N+ A+ +L++ W+ +G ++D F+M++F+ + +TGGPW F
Sbjct: 98 IVKVPGRNMTISALSRKLREMWKPRGAMSVVDLPRKFFMIRFEAEEEYMAALTGGPWRAF 157
Query: 90 DHCLAVTHWT 99
D L V W+
Sbjct: 158 DRYLMVQAWS 167
>At2g07670 putative non-LTR retrolelement reverse transcriptase
Length = 913
Score = 56.6 bits (135), Expect = 2e-09
Identities = 24/70 (34%), Positives = 40/70 (56%)
Query: 30 VVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIF 89
VVK+LG+N+ + RL++ W+ QG ++D F+M++F+ + +TGGPW F
Sbjct: 57 VVKVLGRNISIANLNRRLREMWKPQGAMFVVDLPCQFFMIRFEREDEYLSALTGGPWRAF 116
Query: 90 DHCLAVTHWT 99
L V W+
Sbjct: 117 GSYLLVQAWS 126
>At4g15600 hypothetical protein
Length = 655
Score = 56.2 bits (134), Expect = 3e-09
Identities = 23/70 (32%), Positives = 38/70 (53%)
Query: 30 VVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIF 89
+VK+LG++ + +L+ WR GG ++D F+MV+F+ D +TGGPW +
Sbjct: 97 LVKILGRHTTIEVLSRKLRDLWRPTGGMSVLDLPRQFFMVRFEVEEDYMMALTGGPWRVL 156
Query: 90 DHCLAVTHWT 99
L V W+
Sbjct: 157 GSILMVQAWS 166
>At2g07740 putative Ta11-like non-LTR retroelement protein
Length = 409
Score = 52.4 bits (124), Expect = 4e-08
Identities = 22/70 (31%), Positives = 37/70 (52%)
Query: 30 VVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIF 89
VVK+LG+++ + RL++ W+ GG +++ F+MVKFD D G PW +
Sbjct: 34 VVKVLGRHITIADLNRRLRELWKPNGGMSVLNLPRSFFMVKFDLEEDYLAAAIGDPWRVL 93
Query: 90 DHCLAVTHWT 99
L + W+
Sbjct: 94 GSILMIRAWS 103
>At2g01050 hypothetical protein
Length = 515
Score = 52.4 bits (124), Expect = 4e-08
Identities = 20/70 (28%), Positives = 38/70 (53%)
Query: 30 VVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIF 89
+VK+LG + + +L++ W+ G +MD F+M++F+ + +TGGPW +
Sbjct: 82 IVKVLGSQIPISVLNRKLRELWKPSGVMTVMDLPRQFFMIRFELEEEYMAALTGGPWRVL 141
Query: 90 DHCLAVTHWT 99
+ L V W+
Sbjct: 142 GNYLLVQDWS 151
>At4g08820 putative protein
Length = 404
Score = 35.0 bits (79), Expect = 0.006
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 9/53 (16%)
Query: 56 GFEIMDNDNG---------FYMVKFDEAADKEKVITGGPWLIFDHCLAVTHWT 99
G E+++ NG F+M++F+ + +TGGPW +F + L V W+
Sbjct: 46 GEEVLEAMNGLWKRCMIVKFFMIRFELEEEYLAALTGGPWRVFGNYLMVQDWS 98
>At4g07521 putative protein
Length = 223
Score = 32.0 bits (71), Expect = 0.054
Identities = 13/39 (33%), Positives = 20/39 (50%)
Query: 60 MDNDNGFYMVKFDEAADKEKVITGGPWLIFDHCLAVTHW 98
MD F+M++F+ + +TG PW +F L V W
Sbjct: 1 MDLPRQFFMIRFELQEEYLAALTGDPWRVFGSNLTVQAW 39
>At1g06850 bZip transcription factor AtbZip52
Length = 337
Score = 28.9 bits (63), Expect = 0.45
Identities = 12/28 (42%), Positives = 21/28 (74%)
Query: 1 MRMEIVFYPRFISNNQSFNNYVRHGRMQ 28
M+M F P +SN+QS ++++++GRMQ
Sbjct: 282 MQMHSSFNPMEMSNSQSVSDFLQNGRMQ 309
>At4g10830 putative protein
Length = 1294
Score = 28.5 bits (62), Expect = 0.59
Identities = 22/94 (23%), Positives = 32/94 (33%), Gaps = 5/94 (5%)
Query: 5 IVFYPRFISNNQSFNNYVRHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDN 64
IV P F+S S N + V + + A+ ++ + W N
Sbjct: 21 IVLSPEFVSQAASVNRF-----SLIVTTVNPRKQNLRALIGQMPRVWGLVDSCVGRIVGN 75
Query: 65 GFYMVKFDEAADKEKVITGGPWLIFDHCLAVTHW 98
G KF V+ GPW D L+V W
Sbjct: 76 GRVQFKFRNEESMNLVLQRGPWSFNDWMLSVHRW 109
>At2g01840 putative non-LTR retroelement reverse transcriptase
Length = 1715
Score = 28.5 bits (62), Expect = 0.59
Identities = 16/67 (23%), Positives = 24/67 (34%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFDHC 92
L+ +N + + +TW G N + F + V+ GPW D
Sbjct: 44 LMPRNQNLRQILTTVPRTWGLVGFVRGRIIQNRRFQFIFPSEESLDTVLRRGPWSFADRM 103
Query: 93 LAVTHWT 99
L V WT
Sbjct: 104 LVVERWT 110
>At5g28545 putative protein
Length = 477
Score = 28.1 bits (61), Expect = 0.77
Identities = 14/52 (26%), Positives = 22/52 (41%)
Query: 47 LQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFDHCLAVTHW 98
L W +G D NG + +FD D +KV+ P+ I + + W
Sbjct: 63 LSNRWNLKGKAIGSDLGNGCFQFRFDFEEDLQKVLANRPYHIDQWMVILQRW 114
>At4g31400 unknown protein
Length = 322
Score = 26.6 bits (57), Expect = 2.3
Identities = 22/90 (24%), Positives = 38/90 (41%), Gaps = 17/90 (18%)
Query: 14 NNQSFNNYVRHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQGGFE-----------IMDN 62
N+QSF+ +G + F +LL K LG + + W+ + F + +N
Sbjct: 115 NHQSFHKDYMYG-LPFK-RLLDKLLGVYIWSQG----WQNEKAFTSPLFIKNRIVMVSEN 168
Query: 63 DNGFYMVKFDEAADKEKVITGGPWLIFDHC 92
D+ + K E +V G W++ HC
Sbjct: 169 DSPAHRNKVQEVVKMMEVELGEDWILHQHC 198
>At5g23020 2-isopropylmalate synthase-like protein
Length = 503
Score = 26.2 bits (56), Expect = 2.9
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK G HA+++RL++ G+EI D ++ E +K IT
Sbjct: 422 VLGKLSGRHAVKDRLKEL-----GYEISDEKFNDIFSRYRELTKDKKRIT 466
>At3g32360 hypothetical protein
Length = 551
Score = 26.2 bits (56), Expect = 2.9
Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
Query: 47 LQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFDHCLAV 95
+ W +G D G + +FD D +KV+ P+ FDH + +
Sbjct: 63 ISNRWNLKGKAVGSDLGRGCFQFRFDYEEDLQKVLENKPY-HFDHWMVI 110
>At2g25550 putative non-LTR retroelement reverse transcriptase
Length = 1750
Score = 25.0 bits (53), Expect = 6.6
Identities = 11/36 (30%), Positives = 14/36 (38%)
Query: 63 DNGFYMVKFDEAADKEKVITGGPWLIFDHCLAVTHW 98
D G KF V+ GPW D L++ W
Sbjct: 74 DKGRVQFKFQSEEAMNLVLRRGPWSFNDWMLSIHRW 109
>At2g03150 Unknown protein (T18E12.18; At2g03150)
Length = 1336
Score = 25.0 bits (53), Expect = 6.6
Identities = 14/39 (35%), Positives = 21/39 (52%), Gaps = 2/39 (5%)
Query: 10 RFISNNQSFNNYVRHGRMQFVVKLLGKNLGYHAMRERLQ 48
RF NQ+ YVR M+ + LGK L + ++E +Q
Sbjct: 1276 RFFDRNQA--GYVRVEDMRVTIHSLGKFLSHREVKELVQ 1312
>At5g43120 putative protein
Length = 588
Score = 24.6 bits (52), Expect = 8.6
Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 6/49 (12%)
Query: 6 VFYPRFISNNQSFNNYVRHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQ 54
V Y F+S + + GR+++ LL + LG M +R + W +Q
Sbjct: 194 VVYEEFVSVQE------KEGRVRYHSDLLTRGLGGLEMEDRKAEEWASQ 236
>At3g54840 small GTPase Ara6 (ARA6)
Length = 202
Score = 24.6 bits (52), Expect = 8.6
Identities = 14/54 (25%), Positives = 24/54 (43%), Gaps = 5/54 (9%)
Query: 23 RHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAAD 76
+HG V+ L+G H RE T+ G E+ + + F++ + AD
Sbjct: 134 KHGSPDIVMALVGNKADLHEKRE-----VPTEDGMELAEKNGMFFIETSAKTAD 182
>At2g15520 putative Ta11-like non-LTR retroelement protein
Length = 483
Score = 24.6 bits (52), Expect = 8.6
Identities = 12/52 (23%), Positives = 23/52 (44%)
Query: 47 LQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFDHCLAVTHW 98
+ + W+ +G + + +N + FD D E+V+ G D + V W
Sbjct: 54 MPRKWQKEGKVKGIALNNERFQFIFDSEHDLEEVLAKGVHTFNDWSIVVDRW 105
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.327 0.141 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,429,341
Number of Sequences: 26719
Number of extensions: 92973
Number of successful extensions: 218
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 202
Number of HSP's gapped (non-prelim): 23
length of query: 99
length of database: 11,318,596
effective HSP length: 75
effective length of query: 24
effective length of database: 9,314,671
effective search space: 223552104
effective search space used: 223552104
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC147406.5