BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.12 + phase: 0
(750 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g15840 unknown protein 370 e-102
At1g11100 transcription factor RUSH-1alpha isolog 36 0.069
At5g14540 unknown protein 33 0.45
At1g34360 transcription factor like protein 32 1.3
At5g51760 protein phosphatase-2C; PP2C-like protein 32 1.7
At4g38190 unknown protein 31 2.2
At3g49510 putative protein 31 2.2
At1g23540 putative serine/threonine protein kinase 31 2.2
At3g50580 proline-rich protein 30 3.8
At2g36810 unknown protein 30 5.0
At5g40790 putative protein 30 6.5
At5g13600 photoreceptor-interacting protein-like; non-phototropi... 30 6.5
At1g55560 unknown protein 30 6.5
At5g46460 putative protein 29 8.5
At3g49900 putative protein 29 8.5
At3g22080 hypothetical protein 29 8.5
>At4g15840 unknown protein
Length = 298
Score = 370 bits (950), Expect = e-102
Identities = 205/345 (59%), Positives = 233/345 (67%), Gaps = 49/345 (14%)
Query: 408 MPGGGGNIYVAESSTGERNGSFRPVRVHVRGA-IDGLAGIGRGTTFVPAAASPPTRFVFS 466
M G N+YV ESS G+ G F+PVR+ VRG IDGL GIG TFVP A PPT FV+S
Sbjct: 1 MMGDMDNVYVPESSQGDGIGIFKPVRISVRGQHIDGLTGIGCEATFVPPPAWPPTPFVYS 60
Query: 467 RVPFGVGNRNYPQSAANDDSEARADHNGDLSGDGLTALVGLSQG-GNYGTNIHTELTQRE 525
RVP NRN QS A+D SE R D +G++S DGLTALVGLSQG G N E T+
Sbjct: 61 RVPI---NRNGQQSIASDGSEGRIDQSGEISKDGLTALVGLSQGTSGVGNNPRGEQTE-- 115
Query: 526 QEMGLQSTAGGASTGGIPVQLLETPEHTIGIEWENDNSSSISLDLKTPLSHFPPFRFGVS 585
G G V + E E ++G EWEN S +ISLD KTPL HFPPFRFGV
Sbjct: 116 ----------GGRGSGATVGMSEPKEPSVGTEWENA-SCTISLDTKTPLCHFPPFRFGVE 164
Query: 586 FEEVHRLGDGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTLGNVFITSKLSPSGCEQI 645
FE+VHRL +G V+HSPE FYAGSLWK+SIQAFNDEDPQGRRT+
Sbjct: 165 FEDVHRLANGHVEHSPEFFYAGSLWKISIQAFNDEDPQGRRTI----------------- 207
Query: 646 SAIVIIVNSLNDNAGLFLHRRKAEITDVHRKVHMYVDSREKVTARYQLTCPSKREMLVFG 705
GLFLHRR+AEI D RKVH+Y+D R+KVTARYQL CPSKRE+++FG
Sbjct: 208 --------------GLFLHRREAEIVDSVRKVHVYIDPRDKVTARYQLICPSKREVMLFG 253
Query: 706 SFKQTGTLLPKAPKGWGWRTALLFDELADLLQNGALRVIAVVQLV 750
SFKQ GTLLPKAPKGWGWRTALLFDEL++LLQNGALRV AVVQLV
Sbjct: 254 SFKQRGTLLPKAPKGWGWRTALLFDELSELLQNGALRVAAVVQLV 298
>At1g11100 transcription factor RUSH-1alpha isolog
Length = 1227
Score = 36.2 bits (82), Expect = 0.069
Identities = 53/235 (22%), Positives = 92/235 (38%), Gaps = 33/235 (14%)
Query: 35 DPTTAELRALDCNLASLC-EHVQIEGFNSGSFSDIVVNAMGSTYHLHRLILSRSSYFRFE 93
DP T+ +LD + E+++ FN SFS V ++ S R S + E
Sbjct: 413 DPETSRTESLDYLVGDEDHEYIKRTCFNLSSFSSGTVESLSSKRIPERE--DDSEIHKIE 470
Query: 94 LAFHTFLAKSAHCKVEHPAHGIPGSESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLK 153
++ F+ + V+ P S+++ G + C E++ G M+
Sbjct: 471 -SYGEFVNPHQYLPVQRPVFS-----------SEHSTGSQTLNNCGGLKFESNKGNMNFH 518
Query: 154 SDLKDTSMPNLLVELGDSEGDFNSDICDSNQRVQLASYDISPNLNPSYPSDMEGPSLGNS 213
+DL+D S + E +G + +QR+ L+ S P G
Sbjct: 519 ADLQDLSQHS--SEASPPDGVLAVSLL-RHQRIALSWM--------SQKETSGNPCFGGI 567
Query: 214 LSDPDGV-RTSCYVEVPLGAGTTTGVGIEGPSEEGSCYHSD------NNNRLVRD 261
L+D G+ +T + + L +T + E S+ G C SD N N++V D
Sbjct: 568 LADDQGLGKTVSTIALILTERSTPYLPCEEDSKNGGCNQSDHSQVVFNENKVVED 622
>At5g14540 unknown protein
Length = 530
Score = 33.5 bits (75), Expect = 0.45
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 14/50 (28%)
Query: 2 ENPQYPNNPPPS------------PYNQRS--ASIPKQPPSHHSDNNDPT 37
+ PQYP PPP PY Q+S + P+QPPSH + P+
Sbjct: 316 QQPQYPQQPPPQLQHPSGYNPEEPPYPQQSYPPNPPRQPPSHPPPGSAPS 365
>At1g34360 transcription factor like protein
Length = 520
Score = 32.0 bits (71), Expect = 1.3
Identities = 14/34 (41%), Positives = 17/34 (49%)
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDP 36
NPQ+PN P P ++ P Q PS S N P
Sbjct: 416 NPQFPNQRPSPPQSRFPDQAPNQQPSGPSPNRHP 449
>At5g51760 protein phosphatase-2C; PP2C-like protein
Length = 416
Score = 31.6 bits (70), Expect = 1.7
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Query: 362 QRVQEIAADTCRVCSLMTMSCTCHRQFAFLHGSTTGSYIQEHNH 405
+R+ E+A TC VC C C + A + GST + + H+H
Sbjct: 201 KRMDEMATSTC-VCGTSVPLCNCDPREAAISGSTAVTAVLTHDH 243
>At4g38190 unknown protein
Length = 1111
Score = 31.2 bits (69), Expect = 2.2
Identities = 25/87 (28%), Positives = 40/87 (45%), Gaps = 13/87 (14%)
Query: 179 ICDSNQRVQLA----SYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGT 234
+C N+ L +D+ NP+ SD+ G L S +DP+ E PL
Sbjct: 323 LCPINRSTDLEVLRDKFDMPSPSNPTGRSDLPGIDLFVSTADPE-------KEPPLVTAN 375
Query: 235 T--TGVGIEGPSEEGSCYHSDNNNRLV 259
T + + ++ P E+ SCY SD+ L+
Sbjct: 376 TILSILAVDYPVEKVSCYLSDDGGALL 402
>At3g49510 putative protein
Length = 662
Score = 31.2 bits (69), Expect = 2.2
Identities = 19/64 (29%), Positives = 34/64 (52%), Gaps = 4/64 (6%)
Query: 633 ITSKLSPSGCEQISAIVI---IVNSLNDNAGLFLHRRKAEITDVHRKVHMYVDSREKVTA 689
I++K+ P+ S + + +VN L D+ ++ K+ D +KV + DS+E T
Sbjct: 284 ISTKIEPNAVSWSSFLTVDMSLVNGLPDHFSMYFEA-KSFFIDEEKKVVVLFDSKEIKTC 342
Query: 690 RYQL 693
RYQ+
Sbjct: 343 RYQM 346
>At1g23540 putative serine/threonine protein kinase
Length = 731
Score = 31.2 bits (69), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Query: 7 PNNPPP----SPYNQRSASIPKQPPSHHSDNNDPTTAELRA 43
P+ PPP SP + P PP+ +DNN P + +L++
Sbjct: 92 PSPPPPTSNESPSPPEDSETPPAPPNESNDNNPPPSQDLQS 132
>At3g50580 proline-rich protein
Length = 265
Score = 30.4 bits (67), Expect = 3.8
Identities = 13/32 (40%), Positives = 18/32 (55%), Gaps = 5/32 (15%)
Query: 2 ENPQYPNNPPPSPYNQRSASIPKQPPSHHSDN 33
++P P+ PPP+P P PPSHHS +
Sbjct: 141 KSPSTPSLPPPTPKKS-----PPPPPSHHSSS 167
>At2g36810 unknown protein
Length = 1071
Score = 30.0 bits (66), Expect = 5.0
Identities = 32/128 (25%), Positives = 52/128 (40%), Gaps = 11/128 (8%)
Query: 627 TLGNVFITSKLSPSGCEQISA--IVIIVNSLNDNAGLFLHRRKAEITDVHRKVHMYVDS- 683
+L N+ S LS SG + + I++++L G+ R++ V RK + V
Sbjct: 38 SLHNLLNASLLSESGPPLLDFEDLTIVLSTLLPVIGINNERKRFSDISVGRKTYNEVQRC 97
Query: 684 --------REKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALLFDELADL 735
E + C K + L FG+ LLP+ + W + LL D + L
Sbjct: 98 FLTVGLVYPEDLFTFLLNKCKLKEDPLTFGALCILKHLLPRLFEAWHSKRPLLVDTASSL 157
Query: 736 LQNGALRV 743
L +L V
Sbjct: 158 LDEQSLAV 165
>At5g40790 putative protein
Length = 303
Score = 29.6 bits (65), Expect = 6.5
Identities = 27/97 (27%), Positives = 40/97 (40%), Gaps = 10/97 (10%)
Query: 99 FLAKSAHCKVEHPAHGIPGSESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLKSDLKD 158
FL ++ K ++ + SG+ D T G +GC ++TDL K D D
Sbjct: 52 FLGRNRRSKRKYVRVRDKKNSSGSNNKKDIT---GKKNGCDRGNIKTDLDKKIDGDDRTD 108
Query: 159 TSMPNLLVEL-------GDSEGDFNSDICDSNQRVQL 188
LL E GD G+F SD+ D + + L
Sbjct: 109 IVTLQLLPEKDRDIGNNGDKAGEFCSDLSDMDPKKSL 145
>At5g13600 photoreceptor-interacting protein-like; non-phototropic
hypocotyl-like protein
Length = 591
Score = 29.6 bits (65), Expect = 6.5
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 7/57 (12%)
Query: 62 SGSFSDIVVNAMGSTYHLHRL-ILSRSSYFRFELAFHTFLAKSAHCKVEHPAHGIPG 117
+G D+++ + ++HLH+ +LSRS Y T +K++ H IPG
Sbjct: 24 TGLKPDVMIQVVDESFHLHKFPLLSRSGY------LETLFSKASETTCVAQLHDIPG 74
>At1g55560 unknown protein
Length = 549
Score = 29.6 bits (65), Expect = 6.5
Identities = 12/19 (63%), Positives = 12/19 (63%)
Query: 705 GSFKQTGTLLPKAPKGWGW 723
GS Q T LPKAP GW W
Sbjct: 299 GSNVQASTELPKAPVGWAW 317
>At5g46460 putative protein
Length = 697
Score = 29.3 bits (64), Expect = 8.5
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 6/70 (8%)
Query: 256 NRLVRDQSRDSFSSSSCSGLTSSEWGR--YGTPL---LSWGGHVGRRQVKAH-PRGNYRD 309
N ++ +QS + +SCS L + +WG+ +G + L VG V + GN D
Sbjct: 320 NSILPNQSTFASGLNSCSALGTLDWGKEMHGVAVKLGLETDAFVGNSLVVMYSDSGNVND 379
Query: 310 EDDVFINIFE 319
VFI IF+
Sbjct: 380 AVSVFIKIFK 389
>At3g49900 putative protein
Length = 517
Score = 29.3 bits (64), Expect = 8.5
Identities = 13/33 (39%), Positives = 20/33 (60%)
Query: 62 SGSFSDIVVNAMGSTYHLHRLILSRSSYFRFEL 94
+ S D+ VN G+ +HLH+ LS+S Y + L
Sbjct: 63 NNSKPDVFVNVGGTRFHLHKDPLSKSGYLKRHL 95
>At3g22080 hypothetical protein
Length = 595
Score = 29.3 bits (64), Expect = 8.5
Identities = 18/66 (27%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Query: 253 DNNNRLVRDQSRDSFSSSSCSGLTSSEWGRYGTPLLSWGGHVGRRQVKAHPRGNYRDEDD 312
D N +R+ S+S + + G+Y + L + GG+ R + +P+GN +DE
Sbjct: 7 DANPSTLREHPPSSYSIKF-ENIAELDDGKYESSLFAAGGYNWRLVI--YPKGNAKDEGS 63
Query: 313 VFINIF 318
FI+++
Sbjct: 64 GFISMY 69
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.134 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,314,739
Number of Sequences: 26719
Number of extensions: 860573
Number of successful extensions: 3222
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 3202
Number of HSP's gapped (non-prelim): 28
length of query: 750
length of database: 11,318,596
effective HSP length: 107
effective length of query: 643
effective length of database: 8,459,663
effective search space: 5439563309
effective search space used: 5439563309
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 64 (29.3 bits)
Medicago: description of AC147012.12