BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147014.3 + phase: 0 
         (205 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At3g18030 HAL3A protein                                               220  4e-58
At1g48605 regulatory protein HAL3B                                    213  6e-56
At2g01600 unknown protein                                              31  0.52
At3g11130 hypothetical protein                                         29  2.0
At5g22940 putative protein                                             27  5.7
At4g18050 multidrug resistance protein/P-glycoprotein - like           27  5.7
At1g75010 unknown protein                                              27  7.5
At1g19430 unknown protein (F18O14.20)                                  27  7.5
At3g23440 hypothetical protein                                         27  9.7
At1g75730 hypothetical protein                                         27  9.7

>At3g18030 HAL3A protein
          Length = 209

 Score =  220 bits (561), Expect = 4e-58
 Identities = 108/187 (57%), Positives = 138/187 (73%), Gaps = 8/187 (4%)

Query: 17  PRKPRILLGCCGSVAAMKFGLVFNAFSEWAEVRAVVTETSLQFLVHEKAESLFTHRHDIY 76
           PRKPR+LL   GSVAA+KFG + + F+EWAEVRAVVT++SL FL         T    +Y
Sbjct: 17  PRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFLDKLSLPQEVT----LY 72

Query: 77  KDDSEWKNWKKIGDSVLHIELANWADIMVIAPLSAHTAAKIAGGLCDNLLTSIVRAWDYE 136
            D+ EW +W KIGD VLHIEL  WAD++VIAPLSA+T  KIAGGLCDNLLT I+RAWDY 
Sbjct: 73  TDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYT 132

Query: 137 KPMFVAPSMDGCMWRNPFTEQNFMSIEELGVTLIPPVQHM----PTNMREMADPSTIFST 192
           KP+FVAP+M+  MW NPFTE++ +S++ELG+TLIPP++            MA+PS I+ST
Sbjct: 133 KPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEPSLIYST 192

Query: 193 VKSFYDS 199
           V+ F++S
Sbjct: 193 VRLFWES 199


>At1g48605 regulatory protein HAL3B
          Length = 201

 Score =  213 bits (542), Expect = 6e-56
 Identities = 105/192 (54%), Positives = 137/192 (70%), Gaps = 8/192 (4%)

Query: 18  RKPRILLGCCGSVAAMKFGLVFNAFSEWAEVRAVVTETSLQFLVHEKAESLFTHRHDIYK 77
           RKPRILL   GSVA++KF  + + FSEWAEV+AV +++SL F+         T    +Y 
Sbjct: 10  RKPRILLAASGSVASIKFSNLCHCFSEWAEVKAVASKSSLNFVDKPSLPQNVT----LYT 65

Query: 78  DDSEWKNWKKIGDSVLHIELANWADIMVIAPLSAHTAAKIAGGLCDNLLTSIVRAWDYEK 137
           D+ EW +W KIGD VLHIEL  WAD+M+IAPLSA+T AKIAGGLCDNLLT IVRAWDY K
Sbjct: 66  DEDEWSSWNKIGDPVLHIELRRWADVMIIAPLSANTLAKIAGGLCDNLLTCIVRAWDYSK 125

Query: 138 PMFVAPSMDGCMWRNPFTEQNFMSIEELGVTLIPPVQHM----PTNMREMADPSTIFSTV 193
           P+FVAP+M+  MW NPFTE++ + ++ELG+TLIPP++            MA+PS I+STV
Sbjct: 126 PLFVAPAMNTLMWNNPFTERHLVLLDELGITLIPPIKKKLACGDYGNGAMAEPSLIYSTV 185

Query: 194 KSFYDSNILKDK 205
           + F++S   K +
Sbjct: 186 RLFWESQARKQR 197


>At2g01600 unknown protein
          Length = 571

 Score = 30.8 bits (68), Expect = 0.52
 Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 6/65 (9%)

Query: 100 WADIMVIAP---LSAHTAAKIAGGLCDNLLTSIVRAWDY---EKPMFVAPSMDGCMWRNP 153
           W   +V AP   +SA T  K+AGGL    L+S+     Y   ++P++ AP+ +     +P
Sbjct: 425 WELALVTAPSSDISASTERKLAGGLDTLTLSSLYDDGAYIASQRPVYGAPAPNPFASHDP 484

Query: 154 FTEQN 158
           F   N
Sbjct: 485 FASSN 489


>At3g11130 hypothetical protein
          Length = 1673

 Score = 28.9 bits (63), Expect = 2.0
 Identities = 23/84 (27%), Positives = 36/84 (42%), Gaps = 9/84 (10%)

Query: 120 GLCDNLLTSIVRAWDYEK--PMFVAPSMDGCMWRNPFTEQNFMSIEELGVTLIPPVQHMP 177
           G CD  L ++       K    +V   MDG +W    TE+N     E    LI  V  + 
Sbjct: 906 GQCDEELINVTNKNSLFKLQARYVVERMDGDLWEKVLTEEN-----EYRRQLIDQV--VS 958

Query: 178 TNMREMADPSTIFSTVKSFYDSNI 201
           T + E   P  + + VK+F  +++
Sbjct: 959 TALPESKSPEQVSAAVKAFMTADL 982


>At5g22940 putative protein
          Length = 498

 Score = 27.3 bits (59), Expect = 5.7
 Identities = 26/97 (26%), Positives = 41/97 (41%), Gaps = 12/97 (12%)

Query: 16  APRKPRIL----LGCCGSVAAMKFGLVFNAFSEWAEVRAVVTETSL----QFLVHEKAES 67
           AP  PR++    LGC   V A    L F+   +W E+   V E  +    + L H  A +
Sbjct: 381 APWSPRLVESAVLGCVPVVIADGIQLPFSETVQWPEISLTVAEKDVRNLRKVLEHVAATN 440

Query: 68  LFTHRHD----IYKDDSEWKNWKKIGDSVLHIELANW 100
           L   + +    ++K    +    K GD+  HI  + W
Sbjct: 441 LSAIQRNLHEPVFKRALLYNVPMKEGDATWHILESLW 477


>At4g18050 multidrug resistance protein/P-glycoprotein - like
          Length = 1323

 Score = 27.3 bits (59), Expect = 5.7
 Identities = 15/36 (41%), Positives = 20/36 (54%), Gaps = 3/36 (8%)

Query: 7   SKSAIGHVNAPRKPRILLGCCGSVAAMKFGLVFNAF 42
           S   + H+N P  P ++LG   S+AAM  G VF  F
Sbjct: 671 SLKRLAHLNKPEIPVLVLG---SIAAMVHGTVFPIF 703


>At1g75010 unknown protein
          Length = 717

 Score = 26.9 bits (58), Expect = 7.5
 Identities = 18/49 (36%), Positives = 26/49 (52%), Gaps = 1/49 (2%)

Query: 92  VLHIELANWADIMVIAPLSAHTAAKIAGGLCDNLLTSIVRAWDYEKPMF 140
           V+H +L       V   L ++  AK+  G+  NL TSI+RA  Y+ P F
Sbjct: 255 VMHKDLKELEGSEVKTILESYKEAKVGFGVGHNLKTSILRA-IYDCPFF 302


>At1g19430 unknown protein (F18O14.20)
          Length = 724

 Score = 26.9 bits (58), Expect = 7.5
 Identities = 22/80 (27%), Positives = 37/80 (45%), Gaps = 12/80 (15%)

Query: 22  ILLGCCG-------SVAAMKFGLVFNAFSEWAEVRAVVTETSLQFLVHEKAESLFTHRHD 74
           ++LG C        S +A  F +   +F + AE  +  T+++     HE +ES   H   
Sbjct: 31  VVLGLCFVFFWSFLSSSASTFNVQRESFDDIAEPVSSRTKSA-----HEVSESSKLHERG 85

Query: 75  IYKDDSEWKNWKKIGDSVLH 94
             +  S+ K  KK+G S +H
Sbjct: 86  KVESGSKSKEGKKVGGSSVH 105


>At3g23440 hypothetical protein
          Length = 108

 Score = 26.6 bits (57), Expect = 9.7
 Identities = 15/53 (28%), Positives = 26/53 (48%), Gaps = 6/53 (11%)

Query: 27 CGSVAAMKFGLVFNAFSEWAEVRAVVTETSLQFLVHEKAESLFTHRHDIYKDD 79
          CG++   K       +S WAEV  +V+ + LQ    +KA   ++    ++K D
Sbjct: 43 CGTMLGRKL------YSGWAEVGPLVSPSPLQSREVKKARQSYSEVASVFKTD 89


>At1g75730 hypothetical protein
          Length = 565

 Score = 26.6 bits (57), Expect = 9.7
 Identities = 32/115 (27%), Positives = 46/115 (39%), Gaps = 23/115 (20%)

Query: 4   NSDSKSAIGHVNAPRKPRILLGCCGSVAAMKFGLV---FNAF-----------SEWAEVR 49
           N    + + H + PRK R  +      +  K   V    N F           S+  E +
Sbjct: 55  NLKDTNGVDHASVPRKLRSAMKKRNLESVSKLSSVSKRLNRFKTGTESSVKKESQEMEAK 114

Query: 50  AVVTETSLQFLVHEK--AESL------FTHRHDIYKDDSEWKNWKKIGDSVLHIE 96
           A+VTE S+     EK  AE+L      FT    + K  S  K   K+ DS+L +E
Sbjct: 115 AIVTE-SMMISKDEKEVAETLYGLAGMFTETDSLGKKTSNEKETSKVVDSILVVE 168


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.321    0.133    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,678,293
Number of Sequences: 26719
Number of extensions: 179550
Number of successful extensions: 347
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 341
Number of HSP's gapped (non-prelim): 10
length of query: 205
length of database: 11,318,596
effective HSP length: 95
effective length of query: 110
effective length of database: 8,780,291
effective search space: 965832010
effective search space used: 965832010
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)