BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147007.11 - phase: 0
(145 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03905 unknown protein 160 2e-40
At1g10500 unknown protein 54 4e-08
At2g36260 hypothetical protein 52 1e-07
At2g16710 putative HesB-like protein 48 2e-06
At2g36500 unknown protein (At2g36500) 31 0.20
At1g05200 putative glutamate receptor protein / ligand-gated ion... 30 0.58
At2g11890 pseudogene 29 1.00
At5g58370 contains similarity to GTP-binding protein CGPA 28 1.3
At4g18965 unknown protein 28 1.7
At4g08330 unknown protein 28 1.7
At1g70640 unknown protein 28 1.7
At5g20665 unknown protein 28 2.2
At4g09050 unknown protein 28 2.2
At3g24630 hypothetical protein 28 2.2
At1g55915 hypothetical protein 28 2.2
At4g30790 putative protein 27 2.9
At3g14950 hypothetical protein 27 2.9
At2g29630 putative thiamin biosynthesis protein 27 2.9
At2g24120 chloroplast single subunit DNA-dependent RNA polymerase 27 2.9
At1g21530 amp-binding protein, putative 27 2.9
>At5g03905 unknown protein
Length = 158
Score = 160 bits (406), Expect = 2e-40
Identities = 78/141 (55%), Positives = 103/141 (72%), Gaps = 2/141 (1%)
Query: 1 MQTSLFRRLAPLLAARFQRNQNIISSFSSSSSSSVLHHATSSPSSPDVEPVHITENCVRK 60
M SL +R+AP LA R + N +++ S+S+ ++S P S + VH+++NC+R+
Sbjct: 1 MSRSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRR 60
Query: 61 MKELDANESSSGGKMLRLSVETGGCSGFQYAFNLDDRFNSDDRVFEKEGIKLVVDNISYD 120
MKEL ++E KMLRL VETGGCSGFQY F LD+R N DDRVFEK G+KLVVDN+SYD
Sbjct: 61 MKELQSSEPEK--KMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYD 118
Query: 121 FVKGATVDYVEELIRSAFIVS 141
VKGAT+DY EELIR+AF+V+
Sbjct: 119 LVKGATIDYEEELIRAAFVVA 139
>At1g10500 unknown protein
Length = 180
Score = 53.5 bits (127), Expect = 4e-08
Identities = 32/109 (29%), Positives = 56/109 (51%), Gaps = 8/109 (7%)
Query: 39 ATSSPSSPDVE----PVHITENCVRKMKELDANESSSGGKMLRLSVETGGCSGFQYAFNL 94
+ S P++P +E + ++EN ++ + ++ + LR+ V+ GGCSG Y +
Sbjct: 56 SASVPAAPAMEGLKPAISLSENALKHLSKMRSERGED--LCLRIGVKQGGCSGMSYTMDF 113
Query: 95 DDRFNS--DDRVFEKEGIKLVVDNISYDFVKGATVDYVEELIRSAFIVS 141
++R N+ DD E +G +V D S F+ G +DY + LI F S
Sbjct: 114 ENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALIGGGFSFS 162
>At2g36260 hypothetical protein
Length = 109
Score = 51.6 bits (122), Expect = 1e-07
Identities = 24/64 (37%), Positives = 38/64 (58%)
Query: 76 LRLSVETGGCSGFQYAFNLDDRFNSDDRVFEKEGIKLVVDNISYDFVKGATVDYVEELIR 135
LRL+VE GC+G Y N D V E++G+K++VD + V G +D+V++ +R
Sbjct: 28 LRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVDPKAVMHVIGTEMDFVDDKLR 87
Query: 136 SAFI 139
S F+
Sbjct: 88 SEFV 91
>At2g16710 putative HesB-like protein
Length = 137
Score = 48.1 bits (113), Expect = 2e-06
Identities = 21/64 (32%), Positives = 38/64 (58%)
Query: 76 LRLSVETGGCSGFQYAFNLDDRFNSDDRVFEKEGIKLVVDNISYDFVKGATVDYVEELIR 135
LRL V+ GC+G Y N D D + E++G++++V+ + V G +D+V++ +R
Sbjct: 45 LRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104
Query: 136 SAFI 139
S F+
Sbjct: 105 SEFV 108
>At2g36500 unknown protein (At2g36500)
Length = 536
Score = 31.2 bits (69), Expect = 0.20
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Query: 23 IISSFSSSSSSSVLHHATSSPSSPDVEPVHITENCVRKM---KELDANESSS 71
++ S S S S ++ TS P SP +PV E V+K+ K L NE ++
Sbjct: 27 VLQSEESESGSGSINENTSKPDSPLAQPVSDGERTVKKLRLSKALTINEGTT 78
>At1g05200 putative glutamate receptor protein / ligand-gated ion
channel protein (AtGLR3.4)
Length = 959
Score = 29.6 bits (65), Expect = 0.58
Identities = 21/70 (30%), Positives = 34/70 (48%), Gaps = 9/70 (12%)
Query: 28 SSSSSSSVLHHATSSPSSPDVEPVHITENCVRK---------MKELDANESSSGGKMLRL 78
SSSSSSS L PSS +V + ++ + + M +++A++S G L +
Sbjct: 43 SSSSSSSSLRPLRQRPSSVNVGALFTYDSFIGRAAKPAVKAAMDDVNADQSVLKGIKLNI 102
Query: 79 SVETGGCSGF 88
+ CSGF
Sbjct: 103 IFQDSNCSGF 112
>At2g11890 pseudogene
Length = 210
Score = 28.9 bits (63), Expect = 1.00
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Query: 53 ITENCVRKMKELDANESSSGGKMLRLSVETGGCSGFQYAFNLDDRFNSDDRVFEKEGIKL 112
I + CV E A S G ++L+ E G + F F F + V+E G+KL
Sbjct: 99 IGKECV----ESPAKLSDIGSRVLKRVKEEYGFNDF-LGFVCLGGFENVRNVYEWRGVKL 153
Query: 113 VVDNISYDF 121
VD YDF
Sbjct: 154 EVDETKYDF 162
>At5g58370 contains similarity to GTP-binding protein CGPA
Length = 465
Score = 28.5 bits (62), Expect = 1.3
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 5/50 (10%)
Query: 18 QRNQNIISSFSSSSSSSVLHHATSSPSSPDVEPVHITENCVRKMKELDAN 67
+RNQ SSF +++ ++ T S S DV T RK ELD+N
Sbjct: 79 KRNQGSTSSFKTANDAATSVFKTKSRSGVDV-----TRKSYRKRDELDSN 123
>At4g18965 unknown protein
Length = 361
Score = 28.1 bits (61), Expect = 1.7
Identities = 21/84 (25%), Positives = 39/84 (46%), Gaps = 9/84 (10%)
Query: 36 LHHATSSPSSPDVEPVHITENCVRKMKELDANESSSGGKMLRLSVETGGCSGFQYAFN-- 93
++++T S SPD + + R ++L ++ K + + GCS + A N
Sbjct: 176 VYYSTGSQYSPDA---YANDLINRYTEQLRIMYNNGARKFALVGIGAIGCSPNELAQNSR 232
Query: 94 ----LDDRFNSDDRVFEKEGIKLV 113
D+R NS +R+F + + LV
Sbjct: 233 DGVTCDERINSANRIFNSKLVSLV 256
>At4g08330 unknown protein
Length = 164
Score = 28.1 bits (61), Expect = 1.7
Identities = 29/88 (32%), Positives = 38/88 (42%), Gaps = 15/88 (17%)
Query: 16 RFQRNQNIISSFSSSSSSSVLHHATSSPSSPDVEPVHITENCVRKMKELDANESSSGGKM 75
RF NQ SFSSSSSSS + H S S E + N + + S GK
Sbjct: 13 RFGGNQFYSPSFSSSSSSSSMRHVNYSCGSCGYELNLSSTN------RITSTIGSKYGKS 66
Query: 76 LRLSVETGGCSGFQYAFNLDD-RFNSDD 102
++ SG FN+D+ RF+ D
Sbjct: 67 MK--------SGIISFFNIDEGRFSQVD 86
>At1g70640 unknown protein
Length = 174
Score = 28.1 bits (61), Expect = 1.7
Identities = 16/42 (38%), Positives = 23/42 (54%)
Query: 9 LAPLLAARFQRNQNIISSFSSSSSSSVLHHATSSPSSPDVEP 50
L+PL + R N + S +SSSSS + SPS+P+ P
Sbjct: 105 LSPLKSTRTTANSSPPPSTTSSSSSKSRSRSPPSPSTPETCP 146
>At5g20665 unknown protein
Length = 301
Score = 27.7 bits (60), Expect = 2.2
Identities = 12/49 (24%), Positives = 31/49 (62%)
Query: 67 NESSSGGKMLRLSVETGGCSGFQYAFNLDDRFNSDDRVFEKEGIKLVVD 115
++ GG+++ +S++TGG S + ++D+ + +V E+E +L+++
Sbjct: 160 DKEREGGRVIAVSMDTGGSSRWTLRIDMDEIEDFTMQVGEEEEEELMIE 208
>At4g09050 unknown protein
Length = 304
Score = 27.7 bits (60), Expect = 2.2
Identities = 12/37 (32%), Positives = 25/37 (67%)
Query: 19 RNQNIISSFSSSSSSSVLHHATSSPSSPDVEPVHITE 55
+++N+ S FS+++ VL ++SS S+P+V I++
Sbjct: 57 KSRNLTSYFSTTTQEPVLESSSSSSSAPEVVEEEISK 93
>At3g24630 hypothetical protein
Length = 724
Score = 27.7 bits (60), Expect = 2.2
Identities = 15/47 (31%), Positives = 26/47 (54%)
Query: 2 QTSLFRRLAPLLAARFQRNQNIISSFSSSSSSSVLHHATSSPSSPDV 48
QT+ ++ + F + +SS +SSS SS++ +T S +S DV
Sbjct: 198 QTTTIETKKRVVRSDFASSSGAVSSSTSSSQSSMVSGSTKSSASSDV 244
>At1g55915 hypothetical protein
Length = 424
Score = 27.7 bits (60), Expect = 2.2
Identities = 17/68 (25%), Positives = 27/68 (39%)
Query: 3 TSLFRRLAPLLAARFQRNQNIISSFSSSSSSSVLHHATSSPSSPDVEPVHITENCVRKMK 62
+S+ L+P+ AA + ++ S S+ + S EPV I E C
Sbjct: 232 SSIMSDLSPIQAAAMAAERRLLDDIWCGSQSTDALEDEENDSDTYKEPVSIRETCTSGSD 291
Query: 63 ELDANESS 70
+D E S
Sbjct: 292 VIDLTEES 299
>At4g30790 putative protein
Length = 1148
Score = 27.3 bits (59), Expect = 2.9
Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 1/65 (1%)
Query: 20 NQNIISSFSSSSSSSVL-HHATSSPSSPDVEPVHITENCVRKMKELDANESSSGGKMLRL 78
N+N + + SS S ++ H S P+ + VHI V+ +L A+ S GK L
Sbjct: 1036 NRNCPNYYLSSESEALFTDHLPSRPTYIVGQIVHIERQIVKLPSQLSASASPEAGKTHHL 1095
Query: 79 SVETG 83
+ G
Sbjct: 1096 CSDQG 1100
>At3g14950 hypothetical protein
Length = 721
Score = 27.3 bits (59), Expect = 2.9
Identities = 18/31 (58%), Positives = 20/31 (64%), Gaps = 4/31 (12%)
Query: 25 SSFSSSSSSSVLHHATSSP---SSPD-VEPV 51
SSFSSSSS SV HA +P PD V+PV
Sbjct: 58 SSFSSSSSGSVTGHAGHTPPVTRKPDSVQPV 88
>At2g29630 putative thiamin biosynthesis protein
Length = 644
Score = 27.3 bits (59), Expect = 2.9
Identities = 18/55 (32%), Positives = 25/55 (44%), Gaps = 1/55 (1%)
Query: 40 TSSPSSPDVEPVHITENCVRK-MKELDANESSSGGKMLRLSVETGGCSGFQYAFN 93
T PSSPD +P+ E C K KE G +L++ SG + AF+
Sbjct: 74 TIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRVHLSGGEPAFD 128
>At2g24120 chloroplast single subunit DNA-dependent RNA polymerase
Length = 993
Score = 27.3 bits (59), Expect = 2.9
Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 10/98 (10%)
Query: 3 TSLFRRLAPLLAARFQRNQNIISSFSSSSSSSVLHHATSSPSSPDVEPVHITENCVRKMK 62
++LFRR F+R+ IS+ SSSSSSS + S P VH N + +
Sbjct: 35 SALFRRKT---LPFFERHSLPISASSSSSSSS--STSLSVHEKPISNSVHFHGNLIESFE 89
Query: 63 ELDAN-----ESSSGGKMLRLSVETGGCSGFQYAFNLD 95
D++ + +S + L VE G SG + F D
Sbjct: 90 NQDSSYAGTIKGASLIEELENPVERNGLSGRRRLFMQD 127
>At1g21530 amp-binding protein, putative
Length = 776
Score = 27.3 bits (59), Expect = 2.9
Identities = 12/35 (34%), Positives = 20/35 (56%)
Query: 62 KELDANESSSGGKMLRLSVETGGCSGFQYAFNLDD 96
K L+A E + G ++L + E+GG Q N+D+
Sbjct: 671 KVLEATEGTGGEEVLEAASESGGLEAAQKRVNIDE 705
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.131 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,171,349
Number of Sequences: 26719
Number of extensions: 136827
Number of successful extensions: 736
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 709
Number of HSP's gapped (non-prelim): 42
length of query: 145
length of database: 11,318,596
effective HSP length: 90
effective length of query: 55
effective length of database: 8,913,886
effective search space: 490263730
effective search space used: 490263730
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC147007.11