BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146856.2 + phase: 0
(88 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14640 putative retroelement pol polyprotein 44 1e-05
At2g13370 pseudogene 31 0.094
At1g21530 amp-binding protein, putative 30 0.27
At2g10780 pseudogene 29 0.36
At3g11970 hypothetical protein 29 0.47
At2g17480 membrane protein Mlo8 29 0.47
At1g36590 hypothetical protein 29 0.47
At4g31900 putative protein 28 0.80
At1g50020 tubulin alpha-6 chain like protein 28 0.80
At5g44800 helicase-like protein 28 1.0
At4g19020 unknown protein 27 1.8
At4g04885 unknown protein 27 2.3
At1g58350 putative protein 27 2.3
At5g35070 putative protein 26 3.0
At5g08010 putative protein 26 3.0
At1g35370 hypothetical protein 26 4.0
At5g55660 putative protein 25 5.2
At3g55590 mannose-1-phosphate guanylyltransferase-like protein 25 5.2
At2g33800 30S ribosomal protein S5 25 5.2
At1g80740 chromomethylase (CMT1) 25 5.2
>At2g14640 putative retroelement pol polyprotein
Length = 945
Score = 44.3 bits (103), Expect = 1e-05
Identities = 24/56 (42%), Positives = 33/56 (58%), Gaps = 2/56 (3%)
Query: 4 QPLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWPSPI--LEDKV 57
QP VL R + +LVQW+GL E+ATWE+ D++ S+P + LEDKV
Sbjct: 888 QPTAVLEVRWRSQDKKRVADLLVQWEGLHIEDATWEEYDQLAASFPEFVLNLEDKV 943
>At2g13370 pseudogene
Length = 1738
Score = 31.2 bits (69), Expect = 0.094
Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 15/60 (25%)
Query: 23 QMLVQWKGLSPEEATWEDE----------DEMRTSWPSPILEDKVVE-----GDGNDTYS 67
+ LV+W+GLS EATWE + DE + S ++ K+VE G G +++S
Sbjct: 554 EYLVKWQGLSYAEATWEKDVDIAFAQVAIDEYKAREVSIAVQGKMVEQQRTKGKGENSFS 613
>At1g21530 amp-binding protein, putative
Length = 776
Score = 29.6 bits (65), Expect = 0.27
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 3/35 (8%)
Query: 5 PLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWE 39
P KVL TR + +G+ + LVQW+GL E +WE
Sbjct: 619 PEKVLDTRY--SAEGV-LEALVQWQGLPTHETSWE 650
>At2g10780 pseudogene
Length = 1611
Score = 29.3 bits (64), Expect = 0.36
Identities = 21/82 (25%), Positives = 35/82 (42%), Gaps = 8/82 (9%)
Query: 5 PLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWPSPILEDKVVEGDGND 64
P++++ + T + + V W EE TWE E++M+ ++P E G D
Sbjct: 1535 PVRIMDRMTKGTRGKARDLLKVLWNCRGREEYTWETENKMKANFPEWFKE------MGKD 1588
Query: 65 TYSLDSKHATEPGIRNEQLNTK 86
DS+ T P E N +
Sbjct: 1589 QLDADSR--TNPIQGGETCNAR 1608
>At3g11970 hypothetical protein
Length = 1499
Score = 28.9 bits (63), Expect = 0.47
Identities = 14/45 (31%), Positives = 25/45 (55%)
Query: 5 PLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWP 49
P KV+ + + T++LV+W EEATWE +++ ++P
Sbjct: 1451 PEKVVERKMVNRQGKAVTKVLVKWSNEPLEEATWEFLFDLQKTFP 1495
>At2g17480 membrane protein Mlo8
Length = 593
Score = 28.9 bits (63), Expect = 0.47
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 1/69 (1%)
Query: 8 VLGTRSLRTPQGIKTQM-LVQWKGLSPEEATWEDEDEMRTSWPSPILEDKVVEGDGNDTY 66
V T S+R+ Q KT ++++GL PE + + ++E T SP + VV+ + N T
Sbjct: 504 VRSTSSVRSLQRYKTTPHSMRYEGLDPETSDLDTDNEALTPPKSPPSFELVVKVEPNKTN 563
Query: 67 SLDSKHATE 75
+ ++ TE
Sbjct: 564 TGETSRDTE 572
>At1g36590 hypothetical protein
Length = 1499
Score = 28.9 bits (63), Expect = 0.47
Identities = 14/45 (31%), Positives = 25/45 (55%)
Query: 5 PLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWP 49
P KV+ + + T++LV+W EEATWE +++ ++P
Sbjct: 1451 PEKVVERKMVNRQGKAVTKVLVKWSNEPLEEATWEFLFDLQKTFP 1495
>At4g31900 putative protein
Length = 1067
Score = 28.1 bits (61), Expect = 0.80
Identities = 12/28 (42%), Positives = 16/28 (56%)
Query: 23 QMLVQWKGLSPEEATWEDEDEMRTSWPS 50
Q LV+WKGLS +W E E ++ S
Sbjct: 78 QYLVKWKGLSYLHCSWVPEQEFEKAYKS 105
>At1g50020 tubulin alpha-6 chain like protein
Length = 209
Score = 28.1 bits (61), Expect = 0.80
Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 4/65 (6%)
Query: 27 QWKGLSPEEATWEDEDEMRTSWPSPILEDKVVEGD----GNDTYSLDSKHATEPGIRNEQ 82
Q KG P E+ + E + R P+ +L+D V+ D G +T + AT P
Sbjct: 58 QSKGEEPPESLFMKELKRRGMTPTSLLQDYEVDQDEIKTGKETGNSSKTTATTPAFDKSL 117
Query: 83 LNTKK 87
LN ++
Sbjct: 118 LNQRE 122
>At5g44800 helicase-like protein
Length = 2228
Score = 27.7 bits (60), Expect = 1.0
Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
Query: 4 QPLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWEDEDE 43
QP +++ +LR + + V+W GL+ +E TWE +E
Sbjct: 588 QPQRIV---ALRVSKEGNQEAYVKWTGLAYDECTWESLEE 624
>At4g19020 unknown protein
Length = 1171
Score = 26.9 bits (58), Expect = 1.8
Identities = 10/20 (50%), Positives = 13/20 (65%)
Query: 26 VQWKGLSPEEATWEDEDEMR 45
V WKG +E TWE +E+R
Sbjct: 811 VHWKGYRSDEDTWELAEELR 830
>At4g04885 unknown protein
Length = 808
Score = 26.6 bits (57), Expect = 2.3
Identities = 9/29 (31%), Positives = 16/29 (55%)
Query: 12 RSLRTPQGIKTQMLVQWKGLSPEEATWED 40
+ +R G+ ++M+ W+ EE WED
Sbjct: 331 KPIRDMNGMHSKMVTPWQNTEEEEFDWED 359
>At1g58350 putative protein
Length = 794
Score = 26.6 bits (57), Expect = 2.3
Identities = 12/45 (26%), Positives = 22/45 (48%), Gaps = 6/45 (13%)
Query: 38 WEDEDEMRTSWPSPILEDKVVEGDGNDTYSL------DSKHATEP 76
WED D + PS +++ + + ND+Y + D+ T+P
Sbjct: 68 WEDGDNVTRGIPSRVVQYEAPDSGANDSYGVWKIVDKDNSFLTQP 112
>At5g35070 putative protein
Length = 356
Score = 26.2 bits (56), Expect = 3.0
Identities = 17/58 (29%), Positives = 24/58 (41%), Gaps = 3/58 (5%)
Query: 13 SLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWPSPILEDKVVEGDGNDTYSLDS 70
++R +V W G E ATW T+W L +++ EGDG L S
Sbjct: 138 TMRESSASMVDWIVSWWGTGDELATWRRACCNSTNWR---LGNELDEGDGGYRVGLRS 192
>At5g08010 putative protein
Length = 566
Score = 26.2 bits (56), Expect = 3.0
Identities = 17/42 (40%), Positives = 21/42 (49%), Gaps = 2/42 (4%)
Query: 13 SLRTPQGIKTQMLVQWKGLSPEEATWEDEDEMRTSWPSPILE 54
S RT + + ML GLS EE E+EDEM I+E
Sbjct: 501 SSRTSEDVNLSMLQDLLGLSDEEE--EEEDEMEKHLIKQIVE 540
>At1g35370 hypothetical protein
Length = 1447
Score = 25.8 bits (55), Expect = 4.0
Identities = 12/35 (34%), Positives = 19/35 (54%)
Query: 5 PLKVLGTRSLRTPQGIKTQMLVQWKGLSPEEATWE 39
P +L + ++ T +LV+W G EEATW+
Sbjct: 1399 PEYILERKLVKRQGRAATMVLVKWIGEPVEEATWK 1433
>At5g55660 putative protein
Length = 759
Score = 25.4 bits (54), Expect = 5.2
Identities = 18/60 (30%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Query: 29 KGLSPEEATWEDEDEMRTSWPSPILEDKVVEGDGNDTYSLDSKHATEPGIRNEQLNTKKE 88
KG +EA ED+ E T P +EDK E ++ + + E E+ N KE
Sbjct: 211 KGEEVKEANKEDDVEADTKVAEPEVEDKKTESK-DENEDKEEEKEDEKEDEKEESNDDKE 269
>At3g55590 mannose-1-phosphate guanylyltransferase-like protein
Length = 364
Score = 25.4 bits (54), Expect = 5.2
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 7/55 (12%)
Query: 23 QMLVQWKGLSPEEATW-------EDEDEMRTSWPSPILEDKVVEGDGNDTYSLDS 70
Q+LV K + EAT ++ + + T+ P + DK+V+G G + L+S
Sbjct: 59 QLLVMSKFSNDVEATLGIKITCSQETEPLGTAGPLALARDKLVDGSGQPFFVLNS 113
>At2g33800 30S ribosomal protein S5
Length = 303
Score = 25.4 bits (54), Expect = 5.2
Identities = 16/48 (33%), Positives = 24/48 (49%), Gaps = 5/48 (10%)
Query: 34 EEATWEDEDEMRTSWPSPILEDKVVEGD---GNDTYSLDSKHATEPGI 78
+E + E E+E+ T++ L G+ G D Y +DSKH GI
Sbjct: 89 DEGSDETEEEIATAFEE--LYGPAYSGESMLGKDIYVMDSKHKKSSGI 134
>At1g80740 chromomethylase (CMT1)
Length = 791
Score = 25.4 bits (54), Expect = 5.2
Identities = 13/26 (50%), Positives = 17/26 (65%), Gaps = 3/26 (11%)
Query: 17 PQGI--KT-QMLVQWKGLSPEEATWE 39
PQG KT Q++V+WKG + TWE
Sbjct: 352 PQGTGEKTLQLMVRWKGYNSSYDTWE 377
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.307 0.128 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,238,704
Number of Sequences: 26719
Number of extensions: 92425
Number of successful extensions: 180
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 161
Number of HSP's gapped (non-prelim): 27
length of query: 88
length of database: 11,318,596
effective HSP length: 64
effective length of query: 24
effective length of database: 9,608,580
effective search space: 230605920
effective search space used: 230605920
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146856.2