BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146794.13 + phase: 0 /pseudo
(818 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g04670 putative retroelement pol polyprotein 102 1e-21
At4g03650 putative reverse transcriptase 101 2e-21
At2g14650 putative retroelement pol polyprotein 99 1e-20
At2g10780 pseudogene 99 1e-20
At4g10580 putative reverse-transcriptase -like protein 97 5e-20
At2g07660 putative retroelement pol polyprotein 95 2e-19
At3g11970 hypothetical protein 84 4e-16
At1g36590 hypothetical protein 84 4e-16
At2g05610 putative retroelement pol polyprotein 81 3e-15
At4g03840 putative transposon protein 79 1e-14
At2g06470 putative retroelement pol polyprotein 79 1e-14
At2g06890 putative retroelement integrase 78 2e-14
At4g08100 putative polyprotein 77 3e-14
At1g35370 hypothetical protein 65 2e-10
At4g04230 putative transposon protein 58 2e-08
At2g15410 putative retroelement pol polyprotein 55 2e-07
At4g03800 55 2e-07
At2g14400 putative retroelement pol polyprotein 53 6e-07
At2g14380 putative retroelement pol polyprotein 51 2e-06
At1g20390 hypothetical protein 51 3e-06
>At2g04670 putative retroelement pol polyprotein
Length = 1411
Score = 102 bits (253), Expect = 1e-21
Identities = 44/84 (52%), Positives = 63/84 (74%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F +LD FV++FIDDIL+YS+S EEH HL+ +++ L+E+K++AKLSKC F
Sbjct: 649 AFMRLMNSVFQEFLDEFVIIFIDDILVYSKSPEEHEVHLRRVMEKLREQKLFAKLSKCSF 708
Query: 78 WLSEVSFLGHIISDSGIVVTHQKL 101
W E+ FLGHI+S G+ V +K+
Sbjct: 709 WQREIGFLGHIVSAEGVSVDPEKI 732
>At4g03650 putative reverse transcriptase
Length = 839
Score = 101 bits (251), Expect = 2e-21
Identities = 44/84 (52%), Positives = 63/84 (74%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F +LD FV++FIDDIL+YS+S EEH HL+ +++ L+E+K++AKLSKC F
Sbjct: 548 AFMRLMNSVFQEFLDEFVIIFIDDILVYSKSPEEHEVHLRRVMEKLREQKLFAKLSKCSF 607
Query: 78 WLSEVSFLGHIISDSGIVVTHQKL 101
W E+ FLGHI+S G+ V +K+
Sbjct: 608 WQREMGFLGHIVSAEGVSVDPEKI 631
>At2g14650 putative retroelement pol polyprotein
Length = 1328
Score = 99.0 bits (245), Expect = 1e-20
Identities = 44/84 (52%), Positives = 62/84 (73%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F LD FV++FIDDIL+YS+S EEH HL+ +++ L+E+K++AKLSKC F
Sbjct: 598 AFMRLMNSVFQEVLDEFVIIFIDDILVYSKSLEEHEVHLRRVMEKLREQKLFAKLSKCSF 657
Query: 78 WLSEVSFLGHIISDSGIVVTHQKL 101
W E+ FLGHI+S G+ V +K+
Sbjct: 658 WQREMGFLGHIVSAEGVSVDPEKI 681
>At2g10780 pseudogene
Length = 1611
Score = 99.0 bits (245), Expect = 1e-20
Identities = 44/84 (52%), Positives = 62/84 (73%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F +LD FV++FI+DIL+YS+S E H EHL+ +L+ L+E +++AKLSKC F
Sbjct: 791 AFMKMMNGVFRDFLDEFVIIFINDILVYSKSWEAHQEHLRAVLERLREHELFAKLSKCSF 850
Query: 78 WLSEVSFLGHIISDSGIVVTHQKL 101
W V FLGH+ISD G+ V +K+
Sbjct: 851 WQRSVGFLGHVISDQGVSVDPEKI 874
>At4g10580 putative reverse-transcriptase -like protein
Length = 1240
Score = 96.7 bits (239), Expect = 5e-20
Identities = 42/83 (50%), Positives = 61/83 (72%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
F MN +F +LD FV++FIDDIL+YS+S EE HL+ +++ L+E+K++AKLSKC FW
Sbjct: 624 FMRLMNSVFQEFLDEFVIIFIDDILVYSKSPEEQEVHLRRVMEKLREQKLFAKLSKCSFW 683
Query: 79 LSEVSFLGHIISDSGIVVTHQKL 101
E+ FLGHI+S G+ V +K+
Sbjct: 684 QREMGFLGHIVSAEGVSVDPEKI 706
>At2g07660 putative retroelement pol polyprotein
Length = 949
Score = 94.7 bits (234), Expect = 2e-19
Identities = 43/84 (51%), Positives = 61/84 (72%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F +LD FV++FIDDIL++S+S E H EHL+ +L+ L+E +++AKLSK F
Sbjct: 286 AFMKMMNGVFRDFLDEFVIIFIDDILVHSKSWEAHQEHLRAVLERLREHELFAKLSKFSF 345
Query: 78 WLSEVSFLGHIISDSGIVVTHQKL 101
W V FLGH+ISD G+ V +K+
Sbjct: 346 WQRSVGFLGHVISDQGVSVDPEKI 369
>At3g11970 hypothetical protein
Length = 1499
Score = 83.6 bits (205), Expect = 4e-16
Identities = 41/83 (49%), Positives = 56/83 (67%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
F+ MN IF +L FV+VF DDIL+YS S EEH +HLK + +V++ K++AKLSKC F
Sbjct: 734 FQGLMNFIFKPFLRKFVLVFFDDILVYSSSLEEHRQHLKQVFEVMRANKLFAKLSKCAFA 793
Query: 79 LSEVSFLGHIISDSGIVVTHQKL 101
+ +V +LGH IS GI K+
Sbjct: 794 VPKVEYLGHFISAQGIETDPAKI 816
>At1g36590 hypothetical protein
Length = 1499
Score = 83.6 bits (205), Expect = 4e-16
Identities = 41/83 (49%), Positives = 56/83 (67%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
F+ MN IF +L FV+VF DDIL+YS S EEH +HLK + +V++ K++AKLSKC F
Sbjct: 734 FQGLMNFIFKPFLRKFVLVFFDDILVYSSSLEEHRQHLKQVFEVMRANKLFAKLSKCAFA 793
Query: 79 LSEVSFLGHIISDSGIVVTHQKL 101
+ +V +LGH IS GI K+
Sbjct: 794 VPKVEYLGHFISAQGIETDPAKI 816
>At2g05610 putative retroelement pol polyprotein
Length = 780
Score = 80.9 bits (198), Expect = 3e-15
Identities = 38/84 (45%), Positives = 54/84 (64%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
+F+ MN F +L FV+VF DDILIYS S EEH +HL+ + +V++ ++AK+SKC F
Sbjct: 175 SFQSLMNSFFKPFLRKFVLVFFDDILIYSTSMEEHKKHLEAVFEVMRVHHLFAKMSKCAF 234
Query: 78 WLSEVSFLGHIISDSGIVVTHQKL 101
+ V +LGH IS GI K+
Sbjct: 235 AVPRVEYLGHFISGEGIATDPAKI 258
>At4g03840 putative transposon protein
Length = 973
Score = 78.6 bits (192), Expect = 1e-14
Identities = 34/61 (55%), Positives = 47/61 (76%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F +LD FV++FIDDIL+YS+S E H EHL+ +L+ L+E +++AKLSKC F
Sbjct: 315 AFMKMMNGVFRDFLDEFVIIFIDDILVYSKSWEAHQEHLRAVLEQLREHELFAKLSKCSF 374
Query: 78 W 78
W
Sbjct: 375 W 375
>At2g06470 putative retroelement pol polyprotein
Length = 899
Score = 78.6 bits (192), Expect = 1e-14
Identities = 38/70 (54%), Positives = 51/70 (72%), Gaps = 2/70 (2%)
Query: 18 AFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
AF MN +F +LD FV++FIDDIL+YS+S E H EHL+ +L+ L+E +++AKLSKC F
Sbjct: 315 AFMKMMNGVFRDFLDEFVIIFIDDILVYSKSWEAHQEHLRAVLERLREHELFAKLSKCSF 374
Query: 78 W--LSEVSFL 85
W E SFL
Sbjct: 375 WQRSCEKSFL 384
>At2g06890 putative retroelement integrase
Length = 1215
Score = 78.2 bits (191), Expect = 2e-14
Identities = 35/83 (42%), Positives = 53/83 (63%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
F MN + A++ FV+V+ DDIL+YS S EH EHL +L VL+++++YA L KC F
Sbjct: 552 FMRLMNHVLRAFIGIFVIVYFDDILVYSESLREHIEHLDSVLNVLRKEELYANLKKCTFC 611
Query: 79 LSEVSFLGHIISDSGIVVTHQKL 101
+ FLG ++S G+ V +K+
Sbjct: 612 TDNLVFLGFVVSADGVKVDEEKV 634
>At4g08100 putative polyprotein
Length = 1054
Score = 77.4 bits (189), Expect = 3e-14
Identities = 34/85 (40%), Positives = 55/85 (64%)
Query: 17 DAFRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCE 76
+ F MN + ++ FV+V+ DDIL+YS++ E H HLK++L +L+++K+YA L KC
Sbjct: 603 NTFMRLMNHVLRKHIGVFVIVYFDDILVYSKNLEYHVMHLKLVLDLLRKEKLYANLKKCT 662
Query: 77 FWLSEVSFLGHIISDSGIVVTHQKL 101
F + FLG ++S GI V +K+
Sbjct: 663 FCTDNLVFLGFVVSADGIKVDEEKV 687
>At1g35370 hypothetical protein
Length = 1447
Score = 64.7 bits (156), Expect = 2e-10
Identities = 34/72 (47%), Positives = 46/72 (63%), Gaps = 8/72 (11%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
F+ MN +F +L FV+VF DDILIYS S EEH EHL+++ +V++ K++AK SK
Sbjct: 713 FQSLMNSVFRDFLRKFVLVFFDDILIYSSSIEEHKEHLRLVFEVMRLHKLFAKGSK---- 768
Query: 79 LSEVSFLGHIIS 90
LGH IS
Sbjct: 769 ----EHLGHFIS 776
>At4g04230 putative transposon protein
Length = 315
Score = 58.2 bits (139), Expect = 2e-08
Identities = 24/59 (40%), Positives = 42/59 (70%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEF 77
F MN++ +++ FVVV+ DDILIY++S +H + L+++L+ L+++ +YA L KC F
Sbjct: 234 FMRLMNQVLRSFIGKFVVVYFDDILIYNKSYSDHIQPLELILKTLRKEGLYANLKKCTF 292
>At2g15410 putative retroelement pol polyprotein
Length = 1787
Score = 55.1 bits (131), Expect = 2e-07
Identities = 28/72 (38%), Positives = 42/72 (57%)
Query: 23 MNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFWLSEV 82
+N++F LD + V+IDD+L+ S EEH HL+ QVL M SKC F ++
Sbjct: 920 VNQMFTDQLDHSMEVYIDDMLVKSLRAEEHITHLRQCFQVLNRYNMKLNPSKCTFGVTSG 979
Query: 83 SFLGHIISDSGI 94
FLG++++ GI
Sbjct: 980 EFLGYLVTRRGI 991
>At4g03800
Length = 637
Score = 54.7 bits (130), Expect = 2e-07
Identities = 26/76 (34%), Positives = 46/76 (60%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
++ +NK+F+ +L + V IDD L+ S +E+H +HL ++L + +M L+KC F
Sbjct: 460 YQWLVNKMFNEHLRKTMEVSIDDTLVKSLKKEDHVKHLGECFEILNQYQMKLNLAKCTFG 519
Query: 79 LSEVSFLGHIISDSGI 94
+ FLG+I++ GI
Sbjct: 520 VPSGEFLGYIVTKRGI 535
>At2g14400 putative retroelement pol polyprotein
Length = 1466
Score = 53.1 bits (126), Expect = 6e-07
Identities = 25/72 (34%), Positives = 44/72 (60%)
Query: 23 MNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFWLSEV 82
+NK+F ++ + V+IDD+LI S +E+H +HL+ +L + +M +KC F +
Sbjct: 602 VNKMFSEHVGKTMEVYIDDMLIKSLKKEDHVKHLEECFAILNQYQMKLNPAKCTFGVPSG 661
Query: 83 SFLGHIISDSGI 94
FLG+I++ GI
Sbjct: 662 EFLGYIVTKRGI 673
>At2g14380 putative retroelement pol polyprotein
Length = 764
Score = 51.2 bits (121), Expect = 2e-06
Identities = 28/97 (28%), Positives = 53/97 (53%), Gaps = 3/97 (3%)
Query: 23 MNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFWLSEV 82
+N++F L + V+IDD+L+ S +H +HLK + L + M +KC F ++
Sbjct: 635 VNQMFAPQLGKTMEVYIDDMLVKSTRSADHIDHLKACFETLNKYNMKLNPAKCLFGVTSG 694
Query: 83 SFLGHIISDSGIVVTHQK---LMQ*HNGRLRSQLQRL 116
FLG+I++ GI ++ ++ + R + ++QRL
Sbjct: 695 EFLGYIVTKRGIEANPKQIRAILDLQSPRNKKEVQRL 731
>At1g20390 hypothetical protein
Length = 1791
Score = 50.8 bits (120), Expect = 3e-06
Identities = 25/76 (32%), Positives = 42/76 (54%)
Query: 19 FRCYMNKIFHAYLDWFVVVFIDDILIYSRSEEEHAEHLKIMLQVLKEKKMYAKLSKCEFW 78
++ ++NK+ + V V+IDD+L+ S E+H EHL VL M +KC F
Sbjct: 911 YQRFVNKMLADQIGRTVEVYIDDMLVKSLKPEDHVEHLSKCFDVLNTYGMKLNPTKCTFG 970
Query: 79 LSEVSFLGHIISDSGI 94
++ FLG++++ GI
Sbjct: 971 VTSGEFLGYVVTKRGI 986
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.372 0.167 0.652
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,058,623
Number of Sequences: 26719
Number of extensions: 480117
Number of successful extensions: 2226
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2182
Number of HSP's gapped (non-prelim): 44
length of query: 818
length of database: 11,318,596
effective HSP length: 108
effective length of query: 710
effective length of database: 8,432,944
effective search space: 5987390240
effective search space used: 5987390240
T: 11
A: 40
X1: 14 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.9 bits)
S2: 64 (29.3 bits)
Medicago: description of AC146794.13